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Gene information
General information
TC011170 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 3350858..3372872
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011170
    iBeetle screen iB_04979

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011170 RA
      MRKKLLVWI ITLSTCLRT TLGLYKIGV GIADCTGPA 
AEVTFMGYG KSNQKGCGI HLRQFSRAF VIDDGSQRA 
AFVSVDAGM MAYGVRKAV ISRLGKQYK DVYTESNLI 
LSGTHTHSA PGGFLMDFM YDIPSLGFV KETFDALVD 
GITRSVVRA HENMVDGKI FITTGELIG ANINRSPMS 
YLLNPKEER EKYKYNVDK EMVQLKLVN SANSLIGVI 
SWFPVHPTS MNNTNCLIT SDNVGYASV LLEKSLNKN 
SLVGQGRIV AAFASTNLG DVSPNTKGP RCVTTGEEC 
DVVSSTCDG QAKFCVAAG PGEDMFEST KIIAEKLFE 
KSKILLQSS EAKEVTGPV RHIHQYVDM PSQNVTITL 
PNGTITHVT GCLPAMGYS FAAGTTDGV GEFGFAQGT 
RSTNSHWDT LRDLLFKPT EEDIRCQHP KPILINAGR 
ISIPYQWQP RIVSTQIVQ VGEVLLIAV PGEFTTMSG 
RRLRNGVQE TAVRSGASP GTKAIITGL SNVYTSYIT 
TPEEYQMQR YEGASTIYG PHTLTIYIE KYKQLTKAL 
LKGEKLDSG PLPADLSQR VISLQPRVM FDTPGLMHH 
FGDCLLQPP KSARVGSKV RAKFVSGHP RNNLMTEKT 
FLTVEKLAD NGKWIVLAT DADWETKFT WTRTSTLIG 
GSEATIEWE IPSDATPGL YRIHHFGHF QHIFGGVHP 
YSGATDTFQ VTK
    CDS sequence
    • >TC011170 RA
      atgcgaaaa aagctctta gtctggatt ataacatta 
tcgacatgt ctccgcacg actttgggg ctctacaaa 
attggggtc ggaatagcc gactgtacg ggaccggca 
gctgaagtc acatttatg ggttatgga aaatcgaat 
cagaagggt tgcgggata catttgcgt caattttct 
cgcgccttt gtcatcgat gatgggtct cagagggct 
gctttcgtt agcgtggac gctggaatg atggcctat 
ggggtccgc aaggctgtc atttctcgc ctcggcaag 
caatacaaa gacgtctac actgagagt aacctaatt 
ttgagcggg acacacact cacagcgct cccggagga 
tttctgatg gattttatg tatgatatt ccaagtttg 
ggatttgtg aaagaaacg tttgacgct cttgttgat 
ggaatcacg aggtctgtt gtcagggcg catgagaat 
atggtagat gggaagata tttatcacg acaggggag 
ttgattgga gcgaatatt aatagaagt ccaatgtcc 
tatttgctg aatcctaag gaggaacgt gaaaaatac 
aaatacaat gtggacaaa gaaatggtg cagctgaag 
ctcgtcaac tccgccaac tcgctcatc ggcgtcatc 
agctggttt cccgtccat cccacgtcc atgaacaac 
acaaactgc ctaatcacc tccgataat gtgggctac 
gcgtccgtc ctgctcgaa aaatctctg aataaaaat 
tcgctcgtt ggccaagga cggattgtg gcggctttc 
gcgtccacc aatttaggg gacgtttca ccgaacacc 
aaagggccg aggtgtgtg acgacgggg gaggaatgc 
gatgttgtg tcgagtacg tgcgacgga caagcaaag 
ttttgtgtg gcggcagga ccgggcgag gatatgttt 
gagagtacg aaaataatc gcagagaaa ctttttgaa 
aaatcgaag attctttta caatcctct gaggcgaaa 
gaggtgacc ggtcctgtc agacacatt caccaatac 
gtggacatg ccgtctcaa aacgtgacc atcacacta 
cccaacggt accataacc catgtcact gggtgcctc 
ccagcgatg ggttacagc ttcgctgct ggtaccacc 
gacggagtg ggcgagttt ggctttgcc caaggcacc 
agatcgaca aactcccat tgggacact ctgcgagac 
ttgctgttc aagccgacg gaagaagac atcaggtgc 
cagcatcct aaaccaatt ttaatcaac gccggaagg 
atttccatc ccttatcag tggcaaccg cggattgtt 
tccacgcaa atcgtccaa gtgggtgaa gtgcttcta 
attgccgtt cccggcgaa ttcaccaca atgtctggc 
agacgtctc cgaaatggc gtgcaggaa accgccgtc 
agaagcggg gcttcccct ggcacaaag gcgatcatt 
acgggcctg agtaacgtc tacacgagc tacataaca 
acgccggag gaataccaa atgcagcga tatgaggga 
gcttccact atttacggg cctcacacg cttaccatt 
tacatcgaa aaatacaaa cagctgact aaagctctc 
ctcaaagga gaaaagctc gactccggg cctttacct 
gccgacctt tcccagcga gttatttca ctacaaccc 
agggttatg ttcgatact ccaggtctg atgcatcat 
ttcggcgat tgtttactc cagcccccg aaaagcgca 
agagtggga agcaaagtt cgagcgaaa ttcgtatct 
ggccaccct cgtaataac ctaatgacc gagaaaact 
ttcctcact gttgaaaaa ttagcagac aatggcaag 
tggatcgtc cttgccacc gacgcggat tgggaaaca 
aagtttact tggacgaga acttcaacg ctgattggt 
ggaagtgag gcaacaatt gagtgggaa attccttcc 
gacgcaaca ccgggactt taccgaata caccacttt 
ggacacttc caacatatt tttggcggg gttcaccca 
tacagcggt gcaactgat actttccaa gtcactaag 
tga
    mRNA sequence
    • >TC011170 RA
      agcaccagt ggcggaagt acttatcat tgacatcca 
aacgcttgt cattgtcag tgttaggtt tctttgttt 
acgtgcaac taataaacg atgacttaa accaaaacc 
gtacattga cacccatcg attttttta ttatttaaa 
tttgaaaat tgaaaattc gtacgtaag tactcgtaa 
atttatcgt cacatgaaa tataaacac ttgttatca 
ccgatttaa gtggcacaa gatgacttc tgtgtagat 
actttcact ctgttacgt catacgtga tctgaagag 
cgcgagcta accgttata ttttttttt cgatttatt 
ctaatagat gtttatggt gagtggatc aatatcaag 
gcaaaattc ttcggaagt gttgagtca accatgaca 
gtgagttac accagcgaa gagtggtgt ttatcaaac 
gtaatttgg tgcacgaca gtgttggac gaagtttta 
ccggaaaaa ttcgaagcc tggaaaatt tttaaacgc 
cccatattg tgaccaagg agtgtaaaa tgcgaaaaa 
agctcttag tctggatta taacattat cgacatgtc 
tccgcacga ctttggggc tctacaaaa ttggggtcg 
gaatagccg actgtacgg gaccggcag ctgaagtca 
catttatgg gttatggaa aatcgaatc agaagggtt 
gcgggatac atttgcgtc aattttctc gcgcctttg 
tcatcgatg atgggtctc agagggctg ctttcgtta 
gcgtggacg ctggaatga tggcctatg gggtccgca 
aggctgtca tttctcgcc tcggcaagc aatacaaag 
acgtctaca ctgagagta acctaattt tgagcggga 
cacacactc acagcgctc ccggaggat ttctgatgg 
attttatgt atgatattc caagtttgg gatttgtga 
aagaaacgt ttgacgctc ttgttgatg gaatcacga 
ggtctgttg tcagggcgc atgagaata tggtagatg 
ggaagatat ttatcacga caggggagt tgattggag 
cgaatatta atagaagtc caatgtcct atttgctga 
atcctaagg aggaacgtg aaaaataca aatacaatg 
tggacaaag aaatggtgc agctgaagc tcgtcaact 
ccgccaact cgctcatcg gcgtcatca gctggtttc 
ccgtccatc ccacgtcca tgaacaaca caaactgcc 
taatcacct ccgataatg tgggctacg cgtccgtcc 
tgctcgaaa aatctctga ataaaaatt cgctcgttg 
gccaaggac ggattgtgg cggctttcg cgtccacca 
atttagggg acgtttcac cgaacacca aagggccga 
ggtgtgtga cgacggggg aggaatgcg atgttgtgt 
cgagtacgt gcgacggac aagcaaagt tttgtgtgg 
cggcaggac cgggcgagg atatgtttg agagtacga 
aaataatcg cagagaaac tttttgaaa aatcgaaga 
ttcttttac aatcctctg aggcgaaag aggtgaccg 
gtcctgtca gacacattc accaatacg tggacatgc 
cgtctcaaa acgtgacca tcacactac ccaacggta 
ccataaccc atgtcactg ggtgcctcc cagcgatgg 
gttacagct tcgctgctg gtaccaccg acggagtgg 
gcgagtttg gctttgccc aaggcacca gatcgacaa 
actcccatt gggacactc tgcgagact tgctgttca 
agccgacgg aagaagaca tcaggtgcc agcatccta 
aaccaattt taatcaacg ccggaagga tttccatcc 
cttatcagt ggcaaccgc ggattgttt ccacgcaaa 
tcgtccaag tgggtgaag tgcttctaa ttgccgttc 
ccggcgaat tcaccacaa tgtctggca gacgtctcc 
gaaatggcg tgcaggaaa ccgccgtca gaagcgggg 
cttcccctg gcacaaagg cgatcatta cgggcctga 
gtaacgtct acacgagct acataacaa cgccggagg 
aataccaaa tgcagcgat atgagggag cttccacta 
tttacgggc ctcacacgc ttaccattt acatcgaaa 
aatacaaac agctgacta aagctctcc tcaaaggag 
aaaagctcg actccgggc ctttacctg ccgaccttt 
cccagcgag ttatttcac tacaaccca gggttatgt 
tcgatactc caggtctga tgcatcatt tcggcgatt 
gtttactcc agcccccga aaagcgcaa gagtgggaa 
gcaaagttc gagcgaaat tcgtatctg gccaccctc 
gtaataacc taatgaccg agaaaactt tcctcactg 
ttgaaaaat tagcagaca atggcaagt ggatcgtcc 
ttgccaccg acgcggatt gggaaacaa agtttactt 
ggacgagaa cttcaacgc tgattggtg gaagtgagg 
caacaattg agtgggaaa ttccttccg acgcaacac 
cgggacttt accgaatac accactttg gacacttcc 
aacatattt ttggcgggg ttcacccat acagcggtg 
caactgata ctttccaag tcactaagt gattgtaag 
actgacaag tactctagt taaaatgta ttatatttc 
ttgccattg tagatcaat aaagttgtt ataagaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY