Status
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Gene information
General information
TC011137 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 4074914..4101304
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011137
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04973

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011137 RA
      MGSEHYCLR WNNHQSNLL GVFSQLLRD ESLVDVTLA 
      CSEGHSIRA HKVVLSACS SYFQTLFVD HPSRHPIVI 
      LKDVRFAEL RTLIEFMYK GEVNVEYCQ LSALLKTAE 
      SLKVKGLAE MTREYKHTE PEQTEPTEL TRRPASRSP 
      SVDENDRLH PSSQSPPVQ NLRVCRSPL EHQENDAKR 
      ASSPENTRN VSSPVTSDC GPSDMTIHS NSNNMVAAC 
      PGMNAPIIC TRLPSPITE PLPGPSNMP PVQQVPLSL 
      KKEVDWSGG LEDKNTGES SSDFSRHPH DSELEREGS 
      PRLYPCMYC GSSFPHQSK LTRHILSHS LETLKYREQ 
      LQPPPFFGG GVPPPDPTD QPPTMDVDL AEAGGGVVL 
      CKFCGKSFP DVSSLIAHL PVHTGDRPF KCEFCGKAF 
      KLRHHMKDH CRVHTGERP FRCALCGKT FSRSTILKA 
      HEKTHYPKY VRKFLSPSP LDTEEESPH
    CDS sequence
    • >TC011137 RA
      atgggaagc gagcattac tgtttgagg tggaataat 
      catcaaagc aatttactc ggggtgttc agtcaactg 
      ctccgtgac gaaagtcta gtagacgta accctggcc 
      tgctcggag ggccattcg attcgagct cacaaagtc 
      gtcctttct gcctgttcc tcctatttc cagacgctc 
      ttcgtcgac catcccagt cgtcatcct atcgtcatt 
      ctcaaagac gtccgcttt gcggagctt aggacactc 
      atcgaattc atgtacaag ggcgaagtc aacgtcgaa 
      tactgccaa ctgtcggcc ctcctcaaa acggcggag 
      agcctcaaa gtcaaggga ctcgccgaa atgaccagg 
      gaatacaaa cacacggaa ccggagcag acggagccg 
      acggagctc acccggagg ccggcgtcg cgctcgccg 
      tccgtggac gagaacgac cgcctccat ccgtcgtcg 
      cagtcgccg cccgtgcag aacctgagg gtgtgtcgg 
      tcgcccctc gagcaccag gagaacgat gcgaaaagg 
      gcgtcgtca ccggaaaac acccggaat gttagttcg 
      ccggtgaca agcgattgc ggtccgagc gacatgaca 
      attcacagt aacagcaac aacatggtg gcggcctgt 
      ccgggaatg aacgccccg attatatgc acgaggtta 
      ccctcgccg atcactgaa cctcttccg ggaccttcc 
      aacatgcca ccagttcaa caagtgcct ctgagcctc 
      aagaaggaa gttgactgg agcggaggt ctagaagac 
      aaaaataca ggagagagt tcatcagac ttttcgaga 
      cacccccac gattctgag ctggagcgc gagggctcg 
      ccccggctg tacccctgc atgtactgc ggctcttcg 
      ttcccccac cagagcaag ctgacccgg cacatcctg 
      tcgcactcg ctggagacg ctcaagtac cgcgagcag 
      ctgcagccg ccgccgttc ttcggcggc ggcgtgccg 
      ccccccgac cccaccgac cagccgccc accatggac 
      gtggacctg gcggaggcc ggcggcggc gtcgtgctg 
      tgcaagttc tgcggcaag agcttcccc gacgtgagc 
      tcgctgatc gcccatctg ccggtgcac accggcgac 
      cggcccttc aagtgcgag ttctgcggg aaggccttc 
      aagctgcgc caccacatg aaggaccac tgcagggtg 
      cacacgggc gagcggccc ttccggtgc gcgttgtgc 
      ggaaagacg ttctccagg tcgacgatc ctcaaagcg 
      cacgagaag acgcactat cccaaatat gtacgcaag 
      ttcctgtcg ccgagtcct ctcgatacc gaggaggag 
      agtccgcat tag
    mRNA sequence
    • >TC011137 RA
      cgactcgca gacgtcggc gggtgcccc acttccccg 
      atgatgcgt ctcgacgct gcactcggc gtcgcgacg 
      gccgaaggc gatgcgatg cgtatgcac cgccgtggt 
      ggggctgcg ggggcgacg gagcgcgat ccgttccga 
      cagccgcga tcagacagt tggccggcg acttgattc 
      gaagccggg tcggagcac ccagtgaga tgcacccct 
      aggagctag ggcgtcacg tgccatttt cgcgactat 
      ttggtggca gttccgggt tcggcgccc gatgctctc 
      ctcaaagac gaccacgct acatgccca agtgccact 
      tcctgctgg tgaccatta aaccagtgc tcagtgtta 
      cagttggcg ttgctgaaa ctttgtgcg ttccacgca 
      ttgcataat caatgacag aaagtgttg atcggcgtg 
      cagagagat gggaagcga gcattactg tttgaggtg 
      gaataatca tcaaagcaa tttactcgg ggtgttcag 
      tcaactgct ccgtgacga aagtctagt agacgtaac 
      cctggcctg ctcggaggg ccattcgat tcgagctca 
      caaagtcgt cctttctgc ctgttcctc ctatttcca 
      gacgctctt cgtcgacca tcccagtcg tcatcctat 
      cgtcattct caaagacgt ccgctttgc ggagcttag 
      gacactcat cgaattcat gtacaaggg cgaagtcaa 
      cgtcgaata ctgccaact gtcggccct cctcaaaac 
      ggcggagag cctcaaagt caagggact cgccgaaat 
      gaccaggga atacaaaca cacggaacc ggagcagac 
      ggagccgac ggagctcac ccggaggcc ggcgtcgcg 
      ctcgccgtc cgtggacga gaacgaccg cctccatcc 
      gtcgtcgca gtcgccgcc cgtgcagaa cctgagggt 
      gtgtcggtc gcccctcga gcaccagga gaacgatgc 
      gaaaagggc gtcgtcacc ggaaaacac ccggaatgt 
      tagttcgcc ggtgacaag cgattgcgg tccgagcga 
      catgacaat tcacagtaa cagcaacaa catggtggc 
      ggcctgtcc gggaatgaa cgccccgat tatatgcac 
      gaggttacc ctcgccgat cactgaacc tcttccggg 
      accttccaa catgccacc agttcaaca agtgcctct 
      gagcctcaa gaaggaagt tgactggag cggaggtct 
      agaagacaa aaatacagg agagagttc atcagactt 
      ttcgagaca cccccacga ttctgagct ggagcgcga 
      gggctcgcc ccggctgta cccctgcat gtactgcgg 
      ctcttcgtt cccccacca gagcaagct gacccggca 
      catcctgtc gcactcgct ggagacgct caagtaccg 
      cgagcagct gcagccgcc gccgttctt cggcggcgg 
      cgtgccgcc ccccgaccc caccgacca gccgcccac 
      catggacgt ggacctggc ggaggccgg cggcggcgt 
      cgtgctgtg caagttctg cggcaagag cttccccga 
      cgtgagctc gctgatcgc ccatctgcc ggtgcacac 
      cggcgaccg gcccttcaa gtgcgagtt ctgcgggaa 
      ggccttcaa gctgcgcca ccacatgaa ggaccactg 
      cagggtgca cacgggcga gcggccctt ccggtgcgc 
      gttgtgcgg aaagacgtt ctccaggtc gacgatcct 
      caaagcgca cgagaagac gcactatcc caaatatgt 
      acgcaagtt cctgtcgcc gagtcctct cgataccga 
      ggaggagag tccgcatta ggtgcaatc actactggc 
      ggtggcttg aacgggcta attattacg ttgttgtta 
      taagggctg cttgtatgg catctgtaa gagctgtga 
      tagaattat aacttagtt aatggtgta catttaaga 
      gttaagggt gagtccgcc tcagttttt caacgcgaa 
      cgagaggga aatgctcct gggtaccta cacgaatga 
      catggttta tttttgctt tcagttgta ataattttc 
      aattatttt ttttattct ttatttatt gttgtgcga 
      acaagagaa acagaaaac accttattt ttggtttaa 
      tgattaaaa acacacggc aattaaaaa aattatggt 
      aagaatatg gagaaggga aactgtata aaacaaaat 
      tatataaca acttttttt aatagctga tctcgtgag 
      ataagtatc ttaggatca aaagaattg agaaggaag 
      aggtagtaa gtgtgtttt ctacttgaa atgtttttt 
      ttttaatta ttgaatacg caaaaatca ttaagaaat 
      attacaata aaacaaatt aaacaaaaa aattcaaaa 
      gctgttttt ttctttaaa atataccta ttttttatt 
      gaaaagttt cacttttcc attttaaaa taaaaaaaa 
      cgttataga attcctcac cgtatttta caaattcaa 
      ttttcctat ttctttgca caaacagct atcttattg 
      tgacaaaaa aactgaatt tttaaaggt gcacacacg 
      tgactttga aggtgtaaa gttcaagaa tttataaga 
      aatcagaaa aagtgcg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY