Status
Please wait ...
Gene information
General information
TC010974 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3052971..3059466
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC010974
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04957

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     antenna distal swollen - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    legs segment swollen - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection

    Adult morphology
    elytra not closed - Number of animals showing the phenotype: 2


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010974 RA
      MINEEGRRF LEELKSGKV LEKPYLMNF FFILSFSDL 
KKFHFYYWF AYPVPHNLE ISVEGISPI SEHFTEAEV 
ASLSTEYTR LDPHQKPYF LIHNKKLHT YHSKLSQVS 
ENNCQEYYF AFADYINSG QHPKSQIKN YIIFLLHHC 
PFLGGKTLN FLLFFLDRK LSCCQSLIY KLTLPQCNS 
TIDDIIYGG GDNAWAGWE KNEKNKFAP RFSNMRTTM 
DPQILFEES VDLNLKLMK WRLLPDINL DKIKNAKCL 
LLGAGTLGC SVARNLLGW GVRNINFVD NSTVSYSNP 
VRQHLFTYE DAVKSKPKA EAAAESLHK IFPSINSQG 
HQFTIPMPG HNVGESTVE SVKKSVEDL EKLIQEHDI 
VFLLTDSRE SRWLPTLLG IFHNKIVIN VALGFDTYL 
IMRYGRKDI EDNVKEVQT HSAFKRISG NELGCYFCN 
DVTAPGNSL KDRTLDQQC TVTRPGVSS IAGALSVEL 
TVSLLQHEE GINAPAFYK TGPQHEMNF TDDQGGVLG 
ILPHSIRGF LSSFMHVLP ATPKYNQCI ACSSIVLEE 
YKAKGFAFL LETFNSNNY LENLTGLSK LFADSNYAD 
VLELSDEEW EN
    • >TC010974 RB
      MSSTKPLLQ LVTVSSFVQ PSFWNKLSE LKINVDKLN 
DDERQIYGF FSNSPTTWT THIVEVDST SFNTTLNSQ 
NNIPFQGKI FNKNTIEQF KDCDKTKMI NEEGRRFLE 
ELKSGKVLE KPYLMNFFF ILSFSDLKK FHFYYWFAY 
PVPHNLEIS VEGISPISE HFTEAEVAS LSTEYTRLD 
PHQKPYFLI HNKKLHTYH SKLSQVSEN NCQEYYFAF 
ADYINSGQH PKSQIKNYI IFLLHHCPF LGGKTLNFL 
LFFLDRKLS CCQSLIYKL TLPQCNSTI DDIIYGGGD 
NAWAGWEKN EKNKFAPRF SNMRTTMDP QILFEESVD 
LNLKLMKWR LLPDINLDK IKNAKCLLL GAGTLGCSV 
ARNLLGWGV RNINFVDNS TVSYSNPVR QHLFTYEDA 
VKSKPKAEA AAESLHKIF PSINSQGHQ FTIPMPGHN 
VGESTVESV KKSVEDLEK LIQEHDIVF LLTDSRESR 
WLPTLLGIF HNKIVINVA LGFDTYLIM RYGRKDIED 
NVKEVQTHS AFKRISGNE LGCYFCNDV TAPGNSLKD 
RTLDQQCTV TRPGVSSIA GALSVELTV SLLQHEEGI 
NAPAFYKTG PQHEMNFTD DQGGVLGIL PHSIRGFLS 
SFMHVLPAT PKYNQCIAC SSIVLEEYK AKGFAFLLE 
TFNSNNYLE NLTGLSKLF ADSNYADVL ELSDEEWEN
    CDS sequence
    • >TC010974 RA
      atgattaac gaagaaggt agacggttt ttggaagag 
ttgaaaagt ggaaaggtt ttggagaag ccctatctg 
atgaacttc ttttttatt ttgtcattc agcgatctt 
aaaaagttc catttttat tattggttt gcataccca 
gttccccat aatctggaa atttcagta gaaggaatt 
agtcctatt tcagagcat tttacagag gctgaagtg 
gcgtcttta agtactgaa tatacccga ctagatcct 
caccaaaag ccatacttt ttaatccat aataaaaaa 
ctccatact taccactcg aaactgagc caagtcagt 
gagaataat tgccaggag tattatttt gcttttgca 
gattacatc aacagtggc caacatccg aaatctcaa 
ataaagaac tacattatt ttcttgtta catcattgc 
cctttcctt ggaggtaaa actttaaat tttctgttg 
ttcttcttg gaccgtaaa ttatcctgc tgtcaaagt 
ttaatctat aaattaact ctacctcaa tgcaactca 
acaatcgat gatattatt tatgggggt ggcgacaac 
gcgtgggct ggatgggaa aaaaatgaa aaaaataaa 
tttgcacca cgattttct aacatgcga acaacgatg 
gatcctcaa atattattt gaagaaagt gttgatcta 
aatttgaaa ctgatgaag tggcgtctt ctccctgat 
attaattta gataaaata aagaacgca aaatgcctt 
cttctaggc gctggaact ttgggatgt tcagtagca 
agaaatctt cttgggtgg ggtgttaga aacatcaac 
tttgtggac aattcaacg gtttcttac tcaaatcca 
gttagacaa cacctgttc acttatgaa gacgcagtt 
aaaagtaaa ccaaaggct gaagctgcg gctgaaagt 
ttgcacaag atatttccc tcaataaat tctcaaggt 
catcaattt acgatccca atgcctggg cacaatgtt 
ggtgaaagt actgttgaa tctgtgaag aaaagtgta 
gaggattta gaaaaactc attcaagaa cacgatatt 
gttttcctt ttgactgac tccagagag agccgctgg 
ttgcctaca ttactcggc atattccat aataaaata 
gtaataaat gttgctttg ggcttcgat acttacttg 
atcatgaga tatggccgc aaagatatt gaagataat 
gttaaagaa gtacaaact cattcggca tttaaaaga 
atatccgga aacgaactc ggatgttat ttttgcaat 
gatgttact gctccagga aactctcta aaggaccga 
actcttgac cagcaatgc accgtcacc agacctgga 
gtctcctca atcgcagga gctttgtct gttgagtta 
accgtttca ctgcttcaa catgaagaa ggcattaac 
gcacctgca ttttacaaa actggtccc cagcacgaa 
atgaatttt accgacgac caagggggc gttttagga 
atattacct cactcgatt agaggattc ttatcaagt 
tttatgcac gttttacct gccacacca aagtataat 
caatgcatt gcttgctct agtattgtt ctagaagag 
tacaaggcc aaaggtttt gcttttctt ctcgaaaca 
ttcaacagc aacaattat ctggagaat ttaactgga 
ctcagcaaa ttgtttgct gacagcaat tatgctgat 
gtattggaa ttaagtgat gaagaatgg gagaattaa
    • >TC010974 RB
      atgtcctca acgaagccc cttctgcaa cttgtgaca 
gtttcatca tttgtgcaa ccgagtttt tggaacaaa 
ttatccgaa ttaaaaatt aatgttgac aaattaaac 
gatgatgaa cgccaaatc tatggtttc ttctcaaac 
agccctaca acgtggacc acacatatt gtggaagtg 
gacagcaca tcatttaac acaacgctt aattcacaa 
aacaacatt ccatttcaa ggaaaaata ttcaataaa 
aacacaatt gaacagttt aaagattgt gacaaaacc 
aaaatgatt aacgaagaa ggtagacgg tttttggaa 
gagttgaaa agtggaaag gttttggag aagccctat 
ctgatgaac ttctttttt attttgtca ttcagcgat 
cttaaaaag ttccatttt tattattgg tttgcatac 
ccagttccc cataatctg gaaatttca gtagaagga 
attagtcct atttcagag cattttaca gaggctgaa 
gtggcgtct ttaagtact gaatatacc cgactagat 
cctcaccaa aagccatac tttttaatc cataataaa 
aaactccat acttaccac tcgaaactg agccaagtc 
agtgagaat aattgccag gagtattat tttgctttt 
gcagattac atcaacagt ggccaacat ccgaaatct 
caaataaag aactacatt attttcttg ttacatcat 
tgccctttc cttggaggt aaaacttta aattttctg 
ttgttcttc ttggaccgt aaattatcc tgctgtcaa 
agtttaatc tataaatta actctacct caatgcaac 
tcaacaatc gatgatatt atttatggg ggtggcgac 
aacgcgtgg gctggatgg gaaaaaaat gaaaaaaat 
aaatttgca ccacgattt tctaacatg cgaacaacg 
atggatcct caaatatta tttgaagaa agtgttgat 
ctaaatttg aaactgatg aagtggcgt cttctccct 
gatattaat ttagataaa ataaagaac gcaaaatgc 
cttcttcta ggcgctgga actttggga tgttcagta 
gcaagaaat cttcttggg tggggtgtt agaaacatc 
aactttgtg gacaattca acggtttct tactcaaat 
ccagttaga caacacctg ttcacttat gaagacgca 
gttaaaagt aaaccaaag gctgaagct gcggctgaa 
agtttgcac aagatattt ccctcaata aattctcaa 
ggtcatcaa tttacgatc ccaatgcct gggcacaat 
gttggtgaa agtactgtt gaatctgtg aagaaaagt 
gtagaggat ttagaaaaa ctcattcaa gaacacgat 
attgttttc cttttgact gactccaga gagagccgc 
tggttgcct acattactc ggcatattc cataataaa 
atagtaata aatgttgct ttgggcttc gatacttac 
ttgatcatg agatatggc cgcaaagat attgaagat 
aatgttaaa gaagtacaa actcattcg gcatttaaa 
agaatatcc ggaaacgaa ctcggatgt tatttttgc 
aatgatgtt actgctcca ggaaactct ctaaaggac 
cgaactctt gaccagcaa tgcaccgtc accagacct 
ggagtctcc tcaatcgca ggagctttg tctgttgag 
ttaaccgtt tcactgctt caacatgaa gaaggcatt 
aacgcacct gcattttac aaaactggt ccccagcac 
gaaatgaat tttaccgac gaccaaggg ggcgtttta 
ggaatatta cctcactcg attagagga ttcttatca 
agttttatg cacgtttta cctgccaca ccaaagtat 
aatcaatgc attgcttgc tctagtatt gttctagaa 
gagtacaag gccaaaggt tttgctttt cttctcgaa 
acattcaac agcaacaat tatctggag aatttaact 
ggactcagc aaattgttt gctgacagc aattatgct 
gatgtattg gaattaagt gatgaagaa tgggagaat 
taa
    mRNA sequence
    • >TC010974 RA
      acaacaaaa atgtgaggt taggttttc agttctgtc 
agtatcagc tgttcttaa aaaattcaa aaaatgtcc 
tcaacgaag ccccttctg caacttgtg acagtttca 
tcatttgtg caaccgagt ttttggaac aaattatcc 
gaattaaaa attaatgtt gacaaatta aacgatgat 
gaacgccaa atctatggt ttcttctca aacagccct 
acaacgtgg accacacat attgtggaa gtggacagc 
acatcattt aacacgtaa ttaataatg aaatttaca 
tgtatttcg ctcataatg tgttataaa ttctagaac 
gcttaattc acaaaacaa cattccatt tcaaggaaa 
aatattcaa taaaaacac aattgaaca gtttaaaga 
ttgtgacaa aaccaaaat gattaacga agaaggtag 
acggttttt ggaagagtt gaaaagtgg aaaggtttt 
ggagaagcc ctatctgat gaacttctt ttttatttt 
gtcattcag cgatcttaa aaagttcca tttttatta 
ttggtttgc atacccagt tccccataa tctggaaat 
ttcagtaga aggaattag tcctatttc agagcattt 
tacagaggc tgaagtggc gtctttaag tactgaata 
tacccgact agatcctca ccaaaagcc atacttttt 
aatccataa taaaaaact ccatactta ccactcgaa 
actgagcca agtcagtga gaataattg ccaggagta 
ttattttgc ttttgcaga ttacatcaa cagtggcca 
acatccgaa atctcaaat aaagaacta cattatttt 
cttgttaca tcattgccc tttccttgg aggtaaaac 
tttaaattt tctgttgtt cttcttgga ccgtaaatt 
atcctgctg tcaaagttt aatctataa attaactct 
acctcaatg caactcaac aatcgatga tattattta 
tgggggtgg cgacaacgc gtgggctgg atgggaaaa 
aaatgaaaa aaataaatt tgcaccacg attttctaa 
catgcgaac aacgatgga tcctcaaat attatttga 
agaaagtgt tgatctaaa tttgaaact gatgaagtg 
gcgtcttct ccctgatat taatttaga taaaataaa 
gaacgcaaa atgccttct tctaggcgc tggaacttt 
gggatgttc agtagcaag aaatcttct tgggtgggg 
tgttagaaa catcaactt tgtggacaa ttcaacggt 
ttcttactc aaatccagt tagacaaca cctgttcac 
ttatgaaga cgcagttaa aagtaaacc aaaggctga 
agctgcggc tgaaagttt gcacaagat atttccctc 
aataaattc tcaaggtca tcaatttac gatcccaat 
gcctgggca caatgttgg tgaaagtac tgttgaatc 
tgtgaagaa aagtgtaga ggatttaga aaaactcat 
tcaagaaca cgatattgt tttcctttt gactgactc 
cagagagag ccgctggtt gcctacatt actcggcat 
attccataa taaaatagt aataaatgt tgctttggg 
cttcgatac ttacttgat catgagata tggccgcaa 
agatattga agataatgt taaagaagt acaaactca 
ttcggcatt taaaagaat atccggaaa cgaactcgg 
atgttattt ttgcaatga tgttactgc tccaggaaa 
ctctctaaa ggaccgaac tcttgacca gcaatgcac 
cgtcaccag acctggagt ctcctcaat cgcaggagc 
tttgtctgt tgagttaac cgtttcact gcttcaaca 
tgaagaagg cattaacgc acctgcatt ttacaaaac 
tggtcccca gcacgaaat gaattttac cgacgacca 
agggggcgt tttaggaat attacctca ctcgattag 
aggattctt atcaagttt tatgcacgt tttacctgc 
cacaccaaa gtataatca atgcattgc ttgctctag 
tattgttct agaagagta caaggccaa aggttttgc 
ttttcttct cgaaacatt caacagcaa caattatct 
ggagaattt aactggact cagcaaatt gtttgctga 
cagcaatta tgctgatgt attggaatt aagtgatga 
agaatggga gaattaact tacaaagaa gtttcggag 
aaacgcctt aggccgcct ttgcttacg ttatttaag 
tactagcaa gcatttttt atgtacaaa cattatatg 
aatgtattt attacaatt taagaaata aatctcaat 
cgt
    • >TC010974 RB
      tctctctta ttctatgca caacaacaa aaatgtgag 
gttaggttt tcagttctg tcagtatca gctgttctt 
aaaaaattc aaaaaatgt cctcaacga agccccttc 
tgcaacttg tgacagttt catcatttg tgcaaccga 
gtttttgga acaaattat ccgaattaa aaattaatg 
ttgacaaat taaacgatg atgaacgcc aaatctatg 
gtttcttct caaacagcc ctacaacgt ggaccacac 
atattgtgg aagtggaca gcacatcat ttaacacaa 
cgcttaatt cacaaaaca acattccat ttcaaggaa 
aaatattca ataaaaaca caattgaac agtttaaag 
attgtgaca aaaccaaaa tgattaacg aagaaggta 
gacggtttt tggaagagt tgaaaagtg gaaaggttt 
tggagaagc cctatctga tgaacttct tttttattt 
tgtcattca gcgatctta aaaagttcc atttttatt 
attggtttg catacccag ttccccata atctggaaa 
tttcagtag aaggaatta gtcctattt cagagcatt 
ttacagagg ctgaagtgg cgtctttaa gtactgaat 
atacccgac tagatcctc accaaaagc catactttt 
taatccata ataaaaaac tccatactt accactcga 
aactgagcc aagtcagtg agaataatt gccaggagt 
attattttg cttttgcag attacatca acagtggcc 
aacatccga aatctcaaa taaagaact acattattt 
tcttgttac atcattgcc ctttccttg gaggtaaaa 
ctttaaatt ttctgttgt tcttcttgg accgtaaat 
tatcctgct gtcaaagtt taatctata aattaactc 
tacctcaat gcaactcaa caatcgatg atattattt 
atgggggtg gcgacaacg cgtgggctg gatgggaaa 
aaaatgaaa aaaataaat ttgcaccac gattttcta 
acatgcgaa caacgatgg atcctcaaa tattatttg 
aagaaagtg ttgatctaa atttgaaac tgatgaagt 
ggcgtcttc tccctgata ttaatttag ataaaataa 
agaacgcaa aatgccttc ttctaggcg ctggaactt 
tgggatgtt cagtagcaa gaaatcttc ttgggtggg 
gtgttagaa acatcaact ttgtggaca attcaacgg 
tttcttact caaatccag ttagacaac acctgttca 
cttatgaag acgcagtta aaagtaaac caaaggctg 
aagctgcgg ctgaaagtt tgcacaaga tatttccct 
caataaatt ctcaaggtc atcaattta cgatcccaa 
tgcctgggc acaatgttg gtgaaagta ctgttgaat 
ctgtgaaga aaagtgtag aggatttag aaaaactca 
ttcaagaac acgatattg ttttccttt tgactgact 
ccagagaga gccgctggt tgcctacat tactcggca 
tattccata ataaaatag taataaatg ttgctttgg 
gcttcgata cttacttga tcatgagat atggccgca 
aagatattg aagataatg ttaaagaag tacaaactc 
attcggcat ttaaaagaa tatccggaa acgaactcg 
gatgttatt tttgcaatg atgttactg ctccaggaa 
actctctaa aggaccgaa ctcttgacc agcaatgca 
ccgtcacca gacctggag tctcctcaa tcgcaggag 
ctttgtctg ttgagttaa ccgtttcac tgcttcaac 
atgaagaag gcattaacg cacctgcat tttacaaaa 
ctggtcccc agcacgaaa tgaatttta ccgacgacc 
aagggggcg ttttaggaa tattacctc actcgatta 
gaggattct tatcaagtt ttatgcacg ttttacctg 
ccacaccaa agtataatc aatgcattg cttgctcta 
gtattgttc tagaagagt acaaggcca aaggttttg 
cttttcttc tcgaaacat tcaacagca acaattatc 
tggagaatt taactggac tcagcaaat tgtttgctg 
acagcaatt atgctgatg tattggaat taagtgatg 
aagaatggg agaattaac ttacaaaga agtttcgga 
gaaacgcct taggccgcc tttgcttac gttatttaa 
gtactagca agcattttt tatgtacaa acattatat 
gaatgtatt tattacaat ttaagaaat aaatctcaa 
tcgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY