Status
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Gene information
General information
TC011033 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 6268100..6319089
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011033
    iBeetle screen iB_04964

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature degenerated - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    antenna partially size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head partially size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax dorsal not closed - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Pupal morphology
    wings slightly size shortened - Number of animals showing the phenotype: 8

    wings not closed - Number of animals showing the phenotype: 8
    Adult morphology
    elytra surface irregular - Number of animals showing the phenotype: 5


    elytra slightly size shortened - Number of animals showing the phenotype: 5


    hindwing folding irregular - Number of animals showing the phenotype: 2


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011033 RA
      MDVTFTNVL EGARQYFQG LPTATSSTA GPTYPNQSD 
      NLMRNDTEQ QQNEMPPST AAYWPQEPT RDRPPSRPS 
      YHQGSYQEQ QYHQFNRPQ SREAPSNQY HQYQRTPVV 
      TTQNYHQLQ NVGQGYYQQ QPQQQRTYY QMQAKSQQS 
      TQQNPYYKH LYAQSTNQM QYGGAKVEN KQEQVANQT 
      TSQAYRTTH NLPPIASLS LISYHSSRS NRDVIRTSS 
      TKTRTLPTN SMNQMPKSS TSNVQRYPE EYRTQYSNN 
      QNRLPQNYQ YGTTTTTTT QNYTQVIMT TSNYTAPVP 
      PTQSSQQYY QQQTNKIPN QNINGRVVP VIKQKRESP 
      LDLSVKTVR TPADSTLDD ADQDRKYYN RTTSHSYPT 
      YDVYANSFQ RNMTSRAPQ MPASAPKVE FHPNFNTQM 
      NNYRRTPTT SASTNRYPV VNNPYVQAK LPRVDFPPA 
      GHGHKTAAL YPVATHDTQ KKRPADTAP SIIPNKMSK 
      LDTWRQSID QQIEQRLSS YKQQQQAKK MVNGQERPK 
      EVYGYQNQN RQYQYPNQY HNQTYVPSA SAHQYPGYQ 
      YPTQQQVYN LPQNNPSVS QVVNKNPNL GGADKRVLS 
      LLRNSLEIK GAKKLEQEQ MNRSYDQLH KSRLDVQHP 
      STDVTAPLQ PKPGFINRH NVSPFTPTN LPDNNNTMY 
      KLHIPKAID SVNFEPETD SQVLITNHV NPNGDLDGL 
      AALVAARIR TKAELKQVS NNHHQRDTT GTPPKLNKE 
      KQAMAPRRR LFSRNEDDG GTNVPPRDK TGLRSSSET 
      SVFDFPDTD SEGEMPVLE RQTLEDMRR DRRSSTKTT 
      EQPRPLTPD DVFTQACDS FLQQLKNGS GKKRGRRKK 
      IEADVLAKL ETVVTKPTI KKEDDDDSD APLINCKKP 
      DKQLSVSLN DLTQLKAKK DVPIVPRPA KKPPFGDGS 
      HFYPGWEEE VYRYKKSLR MPPSLIHVT RPPQYHRLS 
      TSLPDLDPN SPITKIKSE NLDSDTESN HSFNLFSRH 
      NYDSEGSSS IKSCPNTAK ESSSILDKL LERCGGGAL 
      ATPARDRKT TLENFKNVF LASCKKRIK QRVTIKEVF 
      GEDRPASAP PVTCVDNIK IKEEPDDKK EDCEFGDTR 
      QVLKNKLLS RGRKESLLK SLVDKKIKS ELLDEEEEE 
      EELDSKSET PSIDGDEGG LGGKKRNKF RNIRRKFSS 
      GFDYIRKKK KSVKRDQEN EIKPKRRGQ FAAKGSPGS 
      VQDIQKEIK GWVLNKGIG ETILHRAAR LGYTDITAY 
      CLEKMEYSP SPKDNAGYT PLHEASTKG HLDIAKMLL 
      LYGANVSES ARGGIRPLH EAVENGYVE IVRLLLSYG 
      ADPKLATYS GMTPLALAS DDTTTKLLQ SHINDVEGE 
      PGAPWDFAG PASCFDPKE SGFDVLEDV PAPDIVSEE 
      DDLEFEVSD GVLPNLYTL RGESATDRW ILLQDLSSL 
      LKIKSRDAL LRQICPSGS SATTHVNYK SVLRELKMS 
      DFLEQAHCC QFLNIGEKI NTRASKIAL VKYTDKVRE 
      LLNVERVMI SVR
    • >TC011033 RB
      MPRLTRDSF FPLISWNRS RCNLQVMDV TFTNVLEGA 
      RQYFQGLPT ATSSTAGPT YPNQSDNLM RNDTEQQQN 
      EMPPSTAAY WPQEPTRDR PPSRPSYHQ GSYQEQQYH 
      QFNRPQSRE APSNQYHQY QRTPVVTTQ NYHQLQNVG 
      QGYYQQQPQ QQRTYYQMQ AKSQQSTQQ NPYYKHLYA 
      QSTNQMQYG GAKVENKQE QVANQTTSQ AYRTTHNLP 
      PIASLSLIS YHSSRSNRD VIRTSSTKT RTLPTNSMN 
      QMPKSSTSN VQRYPEEYR TQYSNNQNR LPQNYQYGT 
      TTTTTTQNY TQVIMTTSN YTAPVPPTQ SSQQYYQQQ 
      TNKIPNQNI NGRVVPVIK QKRESPLDL SVKTVRTPA 
      DSTLDDADQ DRKYYNRTT SHSYPTYDV YANSFQRNM 
      TSRAPQMPA SAPKVEFHP NFNTQMNNY RRTPTTSAS 
      TNRYPVVNN PYVQAKLPR VDFPPAGHG HKTAALYPV 
      ATHDTQKKR PADTAPSII PNKMSKLDT WRQSIDQQI 
      EQRLSSYKQ QQQAKKMVN GQERPKEVY GYQNQNRQY 
      QYPNQYHNQ TYVPSASAH QYPGYQYPT QQQVYNLPQ 
      NNPSVSQVV NKNPNLGGA DKRVLSLLR NSLEIKGAK 
      KLEQEQMNR SYDQLHKSR LDVQHPSTD VTAPLQPKP 
      GFINRHNVS PFTPTNLPD NNNTMYKLH IPKAIDSVN 
      FEPETDSQV LITNHVNPN GDLDGLAAL VAARIRTKA 
      ELKQVSNNH HQRDTTGTP PKLNKEKQA MAPRRRLFS 
      RNEDDGGTN VPPRDKTGL RSSSETSVF DFPDTDSEG 
      EMPVLERQT LEDMRRDRR SSTKTTEQP RPLTPDDVF 
      TQACDSFLQ QLKNGSGKK RGRRKKIEA DVLAKLETV 
      VTKPTIKKE DDDDSDAPL INCKKPDKQ LSVSLNDLT 
      QLKAKKDVP IVPRPAKKP PFGDGSHFY PGWEEEVYR 
      YKKSLRMPP SLIHVTRPP QYHRLSTSL PDLDPNSPI 
      TKIKSENLD SDTESNHSF NLFSRHNYD SEGSSSIKS 
      CPNTAKESS SILDKLLER CGGGALATP ARDRKTTLE 
      NFKNVFLAS CKKRIKQRV TIKEVFGED RPASAPPVT 
      CVDNIKIKE EPDDKKEDC EFGDTRQVL KNKLLSRGR 
      KESLLKSLV DKKIKSELL DEEEEEEEL DSKSETPSI 
      DGDEGGLGG KKRNKFRNI RRKFSSGFD YIRKKKKSV 
      KRDQENEIK PKRRGQFAA KGSPGSVQD IQKEIKGWV 
      LNKGIGETI LHRAARLGY TDITAYCLE KMEYSPSPK 
      DNAGYTPLH EASTKGHLD IAKMLLLYG ANVSESARG 
      GIRPLHEAV ENGYVEIVR LLLSYGADP KLATYSGMT 
      PLALASDDT TTKLLQSHI NDVEGEPGA PWDFAGPAS 
      CFDPKESGF DVLEDVPAP DIVSEEDDL EFEVSDGVL 
      PNLYTLRGE SATDRWILL QDLSSLLKI KSRDALLRQ 
      ICPSGSSAT THVNYKSVL RELKMSDFL EQAHCCQFL 
      NIGEKINTR ASKIALVKY TDKVRELLN VERVMISVR
    CDS sequence
    • >TC011033 RA
      atggatgtt accttcaca aacgtgttg gagggggcg 
      cgtcaatat ttccagggc ctgccaacg gccacgagc 
      tccacggcg ggccccacg tatccgaac cagtccgac 
      aacctcatg cgtaatgac accgaacag cagcagaac 
      gagatgccc ccatcgacg gcggcctac tggccccag 
      gagcccact cgggaccgg cccccttcc aggccctcc 
      taccaccag gggtcctac caggaacag cagtaccac 
      cagttcaac cggccgcag tcgcgcgaa gctccatcc 
      aaccagtac caccagtac cagcgaaca ccggtggtg 
      accacgcaa aactaccac cagttgcag aatgttggg 
      caagggtac taccagcaa cagccgcaa caacaaagg 
      acgtactac caaatgcaa gcaaagagt cagcaaagt 
      acgcagcaa aacccctat tataagcac ttgtacgcg 
      caaagtacc aatcagatg cagtatggt ggcgctaaa 
      gtcgagaac aagcaagaa caagtggcc aatcagacg 
      acgtcacaa gcgtacagg acaacgcat aaccttcca 
      ccaatcgcg tctttatct ttgataagt taccattcc 
      agtaggagt aacagagat gtgataaga acgtccagt 
      accaaaaca cgtaccttg ccgacgaat tcaatgaat 
      caaatgccc aaaagtagt acttccaac gttcagagg 
      tacccggag gagtaccga actcaatac tctaataat 
      caaaacaga cttccccaa aactatcag tacggtacc 
      accaccacc accaccacc caaaactac acacaagtg 
      atcatgaca acttccaat tacacggcc ccggtacca 
      ccgacccag tcttcccaa cagtactac caacaacaa 
      actaacaaa attcccaat caaaacatc aacggacga 
      gtggtacca gtcatcaaa caaaaacgc gaatctcca 
      cttgacttg tccgtgaaa acggtccga accccagcc 
      gactccacc cttgacgat gccgaccaa gaccgaaaa 
      tactacaac cggacaacc agccacagt tacccaact 
      tacgacgtt tacgccaac tcattccaa agaaatatg 
      acttcacga gcgccccaa atgcctgcc agcgccccc 
      aaagtcgaa ttccatccc aactttaac acccaaatg 
      aacaactat cgacgaact ccaactact agtgctagt 
      actaaccgg taccccgtc gtgaacaac ccatacgtc 
      caggccaaa ctaccacgg gttgacttc ccaccggct 
      gggcatggc cacaaaacc gcagccctg tacccagta 
      gccacccat gacacccag aagaaacga ccagcggac 
      acagcaccg tccatcata cccaacaaa atgtcaaaa 
      ttggacacc tggcggcaa agtatcgac caacaaatc 
      gagcaaaga ctatcgtcc tataaacaa caacaacaa 
      gccaagaag atggtcaat gggcaggaa cggcccaag 
      gaagtgtac gggtaccag aaccaaaac cggcagtac 
      cagtacccg aaccagtac cataaccag acttacgta 
      ccatcggca agcgcccat cagtacccg gggtaccag 
      taccccacc caacaacaa gtctataac ttaccacaa 
      aacaaccca tcggtgtca caagttgtt aacaaaaat 
      cctaatcta ggaggcgcc gataaaagg gtgctgtcg 
      ctactccgt aacagtctc gagatcaaa ggtgccaag 
      aaactcgaa caggaacaa atgaaccgg tcatatgac 
      caactccac aagtcccga ttggacgtc cagcacccc 
      tccactgac gtcacggcc cccctccaa cccaagccc 
      ggcttcatc aaccgccac aacgtctcc cccttcacc 
      cccaccaac ctccccgac aacaacaac accatgtac 
      aaactccac atccccaaa gccatcgac tcggtgaat 
      ttcgagccg gaaacagac agtcaagtc ctcatcacc 
      aaccatgtc aaccccaac ggtgacctt gacggcctt 
      gcagcgctc gtagcggcc cgcatacgc accaaagcc 
      gaactcaaa caagtctcc aacaaccac caccaacgc 
      gacacaacc gggactccc cccaaactc aacaaggag 
      aaacaagca atggcgcca cgccgccgc ctattcagt 
      cgcaacgag gacgatggc ggaaccaac gtccccccg 
      agagacaag actgggctg agaagttca tcggaaact 
      tcagttttt gatttcccc gacaccgac tccgagggg 
      gaaatgccg gtgttagaa cgccaaaca ctggaggac 
      atgagacgc gaccgccga tcctcaacg aaaacaacc 
      gaacagcca cgccctcta acccccgac gatgttttc 
      acacaagct tgtgacagc tttctccaa caattgaaa 
      aacggaagt gggaaaaaa cgtggccgg aggaaaaaa 
      atcgaagct gatgttttg gctaaactc gagactgtc 
      gtcacgaaa ccgaccatc aaaaaagag gacgacgac 
      gattcagac gcacctttg atcaattgc aaaaaaccc 
      gacaaacaa ttgagcgtg tccttgaac gacttgacc 
      cagctgaaa gccaagaag gacgtcccg attgtccca 
      cgaccggcc aagaagcca ccctttggg gacggctcc 
      cacttttac cccggctgg gaggaggag gtctaccgc 
      tacaagaag tcgctccgg atgcccccc agtttaatc 
      cacgtgaca cgcccccca caataccac cgcttgtcc 
      acctccctg cccgacttg gaccccaac tccccaatc 
      accaaaatc aagagcgag aatctggac agcgacacg 
      gaatccaac cacagtttc aatctcttc agcagacac 
      aactacgac tctgaaggc agttcgtcc attaagagt 
      tgtccaaac acggccaaa gaaagcagc tcgattttg 
      gacaagttg ttggagcgt tgcggcggt ggagctttg 
      gcgacgccg gctcgtgac cgcaaaacc acgcttgag 
      aatttcaaa aacgtgttt ttggcgagt tgcaagaag 
      aggatcaag cagcgagtg acgattaag gaggtgttc 
      ggggaggat cggccggcg tcggcgccg cctgtcacc 
      tgtgttgac aatattaag atcaaggag gagcccgat 
      gacaagaag gaggattgc gagtttggg gatacgagg 
      caagtgctc aagaacaag ttgctgagt cggggccgc 
      aaggagagt ctgttgaag agtctggtg gataagaag 
      atcaagtcg gagttgttg gatgaggag gaggaagag 
      gaggagctg gatagcaag tcggagacg ccgtcgatt 
      gatggggat gaagggggt ttggggggg aagaaacgt 
      aacaagttt aggaacatt agacgtaaa tttagttca 
      gggtttgat tatatacgt aagaagaag aagagtgtg 
      aagagggat caggagaat gagattaag cccaaacga 
      agaggacag tttgcggcg aaggggagt ccagggtcg 
      gtgcaagat atacagaag gagattaag ggatgggtt 
      ttgaacaag gggattggg gagacgata ctgcacagg 
      gcggcacgg ctgggatac acggacatt accgcctat 
      tgtctcgaa aaaatggaa tattcgccg tcgccgaaa 
      gacaacgct ggctacacg cccttgcac gaagcgagc 
      acaaagggc cacttggac atcgccaaa atgttgctt 
      ctctacggc gcgaacgtg agcgaatcg gcccgaggt 
      ggcatcaga cctttgcac gaagccgtc gaaaacgga 
      tacgtggaa atcgtgcgc ttattacta tcctacgga 
      gccgacccc aaactggcc acttactcc ggcatgacc 
      cccttggcc ctcgcctcc gacgacacg accaccaaa 
      ctcctccaa agccacatc aacgacgtg gagggggag 
      ccgggcgcc ccttgggac tttgcagga ccggcttct 
      tgtttcgac ccgaaagag agcggtttc gacgtgctg 
      gaggacgtg cccgccccg gacatcgtc tccgaggag 
      gatgacctg gagttcgag gtttcggac ggggttttg 
      cccaacttg tacacgctt cggggcgag tcggccacc 
      gaccggtgg atcctcctc caggacttg tccagctta 
      ttgaagatc aaatcgcgg gacgcgttg ttgagacag 
      atttgtccg tcgggttcg agtgccacg acgcacgtg 
      aattacaag agtgttttg cgggaactg aaaatgtcg 
      gactttctg gagcaggcc cactgctgc cagttcctc 
      aatatcggg gaaaagata aacacgcgc gcttcgaag 
      atcgccttg gtcaagtat acggacaaa gtgagggaa 
      ctgttgaac gtcgaaagg gttatgatt tcggtcagg 
      tga
    • >TC011033 RB
      atgcccagg ctaacccgg gactctttt tttcctctc 
      ataagttgg aatcgcagc aggtgcaac ctgcaggtc 
      atggatgtt accttcaca aacgtgttg gagggggcg 
      cgtcaatat ttccagggc ctgccaacg gccacgagc 
      tccacggcg ggccccacg tatccgaac cagtccgac 
      aacctcatg cgtaatgac accgaacag cagcagaac 
      gagatgccc ccatcgacg gcggcctac tggccccag 
      gagcccact cgggaccgg cccccttcc aggccctcc 
      taccaccag gggtcctac caggaacag cagtaccac 
      cagttcaac cggccgcag tcgcgcgaa gctccatcc 
      aaccagtac caccagtac cagcgaaca ccggtggtg 
      accacgcaa aactaccac cagttgcag aatgttggg 
      caagggtac taccagcaa cagccgcaa caacaaagg 
      acgtactac caaatgcaa gcaaagagt cagcaaagt 
      acgcagcaa aacccctat tataagcac ttgtacgcg 
      caaagtacc aatcagatg cagtatggt ggcgctaaa 
      gtcgagaac aagcaagaa caagtggcc aatcagacg 
      acgtcacaa gcgtacagg acaacgcat aaccttcca 
      ccaatcgcg tctttatct ttgataagt taccattcc 
      agtaggagt aacagagat gtgataaga acgtccagt 
      accaaaaca cgtaccttg ccgacgaat tcaatgaat 
      caaatgccc aaaagtagt acttccaac gttcagagg 
      tacccggag gagtaccga actcaatac tctaataat 
      caaaacaga cttccccaa aactatcag tacggtacc 
      accaccacc accaccacc caaaactac acacaagtg 
      atcatgaca acttccaat tacacggcc ccggtacca 
      ccgacccag tcttcccaa cagtactac caacaacaa 
      actaacaaa attcccaat caaaacatc aacggacga 
      gtggtacca gtcatcaaa caaaaacgc gaatctcca 
      cttgacttg tccgtgaaa acggtccga accccagcc 
      gactccacc cttgacgat gccgaccaa gaccgaaaa 
      tactacaac cggacaacc agccacagt tacccaact 
      tacgacgtt tacgccaac tcattccaa agaaatatg 
      acttcacga gcgccccaa atgcctgcc agcgccccc 
      aaagtcgaa ttccatccc aactttaac acccaaatg 
      aacaactat cgacgaact ccaactact agtgctagt 
      actaaccgg taccccgtc gtgaacaac ccatacgtc 
      caggccaaa ctaccacgg gttgacttc ccaccggct 
      gggcatggc cacaaaacc gcagccctg tacccagta 
      gccacccat gacacccag aagaaacga ccagcggac 
      acagcaccg tccatcata cccaacaaa atgtcaaaa 
      ttggacacc tggcggcaa agtatcgac caacaaatc 
      gagcaaaga ctatcgtcc tataaacaa caacaacaa 
      gccaagaag atggtcaat gggcaggaa cggcccaag 
      gaagtgtac gggtaccag aaccaaaac cggcagtac 
      cagtacccg aaccagtac cataaccag acttacgta 
      ccatcggca agcgcccat cagtacccg gggtaccag 
      taccccacc caacaacaa gtctataac ttaccacaa 
      aacaaccca tcggtgtca caagttgtt aacaaaaat 
      cctaatcta ggaggcgcc gataaaagg gtgctgtcg 
      ctactccgt aacagtctc gagatcaaa ggtgccaag 
      aaactcgaa caggaacaa atgaaccgg tcatatgac 
      caactccac aagtcccga ttggacgtc cagcacccc 
      tccactgac gtcacggcc cccctccaa cccaagccc 
      ggcttcatc aaccgccac aacgtctcc cccttcacc 
      cccaccaac ctccccgac aacaacaac accatgtac 
      aaactccac atccccaaa gccatcgac tcggtgaat 
      ttcgagccg gaaacagac agtcaagtc ctcatcacc 
      aaccatgtc aaccccaac ggtgacctt gacggcctt 
      gcagcgctc gtagcggcc cgcatacgc accaaagcc 
      gaactcaaa caagtctcc aacaaccac caccaacgc 
      gacacaacc gggactccc cccaaactc aacaaggag 
      aaacaagca atggcgcca cgccgccgc ctattcagt 
      cgcaacgag gacgatggc ggaaccaac gtccccccg 
      agagacaag actgggctg agaagttca tcggaaact 
      tcagttttt gatttcccc gacaccgac tccgagggg 
      gaaatgccg gtgttagaa cgccaaaca ctggaggac 
      atgagacgc gaccgccga tcctcaacg aaaacaacc 
      gaacagcca cgccctcta acccccgac gatgttttc 
      acacaagct tgtgacagc tttctccaa caattgaaa 
      aacggaagt gggaaaaaa cgtggccgg aggaaaaaa 
      atcgaagct gatgttttg gctaaactc gagactgtc 
      gtcacgaaa ccgaccatc aaaaaagag gacgacgac 
      gattcagac gcacctttg atcaattgc aaaaaaccc 
      gacaaacaa ttgagcgtg tccttgaac gacttgacc 
      cagctgaaa gccaagaag gacgtcccg attgtccca 
      cgaccggcc aagaagcca ccctttggg gacggctcc 
      cacttttac cccggctgg gaggaggag gtctaccgc 
      tacaagaag tcgctccgg atgcccccc agtttaatc 
      cacgtgaca cgcccccca caataccac cgcttgtcc 
      acctccctg cccgacttg gaccccaac tccccaatc 
      accaaaatc aagagcgag aatctggac agcgacacg 
      gaatccaac cacagtttc aatctcttc agcagacac 
      aactacgac tctgaaggc agttcgtcc attaagagt 
      tgtccaaac acggccaaa gaaagcagc tcgattttg 
      gacaagttg ttggagcgt tgcggcggt ggagctttg 
      gcgacgccg gctcgtgac cgcaaaacc acgcttgag 
      aatttcaaa aacgtgttt ttggcgagt tgcaagaag 
      aggatcaag cagcgagtg acgattaag gaggtgttc 
      ggggaggat cggccggcg tcggcgccg cctgtcacc 
      tgtgttgac aatattaag atcaaggag gagcccgat 
      gacaagaag gaggattgc gagtttggg gatacgagg 
      caagtgctc aagaacaag ttgctgagt cggggccgc 
      aaggagagt ctgttgaag agtctggtg gataagaag 
      atcaagtcg gagttgttg gatgaggag gaggaagag 
      gaggagctg gatagcaag tcggagacg ccgtcgatt 
      gatggggat gaagggggt ttggggggg aagaaacgt 
      aacaagttt aggaacatt agacgtaaa tttagttca 
      gggtttgat tatatacgt aagaagaag aagagtgtg 
      aagagggat caggagaat gagattaag cccaaacga 
      agaggacag tttgcggcg aaggggagt ccagggtcg 
      gtgcaagat atacagaag gagattaag ggatgggtt 
      ttgaacaag gggattggg gagacgata ctgcacagg 
      gcggcacgg ctgggatac acggacatt accgcctat 
      tgtctcgaa aaaatggaa tattcgccg tcgccgaaa 
      gacaacgct ggctacacg cccttgcac gaagcgagc 
      acaaagggc cacttggac atcgccaaa atgttgctt 
      ctctacggc gcgaacgtg agcgaatcg gcccgaggt 
      ggcatcaga cctttgcac gaagccgtc gaaaacgga 
      tacgtggaa atcgtgcgc ttattacta tcctacgga 
      gccgacccc aaactggcc acttactcc ggcatgacc 
      cccttggcc ctcgcctcc gacgacacg accaccaaa 
      ctcctccaa agccacatc aacgacgtg gagggggag 
      ccgggcgcc ccttgggac tttgcagga ccggcttct 
      tgtttcgac ccgaaagag agcggtttc gacgtgctg 
      gaggacgtg cccgccccg gacatcgtc tccgaggag 
      gatgacctg gagttcgag gtttcggac ggggttttg 
      cccaacttg tacacgctt cggggcgag tcggccacc 
      gaccggtgg atcctcctc caggacttg tccagctta 
      ttgaagatc aaatcgcgg gacgcgttg ttgagacag 
      atttgtccg tcgggttcg agtgccacg acgcacgtg 
      aattacaag agtgttttg cgggaactg aaaatgtcg 
      gactttctg gagcaggcc cactgctgc cagttcctc 
      aatatcggg gaaaagata aacacgcgc gcttcgaag 
      atcgccttg gtcaagtat acggacaaa gtgagggaa 
      ctgttgaac gtcgaaagg gttatgatt tcggtcagg 
      tga
    mRNA sequence
    • >TC011033 RA
      atttttctt ttatttttc aggatgccg tcaagcttt 
      gacaaaatg ccggccctg cgctggctg cttgtaaca 
      gcaggctaa cccgggact ctttttttc ctctcataa 
      gttggaatc gcagcaggt gcaacctgc aggtcatgg 
      atgttacct tcacaaacg tgttggagg gggcgcgtc 
      aatatttcc agggcctgc caacggcca cgagctcca 
      cggcgggcc ccacgtatc cgaaccagt ccgacaacc 
      tcatgcgta atgacaccg aacagcagc agaacgaga 
      tgcccccat cgacggcgg cctactggc cccaggagc 
      ccactcggg accggcccc cttccaggc cctcctacc 
      accaggggt cctaccagg aacagcagt accaccagt 
      tcaaccggc cgcagtcgc gcgaagctc catccaacc 
      agtaccacc agtaccagc gaacaccgg tggtgacca 
      cgcaaaact accaccagt tgcagaatg ttgggcaag 
      ggtactacc agcaacagc cgcaacaac aaaggacgt 
      actaccaaa tgcaagcaa agagtcagc aaagtacgc 
      agcaaaacc cctattata agcacttgt acgcgcaaa 
      gtaccaatc agatgcagt atggtggcg ctaaagtcg 
      agaacaagc aagaacaag tggccaatc agacgacgt 
      cacaagcgt acaggacaa cgcataacc ttccaccaa 
      tcgcgtctt tatctttga taagttacc attccagta 
      ggagtaaca gagatgtga taagaacgt ccagtacca 
      aaacacgta ccttgccga cgaattcaa tgaatcaaa 
      tgcccaaaa gtagtactt ccaacgttc agaggtacc 
      cggaggagt accgaactc aatactcta ataatcaaa 
      acagacttc cccaaaact atcagtacg gtaccacca 
      ccaccacca ccacccaaa actacacac aagtgatca 
      tgacaactt ccaattaca cggccccgg taccaccga 
      cccagtctt cccaacagt actaccaac aacaaacta 
      acaaaattc ccaatcaaa acatcaacg gacgagtgg 
      taccagtca tcaaacaaa aacgcgaat ctccacttg 
      acttgtccg tgaaaacgg tccgaaccc cagccgact 
      ccacccttg acgatgccg accaagacc gaaaatact 
      acaaccgga caaccagcc acagttacc caacttacg 
      acgtttacg ccaactcat tccaaagaa atatgactt 
      cacgagcgc cccaaatgc ctgccagcg cccccaaag 
      tcgaattcc atcccaact ttaacaccc aaatgaaca 
      actatcgac gaactccaa ctactagtg ctagtacta 
      accggtacc ccgtcgtga acaacccat acgtccagg 
      ccaaactac cacgggttg acttcccac cggctgggc 
      atggccaca aaaccgcag ccctgtacc cagtagcca 
      cccatgaca cccagaaga aacgaccag cggacacag 
      caccgtcca tcataccca acaaaatgt caaaattgg 
      acacctggc ggcaaagta tcgaccaac aaatcgagc 
      aaagactat cgtcctata aacaacaac aacaagcca 
      agaagatgg tcaatgggc aggaacggc ccaaggaag 
      tgtacgggt accagaacc aaaaccggc agtaccagt 
      acccgaacc agtaccata accagactt acgtaccat 
      cggcaagcg cccatcagt acccggggt accagtacc 
      ccacccaac aacaagtct ataacttac cacaaaaca 
      acccatcgg tgtcacaag ttgttaaca aaaatccta 
      atctaggag gcgccgata aaagggtgc tgtcgctac 
      tccgtaaca gtctcgaga tcaaaggtg ccaagaaac 
      tcgaacagg aacaaatga accggtcat atgaccaac 
      tccacaagt cccgattgg acgtccagc acccctcca 
      ctgacgtca cggcccccc tccaaccca agcccggct 
      tcatcaacc gccacaacg tctccccct tcaccccca 
      ccaacctcc ccgacaaca acaacacca tgtacaaac 
      tccacatcc ccaaagcca tcgactcgg tgaatttcg 
      agccggaaa cagacagtc aagtcctca tcaccaacc 
      atgtcaacc ccaacggtg accttgacg gccttgcag 
      cgctcgtag cggcccgca tacgcacca aagccgaac 
      tcaaacaag tctccaaca accaccacc aacgcgaca 
      caaccggga ctcccccca aactcaaca aggagaaac 
      aagcaatgg cgccacgcc gccgcctat tcagtcgca 
      acgaggacg atggcggaa ccaacgtcc ccccgagag 
      acaagactg ggctgagaa gttcatcgg aaacttcag 
      tttttgatt tccccgaca ccgactccg agggggaaa 
      tgccggtgt tagaacgcc aaacactgg aggacatga 
      gacgcgacc gccgatcct caacgaaaa caaccgaac 
      agccacgcc ctctaaccc ccgacgatg ttttcacac 
      aagcttgtg acagctttc tccaacaat tgaaaaacg 
      gaagtggga aaaaacgtg gccggagga aaaaaatcg 
      aagctgatg ttttggcta aactcgaga ctgtcgtca 
      cgaaaccga ccatcaaaa aagaggacg acgacgatt 
      cagacgcac ctttgatca attgcaaaa aacccgaca 
      aacaattga gcgtgtcct tgaacgact tgacccagc 
      tgaaagcca agaaggacg tcccgattg tcccacgac 
      cggccaaga agccaccct ttggggacg gctcccact 
      tttaccccg gctgggagg aggaggtct accgctaca 
      agaagtcgc tccggatgc cccccagtt taatccacg 
      tgacacgcc ccccacaat accaccgct tgtccacct 
      ccctgcccg acttggacc ccaactccc caatcacca 
      aaatcaaga gcgagaatc tggacagcg acacggaat 
      ccaaccaca gtttcaatc tcttcagca gacacaact 
      acgactctg aaggcagtt cgtccatta agagttgtc 
      caaacacgg ccaaagaaa gcagctcga ttttggaca 
      agttgttgg agcgttgcg gcggtggag ctttggcga 
      cgccggctc gtgaccgca aaaccacgc ttgagaatt 
      tcaaaaacg tgtttttgg cgagttgca agaagagga 
      tcaagcagc gagtgacga ttaaggagg tgttcgggg 
      aggatcggc cggcgtcgg cgccgcctg tcacctgtg 
      ttgacaata ttaagatca aggaggagc ccgatgaca 
      agaaggagg attgcgagt ttggggata cgaggcaag 
      tgctcaaga acaagttgc tgagtcggg gccgcaagg 
      agagtctgt tgaagagtc tggtggata agaagatca 
      agtcggagt tgttggatg aggaggagg aagaggagg 
      agctggata gcaagtcgg agacgccgt cgattgatg 
      gggatgaag ggggtttgg gggggaaga aacgtaaca 
      agtttagga acattagac gtaaattta gttcagggt 
      ttgattata tacgtaaga agaagaaga gtgtgaaga 
      gggatcagg agaatgaga ttaagccca aacgaagag 
      gacagtttg cggcgaagg ggagtccag ggtcggtgc 
      aagatatac agaaggaga ttaagggat gggttttga 
      acaagggga ttggggaga cgatactgc acagggcgg 
      cacggctgg gatacacgg acattaccg cctattgtc 
      tcgaaaaaa tggaatatt cgccgtcgc cgaaagaca 
      acgctggct acacgccct tgcacgaag cgagcacaa 
      agggccact tggacatcg ccaaaatgt tgcttctct 
      acggcgcga acgtgagcg aatcggccc gaggtggca 
      tcagacctt tgcacgaag ccgtcgaaa acggatacg 
      tggaaatcg tgcgcttat tactatcct acggagccg 
      accccaaac tggccactt actccggca tgaccccct 
      tggccctcg cctccgacg acacgacca ccaaactcc 
      tccaaagcc acatcaacg acgtggagg gggagccgg 
      gcgcccctt gggactttg caggaccgg cttcttgtt 
      tcgacccga aagagagcg gtttcgacg tgctggagg 
      acgtgcccg ccccggaca tcgtctccg aggaggatg 
      acctggagt tcgaggttt cggacgggg ttttgccca 
      acttgtaca cgcttcggg gcgagtcgg ccaccgacc 
      ggtggatcc tcctccagg acttgtcca gcttattga 
      agatcaaat cgcgggacg cgttgttga gacagattt 
      gtccgtcgg gttcgagtg ccacgacgc acgtgaatt 
      acaagagtg ttttgcggg aactgaaaa tgtcggact 
      ttctggagc aggcccact gctgccagt tcctcaata 
      tcggggaaa agataaaca cgcgcgctt cgaagatcg 
      ccttggtca agtatacgg acaaagtga gggaactgt 
      tgaacgtcg aaagggtta tgatttcgg tcaggtgac 
      ctacgaagc gggacacct agacgtctt ttccgtgat 
      atattcgat tgtttgtga ttgaaatta cctgtttga 
      tttttttcg gtgtaatat tttggtgct ttctagcca 
      ctaaacggt tttttattt gaccatgtt tcatgtttt 
      tagtttaga tatttatta tagagaatt ttaaataaa 
      ggttaggtt accaatgct tttttagat cattttaca 
      acttaggct tattcggtt ttcaataaa ctaattgta 
      atgagagtg tgcaattct tttgtatcg tcgagtaac 
      tagctttga atgtgtgat cctgttgaa gaaataaat 
      ttgtatact agaaattat tttatacag aaatgatac 
      tcacagtga atagtattg taagataat cgttatcgt 
      tgagctcag ggagatgta ctctctttc agagtacca 
      taaacaaat ctactgtct aactataaa tatttgtat 
      aaatgtata ctcagctag gatacagcg tgtacatat 
      tttcttgga catctttat gtatttttg ttatataag 
      gtgatagct tttcatttt ctatgtaat agacgatgt 
      tatgtcatg tgacttata tgtgtgctt gcttaccta 
      aagcaactc cactcgttt ttttttcgg cttttaata 
      gttgggtag ttgtactgt acagtaaag agttatctg 
      tgatgcgca tcggtggca aagtgcaat gcttttttt 
      gcatatgta ttaaaaaca tattgtgat tttgccaat 
      ttgtcctat ccgtgtgag ttagaaaag tatttttaa 
      tttataaat cttttttca catttttta tgttttctg 
      ttcgaaaaa catgtgaaa tattaataa accatatat 
      cgaaca
    • >TC011033 RB
      ttcggtgga agtcttgaa attgtttat ttttgcctg 
      gattgactt aattctttt ttcgttttt tccaggatt 
      gaatggaag tggcagtac ttaattaca tcgtcccag 
      ttttttaaa ggatgccca ggctaaccc gggactctt 
      tttttcctc tcataagtt ggaatcgca gcaggtgca 
      acctgcagg tcatggatg ttaccttca caaacgtgt 
      tggaggggg cgcgtcaat atttccagg gcctgccaa 
      cggccacga gctccacgg cgggcccca cgtatccga 
      accagtccg acaacctca tgcgtaatg acaccgaac 
      agcagcaga acgagatgc ccccatcga cggcggcct 
      actggcccc aggagccca ctcgggacc ggccccctt 
      ccaggccct cctaccacc aggggtcct accaggaac 
      agcagtacc accagttca accggccgc agtcgcgcg 
      aagctccat ccaaccagt accaccagt accagcgaa 
      caccggtgg tgaccacgc aaaactacc accagttgc 
      agaatgttg ggcaagggt actaccagc aacagccgc 
      aacaacaaa ggacgtact accaaatgc aagcaaaga 
      gtcagcaaa gtacgcagc aaaacccct attataagc 
      acttgtacg cgcaaagta ccaatcaga tgcagtatg 
      gtggcgcta aagtcgaga acaagcaag aacaagtgg 
      ccaatcaga cgacgtcac aagcgtaca ggacaacgc 
      ataaccttc caccaatcg cgtctttat ctttgataa 
      gttaccatt ccagtagga gtaacagag atgtgataa 
      gaacgtcca gtaccaaaa cacgtacct tgccgacga 
      attcaatga atcaaatgc ccaaaagta gtacttcca 
      acgttcaga ggtacccgg aggagtacc gaactcaat 
      actctaata atcaaaaca gacttcccc aaaactatc 
      agtacggta ccaccacca ccaccacca cccaaaact 
      acacacaag tgatcatga caacttcca attacacgg 
      ccccggtac caccgaccc agtcttccc aacagtact 
      accaacaac aaactaaca aaattccca atcaaaaca 
      tcaacggac gagtggtac cagtcatca aacaaaaac 
      gcgaatctc cacttgact tgtccgtga aaacggtcc 
      gaaccccag ccgactcca cccttgacg atgccgacc 
      aagaccgaa aatactaca accggacaa ccagccaca 
      gttacccaa cttacgacg tttacgcca actcattcc 
      aaagaaata tgacttcac gagcgcccc aaatgcctg 
      ccagcgccc ccaaagtcg aattccatc ccaacttta 
      acacccaaa tgaacaact atcgacgaa ctccaacta 
      ctagtgcta gtactaacc ggtaccccg tcgtgaaca 
      acccatacg tccaggcca aactaccac gggttgact 
      tcccaccgg ctgggcatg gccacaaaa ccgcagccc 
      tgtacccag tagccaccc atgacaccc agaagaaac 
      gaccagcgg acacagcac cgtccatca tacccaaca 
      aaatgtcaa aattggaca cctggcggc aaagtatcg 
      accaacaaa tcgagcaaa gactatcgt cctataaac 
      aacaacaac aagccaaga agatggtca atgggcagg 
      aacggccca aggaagtgt acgggtacc agaaccaaa 
      accggcagt accagtacc cgaaccagt accataacc 
      agacttacg taccatcgg caagcgccc atcagtacc 
      cggggtacc agtacccca cccaacaac aagtctata 
      acttaccac aaaacaacc catcggtgt cacaagttg 
      ttaacaaaa atcctaatc taggaggcg ccgataaaa 
      gggtgctgt cgctactcc gtaacagtc tcgagatca 
      aaggtgcca agaaactcg aacaggaac aaatgaacc 
      ggtcatatg accaactcc acaagtccc gattggacg 
      tccagcacc cctccactg acgtcacgg cccccctcc 
      aacccaagc ccggcttca tcaaccgcc acaacgtct 
      cccccttca cccccacca acctccccg acaacaaca 
      acaccatgt acaaactcc acatcccca aagccatcg 
      actcggtga atttcgagc cggaaacag acagtcaag 
      tcctcatca ccaaccatg tcaacccca acggtgacc 
      ttgacggcc ttgcagcgc tcgtagcgg cccgcatac 
      gcaccaaag ccgaactca aacaagtct ccaacaacc 
      accaccaac gcgacacaa ccgggactc cccccaaac 
      tcaacaagg agaaacaag caatggcgc cacgccgcc 
      gcctattca gtcgcaacg aggacgatg gcggaacca 
      acgtccccc cgagagaca agactgggc tgagaagtt 
      catcggaaa cttcagttt ttgatttcc ccgacaccg 
      actccgagg gggaaatgc cggtgttag aacgccaaa 
      cactggagg acatgagac gcgaccgcc gatcctcaa 
      cgaaaacaa ccgaacagc cacgccctc taacccccg 
      acgatgttt tcacacaag cttgtgaca gctttctcc 
      aacaattga aaaacggaa gtgggaaaa aacgtggcc 
      ggaggaaaa aaatcgaag ctgatgttt tggctaaac 
      tcgagactg tcgtcacga aaccgacca tcaaaaaag 
      aggacgacg acgattcag acgcacctt tgatcaatt 
      gcaaaaaac ccgacaaac aattgagcg tgtccttga 
      acgacttga cccagctga aagccaaga aggacgtcc 
      cgattgtcc cacgaccgg ccaagaagc caccctttg 
      gggacggct cccactttt accccggct gggaggagg 
      aggtctacc gctacaaga agtcgctcc ggatgcccc 
      ccagtttaa tccacgtga cacgccccc cacaatacc 
      accgcttgt ccacctccc tgcccgact tggacccca 
      actccccaa tcaccaaaa tcaagagcg agaatctgg 
      acagcgaca cggaatcca accacagtt tcaatctct 
      tcagcagac acaactacg actctgaag gcagttcgt 
      ccattaaga gttgtccaa acacggcca aagaaagca 
      gctcgattt tggacaagt tgttggagc gttgcggcg 
      gtggagctt tggcgacgc cggctcgtg accgcaaaa 
      ccacgcttg agaatttca aaaacgtgt ttttggcga 
      gttgcaaga agaggatca agcagcgag tgacgatta 
      aggaggtgt tcggggagg atcggccgg cgtcggcgc 
      cgcctgtca cctgtgttg acaatatta agatcaagg 
      aggagcccg atgacaaga aggaggatt gcgagtttg 
      gggatacga ggcaagtgc tcaagaaca agttgctga 
      gtcggggcc gcaaggaga gtctgttga agagtctgg 
      tggataaga agatcaagt cggagttgt tggatgagg 
      aggaggaag aggaggagc tggatagca agtcggaga 
      cgccgtcga ttgatgggg atgaagggg gtttggggg 
      ggaagaaac gtaacaagt ttaggaaca ttagacgta 
      aatttagtt cagggtttg attatatac gtaagaaga 
      agaagagtg tgaagaggg atcaggaga atgagatta 
      agcccaaac gaagaggac agtttgcgg cgaagggga 
      gtccagggt cggtgcaag atatacaga aggagatta 
      agggatggg ttttgaaca aggggattg gggagacga 
      tactgcaca gggcggcac ggctgggat acacggaca 
      ttaccgcct attgtctcg aaaaaatgg aatattcgc 
      cgtcgccga aagacaacg ctggctaca cgcccttgc 
      acgaagcga gcacaaagg gccacttgg acatcgcca 
      aaatgttgc ttctctacg gcgcgaacg tgagcgaat 
      cggcccgag gtggcatca gacctttgc acgaagccg 
      tcgaaaacg gatacgtgg aaatcgtgc gcttattac 
      tatcctacg gagccgacc ccaaactgg ccacttact 
      ccggcatga cccccttgg ccctcgcct ccgacgaca 
      cgaccacca aactcctcc aaagccaca tcaacgacg 
      tggaggggg agccgggcg ccccttggg actttgcag 
      gaccggctt cttgtttcg acccgaaag agagcggtt 
      tcgacgtgc tggaggacg tgcccgccc cggacatcg 
      tctccgagg aggatgacc tggagttcg aggtttcgg 
      acggggttt tgcccaact tgtacacgc ttcggggcg 
      agtcggcca ccgaccggt ggatcctcc tccaggact 
      tgtccagct tattgaaga tcaaatcgc gggacgcgt 
      tgttgagac agatttgtc cgtcgggtt cgagtgcca 
      cgacgcacg tgaattaca agagtgttt tgcgggaac 
      tgaaaatgt cggactttc tggagcagg cccactgct 
      gccagttcc tcaatatcg gggaaaaga taaacacgc 
      gcgcttcga agatcgcct tggtcaagt atacggaca 
      aagtgaggg aactgttga acgtcgaaa gggttatga 
      tttcggtca ggtgaccta cgaagcggg acacctaga 
      cgtcttttc cgtgatata ttcgattgt ttgtgattg 
      aaattacct gtttgattt ttttcggtg taatatttt 
      ggtgctttc tagccacta aacggtttt ttatttgac 
      catgtttca tgtttttag tttagatat ttattatag 
      agaatttta aataaaggt taggttacc aatgctttt 
      ttagatcat tttacaact taggcttat tcggttttc 
      aataaacta attgtaatg agagtgtgc aattctttt 
      gtatcgtcg agtaactag ctttgaatg tgtgatcct 
      gttgaagaa ataaatttg tatactaga aattatttt 
      atacagaaa tgatactca cagtgaata gtattgtaa 
      gataatcgt tatcgttga gctcaggga gatgtactc 
      tctttcaga gtaccataa acaaatcta ctgtctaac 
      tataaatat ttgtataaa tgtatactc agctaggat 
      acagcgtgt acatatttt cttggacat ctttatgta 
      tttttgtta tataaggtg atagctttt cattttcta 
      tgtaataga cgatgttat gtcatgtga cttatatgt 
      gtgcttgct tacctaaag caactccac tcgtttttt 
      tttcggctt ttaatagtt gggtagttg tactgtaca 
      gtaaagagt tatctgtga tgcgcatcg gtggcaaag 
      tgcaatgct ttttttgca tatgtatta aaaacatat 
      tgtgatttt gccaatttg tcctatccg tgtgagtta 
      gaaaagtat ttttaattt ataaatctt ttttcacat 
      tttttatgt tttctgttc gaaaaacat gtgaaatat 
      taataaacc atatatcga aca
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