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Gene information
General information
TC010967 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3206146..3314659
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010967
    iBeetle screen iB_04953

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010967 RA
      MVLNPRFDD AFCKVILFQ FALLLVHVS GYGDDKKMV 
      NDFANFRPG RLYFAIAAS PSGQTYSRA FNKTLLNIT 
      QSYLTTKVH KDTFNFTLE TLVIELPEN GSFSAALLR 
      NLCNQFEGK HVVAVLIVG DSPAAFTVS LTAKHAGIP 
      VLWARGHSG FLPGLRSLE SSPLEVQLA PTGRELVQA 
      LRGLLLQAH WHTFTLLVD PASSTALRR PELWTTLTA 
      QPLHPTLIT LSSPLRAQS LFRKLADIS RSTRGVVVL 
      LSDRPAAVR ILEDAKRLN MMDGHFVWL WVDTAANIS 
      ISDDGGADK DKPPPAAAS EDRYKRSVV KSDISDMHV 
      NYLLKNDQF LLFNRNYGV ESSKFKDRN DRSQRLFSV 
      VDGEFKGEL PAGLLRLKP LPVRVDRHL VKGTVRLLL 
      ATLKLVLQR SPLWMLQSI AKGQLTTSC WKTLGAREF 
      KFSDSFGRE LKTACKDAL AGKKFSQEI GVDKVDKSL 
      VANFEILNL VPERSTKDG DLFSNRSEN VEPSTSSVK 
      WKRVGLVSG RSVRLDTII WPGGDLSVA AVSSRARTV 
      FRVVTALAP PFVMESELD EDGQCLRGL PCHRVLTSD 
      KDNLTLVFN EMQRLEEEE DEEEELNPE YKQFANDYE 
      ENERFFPFQ KFKYRTNCC YGLSMDLLE NIAQELEFD 
      FRLYIVADG FFGSKLPNY GGKKFRYKD SDRGPPDID 
      AKWNGVVGD LVSGAAHMS FAALSVSSA RSEVIDFSV 
      PYFFSGVSL LAAPQQTSE IPLMAFLLP FSPELWIAI 
      FTSLNITAI AVAIYEWLS PFGLNPWGR QRSKNFSMS 
      SALWVMWGL LCGHLVAFK APKSWPNKF LINVWGGFS 
      VIFVASYTA NIAALIAGL FFHNTVSNY RDRILLDQR 
      VGAPRYTAA EYYVKRADY ELYDHIKKY SLENIEEGI 
      DKLRNGSLD ILIADTPIL DYYRATDHG CKLQKFGDT 
      LNEDTYAIG MTKGFPLKD SISAVIAKY SSNGYMDIL 
      QEKWYGGLP CFKLATDIA QPRPLGVAA VTGVFILLG 
      VGIALGVLI LLVEHLFFR YTLPILRNK PKGSIWRSR 
      NIMFFSQKL YRFINCVEL VSPHHAARE LVHTLRQGQ 
      ITSLFQKSI KRKEDEQRR RRKSKAQFI EMIQEIRST 
      IRRQQQEER EPPLEPVTE VDSEYQMSP DEKKSKLSP 
      SILRRTFMR SSPKAESQK IKSPTLIFN KPLFSPRSK 
      NKARSSTAL NVRRFSTDS VFNSTMKID HSTAVGRRL 
      SKDASSFLT SSPPDINSR RSSYLDIIS SGNKLSGKS 
      PVLSIENVS DSSSRSNEP LRKLSDSES IGRKLATLP 
      KYREPIERH RLQPQYSLT LGKSDETLM KSDKTELNQ 
      VSSAKSFNN VSEVSGNLS DEEIARRNK LTIEQLQAQ 
      LSKKRPPDN NAKTQNTNP QIRIQVEDT TRTEPVRTR 
      RMKTRHQSL GDSVQAPEP AKRNKLSEQ SKSLDGNPT 
      KSKKRREGD LPPAPPPPN CSPRNSNGR SPLERLSKD 
      ELVLLWRSS ESELRSHLL KAIRDKEEP VEPP
    CDS sequence
    • >TC010967 RA
      atggtcttg aatccacgc ttcgacgac gctttctgc 
      aaggttatc cttttccaa ttcgctctg ctcctggtt 
      catgtcagc ggttacgga gatgacaag aagatggtc 
      aacgacttc gccaatttt cgaccaggg cggctgtac 
      ttcgccatc gccgcctcc cccagcggc cagacgtac 
      agcagggct ttcaataag acccttctc aacattacg 
      cagtcatac ttgaccacc aaagtgcac aaagacacc 
      ttcaatttt acgctggag acgctcgtt atcgaactg 
      ccggaaaac ggaagtttc agtgcagcc cttctccgg 
      aatttgtgc aaccaattc gaagggaaa cacgtcgtt 
      gctgtactg attgtcggt gattcccct gcggccttc 
      accgtctcg ctgacggcc aaacacgcc ggcattccg 
      gtgttgtgg gcgagagga catagcgga tttctgcca 
      ggactcagg agtttggag tctagtcct ttggaggtc 
      cagctggcg ccgaccggt cgtgaatta gtgcaggcc 
      ttgagaggg ctcctgctg caagcccac tggcatact 
      ttcaccctg ctggtcgat cctgcttcg tcgacggcc 
      ttgaggcga ccggagctg tggacaaca cttactgca 
      cagccgcta catccaact ctgatcact ctgtcgtcg 
      ccattgagg gcccagtca ctctttagg aagctggcg 
      gacatttcg aggtcgacg cggggcgtg gtggtgctg 
      ctgagcgat cgtcctgca gcggtgcgt atcctggag 
      gacgccaag cggctgaac atgatggac ggccatttc 
      gtctggtta tgggtcgac acggctgcg aacatcagc 
      atcagcgac gacggtggc gccgataaa gataaacca 
      ccaccagca gcagcgagc gaggatcga tacaaacgt 
      agtgtagtt aaaagtgat atcagtgat atgcatgtg 
      aattattta ttaaaaaac gatcagttc cttttgttc 
      aatcgtaat tatggcgtt gaaagttct aaattcaaa 
      gatcgtaac gatagatct caacgttta tttagtgtt 
      gtggatggt gagtttaaa ggagaactt ccggccggt 
      ctgttgcgt ctgaaaccc cttccggtc agggtggac 
      agacacctg gtcaagggc accgtgcgg ttgctcctc 
      gccactctc aaattggtc ctccaaagg tcgcctttg 
      tggatgttg caaagcatc gccaaagga cagctcacc 
      accagctgt tggaaaacg ctaggtgcc agggaattc 
      aagttctcc gacagcttt ggaagggaa ctgaagacg 
      gcctgcaag gacgccctc gccggcaaa aaattttca 
      caagaaatt ggcgttgac aaagtggac aaatcatta 
      gttgcaaat ttcgaaata ttaaactta gttcctgaa 
      cgtagcacc aaagacggc gacttattt agcaataga 
      agcgagaac gtcgagccg agtacttcg agcgtcaag 
      tggaaaaga gttggcttg gtctccggc agatcggtt 
      cgtttggac acgatcatc tggcctgga ggtgacctc 
      tcggtggcg gcagtgtcg tcaagggcc agaactgtt 
      ttcagagtg gttacggcc ttggcgcca cccttcgtc 
      atggaaagc gagttggac gaggatgga cagtgcctg 
      agaggtctt ccgtgtcat cgagtgctg acatcggac 
      aaagacaac ttgacgttg gtttttaac gagatgcag 
      cgtcttgaa gaggaagaa gacgaggag gaagaacta 
      aacccagag tacaaacaa ttcgccaac gattacgaa 
      gaaaatgag cgatttttt ccgttccaa aaattcaaa 
      tatcgcaca aattgctgt tacggcctt tcaatggac 
      ctcctcgag aacatcgcc caagaactc gagttcgat 
      tttcgcttg tacattgtc gcggacggt ttcttcggc 
      tctaagttg cctaattac ggtggcaaa aaattccgg 
      tacaaagac agcgaccgg gggcccccc gatattgac 
      gccaagtgg aacggcgtc gtgggggac ctggtgtcg 
      ggggcggcg cacatgtcc ttcgcggcc ttgagcgtc 
      tccagtgca cgcagtgaa gttatagac ttcagtgtt 
      ccgtatttc ttcagcggg gtgtcgttg ctcgccgcc 
      cctcaacag acctctgaa ataccgttg atggccttt 
      ctgttaccg ttttcgccc gagttgtgg attgctatt 
      tttacgagt ctcaatatc acggcaatc gcggtcgcg 
      atctacgaa tggctgtcg ccgttcggg ctcaacccc 
      tgggggcgg cagcgctcc aagaacttc agcatgagt 
      tcggcgctt tgggtcatg tgggggctg ctgtgcggg 
      cacctggtg gctttcaag gctccgaaa agttggcct 
      aataagttt ctcatcaac gtctggggc ggattttcg 
      gttatcttt gtcgcaagt tacacagcc aacattgcg 
      gcgttgatt gccggcctc ttcttccac aataccgtc 
      agcaattac agggaccgg atcttgttg gatcaaaga 
      gtgggcgct ccccgatat acagcagcc gaatattac 
      gtcaaaaga gcggattat gaactttac gaccatata 
      aaaaaatat tcgctggag aacatcgaa gaaggcatc 
      gataaattg cgtaacggt tctttggat attctcatc 
      gcagacact ccaattttg gactattat cgcgccacc 
      gatcacggc tgcaagctc cagaaattc ggagatacg 
      ctaaacgaa gacacttac gcaatagga atgaccaaa 
      ggtttccct ttgaaggat agcatttca gccgtgata 
      gcaaagtat tccagcaac ggctacatg gacatcctg 
      caggaaaag tggtacgga ggcctgccg tgcttcaag 
      ctcgcgaca gatattgcc caacctaga cctctcggc 
      gtcgctgcc gtcaccggg gtattcatc ctcctggga 
      gttggcatc gcactcggc gtcctaatc ttgctggtc 
      gaacattta tttttccgc tatacttta cccatcctt 
      cgaaataag cccaagggc tccatctgg cggagcagg 
      aatatcatg tttttcagt cagaaactt tatcggttt 
      atcaattgc gtcgaattg gtgtcgcct catcatgcg 
      gcccgagaa ctagttcac accctacga caaggacag 
      attaccagt ttgttccaa aaaagcatc aaaaggaag 
      gaagacgaa cagcgtagg agaaggaag agcaaagcg 
      cagtttatc gaaatgatc caggaaatt aggagcacg 
      attcgcagg cagcagcag gaggagcga gagccgcct 
      ctcgaaccg gtaacggaa gtcgactcg gaatatcag 
      atgtctccc gacgagaaa aaatccaag ttaagtccc 
      agtatatta cggagaacg ttcatgcgc tccagtccc 
      aaggcagag agtcagaaa attaaatcc ccgacactg 
      atcttcaac aagccgttg ttcagtccg agatccaag 
      aacaaagcg agaagttca acggccttg aacgtgcgc 
      cgattcagc accgacagt gttttcaat tcgaccatg 
      aaaatcgac cacagtacc gcagtgggg cggcgtttg 
      agcaaagac gcttcgagt ttcctgacg agcagtccg 
      cccgacata aacagccgt agatccagc tatttggac 
      atcataagc tccgggaat aaattatcc ggcaaaagt 
      ccggttctc tccatagaa aacgtttca gactccagc 
      tcgcgatcc aacgaaccg ttgcgaaaa ctgtcggat 
      tcggagagc atagggcgg aagctggca actcttccg 
      aaatatcgc gaaccgatt gaacgccat cgcttgcaa 
      ccccagtac agcctaacc ttgggcaag agcgacgaa 
      actctcatg aaatcagac aaaacagaa ctgaaccaa 
      gtatcttca gcgaaaagt tttaataac gtgtcagaa 
      gtcagtggc aatttatcg gacgaggag atagcccgt 
      cggaataaa ttgaccata gagcagctc caagcgcaa 
      ttgagcaag aaacgaccg ccagacaat aacgccaaa 
      acgcaaaac accaaccca cagattagg atacaagta 
      gaggacacg acgaggacc gaaccggtg aggacccgt 
      cggatgaag acacgccac cagagcctg ggggactcg 
      gtgcaagca ccggaacca gccaaaagg aacaagttg 
      tccgagcag tcgaagtcg cttgacgga aaccccacc 
      aagtcgaag aagagacga gagggggat ctgcccccg 
      gcgccgccg ccccccaac tgctcgccg cggaactcc 
      aacggaagg tcgccgctg gagaggttg tccaaggac 
      gagctggtg ctgctgtgg aggagctcc gagtcggag 
      ttgagaagc catctgttg aaggccatc agggataaa 
      gaggaaccg gtggagccg ccttga
    mRNA sequence
    • >TC010967 RA
      aaaaatcaa gcctaacct tgggggtct tggcgcgcc 
      agcgccccg aagggcgtc gcctctcgg aggccccat 
      cgcgtcttc agactcagc agcgcactt gtgccccaa 
      cagtggcgg agtcatggt cgtgatgat gcaaaacgc 
      gaaaacaaa agaaaaatc acgtctcgt gttaaggga 
      tgggtccca tcgattcgg cccaagcgc atgcgcaca 
      gcctccgtc cgacggggg cccgtcgct tattccgtg 
      ccgcgagaa tcaagtgtg ccgtggccc gaattctgc 
      aacccgatc atcgaagtc ggccgtgac ggaccgtag 
      taaacgcgc cgcaaaatc gaacaaatc gctaaaacg 
      ggacgagtt ttcgacgct ttttccggg cgatttttc 
      cggaaggct ttggacgtc ttggggatg gtgcgctgg 
      tgaggaccc ggattattg agatgcgga acgggatat 
      gaagcggcg atttccgga ggtttttaa gacgatggg 
      gctgaagca aaggagaca ccgcctgaa aacatgatt 
      ccattcaaa tggagcaaa cccaaattc aataaagtg 
      aaattcaag taaaatcag tcaaatatc aaacattcc 
      gccttaccc aagtaacca aagcaataa aaaccagga 
      caaaattaa aaatttatt ccaatcatg tcgaaaact 
      ctgattctg atcatcgat ttggacacg ataatgcgc 
      cgtaatatc ttcaagaat tgcaatatc tcccaatcg 
      acctcaccg aaagcatga ccgtgtaaa gcgcccccc 
      tttcaactc ctcaccccc aaacctaga aagatggtc 
      ttgaatcca cgcttcgac gacgctttc tgcaaggtt 
      atccttttc caattcgct ctgctcctg gttcatgtc 
      agcggttac ggagatgac aagaagatg gtcaacgac 
      ttcgccaat tttcgacca gggcggctg tacttcgcc 
      atcgccgcc tcccccagc ggccagacg tacagcagg 
      gctttcaat aagaccctt ctcaacatt acgcagtca 
      tacttgacc accaaagtg cacaaagac accttcaat 
      tttacgctg gagacgctc gttatcgaa ctgccggaa 
      aacggaagt ttcagtgca gcccttctc cggaatttg 
      tgcaaccaa ttcgaaggg aaacacgtc gttgctgta 
      ctgattgtc ggtgattcc cctgcggcc ttcaccgtc 
      tcgctgacg gccaaacac gccggcatt ccggtgttg 
      tgggcgaga ggacatagc ggatttctg ccaggactc 
      aggagtttg gagtctagt cctttggag gtccagctg 
      gcgccgacc ggtcgtgaa ttagtgcag gccttgaga 
      gggctcctg ctgcaagcc cactggcat actttcacc 
      ctgctggtc gatcctgct tcgtcgacg gccttgagg 
      cgaccggag ctgtggaca acacttact gcacagccg 
      ctacatcca actctgatc actctgtcg tcgccattg 
      agggcccag tcactcttt aggaagctg gcggacatt 
      tcgaggtcg acgcggggc gtggtggtg ctgctgagc 
      gatcgtcct gcagcggtg cgtatcctg gaggacgcc 
      aagcggctg aacatgatg gacggccat ttcgtctgg 
      ttatgggtc gacacggct gcgaacatc agcatcagc 
      gacgacggt ggcgccgat aaagataaa ccaccacca 
      gcagcagcg agcgaggat cgatacaaa cgtagtgta 
      gttaaaagt gatatcagt gatatgcat gtgaattat 
      ttattaaaa aacgatcag ttccttttg ttcaatcgt 
      aattatggc gttgaaagt tctaaattc aaagatcgt 
      aacgataga tctcaacgt ttatttagt gttgtggat 
      ggtgagttt aaaggagaa cttccggcc ggtctgttg 
      cgtctgaaa ccccttccg gtcagggtg gacagacac 
      ctggtcaag ggcaccgtg cggttgctc ctcgccact 
      ctcaaattg gtcctccaa aggtcgcct ttgtggatg 
      ttgcaaagc atcgccaaa ggacagctc accaccagc 
      tgttggaaa acgctaggt gccagggaa ttcaagttc 
      tccgacagc tttggaagg gaactgaag acggcctgc 
      aaggacgcc ctcgccggc aaaaaattt tcacaagaa 
      attggcgtt gacaaagtg gacaaatca ttagttgca 
      aatttcgaa atattaaac ttagttcct gaacgtagc 
      accaaagac ggcgactta tttagcaat agaagcgag 
      aacgtcgag ccgagtact tcgagcgtc aagtggaaa 
      agagttggc ttggtctcc ggcagatcg gttcgtttg 
      gacacgatc atctggcct ggaggtgac ctctcggtg 
      gcggcagtg tcgtcaagg gccagaact gttttcaga 
      gtggttacg gccttggcg ccacccttc gtcatggaa 
      agcgagttg gacgaggat ggacagtgc ctgagaggt 
      cttccgtgt catcgagtg ctgacatcg gacaaagac 
      aacttgacg ttggttttt aacgagatg cagcgtctt 
      gaagaggaa gaagacgag gaggaagaa ctaaaccca 
      gagtacaaa caattcgcc aacgattac gaagaaaat 
      gagcgattt tttccgttc caaaaattc aaatatcgc 
      acaaattgc tgttacggc ctttcaatg gacctcctc 
      gagaacatc gcccaagaa ctcgagttc gattttcgc 
      ttgtacatt gtcgcggac ggtttcttc ggctctaag 
      ttgcctaat tacggtggc aaaaaattc cggtacaaa 
      gacagcgac cgggggccc cccgatatt gacgccaag 
      tggaacggc gtcgtgggg gacctggtg tcgggggcg 
      gcgcacatg tccttcgcg gccttgagc gtctccagt 
      gcacgcagt gaagttata gacttcagt gttccgtat 
      ttcttcagc ggggtgtcg ttgctcgcc gcccctcaa 
      cagacctct gaaataccg ttgatggcc tttctgtta 
      ccgttttcg cccgagttg tggattgct atttttacg 
      agtctcaat atcacggca atcgcggtc gcgatctac 
      gaatggctg tcgccgttc gggctcaac ccctggggg 
      cggcagcgc tccaagaac ttcagcatg agttcggcg 
      ctttgggtc atgtggggg ctgctgtgc gggcacctg 
      gtggctttc aaggctccg aaaagttgg cctaataag 
      tttctcatc aacgtctgg ggcggattt tcggttatc 
      tttgtcgca agttacaca gccaacatt gcggcgttg 
      attgccggc ctcttcttc cacaatacc gtcagcaat 
      tacagggac cggatcttg ttggatcaa agagtgggc 
      gctccccga tatacagca gccgaatat tacgtcaaa 
      agagcggat tatgaactt tacgaccat ataaaaaaa 
      tattcgctg gagaacatc gaagaaggc atcgataaa 
      ttgcgtaac ggttctttg gatattctc atcgcagac 
      actccaatt ttggactat tatcgcgcc accgatcac 
      ggctgcaag ctccagaaa ttcggagat acgctaaac 
      gaagacact tacgcaata ggaatgacc aaaggtttc 
      cctttgaag gatagcatt tcagccgtg atagcaaag 
      tattccagc aacggctac atggacatc ctgcaggaa 
      aagtggtac ggaggcctg ccgtgcttc aagctcgcg 
      acagatatt gcccaacct agacctctc ggcgtcgct 
      gccgtcacc ggggtattc atcctcctg ggagttggc 
      atcgcactc ggcgtccta atcttgctg gtcgaacat 
      ttatttttc cgctatact ttacccatc cttcgaaat 
      aagcccaag ggctccatc tggcggagc aggaatatc 
      atgtttttc agtcagaaa ctttatcgg tttatcaat 
      tgcgtcgaa ttggtgtcg cctcatcat gcggcccga 
      gaactagtt cacacccta cgacaagga cagattacc 
      agtttgttc caaaaaagc atcaaaagg aaggaagac 
      gaacagcgt aggagaagg aagagcaaa gcgcagttt 
      atcgaaatg atccaggaa attaggagc acgattcgc 
      aggcagcag caggaggag cgagagccg cctctcgaa 
      ccggtaacg gaagtcgac tcggaatat cagatgtct 
      cccgacgag aaaaaatcc aagttaagt cccagtata 
      ttacggaga acgttcatg cgctccagt cccaaggca 
      gagagtcag aaaattaaa tccccgaca ctgatcttc 
      aacaagccg ttgttcagt ccgagatcc aagaacaaa 
      gcgagaagt tcaacggcc ttgaacgtg cgccgattc 
      agcaccgac agtgttttc aattcgacc atgaaaatc 
      gaccacagt accgcagtg gggcggcgt ttgagcaaa 
      gacgcttcg agtttcctg acgagcagt ccgcccgac 
      ataaacagc cgtagatcc agctatttg gacatcata 
      agctccggg aataaatta tccggcaaa agtccggtt 
      ctctccata gaaaacgtt tcagactcc agctcgcga 
      tccaacgaa ccgttgcga aaactgtcg gattcggag 
      agcataggg cggaagctg gcaactctt ccgaaatat 
      cgcgaaccg attgaacgc catcgcttg caaccccag 
      tacagccta accttgggc aagagcgac gaaactctc 
      atgaaatca gacaaaaca gaactgaac caagtatct 
      tcagcgaaa agttttaat aacgtgtca gaagtcagt 
      ggcaattta tcggacgag gagatagcc cgtcggaat 
      aaattgacc atagagcag ctccaagcg caattgagc 
      aagaaacga ccgccagac aataacgcc aaaacgcaa 
      aacaccaac ccacagatt aggatacaa gtagaggac 
      acgacgagg accgaaccg gtgaggacc cgtcggatg 
      aagacacgc caccagagc ctgggggac tcggtgcaa 
      gcaccggaa ccagccaaa aggaacaag ttgtccgag 
      cagtcgaag tcgcttgac ggaaacccc accaagtcg 
      aagaagaga cgagagggg gatctgccc ccggcgccg 
      ccgcccccc aactgctcg ccgcggaac tccaacgga 
      aggtcgccg ctggagagg ttgtccaag gacgagctg 
      gtgctgctg tggaggagc tccgagtcg gagttgaga 
      agccatctg ttgaaggcc atcagggat aaagaggaa 
      ccggtggag ccgccttga acacgcggc agtggagta 
      ccgggcacc tgatggtat ttaaaactt aaatttata 
      gtaaatgat catcatcga ccattatct catctcgat 
      tatttcgta gacttttgc gcaagtcgt tgattcagg 
      tttaggcac gatcggatc ggagttgtg cctatgaaa 
      tgaagaaac ggccttaga tttgtagag tgataagaa 
      acgaccact aagtgttat tagaagcct ttatcttta 
      gaggtcgct cagacctca acgcctttt aactaggac 
      tggttctct ttgtaggga ttttaacaa ttggttatc 
      gtcactgat tcggtttca ctttgattg ctgtcatgt 
      attattgga gatatattt aacgtataa tcaaactga 
      gtttcataa atttgaata atatatact gccaattat 
      attagaatt ttttaagta gattggtga tattaaagt 
      cttatgcaa aatgaaaca aaggttata agagccaac 
      gttggcgat agcatagat acaaaaaca tattatagt 
      gttgttgtt taaacgcgg gcagcatca cattcaaac 
      catttatca taaaatcga gtcgaaacg tctagcttt 
      aaggaattc aaattttat cttaacttt ggttaacgt 
      gacgctgtc caagttttt gagtcaatt ttaaagatt 
      tctctccgt ctactcatt aggtataag tatttctct 
      atcctattg ttgatattt taaagttta ttggaattg 
      aataatata atttcaaag atatgtata ttaaaacaa 
      accatatga cttgtgatg tttatagat accatttgt 
      ttgaaatat tttcgtaaa tcttaaaca cattaacat 
      taaatcaca cttttaaaa tgtgacggt agttgagct 
      ctctaaatg tctatatga tataaatct tatctaaac 
      ttgaatgac atattccag aaaatattt taaacaaaa 
      ttttgttgt acatattgt acatagcta cttaagtta 
      tggtcacaa atttgtaaa ttatagcta atttaaaaa 
      cactttatt gttgacaaa tatatgact tagaataaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY