| General information | |||
| TC011184 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007425.3: 3136932..3142932 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC011184 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04981 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC011184 RA
MSLKKGAVS GPKRQFKEK IVQIYETLF KGETLPTSN TQFWNEFFL LKPKTGYID SEIQKMSQE QLFVAKSNV NLLVNSCIE TLNDSNNIR VVYAMQTLC AVIHAIFRK SSEFDSDSF LIGFENSEE KVQVLLSSC QGFLEGDVA HVLKCLSLK LLLILTTRT ENIDQNTLI EHVMNTSLF ECLLKLLCH SKTRQVHGY DVVLLLTLL VNYRKYDAA NPYVVKLSI LDDELALNG YGQVITSSL CEFCNQFNR ELIENQHTS WLTSLTTMV GNIFISEEG VLRTQQMRA NNAYLLAFY EAIHLNRNF ITTLAHTQI DGSSPPSPI NTLSPGSSP ADMTAINLS SQPSNLLVI FFQYCSIVM QDTKSEAGL NTVKLCFLI LCCITEDQY ANSLMHDVG LAFKVCLHK LPMRHRKVS PDQTTSSQP LVCSLLDLL VEFFLSHMM KKFPIELYV LCISVLQRI LCYQKRCRI RLNYVWKNL WAALLLVLR FLLQHENYL AKKMNIIDL SMKIVNIFN FFITYGDTF LPTPGSYDE LYYELIRMH QIFDNIYSM GLRYSTVEG EYKDDALKL NNSLINVRA IIKHFSPKI EQWLIAANL STPTEQQIL DVVKKNYDS LTLKLQDSL DQYDRYSEK PEHVTFFTH MVKSIVSDT SGSIGVTLA NLQKNELIS EIK
- >TC011184 RA
atgtcactg aaaaaaggg gccgtttct ggtcccaag cgccagttt aaggaaaag attgtgcaa atttatgaa accctcttc aagggagag actttgccg acgtctaac acgcaattc tggaacgaa ttttttcta ctgaagccc aaaactggc tacattgac agcgaaatc caaaaaatg tctcaagag caattattc gtagccaaa tcaaacgta aacctgcta gtgaacagc tgtattgag actctaaac gattcgaac aacatccga gtggtttac gcaatgcag actttgtgt gccgtaatt cacgcgatt tttcgaaaa tcgtctgaa ttcgactcg gactcgttt ttgataggt ttcgaaaac agtgaagag aaggtccaa gtgttgctg tcatcgtgt cagggtttt cttgaaggt gatgtggct catgtcttg aaatgtcta tccttaaaa ctgctcttg attctcaca actcggact gaaaatatc gaccaaaac actttaatt gagcacgtg atgaataca tcacttttc gagtgtctt ttaaaactg ctttgccac agtaaaacg cgtcaagtg cacggttac gacgttgta ctgttgctc actttgctc gtaaattac cggaaatac gatgccgcc aatccgtac gttgttaaa ttgtcgatt ttagacgac gaattggcg ctaaacggt tatggacaa gtgatcacc tcgtcgttg tgcgaattt tgtaatcag tttaataga gaattaatc gagaatcag cacacttcg tggttgact tcgctcacg accatggtg ggcaatatt ttcatatct gaagaggga gttttaaga acgcaacaa atgcgagcg aataacgcg tatttgttg gcgttttac gaagccatc catttgaat aggaatttt ataaccacg ctagcacac acccagatt gacggcagt tccccaccc tcccctatc aatacgctg agtcctgga agtagtcct gcggacatg acggctatt aacttgagt tcgcagcct tccaatctc ttagtcata tttttccag tattgttcg atcgtgatg caggacact aagagcgaa gccggttta aacacagta aaactctgt ttcctcatc ctttgctgc atcaccgaa gaccaatac gccaactcg ttgatgcac gacgtgggt ctagccttc aaagtgtgc ctccacaag ctccccatg cgccataga aaagtctca ccggaccaa accacctca tcacaaccc ctagtctgc tccttgcta gacttgcta gttgagttt ttcctcagc catatgatg aaaaaattc cctattgaa ctctacgtt ttgtgcata agcgttctc caacgcatc ctttgctac caaaaacgc tgcagaatt cgcctgaat tacgtgtgg aaaaaccta tgggcggcg ttacttctc gttttacgg tttttgctc caacacgag aactatctt gccaagaaa atgaacatc atcgacttg tcaatgaaa atagtcaat atttttaat ttttttata acttatggg gacacgttt ttgccaacg cctggcagc tatgacgaa ttgtattac gagttgatt cggatgcac cagattttt gataatatc tactcaatg ggtctgcgc tactcgact gtggaggga gaatacaaa gacgacgct ttgaaactg aacaatagt ctgatcaac gtgcgagca attattaag cacttttcg ccaaaaatc gagcagtgg ctgatcgct gccaactta tcaacacca accgaacaa caaatccta gacgttgtc aagaagaat tatgatagt ctgactttg aaattgcaa gactcctta gaccagtat gacagatat agtgagaaa ccagaacac gtgaccttc ttcacacat atggtgaag agtatagtg tctgacacc agtggcagt attggggtg acgttagcc aacttgcaa aaaaatgag cttataagt gaaataaaa taa
- >TC011184 RA
aacgtcagt gtttgttat catgtgaat tatttattt gcgccgaaa agcggttta tttttgggt ataattttc caccttatt gttaaaaac tgatacttg aaaaatgtc actgaaaaa aggggccgt ttctggtcc caagcgcca gtttaagga aaagattgt gcaaattta tgaaaccct cttcaaggg agagacttt gccgacgtc taacacgca attctggaa cgaattttt tctactgaa gcccaaaac tggctacat tgacagcga aatccaaaa aatgtctca agagcaatt attcgtagc caaatcaaa cgtaaacct gctagtgaa cagctgtat tgagactct aaacgattc gaacaacat ccgagtggt ttacgcaat gcagacttt gtgtgccgt aattcacgc gatttttcg aaaatcgtc tgaattcga ctcggactc gtttttgat aggtttcga aaacagtga agagaaggt ccaagtgtt gctgtcatc gtgtcaggg ttttcttga aggtgatgt ggctcatgt cttgaaatg tctatcctt aaaactgct cttgattct cacaactcg gactgaaaa tatcgacca aaacacttt aattgagca cgtgatgaa tacatcact tttcgagtg tcttttaaa actgctttg ccacagtaa aacgcgtca agtgcacgg ttacgacgt tgtactgtt gctcacttt gctcgtaaa ttaccggaa atacgatgc cgccaatcc gtacgttgt taaattgtc gattttaga cgacgaatt ggcgctaaa cggttatgg acaagtgat cacctcgtc gttgtgcga attttgtaa tcagtttaa tagagaatt aatcgagaa tcagcacac ttcgtggtt gacttcgct cacgaccat ggtgggcaa tattttcat atctgaaga gggagtttt aagaacgca acaaatgcg agcgaataa cgcgtattt gttggcgtt ttacgaagc catccattt gaataggaa ttttataac cacgctagc acacaccca gattgacgg cagttcccc accctcccc tatcaatac gctgagtcc tggaagtag tcctgcgga catgacggc tattaactt gagttcgca gccttccaa tctcttagt catattttt ccagtattg ttcgatcgt gatgcagga cactaagag cgaagccgg tttaaacac agtaaaact ctgtttcct catcctttg ctgcatcac cgaagacca atacgccaa ctcgttgat gcacgacgt gggtctagc cttcaaagt gtgcctcca caagctccc catgcgcca tagaaaagt ctcaccgga ccaaaccac ctcatcaca acccctagt ctgctcctt gctagactt gctagttga gtttttcct cagccatat gatgaaaaa attccctat tgaactcta cgttttgtg cataagcgt tctccaacg catcctttg ctaccaaaa acgctgcag aattcgcct gaattacgt gtggaaaaa cctatgggc ggcgttact tctcgtttt acggttttt gctccaaca cgagaacta tcttgccaa gaaaatgaa catcatcga cttgtcaat gaaaatagt caatatttt taatttttt tataactta tggggacac gtttttgcc aacgcctgg cagctatga cgaattgta ttacgagtt gattcggat gcaccagat ttttgataa tatctactc aatgggtct gcgctactc gactgtgga gggagaata caaagacga cgctttgaa actgaacaa tagtctgat caacgtgcg agcaattat taagcactt ttcgccaaa aatcgagca gtggctgat cgctgccaa cttatcaac accaaccga acaacaaat cctagacgt tgtcaagaa gaattatga tagtctgac tttgaaatt gcaagactc cttagacca gtatgacag atatagtga gaaaccaga acacgtgac cttcttcac acatatggt gaagagtat agtgtctga caccagtgg cagtattgg ggtgacgtt agccaactt gcaaaaaaa tgagcttat aagtgaaat aaaataaag attaatttt atgacttta ttgagagtt ttcaatgcg gttttgatt ccaaacgtc ctcgtagaa ccgcgggtc gcttattcg caaacttgt gcgcttgta gaagtagta gtagatcgg agtcactga aaaaattac gtgttctta cacaaaata cataaagaa acattaa
