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Gene information
General information
TC010989 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3059568..3069151
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010989
    iBeetle screen iB_04958

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010989 RA
      MSGTVCSVA VCKSNFRRA KREGELIRF FTFPKNPEI 
      CKEWERRCY RKDPWSSKN KRICSKHFT DKDYQDRLQ 
      AQLLNLRPQ KLKPTAVPS LHLLPETTS HLELSKSNE 
      KKLVKKNRK QIFDKVPVR EDKPIPESA SVEIEEDFS 
      NNSECEVDI LKNKMNILV KENGKLKKL LEEKNQQVT 
      LLQQKLNEL ESLISRKNV SNFEENTSP NQLDIVLKK 
      IKNTQKVIE GLTKSQGVR SSSRKRKLT NKK
    CDS sequence
    • >TC010989 RA
      atgagtgga actgtatgc agtgtagca gtttgcaaa 
      agcaacttt agaagagcc aaaagagag ggcgaatta 
      attaggttt ttcactttt cctaaaaat ccagaaatt 
      tgcaaagaa tgggaaagg agatgttat cgaaaagat 
      ccgtggagt tcgaagaat aaaaggata tgtagcaaa 
      cacttcact gataaggat tatcaggac cgactgcaa 
      gcacaacta ttaaattta cgaccacag aagttaaaa 
      cctactgct gttccgtca ttgcatctc ttgccagaa 
      actacatct catttagaa cttagtaaa agcaacgag 
      aaaaagctc gttaagaaa aatcgtaaa caaattttt 
      gataaagtg ccggtacgt gaagataaa cctattcca 
      gaatctgcg agtgttgaa attgaagag gatttctcc 
      aacaattct gaatgtgaa gtggatatt ttaaaaaat 
      aaaatgaat attttggtt aaagagaat ggaaaacta 
      aagaaatta ttagaagaa aagaaccaa caagttaca 
      ctgctgcaa caaaaactt aacgaatta gagagtctt 
      attagtcga aaaaatgtg tctaatttt gaagaaaac 
      acatcacca aatcagtta gacattgtt ttgaaaaaa 
      attaaaaat acgcagaaa gttatagaa ggactaaca 
      aaaagtcaa ggtgttaga agttccagc aggaaacga 
      aaattaacc aacaaaaag tga
    mRNA sequence
    • >TC010989 RA
      ccactgatg tttgttttg agtcagtcg cggacgtaa 
      tgcgtagat aattatttg aattttcgt taatttttt 
      ttaaaattg tgttgtaaa ctcttagtc cgtgtctta 
      gttttcatt ttattgtcg taggttttc taaaaatgc 
      atcaacagc tcggaaata caacacatt ttctctatg 
      atatacacc catggttag tacaaagtg gttgagcgg 
      taaatgcct gaatagagg ggataaccg ccatattga 
      tacgccatc agtatgcca acgtcaatc aacgatcaa 
      cgtgtttag cagtgttaa gaatgctaa tgttacagc 
      ttagagatg agtggaact gtatgcagt gtagcagtt 
      tgcaaaagc aactttaga agagccaaa agagagggc 
      gaattaatt aggtttttc acttttcct aaaaatcca 
      gaaatttgc aaagaatgg gaaaggaga tgttatcga 
      aaagatccg tggagttcg aagaataaa aggatatgt 
      agcaaacac ttcactgat aaggattat caggaccga 
      ctgcaagca caactatta aatttacga ccacagaag 
      ttaaaacct actgctgtt ccgtcattg catctcttg 
      ccagaaact acatctcat ttagaactt agtaaaagc 
      aacgagaaa aagctcgtt aagaaaaat cgtaaacaa 
      atttttgat aaagtgccg gtacgtgaa gataaacct 
      attccagaa tctgcgagt gttgaaatt gaagaggat 
      ttctccaac aattctgaa tgtgaagtg gatatttta 
      aaaaataaa atgaatatt ttggttaaa gagaatgga 
      aaactaaag aaattatta gaagaaaag aaccaacaa 
      gttacactg ctgcaacaa aaacttaac gaattagag 
      agtcttatt agtcgaaaa aatgtgtct aattttgaa 
      gaaaacaca tcaccaaat cagttagac attgttttg 
      aaaaaaatt aaaaatacg cagaaagtt atagaagga 
      ctaacaaaa agtcaaggt gttagaagt tccagcagg 
      aaacgaaaa ttaaccaac aaaaagtga aaaactcca 
      taatcagtt tataatata tatatattt ttctt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY