Status
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Gene information
General information
TC011190 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 19278516..19284175
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011190
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04982

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011190 RA
      MTERYSFAV DTSVWTQDN IDTVHSTLT DTINESYAT 
AVGDSPSRY LSVLDTSGE MSLENLNIN LTNPKLEVE 
EPVENFISE EIFEQPQEE SNDQQVVVF SIEGSDELF 
GIQVAQDEE GNLQKYQFQ FRKNEEGQL EAIPETIQL 
LPMEEEQEG GEQGEDNVQ LFLQEEEHN EHILPDNTL 
VHEQEDDSM LYENEEPVD IKPAILSLQ NIKQESQSE 
YEIAEPISE ESPEQQVDY ETPPEPTQE EGTILSDTK 
VFEHVEVRP DMEITHHEP PEEIEDFNN ENVNDSDEN 
DYQIVEGVH ITGDVEPNE MDKQNTIPS TNILKHVVK 
STKDAHKQK KDNKLFFYV VQHPEKENV DSLSLEMSN 
LLCRPKVNP RSILKSSHA MFQAKNEME NVEQEDERF 
NKRFARCKE AMQARIFNN FINKTTIPQ APVRQSRLP 
RKQKIKPLE RMDEEIVVQ EVMVSSTGF VDTASGGVN 
KENFEVTEY VELTDSDDD YDPKKVIRK KKKHRRRKI 
EITISDDSD ASHDSVIEL DETDDEEPK SPDAPKRRR 
GRPPKKSRV ESNDIASKP EIPCPKCEK TFPSQNSLK 
THLQHHNLQ SSIKNALPE SKYKCDDCA ISFKNSILL 
KNHKCLKKF TCPVCKKKF PDMSSLTGH KRVHAKEQL 
VKSTAVVRV SPKKMKPVL PKSKSPQNK CKTCGKICS 
SEQNLNVHM KTHKEYVCS TCSATFVSQ FMLEKHVRE 
NCVKFKVPQ VTRRSMRIK NASSAEQLS PKSPKRSPM 
KGRERLTMS SLKLKCDKC NDQFGTFKA LFRHKVVKH 
NMETPDKEA LEKEQPEEK THEGVPVAK GLQNVWTGV 
NLKLDF
    CDS sequence
    • >TC011190 RA
      atgactgaa cgctactcg tttgctgtt gatacgagc 
gtctggacg caagacaac atcgatacg gtgcactcg 
accctcaca gataccatt aacgagagc tatgcaacg 
gccgttgga gactcccca agtcggtat ttgtcggtc 
ttggacacg agcggcgaa atgtcgctg gaaaacctc 
aacatcaac ctgacaaac cccaaactg gaggtggag 
gaaccggtg gagaatttc atttcggag gagattttt 
gagcagccc caggaggag tcaaacgac caacaagtc 
gtagtcttt tcaattgag ggctctgac gagctgttc 
ggcatccag gtggcgcag gacgaggag gggaatttg 
cagaaatac caattccag tttcggaaa aatgaggag 
gggcaactt gaggcaatc cctgagacc atccaactg 
ctcccgatg gaggaggag caggagggc ggcgaacaa 
ggggaggac aacgtgcag ttgttcctc caagaggag 
gaacacaac gaacacatt ttaccagac aatactctg 
gtgcatgag caagaggac gactccatg ttgtacgaa 
aatgaggaa cccgttgat attaaaccc gccatcttg 
tcgcttcaa aatatcaaa caagagagc caatcagag 
tacgagatc gcggaacca atcagtgaa gagtctcct 
gagcaacaa gtcgactat gaaacacct ccagagccc 
actcaggaa gaaggaacg attttatcc gataccaaa 
gttttcgaa cacgtggaa gtacgacct gacatggaa 
ataacccac cacgaacca ccggaagaa atcgaagat 
ttcaacaat gaaaacgtg aacgattcg gacgaaaac 
gattaccag atcgtagaa ggcgtgcac ataaccggt 
gacgttgaa ccaaatgaa atggacaaa caaaacacc 
atccccagc acaaatatc ctaaaacac gtcgtgaaa 
tcgaccaag gacgcacat aaacaaaaa aaagacaac 
aagttgttt ttctacgtt gtccaacac ccggaaaag 
gaaaacgtc gattctttg tcgctagaa atgtcgaat 
ttgctgtgc cggcctaag gtcaatccg aggtcaatt 
ttgaagagt tcgcatgcg atgtttcaa gcgaaaaat 
gaaatggag aatgtcgag caggaggat gagagattt 
aataagcga tttgcgagg tgtaaggag gcgatgcaa 
gcgagaatt tttaataat tttattaat aaaacaaca 
ataccacaa gcgcccgtc cggcaatca cgtctgccc 
agaaaacag aaaattaaa cctttggaa aggatggat 
gaggagatt gtggtccag gaagtgatg gtgtcgtcg 
actggattc gtagacacg gcttctgga ggtgtcaat 
aaagagaac ttcgaagtg accgaatac gtcgaactg 
acggattcg gacgacgac tacgacccg aaaaaagtc 
atccgaaaa aagaagaaa caccgaagg aggaaaatc 
gagattacg ataagcgac gacagtgac gcttcgcac 
gacagcgtc atcgaattg gacgaaacc gacgacgaa 
gaaccgaaa agtcccgac gcccccaag cgacgacgt 
ggtcgtccc cccaaaaaa tcccgtgtg gaatcgaac 
gacatagct tcaaaaccc gaaattccc tgtccaaaa 
tgcgaaaaa accttccct agtcaaaac agcctcaag 
acacacctc caacaccat aatcttcaa agttcgatc 
aaaaacgcg ttacctgaa tcgaagtac aaatgcgac 
gattgcgcc atctctttc aaaaacagt atcctgttg 
aagaaccac aagtgtttg aaaaagttc acttgtccg 
gtttgtaaa aagaaattt cctgatatg tcgagtctg 
accggtcat aagcgagtc catgcgaaa gagcaactt 
gttaaaagt acagccgtt gtgcgggtt tcccccaaa 
aaaatgaaa ccagtgttg ccaaagtcg aaaagtccc 
cagaataaa tgtaaaacc tgcgggaaa atttgctcg 
tcggagcaa aacttgaac gttcacatg aaaacgcac 
aaggagtat gtttgtagc acttgttcg gcgactttc 
gtttcgcag tttatgttg gagaaacac gttagggaa 
aattgcgtc aagttcaag gtgcctcaa gttacgagg 
aggtcgatg aggatcaag aatgcctcg agtgcggag 
caactttcg ccgaaaagc cccaaaaga agccctatg 
aagggcagg gagaggtta acgatgagt tcactcaaa 
ctcaaatgt gacaaatgt aatgaccag tttgggact 
tttaaagca ctatttaga cacaaagtg gtgaagcac 
aacatggag acgccggat aaggaggcc ttggagaag 
gagcagcct gaggaaaag acacacgag ggggttcct 
gtagcgaag ggtttgcag aatgtgtgg acgggtgtc 
aacttaaaa ttggatttt taa
    mRNA sequence
    • >TC011190 RA
      tccgttggc tttggacgc ctagttcga cctaaaaat 
agttgtcaa gtgtcaaca agcaagaaa cggtttttt 
aaagggaaa atgtgttaa agaaagtaa aaccacgct 
tcctggctg gttttatag tgaaaaagt gtgaacttt 
attgaaaat tgcgccaat tcgtgtttg tttcgtact 
agaaattgg aaatcaagt cgaacccca agtcgtaga 
tttgcatgg gatttaatt atttatcaa agacgagaa 
atgactgaa cgctactcg tttgctgtt gatacgagc 
gtctggacg caagacaac atcgatacg gtgcactcg 
accctcaca gataccatt aacgagagc tatgcaacg 
gccgttgga gactcccca agtcggtat ttgtcggtc 
ttggacacg agcggcgaa atgtcgctg gaaaacctc 
aacatcaac ctgacaaac cccaaactg gaggtggag 
gaaccggtg gagaatttc atttcggag gagattttt 
gagcagccc caggaggag tcaaacgac caacaagtc 
gtagtcttt tcaattgag ggctctgac gagctgttc 
ggcatccag gtggcgcag gacgaggag gggaatttg 
cagaaatac caattccag tttcggaaa aatgaggag 
gggcaactt gaggcaatc cctgagacc atccaactg 
ctcccgatg gaggaggag caggagggc ggcgaacaa 
ggggaggac aacgtgcag ttgttcctc caagaggag 
gaacacaac gaacacatt ttaccagac aatactctg 
gtgcatgag caagaggac gactccatg ttgtacgaa 
aatgaggaa cccgttgat attaaaccc gccatcttg 
tcgcttcaa aatatcaaa caagagagc caatcagag 
tacgagatc gcggaacca atcagtgaa gagtctcct 
gagcaacaa gtcgactat gaaacacct ccagagccc 
actcaggaa gaaggaacg attttatcc gataccaaa 
gttttcgaa cacgtggaa gtacgacct gacatggaa 
ataacccac cacgaacca ccggaagaa atcgaagat 
ttcaacaat gaaaacgtg aacgattcg gacgaaaac 
gattaccag atcgtagaa ggcgtgcac ataaccggt 
gacgttgaa ccaaatgaa atggacaaa caaaacacc 
atccccagc acaaatatc ctaaaacac gtcgtgaaa 
tcgaccaag gacgcacat aaacaaaaa aaagacaac 
aagttgttt ttctacgtt gtccaacac ccggaaaag 
gaaaacgtc gattctttg tcgctagaa atgtcgaat 
ttgctgtgc cggcctaag gtcaatccg aggtcaatt 
ttgaagagt tcgcatgcg atgtttcaa gcgaaaaat 
gaaatggag aatgtcgag caggaggat gagagattt 
aataagcga tttgcgagg tgtaaggag gcgatgcaa 
gcgagaatt tttaataat tttattaat aaaacaaca 
ataccacaa gcgcccgtc cggcaatca cgtctgccc 
agaaaacag aaaattaaa cctttggaa aggatggat 
gaggagatt gtggtccag gaagtgatg gtgtcgtcg 
actggattc gtagacacg gcttctgga ggtgtcaat 
aaagagaac ttcgaagtg accgaatac gtcgaactg 
acggattcg gacgacgac tacgacccg aaaaaagtc 
atccgaaaa aagaagaaa caccgaagg aggaaaatc 
gagattacg ataagcgac gacagtgac gcttcgcac 
gacagcgtc atcgaattg gacgaaacc gacgacgaa 
gaaccgaaa agtcccgac gcccccaag cgacgacgt 
ggtcgtccc cccaaaaaa tcccgtgtg gaatcgaac 
gacatagct tcaaaaccc gaaattccc tgtccaaaa 
tgcgaaaaa accttccct agtcaaaac agcctcaag 
acacacctc caacaccat aatcttcaa agttcgatc 
aaaaacgcg ttacctgaa tcgaagtac aaatgcgac 
gattgcgcc atctctttc aaaaacagt atcctgttg 
aagaaccac aagtgtttg aaaaagttc acttgtccg 
gtttgtaaa aagaaattt cctgatatg tcgagtctg 
accggtcat aagcgagtc catgcgaaa gagcaactt 
gttaaaagt acagccgtt gtgcgggtt tcccccaaa 
aaaatgaaa ccagtgttg ccaaagtcg aaaagtccc 
cagaataaa tgtaaaacc tgcgggaaa atttgctcg 
tcggagcaa aacttgaac gttcacatg aaaacgcac 
aaggagtat gtttgtagc acttgttcg gcgactttc 
gtttcgcag tttatgttg gagaaacac gttagggaa 
aattgcgtc aagttcaag gtgcctcaa gttacgagg 
aggtcgatg aggatcaag aatgcctcg agtgcggag 
caactttcg ccgaaaagc cccaaaaga agccctatg 
aagggcagg gagaggtta acgatgagt tcactcaaa 
ctcaaatgt gacaaatgt aatgaccag tttgggact 
tttaaagca ctatttaga cacaaagtg gtgaagcac 
aacatggag acgccggat aaggaggcc ttggagaag 
gagcagcct gaggaaaag acacacgag ggggttcct 
gtagcgaag ggtttgcag aatgtgtgg acgggtgtc 
aacttaaaa ttggatttt taagattaa ggctatgta 
gatttgtaa ataggcttc ttatgtaca tttagcata 
ttgtgtatt ttaataaat ttttcaacc gtaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY