Status
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Gene information
General information
TC011206 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 2281364..2305660
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011206
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04986

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    Pupal morphology
    wings size shortened - Number of animals showing the phenotype: 7

    oblique dorsal thoracic muscles fragmented - Number of animals showing the phenotype: 7

    Comment: the outer one of the two oblique muscles is fragented, the median one looks normal
    Adult morphology
    elytra not closed - Number of animals showing the phenotype: 2


    elytra surface irregular - Number of animals showing the phenotype: 2


    elytra size shortened - Number of animals showing the phenotype: 3


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011206 RA
      MSSSDPPTD QDDASRINQ EASSSTNNE TSTPDNKED 
      EMKRAAARK LIERYFYQL TDGCGNPAC KNKYCASSG 
      QLKPLTPDE AAAQAIQLF SQEAILCER HPNKIQRTN 
      NKMDNDHKM LSSDSDESI NRKTGLTPN NEILSGNES 
      YIKLNTTNN STSYLTEQM LYDIIDECE STECYLSLI 
      RKLGEVFSS IENLSRSFQ QSDKSSPSE ANLDETGLK 
      KEDVRSLEG EKDKDEDSS AETPDGGVL PVDLPSVRR 
      AYSALFKLK TSIFEHSLV NALVTLAGN LQMELPTRK 
      ERGNQDDVV NVLLIVFEI PALGSGDFL ETALPAICR 
      ASEWLPVEV QAKLARIWS GTGRSSLRN ILENLQQLV 
      TLRVIVTQF HRDFYVQDE NVITSATKL MKILFYANI 
      LAGVLESPD LRNEDLSMS MDDSYVAIK LNKTQPPVD 
      PLAAELGVH VLDCRKPYL PFSEFYNEP LSDGVEMDR 
      DFANYKSEL GKFSFMNYP FILTPATKM MGLYFDNRI 
      RMYSERRIS ILQSVTGQP SNPYLRLKV RRDHIIDDA 
      LVELEMISM ENPNDLKKQ LVVEFEGEQ GIDEGGVSK 
      EFFQLVIEE IFNPDYAMF TSQSETGTV WFNPTSFES 
      DAQFTLIGI VLGLAIYNN VILAVNFPM VLYRKLLGK 
      RGSFEDLQD WNLTLYNSL KQLLEYNEP DVEEVFMQT 
      FRISYQDVF GSIINYDLK DRGDEINVT QENKYEFVD 
      LYADFLLNK SVEKQFRAF YKGFQMVVD ESPLELLFR 
      PEEIEILIC GSKNFDFDE LESSTEYDG GYTSESQII 
      KDFWSIVHA LSLEDKRKL LQFTTGSDR VPIGGLSRL 
      KLVIARNGP DSDRLPTAH TCFNVLLLP EYSSKEKLK 
      DRLIKAISY SKGFGML
    CDS sequence
    • >TC011206 RA
      atgagctct tcagaccca ccaactgac caagacgac 
      gcttcaagg ataaaccaa gaggcttcg tctagcacc 
      aacaacgaa acctctacc cctgacaat aaggaggac 
      gagatgaaa cgtgctgct gcccgcaag ctgattgaa 
      cgatatttc taccaattg accgacggt tgtgggaac 
      cccgcctgc aagaacaag tactgcgcc tccagcgga 
      cagctcaag ccactcacg ccagacgag gcggctgcg 
      caagccatt cagctcttc tctcaagaa gcgattttg 
      tgcgagaga caccctaac aaaatacag aggactaac 
      aataaaatg gataatgac cacaaaatg ctttcgtcg 
      gattccgac gagtcaatc aatcgcaaa acgggcctc 
      acaccaaat aacgagatt ttatctggc aatgaaagc 
      tatatcaag ctcaacact accaacaac tcgactagt 
      tatttgacg gaacaaatg ctttacgac ataatcgac 
      gagtgcgaa tcgaccgaa tgctacttg agtctaatc 
      cgaaaactg ggcgaagtc ttcagcagc atagaaaac 
      ctgtctcgg agcttccaa cagagcgac aagagcagc 
      cccagtgag gcaaacctg gacgaaacg ggcctcaaa 
      aaggaggac gtgcgctcg ctcgagggc gagaaagac 
      aaggacgaa gactcctcg gcggagacc cccgatggg 
      ggcgtcctc cccgtcgac ctcccctcg gtccgacgc 
      gcttactcg gccctcttc aagctgaaa acgtcgatt 
      ttcgagcac tctctggtc aacgcgttg gttactttg 
      gccggcaac ctccagatg gagctgccg acgcggaaa 
      gagcgcggc aaccaggac gatgtggtc aatgttttg 
      ttgattgtt tttgaaatt cccgcgctc ggttcgggg 
      gatttcctc gaaacggcg ttgccggcg atctgtcgg 
      gcttccgaa tggctgccg gtggaagtc caagcgaag 
      ttggcgcgg atttggtcg gggacggga cgctcgagt 
      cttaggaac attttggag aatttgcaa cagttggtg 
      acactgagg gtgatcgtg acgcagttt catagggat 
      ttttatgtt caggatgag aatgttatc acgtcggct 
      acgaaatta atgaagatt cttttttat gcgaatatt 
      ttggcgggg gttttggaa tcgcctgat ttgcggaat 
      gaagatttg tcaatgtcc atggatgat tcgtatgtt 
      gctatcaaa ttgaataaa acgcaacca ccggttgat 
      cctttggct gccgagttg ggagtgcac gttttggac 
      tgtcgcaaa ccttattta ccattttct gaattctat 
      aatgagcct ttgagtgat ggtgtcgag atggatcga 
      gactttgca aattataag agcgaactt ggaaagttt 
      agttttatg aattatccc tttattttg acgccggcg 
      accaaaatg atgggttta tatttcgat aaccggatt 
      cggatgtat tcagaacga cggattagt attttacag 
      tcggttaca ggccaacct tccaaccca tatttgagg 
      ctgaaggtt aggagggat cacataatt gacgatgcc 
      ttggttgag ctggagatg atatctatg gagaacccc 
      aacgatttg aagaagcag ttggtggtg gagttcgaa 
      ggcgagcaa ggaattgac gaaggcggc gtgtcaaag 
      gaattcttt cagttagtg atcgaagaa attttcaat 
      cccgactat gcgatgttt acgagtcag agtgagaca 
      ggcacagtt tggttcaat cccactagt ttcgaaagt 
      gacgctcaa ttcacatta attggaata gtgttagga 
      cttgccatt tataataat gttatcttg gccgtgaat 
      ttccctatg gtactttac aggaaactg ttaggtaaa 
      aggggctca tttgaagac ttacaagac tggaatctt 
      actttatat aatagttta aaacaatta ctggaatat 
      aacgagcct gacgttgag gaagttttt atgcaaacg 
      ttccgaatt agctaccaa gatgtgttc ggttctatt 
      ataaactac gacttaaaa gatcgcggc gacgaaatt 
      aacgtaaca caagaaaat aaatatgaa tttgtagat 
      ttgtacgcc gatttctta ctaaataaa tcagtagag 
      aaacagttt cgtgccttt tacaaaggg ttccaaatg 
      gtcgtagac gagtcaccg ctggaatta ttgttccgt 
      cccgaagaa attgaaata ttgatttgc ggaagcaag 
      aatttcgat tttgacgag ctcgagagt tcgaccgag 
      tacgatggc ggttacacc agcgaaagc caaatcatc 
      aaagacttc tggtcaata gtccacgct ctctccctc 
      gaagacaaa agaaaacta ctccagttc acaacagga 
      tcggatcga gtcccgatc ggaggtttg agtcgactc 
      aaattggta attgcaaga aacggtccc gattcggat 
      cgattacca acggcacac acttgcttt aatgtttta 
      ttattgccg gaatattcg tcgaaagag aaacttaaa 
      gatagattg atcaaagcc ataagttac tcgaaaggc 
      tttggcatg ttgtaa
    mRNA sequence
    • >TC011206 RA
      aatcacaat ttttgacat caaagtctc gacaccagg 
      tgcgctcga aagtcccca actcgaaat gacagagca 
      atcattagc aatgtcgta aaatcttcg agcctcaag 
      taaaaacaa acaaaatat ccgttttaa attttacga 
      caaagtatt tcaaagttc tctcgccgt tcctcaatg 
      tgggacccc atgtgctaa agtccttgc gccaccttt 
      tgtatcttt tatttttct agtgtttta gcgtttatt 
      tatttacac aagaaaatt ctatgagct cttcagacc 
      caccaactg accaagacg acgcttcaa ggataaacc 
      aagaggctt cgtctagca ccaacaacg aaacctcta 
      cccctgaca ataaggagg acgagatga aacgtgctg 
      ctgcccgca agctgattg aacgatatt tctaccaat 
      tgaccgacg gttgtggga accccgcct gcaagaaca 
      agtactgcg cctccagcg gacagctca agccactca 
      cgccagacg aggcggctg cgcaagcca ttcagctct 
      tctctcaag aagcgattt tgtgcgaga gacacccta 
      acaaaatac agaggacta acaataaaa tggataatg 
      accacaaaa tgctttcgt cggattccg acgagtcaa 
      tcaatcgca aaacgggcc tcacaccaa ataacgaga 
      ttttatctg gcaatgaaa gctatatca agctcaaca 
      ctaccaaca actcgacta gttatttga cggaacaaa 
      tgctttacg acataatcg acgagtgcg aatcgaccg 
      aatgctact tgagtctaa tccgaaaac tgggcgaag 
      tcttcagca gcatagaaa acctgtctc ggagcttcc 
      aacagagcg acaagagca gccccagtg aggcaaacc 
      tggacgaaa cgggcctca aaaaggagg acgtgcgct 
      cgctcgagg gcgagaaag acaaggacg aagactcct 
      cggcggaga cccccgatg ggggcgtcc tccccgtcg 
      acctcccct cggtccgac gcgcttact cggccctct 
      tcaagctga aaacgtcga ttttcgagc actctctgg 
      tcaacgcgt tggttactt tggccggca acctccaga 
      tggagctgc cgacgcgga aagagcgcg gcaaccagg 
      acgatgtgg tcaatgttt tgttgattg tttttgaaa 
      ttcccgcgc tcggttcgg gggatttcc tcgaaacgg 
      cgttgccgg cgatctgtc gggcttccg aatggctgc 
      cggtggaag tccaagcga agttggcgc ggatttggt 
      cggggacgg gacgctcga gtcttagga acattttgg 
      agaatttgc aacagttgg tgacactga gggtgatcg 
      tgacgcagt ttcataggg atttttatg ttcaggatg 
      agaatgtta tcacgtcgg ctacgaaat taatgaaga 
      ttctttttt atgcgaata ttttggcgg gggttttgg 
      aatcgcctg atttgcgga atgaagatt tgtcaatgt 
      ccatggatg attcgtatg ttgctatca aattgaata 
      aaacgcaac caccggttg atcctttgg ctgccgagt 
      tgggagtgc acgttttgg actgtcgca aaccttatt 
      taccatttt ctgaattct ataatgagc ctttgagtg 
      atggtgtcg agatggatc gagactttg caaattata 
      agagcgaac ttggaaagt ttagtttta tgaattatc 
      cctttattt tgacgccgg cgaccaaaa tgatgggtt 
      tatatttcg ataaccgga ttcggatgt attcagaac 
      gacggatta gtattttac agtcggtta caggccaac 
      cttccaacc catatttga ggctgaagg ttaggaggg 
      atcacataa ttgacgatg ccttggttg agctggaga 
      tgatatcta tggagaacc ccaacgatt tgaagaagc 
      agttggtgg tggagttcg aaggcgagc aaggaattg 
      acgaaggcg gcgtgtcaa aggaattct ttcagttag 
      tgatcgaag aaattttca atcccgact atgcgatgt 
      ttacgagtc agagtgaga caggcacag tttggttca 
      atcccacta gtttcgaaa gtgacgctc aattcacat 
      taattggaa tagtgttag gacttgcca tttataata 
      atgttatct tggccgtga atttcccta tggtacttt 
      acaggaaac tgttaggta aaaggggct catttgaag 
      acttacaag actggaatc ttactttat ataatagtt 
      taaaacaat tactggaat ataacgagc ctgacgttg 
      aggaagttt ttatgcaaa cgttccgaa ttagctacc 
      aagatgtgt tcggttcta ttataaact acgacttaa 
      aagatcgcg gcgacgaaa ttaacgtaa cacaagaaa 
      ataaatatg aatttgtag atttgtacg ccgatttct 
      tactaaata aatcagtag agaaacagt ttcgtgcct 
      tttacaaag ggttccaaa tggtcgtag acgagtcac 
      cgctggaat tattgttcc gtcccgaag aaattgaaa 
      tattgattt gcggaagca agaatttcg attttgacg 
      agctcgaga gttcgaccg agtacgatg gcggttaca 
      ccagcgaaa gccaaatca tcaaagact tctggtcaa 
      tagtccacg ctctctccc tcgaagaca aaagaaaac 
      tactccagt tcacaacag gatcggatc gagtcccga 
      tcggaggtt tgagtcgac tcaaattgg taattgcaa 
      gaaacggtc ccgattcgg atcgattac caacggcac 
      acacttgct ttaatgttt tattattgc cggaatatt 
      cgtcgaaag agaaactta aagatagat tgatcaaag 
      ccataagtt actcgaaag gctttggca tgttgtaaa 
      ttgagtttt gcttgtgaa tactttctt ttcgaatta 
      gaaaatacg atttgagtt gtttactaa caaaagttg 
      tattttgtt tgagtaact taatataat tatttaaaa 
      aaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY