Status
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Gene information
General information
TC010961 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3427580..3433828
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC010961
    iBeetle screen iB_04951

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection

    larva lethality late (L7) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection

    larva lethality late (L7) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010961 RA
      MQDIVCEQC GGVDFYKEA GFYFCSGCQ IQSQEVQEY 
VYDEYTPET RLKTKKIQS DKTAKTENK ITSWECYNY 
ILLGLTNEL INLGANKKL KAVVRLLWM RYLEKLQVF 
KLGRDELPK LQAVCSNMD AEIVYGKLA KKKRKRNKS 
SSSELDVTS LDSTSLKHE RVKRKRALA KAQYEEFSK 
KSQETPSLH NETLTSLQT GSEKSSKNA VPIKYNIYA 
TKELRKIKS SQYHWRKHR RDFERNLKC HKLSYRKIS 
GKYRASPYI LSINKLYAI LYLGLLITK SKVQLSDIL 
RFIKEGHLS FNYYSHFFP EDFVDKTLN FRNFKKNTM 
FVNAPFRTI AAEMSVLLQ IDFYISCPN LIELCERYC 
GELNLPREI CDCAKNIIT RADIKMSFR KRNGKIPNY 
EGRVLSVIL FVLKLLFGL DDVTEFHLA NYSNILNNS 
EAVRPKMFN IIDWLKYIE YRDLVMNNQ HFPTSFHDE 
NNFNNVNLA LSALGRKDY SASAKPNST SDNYKQLFE 
KLKNYLDVP DFIEFPVTF TPFLDYAKQ LPKSDYLEI 
LSRDFSSDS LAHVLKPIK FLQLVNEPE VVNGGANED 
WVVEKLTCF KEEQHREKL NRRKLVTVK LSFDTKVKP 
TKETLCEDA TKLKTHFQE ANERHFKRN LRQLNYKNY 
LVVDYDLKQ IEADLESSA DSYPRHYNP YERFWMRSR 
IGIEDMSHE QCRDFVKKF PYNFRLVFK ECRRIAEQN 
DQEFLVEYN FTELYLIHM ANFGKKKQT IIKNKLIAH 
YLDKNVSLW
    CDS sequence
    • >TC010961 RA
      atgcaggac atagtgtgt gagcagtgc ggtggcgtc 
gatttctac aaagaagct ggtttctac ttctgcagc 
gggtgtcag attcagtct caggaggtc caagagtac 
gtttacgat gaatacacc cccgaaact cgcctaaag 
actaaaaaa atccaaagt gataagaca gcgaagact 
gagaataaa ataacaagt tgggagtgt tacaactac 
attcttctt ggtcttacg aatgaactt ataaatttg 
ggggctaat aaaaagttg aaggcggtc gttcgtctc 
ttgtggatg cgttatttg gaaaaattg caagttttc 
aaattgggg cgtgatgag ttaccgaaa ttgcaagcc 
gtctgtagc aacatggac gcagaaatt gtgtatgga 
aaactggcg aagaagaaa cgaaagcga aacaaatcg 
tcgtcaagc gaacttgac gtcacatct ttagattca 
acttctttg aagcatgaa agggtcaag agaaagcgt 
gctttagcc aaggctcaa tatgaggaa ttcagtaaa 
aagtctcaa gagacacct tcactgcat aatgaaact 
ttgacaagt ctgcaaaca ggttctgag aaaagttct 
aaaaatgca gtacctatc aaatacaat atttatgct 
actaaagag ttgagaaag attaaaagc tcacaatac 
cactggcgt aagcaccga agggacttt gaacgaaat 
ttaaaatgt cacaagttg agttataga aaaatctcg 
gggaaatac cgggctagc ccttacata ctttcaata 
aacaaactt tacgctatt ttataccta ggattgctt 
attactaaa agcaaggtg caactgagc gacattttg 
aggttcatt aaagagggg catttgagt ttcaattat 
tacagtcat ttttttccc gaagatttc gtcgataaa 
acactcaat tttcgtaat tttaagaaa aacacaatg 
tttgtgaat gcaccattt cgaacaatt gctgccgaa 
atgtcggtt ttattacaa attgatttt tatatttcg 
tgtcctaat ttaattgag ttgtgtgaa cgctattgt 
ggggagttg aatttgcct cgtgagatt tgcgattgc 
gccaaaaat attatcaca agggctgat attaaaatg 
tcgtttcgt aaacgaaat ggcaaaatc ccaaattac 
gaaggtcgc gttttaagc gttattctc tttgtgctc 
aaattatta tttggatta gacgatgtc acagagttt 
catttagca aactactcg aatatttta aataatagt 
gaagctgtt agacctaaa atgtttaac ataatcgac 
tggcttaaa tacatagaa tatagggac ttggtgatg 
aataatcag cactttccc acaagtttt cacgacgag 
aacaatttc aacaatgta aacttagcg ttaagtgca 
cttggaaga aaagattat tcagcatca gcaaaaccg 
aactcgacc tcagataac tacaagcaa ctatttgaa 
aaattgaaa aactatctt gacgtccct gactttatc 
gaatttccg gttacgttc actccgttt ttggattat 
gctaaacag ttgcctaaa agcgattat ttggaaatt 
ttatctaga gatttttcc agcgatagt ctagcgcac 
gtattgaaa ccaatcaaa tttttgcaa cttgttaac 
gaaccggaa gttgttaat ggcggagct aacgaggac 
tgggttgtc gaaaaactt acttgcttc aaagaagaa 
caacacagg gaaaaatta aacagacgg aaacttgta 
actgttaaa cttagtttt gatacaaaa gttaagccg 
acaaaagaa actttgtgt gaagacgct acgaagttg 
aaaactcat ttccaggaa gccaatgaa aggcacttt 
aagcgaaat ttgcgccaa ttgaattat aaaaattat 
ttggttgtt gactacgat ttaaaacaa attgaagcc 
gacttagaa tcgtccgca gatagttat cctaggcat 
tacaaccct tatgaaagg ttttggatg cggtctcgg 
atcggtatt gaggatatg agtcatgaa cagtgtcgt 
gattttgtc aaaaaattt ccatataac tttcgttta 
gttttcaag gaatgtcgg agaatagct gaacagaac 
gatcaggag tttttggtc gaatacaat tttactgaa 
ctttactta attcacatg gcaaatttt gggaaaaaa 
aagcaaaca attataaaa aataaactc atcgctcac 
tatctggat aaaaatgtt tcgttgtgg tga
    mRNA sequence
    • >TC010961 RA
      agactctcg tataattat aattgcgtg gttaattgg 
ttaataata atttgcaaa gtttgtccg acataaccc 
cacacttgc acgtgtgag ccctaaaat gcaggacat 
agtgtgtga gcagtgcgg tggcgtcga tttctacaa 
agaagctgg tttctactt ctgcagcgg gtgtcagat 
tcagtctca ggaggtcca agagtacgt ttacgatga 
atacacccc cgaaactcg cctaaagac taaaaaaat 
ccaaagtga taagacagc gaagactga gaataaaat 
aacaagttg ggagtgtta caactacat tcttcttgg 
tcttacgaa tgaacttat aaatttggg ggctaataa 
aaagttgaa ggcggtcgt tcgtctctt gtggatgcg 
ttatttgga aaaattgca agttttcaa attggggcg 
tgatgagtt accgaaatt gcaagccgt ctgtagcaa 
catggacgc agaaattgt gtatggaaa actggcgaa 
gaagaaacg aaagcgaaa caaatcgtc gtcaagcga 
acttgacgt cacatcttt agattcaac ttctttgaa 
gcatgaaag ggtcaagag aaagcgtgc tttagccaa 
ggctcaata tgaggaatt cagtaaaaa gtctcaaga 
gacaccttc actgcataa tgaaacttt gacaagtct 
gcaaacagg ttctgagaa aagttctaa aaatgcagt 
acctatcaa atacaatat ttatgctac taaagagtt 
gagaaagat taaaagctc acaatacca ctggcgtaa 
gcaccgaag ggactttga acgaaattt aaaatgtca 
caagttgag ttatagaaa aatctcggg gaaataccg 
ggctagccc ttacatact ttcaataaa caaacttta 
cgctatttt atacctagg attgcttat tactaaaag 
caaggtgca actgagcga cattttgag gttcattaa 
agaggggca tttgagttt caattatta cagtcattt 
ttttcccga agatttcgt cgataaaac actcaattt 
tcgtaattt taagaaaaa cacaatgtt tgtgaatgc 
accatttcg aacaattgc tgccgaaat gtcggtttt 
attacaaat tgattttta tatttcgtg tcctaattt 
aattgagtt gtgtgaacg ctattgtgg ggagttgaa 
tttgcctcg tgagatttg cgattgcgc caaaaatat 
tatcacaag ggctgatat taaaatgtc gtttcgtaa 
acgaaatgg caaaatccc aaattacga aggtcgcgt 
tttaagcgt tattctctt tgtgctcaa attattatt 
tggattaga cgatgtcac agagtttca tttagcaaa 
ctactcgaa tattttaaa taatagtga agctgttag 
acctaaaat gtttaacat aatcgactg gcttaaata 
catagaata tagggactt ggtgatgaa taatcagca 
ctttcccac aagttttca cgacgagaa caatttcaa 
caatgtaaa cttagcgtt aagtgcact tggaagaaa 
agattattc agcatcagc aaaaccgaa ctcgacctc 
agataacta caagcaact atttgaaaa attgaaaaa 
ctatcttga cgtccctga ctttatcga atttccggt 
tacgttcac tccgttttt ggattatgc taaacagtt 
gcctaaaag cgattattt ggaaatttt atctagaga 
tttttccag cgatagtct agcgcacgt attgaaacc 
aatcaaatt tttgcaact tgttaacga accggaagt 
tgttaatgg cggagctaa cgaggactg ggttgtcga 
aaaacttac ttgcttcaa agaagaaca acacaggga 
aaaattaaa cagacggaa acttgtaac tgttaaact 
tagttttga tacaaaagt taagccgac aaaagaaac 
tttgtgtga agacgctac gaagttgaa aactcattt 
ccaggaagc caatgaaag gcactttaa gcgaaattt 
gcgccaatt gaattataa aaattattt ggttgttga 
ctacgattt aaaacaaat tgaagccga cttagaatc 
gtccgcaga tagttatcc taggcatta caaccctta 
tgaaaggtt ttggatgcg gtctcggat cggtattga 
ggatatgag tcatgaaca gtgtcgtga ttttgtcaa 
aaaatttcc atataactt tcgtttagt tttcaagga 
atgtcggag aatagctga acagaacga tcaggagtt 
tttggtcga atacaattt tactgaact ttacttaat 
tcacatggc aaattttgg gaaaaaaaa gcaaacaat 
tataaaaaa taaactcat cgctcacta tctggataa 
aaatgtttc gttgtggtg attttcaat ttttgggat 
gttttattt ttaataaat tacttcaca ttat
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