General information | |||
TC011056 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007425.3: 5387817..5393661 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC011056 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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Quotation | ||
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Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_04966 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
1 analysable object |
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 5 (11 dpi)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
labrum orientation irregular - Number of animals showing the phenotype: 2
labrum size decreased - Number of animals showing the phenotype: 2
eyes size decreased - Number of animals showing the phenotype: 3
wings size shortened - Number of animals showing the phenotype: 2
wings blistered - Number of animals showing the phenotype: 1
legs orientation irregular - Number of animals showing the phenotype: 3
legs tarsus shape irregular - Number of animals showing the phenotype: 3
dorsal thoracic muscles partially not present - Number of animals showing the phenotype: 3
dorsal thoracic muscles partially rounded - Number of animals showing the phenotype: 3
- >TC011056 RA
MSTSEKNKP SDMEEEPFG LGCALELFV EATEVSEMI DTLKSIYSD KALNEKAYE RFSFIIGQY KEQPHLLDP HIDHLLEKC INIVRDGGS SMDLKHEVF KYMFVFVNV RGYKVIVRH LPHEVSDFE AVLRLLESQ DCDDSITWT TRYVLLLWL SIIVMIPFH LSRFDANDE SEKEKKTVM CRVLDIIKK YIVVPDKCR DAAAYLSSR FITRNDVKQ EHLLSYIEW SMVESTSSE STIFTKQGT LASIAMILK TGKRDDLLP HARPLLQWI IRDEIKHNS GTNVQKLVY KIVQRIGLT FLPPKVAAW RYKRGNRSL AANLSGGDG TTSSHDTQE PLPEDNIEV PDEVEEVID QLINGLRSC DGIVRWSAA KGVGRVTGR LPQELADEV VGSVLELFS PREGDGAWH GGCLALAEL GRRGLLLPK RLPEVVPVV LKALVYDEP RGYSSVGSH IRDAACYVC WAFARAYDK DVLKPFVNQ IAANLLIVA CFDREINCR RAASAAFQE NVGRQGTFP YGIDILTVA DFFTVSVRN NAYLTISPY IAQFGEYTI PMIDHLVGR KVDHWDCVI RELTAKALH NLTPKAPEY MVTKVLPTL FDRSTSIDL NARHGSVLA IGEIIHALA LTGDKSILA GHLDKIANL VPEFRQKFY FRGLGGELM RTACCDFIE KCAMASLEF TNGSVTDDW LNLINECIA YNVTNIQTN AIKALPTVL SRYYANQNC ESLVKGYIE ELQKTSQQE VRMGHALAL GALPKFVLE PQLGAIIDS LIATSQASP LTVKWAESR RDSIKALTS LSVTMAEDL AKSDVLRIF DVFLASLKD YTQDKRGDV GAWVREAAM LGLQTLAFK VKVSIDVAL TTQIVAGVA QQAVERIDR TRALAGRIF YSFLYNDPT IPNIPHHDE LLKIFEKTE CETLNWNSA SDTFPKFVE LLQFPEFSY NVMLGLVCS VGGLTETLV KNSSSSLFA YISREKKCK GVGEIDRLA DVIYRIFED NRKNDRIVV PMFRFLDKL FSSGCIQHL ADDPSSDFC KKILKLAMN ELVGCKDAY KIIDGINLL AQFVQVKSE ACQSAVVQL SIFLCHRQA YVRKSTASI LYESLLVYG DSAVFNTEN LDEVMQILS NTNWDDPVD VVKPARNEM CKLMGVRVP VAKTSTN
- >TC011056 RA
atgtcaaca agtgaaaaa aacaagcct tcagatatg gaagaggag cctttcggt cttggctgc gcccttgaa ttgttcgtc gaagcgacc gaagtttca gaaatgata gacaccctt aaatccatt tattcggat aaagccctc aatgagaag gcgtacgag cgttttagc ttcatcatt ggtcagtat aaagaacag ccccacttg ctggacccc catattgac caccttttg gaaaaatgt atcaatatc gtacgagat gggggctcc agtatggac ttgaaacac gaagttttt aagtatatg ttcgtgttt gttaacgtg agggggtac aaagtgata gtcaggcat cttccacat gaggtttca gattttgaa gccgtttta agactttta gaatcacaa gactgtgat gattcaatc acttggacc actagatat gtactattg ttgtggtta tcaataatt gtcatgatt ccgtttcac ttatcacga tttgatgca aatgatgaa agcgagaaa gagaagaaa acagtgatg tgtagggtt ttggacatt atcaagaaa tatattgtg gttcctgat aagtgcagg gacgctgcg gcttatctc tcgtcgcgt tttatcaca agaaatgac gtcaaacag gagcaccta ttgtcttac atcgagtgg tcaatggtt gaaagtacc tcctccgag agtactatt ttcacaaaa caaggaact ttagcctca atcgccatg attttgaaa accggaaaa cgcgacgat ttactccct catgcccgt cccctcctc caatggata atccgtgac gaaataaaa cataattcc ggtaccaac gtccaaaaa cttgtctat aaaatcgtc caaagaata ggtttgacg tttctacct cccaaagtg gccgcttgg cgctacaaa cgcggcaat cgttcattg gctgcgaat ttaagtggg ggtgatggt accacctcc tctcacgac acgcaagaa cccctccca gaagataat attgaggtt cctgatgaa gtcgaggag gttatagac cagttaatt aacggtttg aggagttgt gatgggatt gtgcgatgg tcggctgca aagggagta ggccgggta accggtcgg ttacctcaa gaactggcc gatgaagtg gtcggttcg gttttggag ttgtttagt ccgagagag ggggatggg gcgtggcat ggcggctgt ttggcattg gctgaattg gggaggagg ggcttgttg ctaccgaaa cggttaccg gaggtggta ccggttgta cttaaggcg ttggtctat gatgaaccg agaggatat tcctctgta ggatcacat ataagggat gcagcgtgt tatgtgtgt tgggccttt gcaagggcc tatgataag gatgtttta aaacctttt gttaatcag atagctgcg aatttgttg atcgtcgct tgtttcgac agagagatc aattgtcga cgagcggcc tctgccgcc tttcaagaa aacgtcgga cgtcaagga acattcccg tacggaatt gacattttg acagttgct gactttttc actgtcagt gtgcgaaac aacgcctac ttgaccata agtccgtac atcgcccaa ttcggcgaa tacaccatc ccaatgatc gaccatcta gttggccga aaagtcgac cactgggat tgcgtcatt cgggaattg acagccaaa gctttgcat aatttgaca ccaaaagcg cctgaatat atggtaaca aaagtgtta ccaaccttg ttcgacaga agcactagc attgatttg aatgcccgc catggctcc gtattggcc atcggcgaa atcatccat gccttggct ttaactggc gacaagtcg atcctagcc ggccatctt gataaaatc gcaaacttg gttccggaa tttcggcaa aaattctac ttccgaggc ttaggaggc gaattaatg cgaacggcg tgttgcgat tttatcgag aagtgtgcc atggcgtcg ctcgaattc accaatggg agtgtgaca gacgattgg ctcaacttg atcaacgaa tgtatcgca tacaatgtc accaacatc caaacaaac gcaattaag gcgctacca actgtcttg agtcgttat tacgccaat caaaattgc gaaagtttg gttaaagga tatatcgaa gagttgcaa aaaacgagt caacaggag gtccgaatg gggcacgcc ttggctttg ggggccctc ccgaaattt gtgttggag ccccaattg ggggctatc attgactcg ctaattgcc acgagtcaa gcgagtcca ttgacggtc aaatgggca gaaagtcgc cgagattct atcaaggct ttgacctca ttgagtgtg acaatggcc gaagatttg gcaaagtcg gatgttttg cgaattttt gacgttttt ttggccagt ttgaaggat tatacgcag gataagaga ggcgatgtg ggcgcatgg gttcgcgag gcggccatg ttgggcctc cagactctg gcttttaag gtcaaagtg tcaatcgac gtagccttg actacgcag atcgtagcc ggggtggcg cagcaggct gtggagcgc atcgaccgg acgcgcgca ttggctggt cgaattttt tacagtttc ctgtacaac gaccccacc atcccgaat atacctcac cacgacgaa ttgttgaaa atttttgaa aaaaccgag tgtgagacg ttgaactgg aattcggcg agtgatacg ttcccgaaa tttgtcgaa ttgttgcaa tttcccgaa tttagctac aatgtgatg ttggggttg gtttgcagt gtgggcggt ttgaccgaa actctggtc aaaaattcg agttcgtcg ctctttgcc tacatctca agagagaaa aagtgtaaa ggggtgggg gagattgat aggttggcc gacgttatc tataggatt tttgaggat aataggaaa aatgaccga attgtggtc ccgatgttc cgttttttg gataaactt ttcagctcg gggtgtata caacatttg gcggatgac ccgagtagc gatttttgt aaaaaaatc ctcaagttg gccatgaat gaattggtg gggtgcaaa gacgcgtat aaaattata gatggtatt aatcttttg gcgcagttt gttcaggtc aaaagtgag gcctgtcag tcggctgta gtccagttg agtattttt ttgtgtcat cgacaggct tatgtgagg aaatcgaca gcgtctatt ttatacgaa tcgctacta gtgtatgga gattctgct gtttttaat actgaaaat ttggacgaa gttatgcaa attttgagt aacacaaac tgggatgat ccggttgat gtggtcaaa cctgcgagg aatgaaatg tgtaaatta atgggggtc cgagtgcct gttgccaaa acttccact aactaa
- >TC011056 RA
taaaacagt agtcgaaac ttaacctca caatttcta atttttcgt gacatctgt aaacaaaag accttttta tcgcaaaaa catgtcaac aagtgaaaa aaacaagcc ttcagatat ggaagagga gcctttcgg tcttggctg cgcccttga attgttcgt cgaagcgac cgaagtttc agaaatgat agacaccct taaatccat ttattcgga taaagccct caatgagaa ggcgtacga gcgttttag cttcatcat tggtcagta taaagaaca gccccactt gctggaccc ccatattga ccacctttt ggaaaaatg tatcaatat cgtacgaga tgggggctc cagtatgga cttgaaaca cgaagtttt taagtatat gttcgtgtt tgttaacgt gagggggta caaagtgat agtcaggca tcttccaca tgaggtttc agattttga agccgtttt aagactttt agaatcaca agactgtga tgattcaat cacttggac cactagata tgtactatt gttgtggtt atcaataat tgtcatgat tccgtttca cttatcacg atttgatgc aaatgatga aagcgagaa agagaagaa aacagtgat gtgtagggt tttggacat tatcaagaa atatattgt ggttcctga taagtgcag ggacgctgc ggcttatct ctcgtcgcg ttttatcac aagaaatga cgtcaaaca ggagcacct attgtctta catcgagtg gtcaatggt tgaaagtac ctcctccga gagtactat tttcacaaa acaaggaac tttagcctc aatcgccat gattttgaa aaccggaaa acgcgacga tttactccc tcatgcccg tcccctcct ccaatggat aatccgtga cgaaataaa acataattc cggtaccaa cgtccaaaa acttgtcta taaaatcgt ccaaagaat aggtttgac gtttctacc tcccaaagt ggccgcttg gcgctacaa acgcggcaa tcgttcatt ggctgcgaa tttaagtgg gggtgatgg taccacctc ctctcacga cacgcaaga acccctccc agaagataa tattgaggt tcctgatga agtcgagga ggttataga ccagttaat taacggttt gaggagttg tgatgggat tgtgcgatg gtcggctgc aaagggagt aggccgggt aaccggtcg gttacctca agaactggc cgatgaagt ggtcggttc ggttttgga gttgtttag tccgagaga gggggatgg ggcgtggca tggcggctg tttggcatt ggctgaatt ggggaggag gggcttgtt gctaccgaa acggttacc ggaggtggt accggttgt acttaaggc gttggtcta tgatgaacc gagaggata ttcctctgt aggatcaca tataaggga tgcagcgtg ttatgtgtg ttgggcctt tgcaagggc ctatgataa ggatgtttt aaaaccttt tgttaatca gatagctgc gaatttgtt gatcgtcgc ttgtttcga cagagagat caattgtcg acgagcggc ctctgccgc ctttcaaga aaacgtcgg acgtcaagg aacattccc gtacggaat tgacatttt gacagttgc tgacttttt cactgtcag tgtgcgaaa caacgccta cttgaccat aagtccgta catcgccca attcggcga atacaccat cccaatgat cgaccatct agttggccg aaaagtcga ccactggga ttgcgtcat tcgggaatt gacagccaa agctttgca taatttgac accaaaagc gcctgaata tatggtaac aaaagtgtt accaacctt gttcgacag aagcactag cattgattt gaatgcccg ccatggctc cgtattggc catcggcga aatcatcca tgccttggc tttaactgg cgacaagtc gatcctagc cggccatct tgataaaat cgcaaactt ggttccgga atttcggca aaaattcta cttccgagg cttaggagg cgaattaat gcgaacggc gtgttgcga ttttatcga gaagtgtgc catggcgtc gctcgaatt caccaatgg gagtgtgac agacgattg gctcaactt gatcaacga atgtatcgc atacaatgt caccaacat ccaaacaaa cgcaattaa ggcgctacc aactgtctt gagtcgtta ttacgccaa tcaaaattg cgaaagttt ggttaaagg atatatcga agagttgca aaaaacgag tcaacagga ggtccgaat ggggcacgc cttggcttt gggggccct cccgaaatt tgtgttgga gccccaatt gggggctat cattgactc gctaattgc cacgagtca agcgagtcc attgacggt caaatgggc agaaagtcg ccgagattc tatcaaggc tttgacctc attgagtgt gacaatggc cgaagattt ggcaaagtc ggatgtttt gcgaatttt tgacgtttt tttggccag tttgaagga ttatacgca ggataagag aggcgatgt gggcgcatg ggttcgcga ggcggccat gttgggcct ccagactct ggcttttaa ggtcaaagt gtcaatcga cgtagcctt gactacgca gatcgtagc cggggtggc gcagcaggc tgtggagcg catcgaccg gacgcgcgc attggctgg tcgaatttt ttacagttt cctgtacaa cgaccccac catcccgaa tatacctca ccacgacga attgttgaa aatttttga aaaaaccga gtgtgagac gttgaactg gaattcggc gagtgatac gttcccgaa atttgtcga attgttgca atttcccga atttagcta caatgtgat gttggggtt ggtttgcag tgtgggcgg tttgaccga aactctggt caaaaattc gagttcgtc gctctttgc ctacatctc aagagagaa aaagtgtaa aggggtggg ggagattga taggttggc cgacgttat ctataggat ttttgagga taataggaa aaatgaccg aattgtggt cccgatgtt ccgtttttt ggataaact tttcagctc ggggtgtat acaacattt ggcggatga cccgagtag cgatttttg taaaaaaat cctcaagtt ggccatgaa tgaattggt ggggtgcaa agacgcgta taaaattat agatggtat taatctttt ggcgcagtt tgttcaggt caaaagtga ggcctgtca gtcggctgt agtccagtt gagtatttt tttgtgtca tcgacaggc ttatgtgag gaaatcgac agcgtctat tttatacga atcgctact agtgtatgg agattctgc tgtttttaa tactgaaaa tttggacga agttatgca aattttgag taacacaaa ctgggatga tccggttga tgtggtcaa acctgcgag gaatgaaat gtgtaaatt aatgggggt ccgagtgcc tgttgccaa aacttccac taactaaat gatcagtgc tttttatat ttattaatt tttactatt gtgttacta aataaataa tacggaaag ta
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
