Status
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Gene information
General information
TC010995 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450827.1: 47207..57743
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010995
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04960

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010995 RA
      MPNSCQNFL TQEKNHEAS WFERTAKLI REKFKSKNK 
      DFEDDLETE QEYKERWRS IYIIYFTMF LISLGFSII 
      VTGVWPYLD KLDPTAGKE FMGYIVAAN PFGQMLFSP 
      LVGWWSNRL GSIRLPLLC SLALFTTSS AIYSSLELF 
      NDYRKHWML WTRFLVGVS SANIAACRS YLSAATRVS 
      ERTTAVSMI SLAQVLGFI VGPAIQAAV VPLGDDGFW 
      IIPNRLRLN MYTASGWIN VIFSLFNMY LFLPHVFKE 
      HKIAGREAM LKQGKDNER DTWKQHRPN YFATWTLIV 
      AFFVIVFNF MLLETLGTP VTMDQFAWS KAASLKYMG 
      ILMSVGAIL AIVTFASIS PLCKRFSEV KVMIWGGFL 
      FMVLGRAIY IPFNNDPPQ MYDSDLKLN LTLVCDKLL 
      FNRTVSNYE TMWSINSTD ETTVRNLTL NCGEDLVGC 
      PSSQEWCTY TPAMTLTQF IIGYFLTAF GYPIGVTLI 
      QTIFSKLLG PRPQGVWMG LMTGSGCLS RVMGPVFVT 
      YIYEEFGTI WTFGLTTVM MVICLFWLL YFNKTLIPV 
      EIQTVPEGE ELKDINDLV TIPLNDKIQ DD
    CDS sequence
    • >TC010995 RA
      atgccaaac agttgtcaa aacttcctt acacaggag 
      aaaaaccat gaggcatct tggtttgaa agaacagct 
      aagctcatc cgcgaaaaa tttaaatcg aagaacaaa 
      gactttgag gatgatcta gaaaccgaa caagaatat 
      aaggaacga tggagatca atttacatc atttatttc 
      acaatgttt ttaatttct ctggggttt agtattata 
      gtcactgga gtatggcct tatttggac aaactagac 
      ccaactgcc ggaaaagaa tttatgggt tacatcgtt 
      gcagcgaac ccttttggc caaatgctc ttctctcca 
      ctggtcggt tggtggagc aatagattg ggttcaatt 
      cgtttacca ttactttgt tcattggca ctttttacg 
      acatccagt gcaatttac tcatcgctg gaattgttt 
      aacgactat aggaagcac tggatgttg tggaccagg 
      tttttggtg ggagttagt tcagctaat atagcggct 
      tgtcgctcg tacctttcc gcagctaca agagtctcc 
      gaacgaacc acagccgtt tcaatgata tccttagcc 
      caagtattg ggtttcatt gtaggtcca gcaatccaa 
      gctgccgtt gtcccatta ggagacgat ggattttgg 
      ataattcca aaccgactt agactgaac atgtacaca 
      gcttccggc tggataaat gtaattttt tccctattc 
      aacatgtac cttttcctt ccacacgta ttcaaagaa 
      cacaaaatc gccggcaga gaagcgatg ctaaaacaa 
      ggcaaagac aacgaacga gatacgtgg aaacaacac 
      cgcccgaat tattttgcc acttggact ttaattgtt 
      gcgtttttt gtcatcgtt ttcaacttt atgctactt 
      gaaacactg ggaacacca gtgaccatg gaccaattt 
      gcttggtcc aaagcagca tcgcttaaa tacatgggg 
      attttaatg tcagtgggc gctattttg gcaatagtt 
      acgtttgcc tcaatttca cctctgtgt aagagattt 
      tccgaagtg aaagtgatg atttggggt gggtttttg 
      tttatggtt ttgggcaga gcgatttat attcccttt 
      aataatgac ccacctcaa atgtacgat tctgatttg 
      aagctgaat ctgaccctc gtctgtgat aaattgctc 
      tttaatcga actgtgtcg aattatgaa actatgtgg 
      tcaataaat tcaacggat gaaacaact gttcgaaat 
      ttgactctg aattgtggt gaagattta gttggatgt 
      ccttcgagt caggaatgg tgcacttat acgcctgca 
      atgaccctc actcagttt attattggg tatttcctg 
      acggctttt ggttatccg attggggtt acgctgatt 
      caaacgatc ttctcgaag ttgctagga ccacgacct 
      cagggagtt tggatgggt ttaatgact ggttctggt 
      tgtttgtca cgtgtcatg ggtccagta tttgtgaca 
      tacatctat gaggaattt ggtacaatt tggacgttt 
      ggcctaact acagtcatg atggttatt tgtctattt 
      tggcttctt tattttaac aagacttta attcctgtt 
      gagattcag actgtacct gaaggtgaa gaattgaag 
      gacattaat gatttggtt accattcct ttaaatgat 
      aaaattcaa gacgattga
    mRNA sequence
    • >TC010995 RA
      gtggttttt gaccggcta tctctattg acacaattt 
      tcacacatg cgcagtaga gggaggtgg aggtggttt 
      ttgaaccgg ctatctcta tctactact cagcaaggc 
      ttaaaattg ctttaaatt tgttaaaaa taatctgac 
      gtttcgtac tgagaaatg ccaaacagt tgtcaaaac 
      ttccttaca caggagaaa aaccatgag gcatcttgg 
      tttgaaaga acagctaag ctcatccgc gaaaaattt 
      aaatcgaag aacaaagac tttgaggat gatctagaa 
      accgaacaa gaatataag gaacgatgg agatcaatt 
      tacatcatt tatttcaca atgttttta atttctctg 
      gggtttagt attatagtc actggagta tggccttat 
      ttggacaaa ctagaccca actgccgga aaagaattt 
      atgggttac atcgttgca gcgaaccct tttggccaa 
      atgctcttc tctccactg gtcggttgg tggagcaat 
      agattgggt tcaattcgt ttaccatta ctttgttca 
      ttggcactt tttacgaca tccagtgca atttactca 
      tcgctggaa ttgtttaac gactatagg aagcactgg 
      atgttgtgg accaggttt ttggtggga gttagttca 
      gctaatata gcggcttgt cgctcgtac ctttccgca 
      gctacaaga gtctccgaa cgaaccaca gccgtttca 
      atgatatcc ttagcccaa gtattgggt ttcattgta 
      ggtccagca atccaagct gccgttgtc ccattagga 
      gacgatgga ttttggata attccaaac cgacttaga 
      ctgaacatg tacacagct tccggctgg ataaatgta 
      attttttcc ctattcaac atgtacctt ttccttcca 
      cacgtattc aaagaacac aaaatcgcc ggcagagaa 
      gcgatgcta aaacaaggc aaagacaac gaacgagat 
      acgtggaaa caacaccgc ccgaattat tttgccact 
      tggacttta attgttgcg ttttttgtc atcgttttc 
      aactttatg ctacttgaa acactggga acaccagtg 
      accatggac caatttgct tggtccaaa gcagcatcg 
      cttaaatac atggggatt ttaatgtca gtgggcgct 
      attttggca atagttacg tttgcctca atttcacct 
      ctgtgtaag agattttcc gaagtgaaa gtgatgatt 
      tggggtggg tttttgttt atggttttg ggcagagcg 
      atttatatt ccctttaat aatgaccca cctcaaatg 
      tacgattct gatttgaag ctgaatctg accctcgtc 
      tgtgataaa ttgctcttt aatcgaact gtgtcgaat 
      tatgaaact atgtggtca ataaattca acggatgaa 
      acaactgtt cgaaatttg actctgaat tgtggtgaa 
      gatttagtt ggatgtcct tcgagtcag gaatggtgc 
      acttatacg cctgcaatg accctcact cagtttatt 
      attgggtat ttcctgacg gcttttggt tatccgatt 
      ggggttacg ctgattcaa acgatcttc tcgaagttg 
      ctaggacca cgacctcag ggagtttgg atgggttta 
      atgactggt tctggttgt ttgtcacgt gtcatgggt 
      ccagtattt gtgacatac atctatgag gaatttggt 
      acaatttgg acgtttggc ctaactaca gtcatgatg 
      gttatttgt ctattttgg cttctttat tttaacaag 
      actttaatt cctgttgag attcagact gtacctgaa 
      ggtgaagaa ttgaaggac attaatgat ttggttacc 
      attccttta aatgataaa attcaagac gattgagtt 
      tttgaattt ctgggagag gtaaaatgt tgttactta 
      cgtactaga ttgactcag ccaaacatt gagggtttt 
      tactgatgg atctcaaga ctgtatcac ataccagat 
      atttaaata ttgtaaaac tataagtga tatttttac 
      tagttaacg tacttaaga gtatatgtt atttttata 
      ttttgtgag atgaaataa aatgccaat caaata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY