Status
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Gene information
General information
TC011193 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 19303316..19307220
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011193
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04984

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 9 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011193 RA
      MSGDSGNPI YTSEKTGND DSGLGTEDK PFQTILRAM 
      RHAGAEPFP VIYVDGKEG TKYEPASKS QLKKIQKIW 
      LRETYKQSS VAKREEEDA AKREKNLEE ATKITLTED 
      KSLPAAKLI KIAKGKEFR DQRVKIYGW VHRLRRQGK 
      NLMFITLRD GTGFLQSVL TDKLCQTYN ALVLSTESS 
      VLLYGSLKK VPEGKTAPG GHELAVDYW ELVGLAPPG 
      GADSILNEQ ALPDVQAEN RHIMIRGEN TSKILRMRS 
      VLLQAFRDH YLDRGYCEV TPPSIVQTQ CEGGSTLFK 
      MDYFGEEAY LTQSSQLYL ETCLPSMGD VFCIAESFR 
      AEQSRTRRH LAEYTHVEA ECPFISFND LLDRLEDLI 
      CDVVDRVLK SPYGDIVHE LNPDFQVPT RPFLRMNYS 
      DAIDYLKKN NITKEDGSF YEFGEDIPE MPERKMTDQ 
      INKPIMLCR FPANIKSFY MSKCPEDAR LTESVDVLL 
      PNVGEIVGG SMRIHNLEE LMDGYKREG IDPTPYFWY 
      TDQRKYGTC PHGGYGLGL ERFVCWLLN RYHIREVCL 
      YPRYLHHCK P
    CDS sequence
    • >TC011193 RA
      atgtctggt gacagcgga aatccaatt tacacatct 
      gagaaaacc ggaaacgac gattccggt ctcggcacc 
      gaagacaag ccctttcaa acgatcctt cgagctatg 
      cgtcacgct ggggccgaa ccatttccc gtaatctac 
      gttgacggc aaagaaggc accaaatat gaacccgcc 
      tccaaatca caattgaaa aaaatccaa aaaatatgg 
      ctccgagag acttacaaa caatcaagt gtcgccaaa 
      cgcgaggaa gaagacgcc gcaaaacgc gaaaaaaat 
      ctcgaagaa gcgacaaaa atcacacta actgaagac 
      aaatccttg ccggcggcc aaattgatc aaaatcgcc 
      aaaggcaaa gagttcaga gatcaaaga gtgaagatt 
      tacggatgg gtgcacagg ctgcgccga caggggaaa 
      aacctgatg tttatcacg ttgagggat gggacggga 
      tttttgcaa agtgtactc actgataag ctttgtcaa 
      acttataac gcacttgtt ttatcgacc gagtcgtcg 
      gttttgctt tatggtagt ttgaaaaaa gtaccagag 
      ggtaaaaca gcgccaggt gggcatgag ttggctgtg 
      gactactgg gaattggtg gggttagcg ccacctggc 
      ggcgccgac tcgatacta aacgagcag gctttgcca 
      gacgttcag gccgaaaat cgccacatt atgataagg 
      ggcgaaaac acctcaaaa attttgcga atgcgatca 
      gtccttcta caggcgttc agggaccac tatttggat 
      cgcggttac tgtgaagta acaccgcca tctatcgtc 
      caaactcaa tgtgaaggg ggctcgact ttattcaaa 
      atggactat tttggtgag gaggcctac ttgacccag 
      agctcgcag ctgtacttg gagacgtgt ttgccctcc 
      atgggggac gttttttgc attgcggag agttttagg 
      gctgagcag agccgcact aggcggcat ttggccgaa 
      tacacacat gtggaggcc gaatgccct ttcatttcc 
      ttcaatgat ttgttggac cgtctcgag gaccttatt 
      tgtgatgtt gttgaccgc gttttgaag tctccttat 
      ggagatatt gtccatgaa ctgaaccca gactttcaa 
      gtaccgaca cgaccattc ctccgaatg aactattcc 
      gatgccatc gactacctc aagaagaat aatatcaca 
      aaggaggat ggcagtttt tacgaattt ggggaggat 
      attcctgaa atgcctgaa cgaaaaatg accgaccag 
      atcaacaaa cccattatg ttgtgccgt tttccggca 
      aatattaaa tcgttttac atgtccaaa tgcccagag 
      gatgcgcgt ttgactgaa agtgtggac gttttgttg 
      ccgaatgtt ggcgaaatt gttggaggg tcgatgagg 
      atacacaat ttggaggaa cttatggat gggtataaa 
      cgggaaggc atagacccg accccgtac ttctggtac 
      acggaccag agaaagtac gggacgtgc ccccatggg 
      ggctacggc ctcggattg gagcggttt gtctgttgg 
      ttgttgaac agataccac attagagag gtgtgtctc 
      tacccccgg tacttgcac cactgtaaa ccctaa
    mRNA sequence
    • >TC011193 RA
      tgcacaata gatgtgaat taaagaaca aaattgcat 
      cagaattta attgtgaat taactgaaa caggtgttt 
      agtcaatat cttgagatt ttagtgaac tcaaaatgt 
      ctggtgaca gcggaaatc caatttaca catctgaga 
      aaaccggaa acgacgatt ccggtctcg gcaccgaag 
      acaagccct ttcaaacga tccttcgag ctatgcgtc 
      acgctgggg ccgaaccat ttcccgtaa tctacgttg 
      acggcaaag aaggcacca aatatgaac ccgcctcca 
      aatcacaat tgaaaaaaa tccaaaaaa tatggctcc 
      gagagactt acaaacaat caagtgtcg ccaaacgcg 
      aggaagaag acgccgcaa aacgcgaaa aaaatctcg 
      aagaagcga caaaaatca cactaactg aagacaaat 
      ccttgccgg cggccaaat tgatcaaaa tcgccaaag 
      gcaaagagt tcagagatc aaagagtga agatttacg 
      gatgggtgc acaggctgc gccgacagg ggaaaaacc 
      tgatgttta tcacgttga gggatggga cgggatttt 
      tgcaaagtg tactcactg ataagcttt gtcaaactt 
      ataacgcac ttgttttat cgaccgagt cgtcggttt 
      tgctttatg gtagtttga aaaaagtac cagagggta 
      aaacagcgc caggtgggc atgagttgg ctgtggact 
      actgggaat tggtggggt tagcgccac ctggcggcg 
      ccgactcga tactaaacg agcaggctt tgccagacg 
      ttcaggccg aaaatcgcc acattatga taaggggcg 
      aaaacacct caaaaattt tgcgaatgc gatcagtcc 
      ttctacagg cgttcaggg accactatt tggatcgcg 
      gttactgtg aagtaacac cgccatcta tcgtccaaa 
      ctcaatgtg aagggggct cgactttat tcaaaatgg 
      actattttg gtgaggagg cctacttga cccagagct 
      cgcagctgt acttggaga cgtgtttgc cctccatgg 
      gggacgttt tttgcattg cggagagtt ttagggctg 
      agcagagcc gcactaggc ggcatttgg ccgaataca 
      cacatgtgg aggccgaat gccctttca tttccttca 
      atgatttgt tggaccgtc tcgaggacc ttatttgtg 
      atgttgttg accgcgttt tgaagtctc cttatggag 
      atattgtcc atgaactga acccagact ttcaagtac 
      cgacacgac cattcctcc gaatgaact attccgatg 
      ccatcgact acctcaaga agaataata tcacaaagg 
      aggatggca gtttttacg aatttgggg aggatattc 
      ctgaaatgc ctgaacgaa aaatgaccg accagatca 
      acaaaccca ttatgttgt gccgttttc cggcaaata 
      ttaaatcgt tttacatgt ccaaatgcc cagaggatg 
      cgcgtttga ctgaaagtg tggacgttt tgttgccga 
      atgttggcg aaattgttg gagggtcga tgaggatac 
      acaatttgg aggaactta tggatgggt ataaacggg 
      aaggcatag acccgaccc cgtacttct ggtacacgg 
      accagagaa agtacggga cgtgccccc atgggggct 
      acggcctcg gattggagc ggtttgtct gttggttgt 
      tgaacagat accacatta gagaggtgt gtctctacc 
      cccggtact tgcaccact gtaaaccct aagcatata 
      tttacattt aacatgcat ttaaattgt cctaagctt 
      gtttaagtg aaataaagg ccaataaaa acgaaaatt 
      cctt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY