| General information | |||
| TC010960 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 3439520..3460588 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC010960 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04950 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Days post injection
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 20
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
- >TC010960 RA
MQPKTQNIP ILEEIITTT LSEDLLERF NISSKKRSY LREYKCKIS KETKKKEHK TKLKELYPE VPQKNTSHF DIRAFAEAG PKTIKLMFV SREIMRRVQ IGGLQNVFP QVVETILHE ARDEFIETI HGVSVNLKT KPLDHTDGH IKLESFKFL GKSKRYDVF LTNRSKMLK KWKLNHPLI RAILNECVT FLPDKIFDF GAKNNEPID IAVLNDIFH NYTKEAKTL FKDLYTRLF NLVQKDKSK GRDHLPACT GLFSVHLSQ TIIETMKYI TRVTGDEDL IPYLKLTLI FGQEGLSLK PTMEEVIGL YRRFFNELV ATGEKFPVL ESAKNEDLE EKIMYLNVT EDFIDSCVD QMASNIAKM YEPIFAYLD YVESEFGDV YADFGDEEE MTFTKGFNQ IAYYKSYIK KAMTNLTNE YFGIGQLIL SEYVDNLKA SLQQIIDDT HAKISLEHR REDEAICQC FQDIQEKAL EIPANTEEL IAQGKHMIW VKTVQLKEL RDRIQMMLH NLVKLIDFG EITRDHMDL NSDTVNWLT LIEPVLEHN STMYEQFKF EAEEKLQKT KEEIVFALT ELEPKLVIL NEMDDVINV RQYVRQMAP FLEVMRDVR TKIIWSNKE ETSLGFGVT TFNKYEEVA NFLFPFYHL LKLCLNLER KIGVWFDGQ FEFLDAEET EAQIEEFQK ELLKIQKTY RIKLRQAQA ENISLRFDG IVDDPELVN WPAPLKLTA KAIQNLKDF RPALTMMKI MCNDALLKR HWKEMSEVA GFDMTPNAG TSLRKLMMM GLEADIDKY EIISSSATK ERELLKNLN KMQAEWVDI CFKTGIYKD TGIHILTQL DDIQVVLDD HILKTLTMR GSVFVRPYE TEVKSFYEK LTRINSTIE EWGKVQSQW LYLLPIFSS KDIVSQMPE EGLLFKEVN DTYKRYMDM VLRDPRVTE TAGAMGVLE AMVHCIELL EKINDGVVS YLERKRLFF PRFFFLSND EMLEILSET KDPLRVQPH LKKCFEAIN RLNFDEQLQ IHAMYSQEG EQIDFVEMI KTKEFGGSV EKWLILVED QMVKSVREQ IIKSWKNYF VTPRTKWVQ KWPGQVVLA VSQMHWTAH VHKALNRVE DMSIESFSE QLKKQLQEI VGLIRDPTL TNLSRITIK ALIVIDVHA KDVVHELHE KRVKDDKEF KWLSQMRYY LEDDESYVR LINATVRYA YEYLGNTDR LVITPLTDR CYRTLIGAY HLHLNGAPE GPAGTGKTE TTKDLAKAI AVQCVVFNC SDGLDYKAM GKFFKGLAS CGAWACFDE FNRIDIEVL SVVAQQILS IIMAVRANA SKFIFEGTE ISLNPACYV CITMNPGYA GRTELPDNL KVLFRTVAM MVPDYAMIG EISLYSYGF IDARNLSVK IVTTYRLCS EQLSSQNHY DYGMRAVKS VLSAAGNNK RNFPDEHED ILLLRAILD VNLPKFLNQ DLPLFEGII SDLFPGVVL PEADYKMLT NAMNISCQK RNLQPKSSF ILKIIQTYE MMIVRWGFM IVGEPFAGK TSTLKILAD TLTLINSQG LGESNVQYT ILNPKAITM GQLYGQFDP ISYEWSDGV VATCFRNYA HDSSPDRKW IIFDGPVDA VWIENMNSV LDDNKKLCL MSGEVMTLS STMSLIFEV MDLEQASPA TVSRCGMIY MEAVTLGWE PFMESWIVN CNPDWCHDE FKTLIIDIF KWIVPPCLY FIRKQCVQY CNIGNINLV KNMMQLFEM TLNKALSET EDTKNLLIW MQAALIQAG LWGLGCVLD SDSKIKFDT FYKQLWRNQ IEECPYPAS MEKLELNIP IEGLLYDYS YYFRMKGTW KYWPEVVRN ERVEECANI LQAMVPTVD TARYMALVD LHIKNNLPV LLVGPTGTG KSFYIQDLL MNQLDKELY LPSFITFTV KITANQTQN LIISKLNKL RRSHYGAPK GKTCVLFID DINMPYKET YGAQPPIEL LRQYIDHKN WYDLKTTEP IYLHNVIFL AAMGLVGGS RQEIYPRFL RHFSIFSIN EFSEETMAK IYTNILHLG WKNNGFPSD VISLAVQTV GASLDMYKS AMDNLRPTP SKSHYVFNL RDFSRLIQG CAMLRKECV ESKVIFAKI WVHEVLRVF YDRLVEETD KNWLFAKLK LVVRDHFKE HFDTIFDNL PKDSDGVLT LNSLKKLMF GTYFDQDSN DDERRYEET INIKQFADV CQLCLDEYN STHKTKMDV VLFDYALEH LSKICRVLS MACGSCLLV GISGSGRQS LTRLASEIY SQHLYQPEI TNNYGLNEW RDDIKKILK EGGGRGRHC VFLISEGQI KEESFLQDI DCLLNSGEV PNIYQIDER QEILDMVRL AAQGGNRNL DISALQIFY FFTKRCREK LHIILCFSP VGSTFRNRL RLYPSLINC CTIDWFDDW PENALEEVA FKWMSDVNI PQEVKNQAV IACKYFHVE ARLVSDEFY KVSGRKTYI TSASYLELI KSFTILTNA KQLEILTTQ KRYIGGLKK LLFAAEQIA EMQKNLANL QPQLEEMNQ KAIDMMKQI ESETIEVEK ASELVKKDE KVANIQAAM ASALKRECE ADLAEAIPI LEDAIRALD TLKPADITL VKSMKNPPA AIKLVMAAV CIMKDVKPA RIRDPSTGR MNLDFWGPS VKVLGDMNF LQSLKDYDK DHIKPEIMV KIRREFLPH KDFKPAVVA KASSAAEGL CKWIIAIDK YDEVNKIVA PKKEKLDKA EREFAATME ILRAKREQV KQLEEKLAI LNEQLGEAV QKQGELQAD VDLCKNKLI RAQKLIGGL GGEKTRWTA AANSLQEQH DSLAGDILI SSGIMAYLS PLTAFYRQK TVADWHKIV KELKIPCSD TFDFLFVLG SDVKVQNWY ISGLPRDSF STENGIIAD NSRKWSLFI DPQYQASNW IKKMEKKNS LYVVKFSHS DYMKKIENC VQNGYPVLI ESIGEVLEA PIDPLLYKK VFKQAGFEV ISMGENVIP YNRNFRLYL ASPLRNPHY LPEVFNRVT IINFALTLE GLQDQLLGI VVAVEKPEL QRLKEELIV QKAENKAAL DNVELNILR TLSECKGDI LEDESAIQI LDESKLLSI TIREKQSKS LEIEKTIEE FRVLYQGVA EHSAVLYYC ICDLGNVDP MYQYSLDWF INLYIGSIQ RAEKCKSVE KRCTNLITA FTFDLYSNI TRSLFEKDK LLFSFLLCS KILIFRKAL DEKEFMFLL TGGVNVENP VPNPVNWLP NQAWDEICR VDELPAFHN FRDSFVQAS RDWKRIYDS YEPEKERLP GPWHDKLNT FRKLIVIRI LRSDKLIVG ATDYIAKEM GVKYITPPQ FNISISYNE SYNLCPLIF ILSPGTDPM SALVKFAEE KKYTEKFQS ISLGQGQGP RAAALIQQG QDEGLWVCL QNCHLATSW MPSLEKIFE DMDYNNTND NFRLWLTSY PSDKFPVSI LQKGVKITN EPPTGLKDN LLKSYVNDP VKNQDFYNG CPGNYEMFV KLLYGIAFF HAVVQERRT FGPLGWNIP YGFNDSDFD ISVQQLQMF INESENPYE ALSYLIGEC NYGGRVTDD WDRRLITTI LEDFLNPRV ATSNNYLFS DAADCYGLP IKIDYQDFV THIQNLPHV HPPQVFGLN TNAGITRDM QNSQRLLNS VLKAYGEIQ TGGVGETDK YIMMLCSDI LSKLPKPFD QEEAIRRYP VQYSESMNT VLVQEMDRF NRLLSTIRN SLINMQKAI QGLVSMTPS LESFASSLL IARIPSNWA SSSYPSLKN LPHYVADFL SRIEFLETW FKRGKPDDY WISGFFFTQ AFLTGVKQN YARKYTIPI DKLTFDFEI LQKDRGDRA PKDGAYIYG LFTDGARWD RARGQIDEL LPKVLHDNM PLIWIKPIK DKDYKERGR YRCPVYKTS ERRGVLSTT GHSTNYVLP ILMETSVKP AHWIKRSVA LLCQLD
- >TC010960 RA
atgcaaccc aaaactcaa aacatccca attttagaa gaaataata accacgaca ctatccgaa gatttgcta gaacgtttc aatatctcc tcaaaaaag cgtagttat ttacgtgaa tacaaatgc aaaatatca aaagaaacg aaaaagaaa gaacacaag acgaaactg aaagaacta taccctgaa gtaccccaa aaaaacacg tcacacttt gatatccgg gcatttgca gaagcgggc cccaaaaca ataaaactg atgtttgtc tcacgcgaa ataatgaga cgagttcaa atcggaggc ctccaaaac gtgtttcct caagtagtt gagacgatt ttacacgaa gcaagagac gaatttatt gaaacaatc catggagtg agtgttaat ttaaaaacg aaacctttg gatcacact gatggacac ataaagctt gagtcgttc aaatttttg ggaaaatcg aaacgctac gatgttttt ttgaccaac agaagtaaa atgttaaag aagtggaaa ctaaatcat cccctaatc cgcgcaatt ctcaacgaa tgtgtcacg tttctaccg gacaagatt ttcgatttt ggggctaaa aacaacgaa ccgattgat atcgcagtg ttgaacgat attttccac aattacacc aaagaagct aaaaccttg ttcaaagat ttgtacaca agactcttc aatttggta cagaaagac aaaagcaaa ggtcgtgat catttgcca gcttgcact gggcttttt tcggtccac ttaagtcaa acaatcatc gaaaccatg aaatatatc actagagta acaggggac gaagacttg attccgtac ctcaaactc actttaatt tttggacaa gaaggactt tccttgaaa ccaacaatg gaagaagtg attgggcta tacagacga tttttcaac gaacttgtt gcaactggt gaaaaattt cccgtgctt gaatcggca aagaatgaa gatttggaa gagaaaatt atgtatctt aatgtaact gaagatttt attgattca tgtgttgac caaatggcc agcaatata gccaagatg tatgaaccc atttttgca tatcttgat tacgttgag tctgaattt ggtgatgtg tatgctgat tttggcgac gaagaagaa atgactttc accaaaggt ttcaaccaa attgcttac tacaaaagc tacatcaaa aaagcaatg actaaccta accaatgaa tattttggc attggtcaa ctcatcttg tcggaatac gttgataat ttgaaagct tcccttcaa caaatcatt gatgatacc cacgccaaa atcagtctg gaacataga cgagaagac gaagcaatt tgtcaatgt tttcaagac attcaagaa aaggcactt gaaattcca gccaatacc gaagaactc atcgcccaa ggtaaacac atgatttgg gtcaagaca gttcaactc aaagaacta agagatcgc atccaaatg atgctacac aatttggtg aaactgatc gactttggt gaaatcaca cgcgaccac atggacttg aactccgac actgtcaac tggttgacc ttgatcgaa ccagtcctt gaacacaac tccaccatg tacgaacaa ttcaaattc gaagcggag gaaaagctc cagaaaacg aaagaagaa attgttttc gcgctcact gaactggaa cccaaactt gtgattctg aacgaaatg gacgatgta atcaatgtg agacaatac gtacgtcaa atggcccca tttctcgaa gtgatgcga gacgtccga accaaaata atctggtcc aacaaggaa gagacaagt ttggggttc ggggttacg actttcaac aaatacgaa gaagtggca aactttctg ttcccgttt taccacttg ttgaagctt tgcctaaac ttggaacgc aaaattgga gtttggttc gacggccag tttgagttt ctcgatgcc gaggagacc gaagcccaa attgaggag ttccagaag gagttgctc aaaatacaa aaaacctat cggattaag ttgcgccaa gcacaagct gagaatatt tcgctgcgt tttgatgga attgttgat gatcctgag ctcgtgaac tggcccgct ccgctaaaa ctcaccgcg aaagcaatc cagaatttg aaggacttt cgacctgcg ttaaccatg atgaagatt atgtgtaac gatgctttg ttgaagcga cactggaag gagatgtcc gaagttgca ggtttcgac atgactcca aatgctgga acttctctg cgaaagctt atgatgatg gggcttgaa gctgatatt gacaagtac gaaattatc agttctagt gccaccaaa gaacgcgaa ctgttgaaa aatctgaat aaaatgcag gcggagtgg gttgatatt tgtttcaag actggaatt tacaaggat acggggatc catatcttg actcagttg gatgatatt caagttgtg ctagacgac cacattttg aagactttg acgatgcga ggatcagtc tttgtgaga ccttacgaa accgaggtt aagagcttt tacgagaag ttgactcga atcaacagt acgattgaa gagtggggg aaggtgcaa tcacaatgg ttgtacttg ctgccgatt ttctcgtcc aaagacatt gtgagccaa atgcctgaa gaaggccta cttttcaaa gaagtgaac gatacgtac aagcgttac atggacatg gtcttgagg gaccctcga gtcacggaa accgctggt gcgatgggt gttttggaa gcaatggtt cattgtatc gagcttttg gagaaaatt aacgatggc gtagtttcg tatttggaa cgcaaacga ttatttttc ccacgtttc ttcttccta tccaacgac gagatgttg gagatttta tccgaaacg aaggaccca ctccgggtg caaccccac ttgaagaag tgttttgaa gcgatcaat cggttaaat ttcgacgaa caattgcaa atccatgcc atgtacagt caagaaggg gaacaaatc gactttgtc gaaatgatc aaaacgaag gaatttggt ggtagcgtc gaaaagtgg ttgattttg gtcgaagac cagatggtt aagtcagtt cgtgagcaa ataatcaaa agttggaag aattatttt gtaacaccg cgaactaaa tgggtccag aaatggcca ggtcaggtg gtcttagcc gtgtcacaa atgcactgg actgcgcat gttcataaa gcgttgaat cgggtcgaa gacatgtcg attgagtcg ttttctgag caactcaag aaacaactc caggaaata gttgggttg attcgtgat ccaactctc acaaactta tcgcggatc acaatcaaa gccttgatt gtgattgat gttcatgcg aaagatgtt gttcacgaa cttcacgaa aaacgtgtc aaggacgat aaggagttt aagtggttg tcacaaatg aggtactac cttgaagac gacgagtct tacgtccga ttgattaat gccacagtt cggtatgcg tatgaatat ttgggaaat actgatcgt cttgtgatc actcctttg actgatcgg tgttatcga acgttaatc ggagcatat catttgcac ttaaatgga gctcctgaa gggccggct gggactggg aaaactgaa actaccaag gatttggcc aaggcaata gctgtacag tgtgtggtg tttaattgt tcagatggg ttggactat aaagctatg gggaagttt ttcaaaggt ttggcatcg tgtggtgct tgggcatgc ttcgacgaa ttcaaccga attgatatt gaggtttta tcagtggtt gcccaacaa atcctctcc ataatcatg gcagttcgt gctaatgca tcgaaattt attttcgaa ggaaccgaa atatcccta aatccagct tgttacgtt tgcatcacc atgaatcct ggctatgcc ggccgaacc gaactacca gacaattta aaagttctg tttcgaaca gttgcaatg atggtacca gattatgca atgattggt gaaatttcc ttgtattcg tatggtttt atcgacgct cggaacctt tcagtcaag atcgtcacg acttatcgg ctatgttcg gaacaacta tcgtcccaa aaccactac gattatggt atgagagcc gtcaaatcc gtgctttcg gcagctggg aataacaaa cgaaatttc ccagacgaa catgaggat attttgctc ttgagggcg atacttgat gttaattta cccaaattt cttaatcaa gaccttcca cttttcgaa ggtattatc tctgatttg ttcccaggg gttgtactc cccgaagct gattacaaa atgttaaca aacgccatg aatatcagt tgccagaaa cgcaatctt caaccgaaa agttccttc attttaaag atcattcaa acgtacgaa atgatgatc gttaggtgg ggttttatg attgttggg gagcctttc gctgggaaa acttcaact ttgaaaatt ttggccgat actttaaca ttgatcaat agtcaaggg ttgggcgaa tcaaacgtt caatatacg attttgaat ccgaaagcg attacaatg ggacaactc tatggtcag tttgatccg atttcgtac gaatggtcg gatggtgtt gttgcgact tgttttcgg aattatgcc catgattcg agtcctgac aggaaatgg attattttt gatggacct gttgatgcc gtctggatt gaaaatatg aacagtgtg ttggatgat aataagaaa ctttgtttg atgagtggg gaagttatg accttgtcg agtaccatg tcgttgatt tttgaagtc atggatttg gagcaagct tcaccagcg actgtatct cgctgtgga atgatttac atggaagct gtaacactc ggctgggaa cccttcatg gaatcatgg attgtaaat tgtaaccct gactggtgc cacgatgaa tttaaaact ctaatcatc gatatcttc aaatggatt gtaccccca tgtctttat ttcattcga aaacaatgt gtgcaatac tgcaacatt ggaaacatc aacttggtg aaaaacatg atgcaacta tttgaaatg actctaaac aaggcttta tccgagacg gaagacaca aaaaattta ctaatctgg atgcaggca gcattgatt caggccggt ttatgggga cttggttgt gtccttgat tcagactca aagatcaaa tttgacacg ttttacaag cagttgtgg cggaatcaa attgaggaa tgtccatat ccagcttcg atggaaaaa cttgagttg aatatacca atcgaaggg ttactttac gactattcc tattatttt cgaatgaaa ggtacttgg aagtactgg ccggaagtg gtacgtaac gaacgagtc gaagagtgc gcaaatatt ttacaagca atggttcca acagttgat accgcacgt tatatggct ttggtcgac cttcacatc aaaaataac cttccagtt ttattggtt gggccaaca gggactggc aaaagtttc tatatccaa gatctgcta atgaatcaa ttggacaag gaactgtac ctaccttcg tttatcaca ttcaccgtg aaaatcaca gcgaatcaa acccaaaat ttgatcatt tccaagcta aacaaattg aggcgttca cattatgga gcccctaaa ggcaaaacc tgtgttctc ttcattgac gatatcaac atgccttat aaggaaaca tacggtgca caaccgccc atcgagtta ctccgccaa tacattgat cacaagaac tggtacgac cttaaaacc accgaaccg atttattta cacaacgtt attttcctc gctgccatg ggtctggtt ggtggtagc cgccaagaa atctatcct cgttttttg cgccatttc agcatattc tcgattaat gagttttcg gaagaaacg atggccaaa atctatacc aacatccta catttgggg tggaaaaat aacggtttt ccgagcgat gtaattagc ttagctgta caaacagtt ggtgctagt ttagacatg tacaaatca gcgatggat aatctaaga cctacaccg tcgaagagt cactacgtg ttcaatctg cgtgatttc tcacgtttg attcaaggt tgcgccatg ctacgtaaa gagtgtgtg gaaagtaag gttattttt gcgaaaatt tgggttcac gaagtgtta agggttttc tacgaccgt ttggtagaa gaaaccgac aagaattgg ttgtttgcg aagttaaaa ctagtagtg cgtgaccat ttcaaggaa cattttgat acaatcttt gataatcta ccgaaagac tctgacggg gttttgaca ctcaattcc ttgaagaaa ctgatgttt gggacttac tttgaccag gattcaaac gacgatgaa cgaaggtat gaggaaacg atcaatatc aagcaattt gccgatgtg tgccaactg tgcttggat gagtacaat tcaactcac aagacaaaa atggatgtt gtgttattc gactatgcc ctcgaacac ttgtccaaa atttgtcgt gtcttgtca atggcttgc ggtagttgc ttattggtt ggtatcagt ggttccggt cgtcagtcc ttaacccga ctagcaagt gaaatttat agtcaacac ttgtaccaa cctgaaatc actaataac tacggtttg aacgaatgg cgtgacgat attaagaaa atcttaaag gaaggaggt ggacgcggt cgccattgt gtcttcctg atttccgaa ggtcaaatc aaggaagaa tcgttcttg caagacatt gattgtttg ttaaattcg ggcgaagtc ccgaacatt tatcaaatc gatgaacga caagaaatt ttggatatg gtgcgtctg gcggcgcaa ggagggaac cgaaacttg gatatttcc gcccttcaa atcttttat ttctttacg aaacgttgc cgcgagaag ttacacatt attttgtgc ttcagtccg gttggttca accttccgc aatcgtttg aggctttac ccgtccttg attaattgt tgtacgatt gattggttt gacgattgg cccgaaaat gccttagaa gaagttgcg tttaaatgg atgtcggat gttaacatc ccccaggag gtgaagaac caagctgtg atcgcgtgc aagtatttc cacgtcgag gcaaggctt gtttccgat gaattttat aaagtatca ggtcgcaaa acttacatc acctcagct tcgtatctc gagttgatc aaatcgttc acaatcctg actaatgcg aaacagttg gagatttta acaactcag aaacgttac attggtggt ttgaagaaa ctactcttc gcggcggaa caaatcgcc gaaatgcaa aaaaacctg gcgaacttg caaccacaa cttgaagaa atgaaccaa aaggcgatt gatatgatg aaacaaatc gaaagtgag actattgag gtggagaaa gcttccgag ttggtgaaa aaggacgag aaagttgca aatattcaa gctgcaatg gcttcggcc ttgaaacgt gagtgtgaa gctgatttg gccgaagct ataccaatt cttgaggac gcgatacgt gccttggac acactcaaa ccagccgat atcactttg gtcaaatca atgaaaaac ccaccagct gcaatcaaa cttgtgatg gctgctgtt tgcataatg aaagatgtt aagccggca cgtattcgt gacccttcg actggtcga atgaacttg gatttttgg ggtccgagt gtgaaagtt ttgggcgat atgaacttc ctccaaagt ctcaaagac tacgacaag gaccacatc aagcccgaa attatggtt aaaatacgt agagagttt ctcccccac aaggatttt aaaccagca gttgtggcg aaggcatcg agtgctgcc gaaggtctc tgtaagtgg attatagcc attgataag tacgacgag gtcaataaa attgttgca cctaaaaaa gaaaagctg gataaggcg gaacgcgag ttcgctgcc actatggaa atactacgg gcgaagcgc gaacaagtc aaacagttg gaagagaaa ttggcgatt ttgaacgaa cagttgggc gaagccgtc cagaaacaa ggcgaactt caagccgat gtggacttg tgcaaaaat aaattaata cgggcccaa aaattgatc ggtggactt ggtggtgag aaaaccagg tggacagct gcagccaat tcacttcaa gaacaacac gattcattg gctggcgat attttgatt tccagtggc attatggcg tatttatca cccttgact gcgttctac cgccaaaaa actgtagcc gactggcac aaaatcgtc aaagagttg aagattcca tgttccgac acgtttgat ttcctgttt gttttgggt tcggatgtt aaggtacaa aactggtac atcagtggg ctaccacgc gatagtttc tcgactgaa aatggcata atagcggac aattcgcgg aaatggtcc cttttcatt gatccccaa taccaggcc agtaactgg atcaaaaaa atggagaag aagaattcg ctctacgtt gttaaattc tcccactcc gactatatg aaaaaaatc gaaaattgc gtccagaac ggatatccc gtcctgatc gaaagcatt ggggaggtt ttggaagca ccaattgat cctctcctg tacaaaaaa gtgttcaaa caagctggg ttcgaggtg atcagtatg ggggaaaac gtaatccct tacaatcga aactttaga ttgtatctt gcatcaccc ttgaggaac ccacactat ttacccgaa gttttcaat cgtgtgaca attatcaac tttgcccta actctcgaa gggctccaa gaccagtta ctggggatt gtggtcgca gtggaaaaa cccgaattg cagcgactc aaagaagaa ctgattgtg caaaaagcg gaaaacaag gctgcgcta gacaacgtc gagttgaac attttgcga acgctttcg gaatgtaaa ggggatatc cttgaggat gaatccgcg atccaaatt ctagacgaa tcaaaattg ttgtccatc acaattcgc gagaaacag agcaagtct ttggaaatt gaaaagaca atcgaggag tttcgcgtt ttgtaccaa ggggtagcc gaacattcc gccgttctc tactattgc atttgtgac ttggggaac gttgaccca atgtatcaa tactccctc gactggttt ataaactta tacattggt tcaatccaa cgagccgag aaatgtaaa agtgtggag aaacgttgc accaattta ataacagcc ttcacgttt gatctctac tccaatatc acacgttcc ctcttcgag aaggataag ttattgttt tccttcttg ctttgctcc aagatactg atttttcgc aaagcgctc gatgagaag gagttcatg tttcttttg acgggtggt gttaatgtt gaaaatccg gtcccaaac cccgtcaat tggttacca aaccaggcg tgggacgag atatgtcga gtggatgaa ttaccggcg tttcacaat tttcgcgat tctttcgtt caagcgagt cgcgactgg aagaggatt tacgatagc tacgaaccg gagaaggaa agattgcca gggccttgg cacgacaaa cttaacacg tttcgaaag ttgattgtg attcgcatt ctgcggtcc gataagttg atagtgggt gccactgat tatattgca aaggagatg ggggtgaag tatatcacg ccgcctcag tttaacatt tccatttcg tacaatgag tcttacaat ttgtgtccg ttgattttt atattgtca cccgggact gatccaatg tccgcgcta gtgaaattc gcagaagag aagaaatat acggagaag tttcaatca atttctttg gggcaaggt caggggcca cgtgccgca gctttgatc cagcaaggt caagacgaa ggattatgg gtgtgtctg caaaattgt cacttggcc acgtcttgg atgccaagt cttgaaaaa atcttcgaa gatatggat tataataac actaatgat aacttcagg ttgtggcta acgagttac ccgtctgat aagttccca gtgtcaata ctccagaaa ggagtgaaa atcaccaac gaaccaccg actggtttg aaagacaac ttgttgaaa tcttacgtt aacgatccg gtgaagaac caagatttt tacaacggt tgtcccggg aattacgag atgtttgtc aaactgttg tatggcatt gcgtttttt cacgccgtt gtgcaagaa cgccgaact tttggccca cttggttgg aatattccg tacggtttt aacgattcg gattttgat atcagtgtc caacaattg caaatgttc atcaatgag agtgaaaat ccgtatgaa gcattatcg tacctgatt ggagagtgt aattacgga ggtcgtgtc acagatgat tgggatcgt cgattgatt actacaatt ctagaggat tttcttaat cctcgagtc gcaacttcg aacaattat ctttttagt gacgcggcg gattgttat ggccttccg attaaaatc gactatcaa gattttgtc acccacatc caaaattta ccacatgtg caccctccg caagtgttt ggacttaac acaaatgct ggaataacg cgcgatatg caaaactcg caacgcttg ttaaattcc gtccttaaa gcttacggt gaaattcaa actggtggt gttggtgaa accgacaag tacatcatg atgttgtgt tccgatatc ttatccaaa ttaccaaaa ccgttcgat caggaagaa gcaattcgt cgttacccc gtccagtat tcggaatca atgaacact gtgttggtg caagaaatg gatcgtttc aaccgactg ctttccacc atccgcaac tctctaatc aacatgcag aaagcgata caaggactg gtttcaatg accccttcc cttgaatcc ttcgcctct tctctgcta atcgctcga atcccttca aactgggcg agcagttcg tatccaagc ctcaaaaac ttgccccat tacgtggcc gattttttg tcaaggatt gagtttctc gaaacttgg ttcaagcgt ggtaaaccg gatgactat tggatttct gggtttttc ttcactcag gcgtttcta accggtgtt aagcagaat tacgcaagg aagtataca attccgatt gataagctt acgtttgat tttgagatt ttgcaaaaa gatcgtgga gatagagca ccaaaagat ggggcgtat atttatggg ttgtttact gatggggcg agatgggat cgtgccaga ggacaaatt gatgagttg ctgccaaag gtgttgcat gataatatg cccttgatt tggattaaa ccgatcaaa gataaggac tataaagag agaggaagg tataggtgt ccggtttat aaaacttca gagaggaga ggggtgttg tcgacaacg gggcactca acgaattat gtgctgccg atcttgatg gaaacaagt gtgaaaccg gcgcattgg attaagagg agtgtggca ttgctctgt cagttggat taa
- >TC010960 RA
gcgcaccaa ctcagtgga gacaagtga taatccttc ttttaaaac aaaaaataa tttgataaa cccttcaaa tggacaata aatacaaag aaatttttc gaagatgtc gaaaaatgc aacccaaaa ctcaaaaca tcccaattt tagaagaaa taataacca cgacactat ccgaagatt tgctagaac gtttcaata tctcctcaa aaaagcgta gttatttac gtgaataca aatgcaaaa tatcaaaag aaacgaaaa agaaagaac acaagacga aactgaaag aactatacc ctgaagtac cccaaaaaa acacgtcac actttgata tccgggcat ttgcagaag cgggcccca aaacaataa aactgatgt ttgtctcac gcgaaataa tgagacgag ttcaaatcg gaggcctcc aaaacgtgt ttcctcaag tagttgaga cgattttac acgaagcaa gagacgaat ttattgaaa caatccatg gagtgagtg ttaatttaa aaacgaaac ctttggatc acactgatg gacacataa agcttgagt cgttcaaat ttttgggaa aatcgaaac gctacgatg tttttttga ccaacagaa gtaaaatgt taaagaagt ggaaactaa atcatcccc taatccgcg caattctca acgaatgtg tcacgtttc taccggaca agattttcg attttgggg ctaaaaaca acgaaccga ttgatatcg cagtgttga acgatattt tccacaatt acaccaaag aagctaaaa ccttgttca aagatttgt acacaagac tcttcaatt tggtacaga aagacaaaa gcaaaggtc gtgatcatt tgccagctt gcactgggc ttttttcgg tccacttaa gtcaaacaa tcatcgaaa ccatgaaat atatcacta gagtaacag gggacgaag acttgattc cgtacctca aactcactt taatttttg gacaagaag gactttcct tgaaaccaa caatggaag aagtgattg ggctataca gacgatttt tcaacgaac ttgttgcaa ctggtgaaa aatttcccg tgcttgaat cggcaaaga atgaagatt tggaagaga aaattatgt atcttaatg taactgaag attttattg attcatgtg ttgaccaaa tggccagca atatagcca agatgtatg aacccattt ttgcatatc ttgattacg ttgagtctg aatttggtg atgtgtatg ctgattttg gcgacgaag aagaaatga ctttcacca aaggtttca accaaattg cttactaca aaagctaca tcaaaaaag caatgacta acctaacca atgaatatt ttggcattg gtcaactca tcttgtcgg aatacgttg ataatttga aagcttccc ttcaacaaa tcattgatg atacccacg ccaaaatca gtctggaac atagacgag aagacgaag caatttgtc aatgttttc aagacattc aagaaaagg cacttgaaa ttccagcca ataccgaag aactcatcg cccaaggta aacacatga tttgggtca agacagttc aactcaaag aactaagag atcgcatcc aaatgatgc tacacaatt tggtgaaac tgatcgact ttggtgaaa tcacacgcg accacatgg acttgaact ccgacactg tcaactggt tgaccttga tcgaaccag tccttgaac acaactcca ccatgtacg aacaattca aattcgaag cggaggaaa agctccaga aaacgaaag aagaaattg ttttcgcgc tcactgaac tggaaccca aacttgtga ttctgaacg aaatggacg atgtaatca atgtgagac aatacgtac gtcaaatgg ccccatttc tcgaagtga tgcgagacg tccgaacca aaataatct ggtccaaca aggaagaga caagtttgg ggttcgggg ttacgactt tcaacaaat acgaagaag tggcaaact ttctgttcc cgttttacc acttgttga agctttgcc taaacttgg aacgcaaaa ttggagttt ggttcgacg gccagtttg agtttctcg atgccgagg agaccgaag cccaaattg aggagttcc agaaggagt tgctcaaaa tacaaaaaa cctatcgga ttaagttgc gccaagcac aagctgaga atatttcgc tgcgttttg atggaattg ttgatgatc ctgagctcg tgaactggc ccgctccgc taaaactca ccgcgaaag caatccaga atttgaagg actttcgac ctgcgttaa ccatgatga agattatgt gtaacgatg ctttgttga agcgacact ggaaggaga tgtccgaag ttgcaggtt tcgacatga ctccaaatg ctggaactt ctctgcgaa agcttatga tgatggggc ttgaagctg atattgaca agtacgaaa ttatcagtt ctagtgcca ccaaagaac gcgaactgt tgaaaaatc tgaataaaa tgcaggcgg agtgggttg atatttgtt tcaagactg gaatttaca aggatacgg ggatccata tcttgactc agttggatg atattcaag ttgtgctag acgaccaca ttttgaaga ctttgacga tgcgaggat cagtctttg tgagacctt acgaaaccg aggttaaga gcttttacg agaagttga ctcgaatca acagtacga ttgaagagt gggggaagg tgcaatcac aatggttgt acttgctgc cgattttct cgtccaaag acattgtga gccaaatgc ctgaagaag gcctacttt tcaaagaag tgaacgata cgtacaagc gttacatgg acatggtct tgagggacc ctcgagtca cggaaaccg ctggtgcga tgggtgttt tggaagcaa tggttcatt gtatcgagc ttttggaga aaattaacg atggcgtag tttcgtatt tggaacgca aacgattat ttttcccac gtttcttct tcctatcca acgacgaga tgttggaga ttttatccg aaacgaagg acccactcc gggtgcaac cccacttga agaagtgtt ttgaagcga tcaatcggt taaatttcg acgaacaat tgcaaatcc atgccatgt acagtcaag aaggggaac aaatcgact ttgtcgaaa tgatcaaaa cgaaggaat ttggtggta gcgtcgaaa agtggttga ttttggtcg aagaccaga tggttaagt cagttcgtg agcaaataa tcaaaagtt ggaagaatt attttgtaa caccgcgaa ctaaatggg tccagaaat ggccaggtc aggtggtct tagccgtgt cacaaatgc actggactg cgcatgttc ataaagcgt tgaatcggg tcgaagaca tgtcgattg agtcgtttt ctgagcaac tcaagaaac aactccagg aaatagttg ggttgattc gtgatccaa ctctcacaa acttatcgc ggatcacaa tcaaagcct tgattgtga ttgatgttc atgcgaaag atgttgttc acgaacttc acgaaaaac gtgtcaagg acgataagg agtttaagt ggttgtcac aaatgaggt actaccttg aagacgacg agtcttacg tccgattga ttaatgcca cagttcggt atgcgtatg aatatttgg gaaatactg atcgtcttg tgatcactc ctttgactg atcggtgtt atcgaacgt taatcggag catatcatt tgcacttaa atggagctc ctgaagggc cggctggga ctgggaaaa ctgaaacta ccaaggatt tggccaagg caatagctg tacagtgtg tggtgttta attgttcag atgggttgg actataaag ctatgggga agtttttca aaggtttgg catcgtgtg gtgcttggg catgcttcg acgaattca accgaattg atattgagg ttttatcag tggttgccc aacaaatcc tctccataa tcatggcag ttcgtgcta atgcatcga aatttattt tcgaaggaa ccgaaatat ccctaaatc cagcttgtt acgtttgca tcaccatga atcctggct atgccggcc gaaccgaac taccagaca atttaaaag ttctgtttc gaacagttg caatgatgg taccagatt atgcaatga ttggtgaaa tttccttgt attcgtatg gttttatcg acgctcgga acctttcag tcaagatcg tcacgactt atcggctat gttcggaac aactatcgt cccaaaacc actacgatt atggtatga gagccgtca aatccgtgc tttcggcag ctgggaata acaaacgaa atttcccag acgaacatg aggatattt tgctcttga gggcgatac ttgatgtta atttaccca aatttctta atcaagacc ttccacttt tcgaaggta ttatctctg atttgttcc caggggttg tactccccg aagctgatt acaaaatgt taacaaacg ccatgaata tcagttgcc agaaacgca atcttcaac cgaaaagtt ccttcattt taaagatca ttcaaacgt acgaaatga tgatcgtta ggtggggtt ttatgattg ttggggagc ctttcgctg ggaaaactt caactttga aaattttgg ccgatactt taacattga tcaatagtc aagggttgg gcgaatcaa acgttcaat atacgattt tgaatccga aagcgatta caatgggac aactctatg gtcagtttg atccgattt cgtacgaat ggtcggatg gtgttgttg cgacttgtt ttcggaatt atgcccatg attcgagtc ctgacagga aatggatta tttttgatg gacctgttg atgccgtct ggattgaaa atatgaaca gtgtgttgg atgataata agaaacttt gtttgatga gtggggaag ttatgacct tgtcgagta ccatgtcgt tgatttttg aagtcatgg atttggagc aagcttcac cagcgactg tatctcgct gtggaatga tttacatgg aagctgtaa cactcggct gggaaccct tcatggaat catggattg taaattgta accctgact ggtgccacg atgaattta aaactctaa tcatcgata tcttcaaat ggattgtac ccccatgtc tttatttca ttcgaaaac aatgtgtgc aatactgca acattggaa acatcaact tggtgaaaa acatgatgc aactatttg aaatgactc taaacaagg ctttatccg agacggaag acacaaaaa atttactaa tctggatgc aggcagcat tgattcagg ccggtttat ggggacttg gttgtgtcc ttgattcag actcaaaga tcaaatttg acacgtttt acaagcagt tgtggcgga atcaaattg aggaatgtc catatccag cttcgatgg aaaaacttg agttgaata taccaatcg aagggttac tttacgact attcctatt attttcgaa tgaaaggta cttggaagt actggccgg aagtggtac gtaacgaac gagtcgaag agtgcgcaa atattttac aagcaatgg ttccaacag ttgataccg cacgttata tggctttgg tcgaccttc acatcaaaa ataaccttc cagttttat tggttgggc caacaggga ctggcaaaa gtttctata tccaagatc tgctaatga atcaattgg acaaggaac tgtacctac cttcgttta tcacattca ccgtgaaaa tcacagcga atcaaaccc aaaatttga tcatttcca agctaaaca aattgaggc gttcacatt atggagccc ctaaaggca aaacctgtg ttctcttca ttgacgata tcaacatgc cttataagg aaacatacg gtgcacaac cgcccatcg agttactcc gccaataca ttgatcaca agaactggt acgacctta aaaccaccg aaccgattt atttacaca acgttattt tcctcgctg ccatgggtc tggttggtg gtagccgcc aagaaatct atcctcgtt ttttgcgcc atttcagca tattctcga ttaatgagt tttcggaag aaacgatgg ccaaaatct ataccaaca tcctacatt tggggtgga aaaataacg gttttccga gcgatgtaa ttagcttag ctgtacaaa cagttggtg ctagtttag acatgtaca aatcagcga tggataatc taagaccta caccgtcga agagtcact acgtgttca atctgcgtg atttctcac gtttgattc aaggttgcg ccatgctac gtaaagagt gtgtggaaa gtaaggtta tttttgcga aaatttggg ttcacgaag tgttaaggg ttttctacg accgtttgg tagaagaaa ccgacaaga attggttgt ttgcgaagt taaaactag tagtgcgtg accatttca aggaacatt ttgatacaa tctttgata atctaccga aagactctg acggggttt tgacactca attccttga agaaactga tgtttggga cttactttg accaggatt caaacgacg atgaacgaa ggtatgagg aaacgatca atatcaagc aatttgccg atgtgtgcc aactgtgct tggatgagt acaattcaa ctcacaaga caaaaatgg atgttgtgt tattcgact atgccctcg aacacttgt ccaaaattt gtcgtgtct tgtcaatgg cttgcggta gttgcttat tggttggta tcagtggtt ccggtcgtc agtccttaa cccgactag caagtgaaa tttatagtc aacacttgt accaacctg aaatcacta ataactacg gtttgaacg aatggcgtg acgatatta agaaaatct taaaggaag gaggtggac gcggtcgcc attgtgtct tcctgattt ccgaaggtc aaatcaagg aagaatcgt tcttgcaag acattgatt gtttgttaa attcgggcg aagtcccga acatttatc aaatcgatg aacgacaag aaattttgg atatggtgc gtctggcgg cgcaaggag ggaaccgaa acttggata tttccgccc ttcaaatct tttatttct ttacgaaac gttgccgcg agaagttac acattattt tgtgcttca gtccggttg gttcaacct tccgcaatc gtttgaggc tttacccgt ccttgatta attgttgta cgattgatt ggtttgacg attggcccg aaaatgcct tagaagaag ttgcgttta aatggatgt cggatgtta acatccccc aggaggtga agaaccaag ctgtgatcg cgtgcaagt atttccacg tcgaggcaa ggcttgttt ccgatgaat tttataaag tatcaggtc gcaaaactt acatcacct cagcttcgt atctcgagt tgatcaaat cgttcacaa tcctgacta atgcgaaac agttggaga ttttaacaa ctcagaaac gttacattg gtggtttga agaaactac tcttcgcgg cggaacaaa tcgccgaaa tgcaaaaaa acctggcga acttgcaac cacaacttg aagaaatga accaaaagg cgattgata tgatgaaac aaatcgaaa gtgagacta ttgaggtgg agaaagctt ccgagttgg tgaaaaagg acgagaaag ttgcaaata ttcaagctg caatggctt cggccttga aacgtgagt gtgaagctg atttggccg aagctatac caattcttg aggacgcga tacgtgcct tggacacac tcaaaccag ccgatatca ctttggtca aatcaatga aaaacccac cagctgcaa tcaaacttg tgatggctg ctgtttgca taatgaaag atgttaagc cggcacgta ttcgtgacc cttcgactg gtcgaatga acttggatt tttggggtc cgagtgtga aagttttgg gcgatatga acttcctcc aaagtctca aagactacg acaaggacc acatcaagc ccgaaatta tggttaaaa tacgtagag agtttctcc cccacaagg attttaaac cagcagttg tggcgaagg catcgagtg ctgccgaag gtctctgta agtggatta tagccattg ataagtacg acgaggtca ataaaattg ttgcaccta aaaaagaaa agctggata aggcggaac gcgagttcg ctgccacta tggaaatac tacgggcga agcgcgaac aagtcaaac agttggaag agaaattgg cgattttga acgaacagt tgggcgaag ccgtccaga aacaaggcg aacttcaag ccgatgtgg acttgtgca aaaataaat taatacggg cccaaaaat tgatcggtg gacttggtg gtgagaaaa ccaggtgga cagctgcag ccaattcac ttcaagaac aacacgatt cattggctg gcgatattt tgatttcca gtggcatta tggcgtatt tatcaccct tgactgcgt tctaccgcc aaaaaactg tagccgact ggcacaaaa tcgtcaaag agttgaaga ttccatgtt ccgacacgt ttgatttcc tgtttgttt tgggttcgg atgttaagg tacaaaact ggtacatca gtgggctac cacgcgata gtttctcga ctgaaaatg gcataatag cggacaatt cgcggaaat ggtcccttt tcattgatc cccaatacc aggccagta actggatca aaaaaatgg agaagaaga attcgctct acgttgtta aattctccc actccgact atatgaaaa aaatcgaaa attgcgtcc agaacggat atcccgtcc tgatcgaaa gcattgggg aggttttgg aagcaccaa ttgatcctc tcctgtaca aaaaagtgt tcaaacaag ctgggttcg aggtgatca gtatggggg aaaacgtaa tcccttaca atcgaaact ttagattgt atcttgcat cacccttga ggaacccac actatttac ccgaagttt tcaatcgtg tgacaatta tcaactttg ccctaactc tcgaagggc tccaagacc agttactgg ggattgtgg tcgcagtgg aaaaacccg aattgcagc gactcaaag aagaactga ttgtgcaaa aagcggaaa acaaggctg cgctagaca acgtcgagt tgaacattt tgcgaacgc tttcggaat gtaaagggg atatccttg aggatgaat ccgcgatcc aaattctag acgaatcaa aattgttgt ccatcacaa ttcgcgaga aacagagca agtctttgg aaattgaaa agacaatcg aggagtttc gcgttttgt accaagggg tagccgaac attccgccg ttctctact attgcattt gtgacttgg ggaacgttg acccaatgt atcaatact ccctcgact ggtttataa acttataca ttggttcaa tccaacgag ccgagaaat gtaaaagtg tggagaaac gttgcacca atttaataa cagccttca cgtttgatc tctactcca atatcacac gttccctct tcgagaagg ataagttat tgttttcct tcttgcttt gctccaaga tactgattt ttcgcaaag cgctcgatg agaaggagt tcatgtttc ttttgacgg gtggtgtta atgttgaaa atccggtcc caaaccccg tcaattggt taccaaacc aggcgtggg acgagatat gtcgagtgg atgaattac cggcgtttc acaattttc gcgattctt tcgttcaag cgagtcgcg actggaaga ggatttacg atagctacg aaccggaga aggaaagat tgccagggc cttggcacg acaaactta acacgtttc gaaagttga ttgtgattc gcattctgc ggtccgata agttgatag tgggtgcca ctgattata ttgcaaagg agatggggg tgaagtata tcacgccgc ctcagttta acatttcca tttcgtaca atgagtctt acaatttgt gtccgttga tttttatat tgtcacccg ggactgatc caatgtccg cgctagtga aattcgcag aagagaaga aatatacgg agaagtttc aatcaattt ctttggggc aaggtcagg ggccacgtg ccgcagctt tgatccagc aaggtcaag acgaaggat tatgggtgt gtctgcaaa attgtcact tggccacgt cttggatgc caagtcttg aaaaaatct tcgaagata tggattata ataacacta atgataact tcaggttgt ggctaacga gttacccgt ctgataagt tcccagtgt caatactcc agaaaggag tgaaaatca ccaacgaac caccgactg gtttgaaag acaacttgt tgaaatctt acgttaacg atccggtga agaaccaag atttttaca acggttgtc ccgggaatt acgagatgt ttgtcaaac tgttgtatg gcattgcgt tttttcacg ccgttgtgc aagaacgcc gaacttttg gcccacttg gttggaata ttccgtacg gttttaacg attcggatt ttgatatca gtgtccaac aattgcaaa tgttcatca atgagagtg aaaatccgt atgaagcat tatcgtacc tgattggag agtgtaatt acggaggtc gtgtcacag atgattggg atcgtcgat tgattacta caattctag aggattttc ttaatcctc gagtcgcaa cttcgaaca attatcttt ttagtgacg cggcggatt gttatggcc ttccgatta aaatcgact atcaagatt ttgtcaccc acatccaaa atttaccac atgtgcacc ctccgcaag tgtttggac ttaacacaa atgctggaa taacgcgcg atatgcaaa actcgcaac gcttgttaa attccgtcc ttaaagctt acggtgaaa ttcaaactg gtggtgttg gtgaaaccg acaagtaca tcatgatgt tgtgttccg atatcttat ccaaattac caaaaccgt tcgatcagg aagaagcaa ttcgtcgtt accccgtcc agtattcgg aatcaatga acactgtgt tggtgcaag aaatggatc gtttcaacc gactgcttt ccaccatcc gcaactctc taatcaaca tgcagaaag cgatacaag gactggttt caatgaccc cttcccttg aatccttcg cctcttctc tgctaatcg ctcgaatcc cttcaaact gggcgagca gttcgtatc caagcctca aaaacttgc cccattacg tggccgatt ttttgtcaa ggattgagt ttctcgaaa cttggttca agcgtggta aaccggatg actattgga tttctgggt ttttcttca ctcaggcgt ttctaaccg gtgttaagc agaattacg caaggaagt atacaattc cgattgata agcttacgt ttgattttg agattttgc aaaaagatc gtggagata gagcaccaa aagatgggg cgtatattt atgggttgt ttactgatg gggcgagat gggatcgtg ccagaggac aaattgatg agttgctgc caaaggtgt tgcatgata atatgccct tgatttgga ttaaaccga tcaaagata aggactata aagagagag gaaggtata ggtgtccgg tttataaaa cttcagaga ggagagggg tgttgtcga caacggggc actcaacga attatgtgc tgccgatct tgatggaaa caagtgtga aaccggcgc attggatta agaggagtg tggcattgc tctgtcagt tggattaaa cacagggga aaattgact tgtagcatc ataaataaa cgacaaaaa atact
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