| General information | |||
| TC010961 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 3427580..3433828 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC010961 | ||
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04951 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (11 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 4 (16 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 1 (19 dpi)
- >TC010961 RA
MQDIVCEQC GGVDFYKEA GFYFCSGCQ IQSQEVQEY VYDEYTPET RLKTKKIQS DKTAKTENK ITSWECYNY ILLGLTNEL INLGANKKL KAVVRLLWM RYLEKLQVF KLGRDELPK LQAVCSNMD AEIVYGKLA KKKRKRNKS SSSELDVTS LDSTSLKHE RVKRKRALA KAQYEEFSK KSQETPSLH NETLTSLQT GSEKSSKNA VPIKYNIYA TKELRKIKS SQYHWRKHR RDFERNLKC HKLSYRKIS GKYRASPYI LSINKLYAI LYLGLLITK SKVQLSDIL RFIKEGHLS FNYYSHFFP EDFVDKTLN FRNFKKNTM FVNAPFRTI AAEMSVLLQ IDFYISCPN LIELCERYC GELNLPREI CDCAKNIIT RADIKMSFR KRNGKIPNY EGRVLSVIL FVLKLLFGL DDVTEFHLA NYSNILNNS EAVRPKMFN IIDWLKYIE YRDLVMNNQ HFPTSFHDE NNFNNVNLA LSALGRKDY SASAKPNST SDNYKQLFE KLKNYLDVP DFIEFPVTF TPFLDYAKQ LPKSDYLEI LSRDFSSDS LAHVLKPIK FLQLVNEPE VVNGGANED WVVEKLTCF KEEQHREKL NRRKLVTVK LSFDTKVKP TKETLCEDA TKLKTHFQE ANERHFKRN LRQLNYKNY LVVDYDLKQ IEADLESSA DSYPRHYNP YERFWMRSR IGIEDMSHE QCRDFVKKF PYNFRLVFK ECRRIAEQN DQEFLVEYN FTELYLIHM ANFGKKKQT IIKNKLIAH YLDKNVSLW
- >TC010961 RA
atgcaggac atagtgtgt gagcagtgc ggtggcgtc gatttctac aaagaagct ggtttctac ttctgcagc gggtgtcag attcagtct caggaggtc caagagtac gtttacgat gaatacacc cccgaaact cgcctaaag actaaaaaa atccaaagt gataagaca gcgaagact gagaataaa ataacaagt tgggagtgt tacaactac attcttctt ggtcttacg aatgaactt ataaatttg ggggctaat aaaaagttg aaggcggtc gttcgtctc ttgtggatg cgttatttg gaaaaattg caagttttc aaattgggg cgtgatgag ttaccgaaa ttgcaagcc gtctgtagc aacatggac gcagaaatt gtgtatgga aaactggcg aagaagaaa cgaaagcga aacaaatcg tcgtcaagc gaacttgac gtcacatct ttagattca acttctttg aagcatgaa agggtcaag agaaagcgt gctttagcc aaggctcaa tatgaggaa ttcagtaaa aagtctcaa gagacacct tcactgcat aatgaaact ttgacaagt ctgcaaaca ggttctgag aaaagttct aaaaatgca gtacctatc aaatacaat atttatgct actaaagag ttgagaaag attaaaagc tcacaatac cactggcgt aagcaccga agggacttt gaacgaaat ttaaaatgt cacaagttg agttataga aaaatctcg gggaaatac cgggctagc ccttacata ctttcaata aacaaactt tacgctatt ttataccta ggattgctt attactaaa agcaaggtg caactgagc gacattttg aggttcatt aaagagggg catttgagt ttcaattat tacagtcat ttttttccc gaagatttc gtcgataaa acactcaat tttcgtaat tttaagaaa aacacaatg tttgtgaat gcaccattt cgaacaatt gctgccgaa atgtcggtt ttattacaa attgatttt tatatttcg tgtcctaat ttaattgag ttgtgtgaa cgctattgt ggggagttg aatttgcct cgtgagatt tgcgattgc gccaaaaat attatcaca agggctgat attaaaatg tcgtttcgt aaacgaaat ggcaaaatc ccaaattac gaaggtcgc gttttaagc gttattctc tttgtgctc aaattatta tttggatta gacgatgtc acagagttt catttagca aactactcg aatatttta aataatagt gaagctgtt agacctaaa atgtttaac ataatcgac tggcttaaa tacatagaa tatagggac ttggtgatg aataatcag cactttccc acaagtttt cacgacgag aacaatttc aacaatgta aacttagcg ttaagtgca cttggaaga aaagattat tcagcatca gcaaaaccg aactcgacc tcagataac tacaagcaa ctatttgaa aaattgaaa aactatctt gacgtccct gactttatc gaatttccg gttacgttc actccgttt ttggattat gctaaacag ttgcctaaa agcgattat ttggaaatt ttatctaga gatttttcc agcgatagt ctagcgcac gtattgaaa ccaatcaaa tttttgcaa cttgttaac gaaccggaa gttgttaat ggcggagct aacgaggac tgggttgtc gaaaaactt acttgcttc aaagaagaa caacacagg gaaaaatta aacagacgg aaacttgta actgttaaa cttagtttt gatacaaaa gttaagccg acaaaagaa actttgtgt gaagacgct acgaagttg aaaactcat ttccaggaa gccaatgaa aggcacttt aagcgaaat ttgcgccaa ttgaattat aaaaattat ttggttgtt gactacgat ttaaaacaa attgaagcc gacttagaa tcgtccgca gatagttat cctaggcat tacaaccct tatgaaagg ttttggatg cggtctcgg atcggtatt gaggatatg agtcatgaa cagtgtcgt gattttgtc aaaaaattt ccatataac tttcgttta gttttcaag gaatgtcgg agaatagct gaacagaac gatcaggag tttttggtc gaatacaat tttactgaa ctttactta attcacatg gcaaatttt gggaaaaaa aagcaaaca attataaaa aataaactc atcgctcac tatctggat aaaaatgtt tcgttgtgg tga
- >TC010961 RA
agactctcg tataattat aattgcgtg gttaattgg ttaataata atttgcaaa gtttgtccg acataaccc cacacttgc acgtgtgag ccctaaaat gcaggacat agtgtgtga gcagtgcgg tggcgtcga tttctacaa agaagctgg tttctactt ctgcagcgg gtgtcagat tcagtctca ggaggtcca agagtacgt ttacgatga atacacccc cgaaactcg cctaaagac taaaaaaat ccaaagtga taagacagc gaagactga gaataaaat aacaagttg ggagtgtta caactacat tcttcttgg tcttacgaa tgaacttat aaatttggg ggctaataa aaagttgaa ggcggtcgt tcgtctctt gtggatgcg ttatttgga aaaattgca agttttcaa attggggcg tgatgagtt accgaaatt gcaagccgt ctgtagcaa catggacgc agaaattgt gtatggaaa actggcgaa gaagaaacg aaagcgaaa caaatcgtc gtcaagcga acttgacgt cacatcttt agattcaac ttctttgaa gcatgaaag ggtcaagag aaagcgtgc tttagccaa ggctcaata tgaggaatt cagtaaaaa gtctcaaga gacaccttc actgcataa tgaaacttt gacaagtct gcaaacagg ttctgagaa aagttctaa aaatgcagt acctatcaa atacaatat ttatgctac taaagagtt gagaaagat taaaagctc acaatacca ctggcgtaa gcaccgaag ggactttga acgaaattt aaaatgtca caagttgag ttatagaaa aatctcggg gaaataccg ggctagccc ttacatact ttcaataaa caaacttta cgctatttt atacctagg attgcttat tactaaaag caaggtgca actgagcga cattttgag gttcattaa agaggggca tttgagttt caattatta cagtcattt ttttcccga agatttcgt cgataaaac actcaattt tcgtaattt taagaaaaa cacaatgtt tgtgaatgc accatttcg aacaattgc tgccgaaat gtcggtttt attacaaat tgattttta tatttcgtg tcctaattt aattgagtt gtgtgaacg ctattgtgg ggagttgaa tttgcctcg tgagatttg cgattgcgc caaaaatat tatcacaag ggctgatat taaaatgtc gtttcgtaa acgaaatgg caaaatccc aaattacga aggtcgcgt tttaagcgt tattctctt tgtgctcaa attattatt tggattaga cgatgtcac agagtttca tttagcaaa ctactcgaa tattttaaa taatagtga agctgttag acctaaaat gtttaacat aatcgactg gcttaaata catagaata tagggactt ggtgatgaa taatcagca ctttcccac aagttttca cgacgagaa caatttcaa caatgtaaa cttagcgtt aagtgcact tggaagaaa agattattc agcatcagc aaaaccgaa ctcgacctc agataacta caagcaact atttgaaaa attgaaaaa ctatcttga cgtccctga ctttatcga atttccggt tacgttcac tccgttttt ggattatgc taaacagtt gcctaaaag cgattattt ggaaatttt atctagaga tttttccag cgatagtct agcgcacgt attgaaacc aatcaaatt tttgcaact tgttaacga accggaagt tgttaatgg cggagctaa cgaggactg ggttgtcga aaaacttac ttgcttcaa agaagaaca acacaggga aaaattaaa cagacggaa acttgtaac tgttaaact tagttttga tacaaaagt taagccgac aaaagaaac tttgtgtga agacgctac gaagttgaa aactcattt ccaggaagc caatgaaag gcactttaa gcgaaattt gcgccaatt gaattataa aaattattt ggttgttga ctacgattt aaaacaaat tgaagccga cttagaatc gtccgcaga tagttatcc taggcatta caaccctta tgaaaggtt ttggatgcg gtctcggat cggtattga ggatatgag tcatgaaca gtgtcgtga ttttgtcaa aaaatttcc atataactt tcgtttagt tttcaagga atgtcggag aatagctga acagaacga tcaggagtt tttggtcga atacaattt tactgaact ttacttaat tcacatggc aaattttgg gaaaaaaaa gcaaacaat tataaaaaa taaactcat cgctcacta tctggataa aaatgtttc gttgtggtg attttcaat ttttgggat gttttattt ttaataaat tacttcaca ttat
