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Gene information
General information
TC011126 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 4272566..4275537
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011126
    iBeetle screen iB_04972

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011126 RA
      METKVLKLL ELLLSETNE IKQKLNKLE ARLDNQQHI 
      DRIFGDIHR YFANNDAKT GNSPGNDSK PKQEVPANA 
      GNLEMAKCN RVTDTEPEI DNIDLKTSE ISIDDLKSV 
      LKYTLVEEI GPSHAPTFK MAVKVNEKI YYGKGGSKQ 
      KARDDVTQI VYNSISKNK VKPTIKDEK NEPRNVIID 
      LSKLKKNKL EYLKPLLKF TLIEQIGPS HAPTFKMAA 
      KVNGKLYFG NGGSKQKAQ TSVTQSVLQ SLNQKLIND 
      NLINVQIEP MTVKTDPKY VENTYSLLK YRLVEQTGP 
      DHKPTFKMA AKVNEKIYY GVGGSKVKA QMNVLELVL 
      GELLKNQKQ KQHGEVKSL SETKNVNLL PTKVQNTSK 
      SLKYVTSAL QYNFLGTTG AMFKMAAKV NGKIYTGLG 
      ISKTKARLD VATQVHTSL LTQTNECQQ PETETKAAA 
      FINRLYPNA IYTCTETRT DTRTEFTII ITVNNQQFV 
      GIGPSKKIA KKAAAFSAL TGLGDPPKT QINTVVKPE 
      FFRRLVEEK IASLMSQTP QFGRYKVGA GIVMTRNGN 
      FLDCDVVAV ATGTKCIRS AKITNSGAN LNDMHAEIL 
      TRRCLIDFF YDQLELSLE GRKETIFER NGPKFRLKD 
      GIEFHLFVD TTPCGDASV FSPRNVPRR QGFLRVKVE 
      AVGGAVSTK NERIFREEK LLTMSCSDK ICRWNVLGL 
      QGALLSNFL DPIYLKSVI LGNVIHESH LQRAIYGRV 
      KDTLVNLPS PYKLNTPST FFLPSTRQS PASVPNFAI 
      TWSKNNQQC EIINYRTGM TQLGPSKVS KQRFLQKFG 
      ALSEKIWGK NLEIMYIEA KFSAFEYNN AKNSINEAF 
      RSADLGNWI TRPIEREMF RLKN
    CDS sequence
    • >TC011126 RA
      atggaaaca aaagtgtta aaactactg gaattactg 
      ttaagtgaa actaatgaa atcaaacaa aagctcaac 
      aagttggaa gccagatta gataaccag caacacatc 
      gaccgaatc tttggtgat attcatcgt tattttgcc 
      aacaatgac gcaaaaacg ggtaattca cctggaaat 
      gattctaag cctaaacaa gaggtgcca gcgaatgct 
      ggaaatctt gaaatggct aaatgcaat agagtcaca 
      gacactgaa cctgaaatt gataatatt gacttgaag 
      acgagcgaa atatcaatt gatgattta aaatcggtg 
      ttaaaatac actttagtt gaagaaatc gggccttca 
      catgctccc acattcaaa atggcggtc aaagtgaac 
      gaaaaaatt tattatggg aaagggggc tctaaacaa 
      aaggcgcga gatgacgta acgcaaatt gtttacaat 
      tcaattagc aaaaataaa gttaaacca acaattaag 
      gatgaaaag aatgagcca cggaatgtt ataatcgac 
      ttgtcgaag ttaaaaaaa aacaaactg gaatatctc 
      aaaccttta ttaaaattc actctaatc gaacaaatc 
      gggccttca cacgcgccc acattcaaa atggcagcc 
      aaagtgaat ggaaaattg tatttcgga aatggcggc 
      tccaaacaa aaagctcaa accagtgtc actcaatcg 
      gttttacaa tcactgaat caaaaacta attaacgat 
      aatttaata aacgttcaa atcgagcca atgacagtg 
      aaaactgat ccaaaatat gtcgaaaat acatattcc 
      ttactcaaa tatcgcctt gtcgaacaa acaggaccg 
      gaccacaaa cccacattc aaaatggcc gccaaagtg 
      aatgaaaaa atttattac ggagtaggc ggttccaag 
      gtcaaggcg caaatgaac gtccttgaa ctggttctg 
      ggggaatta ctcaaaaat caaaaacaa aaacaacac 
      ggagaggtg aagtcactt tctgagacc aaaaacgtg 
      aatttgcta ccaacaaaa gtccaaaac acgtcaaaa 
      tccctaaag tacgtcaca tcggcgctg cagtacaat 
      tttctggga actaccggc gccatgttc aaaatggcg 
      gccaaggtc aacggcaaa atctacacc ggccttgga 
      atttccaaa acaaaggct agattagac gttgccaca 
      caagtccat acgtcacta ctaactcaa acaaatgaa 
      tgtcaacaa ccggaaacg gaaacaaaa gctgcggct 
      tttatcaac cgactttac cccaacgcc atctacacg 
      tgcactgag acacgaact gacactcga accgaattt 
      actatcatt atcaccgta aataatcaa caatttgta 
      ggaatcggt ccgagtaaa aaaatcgcg aaaaaagcg 
      gccgcgttt tcggccttg actggcctg ggggatccc 
      ccaaaaaca caaataaac accgtcgta aaaccggaa 
      tttttccga aggctagtc gaggagaaa atcgcttca 
      ttaatgagc caaacgcca caattcggt cgttataaa 
      gtgggcgct ggaattgtg atgacacga aatggtaac 
      tttttagat tgtgatgtg gtagcagtg gcgaccggc 
      acgaaatgc atccggagc gcgaaaatc accaacagt 
      ggcgccaac ctaaacgac atgcacgcc gaaatccta 
      acacggagg tgtttaatt gattttttc tacgaccag 
      ttggagtta agtcttgaa gggcgaaaa gaaacgatt 
      tttgagcga aatggaccg aaatttcga cttaaagac 
      ggaattgag tttcatttg tttgttgat acgaccccc 
      tgtggcgat gctagtgtt tttagtccg cggaacgtg 
      cctagaagg cagggattt ttgcgcgta aaagtcgaa 
      gcagttggt ggcgcagtt tccacgaaa aacgaacga 
      attttccgg gaagaaaaa ctattaaca atgtcttgc 
      tcggataaa atttgcaga tggaacgtc ctagggctc 
      cagggggcc cttttgtcc aatttcctc gacccgatt 
      tacctcaaa tcggtgatt ttgggtaat gtcattcac 
      gaatcacat ttacagagg gcaatttac ggacgtgtc 
      aaggacact cttgtcaat ctcccctct ccttataaa 
      ttgaatacc ccctccacg ttttttctc ccctccact 
      aggcaaagc cctgcctca gtcccaaat tttgccatc 
      acgtggtcg aaaaacaac caacagtgt gagattatc 
      aattacagg accggaatg acacaactg gggccgtct 
      aaggtttca aaacaaagg tttttgcaa aaatttgga 
      gccctcagt gagaaaatt tggggaaaa aatttggag 
      attatgtat attgaggca aaattttcg gcttttgaa 
      tataacaat gccaaaaat tcaataaat gaggctttt 
      agaagcgca gatttgggc aattggatt acaagacct 
      atagaacgc gaaatgttt agattaaaa aattaa
    mRNA sequence
    • >TC011126 RA
      tcggcgaca aagtagagt aattccccg agttattgt 
      ttcatttgt gtcttagtg tcgtgttcg ttaaacatg 
      gaaacaaaa gtgttaaaa ctactggaa ttactgtta 
      agtgaaact aatgaaatc aaacaaaag ctcaacaag 
      ttggaagcc agattagat aaccagcaa cacatcgac 
      cgaatcttt ggtgatatt catcgttat tttgccaac 
      aatgacgca aaaacgggt aattcacct ggaaatgat 
      tctaagcct aaacaagag gtgccagcg aatgctgga 
      aatcttgaa atggctaaa tgcaataga gtcacagac 
      actgaacct gaaattgat aatattgac ttgaagacg 
      agcgaaata tcaattgat gatttaaaa tcggtgtta 
      aaatacact ttagttgaa gaaatcggg ccttcacat 
      gctcccaca ttcaaaatg gcggtcaaa gtgaacgaa 
      aaaatttat tatgggaaa gggggctct aaacaaaag 
      gcgcgagat gacgtaacg caaattgtt tacaattca 
      attagcaaa aataaagtt aaaccaaca attaaggat 
      gaaaagaat gagccacgg aatgttata atcgacttg 
      tcgaagtta aaaaaaaac aaactggaa tatctcaaa 
      cctttatta aaattcact ctaatcgaa caaatcggg 
      ccttcacac gcgcccaca ttcaaaatg gcagccaaa 
      gtgaatgga aaattgtat ttcggaaat ggcggctcc 
      aaacaaaaa gctcaaacc agtgtcact caatcggtt 
      ttacaatca ctgaatcaa aaactaatt aacgataat 
      ttaataaac gttcaaatc gagccaatg acagtgaaa 
      actgatcca aaatatgtc gaaaataca tattcctta 
      ctcaaatat cgccttgtc gaacaaaca ggaccggac 
      cacaaaccc acattcaaa atggccgcc aaagtgaat 
      gaaaaaatt tattacgga gtaggcggt tccaaggtc 
      aaggcgcaa atgaacgtc cttgaactg gttctgggg 
      gaattactc aaaaatcaa aaacaaaaa caacacgga 
      gaggtgaag tcactttct gagaccaaa aacgtgaat 
      ttgctacca acaaaagtc caaaacacg tcaaaatcc 
      ctaaagtac gtcacatcg gcgctgcag tacaatttt 
      ctgggaact accggcgcc atgttcaaa atggcggcc 
      aaggtcaac ggcaaaatc tacaccggc cttggaatt 
      tccaaaaca aaggctaga ttagacgtt gccacacaa 
      gtccatacg tcactacta actcaaaca aatgaatgt 
      caacaaccg gaaacggaa acaaaagct gcggctttt 
      atcaaccga ctttacccc aacgccatc tacacgtgc 
      actgagaca cgaactgac actcgaacc gaatttact 
      atcattatc accgtaaat aatcaacaa tttgtagga 
      atcggtccg agtaaaaaa atcgcgaaa aaagcggcc 
      gcgttttcg gccttgact ggcctgggg gatccccca 
      aaaacacaa ataaacacc gtcgtaaaa ccggaattt 
      ttccgaagg ctagtcgag gagaaaatc gcttcatta 
      atgagccaa acgccacaa ttcggtcgt tataaagtg 
      ggcgctgga attgtgatg acacgaaat ggtaacttt 
      ttagattgt gatgtggta gcagtggcg accggcacg 
      aaatgcatc cggagcgcg aaaatcacc aacagtggc 
      gccaaccta aacgacatg cacgccgaa atcctaaca 
      cggaggtgt ttaattgat tttttctac gaccagttg 
      gagttaagt cttgaaggg cgaaaagaa acgattttt 
      gagcgaaat ggaccgaaa tttcgactt aaagacgga 
      attgagttt catttgttt gttgatacg accccctgt 
      ggcgatgct agtgttttt agtccgcgg aacgtgcct 
      agaaggcag ggatttttg cgcgtaaaa gtcgaagca 
      gttggtggc gcagtttcc acgaaaaac gaacgaatt 
      ttccgggaa gaaaaacta ttaacaatg tcttgctcg 
      gataaaatt tgcagatgg aacgtccta gggctccag 
      ggggccctt ttgtccaat ttcctcgac ccgatttac 
      ctcaaatcg gtgattttg ggtaatgtc attcacgaa 
      tcacattta cagagggca atttacgga cgtgtcaag 
      gacactctt gtcaatctc ccctctcct tataaattg 
      aataccccc tccacgttt tttctcccc tccactagg 
      caaagccct gcctcagtc ccaaatttt gccatcacg 
      tggtcgaaa aacaaccaa cagtgtgag attatcaat 
      tacaggacc ggaatgaca caactgggg ccgtctaag 
      gtttcaaaa caaaggttt ttgcaaaaa tttggagcc 
      ctcagtgag aaaatttgg ggaaaaaat ttggagatt 
      atgtatatt gaggcaaaa ttttcggct tttgaatat 
      aacaatgcc aaaaattca ataaatgag gcttttaga 
      agcgcagat ttgggcaat tggattaca agacctata 
      gaacgcgaa atgtttaga ttaaaaaat taattgtaa 
      ttttttatg tatttttta catttataa taaagtttt 
      ttgcaaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY