| General information | |||
| TC034277
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 3098421..3180526 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC034277 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04954 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04955 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: -1.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC034277 RA
MYVISPADE VVYLSYGRV IYIPSSLPT NRVTKPIRT SLSTGSHTS YPSVPSTST SSTKSRQST ASEPVFSLN KLKPTSSSK SLNQPSTSR EKSPNEPPP VRPSRKKKL KVALESARK QSFSDFLSI SREKSPKTP VNQTPEKSP TIRLFRGKS VSPKNLGRN RTAVDFDSL FIREASPGK LSVKSLNNV DETGPRLTG KQRLTPEKS SPTKKRTFF GSVRVKRIP EVIEKMAGY ESALKELTH AGYNEHLKS HNSVAFKLV RTVSDFTQQ LGQMYEQHA EELQMLVAN FRKRNGELR KERPACPSS LFHTWETLL QEVEIDSQA LGEIASILG RQVSRPLLE RSFHRKIQS RKVFTQRES YETIIAKTE EKLVKCRQE YKNAYLAYL SNPTTESLS SYFNSHNTY VQQLHATNG MLEEYGKDT LPSLLQELE EIYSDLCAA VSEAVAQGA EVVSSRAKE QCKRYDGLV SQCKSISSS SDLAHLARS LPTPTGRAP ILKRPFVPP QPPPPPDPV DGEPQDMQN LQDNIPPPL KDELVIDRL SALQVKPSH DVLKKEAAD LEAQIRQIQ DALETLLRI QQRSVEAAL YNKANELQE DISMKRFDL RVAQIHLSA INAQKELFT SKLEGDTGR ERKMSSAST ASMKNKWLK AFKSLKTPP PENTEKKNG SAATRELLK GDPDAHNFQ EYTYKKITP CDVCSQVLR GHTRQGLKC RICKMNVHL DCQDKASKC QTKQRLLRR QKSTSEIET RIQDVTAED EKSPEVDQI YQVLKQATE ISNSKPRVN VEPAPPGIT VDRTVTSNP GSSGSSGQS LNRRPGPPI QPPRAAGSN LAVVNPAPC STSSGLSRL EDLKDAFTT ILG
- >TC034277 RB
MYVISPADE VVYLSYGRV IYIPSSLPT NRVTKPIRT SLSTGSHTS YPSVPSTST SSTKSRQST ASEPVFSLN KLKPTSSSK SLNQPSTSR EKSPNEPPP VRPSRKKKL KVALESARK QSFSDFLSI SREKSPKTP VNQTPEKSP TIRLFRGKS VSPKNLGRN RTAVDFDSL FIREASPGK LSVKSLNNV DETGPRLTG KQRLTPEKS SPTKKRTFF GSVRVKRIP EVIEKMAGY ESALKELTH AGYNEHLKS HNSVAFKLV RTVSDFTQQ LGQMYEQHA EELQMLVAN FRKRNGELR KERPACPSS LFHTWETLL QEVEIDSQA LGEIASILG RQVSRPLLE RSFHRKIQS RKVFTQRES YETIIAKTE EKLVKCRQE YKNAYLAYL SNPTTESLS SYFNSHNTY VQQLHATNG MLEEYGKDT LPSLLQELE EIYSDLCAA VSEAVAQGA EVVSSRAKE QCKRYDGLV SQCKSISSS SDLAHLARS LPTPTGRAP ILKRPFVPP QPPPPPDPV DGEPQDMQN LQDNIPPPL KDELVIDRL SALQVKPSH DVLKKEAAD LEAQIRQIQ DALETLLRI QQRSVEAAL YNKANELQE DISMKRFDL RVAQIHLSA INAQKELFT SKLEGDTGR ERKMSSAST ASMKNKWLK AFKSLKTPP PENTEKKNG SAATRELLK GDPDAHNFQ EYTYKKITP CDVCSQVLR GHTRQGLKC RICKMNVHL DCQDKASKC QTKQRLLRR QKSTSEIET RIQDVTAED EKKNEMYSG MPTINRHLS VRDNNRLYP PPNAVIESP EVDQIYQVL KQATEISNS KPRVNVEPA PPGITVDRT VTSNPGSSG SSGQSLNRR PGPPIQPPR AAGSNLAVV NPAPCSTSS GLSRLEDLK DAFTTILG
- >TC034277 RA
atgtatgtg atatctccg gcagatgaa gtagtctat ctgtcatat ggtcgagtc atatacatc ccttcgtcc ctgcccaca aaccgtgtg accaaaccg attagaacc tccctgtcg accggaagt cacacgtct tatcccagt gtcccctca acgagtacc agttcaaca aagagccga caatcaact gccagcgaa ccagtcttt agccttaac aaactaaaa ccaaccagt tcgtcgaaa tccctaaac caaccctct accagccgt gaaaagtcc ccaaacgaa ccacccccg gtcagaccg tcgagaaag aaaaaactc aaagtcgcc cttgaatca gcacggaaa cagtcattt tcggatttc ctctcaatc agtcgcgaa aaatcaccc aaaacccca gtcaaccaa acgccggaa aaatcgccg accattcga ctgttccga ggaaaatca gtcagtccc aaaaaccta ggaagaaac agaacagca gtagatttc gattccctt tttatccgc gaagccagt ccgggaaag ctctccgtc aaaagtttg aataacgtt gacgagacc ggtcctagg ctgaccgga aaacagcga ctgacccca gaaaagtcg tcgcccacc aagaaaaga acgttcttt ggaagcgtt cgcgttaag agaatccct gaagtcatc gagaagatg gcgggttac gaatcggcc ttgaaagaa ctgacacat gcaggatac aacgaacac ctcaaatcc cacaactcg gtcgcgttt aaattggtg cgaaccgta tcggatttc acacaacag ctgggtcag atgtacgaa cagcatgcc gaagaatta caaatgttg gtcgcaaac tttcgaaaa cgaaacggc gaacttagg aaagaaaga cctgcgtgc ccgagttcg ttgtttcac acctgggag acgctgctt caagaggtg gaaattgat tctcaggcc ctcggcgag atcgcgtcg atcttaggg aggcaagta tcacggccg cttctggag aggtccttc cacaggaag atacagtct aggaaagtg ttcacacaa agggagagc tatgagacg atcattgcc aaaactgaa gagaagttg gttaagtgt cggcaagaa tataagaat gcctactta gcgtacctt tcgaatccc acaacggag agcctgagc tcctatttc aacagccac aacacttac gtccaacaa ctccacgcg acaaacggg atgttggaa gagtacgga aaggatact ttaccatca cttttacag gaattagag gagatctac agtgatttg tgcgcggcg gtttctgag gctgtcgct caaggggca gaagtggtc tcttcgagg gcgaaggag caatgcaag aggtatgac ggtttagtt tcccaatgc aaatccatc tcgtcgtca tcggatttg gcccactta gcgcgatct ctgcccacc cccacgggc agggccccc attcttaag agaccattc gttccacct caaccgccc ccacctccg gatcccgta gacggcgaa ccgcaagac atgcaaaac ctccaggat aacatcccc ccgcctctc aaggacgaa ctagtaata gacagacta tcggcgctc caagtgaag ccttcccac gatgttttg aaaaaggaa gcggctgat ttagaggcg caaatcaga caaatccaa gacgctcta gaaacgttg ctgaggatt cagcaacga tcggttgaa gcggcactt tataacaaa gccaacgaa ctccaagag gatatttcg atgaagcga ttcgatcta agggtggct cagatccat cttagcgcg atcaacgct cagaaagag ctttttacg agcaaactg gagggagat acaggtcgt gaaagaaaa atgtcttca gcgtcgacg gcaagcatg aaaaataaa tggttgaaa gcttttaag agtttaaaa actccaccg ccagaaaac acagagaag aaaaatggt tcggctgcc acgagagag ctcctaaaa ggtgatcca gacgctcac aatttccaa gagtataca tacaagaaa attacaccc tgcgacgta tgctcgcaa gtattgaga gggcacaca agacagggt ctgaaatgt agaatatgc aaaatgaac gttcatctc gactgtcag gacaaagcg tccaaatgt cagacgaag caacggtta ttaagacgc cagaaatca acatcagag atcgaaact cgaattcaa gacgtcacc gccgaagac gaaaagagt ccggaagtg gatcaaata taccaagtg ttgaagcaa gctaccgaa atatcaaat agcaagcca cgcgttaat gtagagccg gctccaccc ggtataacg gtggacaga accgtcact tcgaatccg ggaagttcg ggcagttca ggacaaagt cttaatcgg agaccgggt cctccgata cagcctcct agggctgcc ggctccaac ttagccgtt gtcaatcct gctccttgc tccactagc tcaggtttg tcacgacta gaggactta aaggacgcg tttacgacg attttaggc taa
- >TC034277 RB
atgtatgtg atatctccg gcagatgaa gtagtctat ctgtcatat ggtcgagtc atatacatc ccttcgtcc ctgcccaca aaccgtgtg accaaaccg attagaacc tccctgtcg accggaagt cacacgtct tatcccagt gtcccctca acgagtacc agttcaaca aagagccga caatcaact gccagcgaa ccagtcttt agccttaac aaactaaaa ccaaccagt tcgtcgaaa tccctaaac caaccctct accagccgt gaaaagtcc ccaaacgaa ccacccccg gtcagaccg tcgagaaag aaaaaactc aaagtcgcc cttgaatca gcacggaaa cagtcattt tcggatttc ctctcaatc agtcgcgaa aaatcaccc aaaacccca gtcaaccaa acgccggaa aaatcgccg accattcga ctgttccga ggaaaatca gtcagtccc aaaaaccta ggaagaaac agaacagca gtagatttc gattccctt tttatccgc gaagccagt ccgggaaag ctctccgtc aaaagtttg aataacgtt gacgagacc ggtcctagg ctgaccgga aaacagcga ctgacccca gaaaagtcg tcgcccacc aagaaaaga acgttcttt ggaagcgtt cgcgttaag agaatccct gaagtcatc gagaagatg gcgggttac gaatcggcc ttgaaagaa ctgacacat gcaggatac aacgaacac ctcaaatcc cacaactcg gtcgcgttt aaattggtg cgaaccgta tcggatttc acacaacag ctgggtcag atgtacgaa cagcatgcc gaagaatta caaatgttg gtcgcaaac tttcgaaaa cgaaacggc gaacttagg aaagaaaga cctgcgtgc ccgagttcg ttgtttcac acctgggag acgctgctt caagaggtg gaaattgat tctcaggcc ctcggcgag atcgcgtcg atcttaggg aggcaagta tcacggccg cttctggag aggtccttc cacaggaag atacagtct aggaaagtg ttcacacaa agggagagc tatgagacg atcattgcc aaaactgaa gagaagttg gttaagtgt cggcaagaa tataagaat gcctactta gcgtacctt tcgaatccc acaacggag agcctgagc tcctatttc aacagccac aacacttac gtccaacaa ctccacgcg acaaacggg atgttggaa gagtacgga aaggatact ttaccatca cttttacag gaattagag gagatctac agtgatttg tgcgcggcg gtttctgag gctgtcgct caaggggca gaagtggtc tcttcgagg gcgaaggag caatgcaag aggtatgac ggtttagtt tcccaatgc aaatccatc tcgtcgtca tcggatttg gcccactta gcgcgatct ctgcccacc cccacgggc agggccccc attcttaag agaccattc gttccacct caaccgccc ccacctccg gatcccgta gacggcgaa ccgcaagac atgcaaaac ctccaggat aacatcccc ccgcctctc aaggacgaa ctagtaata gacagacta tcggcgctc caagtgaag ccttcccac gatgttttg aaaaaggaa gcggctgat ttagaggcg caaatcaga caaatccaa gacgctcta gaaacgttg ctgaggatt cagcaacga tcggttgaa gcggcactt tataacaaa gccaacgaa ctccaagag gatatttcg atgaagcga ttcgatcta agggtggct cagatccat cttagcgcg atcaacgct cagaaagag ctttttacg agcaaactg gagggagat acaggtcgt gaaagaaaa atgtcttca gcgtcgacg gcaagcatg aaaaataaa tggttgaaa gcttttaag agtttaaaa actccaccg ccagaaaac acagagaag aaaaatggt tcggctgcc acgagagag ctcctaaaa ggtgatcca gacgctcac aatttccaa gagtataca tacaagaaa attacaccc tgcgacgta tgctcgcaa gtattgaga gggcacaca agacagggt ctgaaatgt agaatatgc aaaatgaac gttcatctc gactgtcag gacaaagcg tccaaatgt cagacgaag caacggtta ttaagacgc cagaaatca acatcagag atcgaaact cgaattcaa gacgtcacc gccgaagac gaaaaaaaa aatgaaatg tattcaggg atgcccaca attaacaga catttgtcg gtcagggat aacaacaga ttgtacccg cctcccaat gctgtcata gagagtccg gaagtggat caaatatac caagtgttg aagcaagct accgaaata tcaaatagc aagccacgc gttaatgta gagccggct ccacccggt ataacggtg gacagaacc gtcacttcg aatccggga agttcgggc agttcagga caaagtctt aatcggaga ccgggtcct ccgatacag cctcctagg gctgccggc tccaactta gccgttgtc aatcctgct ccttgctcc actagctca ggtttgtca cgactagag gacttaaag gacgcgttt acgacgatt ttaggctaa
- >TC034277 RA
agtagtcag tatagctcc ggcagcgga cggaaataa ccgcgtcct gggcgtcgc gacgcccgc gctcgtgtc gaacacagt gtgtgatag tatgagtgc gcgatgcct taggttgag gcagccaag caaagagtg agttctgaa aaatagagt aaaaattca aactgtggg acagtgttt tggtgcgtc cgtaagcaa ttctgacac ccctgttgt cgcttgaga cacattaat gttggaaac ggtgctggg ggtgctgct gtgggcagt ttcgcaatg tgggatcca atgggagca tgtatgtga tatctccgg cagatgaag tagtctatc tgtcatatg gtcgagtca tatacatcc cttcgtccc tgcccacaa accgtgtga ccaaaccga ttagaacct ccctgtcga ccggaagtc acacgtctt atcccagtg tcccctcaa cgagtacca gttcaacaa agagccgac aatcaactg ccagcgaac cagtcttta gccttaaca aactaaaac caaccagtt cgtcgaaat ccctaaacc aaccctcta ccagccgtg aaaagtccc caaacgaac cacccccgg tcagaccgt cgagaaaga aaaaactca aagtcgccc ttgaatcag cacggaaac agtcatttt cggatttcc tctcaatca gtcgcgaaa aatcaccca aaaccccag tcaaccaaa cgccggaaa aatcgccga ccattcgac tgttccgag gaaaatcag tcagtccca aaaacctag gaagaaaca gaacagcag tagatttcg attcccttt ttatccgcg aagccagtc cgggaaagc tctccgtca aaagtttga ataacgttg acgagaccg gtcctaggc tgaccggaa aacagcgac tgaccccag aaaagtcgt cgcccacca agaaaagaa cgttctttg gaagcgttc gcgttaaga gaatccctg aagtcatcg agaagatgg cgggttacg aatcggcct tgaaagaac tgacacatg caggataca acgaacacc tcaaatccc acaactcgg tcgcgttta aattggtgc gaaccgtat cggatttca cacaacagc tgggtcaga tgtacgaac agcatgccg aagaattac aaatgttgg tcgcaaact ttcgaaaac gaaacggcg aacttagga aagaaagac ctgcgtgcc cgagttcgt tgtttcaca cctgggaga cgctgcttc aagaggtgg aaattgatt ctcaggccc tcggcgaga tcgcgtcga tcttaggga ggcaagtat cacggccgc ttctggaga ggtccttcc acaggaaga tacagtcta ggaaagtgt tcacacaaa gggagagct atgagacga tcattgcca aaactgaag agaagttgg ttaagtgtc ggcaagaat ataagaatg cctacttag cgtaccttt cgaatccca caacggaga gcctgagct cctatttca acagccaca acacttacg tccaacaac tccacgcga caaacggga tgttggaag agtacggaa aggatactt taccatcac ttttacagg aattagagg agatctaca gtgatttgt gcgcggcgg tttctgagg ctgtcgctc aaggggcag aagtggtct cttcgaggg cgaaggagc aatgcaaga ggtatgacg gtttagttt cccaatgca aatccatct cgtcgtcat cggatttgg cccacttag cgcgatctc tgcccaccc ccacgggca gggccccca ttcttaaga gaccattcg ttccacctc aaccgcccc cacctccgg atcccgtag acggcgaac cgcaagaca tgcaaaacc tccaggata acatccccc cgcctctca aggacgaac tagtaatag acagactat cggcgctcc aagtgaagc cttcccacg atgttttga aaaaggaag cggctgatt tagaggcgc aaatcagac aaatccaag acgctctag aaacgttgc tgaggattc agcaacgat cggttgaag cggcacttt ataacaaag ccaacgaac tccaagagg atatttcga tgaagcgat tcgatctaa gggtggctc agatccatc ttagcgcga tcaacgctc agaaagagc tttttacga gcaaactgg agggagata caggtcgtg aaagaaaaa tgtcttcag cgtcgacgg caagcatga aaaataaat ggttgaaag cttttaaga gtttaaaaa ctccaccgc cagaaaaca cagagaaga aaaatggtt cggctgcca cgagagagc tcctaaaag gtgatccag acgctcaca atttccaag agtatacat acaagaaaa ttacaccct gcgacgtat gctcgcaag tattgagag ggcacacaa gacagggtc tgaaatgta gaatatgca aaatgaacg ttcatctcg actgtcagg acaaagcgt ccaaatgtc agacgaagc aacggttat taagacgcc agaaatcaa catcagaga tcgaaactc gaattcaag acgtcaccg ccgaagacg aaaagagtc cggaagtgg atcaaatat accaagtgt tgaagcaag ctaccgaaa tatcaaata gcaagccac gcgttaatg tagagccgg ctccacccg gtataacgg tggacagaa ccgtcactt cgaatccgg gaagttcgg gcagttcag gacaaagtc ttaatcgga gaccgggtc ctccgatac agcctccta gggctgccg gctccaact tagccgttg tcaatcctg ctccttgct ccactagct caggtttgt cacgactag aggacttaa aggacgcgt ttacgacga ttttaggct aattatgca tgcactcta tccagttat tttccggtc ccgatttcc cgatctggg gggtttaca cacttttta gcacgatct tgcacgaag agcgaaatt ttcttgata taagcacgg gcgtgttta ctgattaat tacgtgaaa tatgatagg gaattttta tcatcatta cgaggataa caatgctgt ctttcccat aaaacttgt ggtgtgatt taccaaaaa tactttaac gaactgttc tgtattcta tagttttta acaataaat aaatagatt taacagat
- >TC034277 RB
agtagtcag tatagctcc ggcagcgga cggaaataa ccgcgtcct gggcgtcgc gacgcccgc gctcgtgtc gaacacagt gtgtgatag tatgagtgc gcgatgcct taggttgag gcagccaag caaagagtg agttctgaa aaatagagt aaaaattca aactgtggg acagtgttt tggtgcgtc cgtaagcaa ttctgacac ccctgttgt cgcttgaga cacattaat gttggaaac ggtgctggg ggtgctgct gtgggcagt ttcgcaatg tgggatcca atgggagca tgtatgtga tatctccgg cagatgaag tagtctatc tgtcatatg gtcgagtca tatacatcc cttcgtccc tgcccacaa accgtgtga ccaaaccga ttagaacct ccctgtcga ccggaagtc acacgtctt atcccagtg tcccctcaa cgagtacca gttcaacaa agagccgac aatcaactg ccagcgaac cagtcttta gccttaaca aactaaaac caaccagtt cgtcgaaat ccctaaacc aaccctcta ccagccgtg aaaagtccc caaacgaac cacccccgg tcagaccgt cgagaaaga aaaaactca aagtcgccc ttgaatcag cacggaaac agtcatttt cggatttcc tctcaatca gtcgcgaaa aatcaccca aaaccccag tcaaccaaa cgccggaaa aatcgccga ccattcgac tgttccgag gaaaatcag tcagtccca aaaacctag gaagaaaca gaacagcag tagatttcg attcccttt ttatccgcg aagccagtc cgggaaagc tctccgtca aaagtttga ataacgttg acgagaccg gtcctaggc tgaccggaa aacagcgac tgaccccag aaaagtcgt cgcccacca agaaaagaa cgttctttg gaagcgttc gcgttaaga gaatccctg aagtcatcg agaagatgg cgggttacg aatcggcct tgaaagaac tgacacatg caggataca acgaacacc tcaaatccc acaactcgg tcgcgttta aattggtgc gaaccgtat cggatttca cacaacagc tgggtcaga tgtacgaac agcatgccg aagaattac aaatgttgg tcgcaaact ttcgaaaac gaaacggcg aacttagga aagaaagac ctgcgtgcc cgagttcgt tgtttcaca cctgggaga cgctgcttc aagaggtgg aaattgatt ctcaggccc tcggcgaga tcgcgtcga tcttaggga ggcaagtat cacggccgc ttctggaga ggtccttcc acaggaaga tacagtcta ggaaagtgt tcacacaaa gggagagct atgagacga tcattgcca aaactgaag agaagttgg ttaagtgtc ggcaagaat ataagaatg cctacttag cgtaccttt cgaatccca caacggaga gcctgagct cctatttca acagccaca acacttacg tccaacaac tccacgcga caaacggga tgttggaag agtacggaa aggatactt taccatcac ttttacagg aattagagg agatctaca gtgatttgt gcgcggcgg tttctgagg ctgtcgctc aaggggcag aagtggtct cttcgaggg cgaaggagc aatgcaaga ggtatgacg gtttagttt cccaatgca aatccatct cgtcgtcat cggatttgg cccacttag cgcgatctc tgcccaccc ccacgggca gggccccca ttcttaaga gaccattcg ttccacctc aaccgcccc cacctccgg atcccgtag acggcgaac cgcaagaca tgcaaaacc tccaggata acatccccc cgcctctca aggacgaac tagtaatag acagactat cggcgctcc aagtgaagc cttcccacg atgttttga aaaaggaag cggctgatt tagaggcgc aaatcagac aaatccaag acgctctag aaacgttgc tgaggattc agcaacgat cggttgaag cggcacttt ataacaaag ccaacgaac tccaagagg atatttcga tgaagcgat tcgatctaa gggtggctc agatccatc ttagcgcga tcaacgctc agaaagagc tttttacga gcaaactgg agggagata caggtcgtg aaagaaaaa tgtcttcag cgtcgacgg caagcatga aaaataaat ggttgaaag cttttaaga gtttaaaaa ctccaccgc cagaaaaca cagagaaga aaaatggtt cggctgcca cgagagagc tcctaaaag gtgatccag acgctcaca atttccaag agtatacat acaagaaaa ttacaccct gcgacgtat gctcgcaag tattgagag ggcacacaa gacagggtc tgaaatgta gaatatgca aaatgaacg ttcatctcg actgtcagg acaaagcgt ccaaatgtc agacgaagc aacggttat taagacgcc agaaatcaa catcagaga tcgaaactc gaattcaag acgtcaccg ccgaagacg aaaaaaaaa atgaaatgt attcaggga tgcccacaa ttaacagac atttgtcgg tcagggata acaacagat tgtacccgc ctcccaatg ctgtcatag agagtccgg aagtggatc aaatatacc aagtgttga agcaagcta ccgaaatat caaatagca agccacgcg ttaatgtag agccggctc cacccggta taacggtgg acagaaccg tcacttcga atccgggaa gttcgggca gttcaggac aaagtctta atcggagac cgggtcctc cgatacagc ctcctaggg ctgccggct ccaacttag ccgttgtca atcctgctc cttgctcca ctagctcag gtttgtcac gactagagg acttaaagg acgcgttta cgacgattt taggctaat tatgcatgc actctatcc agttatttt ccggtcccg atttcccga tctgggggg tttacacac tttttagca cgatcttgc acgaagagc gaaattttc ttgatataa gcacgggcg tgtttactg attaattac gtgaaatat gatagggaa tttttatca tcattacga ggataacaa tgctgtctt tcccataaa acttgtggt gtgatttac caaaaatac tttaacgaa ctgttctgt attctatag tttttaaca ataaataaa tagatttaa cagat
