Status
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Gene information
General information
TC011169 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 3374821..3381762
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011169
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04978

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011169 RA
      MSGRRLRDA VKETIISNG SSENTRPII TGLSNVYTN 
      YIATPEEYQ GDSLDLGPT PPDFFSQLI TLVSPPSVD 
      GIAQGQHFG DCLQQPPES VSIGDTVSV KSVQGCLPA 
      VGYAFGAGT TDGPGDSLF SQATQTSNP FWNFVRDFV 
      SVPTKDDVS CHDPKPIML NTGRASLPY DWQPKIVPT 
      QIVTIGEVV LIAVPGEFT TMAGRRLRE AVAGNPRHD 
      SLRGVTFLT VKKLIGKDW TVVLTDANW ETKYHWERN 
      KLTKQNTAT IEWSIKDDY TAKLCYGVN PRLLESYIV 
      GKSGLKITL KSGPNMIKI LALLTFFCY VSADYQVGI 
      GRADCTGPP AEIIFMGYA KLSQVGCGL HLRQFARAY 
      IIDDGTSRA AFVTVDALA MAHSVRRQV LKNLAELYN 
      DTYTEQNLI ISGTHTHST PGGWFMDLM LDIPVLGFV 
      QESFDTLVE GITQAITNA HENMVEAKI FLSSGILLD 
      ANINRSPAS YLYNPEEER AKYEYNVDK EMVQLKFVR 
      SSDTQPIGV INWFAVHPT SMNNTNCLL TSDNVGYAS 
      LLLEDHVNN GTLPGKGSF VGAFASTNL GDVSPNTKG 
      PICIDTGEE CDFITSTCN GNAQTCIAS GPGNNMAES 
      TQIIAGKLF NKAKELFHQ EDTFEVTGP VKFIHQYVD 
      MPNEKATIT LANGTQQEI RGCLPAMGY SFAGGTTDG 
      PGEFDFAQG TTTDNPFWD AVRDFVFPP TEDEIACHA 
      PKPILINTG GVDFPYPWQ PRIVTTQIV TVGEVALIA 
      VPGEFTTMS GRRLRDAVK ETIISNGGS ENTRPIITG 
      LSNVYTNYI ATPEEYQLQ RYEGGSTIY GPQTLNLYI 
      NKFKQLATA LMKGETLDS GPTPPDFSS QLITLVSPP 
      SVDGIAQGQ NFGDCLQQP PESVSIGDT VSVKFVSGN 
      PRHDVLHGG TYISVEKSE GDDWVLVAS DANWETRFH 
      WKRTNVLTG ESEVTIEWD IGQDVVPGQ YRIRHNGHY 
      KEKSGEIHA YEGITSTFK IA
    CDS sequence
    • >TC011169 RA
      atgtcaggt cggaggctg agagacgca gtcaaagaa 
      acaataatt agtaatggt agttccgaa aacactcga 
      ccaattatt acaggtctt agtaacgtc tatactaac 
      tatattgcg actccggag gagtaccaa ggagacagt 
      ctagatcta ggaccaact cctccagat ttcttctcc 
      cagttgatc actttagtt tcaccacct tcagttgat 
      ggaattgca caaggccaa cattttggc gactgtctc 
      caacaacca cccgaatcc gtttcaatc ggagacact 
      gtttcagta aaatctgtt caaggttgt ctcccagca 
      gttgggtac gcttttggt gctggtacc actgatggt 
      cctggtgat tcattgttt agtcaagcg acacagacc 
      agtaacccg ttttggaat tttgtgaga gactttgtg 
      tcagtaccg acgaaagac gatgtttcc tgtcatgac 
      cctaagccc attatgctt aatacagga cgggctagt 
      cttccgtac gattggcaa cctaaaata gtaccaaca 
      caaatagtt acaattgga gaagtggta cttatagca 
      gtaccggga gaattcaca acaatggcc ggaagaaga 
      ctacgagaa gcggttgct ggtaaccct agacatgat 
      tcgttacgt ggtgttact tttcttaca gtgaaaaaa 
      ttaattggc aaggattgg acggtggta ctcactgat 
      gcgaactgg gagacaaag taccactgg gagagaaat 
      aagctcaca aaacaaaat acggcaact attgagtgg 
      agtattaag gacgattac acagctaaa ctttgctac 
      ggggttaat ccaagatta ctagaatct tatatagtg 
      ggcaaaagt ggcctcaaa atcacattg aaaagtggc 
      cccaacatg attaaaatt ttagctttg ttaacgttt 
      ttctgctat gtttcagcc gattatcag gttgggatt 
      ggacgagcg gattgtaca ggacctcca gctgagata 
      atctttatg ggctatgct aaattaagt caagtgggt 
      tgtgggcta catttacga caatttgca agagcttat 
      attattgat gatgggaca agtcgagcc gcttttgtg 
      actgtagat gctcttgca atggcacat tcagtacgg 
      agacaggtt ttaaaaaat ctggctgaa ctttataat 
      gatacgtac actgagcaa aatttgata ataagtggt 
      actcacacc cactcgacc cctggcggt tggtttatg 
      gatttgatg ttggatatc ccagttttg ggttttgtt 
      caggagagc ttcgacaca cttgttgag ggaatcact 
      caagctata acaaacgct cacgaaaac atggtcgaa 
      gctaagata tttttatcg agtggaatt ctcctggat 
      gcaaatatt aatcgaagt ccagcttcg tatttatat 
      aatcctgaa gaggagcgc gcaaagtat gagtacaat 
      gttgataaa gaaatggtt cagctaaaa ttcgttcgt 
      tcatctgac acgcaaccg atcggtgta atcaattgg 
      ttcgccgtc catccgaca tccatgaac aatacaaac 
      tgcttgctc acgtctgat aacgtcgga tatgcctcg 
      cttttactc gaagaccat gtcaataac ggcaccctt 
      ccgggaaaa ggatcattt gttggagct tttgcttcc 
      accaactta ggggatgtt tccccaaac actaaaggc 
      ccgatttgt atcgacaca ggcgaagaa tgcgatttt 
      attacaagc acttgtaat ggcaacgct caaacgtgt 
      atcgcttca gggcctggg aataacatg gctgagagc 
      acacaaatc atcgctgga aaacttttc aataaagcg 
      aaagagttg ttccatcaa gaagacact tttgaagtc 
      actggacct gttaaattc attcatcaa tatgtcgat 
      atgccaaat gaaaaagct acaattact ttggctaat 
      gggacgcaa caagaaatc cggggttgt ctcccagct 
      atgggttac agttttgcc ggtggtacc actgatggt 
      cccggagaa ttcgatttt gcccaaggt accaccact 
      gataatccc ttctgggac gctgttaga gattttgtt 
      tttccacca actgaagat gagattgct tgtcacgct 
      cctaaaccc attctgatt aatactgga ggcgtcgat 
      tttccatac ccttggcaa cccagaatt gttacaact 
      caaattgtt actgttgga gaagttgcg ctaattgct 
      gttcctgga gagtttacc acaatgtca ggtcggaga 
      ttaagagac gcagtcaaa gaaacgata attagtaat 
      ggtggttcc gaaaacact cgaccaatt atcacaggt 
      cttagtaac gtctatact aactatatt gcgactccg 
      gaggagtac caattgcaa cggtacgaa ggtggttcc 
      accatttat ggtccacaa accctaaac ctctacata 
      aataaattc aaacaactg gccacagct ttaatgaag 
      ggagaaact ctagattca ggaccaact cctccagat 
      ttctcctcc cagttgatc actttagtt tcaccacct 
      tcagttgat ggaattgca caaggccaa aattttggc 
      gactgtctc caacaacca cccgaatcc gtttcaatc 
      ggagacact gtttcagta aaatttgtg agtggcaac 
      cctcggcat gatgttctc cacggtggg acatatatt 
      agtgtggaa aaatcggaa ggtgatgac tgggttctt 
      gtggcttcg gatgcaaac tgggaaaca cggtttcat 
      tggaagagg acaaacgta ttaacggga gagtctgag 
      gttaccatt gaatgggat attggtcaa gatgtggta 
      ccagggcag taccgaata agacataat ggacattat 
      aaagaaaaa tctggagag attcacgct tatgaaggc 
      ataacgagt acatttaaa attgcctga
    mRNA sequence
    • >TC011169 RA
      aaattagga gttgttcca tcaagaaga cacttttga 
      agtcactgg acctgtcaa attcattca tcaatatgt 
      agatatgct gaatgaaaa agctacaat tactttggc 
      taatgggac gcaacaaga agtgagtca acttgtttt 
      ggtcaaaat ttattgtaa attttagat ccgaggttg 
      tcccccagc catgggtta cagttttgc cggtgatac 
      cactgatgg tcccggaga attcgattt tgcgcaagg 
      taccaccac tgataatcc cttctgaga cgctgttag 
      agattttgt ttttccacc aactgaaga tgagattgc 
      ttgtcacgc tcctaaacc cattctgat taatactgg 
      aggcgttga ttttccata cccttggca acccagaat 
      tgtaacaac tcaaattgt tactgttgg agaagttgc 
      gctaattgc tgttcctgg agagtttac cacaatgtc 
      aggtcggag gctgagaga cgcagtcaa agaaacaat 
      aattagtaa tggtagttc cgaaaacac tcgaccaat 
      tattacagg tcttagtaa cgtctatac taactatat 
      tgcgactcc ggaggagta ccaaggaga cagtctaga 
      tctaggacc aactcctcc agatttctt ctcccagtt 
      gatcacttt agtttcacc accttcagt tgatggaat 
      tgcacaagg ccaacattt tggcgactg tctccaaca 
      accacccga atccgtttc aatcggaga cactgtttc 
      agtaaaatc tgttcaagg ttgtctccc agcagttgg 
      gtacgcttt tggtgctgg taccactga tggtcctgg 
      tgattcatt gtttagtca agcgacaca gaccagtaa 
      cccgttttg gaattttgt gagagactt tgtgtcagt 
      accgacgaa agacgatgt ttcctgtca tgaccctaa 
      gcccattat gcttaatac aggacgggc tagtcttcc 
      gtacgattg gcaacctaa aatagtacc aacacaaat 
      agttacaat tggagaagt ggtacttat agcagtacc 
      gggagaatt cacaacaat ggccggaag aagactacg 
      agaagcggt tgctggtaa ccctagaca tgattcgtt 
      acgtggtgt tacttttct tacagtgaa aaaattaat 
      tggcaagga ttggacggt ggtactcac tgatgcgaa 
      ctgggagac aaagtacca ctgggagag aaataagct 
      cacaaaaca aaatacggc aactattga gtggagtat 
      taaggacga ttacacagc taaactttg ctacggggt 
      taatccaag attactaga atcttatat agtgggcaa 
      aagtggcct caaaatcac attgaaaag tggccccaa 
      catgattaa aattttagc tttgttaac gtttttctg 
      ctatgtttc agccgatta tcaggttgg gattggacg 
      agcggattg tacaggacc tccagctga gataatctt 
      tatgggcta tgctaaatt aagtcaagt gggttgtgg 
      gctacattt acgacaatt tgcaagagc ttatattat 
      tgatgatgg gacaagtcg agccgcttt tgtgactgt 
      agatgctct tgcaatggc acattcagt acggagaca 
      ggttttaaa aaatctggc tgaacttta taatgatac 
      gtacactga gcaaaattt gataataag tggtactca 
      cacccactc gacccctgg cggttggtt tatggattt 
      gatgttgga tatcccagt tttgggttt tgttcagga 
      gagcttcga cacacttgt tgagggaat cactcaagc 
      tataacaaa cgctcacga aaacatggt cgaagctaa 
      gatattttt atcgagtgg aattctcct ggatgcaaa 
      tattaatcg aagtccagc ttcgtattt atataatcc 
      tgaagagga gcgcgcaaa gtatgagta caatgttga 
      taaagaaat ggttcagct aaaattcgt tcgttcatc 
      tgacacgca accgatcgg tgtaatcaa ttggttcgc 
      cgtccatcc gacatccat gaacaatac aaactgctt 
      gctcacgtc tgataacgt cggatatgc ctcgctttt 
      actcgaaga ccatgtcaa taacggcac ccttccggg 
      aaaaggatc atttgttgg agcttttgc ttccaccaa 
      cttagggga tgtttcccc aaacactaa aggcccgat 
      ttgtatcga cacaggcga agaatgcga ttttattac 
      aagcacttg taatggcaa cgctcaaac gtgtatcgc 
      ttcagggcc tgggaataa catggctga gagcacaca 
      aatcatcgc tggaaaact tttcaataa agcgaaaga 
      gttgttcca tcaagaaga cacttttga agtcactgg 
      acctgttaa attcattca tcaatatgt cgatatgcc 
      aaatgaaaa agctacaat tactttggc taatgggac 
      gcaacaaga aatccgggg ttgtctccc agctatggg 
      ttacagttt tgccggtgg taccactga tggtcccgg 
      agaattcga ttttgccca aggtaccac cactgataa 
      tcccttctg ggacgctgt tagagattt tgtttttcc 
      accaactga agatgagat tgcttgtca cgctcctaa 
      acccattct gattaatac tggaggcgt cgattttcc 
      atacccttg gcaacccag aattgttac aactcaaat 
      tgttactgt tggagaagt tgcgctaat tgctgttcc 
      tggagagtt taccacaat gtcaggtcg gagattaag 
      agacgcagt caaagaaac gataattag taatggtgg 
      ttccgaaaa cactcgacc aattatcac aggtcttag 
      taacgtcta tactaacta tattgcgac tccggagga 
      gtaccaatt gcaacggta cgaaggtgg ttccaccat 
      ttatggtcc acaaaccct aaacctcta cataaataa 
      attcaaaca actggccac agctttaat gaagggaga 
      aactctaga ttcaggacc aactcctcc agatttctc 
      ctcccagtt gatcacttt agtttcacc accttcagt 
      tgatggaat tgcacaagg ccaaaattt tggcgactg 
      tctccaaca accacccga atccgtttc aatcggaga 
      cactgtttc agtaaaatt tgtgagtgg caaccctcg 
      gcatgatgt tctccacgg tgggacata tattagtgt 
      ggaaaaatc ggaaggtga tgactgggt tcttgtggc 
      ttcggatgc aaactggga aacacggtt tcattggaa 
      gaggacaaa cgtattaac gggagagtc tgaggttac 
      cattgaatg ggatattgg tcaagatgt ggtaccagg 
      gcagtaccg aataagaca taatggaca ttataaaga 
      aaaatctgg agagattca cgcttatga aggcataac 
      gagtacatt taaaattgc ctgagacat caaacaatc 
      aagatttta taataaata catttaaaa acc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY