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Gene information
General information
TC011121 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450233.1: 11628..14595
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011121
    iBeetle screen iB_04971

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011121 RA
      MVNLINSTQ TTLTKIYDY YEWTLSLSD SRTKGWPLV 
      DSPGPTLLY TLIYLFIVW IGPKLMKNR KPFRLTWLL 
      IPYNLTMAV LNAYIAIQL LTASTRLRY SYICEPCRQ 
      RYHPDELQI ANAVWWYYF SKLLEFCDT FFFILRKKD 
      RQLTFLHVY HHSTMFSLW WIGIKWVPS GSTFLPAMV 
      NSAIHVLMY AYYGLAALG PHIARYLWW KKYLTILQM 
      IQFTCALIL GINGIRTGC DFPLWMHYT LIIYMLSFI 
      VLFGNFYVK AYMEKGSQV FFGMDMGCG QGNTYQRIT 
      NHHQNGKKI D
    CDS sequence
    • >TC011121 RA
      atggttaac ttaattaat tcgacccaa acgacctta 
      acgaaaatt tacgattac tacgaatgg acgttatcg 
      ctttctgat tcacgcacg aaagggtgg cccttagtc 
      gattcccct ggtccgacc ctgctctac accttgatt 
      taccttttt atagtatgg ataggtccc aagttaatg 
      aaaaaccga aaaccattt cgtttaacg tggctttta 
      atcccgtat aacttaaca atggctgtt ttaaacgcc 
      tatattgcc atacagctt ctcacagct tcgaccaga 
      ctcagatat agttacatt tgcgaaccg tgccgtcag 
      cggtaccac cctgacgag ctccagatc gcaaacgct 
      gtgtggtgg tactacttt tccaaacta ctggaattt 
      tgcgacacg tttttcttt attttgcgc aagaaagac 
      cgacaattg accttcctg catgtctac caccactcg 
      accatgttc tccctttgg tggattgga atcaaatgg 
      gttccgagt ggttcaaca tttcttcca gcaatggtc 
      aattcagcc attcatgtc ctgatgtat gcttattat 
      ggccttgcg gctttagga cctcacatt gcgaggtac 
      ttgtggtgg aagaaatat ttgactatt cttcaaatg 
      atccagttc acctgtgcc ttgattttg ggtataaac 
      ggcattcga acgggttgt gacttcccg ctgtggatg 
      cactacacc ctcatcatc tacatgttg tcctttatc 
      gtccttttc ggcaatttc tacgtgaaa gcttacatg 
      gaaaagggc agtcaggtg tttttcggc atggacatg 
      ggctgtggt caaggcaac acctaccag cgcattacc 
      aatcaccac caaaacggg aaaaagatc gactag
    mRNA sequence
    • >TC011121 RA
      gtgccagtg gggttatca atggaaagg cgcgaactt 
      gttcagttc attgtcaag tatttgagt gtttgctga 
      aattgaaaa caatacgca agaaaaatg gttaactta 
      attaattcg acccaaacg accttaacg aaaatttac 
      gattactac gaatggacg ttatcgctt tctgattca 
      cgcacgaaa gggtggccc ttagtcgat tcccctggt 
      ccgaccctg ctctacacc ttgatttac ctttttata 
      gtatggata ggtcccaag ttaatgaaa aaccgaaaa 
      ccatttcgt ttaacgtgg cttttaatc ccgtataac 
      ttaacaatg gctgtttta aacgcctat attgccata 
      cagcttctc acagcttcg accagactc agatatagt 
      tacatttgc gaaccgtgc cgtcagcgg taccaccct 
      gacgagctc cagatcgca aacgctgtg tggtggtac 
      tacttttcc aaactactg gaattttgc gacacgttt 
      ttctttatt ttgcgcaag aaagaccga caattgacc 
      ttcctgcat gtctaccac cactcgacc atgttctcc 
      ctttggtgg attggaatc aaatgggtt ccgagtggt 
      tcaacattt cttccagca atggtcaat tcagccatt 
      catgtcctg atgtatgct tattatggc cttgcggct 
      ttaggacct cacattgcg aggtacttg tggtggaag 
      aaatatttg actattctt caaatgatc cagttcacc 
      tgtgccttg attttgggt ataaacggc attcgaacg 
      ggttgtgac ttcccgctg tggatgcac tacaccctc 
      atcatctac atgttgtcc tttatcgtc cttttcggc 
      aatttctac gtgaaagct tacatggaa aagggcagt 
      caggtgttt ttcggcatg gacatgggc tgtggtcaa 
      ggcaacacc taccagcgc attaccaat caccaccaa 
      aacgggaaa aagatcgac tagacattt tttcgacaa 
      atttttaca aatcgcgat cttcctgtt caatcaaaa 
      aaaaagcaa acaaacaaa aaagtgatc tttttgatt 
      gtagatata gtgcgaaat ttttattgt tattaattt 
      tattcgtag attagtgtg gcgagtcaa ttatttagt 
      tagagagta tgataaaca ctgccattt cgcacattt 
      tttcgctca gggtctggt tttttggac cgaatcaaa 
      acactcaaa tcagtatta cgacactga tgataaacc 
      cccttgttt atgtactat ctcaaatga gacgatatg 
      ttggttgtt aaaaataaa atagagaaa agtga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY