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Gene information
General information
TC011190 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 19278516..19284175
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011190
    iBeetle screen iB_04982

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011190 RA
      MTERYSFAV DTSVWTQDN IDTVHSTLT DTINESYAT 
      AVGDSPSRY LSVLDTSGE MSLENLNIN LTNPKLEVE 
      EPVENFISE EIFEQPQEE SNDQQVVVF SIEGSDELF 
      GIQVAQDEE GNLQKYQFQ FRKNEEGQL EAIPETIQL 
      LPMEEEQEG GEQGEDNVQ LFLQEEEHN EHILPDNTL 
      VHEQEDDSM LYENEEPVD IKPAILSLQ NIKQESQSE 
      YEIAEPISE ESPEQQVDY ETPPEPTQE EGTILSDTK 
      VFEHVEVRP DMEITHHEP PEEIEDFNN ENVNDSDEN 
      DYQIVEGVH ITGDVEPNE MDKQNTIPS TNILKHVVK 
      STKDAHKQK KDNKLFFYV VQHPEKENV DSLSLEMSN 
      LLCRPKVNP RSILKSSHA MFQAKNEME NVEQEDERF 
      NKRFARCKE AMQARIFNN FINKTTIPQ APVRQSRLP 
      RKQKIKPLE RMDEEIVVQ EVMVSSTGF VDTASGGVN 
      KENFEVTEY VELTDSDDD YDPKKVIRK KKKHRRRKI 
      EITISDDSD ASHDSVIEL DETDDEEPK SPDAPKRRR 
      GRPPKKSRV ESNDIASKP EIPCPKCEK TFPSQNSLK 
      THLQHHNLQ SSIKNALPE SKYKCDDCA ISFKNSILL 
      KNHKCLKKF TCPVCKKKF PDMSSLTGH KRVHAKEQL 
      VKSTAVVRV SPKKMKPVL PKSKSPQNK CKTCGKICS 
      SEQNLNVHM KTHKEYVCS TCSATFVSQ FMLEKHVRE 
      NCVKFKVPQ VTRRSMRIK NASSAEQLS PKSPKRSPM 
      KGRERLTMS SLKLKCDKC NDQFGTFKA LFRHKVVKH 
      NMETPDKEA LEKEQPEEK THEGVPVAK GLQNVWTGV 
      NLKLDF
    CDS sequence
    • >TC011190 RA
      atgactgaa cgctactcg tttgctgtt gatacgagc 
      gtctggacg caagacaac atcgatacg gtgcactcg 
      accctcaca gataccatt aacgagagc tatgcaacg 
      gccgttgga gactcccca agtcggtat ttgtcggtc 
      ttggacacg agcggcgaa atgtcgctg gaaaacctc 
      aacatcaac ctgacaaac cccaaactg gaggtggag 
      gaaccggtg gagaatttc atttcggag gagattttt 
      gagcagccc caggaggag tcaaacgac caacaagtc 
      gtagtcttt tcaattgag ggctctgac gagctgttc 
      ggcatccag gtggcgcag gacgaggag gggaatttg 
      cagaaatac caattccag tttcggaaa aatgaggag 
      gggcaactt gaggcaatc cctgagacc atccaactg 
      ctcccgatg gaggaggag caggagggc ggcgaacaa 
      ggggaggac aacgtgcag ttgttcctc caagaggag 
      gaacacaac gaacacatt ttaccagac aatactctg 
      gtgcatgag caagaggac gactccatg ttgtacgaa 
      aatgaggaa cccgttgat attaaaccc gccatcttg 
      tcgcttcaa aatatcaaa caagagagc caatcagag 
      tacgagatc gcggaacca atcagtgaa gagtctcct 
      gagcaacaa gtcgactat gaaacacct ccagagccc 
      actcaggaa gaaggaacg attttatcc gataccaaa 
      gttttcgaa cacgtggaa gtacgacct gacatggaa 
      ataacccac cacgaacca ccggaagaa atcgaagat 
      ttcaacaat gaaaacgtg aacgattcg gacgaaaac 
      gattaccag atcgtagaa ggcgtgcac ataaccggt 
      gacgttgaa ccaaatgaa atggacaaa caaaacacc 
      atccccagc acaaatatc ctaaaacac gtcgtgaaa 
      tcgaccaag gacgcacat aaacaaaaa aaagacaac 
      aagttgttt ttctacgtt gtccaacac ccggaaaag 
      gaaaacgtc gattctttg tcgctagaa atgtcgaat 
      ttgctgtgc cggcctaag gtcaatccg aggtcaatt 
      ttgaagagt tcgcatgcg atgtttcaa gcgaaaaat 
      gaaatggag aatgtcgag caggaggat gagagattt 
      aataagcga tttgcgagg tgtaaggag gcgatgcaa 
      gcgagaatt tttaataat tttattaat aaaacaaca 
      ataccacaa gcgcccgtc cggcaatca cgtctgccc 
      agaaaacag aaaattaaa cctttggaa aggatggat 
      gaggagatt gtggtccag gaagtgatg gtgtcgtcg 
      actggattc gtagacacg gcttctgga ggtgtcaat 
      aaagagaac ttcgaagtg accgaatac gtcgaactg 
      acggattcg gacgacgac tacgacccg aaaaaagtc 
      atccgaaaa aagaagaaa caccgaagg aggaaaatc 
      gagattacg ataagcgac gacagtgac gcttcgcac 
      gacagcgtc atcgaattg gacgaaacc gacgacgaa 
      gaaccgaaa agtcccgac gcccccaag cgacgacgt 
      ggtcgtccc cccaaaaaa tcccgtgtg gaatcgaac 
      gacatagct tcaaaaccc gaaattccc tgtccaaaa 
      tgcgaaaaa accttccct agtcaaaac agcctcaag 
      acacacctc caacaccat aatcttcaa agttcgatc 
      aaaaacgcg ttacctgaa tcgaagtac aaatgcgac 
      gattgcgcc atctctttc aaaaacagt atcctgttg 
      aagaaccac aagtgtttg aaaaagttc acttgtccg 
      gtttgtaaa aagaaattt cctgatatg tcgagtctg 
      accggtcat aagcgagtc catgcgaaa gagcaactt 
      gttaaaagt acagccgtt gtgcgggtt tcccccaaa 
      aaaatgaaa ccagtgttg ccaaagtcg aaaagtccc 
      cagaataaa tgtaaaacc tgcgggaaa atttgctcg 
      tcggagcaa aacttgaac gttcacatg aaaacgcac 
      aaggagtat gtttgtagc acttgttcg gcgactttc 
      gtttcgcag tttatgttg gagaaacac gttagggaa 
      aattgcgtc aagttcaag gtgcctcaa gttacgagg 
      aggtcgatg aggatcaag aatgcctcg agtgcggag 
      caactttcg ccgaaaagc cccaaaaga agccctatg 
      aagggcagg gagaggtta acgatgagt tcactcaaa 
      ctcaaatgt gacaaatgt aatgaccag tttgggact 
      tttaaagca ctatttaga cacaaagtg gtgaagcac 
      aacatggag acgccggat aaggaggcc ttggagaag 
      gagcagcct gaggaaaag acacacgag ggggttcct 
      gtagcgaag ggtttgcag aatgtgtgg acgggtgtc 
      aacttaaaa ttggatttt taa
    mRNA sequence
    • >TC011190 RA
      tccgttggc tttggacgc ctagttcga cctaaaaat 
      agttgtcaa gtgtcaaca agcaagaaa cggtttttt 
      aaagggaaa atgtgttaa agaaagtaa aaccacgct 
      tcctggctg gttttatag tgaaaaagt gtgaacttt 
      attgaaaat tgcgccaat tcgtgtttg tttcgtact 
      agaaattgg aaatcaagt cgaacccca agtcgtaga 
      tttgcatgg gatttaatt atttatcaa agacgagaa 
      atgactgaa cgctactcg tttgctgtt gatacgagc 
      gtctggacg caagacaac atcgatacg gtgcactcg 
      accctcaca gataccatt aacgagagc tatgcaacg 
      gccgttgga gactcccca agtcggtat ttgtcggtc 
      ttggacacg agcggcgaa atgtcgctg gaaaacctc 
      aacatcaac ctgacaaac cccaaactg gaggtggag 
      gaaccggtg gagaatttc atttcggag gagattttt 
      gagcagccc caggaggag tcaaacgac caacaagtc 
      gtagtcttt tcaattgag ggctctgac gagctgttc 
      ggcatccag gtggcgcag gacgaggag gggaatttg 
      cagaaatac caattccag tttcggaaa aatgaggag 
      gggcaactt gaggcaatc cctgagacc atccaactg 
      ctcccgatg gaggaggag caggagggc ggcgaacaa 
      ggggaggac aacgtgcag ttgttcctc caagaggag 
      gaacacaac gaacacatt ttaccagac aatactctg 
      gtgcatgag caagaggac gactccatg ttgtacgaa 
      aatgaggaa cccgttgat attaaaccc gccatcttg 
      tcgcttcaa aatatcaaa caagagagc caatcagag 
      tacgagatc gcggaacca atcagtgaa gagtctcct 
      gagcaacaa gtcgactat gaaacacct ccagagccc 
      actcaggaa gaaggaacg attttatcc gataccaaa 
      gttttcgaa cacgtggaa gtacgacct gacatggaa 
      ataacccac cacgaacca ccggaagaa atcgaagat 
      ttcaacaat gaaaacgtg aacgattcg gacgaaaac 
      gattaccag atcgtagaa ggcgtgcac ataaccggt 
      gacgttgaa ccaaatgaa atggacaaa caaaacacc 
      atccccagc acaaatatc ctaaaacac gtcgtgaaa 
      tcgaccaag gacgcacat aaacaaaaa aaagacaac 
      aagttgttt ttctacgtt gtccaacac ccggaaaag 
      gaaaacgtc gattctttg tcgctagaa atgtcgaat 
      ttgctgtgc cggcctaag gtcaatccg aggtcaatt 
      ttgaagagt tcgcatgcg atgtttcaa gcgaaaaat 
      gaaatggag aatgtcgag caggaggat gagagattt 
      aataagcga tttgcgagg tgtaaggag gcgatgcaa 
      gcgagaatt tttaataat tttattaat aaaacaaca 
      ataccacaa gcgcccgtc cggcaatca cgtctgccc 
      agaaaacag aaaattaaa cctttggaa aggatggat 
      gaggagatt gtggtccag gaagtgatg gtgtcgtcg 
      actggattc gtagacacg gcttctgga ggtgtcaat 
      aaagagaac ttcgaagtg accgaatac gtcgaactg 
      acggattcg gacgacgac tacgacccg aaaaaagtc 
      atccgaaaa aagaagaaa caccgaagg aggaaaatc 
      gagattacg ataagcgac gacagtgac gcttcgcac 
      gacagcgtc atcgaattg gacgaaacc gacgacgaa 
      gaaccgaaa agtcccgac gcccccaag cgacgacgt 
      ggtcgtccc cccaaaaaa tcccgtgtg gaatcgaac 
      gacatagct tcaaaaccc gaaattccc tgtccaaaa 
      tgcgaaaaa accttccct agtcaaaac agcctcaag 
      acacacctc caacaccat aatcttcaa agttcgatc 
      aaaaacgcg ttacctgaa tcgaagtac aaatgcgac 
      gattgcgcc atctctttc aaaaacagt atcctgttg 
      aagaaccac aagtgtttg aaaaagttc acttgtccg 
      gtttgtaaa aagaaattt cctgatatg tcgagtctg 
      accggtcat aagcgagtc catgcgaaa gagcaactt 
      gttaaaagt acagccgtt gtgcgggtt tcccccaaa 
      aaaatgaaa ccagtgttg ccaaagtcg aaaagtccc 
      cagaataaa tgtaaaacc tgcgggaaa atttgctcg 
      tcggagcaa aacttgaac gttcacatg aaaacgcac 
      aaggagtat gtttgtagc acttgttcg gcgactttc 
      gtttcgcag tttatgttg gagaaacac gttagggaa 
      aattgcgtc aagttcaag gtgcctcaa gttacgagg 
      aggtcgatg aggatcaag aatgcctcg agtgcggag 
      caactttcg ccgaaaagc cccaaaaga agccctatg 
      aagggcagg gagaggtta acgatgagt tcactcaaa 
      ctcaaatgt gacaaatgt aatgaccag tttgggact 
      tttaaagca ctatttaga cacaaagtg gtgaagcac 
      aacatggag acgccggat aaggaggcc ttggagaag 
      gagcagcct gaggaaaag acacacgag ggggttcct 
      gtagcgaag ggtttgcag aatgtgtgg acgggtgtc 
      aacttaaaa ttggatttt taagattaa ggctatgta 
      gatttgtaa ataggcttc ttatgtaca tttagcata 
      ttgtgtatt ttaataaat ttttcaacc gtaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY