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Gene information
General information
TC011010 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 6779962..6781598
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011010
    iBeetle screen iB_04963

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011010 RA
      MENVRTLDL DKICRTCLT ENKDMFSIF SQLYEVHSQ 
ESDIPCIDE ILSKISSIK AVRNDGFPS LICTKCIDL 
AHTSFNFQK LCNRSQNIL ETFVKQQPE TSIKLENVE 
KNEFLVVER ASDEESDKA DVPGQANEE EKNFACTIC 
EKSYKFANS LRVHMRNHS EEKPYECKT CGKCFKQYG 
SLVYHQRSH TGVQPYVCK TCGKKYKQS GTLTAHMRV 
HTGQRPFLC SICGRGFRQ AADLGYHMR SHTKEKPYM 
CNICGKTMS MQSHLVQHL RTHTGERPY KCDVCGKAF 
PSSTRLKRH AIIHTSLKP YTCEVCAKS FNRMSSLKV 
HVKVHTDER SHVCPICSK GFIQAQALR SHLGSHSSE 
LSVKPAQGF
    CDS sequence
    • >TC011010 RA
      atggaaaat gtgcggacg cttgacttg gataagatc 
tgtagaacg tgtctgact gaaaataag gacatgttt 
tcgattttt agccaattg tacgaagtg cactcacag 
gagagtgat attccgtgt attgacgag attttatcg 
aaaatttca tcaattaag gctgtgcgt aatgacggt 
ttcccgtct ttaatttgc accaaatgt atcgatttg 
gcacacact tcgttcaat ttccaaaaa ctgtgcaac 
cggtcgcaa aatatcctc gaaacgttt gttaaacaa 
caaccggag acgagtatt aagcttgag aatgttgag 
aaaaatgag tttctggtc gttgaacga gcgtctgat 
gaagagtcg gataaagcg gacgtgcca gggcaggca 
aatgaggag gaaaaaaat tttgcgtgc acaatttgc 
gagaaaagc tacaaattt gcgaacagt ttgcgcgtc 
catatgcgc aaccactcg gaggagaag ccctacgag 
tgtaaaacg tgcggtaaa tgttttaaa cagtacggt 
tcgcttgtc taccaccag cggagtcac acaggtgtg 
cagccttac gtgtgcaaa acttgtggt aaaaagtac 
aaacaatcg gggactctg acggcccac atgagggtg 
catacgggg cagaggccc ttcctgtgc tcgatctgt 
ggcaggggg tttagacaa gcggccgat ttggggtac 
catatgagg tcgcacacg aaagaaaag ccctacatg 
tgcaacatt tgcggtaag acgatgtca atgcagtcg 
cacttggtg cagcatttg cggacgcac acgggggaa 
agaccgtac aaatgcgat gtgtgtggg aaggcgttt 
ccgtcgtcg actcggctc aaaagacat gcgattatc 
catactagt ctcaagccg tacacttgt gaggtttgt 
gcgaaaagt ttcaacagg atgagtagt ttgaaggtt 
catgttaaa gtgcatacg gacgaaagg tcgcatgtt 
tgtcccatt tgtagcaaa gggtttata caagcgcag 
gcactaagg tcgcatttg gggtcgcat tcgtccgaa 
ctaagcgtt aaaccagct cagggtttt taa
    mRNA sequence
    • >TC011010 RA
      gtgattggt tgattcgga gccgtcaag tgtcaaaca 
actgtcact ttgacaacg tcaataata aacaattta 
tttgactta aaaaatgga aaatgtgcg gacgcttga 
cttggataa gatctgtag aacgtgtct gactgaaaa 
taaggacat gttttcgat ttttagcca attgtacga 
agtgcactc acaggagag tgatattcc gtgtattga 
cgagatttt atcgaaaat ttcatcaat taaggctgt 
gcgtaatga cggtttccc gtctttaat ttgcaccaa 
atgtatcga tttggcaca cacttcgtt caatttcca 
aaaactgtg caaccggtc gcaaaatat cctcgaaac 
gtttgttaa acaacaacc ggagacgag tattaagct 
tgagaatgt tgagaaaaa tgagtttct ggtcgttga 
acgagcgtc tgatgaaga gtcggataa agcggacgt 
gccagggca ggcaaatga ggaggaaaa aaattttgc 
gtgcacaat ttgcgagaa aagctacaa atttgcgaa 
cagtttgcg cgtccatat gcgcaacca ctcggagga 
gaagcccta cgagtgtaa aacgtgcgg taaatgttt 
taaacagta cggttcgct tgtctacca ccagcggag 
tcacacagg tgtgcagcc ttacgtgtg caaaacttg 
tggtaaaaa gtacaaaca atcggggac tctgacggc 
ccacatgag ggtgcatac ggggcagag gcccttcct 
gtgctcgat ctgtggcag ggggtttag acaagcggc 
cgatttggg gtaccatat gaggtcgca cacgaaaga 
aaagcccta catgtgcaa catttgcgg taagacgat 
gtcaatgca gtcgcactt ggtgcagca tttgcggac 
gcacacggg ggaaagacc gtacaaatg cgatgtgtg 
tgggaaggc gtttccgtc gtcgactcg gctcaaaag 
acatgcgat tatccatac tagtctcaa gccgtacac 
ttgtgaggt ttgtgcgaa aagtttcaa caggatgag 
tagtttgaa ggttcatgt taaagtgca tacggacga 
aaggtcgca tgtttgtcc catttgtag caaagggtt 
tatacaagc gcaggcact aaggtcgca tttggggtc 
gcattcgtc cgaactaag cgttaaacc agctcaggg 
tttttaata aaaaatatt tgtgaagag ttgcatttt 
tttcatctt gtcctttat ttaaacaaa taattattg 
ttttctgtg atttgtcat aagttcgtc caattcttg 
gccacgttc tgcttttgc gggttttgg tagaaatcg 
gtgaataac aggactgac caacatttt atcaactgc 
tctgttctc tctgtgtgt gaaataatt tataattag 
ggcaacaat tatcgtcaa aattgttcc aaatacaca 
ttgataatt gta
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