Status
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Gene information
General information
TC011056 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 5387817..5393661
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011056
    iBeetle screen iB_04966

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    1 analysable object

    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection



    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    Pupal morphology
    antenna orientation irregular - Number of animals showing the phenotype: 3

    labrum orientation irregular - Number of animals showing the phenotype: 2

    labrum size decreased - Number of animals showing the phenotype: 2
    eyes size decreased - Number of animals showing the phenotype: 3

    wings size shortened - Number of animals showing the phenotype: 2

    wings blistered - Number of animals showing the phenotype: 1

    legs orientation irregular - Number of animals showing the phenotype: 3

    legs tarsus shape irregular - Number of animals showing the phenotype: 3

    dorsal thoracic muscles partially not present - Number of animals showing the phenotype: 3

    dorsal thoracic muscles partially rounded - Number of animals showing the phenotype: 3
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011056 RA
      MSTSEKNKP SDMEEEPFG LGCALELFV EATEVSEMI 
      DTLKSIYSD KALNEKAYE RFSFIIGQY KEQPHLLDP 
      HIDHLLEKC INIVRDGGS SMDLKHEVF KYMFVFVNV 
      RGYKVIVRH LPHEVSDFE AVLRLLESQ DCDDSITWT 
      TRYVLLLWL SIIVMIPFH LSRFDANDE SEKEKKTVM 
      CRVLDIIKK YIVVPDKCR DAAAYLSSR FITRNDVKQ 
      EHLLSYIEW SMVESTSSE STIFTKQGT LASIAMILK 
      TGKRDDLLP HARPLLQWI IRDEIKHNS GTNVQKLVY 
      KIVQRIGLT FLPPKVAAW RYKRGNRSL AANLSGGDG 
      TTSSHDTQE PLPEDNIEV PDEVEEVID QLINGLRSC 
      DGIVRWSAA KGVGRVTGR LPQELADEV VGSVLELFS 
      PREGDGAWH GGCLALAEL GRRGLLLPK RLPEVVPVV 
      LKALVYDEP RGYSSVGSH IRDAACYVC WAFARAYDK 
      DVLKPFVNQ IAANLLIVA CFDREINCR RAASAAFQE 
      NVGRQGTFP YGIDILTVA DFFTVSVRN NAYLTISPY 
      IAQFGEYTI PMIDHLVGR KVDHWDCVI RELTAKALH 
      NLTPKAPEY MVTKVLPTL FDRSTSIDL NARHGSVLA 
      IGEIIHALA LTGDKSILA GHLDKIANL VPEFRQKFY 
      FRGLGGELM RTACCDFIE KCAMASLEF TNGSVTDDW 
      LNLINECIA YNVTNIQTN AIKALPTVL SRYYANQNC 
      ESLVKGYIE ELQKTSQQE VRMGHALAL GALPKFVLE 
      PQLGAIIDS LIATSQASP LTVKWAESR RDSIKALTS 
      LSVTMAEDL AKSDVLRIF DVFLASLKD YTQDKRGDV 
      GAWVREAAM LGLQTLAFK VKVSIDVAL TTQIVAGVA 
      QQAVERIDR TRALAGRIF YSFLYNDPT IPNIPHHDE 
      LLKIFEKTE CETLNWNSA SDTFPKFVE LLQFPEFSY 
      NVMLGLVCS VGGLTETLV KNSSSSLFA YISREKKCK 
      GVGEIDRLA DVIYRIFED NRKNDRIVV PMFRFLDKL 
      FSSGCIQHL ADDPSSDFC KKILKLAMN ELVGCKDAY 
      KIIDGINLL AQFVQVKSE ACQSAVVQL SIFLCHRQA 
      YVRKSTASI LYESLLVYG DSAVFNTEN LDEVMQILS 
      NTNWDDPVD VVKPARNEM CKLMGVRVP VAKTSTN
    CDS sequence
    • >TC011056 RA
      atgtcaaca agtgaaaaa aacaagcct tcagatatg 
      gaagaggag cctttcggt cttggctgc gcccttgaa 
      ttgttcgtc gaagcgacc gaagtttca gaaatgata 
      gacaccctt aaatccatt tattcggat aaagccctc 
      aatgagaag gcgtacgag cgttttagc ttcatcatt 
      ggtcagtat aaagaacag ccccacttg ctggacccc 
      catattgac caccttttg gaaaaatgt atcaatatc 
      gtacgagat gggggctcc agtatggac ttgaaacac 
      gaagttttt aagtatatg ttcgtgttt gttaacgtg 
      agggggtac aaagtgata gtcaggcat cttccacat 
      gaggtttca gattttgaa gccgtttta agactttta 
      gaatcacaa gactgtgat gattcaatc acttggacc 
      actagatat gtactattg ttgtggtta tcaataatt 
      gtcatgatt ccgtttcac ttatcacga tttgatgca 
      aatgatgaa agcgagaaa gagaagaaa acagtgatg 
      tgtagggtt ttggacatt atcaagaaa tatattgtg 
      gttcctgat aagtgcagg gacgctgcg gcttatctc 
      tcgtcgcgt tttatcaca agaaatgac gtcaaacag 
      gagcaccta ttgtcttac atcgagtgg tcaatggtt 
      gaaagtacc tcctccgag agtactatt ttcacaaaa 
      caaggaact ttagcctca atcgccatg attttgaaa 
      accggaaaa cgcgacgat ttactccct catgcccgt 
      cccctcctc caatggata atccgtgac gaaataaaa 
      cataattcc ggtaccaac gtccaaaaa cttgtctat 
      aaaatcgtc caaagaata ggtttgacg tttctacct 
      cccaaagtg gccgcttgg cgctacaaa cgcggcaat 
      cgttcattg gctgcgaat ttaagtggg ggtgatggt 
      accacctcc tctcacgac acgcaagaa cccctccca 
      gaagataat attgaggtt cctgatgaa gtcgaggag 
      gttatagac cagttaatt aacggtttg aggagttgt 
      gatgggatt gtgcgatgg tcggctgca aagggagta 
      ggccgggta accggtcgg ttacctcaa gaactggcc 
      gatgaagtg gtcggttcg gttttggag ttgtttagt 
      ccgagagag ggggatggg gcgtggcat ggcggctgt 
      ttggcattg gctgaattg gggaggagg ggcttgttg 
      ctaccgaaa cggttaccg gaggtggta ccggttgta 
      cttaaggcg ttggtctat gatgaaccg agaggatat 
      tcctctgta ggatcacat ataagggat gcagcgtgt 
      tatgtgtgt tgggccttt gcaagggcc tatgataag 
      gatgtttta aaacctttt gttaatcag atagctgcg 
      aatttgttg atcgtcgct tgtttcgac agagagatc 
      aattgtcga cgagcggcc tctgccgcc tttcaagaa 
      aacgtcgga cgtcaagga acattcccg tacggaatt 
      gacattttg acagttgct gactttttc actgtcagt 
      gtgcgaaac aacgcctac ttgaccata agtccgtac 
      atcgcccaa ttcggcgaa tacaccatc ccaatgatc 
      gaccatcta gttggccga aaagtcgac cactgggat 
      tgcgtcatt cgggaattg acagccaaa gctttgcat 
      aatttgaca ccaaaagcg cctgaatat atggtaaca 
      aaagtgtta ccaaccttg ttcgacaga agcactagc 
      attgatttg aatgcccgc catggctcc gtattggcc 
      atcggcgaa atcatccat gccttggct ttaactggc 
      gacaagtcg atcctagcc ggccatctt gataaaatc 
      gcaaacttg gttccggaa tttcggcaa aaattctac 
      ttccgaggc ttaggaggc gaattaatg cgaacggcg 
      tgttgcgat tttatcgag aagtgtgcc atggcgtcg 
      ctcgaattc accaatggg agtgtgaca gacgattgg 
      ctcaacttg atcaacgaa tgtatcgca tacaatgtc 
      accaacatc caaacaaac gcaattaag gcgctacca 
      actgtcttg agtcgttat tacgccaat caaaattgc 
      gaaagtttg gttaaagga tatatcgaa gagttgcaa 
      aaaacgagt caacaggag gtccgaatg gggcacgcc 
      ttggctttg ggggccctc ccgaaattt gtgttggag 
      ccccaattg ggggctatc attgactcg ctaattgcc 
      acgagtcaa gcgagtcca ttgacggtc aaatgggca 
      gaaagtcgc cgagattct atcaaggct ttgacctca 
      ttgagtgtg acaatggcc gaagatttg gcaaagtcg 
      gatgttttg cgaattttt gacgttttt ttggccagt 
      ttgaaggat tatacgcag gataagaga ggcgatgtg 
      ggcgcatgg gttcgcgag gcggccatg ttgggcctc 
      cagactctg gcttttaag gtcaaagtg tcaatcgac 
      gtagccttg actacgcag atcgtagcc ggggtggcg 
      cagcaggct gtggagcgc atcgaccgg acgcgcgca 
      ttggctggt cgaattttt tacagtttc ctgtacaac 
      gaccccacc atcccgaat atacctcac cacgacgaa 
      ttgttgaaa atttttgaa aaaaccgag tgtgagacg 
      ttgaactgg aattcggcg agtgatacg ttcccgaaa 
      tttgtcgaa ttgttgcaa tttcccgaa tttagctac 
      aatgtgatg ttggggttg gtttgcagt gtgggcggt 
      ttgaccgaa actctggtc aaaaattcg agttcgtcg 
      ctctttgcc tacatctca agagagaaa aagtgtaaa 
      ggggtgggg gagattgat aggttggcc gacgttatc 
      tataggatt tttgaggat aataggaaa aatgaccga 
      attgtggtc ccgatgttc cgttttttg gataaactt 
      ttcagctcg gggtgtata caacatttg gcggatgac 
      ccgagtagc gatttttgt aaaaaaatc ctcaagttg 
      gccatgaat gaattggtg gggtgcaaa gacgcgtat 
      aaaattata gatggtatt aatcttttg gcgcagttt 
      gttcaggtc aaaagtgag gcctgtcag tcggctgta 
      gtccagttg agtattttt ttgtgtcat cgacaggct 
      tatgtgagg aaatcgaca gcgtctatt ttatacgaa 
      tcgctacta gtgtatgga gattctgct gtttttaat 
      actgaaaat ttggacgaa gttatgcaa attttgagt 
      aacacaaac tgggatgat ccggttgat gtggtcaaa 
      cctgcgagg aatgaaatg tgtaaatta atgggggtc 
      cgagtgcct gttgccaaa acttccact aactaa
    mRNA sequence
    • >TC011056 RA
      taaaacagt agtcgaaac ttaacctca caatttcta 
      atttttcgt gacatctgt aaacaaaag accttttta 
      tcgcaaaaa catgtcaac aagtgaaaa aaacaagcc 
      ttcagatat ggaagagga gcctttcgg tcttggctg 
      cgcccttga attgttcgt cgaagcgac cgaagtttc 
      agaaatgat agacaccct taaatccat ttattcgga 
      taaagccct caatgagaa ggcgtacga gcgttttag 
      cttcatcat tggtcagta taaagaaca gccccactt 
      gctggaccc ccatattga ccacctttt ggaaaaatg 
      tatcaatat cgtacgaga tgggggctc cagtatgga 
      cttgaaaca cgaagtttt taagtatat gttcgtgtt 
      tgttaacgt gagggggta caaagtgat agtcaggca 
      tcttccaca tgaggtttc agattttga agccgtttt 
      aagactttt agaatcaca agactgtga tgattcaat 
      cacttggac cactagata tgtactatt gttgtggtt 
      atcaataat tgtcatgat tccgtttca cttatcacg 
      atttgatgc aaatgatga aagcgagaa agagaagaa 
      aacagtgat gtgtagggt tttggacat tatcaagaa 
      atatattgt ggttcctga taagtgcag ggacgctgc 
      ggcttatct ctcgtcgcg ttttatcac aagaaatga 
      cgtcaaaca ggagcacct attgtctta catcgagtg 
      gtcaatggt tgaaagtac ctcctccga gagtactat 
      tttcacaaa acaaggaac tttagcctc aatcgccat 
      gattttgaa aaccggaaa acgcgacga tttactccc 
      tcatgcccg tcccctcct ccaatggat aatccgtga 
      cgaaataaa acataattc cggtaccaa cgtccaaaa 
      acttgtcta taaaatcgt ccaaagaat aggtttgac 
      gtttctacc tcccaaagt ggccgcttg gcgctacaa 
      acgcggcaa tcgttcatt ggctgcgaa tttaagtgg 
      gggtgatgg taccacctc ctctcacga cacgcaaga 
      acccctccc agaagataa tattgaggt tcctgatga 
      agtcgagga ggttataga ccagttaat taacggttt 
      gaggagttg tgatgggat tgtgcgatg gtcggctgc 
      aaagggagt aggccgggt aaccggtcg gttacctca 
      agaactggc cgatgaagt ggtcggttc ggttttgga 
      gttgtttag tccgagaga gggggatgg ggcgtggca 
      tggcggctg tttggcatt ggctgaatt ggggaggag 
      gggcttgtt gctaccgaa acggttacc ggaggtggt 
      accggttgt acttaaggc gttggtcta tgatgaacc 
      gagaggata ttcctctgt aggatcaca tataaggga 
      tgcagcgtg ttatgtgtg ttgggcctt tgcaagggc 
      ctatgataa ggatgtttt aaaaccttt tgttaatca 
      gatagctgc gaatttgtt gatcgtcgc ttgtttcga 
      cagagagat caattgtcg acgagcggc ctctgccgc 
      ctttcaaga aaacgtcgg acgtcaagg aacattccc 
      gtacggaat tgacatttt gacagttgc tgacttttt 
      cactgtcag tgtgcgaaa caacgccta cttgaccat 
      aagtccgta catcgccca attcggcga atacaccat 
      cccaatgat cgaccatct agttggccg aaaagtcga 
      ccactggga ttgcgtcat tcgggaatt gacagccaa 
      agctttgca taatttgac accaaaagc gcctgaata 
      tatggtaac aaaagtgtt accaacctt gttcgacag 
      aagcactag cattgattt gaatgcccg ccatggctc 
      cgtattggc catcggcga aatcatcca tgccttggc 
      tttaactgg cgacaagtc gatcctagc cggccatct 
      tgataaaat cgcaaactt ggttccgga atttcggca 
      aaaattcta cttccgagg cttaggagg cgaattaat 
      gcgaacggc gtgttgcga ttttatcga gaagtgtgc 
      catggcgtc gctcgaatt caccaatgg gagtgtgac 
      agacgattg gctcaactt gatcaacga atgtatcgc 
      atacaatgt caccaacat ccaaacaaa cgcaattaa 
      ggcgctacc aactgtctt gagtcgtta ttacgccaa 
      tcaaaattg cgaaagttt ggttaaagg atatatcga 
      agagttgca aaaaacgag tcaacagga ggtccgaat 
      ggggcacgc cttggcttt gggggccct cccgaaatt 
      tgtgttgga gccccaatt gggggctat cattgactc 
      gctaattgc cacgagtca agcgagtcc attgacggt 
      caaatgggc agaaagtcg ccgagattc tatcaaggc 
      tttgacctc attgagtgt gacaatggc cgaagattt 
      ggcaaagtc ggatgtttt gcgaatttt tgacgtttt 
      tttggccag tttgaagga ttatacgca ggataagag 
      aggcgatgt gggcgcatg ggttcgcga ggcggccat 
      gttgggcct ccagactct ggcttttaa ggtcaaagt 
      gtcaatcga cgtagcctt gactacgca gatcgtagc 
      cggggtggc gcagcaggc tgtggagcg catcgaccg 
      gacgcgcgc attggctgg tcgaatttt ttacagttt 
      cctgtacaa cgaccccac catcccgaa tatacctca 
      ccacgacga attgttgaa aatttttga aaaaaccga 
      gtgtgagac gttgaactg gaattcggc gagtgatac 
      gttcccgaa atttgtcga attgttgca atttcccga 
      atttagcta caatgtgat gttggggtt ggtttgcag 
      tgtgggcgg tttgaccga aactctggt caaaaattc 
      gagttcgtc gctctttgc ctacatctc aagagagaa 
      aaagtgtaa aggggtggg ggagattga taggttggc 
      cgacgttat ctataggat ttttgagga taataggaa 
      aaatgaccg aattgtggt cccgatgtt ccgtttttt 
      ggataaact tttcagctc ggggtgtat acaacattt 
      ggcggatga cccgagtag cgatttttg taaaaaaat 
      cctcaagtt ggccatgaa tgaattggt ggggtgcaa 
      agacgcgta taaaattat agatggtat taatctttt 
      ggcgcagtt tgttcaggt caaaagtga ggcctgtca 
      gtcggctgt agtccagtt gagtatttt tttgtgtca 
      tcgacaggc ttatgtgag gaaatcgac agcgtctat 
      tttatacga atcgctact agtgtatgg agattctgc 
      tgtttttaa tactgaaaa tttggacga agttatgca 
      aattttgag taacacaaa ctgggatga tccggttga 
      tgtggtcaa acctgcgag gaatgaaat gtgtaaatt 
      aatgggggt ccgagtgcc tgttgccaa aacttccac 
      taactaaat gatcagtgc tttttatat ttattaatt 
      tttactatt gtgttacta aataaataa tacggaaag 
      ta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY