Status
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Gene information
General information
TC011192 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 19296066..19298413
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011192
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04983

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    legs distal decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011192 RA
      MASAASLIK YTKLFINNE FVDSVSKKT FPTLNPTNG 
      AKIIDISEG DKEDIDKAV QAAKAAFSR GAPWRKMDA 
      STRGKLIIK LADLIERDI NTLAKLETL DNGKPLSDS 
      TLDIQCSID VLRYYGGYC DKIHGNTIP ADGDCLSLT 
      RKEPVGVVG QIIPWNYPI MMQIWKWGP ALAAGCTMV 
      LKPAEQTPI TALALAALV KEAGFPPGV VNVVPGYGP 
      TAGAALALH KDVNKVAFT GSTIVGKKI MEYSAQSNL 
      KRVSLELGG KSPLVVFND ADLDEAVEI AHNAIFGNH 
      GQNCCAGSR TFVQSGIYD AFVQKAVQK AKLRKVGDP 
      FDPNVQQGP QIDKPSLDK ILRLIDSGK KEGAKLEIG 
      GSRIGTEGY FVEPTVFSN VTDAMSIAK EEIFGPVQS 
      ILKFETLEE VIERANDTY YGLAAGVIT KNIDTALTF 
      AQAVQAGSV WVNCYDYIT PQTPFGGYK MSGIGREMG 
      AESLDAYVE TKTISIKLP TKV
    CDS sequence
    • >TC011192 RA
      atggcttcc gcagcgtct ttaattaaa tacacaaag 
      ttgtttata aacaacgaa tttgtggac tctgtgagt 
      aaaaaaacc ttcccaact ctgaacccc acaaatggg 
      gccaaaatc atcgacatt tccgagggg gacaaggaa 
      gacattgac aaggcggtc caagcggct aaagcagcc 
      ttttccaga ggcgcccca tggcgcaaa atggatgcc 
      tcaacccga ggcaaactc ataatcaag ctcgccgac 
      ttgatcgaa cgcgatatt aacacgctc gcaaagctg 
      gaaaccctt gataacggc aaaccctta tcagactca 
      acgctcgat atccagtgc agcatcgac gtcttgcgc 
      tactatggg ggctattgc gacaaaatc cacggtaac 
      acaatcccc gcagacggc gattgtctc tccctcacc 
      cgcaaggag ccggtcggc gtcgtgggc cagatcatc 
      ccctggaac taccccatc atgatgcag atctggaaa 
      tggggcccc gccctggcc gctggctgc accatggtg 
      ctgaagccg gccgagcag acccccatc acggccctc 
      gcgttggcg gccctggtc aaagaggcg gggtttccc 
      ccgggggtg gtgaacgtg gtccccggg tacgggccc 
      acggcgggg gcggcccta gccctccac aaagacgtc 
      aataaagtg gctttcacc ggatccaca attgtcggc 
      aaaaaaatc atggagtat agcgcgcag tcgaacttg 
      aaacgtgtg agtctcgag ctggggggc aagagcccc 
      cttgtggtc ttcaatgac gctgatttg gatgaggcg 
      gtggaaatc gcccataat gcgattttt ggcaaccac 
      ggccagaat tgttgtgcc ggttcccgt acttttgtt 
      caatctgga atttacgat gcttttgta caaaaagcg 
      gtccaaaag gccaagctg cgtaaagtt ggcgatcca 
      tttgacccc aatgtgcaa caagggcct caaattgat 
      aagccatcg cttgataaa atcttgcgt ttgattgat 
      tcggggaag aaggaaggg gcgaaattg gaaatcggg 
      ggttcccgg atcgggact gaggggtat ttcgtcgaa 
      ccgactgtc ttttcgaat gttactgat gctatgagc 
      attgccaaa gaagagatt tttggccct gtgcaaagc 
      attttgaaa tttgagacc ctggaggag gttattgaa 
      agggccaat gacacgtat tacggtttg gcggctggg 
      gttatcacc aaaaatatt gatacggcc ctgacattc 
      gcccaagcg gtccaagcc ggttctgtt tgggtgaac 
      tgttacgac tacatcacg ccacaaacg ccgttcggg 
      ggctacaaa atgtccgga atcgggcgt gaaatgggg 
      gccgagtca ctagacgcg tacgtggaa accaaaaca 
      atttccatc aaactgcca actaaggtc taa
    mRNA sequence
    • >TC011192 RA
      gagatcaga ggtcaaagc agtgattag atatctatc 
      taattcctt aagaaaaca acaattaca taaaaaatt 
      gccacgttg aaacatggc ttccgcagc gtctttaat 
      taaatacac aaagttgtt tataaacaa cgaatttgt 
      ggactctgt gagtaaaaa aaccttccc aactctgaa 
      ccccacaaa tggggccaa aatcatcga catttccga 
      gggggacaa ggaagacat tgacaaggc ggtccaagc 
      ggctaaagc agccttttc cagaggcgc cccatggcg 
      caaaatgga tgcctcaac ccgaggcaa actcataat 
      caagctcgc cgacttgat cgaacgcga tattaacac 
      gctcgcaaa gctggaaac ccttgataa cggcaaacc 
      cttatcaga ctcaacgct cgatatcca gtgcagcat 
      cgacgtctt gcgctacta tgggggcta ttgcgacaa 
      aatccacgg taacacaat ccccgcaga cggcgattg 
      tctctccct cacccgcaa ggagccggt cggcgtcgt 
      gggccagat catcccctg gaactaccc catcatgat 
      gcagatctg gaaatgggg ccccgccct ggccgctgg 
      ctgcaccat ggtgctgaa gccggccga gcagacccc 
      catcacggc cctcgcgtt ggcggccct ggtcaaaga 
      ggcggggtt tcccccggg ggtggtgaa cgtggtccc 
      cgggtacgg gcccacggc gggggcggc cctagccct 
      ccacaaaga cgtcaataa agtggcttt caccggatc 
      cacaattgt cggcaaaaa aatcatgga gtatagcgc 
      gcagtcgaa cttgaaacg tgtgagtct cgagctggg 
      gggcaagag cccccttgt ggtcttcaa tgacgctga 
      tttggatga ggcggtgga aatcgccca taatgcgat 
      ttttggcaa ccacggcca gaattgttg tgccggttc 
      ccgtacttt tgttcaatc tggaattta cgatgcttt 
      tgtacaaaa agcggtcca aaaggccaa gctgcgtaa 
      agttggcga tccatttga ccccaatgt gcaacaagg 
      gcctcaaat tgataagcc atcgcttga taaaatctt 
      gcgtttgat tgattcggg gaagaagga aggggcgaa 
      attggaaat cgggggttc ccggatcgg gactgaggg 
      gtatttcgt cgaaccgac tgtcttttc gaatgttac 
      tgatgctat gagcattgc caaagaaga gatttttgg 
      ccctgtgca aagcatttt gaaatttga gaccctgga 
      ggaggttat tgaaagggc caatgacac gtattacgg 
      tttggcggc tggggttat caccaaaaa tattgatac 
      ggccctgac attcgccca agcggtcca agccggttc 
      tgtttgggt gaactgtta cgactacat cacgccaca 
      aacgccgtt cgggggcta caaaatgtc cggaatcgg 
      gcgtgaaat gggggccga gtcactaga cgcgtacgt 
      ggaaaccaa aacaatttc catcaaact gccaactaa 
      ggtctaaaa cttttcatt ttcgtaaaa agca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY