Status
Please wait ...
Gene information
General information
TC010958 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 2026836..2028651
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC010958
      iBeetle screen iB_04949

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC010958 RA
        MKLLILGHL FLSLHFGRG DNEPNAPKP 
        EEMDQDLRV VNETIAKYG PPKDYKIFN 
        LPGPEILVD DNLLLHPVS YNSRYEQTE 
        LQHIGLESF PVVKGQFGE HFIRKSGSF 
        YIRTIGYVL DRNGYQEFL IDISKTETT 
        VKEFVPPLV ESDIYVDRR PPGKIQISS 
        TVLATLVGG NSLG
      CDS sequence
      • >TC010958 RA
        atgaaattg ctaatcctt ggtcactta ttcctgtcc 
        ctacatttt ggtagaggt gacaatgaa ccaaatgca 
        cctaaaccg gaagaaatg gatcaagac ttgcgcgta 
        gtgaatgaa acaatcgca aaatacggt cctccaaaa 
        gattacaaa atcttcaat ttaccggga cccgaaata 
        ttggtagac gataacttg ctgttgcac ccagtcagt 
        tacaatagc cgttacgaa caaaccgaa ttgcaacac 
        atcggctta gaaagcttt ccagtggtt aaaggccag 
        tttggtgaa cacttcata cgcaaaagt gggagtttt 
        tacattcgt actattggc tacgtactg gaccgaaat 
        ggctaccag gaattttta atagatatt tcgaaaact 
        gaaactact gtgaaggaa tttgttcca ccacttgtt 
        gaaagtgac atttatgtt gatcgtcgt cctccagga 
        aaaatacaa atttcgtcg acagttttg gcaactttg 
        gtgggagga aattcactt ggatag
      mRNA sequence
      • >TC010958 RA
        ctgttttat tatctataa gcgtgacct aaatcaatt 
        ttttgtgta tggacagga caccaatat tttcagcat 
        ttatctatt taaaatcga aaataagga tatttagtt 
        gtatgttca gtaaaataa taacctaat tgacgtaga 
        ttatctgtc aagtgacgt aaactgatg cacttttgt 
        gcaacaatc tgtttattc cacccacgg aagcaacag 
        caaaacaac atgattttg gtactttca cttttacag 
        aatccctta cttgacata atataatta taaattatt 
        tccagtttt acttcatat ttggatttt gtctcattc 
        cggccttta tttgtgacg aagaaccaa caattgaag 
        atttgcacg tactaaacg taacaatag caaaatatg 
        gtcctctca aaaactacc aacagctgg gtttaccag 
        gccccgaaa taaaaactg acaaaaata aattttcac 
        tggcgaata tattcgaat ctggtaaag cggagaatt 
        atactttga gggttcgta caataactc cagacttca 
        gttacaatg cacgttatg aagaaacca aattgcaaa 
        aagttaacg acagtttcg agcattttc cggtaatta 
        aaggccagt ttggagaac attttctca gagaagatg 
        gaggttttt acattcgta ctattggtt acgttctgg 
        acgataaag ggtttcatg aatttttct tggatctta 
        ccacagctg taacacaga catttcttc cacttttgg 
        gtacaggca ctgttccta tttcttcaa cagtactta 
        ttacactga acggaggaa attcacttg gctagagcc 
        taacctgtt ttggattga tcttataat aaataaaca 
        caacaccca gttatttgt ttatttttg ctcatacat 
        taattagtg tgcagacca gtcgcttga caaggtttt 
        tgcacttgg tacaaggta aagttatga agctcttaa 
        ctggtttga tttgtattg tatttaaac agtggataa 
        gttaacatc atgaaattg ctaatcctt ggtcactta 
        ttcctgtcc ctacatttt ggtagaggt gacaatgaa 
        ccaaatgca cctaaaccg gaagaaatg gatcaagac 
        ttgcgcgta gtgaatgaa acaatcgca aaatacggt 
        cctccaaaa gattacaaa atcttcaat ttaccggga 
        cccgaaata ttggtagac gataacttg ctgttgcac 
        ccagtcagt tacaatagc cgttacgaa caaaccgaa 
        ttgcaacac atcggctta gaaagcttt ccagtggtt 
        aaaggccag tttggtgaa cacttcata cgcaaaagt 
        gggagtttt tacattcgt actattggc tacgtactg 
        gaccgaaat ggctaccag gaattttta atagatatt 
        tcgaaaact gaaactact gtgaaggaa tttgttcca 
        ccacttgtt gaaagtgac atttatgtt gatcgtcgt 
        cctccagga aaaatacaa atttcgtcg acagttttg 
        gcaactttg gtgggagga aattcactt ggatagaaa 
        ctgagctct ttcgaattg tactcatcc aattttatg 
        caaaatcaa agaaaataa atccaaatc t
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY