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Gene information
General information
TC011180 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 3167632..3172362
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011180
    iBeetle screen iB_04980

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    remarks: animals lost,
    Pupal morphology
    remarks: animals lost,
    Adult morphology
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011180 RA
      MQSTTILVA LSAFTAFIY FLAEYFSKK QRLLRFHAA 
      KLQGPPALP LIGSAYYLF GSNKSIVNN VIHLVKKYK 
      SPWKIWLGR QLYIVVTEP EDIEVVLNK ALEKAENYN 
      FLACLLQEG LFTGPVEKW KRHRKVILS TFSMPILKS 
      FVEIFSENS LRMLPKLDK FVGKGTFDV CPILTNTAL 
      EITCETAMG TKIYEQAHS DYAYNLSKA LELVFMRMF 
      QYWLHPNFI WSLSIYSKQ LKQLSKQLE IFVQGIVTE 
      KKVQYRDKK HDLCLETKR RKCFIDHLI EMSEDDNWH 
      DHELLEEAQ TMVAAGSES LGSVKSFTL IMLGMHPLI 
      QDKVYNEMY NIFGPSDRT VTPDDLTEM TYLDMVIKE 
      TLRLFPVTA AVGRRVSQD IVTDRYTLP EGCECIVSI 
      LSAHRNPKI WPKPLDFNP DRFLPEEVA KRHPYSYLP 
      FSNGPRNCI GFKYAMMAI KTVISTIVR RYKISTEFK 
      SVPEIEFSP GVVLKSRKG YRTQLESRC
    CDS sequence
    • >TC011180 RA
      atgcaatca acaacgatt ttagtggct ttgagtgct 
      ttcacagcc tttatctat ttcctggcc gagtatttt 
      tcgaaaaaa cagagactt ttgcggttc catgccgcc 
      aaactccaa ggcccccct gctttgccc ctaatcgga 
      agtgcgtat tatctgttc gggagcaac aaaagtata 
      gtgaacaat gtcatccac ttggtaaaa aaatacaaa 
      tcgccctgg aaaatttgg ctggggcgc caattgtac 
      attgttgtg actgagccc gaagatatt gaagttgtg 
      ttaaataaa gcgttagaa aaagcggaa aattacaac 
      tttctggcg tgtttacta caagagggg ctttttaca 
      ggaccggtc gagaagtgg aaaaggcac cggaaggtc 
      attttgagc actttcagt atgccgatt ttgaaaagt 
      tttgtggaa attttttcg gaaaattcg cttagaatg 
      ttgccaaag ttggataag tttgtcggg aaaggaacg 
      tttgatgtt tgcccgatt ttgaccaat acggccctg 
      gaaattaca tgtgaaaca gcaatgggg acaaaaatc 
      tacgaacag gcgcattca gattacgct tacaacttg 
      tccaaggct ttggaactg gtttttatg cgaatgttc 
      caatattgg ttgcatcct aatttcatc tggagtctg 
      agcatctat tcaaaacag ttgaaacaa ctatcaaaa 
      caactggaa attttcgtc caaggcatt gtcacagag 
      aaaaaagtt caatatcgg gacaaaaaa cacgattta 
      tgtttagag acaaaaaga agaaaatgt ttcattgat 
      catttgatc gaaatgtcg gaggatgat aattggcat 
      gatcacgag cttctggag gaagcccaa actatggtg 
      gctgcgggc agcgagtcc ttggggtcc gtgaaaagt 
      tttactttg attatgcta ggaatgcac cccttgatt 
      caggacaaa gtctacaac gaaatgtac aacattttc 
      ggccccagt gaccgaact gtcaccccc gacgacttg 
      accgaaatg acatacctg gacatggtc atcaaggaa 
      accttgcga ctgtttcca gtgactgca gcagtaggg 
      cgacgtgtc agccaggat atagtgact gacagatac 
      acactaccc gaaggttgc gaatgcata gttagcatt 
      ttatccgcc cacagaaac ccgaaaatt tggccaaaa 
      ccgcttgat ttcaaccct gataggttt ctcccagaa 
      gaggtggcc aaacgccac ccttattcc tacttgcct 
      ttcagtaac ggacctcgg aactgtatt ggtttcaaa 
      tacgcaatg atggccata aaaactgta atttcgacg 
      attgtccgc cgctacaaa atctcaacc gagttcaaa 
      tccgtccct gaaatcgaa ttctcacct ggcgttgtc 
      ttgaaatcc agaaagggc tacaggaca caactggag 
      tccaggtgt taa
    mRNA sequence
    • >TC011180 RA
      tcgaacaaa atgcaatca acaacgatt ttagtggct 
      ttgagtgct ttcacagcc tttatctat ttcctggcc 
      gagtatttt tcgaaaaaa cagagactt ttgcggttc 
      catgccgcc aaactccaa ggcccccct gctttgccc 
      ctaatcgga agtgcgtat tatctgttc gggagcaac 
      aaaagtata gtgaacaat gtcatccac ttggtaaaa 
      aaatacaaa tcgccctgg aaaatttgg ctggggcgc 
      caattgtac attgttgtg actgagccc gaagatatt 
      gaagttgtg ttaaataaa gcgttagaa aaagcggaa 
      aattacaac tttctggcg tgtttacta caagagggg 
      ctttttaca ggaccggtc gagaagtgg aaaaggcac 
      cggaaggtc attttgagc actttcagt atgccgatt 
      ttgaaaagt tttgtggaa attttttcg gaaaattcg 
      cttagaatg ttgccaaag ttggataag tttgtcggg 
      aaaggaacg tttgatgtt tgcccgatt ttgaccaat 
      acggccctg gaaattaca tgtgaaaca gcaatgggg 
      acaaaaatc tacgaacag gcgcattca gattacgct 
      tacaacttg tccaaggct ttggaactg gtttttatg 
      cgaatgttc caatattgg ttgcatcct aatttcatc 
      tggagtctg agcatctat tcaaaacag ttgaaacaa 
      ctatcaaaa caactggaa attttcgtc caaggcatt 
      gtcacagag aaaaaagtt caatatcgg gacaaaaaa 
      cacgattta tgtttagag acaaaaaga agaaaatgt 
      ttcattgat catttgatc gaaatgtcg gaggatgat 
      aattggcat gatcacgag cttctggag gaagcccaa 
      actatggtg gctgcgggc agcgagtcc ttggggtcc 
      gtgaaaagt tttactttg attatgcta ggaatgcac 
      cccttgatt caggacaaa gtctacaac gaaatgtac 
      aacattttc ggccccagt gaccgaact gtcaccccc 
      gacgacttg accgaaatg acatacctg gacatggtc 
      atcaaggaa accttgcga ctgtttcca gtgactgca 
      gcagtaggg cgacgtgtc agccaggat atagtgact 
      gacagatac acactaccc gaaggttgc gaatgcata 
      gttagcatt ttatccgcc cacagaaac ccgaaaatt 
      tggccaaaa ccgcttgat ttcaaccct gataggttt 
      ctcccagaa gaggtggcc aaacgccac ccttattcc 
      tacttgcct ttcagtaac ggacctcgg aactgtatt 
      ggtttcaaa tacgcaatg atggccata aaaactgta 
      atttcgacg attgtccgc cgctacaaa atctcaacc 
      gagttcaaa tccgtccct gaaatcgaa ttctcacct 
      ggcgttgtc ttgaaatcc agaaagggc tacaggaca 
      caactggag tccaggtgt taatagttt aatgtacat 
      atatcataa ataaaatta atacaaatc c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY