Status
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Gene information
General information
TC011170 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 3350858..3372872
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011170
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04979

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011170 RA
      MRKKLLVWI ITLSTCLRT TLGLYKIGV GIADCTGPA 
      AEVTFMGYG KSNQKGCGI HLRQFSRAF VIDDGSQRA 
      AFVSVDAGM MAYGVRKAV ISRLGKQYK DVYTESNLI 
      LSGTHTHSA PGGFLMDFM YDIPSLGFV KETFDALVD 
      GITRSVVRA HENMVDGKI FITTGELIG ANINRSPMS 
      YLLNPKEER EKYKYNVDK EMVQLKLVN SANSLIGVI 
      SWFPVHPTS MNNTNCLIT SDNVGYASV LLEKSLNKN 
      SLVGQGRIV AAFASTNLG DVSPNTKGP RCVTTGEEC 
      DVVSSTCDG QAKFCVAAG PGEDMFEST KIIAEKLFE 
      KSKILLQSS EAKEVTGPV RHIHQYVDM PSQNVTITL 
      PNGTITHVT GCLPAMGYS FAAGTTDGV GEFGFAQGT 
      RSTNSHWDT LRDLLFKPT EEDIRCQHP KPILINAGR 
      ISIPYQWQP RIVSTQIVQ VGEVLLIAV PGEFTTMSG 
      RRLRNGVQE TAVRSGASP GTKAIITGL SNVYTSYIT 
      TPEEYQMQR YEGASTIYG PHTLTIYIE KYKQLTKAL 
      LKGEKLDSG PLPADLSQR VISLQPRVM FDTPGLMHH 
      FGDCLLQPP KSARVGSKV RAKFVSGHP RNNLMTEKT 
      FLTVEKLAD NGKWIVLAT DADWETKFT WTRTSTLIG 
      GSEATIEWE IPSDATPGL YRIHHFGHF QHIFGGVHP 
      YSGATDTFQ VTK
    CDS sequence
    • >TC011170 RA
      atgcgaaaa aagctctta gtctggatt ataacatta 
      tcgacatgt ctccgcacg actttgggg ctctacaaa 
      attggggtc ggaatagcc gactgtacg ggaccggca 
      gctgaagtc acatttatg ggttatgga aaatcgaat 
      cagaagggt tgcgggata catttgcgt caattttct 
      cgcgccttt gtcatcgat gatgggtct cagagggct 
      gctttcgtt agcgtggac gctggaatg atggcctat 
      ggggtccgc aaggctgtc atttctcgc ctcggcaag 
      caatacaaa gacgtctac actgagagt aacctaatt 
      ttgagcggg acacacact cacagcgct cccggagga 
      tttctgatg gattttatg tatgatatt ccaagtttg 
      ggatttgtg aaagaaacg tttgacgct cttgttgat 
      ggaatcacg aggtctgtt gtcagggcg catgagaat 
      atggtagat gggaagata tttatcacg acaggggag 
      ttgattgga gcgaatatt aatagaagt ccaatgtcc 
      tatttgctg aatcctaag gaggaacgt gaaaaatac 
      aaatacaat gtggacaaa gaaatggtg cagctgaag 
      ctcgtcaac tccgccaac tcgctcatc ggcgtcatc 
      agctggttt cccgtccat cccacgtcc atgaacaac 
      acaaactgc ctaatcacc tccgataat gtgggctac 
      gcgtccgtc ctgctcgaa aaatctctg aataaaaat 
      tcgctcgtt ggccaagga cggattgtg gcggctttc 
      gcgtccacc aatttaggg gacgtttca ccgaacacc 
      aaagggccg aggtgtgtg acgacgggg gaggaatgc 
      gatgttgtg tcgagtacg tgcgacgga caagcaaag 
      ttttgtgtg gcggcagga ccgggcgag gatatgttt 
      gagagtacg aaaataatc gcagagaaa ctttttgaa 
      aaatcgaag attctttta caatcctct gaggcgaaa 
      gaggtgacc ggtcctgtc agacacatt caccaatac 
      gtggacatg ccgtctcaa aacgtgacc atcacacta 
      cccaacggt accataacc catgtcact gggtgcctc 
      ccagcgatg ggttacagc ttcgctgct ggtaccacc 
      gacggagtg ggcgagttt ggctttgcc caaggcacc 
      agatcgaca aactcccat tgggacact ctgcgagac 
      ttgctgttc aagccgacg gaagaagac atcaggtgc 
      cagcatcct aaaccaatt ttaatcaac gccggaagg 
      atttccatc ccttatcag tggcaaccg cggattgtt 
      tccacgcaa atcgtccaa gtgggtgaa gtgcttcta 
      attgccgtt cccggcgaa ttcaccaca atgtctggc 
      agacgtctc cgaaatggc gtgcaggaa accgccgtc 
      agaagcggg gcttcccct ggcacaaag gcgatcatt 
      acgggcctg agtaacgtc tacacgagc tacataaca 
      acgccggag gaataccaa atgcagcga tatgaggga 
      gcttccact atttacggg cctcacacg cttaccatt 
      tacatcgaa aaatacaaa cagctgact aaagctctc 
      ctcaaagga gaaaagctc gactccggg cctttacct 
      gccgacctt tcccagcga gttatttca ctacaaccc 
      agggttatg ttcgatact ccaggtctg atgcatcat 
      ttcggcgat tgtttactc cagcccccg aaaagcgca 
      agagtggga agcaaagtt cgagcgaaa ttcgtatct 
      ggccaccct cgtaataac ctaatgacc gagaaaact 
      ttcctcact gttgaaaaa ttagcagac aatggcaag 
      tggatcgtc cttgccacc gacgcggat tgggaaaca 
      aagtttact tggacgaga acttcaacg ctgattggt 
      ggaagtgag gcaacaatt gagtgggaa attccttcc 
      gacgcaaca ccgggactt taccgaata caccacttt 
      ggacacttc caacatatt tttggcggg gttcaccca 
      tacagcggt gcaactgat actttccaa gtcactaag 
      tga
    mRNA sequence
    • >TC011170 RA
      agcaccagt ggcggaagt acttatcat tgacatcca 
      aacgcttgt cattgtcag tgttaggtt tctttgttt 
      acgtgcaac taataaacg atgacttaa accaaaacc 
      gtacattga cacccatcg attttttta ttatttaaa 
      tttgaaaat tgaaaattc gtacgtaag tactcgtaa 
      atttatcgt cacatgaaa tataaacac ttgttatca 
      ccgatttaa gtggcacaa gatgacttc tgtgtagat 
      actttcact ctgttacgt catacgtga tctgaagag 
      cgcgagcta accgttata ttttttttt cgatttatt 
      ctaatagat gtttatggt gagtggatc aatatcaag 
      gcaaaattc ttcggaagt gttgagtca accatgaca 
      gtgagttac accagcgaa gagtggtgt ttatcaaac 
      gtaatttgg tgcacgaca gtgttggac gaagtttta 
      ccggaaaaa ttcgaagcc tggaaaatt tttaaacgc 
      cccatattg tgaccaagg agtgtaaaa tgcgaaaaa 
      agctcttag tctggatta taacattat cgacatgtc 
      tccgcacga ctttggggc tctacaaaa ttggggtcg 
      gaatagccg actgtacgg gaccggcag ctgaagtca 
      catttatgg gttatggaa aatcgaatc agaagggtt 
      gcgggatac atttgcgtc aattttctc gcgcctttg 
      tcatcgatg atgggtctc agagggctg ctttcgtta 
      gcgtggacg ctggaatga tggcctatg gggtccgca 
      aggctgtca tttctcgcc tcggcaagc aatacaaag 
      acgtctaca ctgagagta acctaattt tgagcggga 
      cacacactc acagcgctc ccggaggat ttctgatgg 
      attttatgt atgatattc caagtttgg gatttgtga 
      aagaaacgt ttgacgctc ttgttgatg gaatcacga 
      ggtctgttg tcagggcgc atgagaata tggtagatg 
      ggaagatat ttatcacga caggggagt tgattggag 
      cgaatatta atagaagtc caatgtcct atttgctga 
      atcctaagg aggaacgtg aaaaataca aatacaatg 
      tggacaaag aaatggtgc agctgaagc tcgtcaact 
      ccgccaact cgctcatcg gcgtcatca gctggtttc 
      ccgtccatc ccacgtcca tgaacaaca caaactgcc 
      taatcacct ccgataatg tgggctacg cgtccgtcc 
      tgctcgaaa aatctctga ataaaaatt cgctcgttg 
      gccaaggac ggattgtgg cggctttcg cgtccacca 
      atttagggg acgtttcac cgaacacca aagggccga 
      ggtgtgtga cgacggggg aggaatgcg atgttgtgt 
      cgagtacgt gcgacggac aagcaaagt tttgtgtgg 
      cggcaggac cgggcgagg atatgtttg agagtacga 
      aaataatcg cagagaaac tttttgaaa aatcgaaga 
      ttcttttac aatcctctg aggcgaaag aggtgaccg 
      gtcctgtca gacacattc accaatacg tggacatgc 
      cgtctcaaa acgtgacca tcacactac ccaacggta 
      ccataaccc atgtcactg ggtgcctcc cagcgatgg 
      gttacagct tcgctgctg gtaccaccg acggagtgg 
      gcgagtttg gctttgccc aaggcacca gatcgacaa 
      actcccatt gggacactc tgcgagact tgctgttca 
      agccgacgg aagaagaca tcaggtgcc agcatccta 
      aaccaattt taatcaacg ccggaagga tttccatcc 
      cttatcagt ggcaaccgc ggattgttt ccacgcaaa 
      tcgtccaag tgggtgaag tgcttctaa ttgccgttc 
      ccggcgaat tcaccacaa tgtctggca gacgtctcc 
      gaaatggcg tgcaggaaa ccgccgtca gaagcgggg 
      cttcccctg gcacaaagg cgatcatta cgggcctga 
      gtaacgtct acacgagct acataacaa cgccggagg 
      aataccaaa tgcagcgat atgagggag cttccacta 
      tttacgggc ctcacacgc ttaccattt acatcgaaa 
      aatacaaac agctgacta aagctctcc tcaaaggag 
      aaaagctcg actccgggc ctttacctg ccgaccttt 
      cccagcgag ttatttcac tacaaccca gggttatgt 
      tcgatactc caggtctga tgcatcatt tcggcgatt 
      gtttactcc agcccccga aaagcgcaa gagtgggaa 
      gcaaagttc gagcgaaat tcgtatctg gccaccctc 
      gtaataacc taatgaccg agaaaactt tcctcactg 
      ttgaaaaat tagcagaca atggcaagt ggatcgtcc 
      ttgccaccg acgcggatt gggaaacaa agtttactt 
      ggacgagaa cttcaacgc tgattggtg gaagtgagg 
      caacaattg agtgggaaa ttccttccg acgcaacac 
      cgggacttt accgaatac accactttg gacacttcc 
      aacatattt ttggcgggg ttcacccat acagcggtg 
      caactgata ctttccaag tcactaagt gattgtaag 
      actgacaag tactctagt taaaatgta ttatatttc 
      ttgccattg tagatcaat aaagttgtt ataagaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY