| General information | |||
| TC011170 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007425.3: 3350858..3372872 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC011170 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04979 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
- >TC011170 RA
MRKKLLVWI ITLSTCLRT TLGLYKIGV GIADCTGPA AEVTFMGYG KSNQKGCGI HLRQFSRAF VIDDGSQRA AFVSVDAGM MAYGVRKAV ISRLGKQYK DVYTESNLI LSGTHTHSA PGGFLMDFM YDIPSLGFV KETFDALVD GITRSVVRA HENMVDGKI FITTGELIG ANINRSPMS YLLNPKEER EKYKYNVDK EMVQLKLVN SANSLIGVI SWFPVHPTS MNNTNCLIT SDNVGYASV LLEKSLNKN SLVGQGRIV AAFASTNLG DVSPNTKGP RCVTTGEEC DVVSSTCDG QAKFCVAAG PGEDMFEST KIIAEKLFE KSKILLQSS EAKEVTGPV RHIHQYVDM PSQNVTITL PNGTITHVT GCLPAMGYS FAAGTTDGV GEFGFAQGT RSTNSHWDT LRDLLFKPT EEDIRCQHP KPILINAGR ISIPYQWQP RIVSTQIVQ VGEVLLIAV PGEFTTMSG RRLRNGVQE TAVRSGASP GTKAIITGL SNVYTSYIT TPEEYQMQR YEGASTIYG PHTLTIYIE KYKQLTKAL LKGEKLDSG PLPADLSQR VISLQPRVM FDTPGLMHH FGDCLLQPP KSARVGSKV RAKFVSGHP RNNLMTEKT FLTVEKLAD NGKWIVLAT DADWETKFT WTRTSTLIG GSEATIEWE IPSDATPGL YRIHHFGHF QHIFGGVHP YSGATDTFQ VTK
- >TC011170 RA
atgcgaaaa aagctctta gtctggatt ataacatta tcgacatgt ctccgcacg actttgggg ctctacaaa attggggtc ggaatagcc gactgtacg ggaccggca gctgaagtc acatttatg ggttatgga aaatcgaat cagaagggt tgcgggata catttgcgt caattttct cgcgccttt gtcatcgat gatgggtct cagagggct gctttcgtt agcgtggac gctggaatg atggcctat ggggtccgc aaggctgtc atttctcgc ctcggcaag caatacaaa gacgtctac actgagagt aacctaatt ttgagcggg acacacact cacagcgct cccggagga tttctgatg gattttatg tatgatatt ccaagtttg ggatttgtg aaagaaacg tttgacgct cttgttgat ggaatcacg aggtctgtt gtcagggcg catgagaat atggtagat gggaagata tttatcacg acaggggag ttgattgga gcgaatatt aatagaagt ccaatgtcc tatttgctg aatcctaag gaggaacgt gaaaaatac aaatacaat gtggacaaa gaaatggtg cagctgaag ctcgtcaac tccgccaac tcgctcatc ggcgtcatc agctggttt cccgtccat cccacgtcc atgaacaac acaaactgc ctaatcacc tccgataat gtgggctac gcgtccgtc ctgctcgaa aaatctctg aataaaaat tcgctcgtt ggccaagga cggattgtg gcggctttc gcgtccacc aatttaggg gacgtttca ccgaacacc aaagggccg aggtgtgtg acgacgggg gaggaatgc gatgttgtg tcgagtacg tgcgacgga caagcaaag ttttgtgtg gcggcagga ccgggcgag gatatgttt gagagtacg aaaataatc gcagagaaa ctttttgaa aaatcgaag attctttta caatcctct gaggcgaaa gaggtgacc ggtcctgtc agacacatt caccaatac gtggacatg ccgtctcaa aacgtgacc atcacacta cccaacggt accataacc catgtcact gggtgcctc ccagcgatg ggttacagc ttcgctgct ggtaccacc gacggagtg ggcgagttt ggctttgcc caaggcacc agatcgaca aactcccat tgggacact ctgcgagac ttgctgttc aagccgacg gaagaagac atcaggtgc cagcatcct aaaccaatt ttaatcaac gccggaagg atttccatc ccttatcag tggcaaccg cggattgtt tccacgcaa atcgtccaa gtgggtgaa gtgcttcta attgccgtt cccggcgaa ttcaccaca atgtctggc agacgtctc cgaaatggc gtgcaggaa accgccgtc agaagcggg gcttcccct ggcacaaag gcgatcatt acgggcctg agtaacgtc tacacgagc tacataaca acgccggag gaataccaa atgcagcga tatgaggga gcttccact atttacggg cctcacacg cttaccatt tacatcgaa aaatacaaa cagctgact aaagctctc ctcaaagga gaaaagctc gactccggg cctttacct gccgacctt tcccagcga gttatttca ctacaaccc agggttatg ttcgatact ccaggtctg atgcatcat ttcggcgat tgtttactc cagcccccg aaaagcgca agagtggga agcaaagtt cgagcgaaa ttcgtatct ggccaccct cgtaataac ctaatgacc gagaaaact ttcctcact gttgaaaaa ttagcagac aatggcaag tggatcgtc cttgccacc gacgcggat tgggaaaca aagtttact tggacgaga acttcaacg ctgattggt ggaagtgag gcaacaatt gagtgggaa attccttcc gacgcaaca ccgggactt taccgaata caccacttt ggacacttc caacatatt tttggcggg gttcaccca tacagcggt gcaactgat actttccaa gtcactaag tga
- >TC011170 RA
agcaccagt ggcggaagt acttatcat tgacatcca aacgcttgt cattgtcag tgttaggtt tctttgttt acgtgcaac taataaacg atgacttaa accaaaacc gtacattga cacccatcg attttttta ttatttaaa tttgaaaat tgaaaattc gtacgtaag tactcgtaa atttatcgt cacatgaaa tataaacac ttgttatca ccgatttaa gtggcacaa gatgacttc tgtgtagat actttcact ctgttacgt catacgtga tctgaagag cgcgagcta accgttata ttttttttt cgatttatt ctaatagat gtttatggt gagtggatc aatatcaag gcaaaattc ttcggaagt gttgagtca accatgaca gtgagttac accagcgaa gagtggtgt ttatcaaac gtaatttgg tgcacgaca gtgttggac gaagtttta ccggaaaaa ttcgaagcc tggaaaatt tttaaacgc cccatattg tgaccaagg agtgtaaaa tgcgaaaaa agctcttag tctggatta taacattat cgacatgtc tccgcacga ctttggggc tctacaaaa ttggggtcg gaatagccg actgtacgg gaccggcag ctgaagtca catttatgg gttatggaa aatcgaatc agaagggtt gcgggatac atttgcgtc aattttctc gcgcctttg tcatcgatg atgggtctc agagggctg ctttcgtta gcgtggacg ctggaatga tggcctatg gggtccgca aggctgtca tttctcgcc tcggcaagc aatacaaag acgtctaca ctgagagta acctaattt tgagcggga cacacactc acagcgctc ccggaggat ttctgatgg attttatgt atgatattc caagtttgg gatttgtga aagaaacgt ttgacgctc ttgttgatg gaatcacga ggtctgttg tcagggcgc atgagaata tggtagatg ggaagatat ttatcacga caggggagt tgattggag cgaatatta atagaagtc caatgtcct atttgctga atcctaagg aggaacgtg aaaaataca aatacaatg tggacaaag aaatggtgc agctgaagc tcgtcaact ccgccaact cgctcatcg gcgtcatca gctggtttc ccgtccatc ccacgtcca tgaacaaca caaactgcc taatcacct ccgataatg tgggctacg cgtccgtcc tgctcgaaa aatctctga ataaaaatt cgctcgttg gccaaggac ggattgtgg cggctttcg cgtccacca atttagggg acgtttcac cgaacacca aagggccga ggtgtgtga cgacggggg aggaatgcg atgttgtgt cgagtacgt gcgacggac aagcaaagt tttgtgtgg cggcaggac cgggcgagg atatgtttg agagtacga aaataatcg cagagaaac tttttgaaa aatcgaaga ttcttttac aatcctctg aggcgaaag aggtgaccg gtcctgtca gacacattc accaatacg tggacatgc cgtctcaaa acgtgacca tcacactac ccaacggta ccataaccc atgtcactg ggtgcctcc cagcgatgg gttacagct tcgctgctg gtaccaccg acggagtgg gcgagtttg gctttgccc aaggcacca gatcgacaa actcccatt gggacactc tgcgagact tgctgttca agccgacgg aagaagaca tcaggtgcc agcatccta aaccaattt taatcaacg ccggaagga tttccatcc cttatcagt ggcaaccgc ggattgttt ccacgcaaa tcgtccaag tgggtgaag tgcttctaa ttgccgttc ccggcgaat tcaccacaa tgtctggca gacgtctcc gaaatggcg tgcaggaaa ccgccgtca gaagcgggg cttcccctg gcacaaagg cgatcatta cgggcctga gtaacgtct acacgagct acataacaa cgccggagg aataccaaa tgcagcgat atgagggag cttccacta tttacgggc ctcacacgc ttaccattt acatcgaaa aatacaaac agctgacta aagctctcc tcaaaggag aaaagctcg actccgggc ctttacctg ccgaccttt cccagcgag ttatttcac tacaaccca gggttatgt tcgatactc caggtctga tgcatcatt tcggcgatt gtttactcc agcccccga aaagcgcaa gagtgggaa gcaaagttc gagcgaaat tcgtatctg gccaccctc gtaataacc taatgaccg agaaaactt tcctcactg ttgaaaaat tagcagaca atggcaagt ggatcgtcc ttgccaccg acgcggatt gggaaacaa agtttactt ggacgagaa cttcaacgc tgattggtg gaagtgagg caacaattg agtgggaaa ttccttccg acgcaacac cgggacttt accgaatac accactttg gacacttcc aacatattt ttggcgggg ttcacccat acagcggtg caactgata ctttccaag tcactaagt gattgtaag actgacaag tactctagt taaaatgta ttatatttc ttgccattg tagatcaat aaagttgtt ataagaat
