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Gene information
General information
TC011184 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 3136932..3142932
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011184
    iBeetle screen iB_04981

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011184 RA
      MSLKKGAVS GPKRQFKEK IVQIYETLF KGETLPTSN 
      TQFWNEFFL LKPKTGYID SEIQKMSQE QLFVAKSNV 
      NLLVNSCIE TLNDSNNIR VVYAMQTLC AVIHAIFRK 
      SSEFDSDSF LIGFENSEE KVQVLLSSC QGFLEGDVA 
      HVLKCLSLK LLLILTTRT ENIDQNTLI EHVMNTSLF 
      ECLLKLLCH SKTRQVHGY DVVLLLTLL VNYRKYDAA 
      NPYVVKLSI LDDELALNG YGQVITSSL CEFCNQFNR 
      ELIENQHTS WLTSLTTMV GNIFISEEG VLRTQQMRA 
      NNAYLLAFY EAIHLNRNF ITTLAHTQI DGSSPPSPI 
      NTLSPGSSP ADMTAINLS SQPSNLLVI FFQYCSIVM 
      QDTKSEAGL NTVKLCFLI LCCITEDQY ANSLMHDVG 
      LAFKVCLHK LPMRHRKVS PDQTTSSQP LVCSLLDLL 
      VEFFLSHMM KKFPIELYV LCISVLQRI LCYQKRCRI 
      RLNYVWKNL WAALLLVLR FLLQHENYL AKKMNIIDL 
      SMKIVNIFN FFITYGDTF LPTPGSYDE LYYELIRMH 
      QIFDNIYSM GLRYSTVEG EYKDDALKL NNSLINVRA 
      IIKHFSPKI EQWLIAANL STPTEQQIL DVVKKNYDS 
      LTLKLQDSL DQYDRYSEK PEHVTFFTH MVKSIVSDT 
      SGSIGVTLA NLQKNELIS EIK
    CDS sequence
    • >TC011184 RA
      atgtcactg aaaaaaggg gccgtttct ggtcccaag 
      cgccagttt aaggaaaag attgtgcaa atttatgaa 
      accctcttc aagggagag actttgccg acgtctaac 
      acgcaattc tggaacgaa ttttttcta ctgaagccc 
      aaaactggc tacattgac agcgaaatc caaaaaatg 
      tctcaagag caattattc gtagccaaa tcaaacgta 
      aacctgcta gtgaacagc tgtattgag actctaaac 
      gattcgaac aacatccga gtggtttac gcaatgcag 
      actttgtgt gccgtaatt cacgcgatt tttcgaaaa 
      tcgtctgaa ttcgactcg gactcgttt ttgataggt 
      ttcgaaaac agtgaagag aaggtccaa gtgttgctg 
      tcatcgtgt cagggtttt cttgaaggt gatgtggct 
      catgtcttg aaatgtcta tccttaaaa ctgctcttg 
      attctcaca actcggact gaaaatatc gaccaaaac 
      actttaatt gagcacgtg atgaataca tcacttttc 
      gagtgtctt ttaaaactg ctttgccac agtaaaacg 
      cgtcaagtg cacggttac gacgttgta ctgttgctc 
      actttgctc gtaaattac cggaaatac gatgccgcc 
      aatccgtac gttgttaaa ttgtcgatt ttagacgac 
      gaattggcg ctaaacggt tatggacaa gtgatcacc 
      tcgtcgttg tgcgaattt tgtaatcag tttaataga 
      gaattaatc gagaatcag cacacttcg tggttgact 
      tcgctcacg accatggtg ggcaatatt ttcatatct 
      gaagaggga gttttaaga acgcaacaa atgcgagcg 
      aataacgcg tatttgttg gcgttttac gaagccatc 
      catttgaat aggaatttt ataaccacg ctagcacac 
      acccagatt gacggcagt tccccaccc tcccctatc 
      aatacgctg agtcctgga agtagtcct gcggacatg 
      acggctatt aacttgagt tcgcagcct tccaatctc 
      ttagtcata tttttccag tattgttcg atcgtgatg 
      caggacact aagagcgaa gccggttta aacacagta 
      aaactctgt ttcctcatc ctttgctgc atcaccgaa 
      gaccaatac gccaactcg ttgatgcac gacgtgggt 
      ctagccttc aaagtgtgc ctccacaag ctccccatg 
      cgccataga aaagtctca ccggaccaa accacctca 
      tcacaaccc ctagtctgc tccttgcta gacttgcta 
      gttgagttt ttcctcagc catatgatg aaaaaattc 
      cctattgaa ctctacgtt ttgtgcata agcgttctc 
      caacgcatc ctttgctac caaaaacgc tgcagaatt 
      cgcctgaat tacgtgtgg aaaaaccta tgggcggcg 
      ttacttctc gttttacgg tttttgctc caacacgag 
      aactatctt gccaagaaa atgaacatc atcgacttg 
      tcaatgaaa atagtcaat atttttaat ttttttata 
      acttatggg gacacgttt ttgccaacg cctggcagc 
      tatgacgaa ttgtattac gagttgatt cggatgcac 
      cagattttt gataatatc tactcaatg ggtctgcgc 
      tactcgact gtggaggga gaatacaaa gacgacgct 
      ttgaaactg aacaatagt ctgatcaac gtgcgagca 
      attattaag cacttttcg ccaaaaatc gagcagtgg 
      ctgatcgct gccaactta tcaacacca accgaacaa 
      caaatccta gacgttgtc aagaagaat tatgatagt 
      ctgactttg aaattgcaa gactcctta gaccagtat 
      gacagatat agtgagaaa ccagaacac gtgaccttc 
      ttcacacat atggtgaag agtatagtg tctgacacc 
      agtggcagt attggggtg acgttagcc aacttgcaa 
      aaaaatgag cttataagt gaaataaaa taa
    mRNA sequence
    • >TC011184 RA
      aacgtcagt gtttgttat catgtgaat tatttattt 
      gcgccgaaa agcggttta tttttgggt ataattttc 
      caccttatt gttaaaaac tgatacttg aaaaatgtc 
      actgaaaaa aggggccgt ttctggtcc caagcgcca 
      gtttaagga aaagattgt gcaaattta tgaaaccct 
      cttcaaggg agagacttt gccgacgtc taacacgca 
      attctggaa cgaattttt tctactgaa gcccaaaac 
      tggctacat tgacagcga aatccaaaa aatgtctca 
      agagcaatt attcgtagc caaatcaaa cgtaaacct 
      gctagtgaa cagctgtat tgagactct aaacgattc 
      gaacaacat ccgagtggt ttacgcaat gcagacttt 
      gtgtgccgt aattcacgc gatttttcg aaaatcgtc 
      tgaattcga ctcggactc gtttttgat aggtttcga 
      aaacagtga agagaaggt ccaagtgtt gctgtcatc 
      gtgtcaggg ttttcttga aggtgatgt ggctcatgt 
      cttgaaatg tctatcctt aaaactgct cttgattct 
      cacaactcg gactgaaaa tatcgacca aaacacttt 
      aattgagca cgtgatgaa tacatcact tttcgagtg 
      tcttttaaa actgctttg ccacagtaa aacgcgtca 
      agtgcacgg ttacgacgt tgtactgtt gctcacttt 
      gctcgtaaa ttaccggaa atacgatgc cgccaatcc 
      gtacgttgt taaattgtc gattttaga cgacgaatt 
      ggcgctaaa cggttatgg acaagtgat cacctcgtc 
      gttgtgcga attttgtaa tcagtttaa tagagaatt 
      aatcgagaa tcagcacac ttcgtggtt gacttcgct 
      cacgaccat ggtgggcaa tattttcat atctgaaga 
      gggagtttt aagaacgca acaaatgcg agcgaataa 
      cgcgtattt gttggcgtt ttacgaagc catccattt 
      gaataggaa ttttataac cacgctagc acacaccca 
      gattgacgg cagttcccc accctcccc tatcaatac 
      gctgagtcc tggaagtag tcctgcgga catgacggc 
      tattaactt gagttcgca gccttccaa tctcttagt 
      catattttt ccagtattg ttcgatcgt gatgcagga 
      cactaagag cgaagccgg tttaaacac agtaaaact 
      ctgtttcct catcctttg ctgcatcac cgaagacca 
      atacgccaa ctcgttgat gcacgacgt gggtctagc 
      cttcaaagt gtgcctcca caagctccc catgcgcca 
      tagaaaagt ctcaccgga ccaaaccac ctcatcaca 
      acccctagt ctgctcctt gctagactt gctagttga 
      gtttttcct cagccatat gatgaaaaa attccctat 
      tgaactcta cgttttgtg cataagcgt tctccaacg 
      catcctttg ctaccaaaa acgctgcag aattcgcct 
      gaattacgt gtggaaaaa cctatgggc ggcgttact 
      tctcgtttt acggttttt gctccaaca cgagaacta 
      tcttgccaa gaaaatgaa catcatcga cttgtcaat 
      gaaaatagt caatatttt taatttttt tataactta 
      tggggacac gtttttgcc aacgcctgg cagctatga 
      cgaattgta ttacgagtt gattcggat gcaccagat 
      ttttgataa tatctactc aatgggtct gcgctactc 
      gactgtgga gggagaata caaagacga cgctttgaa 
      actgaacaa tagtctgat caacgtgcg agcaattat 
      taagcactt ttcgccaaa aatcgagca gtggctgat 
      cgctgccaa cttatcaac accaaccga acaacaaat 
      cctagacgt tgtcaagaa gaattatga tagtctgac 
      tttgaaatt gcaagactc cttagacca gtatgacag 
      atatagtga gaaaccaga acacgtgac cttcttcac 
      acatatggt gaagagtat agtgtctga caccagtgg 
      cagtattgg ggtgacgtt agccaactt gcaaaaaaa 
      tgagcttat aagtgaaat aaaataaag attaatttt 
      atgacttta ttgagagtt ttcaatgcg gttttgatt 
      ccaaacgtc ctcgtagaa ccgcgggtc gcttattcg 
      caaacttgt gcgcttgta gaagtagta gtagatcgg 
      agtcactga aaaaattac gtgttctta cacaaaata 
      cataaagaa acattaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY