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Gene information
General information
TC010974 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3052971..3059466
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010974
    iBeetle screen iB_04957

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    antenna distal swollen - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    legs segment swollen - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Adult morphology
    elytra not closed - Number of animals showing the phenotype: 2


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010974 RA
      MINEEGRRF LEELKSGKV LEKPYLMNF FFILSFSDL 
      KKFHFYYWF AYPVPHNLE ISVEGISPI SEHFTEAEV 
      ASLSTEYTR LDPHQKPYF LIHNKKLHT YHSKLSQVS 
      ENNCQEYYF AFADYINSG QHPKSQIKN YIIFLLHHC 
      PFLGGKTLN FLLFFLDRK LSCCQSLIY KLTLPQCNS 
      TIDDIIYGG GDNAWAGWE KNEKNKFAP RFSNMRTTM 
      DPQILFEES VDLNLKLMK WRLLPDINL DKIKNAKCL 
      LLGAGTLGC SVARNLLGW GVRNINFVD NSTVSYSNP 
      VRQHLFTYE DAVKSKPKA EAAAESLHK IFPSINSQG 
      HQFTIPMPG HNVGESTVE SVKKSVEDL EKLIQEHDI 
      VFLLTDSRE SRWLPTLLG IFHNKIVIN VALGFDTYL 
      IMRYGRKDI EDNVKEVQT HSAFKRISG NELGCYFCN 
      DVTAPGNSL KDRTLDQQC TVTRPGVSS IAGALSVEL 
      TVSLLQHEE GINAPAFYK TGPQHEMNF TDDQGGVLG 
      ILPHSIRGF LSSFMHVLP ATPKYNQCI ACSSIVLEE 
      YKAKGFAFL LETFNSNNY LENLTGLSK LFADSNYAD 
      VLELSDEEW EN
    • >TC010974 RB
      MSSTKPLLQ LVTVSSFVQ PSFWNKLSE LKINVDKLN 
      DDERQIYGF FSNSPTTWT THIVEVDST SFNTTLNSQ 
      NNIPFQGKI FNKNTIEQF KDCDKTKMI NEEGRRFLE 
      ELKSGKVLE KPYLMNFFF ILSFSDLKK FHFYYWFAY 
      PVPHNLEIS VEGISPISE HFTEAEVAS LSTEYTRLD 
      PHQKPYFLI HNKKLHTYH SKLSQVSEN NCQEYYFAF 
      ADYINSGQH PKSQIKNYI IFLLHHCPF LGGKTLNFL 
      LFFLDRKLS CCQSLIYKL TLPQCNSTI DDIIYGGGD 
      NAWAGWEKN EKNKFAPRF SNMRTTMDP QILFEESVD 
      LNLKLMKWR LLPDINLDK IKNAKCLLL GAGTLGCSV 
      ARNLLGWGV RNINFVDNS TVSYSNPVR QHLFTYEDA 
      VKSKPKAEA AAESLHKIF PSINSQGHQ FTIPMPGHN 
      VGESTVESV KKSVEDLEK LIQEHDIVF LLTDSRESR 
      WLPTLLGIF HNKIVINVA LGFDTYLIM RYGRKDIED 
      NVKEVQTHS AFKRISGNE LGCYFCNDV TAPGNSLKD 
      RTLDQQCTV TRPGVSSIA GALSVELTV SLLQHEEGI 
      NAPAFYKTG PQHEMNFTD DQGGVLGIL PHSIRGFLS 
      SFMHVLPAT PKYNQCIAC SSIVLEEYK AKGFAFLLE 
      TFNSNNYLE NLTGLSKLF ADSNYADVL ELSDEEWEN
    CDS sequence
    • >TC010974 RA
      atgattaac gaagaaggt agacggttt ttggaagag 
      ttgaaaagt ggaaaggtt ttggagaag ccctatctg 
      atgaacttc ttttttatt ttgtcattc agcgatctt 
      aaaaagttc catttttat tattggttt gcataccca 
      gttccccat aatctggaa atttcagta gaaggaatt 
      agtcctatt tcagagcat tttacagag gctgaagtg 
      gcgtcttta agtactgaa tatacccga ctagatcct 
      caccaaaag ccatacttt ttaatccat aataaaaaa 
      ctccatact taccactcg aaactgagc caagtcagt 
      gagaataat tgccaggag tattatttt gcttttgca 
      gattacatc aacagtggc caacatccg aaatctcaa 
      ataaagaac tacattatt ttcttgtta catcattgc 
      cctttcctt ggaggtaaa actttaaat tttctgttg 
      ttcttcttg gaccgtaaa ttatcctgc tgtcaaagt 
      ttaatctat aaattaact ctacctcaa tgcaactca 
      acaatcgat gatattatt tatgggggt ggcgacaac 
      gcgtgggct ggatgggaa aaaaatgaa aaaaataaa 
      tttgcacca cgattttct aacatgcga acaacgatg 
      gatcctcaa atattattt gaagaaagt gttgatcta 
      aatttgaaa ctgatgaag tggcgtctt ctccctgat 
      attaattta gataaaata aagaacgca aaatgcctt 
      cttctaggc gctggaact ttgggatgt tcagtagca 
      agaaatctt cttgggtgg ggtgttaga aacatcaac 
      tttgtggac aattcaacg gtttcttac tcaaatcca 
      gttagacaa cacctgttc acttatgaa gacgcagtt 
      aaaagtaaa ccaaaggct gaagctgcg gctgaaagt 
      ttgcacaag atatttccc tcaataaat tctcaaggt 
      catcaattt acgatccca atgcctggg cacaatgtt 
      ggtgaaagt actgttgaa tctgtgaag aaaagtgta 
      gaggattta gaaaaactc attcaagaa cacgatatt 
      gttttcctt ttgactgac tccagagag agccgctgg 
      ttgcctaca ttactcggc atattccat aataaaata 
      gtaataaat gttgctttg ggcttcgat acttacttg 
      atcatgaga tatggccgc aaagatatt gaagataat 
      gttaaagaa gtacaaact cattcggca tttaaaaga 
      atatccgga aacgaactc ggatgttat ttttgcaat 
      gatgttact gctccagga aactctcta aaggaccga 
      actcttgac cagcaatgc accgtcacc agacctgga 
      gtctcctca atcgcagga gctttgtct gttgagtta 
      accgtttca ctgcttcaa catgaagaa ggcattaac 
      gcacctgca ttttacaaa actggtccc cagcacgaa 
      atgaatttt accgacgac caagggggc gttttagga 
      atattacct cactcgatt agaggattc ttatcaagt 
      tttatgcac gttttacct gccacacca aagtataat 
      caatgcatt gcttgctct agtattgtt ctagaagag 
      tacaaggcc aaaggtttt gcttttctt ctcgaaaca 
      ttcaacagc aacaattat ctggagaat ttaactgga 
      ctcagcaaa ttgtttgct gacagcaat tatgctgat 
      gtattggaa ttaagtgat gaagaatgg gagaattaa
    • >TC010974 RB
      atgtcctca acgaagccc cttctgcaa cttgtgaca 
      gtttcatca tttgtgcaa ccgagtttt tggaacaaa 
      ttatccgaa ttaaaaatt aatgttgac aaattaaac 
      gatgatgaa cgccaaatc tatggtttc ttctcaaac 
      agccctaca acgtggacc acacatatt gtggaagtg 
      gacagcaca tcatttaac acaacgctt aattcacaa 
      aacaacatt ccatttcaa ggaaaaata ttcaataaa 
      aacacaatt gaacagttt aaagattgt gacaaaacc 
      aaaatgatt aacgaagaa ggtagacgg tttttggaa 
      gagttgaaa agtggaaag gttttggag aagccctat 
      ctgatgaac ttctttttt attttgtca ttcagcgat 
      cttaaaaag ttccatttt tattattgg tttgcatac 
      ccagttccc cataatctg gaaatttca gtagaagga 
      attagtcct atttcagag cattttaca gaggctgaa 
      gtggcgtct ttaagtact gaatatacc cgactagat 
      cctcaccaa aagccatac tttttaatc cataataaa 
      aaactccat acttaccac tcgaaactg agccaagtc 
      agtgagaat aattgccag gagtattat tttgctttt 
      gcagattac atcaacagt ggccaacat ccgaaatct 
      caaataaag aactacatt attttcttg ttacatcat 
      tgccctttc cttggaggt aaaacttta aattttctg 
      ttgttcttc ttggaccgt aaattatcc tgctgtcaa 
      agtttaatc tataaatta actctacct caatgcaac 
      tcaacaatc gatgatatt atttatggg ggtggcgac 
      aacgcgtgg gctggatgg gaaaaaaat gaaaaaaat 
      aaatttgca ccacgattt tctaacatg cgaacaacg 
      atggatcct caaatatta tttgaagaa agtgttgat 
      ctaaatttg aaactgatg aagtggcgt cttctccct 
      gatattaat ttagataaa ataaagaac gcaaaatgc 
      cttcttcta ggcgctgga actttggga tgttcagta 
      gcaagaaat cttcttggg tggggtgtt agaaacatc 
      aactttgtg gacaattca acggtttct tactcaaat 
      ccagttaga caacacctg ttcacttat gaagacgca 
      gttaaaagt aaaccaaag gctgaagct gcggctgaa 
      agtttgcac aagatattt ccctcaata aattctcaa 
      ggtcatcaa tttacgatc ccaatgcct gggcacaat 
      gttggtgaa agtactgtt gaatctgtg aagaaaagt 
      gtagaggat ttagaaaaa ctcattcaa gaacacgat 
      attgttttc cttttgact gactccaga gagagccgc 
      tggttgcct acattactc ggcatattc cataataaa 
      atagtaata aatgttgct ttgggcttc gatacttac 
      ttgatcatg agatatggc cgcaaagat attgaagat 
      aatgttaaa gaagtacaa actcattcg gcatttaaa 
      agaatatcc ggaaacgaa ctcggatgt tatttttgc 
      aatgatgtt actgctcca ggaaactct ctaaaggac 
      cgaactctt gaccagcaa tgcaccgtc accagacct 
      ggagtctcc tcaatcgca ggagctttg tctgttgag 
      ttaaccgtt tcactgctt caacatgaa gaaggcatt 
      aacgcacct gcattttac aaaactggt ccccagcac 
      gaaatgaat tttaccgac gaccaaggg ggcgtttta 
      ggaatatta cctcactcg attagagga ttcttatca 
      agttttatg cacgtttta cctgccaca ccaaagtat 
      aatcaatgc attgcttgc tctagtatt gttctagaa 
      gagtacaag gccaaaggt tttgctttt cttctcgaa 
      acattcaac agcaacaat tatctggag aatttaact 
      ggactcagc aaattgttt gctgacagc aattatgct 
      gatgtattg gaattaagt gatgaagaa tgggagaat 
      taa
    mRNA sequence
    • >TC010974 RA
      acaacaaaa atgtgaggt taggttttc agttctgtc 
      agtatcagc tgttcttaa aaaattcaa aaaatgtcc 
      tcaacgaag ccccttctg caacttgtg acagtttca 
      tcatttgtg caaccgagt ttttggaac aaattatcc 
      gaattaaaa attaatgtt gacaaatta aacgatgat 
      gaacgccaa atctatggt ttcttctca aacagccct 
      acaacgtgg accacacat attgtggaa gtggacagc 
      acatcattt aacacgtaa ttaataatg aaatttaca 
      tgtatttcg ctcataatg tgttataaa ttctagaac 
      gcttaattc acaaaacaa cattccatt tcaaggaaa 
      aatattcaa taaaaacac aattgaaca gtttaaaga 
      ttgtgacaa aaccaaaat gattaacga agaaggtag 
      acggttttt ggaagagtt gaaaagtgg aaaggtttt 
      ggagaagcc ctatctgat gaacttctt ttttatttt 
      gtcattcag cgatcttaa aaagttcca tttttatta 
      ttggtttgc atacccagt tccccataa tctggaaat 
      ttcagtaga aggaattag tcctatttc agagcattt 
      tacagaggc tgaagtggc gtctttaag tactgaata 
      tacccgact agatcctca ccaaaagcc atacttttt 
      aatccataa taaaaaact ccatactta ccactcgaa 
      actgagcca agtcagtga gaataattg ccaggagta 
      ttattttgc ttttgcaga ttacatcaa cagtggcca 
      acatccgaa atctcaaat aaagaacta cattatttt 
      cttgttaca tcattgccc tttccttgg aggtaaaac 
      tttaaattt tctgttgtt cttcttgga ccgtaaatt 
      atcctgctg tcaaagttt aatctataa attaactct 
      acctcaatg caactcaac aatcgatga tattattta 
      tgggggtgg cgacaacgc gtgggctgg atgggaaaa 
      aaatgaaaa aaataaatt tgcaccacg attttctaa 
      catgcgaac aacgatgga tcctcaaat attatttga 
      agaaagtgt tgatctaaa tttgaaact gatgaagtg 
      gcgtcttct ccctgatat taatttaga taaaataaa 
      gaacgcaaa atgccttct tctaggcgc tggaacttt 
      gggatgttc agtagcaag aaatcttct tgggtgggg 
      tgttagaaa catcaactt tgtggacaa ttcaacggt 
      ttcttactc aaatccagt tagacaaca cctgttcac 
      ttatgaaga cgcagttaa aagtaaacc aaaggctga 
      agctgcggc tgaaagttt gcacaagat atttccctc 
      aataaattc tcaaggtca tcaatttac gatcccaat 
      gcctgggca caatgttgg tgaaagtac tgttgaatc 
      tgtgaagaa aagtgtaga ggatttaga aaaactcat 
      tcaagaaca cgatattgt tttcctttt gactgactc 
      cagagagag ccgctggtt gcctacatt actcggcat 
      attccataa taaaatagt aataaatgt tgctttggg 
      cttcgatac ttacttgat catgagata tggccgcaa 
      agatattga agataatgt taaagaagt acaaactca 
      ttcggcatt taaaagaat atccggaaa cgaactcgg 
      atgttattt ttgcaatga tgttactgc tccaggaaa 
      ctctctaaa ggaccgaac tcttgacca gcaatgcac 
      cgtcaccag acctggagt ctcctcaat cgcaggagc 
      tttgtctgt tgagttaac cgtttcact gcttcaaca 
      tgaagaagg cattaacgc acctgcatt ttacaaaac 
      tggtcccca gcacgaaat gaattttac cgacgacca 
      agggggcgt tttaggaat attacctca ctcgattag 
      aggattctt atcaagttt tatgcacgt tttacctgc 
      cacaccaaa gtataatca atgcattgc ttgctctag 
      tattgttct agaagagta caaggccaa aggttttgc 
      ttttcttct cgaaacatt caacagcaa caattatct 
      ggagaattt aactggact cagcaaatt gtttgctga 
      cagcaatta tgctgatgt attggaatt aagtgatga 
      agaatggga gaattaact tacaaagaa gtttcggag 
      aaacgcctt aggccgcct ttgcttacg ttatttaag 
      tactagcaa gcatttttt atgtacaaa cattatatg 
      aatgtattt attacaatt taagaaata aatctcaat 
      cgt
    • >TC010974 RB
      tctctctta ttctatgca caacaacaa aaatgtgag 
      gttaggttt tcagttctg tcagtatca gctgttctt 
      aaaaaattc aaaaaatgt cctcaacga agccccttc 
      tgcaacttg tgacagttt catcatttg tgcaaccga 
      gtttttgga acaaattat ccgaattaa aaattaatg 
      ttgacaaat taaacgatg atgaacgcc aaatctatg 
      gtttcttct caaacagcc ctacaacgt ggaccacac 
      atattgtgg aagtggaca gcacatcat ttaacacaa 
      cgcttaatt cacaaaaca acattccat ttcaaggaa 
      aaatattca ataaaaaca caattgaac agtttaaag 
      attgtgaca aaaccaaaa tgattaacg aagaaggta 
      gacggtttt tggaagagt tgaaaagtg gaaaggttt 
      tggagaagc cctatctga tgaacttct tttttattt 
      tgtcattca gcgatctta aaaagttcc atttttatt 
      attggtttg catacccag ttccccata atctggaaa 
      tttcagtag aaggaatta gtcctattt cagagcatt 
      ttacagagg ctgaagtgg cgtctttaa gtactgaat 
      atacccgac tagatcctc accaaaagc catactttt 
      taatccata ataaaaaac tccatactt accactcga 
      aactgagcc aagtcagtg agaataatt gccaggagt 
      attattttg cttttgcag attacatca acagtggcc 
      aacatccga aatctcaaa taaagaact acattattt 
      tcttgttac atcattgcc ctttccttg gaggtaaaa 
      ctttaaatt ttctgttgt tcttcttgg accgtaaat 
      tatcctgct gtcaaagtt taatctata aattaactc 
      tacctcaat gcaactcaa caatcgatg atattattt 
      atgggggtg gcgacaacg cgtgggctg gatgggaaa 
      aaaatgaaa aaaataaat ttgcaccac gattttcta 
      acatgcgaa caacgatgg atcctcaaa tattatttg 
      aagaaagtg ttgatctaa atttgaaac tgatgaagt 
      ggcgtcttc tccctgata ttaatttag ataaaataa 
      agaacgcaa aatgccttc ttctaggcg ctggaactt 
      tgggatgtt cagtagcaa gaaatcttc ttgggtggg 
      gtgttagaa acatcaact ttgtggaca attcaacgg 
      tttcttact caaatccag ttagacaac acctgttca 
      cttatgaag acgcagtta aaagtaaac caaaggctg 
      aagctgcgg ctgaaagtt tgcacaaga tatttccct 
      caataaatt ctcaaggtc atcaattta cgatcccaa 
      tgcctgggc acaatgttg gtgaaagta ctgttgaat 
      ctgtgaaga aaagtgtag aggatttag aaaaactca 
      ttcaagaac acgatattg ttttccttt tgactgact 
      ccagagaga gccgctggt tgcctacat tactcggca 
      tattccata ataaaatag taataaatg ttgctttgg 
      gcttcgata cttacttga tcatgagat atggccgca 
      aagatattg aagataatg ttaaagaag tacaaactc 
      attcggcat ttaaaagaa tatccggaa acgaactcg 
      gatgttatt tttgcaatg atgttactg ctccaggaa 
      actctctaa aggaccgaa ctcttgacc agcaatgca 
      ccgtcacca gacctggag tctcctcaa tcgcaggag 
      ctttgtctg ttgagttaa ccgtttcac tgcttcaac 
      atgaagaag gcattaacg cacctgcat tttacaaaa 
      ctggtcccc agcacgaaa tgaatttta ccgacgacc 
      aagggggcg ttttaggaa tattacctc actcgatta 
      gaggattct tatcaagtt ttatgcacg ttttacctg 
      ccacaccaa agtataatc aatgcattg cttgctcta 
      gtattgttc tagaagagt acaaggcca aaggttttg 
      cttttcttc tcgaaacat tcaacagca acaattatc 
      tggagaatt taactggac tcagcaaat tgtttgctg 
      acagcaatt atgctgatg tattggaat taagtgatg 
      aagaatggg agaattaac ttacaaaga agtttcgga 
      gaaacgcct taggccgcc tttgcttac gttatttaa 
      gtactagca agcattttt tatgtacaa acattatat 
      gaatgtatt tattacaat ttaagaaat aaatctcaa 
      tcgt
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