Status
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Gene information
General information
TC010961 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3427580..3433828
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010961
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04951

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010961 RA
      MQDIVCEQC GGVDFYKEA GFYFCSGCQ IQSQEVQEY 
      VYDEYTPET RLKTKKIQS DKTAKTENK ITSWECYNY 
      ILLGLTNEL INLGANKKL KAVVRLLWM RYLEKLQVF 
      KLGRDELPK LQAVCSNMD AEIVYGKLA KKKRKRNKS 
      SSSELDVTS LDSTSLKHE RVKRKRALA KAQYEEFSK 
      KSQETPSLH NETLTSLQT GSEKSSKNA VPIKYNIYA 
      TKELRKIKS SQYHWRKHR RDFERNLKC HKLSYRKIS 
      GKYRASPYI LSINKLYAI LYLGLLITK SKVQLSDIL 
      RFIKEGHLS FNYYSHFFP EDFVDKTLN FRNFKKNTM 
      FVNAPFRTI AAEMSVLLQ IDFYISCPN LIELCERYC 
      GELNLPREI CDCAKNIIT RADIKMSFR KRNGKIPNY 
      EGRVLSVIL FVLKLLFGL DDVTEFHLA NYSNILNNS 
      EAVRPKMFN IIDWLKYIE YRDLVMNNQ HFPTSFHDE 
      NNFNNVNLA LSALGRKDY SASAKPNST SDNYKQLFE 
      KLKNYLDVP DFIEFPVTF TPFLDYAKQ LPKSDYLEI 
      LSRDFSSDS LAHVLKPIK FLQLVNEPE VVNGGANED 
      WVVEKLTCF KEEQHREKL NRRKLVTVK LSFDTKVKP 
      TKETLCEDA TKLKTHFQE ANERHFKRN LRQLNYKNY 
      LVVDYDLKQ IEADLESSA DSYPRHYNP YERFWMRSR 
      IGIEDMSHE QCRDFVKKF PYNFRLVFK ECRRIAEQN 
      DQEFLVEYN FTELYLIHM ANFGKKKQT IIKNKLIAH 
      YLDKNVSLW
    CDS sequence
    • >TC010961 RA
      atgcaggac atagtgtgt gagcagtgc ggtggcgtc 
      gatttctac aaagaagct ggtttctac ttctgcagc 
      gggtgtcag attcagtct caggaggtc caagagtac 
      gtttacgat gaatacacc cccgaaact cgcctaaag 
      actaaaaaa atccaaagt gataagaca gcgaagact 
      gagaataaa ataacaagt tgggagtgt tacaactac 
      attcttctt ggtcttacg aatgaactt ataaatttg 
      ggggctaat aaaaagttg aaggcggtc gttcgtctc 
      ttgtggatg cgttatttg gaaaaattg caagttttc 
      aaattgggg cgtgatgag ttaccgaaa ttgcaagcc 
      gtctgtagc aacatggac gcagaaatt gtgtatgga 
      aaactggcg aagaagaaa cgaaagcga aacaaatcg 
      tcgtcaagc gaacttgac gtcacatct ttagattca 
      acttctttg aagcatgaa agggtcaag agaaagcgt 
      gctttagcc aaggctcaa tatgaggaa ttcagtaaa 
      aagtctcaa gagacacct tcactgcat aatgaaact 
      ttgacaagt ctgcaaaca ggttctgag aaaagttct 
      aaaaatgca gtacctatc aaatacaat atttatgct 
      actaaagag ttgagaaag attaaaagc tcacaatac 
      cactggcgt aagcaccga agggacttt gaacgaaat 
      ttaaaatgt cacaagttg agttataga aaaatctcg 
      gggaaatac cgggctagc ccttacata ctttcaata 
      aacaaactt tacgctatt ttataccta ggattgctt 
      attactaaa agcaaggtg caactgagc gacattttg 
      aggttcatt aaagagggg catttgagt ttcaattat 
      tacagtcat ttttttccc gaagatttc gtcgataaa 
      acactcaat tttcgtaat tttaagaaa aacacaatg 
      tttgtgaat gcaccattt cgaacaatt gctgccgaa 
      atgtcggtt ttattacaa attgatttt tatatttcg 
      tgtcctaat ttaattgag ttgtgtgaa cgctattgt 
      ggggagttg aatttgcct cgtgagatt tgcgattgc 
      gccaaaaat attatcaca agggctgat attaaaatg 
      tcgtttcgt aaacgaaat ggcaaaatc ccaaattac 
      gaaggtcgc gttttaagc gttattctc tttgtgctc 
      aaattatta tttggatta gacgatgtc acagagttt 
      catttagca aactactcg aatatttta aataatagt 
      gaagctgtt agacctaaa atgtttaac ataatcgac 
      tggcttaaa tacatagaa tatagggac ttggtgatg 
      aataatcag cactttccc acaagtttt cacgacgag 
      aacaatttc aacaatgta aacttagcg ttaagtgca 
      cttggaaga aaagattat tcagcatca gcaaaaccg 
      aactcgacc tcagataac tacaagcaa ctatttgaa 
      aaattgaaa aactatctt gacgtccct gactttatc 
      gaatttccg gttacgttc actccgttt ttggattat 
      gctaaacag ttgcctaaa agcgattat ttggaaatt 
      ttatctaga gatttttcc agcgatagt ctagcgcac 
      gtattgaaa ccaatcaaa tttttgcaa cttgttaac 
      gaaccggaa gttgttaat ggcggagct aacgaggac 
      tgggttgtc gaaaaactt acttgcttc aaagaagaa 
      caacacagg gaaaaatta aacagacgg aaacttgta 
      actgttaaa cttagtttt gatacaaaa gttaagccg 
      acaaaagaa actttgtgt gaagacgct acgaagttg 
      aaaactcat ttccaggaa gccaatgaa aggcacttt 
      aagcgaaat ttgcgccaa ttgaattat aaaaattat 
      ttggttgtt gactacgat ttaaaacaa attgaagcc 
      gacttagaa tcgtccgca gatagttat cctaggcat 
      tacaaccct tatgaaagg ttttggatg cggtctcgg 
      atcggtatt gaggatatg agtcatgaa cagtgtcgt 
      gattttgtc aaaaaattt ccatataac tttcgttta 
      gttttcaag gaatgtcgg agaatagct gaacagaac 
      gatcaggag tttttggtc gaatacaat tttactgaa 
      ctttactta attcacatg gcaaatttt gggaaaaaa 
      aagcaaaca attataaaa aataaactc atcgctcac 
      tatctggat aaaaatgtt tcgttgtgg tga
    mRNA sequence
    • >TC010961 RA
      agactctcg tataattat aattgcgtg gttaattgg 
      ttaataata atttgcaaa gtttgtccg acataaccc 
      cacacttgc acgtgtgag ccctaaaat gcaggacat 
      agtgtgtga gcagtgcgg tggcgtcga tttctacaa 
      agaagctgg tttctactt ctgcagcgg gtgtcagat 
      tcagtctca ggaggtcca agagtacgt ttacgatga 
      atacacccc cgaaactcg cctaaagac taaaaaaat 
      ccaaagtga taagacagc gaagactga gaataaaat 
      aacaagttg ggagtgtta caactacat tcttcttgg 
      tcttacgaa tgaacttat aaatttggg ggctaataa 
      aaagttgaa ggcggtcgt tcgtctctt gtggatgcg 
      ttatttgga aaaattgca agttttcaa attggggcg 
      tgatgagtt accgaaatt gcaagccgt ctgtagcaa 
      catggacgc agaaattgt gtatggaaa actggcgaa 
      gaagaaacg aaagcgaaa caaatcgtc gtcaagcga 
      acttgacgt cacatcttt agattcaac ttctttgaa 
      gcatgaaag ggtcaagag aaagcgtgc tttagccaa 
      ggctcaata tgaggaatt cagtaaaaa gtctcaaga 
      gacaccttc actgcataa tgaaacttt gacaagtct 
      gcaaacagg ttctgagaa aagttctaa aaatgcagt 
      acctatcaa atacaatat ttatgctac taaagagtt 
      gagaaagat taaaagctc acaatacca ctggcgtaa 
      gcaccgaag ggactttga acgaaattt aaaatgtca 
      caagttgag ttatagaaa aatctcggg gaaataccg 
      ggctagccc ttacatact ttcaataaa caaacttta 
      cgctatttt atacctagg attgcttat tactaaaag 
      caaggtgca actgagcga cattttgag gttcattaa 
      agaggggca tttgagttt caattatta cagtcattt 
      ttttcccga agatttcgt cgataaaac actcaattt 
      tcgtaattt taagaaaaa cacaatgtt tgtgaatgc 
      accatttcg aacaattgc tgccgaaat gtcggtttt 
      attacaaat tgattttta tatttcgtg tcctaattt 
      aattgagtt gtgtgaacg ctattgtgg ggagttgaa 
      tttgcctcg tgagatttg cgattgcgc caaaaatat 
      tatcacaag ggctgatat taaaatgtc gtttcgtaa 
      acgaaatgg caaaatccc aaattacga aggtcgcgt 
      tttaagcgt tattctctt tgtgctcaa attattatt 
      tggattaga cgatgtcac agagtttca tttagcaaa 
      ctactcgaa tattttaaa taatagtga agctgttag 
      acctaaaat gtttaacat aatcgactg gcttaaata 
      catagaata tagggactt ggtgatgaa taatcagca 
      ctttcccac aagttttca cgacgagaa caatttcaa 
      caatgtaaa cttagcgtt aagtgcact tggaagaaa 
      agattattc agcatcagc aaaaccgaa ctcgacctc 
      agataacta caagcaact atttgaaaa attgaaaaa 
      ctatcttga cgtccctga ctttatcga atttccggt 
      tacgttcac tccgttttt ggattatgc taaacagtt 
      gcctaaaag cgattattt ggaaatttt atctagaga 
      tttttccag cgatagtct agcgcacgt attgaaacc 
      aatcaaatt tttgcaact tgttaacga accggaagt 
      tgttaatgg cggagctaa cgaggactg ggttgtcga 
      aaaacttac ttgcttcaa agaagaaca acacaggga 
      aaaattaaa cagacggaa acttgtaac tgttaaact 
      tagttttga tacaaaagt taagccgac aaaagaaac 
      tttgtgtga agacgctac gaagttgaa aactcattt 
      ccaggaagc caatgaaag gcactttaa gcgaaattt 
      gcgccaatt gaattataa aaattattt ggttgttga 
      ctacgattt aaaacaaat tgaagccga cttagaatc 
      gtccgcaga tagttatcc taggcatta caaccctta 
      tgaaaggtt ttggatgcg gtctcggat cggtattga 
      ggatatgag tcatgaaca gtgtcgtga ttttgtcaa 
      aaaatttcc atataactt tcgtttagt tttcaagga 
      atgtcggag aatagctga acagaacga tcaggagtt 
      tttggtcga atacaattt tactgaact ttacttaat 
      tcacatggc aaattttgg gaaaaaaaa gcaaacaat 
      tataaaaaa taaactcat cgctcacta tctggataa 
      aaatgtttc gttgtggtg attttcaat ttttgggat 
      gttttattt ttaataaat tacttcaca ttat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY