Status
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Gene information
General information
TC011056 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 5387817..5393661
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011056
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04966

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    1 analysable object
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection

    Pupal morphology
    antenna orientation irregular - Number of animals showing the phenotype: 3

    labrum orientation irregular - Number of animals showing the phenotype: 2

    labrum size decreased - Number of animals showing the phenotype: 2
    eyes size decreased - Number of animals showing the phenotype: 3

    wings size shortened - Number of animals showing the phenotype: 2

    wings blistered - Number of animals showing the phenotype: 1

    legs orientation irregular - Number of animals showing the phenotype: 3

    legs tarsus shape irregular - Number of animals showing the phenotype: 3

    dorsal thoracic muscles partially not present - Number of animals showing the phenotype: 3

    dorsal thoracic muscles partially rounded - Number of animals showing the phenotype: 3
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011056 RA
      MSTSEKNKP SDMEEEPFG LGCALELFV EATEVSEMI 
DTLKSIYSD KALNEKAYE RFSFIIGQY KEQPHLLDP 
HIDHLLEKC INIVRDGGS SMDLKHEVF KYMFVFVNV 
RGYKVIVRH LPHEVSDFE AVLRLLESQ DCDDSITWT 
TRYVLLLWL SIIVMIPFH LSRFDANDE SEKEKKTVM 
CRVLDIIKK YIVVPDKCR DAAAYLSSR FITRNDVKQ 
EHLLSYIEW SMVESTSSE STIFTKQGT LASIAMILK 
TGKRDDLLP HARPLLQWI IRDEIKHNS GTNVQKLVY 
KIVQRIGLT FLPPKVAAW RYKRGNRSL AANLSGGDG 
TTSSHDTQE PLPEDNIEV PDEVEEVID QLINGLRSC 
DGIVRWSAA KGVGRVTGR LPQELADEV VGSVLELFS 
PREGDGAWH GGCLALAEL GRRGLLLPK RLPEVVPVV 
LKALVYDEP RGYSSVGSH IRDAACYVC WAFARAYDK 
DVLKPFVNQ IAANLLIVA CFDREINCR RAASAAFQE 
NVGRQGTFP YGIDILTVA DFFTVSVRN NAYLTISPY 
IAQFGEYTI PMIDHLVGR KVDHWDCVI RELTAKALH 
NLTPKAPEY MVTKVLPTL FDRSTSIDL NARHGSVLA 
IGEIIHALA LTGDKSILA GHLDKIANL VPEFRQKFY 
FRGLGGELM RTACCDFIE KCAMASLEF TNGSVTDDW 
LNLINECIA YNVTNIQTN AIKALPTVL SRYYANQNC 
ESLVKGYIE ELQKTSQQE VRMGHALAL GALPKFVLE 
PQLGAIIDS LIATSQASP LTVKWAESR RDSIKALTS 
LSVTMAEDL AKSDVLRIF DVFLASLKD YTQDKRGDV 
GAWVREAAM LGLQTLAFK VKVSIDVAL TTQIVAGVA 
QQAVERIDR TRALAGRIF YSFLYNDPT IPNIPHHDE 
LLKIFEKTE CETLNWNSA SDTFPKFVE LLQFPEFSY 
NVMLGLVCS VGGLTETLV KNSSSSLFA YISREKKCK 
GVGEIDRLA DVIYRIFED NRKNDRIVV PMFRFLDKL 
FSSGCIQHL ADDPSSDFC KKILKLAMN ELVGCKDAY 
KIIDGINLL AQFVQVKSE ACQSAVVQL SIFLCHRQA 
YVRKSTASI LYESLLVYG DSAVFNTEN LDEVMQILS 
NTNWDDPVD VVKPARNEM CKLMGVRVP VAKTSTN
    CDS sequence
    • >TC011056 RA
      atgtcaaca agtgaaaaa aacaagcct tcagatatg 
gaagaggag cctttcggt cttggctgc gcccttgaa 
ttgttcgtc gaagcgacc gaagtttca gaaatgata 
gacaccctt aaatccatt tattcggat aaagccctc 
aatgagaag gcgtacgag cgttttagc ttcatcatt 
ggtcagtat aaagaacag ccccacttg ctggacccc 
catattgac caccttttg gaaaaatgt atcaatatc 
gtacgagat gggggctcc agtatggac ttgaaacac 
gaagttttt aagtatatg ttcgtgttt gttaacgtg 
agggggtac aaagtgata gtcaggcat cttccacat 
gaggtttca gattttgaa gccgtttta agactttta 
gaatcacaa gactgtgat gattcaatc acttggacc 
actagatat gtactattg ttgtggtta tcaataatt 
gtcatgatt ccgtttcac ttatcacga tttgatgca 
aatgatgaa agcgagaaa gagaagaaa acagtgatg 
tgtagggtt ttggacatt atcaagaaa tatattgtg 
gttcctgat aagtgcagg gacgctgcg gcttatctc 
tcgtcgcgt tttatcaca agaaatgac gtcaaacag 
gagcaccta ttgtcttac atcgagtgg tcaatggtt 
gaaagtacc tcctccgag agtactatt ttcacaaaa 
caaggaact ttagcctca atcgccatg attttgaaa 
accggaaaa cgcgacgat ttactccct catgcccgt 
cccctcctc caatggata atccgtgac gaaataaaa 
cataattcc ggtaccaac gtccaaaaa cttgtctat 
aaaatcgtc caaagaata ggtttgacg tttctacct 
cccaaagtg gccgcttgg cgctacaaa cgcggcaat 
cgttcattg gctgcgaat ttaagtggg ggtgatggt 
accacctcc tctcacgac acgcaagaa cccctccca 
gaagataat attgaggtt cctgatgaa gtcgaggag 
gttatagac cagttaatt aacggtttg aggagttgt 
gatgggatt gtgcgatgg tcggctgca aagggagta 
ggccgggta accggtcgg ttacctcaa gaactggcc 
gatgaagtg gtcggttcg gttttggag ttgtttagt 
ccgagagag ggggatggg gcgtggcat ggcggctgt 
ttggcattg gctgaattg gggaggagg ggcttgttg 
ctaccgaaa cggttaccg gaggtggta ccggttgta 
cttaaggcg ttggtctat gatgaaccg agaggatat 
tcctctgta ggatcacat ataagggat gcagcgtgt 
tatgtgtgt tgggccttt gcaagggcc tatgataag 
gatgtttta aaacctttt gttaatcag atagctgcg 
aatttgttg atcgtcgct tgtttcgac agagagatc 
aattgtcga cgagcggcc tctgccgcc tttcaagaa 
aacgtcgga cgtcaagga acattcccg tacggaatt 
gacattttg acagttgct gactttttc actgtcagt 
gtgcgaaac aacgcctac ttgaccata agtccgtac 
atcgcccaa ttcggcgaa tacaccatc ccaatgatc 
gaccatcta gttggccga aaagtcgac cactgggat 
tgcgtcatt cgggaattg acagccaaa gctttgcat 
aatttgaca ccaaaagcg cctgaatat atggtaaca 
aaagtgtta ccaaccttg ttcgacaga agcactagc 
attgatttg aatgcccgc catggctcc gtattggcc 
atcggcgaa atcatccat gccttggct ttaactggc 
gacaagtcg atcctagcc ggccatctt gataaaatc 
gcaaacttg gttccggaa tttcggcaa aaattctac 
ttccgaggc ttaggaggc gaattaatg cgaacggcg 
tgttgcgat tttatcgag aagtgtgcc atggcgtcg 
ctcgaattc accaatggg agtgtgaca gacgattgg 
ctcaacttg atcaacgaa tgtatcgca tacaatgtc 
accaacatc caaacaaac gcaattaag gcgctacca 
actgtcttg agtcgttat tacgccaat caaaattgc 
gaaagtttg gttaaagga tatatcgaa gagttgcaa 
aaaacgagt caacaggag gtccgaatg gggcacgcc 
ttggctttg ggggccctc ccgaaattt gtgttggag 
ccccaattg ggggctatc attgactcg ctaattgcc 
acgagtcaa gcgagtcca ttgacggtc aaatgggca 
gaaagtcgc cgagattct atcaaggct ttgacctca 
ttgagtgtg acaatggcc gaagatttg gcaaagtcg 
gatgttttg cgaattttt gacgttttt ttggccagt 
ttgaaggat tatacgcag gataagaga ggcgatgtg 
ggcgcatgg gttcgcgag gcggccatg ttgggcctc 
cagactctg gcttttaag gtcaaagtg tcaatcgac 
gtagccttg actacgcag atcgtagcc ggggtggcg 
cagcaggct gtggagcgc atcgaccgg acgcgcgca 
ttggctggt cgaattttt tacagtttc ctgtacaac 
gaccccacc atcccgaat atacctcac cacgacgaa 
ttgttgaaa atttttgaa aaaaccgag tgtgagacg 
ttgaactgg aattcggcg agtgatacg ttcccgaaa 
tttgtcgaa ttgttgcaa tttcccgaa tttagctac 
aatgtgatg ttggggttg gtttgcagt gtgggcggt 
ttgaccgaa actctggtc aaaaattcg agttcgtcg 
ctctttgcc tacatctca agagagaaa aagtgtaaa 
ggggtgggg gagattgat aggttggcc gacgttatc 
tataggatt tttgaggat aataggaaa aatgaccga 
attgtggtc ccgatgttc cgttttttg gataaactt 
ttcagctcg gggtgtata caacatttg gcggatgac 
ccgagtagc gatttttgt aaaaaaatc ctcaagttg 
gccatgaat gaattggtg gggtgcaaa gacgcgtat 
aaaattata gatggtatt aatcttttg gcgcagttt 
gttcaggtc aaaagtgag gcctgtcag tcggctgta 
gtccagttg agtattttt ttgtgtcat cgacaggct 
tatgtgagg aaatcgaca gcgtctatt ttatacgaa 
tcgctacta gtgtatgga gattctgct gtttttaat 
actgaaaat ttggacgaa gttatgcaa attttgagt 
aacacaaac tgggatgat ccggttgat gtggtcaaa 
cctgcgagg aatgaaatg tgtaaatta atgggggtc 
cgagtgcct gttgccaaa acttccact aactaa
    mRNA sequence
    • >TC011056 RA
      taaaacagt agtcgaaac ttaacctca caatttcta 
atttttcgt gacatctgt aaacaaaag accttttta 
tcgcaaaaa catgtcaac aagtgaaaa aaacaagcc 
ttcagatat ggaagagga gcctttcgg tcttggctg 
cgcccttga attgttcgt cgaagcgac cgaagtttc 
agaaatgat agacaccct taaatccat ttattcgga 
taaagccct caatgagaa ggcgtacga gcgttttag 
cttcatcat tggtcagta taaagaaca gccccactt 
gctggaccc ccatattga ccacctttt ggaaaaatg 
tatcaatat cgtacgaga tgggggctc cagtatgga 
cttgaaaca cgaagtttt taagtatat gttcgtgtt 
tgttaacgt gagggggta caaagtgat agtcaggca 
tcttccaca tgaggtttc agattttga agccgtttt 
aagactttt agaatcaca agactgtga tgattcaat 
cacttggac cactagata tgtactatt gttgtggtt 
atcaataat tgtcatgat tccgtttca cttatcacg 
atttgatgc aaatgatga aagcgagaa agagaagaa 
aacagtgat gtgtagggt tttggacat tatcaagaa 
atatattgt ggttcctga taagtgcag ggacgctgc 
ggcttatct ctcgtcgcg ttttatcac aagaaatga 
cgtcaaaca ggagcacct attgtctta catcgagtg 
gtcaatggt tgaaagtac ctcctccga gagtactat 
tttcacaaa acaaggaac tttagcctc aatcgccat 
gattttgaa aaccggaaa acgcgacga tttactccc 
tcatgcccg tcccctcct ccaatggat aatccgtga 
cgaaataaa acataattc cggtaccaa cgtccaaaa 
acttgtcta taaaatcgt ccaaagaat aggtttgac 
gtttctacc tcccaaagt ggccgcttg gcgctacaa 
acgcggcaa tcgttcatt ggctgcgaa tttaagtgg 
gggtgatgg taccacctc ctctcacga cacgcaaga 
acccctccc agaagataa tattgaggt tcctgatga 
agtcgagga ggttataga ccagttaat taacggttt 
gaggagttg tgatgggat tgtgcgatg gtcggctgc 
aaagggagt aggccgggt aaccggtcg gttacctca 
agaactggc cgatgaagt ggtcggttc ggttttgga 
gttgtttag tccgagaga gggggatgg ggcgtggca 
tggcggctg tttggcatt ggctgaatt ggggaggag 
gggcttgtt gctaccgaa acggttacc ggaggtggt 
accggttgt acttaaggc gttggtcta tgatgaacc 
gagaggata ttcctctgt aggatcaca tataaggga 
tgcagcgtg ttatgtgtg ttgggcctt tgcaagggc 
ctatgataa ggatgtttt aaaaccttt tgttaatca 
gatagctgc gaatttgtt gatcgtcgc ttgtttcga 
cagagagat caattgtcg acgagcggc ctctgccgc 
ctttcaaga aaacgtcgg acgtcaagg aacattccc 
gtacggaat tgacatttt gacagttgc tgacttttt 
cactgtcag tgtgcgaaa caacgccta cttgaccat 
aagtccgta catcgccca attcggcga atacaccat 
cccaatgat cgaccatct agttggccg aaaagtcga 
ccactggga ttgcgtcat tcgggaatt gacagccaa 
agctttgca taatttgac accaaaagc gcctgaata 
tatggtaac aaaagtgtt accaacctt gttcgacag 
aagcactag cattgattt gaatgcccg ccatggctc 
cgtattggc catcggcga aatcatcca tgccttggc 
tttaactgg cgacaagtc gatcctagc cggccatct 
tgataaaat cgcaaactt ggttccgga atttcggca 
aaaattcta cttccgagg cttaggagg cgaattaat 
gcgaacggc gtgttgcga ttttatcga gaagtgtgc 
catggcgtc gctcgaatt caccaatgg gagtgtgac 
agacgattg gctcaactt gatcaacga atgtatcgc 
atacaatgt caccaacat ccaaacaaa cgcaattaa 
ggcgctacc aactgtctt gagtcgtta ttacgccaa 
tcaaaattg cgaaagttt ggttaaagg atatatcga 
agagttgca aaaaacgag tcaacagga ggtccgaat 
ggggcacgc cttggcttt gggggccct cccgaaatt 
tgtgttgga gccccaatt gggggctat cattgactc 
gctaattgc cacgagtca agcgagtcc attgacggt 
caaatgggc agaaagtcg ccgagattc tatcaaggc 
tttgacctc attgagtgt gacaatggc cgaagattt 
ggcaaagtc ggatgtttt gcgaatttt tgacgtttt 
tttggccag tttgaagga ttatacgca ggataagag 
aggcgatgt gggcgcatg ggttcgcga ggcggccat 
gttgggcct ccagactct ggcttttaa ggtcaaagt 
gtcaatcga cgtagcctt gactacgca gatcgtagc 
cggggtggc gcagcaggc tgtggagcg catcgaccg 
gacgcgcgc attggctgg tcgaatttt ttacagttt 
cctgtacaa cgaccccac catcccgaa tatacctca 
ccacgacga attgttgaa aatttttga aaaaaccga 
gtgtgagac gttgaactg gaattcggc gagtgatac 
gttcccgaa atttgtcga attgttgca atttcccga 
atttagcta caatgtgat gttggggtt ggtttgcag 
tgtgggcgg tttgaccga aactctggt caaaaattc 
gagttcgtc gctctttgc ctacatctc aagagagaa 
aaagtgtaa aggggtggg ggagattga taggttggc 
cgacgttat ctataggat ttttgagga taataggaa 
aaatgaccg aattgtggt cccgatgtt ccgtttttt 
ggataaact tttcagctc ggggtgtat acaacattt 
ggcggatga cccgagtag cgatttttg taaaaaaat 
cctcaagtt ggccatgaa tgaattggt ggggtgcaa 
agacgcgta taaaattat agatggtat taatctttt 
ggcgcagtt tgttcaggt caaaagtga ggcctgtca 
gtcggctgt agtccagtt gagtatttt tttgtgtca 
tcgacaggc ttatgtgag gaaatcgac agcgtctat 
tttatacga atcgctact agtgtatgg agattctgc 
tgtttttaa tactgaaaa tttggacga agttatgca 
aattttgag taacacaaa ctgggatga tccggttga 
tgtggtcaa acctgcgag gaatgaaat gtgtaaatt 
aatgggggt ccgagtgcc tgttgccaa aacttccac 
taactaaat gatcagtgc tttttatat ttattaatt 
tttactatt gtgttacta aataaataa tacggaaag 
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