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Gene information
General information
TC011184 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 3136932..3142932
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011184
    iBeetle screen iB_04981

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011184 RA
      MSLKKGAVS GPKRQFKEK IVQIYETLF KGETLPTSN 
TQFWNEFFL LKPKTGYID SEIQKMSQE QLFVAKSNV 
NLLVNSCIE TLNDSNNIR VVYAMQTLC AVIHAIFRK 
SSEFDSDSF LIGFENSEE KVQVLLSSC QGFLEGDVA 
HVLKCLSLK LLLILTTRT ENIDQNTLI EHVMNTSLF 
ECLLKLLCH SKTRQVHGY DVVLLLTLL VNYRKYDAA 
NPYVVKLSI LDDELALNG YGQVITSSL CEFCNQFNR 
ELIENQHTS WLTSLTTMV GNIFISEEG VLRTQQMRA 
NNAYLLAFY EAIHLNRNF ITTLAHTQI DGSSPPSPI 
NTLSPGSSP ADMTAINLS SQPSNLLVI FFQYCSIVM 
QDTKSEAGL NTVKLCFLI LCCITEDQY ANSLMHDVG 
LAFKVCLHK LPMRHRKVS PDQTTSSQP LVCSLLDLL 
VEFFLSHMM KKFPIELYV LCISVLQRI LCYQKRCRI 
RLNYVWKNL WAALLLVLR FLLQHENYL AKKMNIIDL 
SMKIVNIFN FFITYGDTF LPTPGSYDE LYYELIRMH 
QIFDNIYSM GLRYSTVEG EYKDDALKL NNSLINVRA 
IIKHFSPKI EQWLIAANL STPTEQQIL DVVKKNYDS 
LTLKLQDSL DQYDRYSEK PEHVTFFTH MVKSIVSDT 
SGSIGVTLA NLQKNELIS EIK
    CDS sequence
    • >TC011184 RA
      atgtcactg aaaaaaggg gccgtttct ggtcccaag 
cgccagttt aaggaaaag attgtgcaa atttatgaa 
accctcttc aagggagag actttgccg acgtctaac 
acgcaattc tggaacgaa ttttttcta ctgaagccc 
aaaactggc tacattgac agcgaaatc caaaaaatg 
tctcaagag caattattc gtagccaaa tcaaacgta 
aacctgcta gtgaacagc tgtattgag actctaaac 
gattcgaac aacatccga gtggtttac gcaatgcag 
actttgtgt gccgtaatt cacgcgatt tttcgaaaa 
tcgtctgaa ttcgactcg gactcgttt ttgataggt 
ttcgaaaac agtgaagag aaggtccaa gtgttgctg 
tcatcgtgt cagggtttt cttgaaggt gatgtggct 
catgtcttg aaatgtcta tccttaaaa ctgctcttg 
attctcaca actcggact gaaaatatc gaccaaaac 
actttaatt gagcacgtg atgaataca tcacttttc 
gagtgtctt ttaaaactg ctttgccac agtaaaacg 
cgtcaagtg cacggttac gacgttgta ctgttgctc 
actttgctc gtaaattac cggaaatac gatgccgcc 
aatccgtac gttgttaaa ttgtcgatt ttagacgac 
gaattggcg ctaaacggt tatggacaa gtgatcacc 
tcgtcgttg tgcgaattt tgtaatcag tttaataga 
gaattaatc gagaatcag cacacttcg tggttgact 
tcgctcacg accatggtg ggcaatatt ttcatatct 
gaagaggga gttttaaga acgcaacaa atgcgagcg 
aataacgcg tatttgttg gcgttttac gaagccatc 
catttgaat aggaatttt ataaccacg ctagcacac 
acccagatt gacggcagt tccccaccc tcccctatc 
aatacgctg agtcctgga agtagtcct gcggacatg 
acggctatt aacttgagt tcgcagcct tccaatctc 
ttagtcata tttttccag tattgttcg atcgtgatg 
caggacact aagagcgaa gccggttta aacacagta 
aaactctgt ttcctcatc ctttgctgc atcaccgaa 
gaccaatac gccaactcg ttgatgcac gacgtgggt 
ctagccttc aaagtgtgc ctccacaag ctccccatg 
cgccataga aaagtctca ccggaccaa accacctca 
tcacaaccc ctagtctgc tccttgcta gacttgcta 
gttgagttt ttcctcagc catatgatg aaaaaattc 
cctattgaa ctctacgtt ttgtgcata agcgttctc 
caacgcatc ctttgctac caaaaacgc tgcagaatt 
cgcctgaat tacgtgtgg aaaaaccta tgggcggcg 
ttacttctc gttttacgg tttttgctc caacacgag 
aactatctt gccaagaaa atgaacatc atcgacttg 
tcaatgaaa atagtcaat atttttaat ttttttata 
acttatggg gacacgttt ttgccaacg cctggcagc 
tatgacgaa ttgtattac gagttgatt cggatgcac 
cagattttt gataatatc tactcaatg ggtctgcgc 
tactcgact gtggaggga gaatacaaa gacgacgct 
ttgaaactg aacaatagt ctgatcaac gtgcgagca 
attattaag cacttttcg ccaaaaatc gagcagtgg 
ctgatcgct gccaactta tcaacacca accgaacaa 
caaatccta gacgttgtc aagaagaat tatgatagt 
ctgactttg aaattgcaa gactcctta gaccagtat 
gacagatat agtgagaaa ccagaacac gtgaccttc 
ttcacacat atggtgaag agtatagtg tctgacacc 
agtggcagt attggggtg acgttagcc aacttgcaa 
aaaaatgag cttataagt gaaataaaa taa
    mRNA sequence
    • >TC011184 RA
      aacgtcagt gtttgttat catgtgaat tatttattt 
gcgccgaaa agcggttta tttttgggt ataattttc 
caccttatt gttaaaaac tgatacttg aaaaatgtc 
actgaaaaa aggggccgt ttctggtcc caagcgcca 
gtttaagga aaagattgt gcaaattta tgaaaccct 
cttcaaggg agagacttt gccgacgtc taacacgca 
attctggaa cgaattttt tctactgaa gcccaaaac 
tggctacat tgacagcga aatccaaaa aatgtctca 
agagcaatt attcgtagc caaatcaaa cgtaaacct 
gctagtgaa cagctgtat tgagactct aaacgattc 
gaacaacat ccgagtggt ttacgcaat gcagacttt 
gtgtgccgt aattcacgc gatttttcg aaaatcgtc 
tgaattcga ctcggactc gtttttgat aggtttcga 
aaacagtga agagaaggt ccaagtgtt gctgtcatc 
gtgtcaggg ttttcttga aggtgatgt ggctcatgt 
cttgaaatg tctatcctt aaaactgct cttgattct 
cacaactcg gactgaaaa tatcgacca aaacacttt 
aattgagca cgtgatgaa tacatcact tttcgagtg 
tcttttaaa actgctttg ccacagtaa aacgcgtca 
agtgcacgg ttacgacgt tgtactgtt gctcacttt 
gctcgtaaa ttaccggaa atacgatgc cgccaatcc 
gtacgttgt taaattgtc gattttaga cgacgaatt 
ggcgctaaa cggttatgg acaagtgat cacctcgtc 
gttgtgcga attttgtaa tcagtttaa tagagaatt 
aatcgagaa tcagcacac ttcgtggtt gacttcgct 
cacgaccat ggtgggcaa tattttcat atctgaaga 
gggagtttt aagaacgca acaaatgcg agcgaataa 
cgcgtattt gttggcgtt ttacgaagc catccattt 
gaataggaa ttttataac cacgctagc acacaccca 
gattgacgg cagttcccc accctcccc tatcaatac 
gctgagtcc tggaagtag tcctgcgga catgacggc 
tattaactt gagttcgca gccttccaa tctcttagt 
catattttt ccagtattg ttcgatcgt gatgcagga 
cactaagag cgaagccgg tttaaacac agtaaaact 
ctgtttcct catcctttg ctgcatcac cgaagacca 
atacgccaa ctcgttgat gcacgacgt gggtctagc 
cttcaaagt gtgcctcca caagctccc catgcgcca 
tagaaaagt ctcaccgga ccaaaccac ctcatcaca 
acccctagt ctgctcctt gctagactt gctagttga 
gtttttcct cagccatat gatgaaaaa attccctat 
tgaactcta cgttttgtg cataagcgt tctccaacg 
catcctttg ctaccaaaa acgctgcag aattcgcct 
gaattacgt gtggaaaaa cctatgggc ggcgttact 
tctcgtttt acggttttt gctccaaca cgagaacta 
tcttgccaa gaaaatgaa catcatcga cttgtcaat 
gaaaatagt caatatttt taatttttt tataactta 
tggggacac gtttttgcc aacgcctgg cagctatga 
cgaattgta ttacgagtt gattcggat gcaccagat 
ttttgataa tatctactc aatgggtct gcgctactc 
gactgtgga gggagaata caaagacga cgctttgaa 
actgaacaa tagtctgat caacgtgcg agcaattat 
taagcactt ttcgccaaa aatcgagca gtggctgat 
cgctgccaa cttatcaac accaaccga acaacaaat 
cctagacgt tgtcaagaa gaattatga tagtctgac 
tttgaaatt gcaagactc cttagacca gtatgacag 
atatagtga gaaaccaga acacgtgac cttcttcac 
acatatggt gaagagtat agtgtctga caccagtgg 
cagtattgg ggtgacgtt agccaactt gcaaaaaaa 
tgagcttat aagtgaaat aaaataaag attaatttt 
atgacttta ttgagagtt ttcaatgcg gttttgatt 
ccaaacgtc ctcgtagaa ccgcgggtc gcttattcg 
caaacttgt gcgcttgta gaagtagta gtagatcgg 
agtcactga aaaaattac gtgttctta cacaaaata 
cataaagaa acattaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY