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Gene information
General information
TC031404
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011063
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 5480493..5484939
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031404
    iBeetle screen iB_04967

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031404 RA
      MLVLFKEIS NRIKPKLGS PCIDNWVFK LHYRATTVI 
      FFVATILVT SREYIGEHI KCVSDSVNN KEFHKVIES 
      FCFFSTTFT VIRDEFNFG FGDPPHPGV FPYGLLSKP 
      PIRKHLYYQ WVPFVLFGQ GVMFMLTHF LWKSWEMGR 
      VRKLVSGLT YSSLAFLEN SVMVDGKSI PSKKEKEIT 
      IRRIKDSFF ENVKINRAW APQLILCEI LNFANVGLQ 
      AYITNKFLG GHFYTLGIK IFTQGHSIL DDVFPKVTK 
      CTFHKYGPS GTVQLHDAL CIMALNIIN EKIYIFLWF 
      WFIFLLVLS GLVLVWRFA SILLYSKSP VFGRIIFGF 
      GAKKLSFWK LKTVTRKFT YADWLFLKY LSKNLDGLV 
      FRELFGRIY EQLDDGAVF IGKEESGNK ND
    CDS sequence
    • >TC031404 RA
      atgttggtc ttgtttaaa gaaatctca aacagaatt 
      aagccaaaa ttgggctcc ccttgcatc gacaattgg 
      gttttcaaa ctgcattat cgcgccaca acagtgatt 
      ttctttgtt gcaacaatt ctagtaacg tcccgagag 
      tatattgga gaacatatc aagtgtgtc agtgattcg 
      gttaacaac aaggagttt cacaaagtg atcgaatct 
      ttttgtttc ttcagcact acgttcaca gttattcgt 
      gacgagttt aatttcggg tttggagac ccaccacat 
      cctggcgtt ttcccatat gggcttctc tccaaaccc 
      ccaattcgc aaacacctc tactaccaa tgggtgcct 
      tttgtgctg tttgggcaa ggggtgatg tttatgttg 
      acccacttc ctgtggaaa agctgggaa atgggacgg 
      gttaggaag ttagtttcc ggtttgact tattccagt 
      ctcgctttt ttggaaaat tcggtcatg gtcgacggg 
      aaatccatc ccttccaag aaagaaaag gaaattaca 
      attcggagg attaaggac tcgtttttt gaaaatgtg 
      aaaatcaac cgggcttgg gcccctcag ttaattctc 
      tgcgaaatt ttgaatttt gcaaatgtt gggcttcag 
      gcctatatc actaataag tttcttggg gggcatttt 
      tacaccctt gggatcaaa attttcact caggggcac 
      tcaatcctg gacgatgta ttcccaaaa gtgacgaag 
      tgcactttc cacaaatac ggcccttcc ggcaccgtg 
      caactccac gacgccctt tgcatcatg gccctcaac 
      ataatcaac gaaaaaatc tacattttc ctctggttt 
      tggttcatt ttcctcctt gtcctttca ggcctcgtc 
      ctggtttgg cgtttcgct tcgattttg ctctattcc 
      aaaagccca gtttttggc cgaattatt ttcggtttt 
      ggtgccaaa aagctgagt ttttggaaa ttgaaaact 
      gtgactcga aaattcact tacgcagat tggttgttt 
      ctcaagtat ttgtcgaag aatttagac ggtttagtg 
      tttagggaa ttatttggt cgcatctat gagcagttg 
      gacgatggg gccgttttt atcgggaag gaggaaagt 
      ggtaacaaa aatgattga
    mRNA sequence
    • >TC031404 RA
      tatatgtat tgaaattat ctcgtgcca gttttaaga 
      cgaagtgaa aaaaatgtt ggtcttgtt taaagaaat 
      ctcaaacag aattaagcc aaaattggg ctccccttg 
      catcgacaa ttgggtttt caaactgca ttatcgcgc 
      cacaacagt gattttctt tgttgcaac aattctagt 
      aacgtcccg agagtatat tggagaaca tatcaagtg 
      tgtcagtga ttcggttaa caacaagga gtttcacaa 
      agtgatcga atctttttg tttcttcag cactacgtt 
      cacagttat tcgtgacga gtttaattt cgggtttgg 
      agacccacc acatcctgg cgttttccc atatgggct 
      tctctccaa acccccaat tcgcaaaca cctctacta 
      ccaatgggt gccttttgt gctgtttgg gcaaggggt 
      gatgtttat gttgaccca cttcctgtg gaaaagctg 
      ggaaatggg acgggttag gaagttagt ttccggttt 
      gacttattc cagtctcgc ttttttgga aaattcggt 
      catggtcga cgggaaatc catcccttc caagaaaga 
      aaaggaaat tacaattcg gaggattaa ggactcgtt 
      ttttgaaaa tgtgaaaat caaccgggc ttgggcccc 
      tcagttaat tctctgcga aattttgaa ttttgcaaa 
      tgttgggct tcaggccta tatcactaa taagtttct 
      tggggggca tttttacac ccttgggat caaaatttt 
      cactcaggg gcactcaat cctggacga tgtattccc 
      aaaagtgac gaagtgcac tttccacaa atacggccc 
      ttccggcac cgtgcaact ccacgacgc cctttgcat 
      catggccct caacataat caacgaaaa aatctacat 
      tttcctctg gttttggtt cattttcct ccttgtcct 
      ttcaggcct cgtcctggt ttggcgttt cgcttcgat 
      tttgctcta ttccaaaag cccagtttt tggccgaat 
      tattttcgg ttttggtgc caaaaagct gagtttttg 
      gaaattgaa aactgtgac tcgaaaatt cacttacgc 
      agattggtt gtttctcaa gtatttgtc gaagaattt 
      agacggttt agtgtttag ggaattatt tggtcgcat 
      ctatgagca gttggacga tggggccgt ttttatcgg 
      gaaggagga aagtggtaa caaaaatga ttgaattgg 
      tgtaattat aggaattag ttttaatcg ctgtataaa 
      caagtaacg gtactttga aacggctga tgacggctg 
      tgtaattat ttcttcaat acaggactt tgattttca 
      aaaaaatat attatttat gcaacccag agtgaactg 
      agtcaaaat ttaataaac agtagttcg ataaatctc 
      aatcaatta ttcagcgct ggtttatag tcaataaaa 
      aacggtaac tcgttgaca ggtttctct tttccgctc 
      aattacaca cacgaatac gttccttgt cacattcta 
      attaacatt aatggaaaa ctggaaagt gcccgattt 
      tggtacaag tggagtagt aattaatta gaagttcta 
      tgttaaaga attgactcg atatttggt taaatagca 
      aatagaatt aaaatttca gtaataaaa tattgcttt 
      gcat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY