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Gene information
General information
TC033000
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010956
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 2012346..2016805
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC033000
      iBeetle screen iB_04948

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC033000 RA
        MKSFFVLIF GVSLAHFGQ CAEEPTSSP PLPDPSEDM 
        RVLDDTIAK FGPPQDYKL MGFPGPEIA LDEEKYVHK 
        NNLELKGSG NYTLKTRTV TPYWSYNRH YEDVKMPQS 
        FDQPPDIKG QFSKHFGRK SGGFYIRTI GYKIDENGY 
        RQFLLDLTK VETTLKKVF RPSLYYCSG NTNNLVFSF 
        LGPTGTANA STTVVTPLT GNVLKQNNN NMKLFFVII 
        VTSSLHLSR CAKEPGTQS VTKNPADAT EDLRVLNEA 
        IAMYGPPQD YKKLGFPGP KIALDSEKY TYFNQLQYE 
        NGSDNYILR IRTITPNFS YLGHYEEVK MPDNFTQFP 
        EIKGQVGEH FARKNGGFY VRTIGYKVD ENGYRQFLL 
        DLTIANTIT KEVTESPTA PLTYCGVDY LYTVGFGGT 
        TTTSAGSSA SSYGGFGGG TQAKTTISS TVLLTLTGG 
        NTLG
      CDS sequence
      • >TC033000 RA
        atgaaatcg tttttcgta ttgattttt ggagtttct 
        cttgcacat ttcggtcaa tgtgcggaa gaaccaact 
        tccagtcct ccactacca gacccttcc gaagacatg 
        cgtgtccta gacgacaca atcgccaaa ttcggccct 
        ccccaagac tacaaacta atgggtttt ccaggcccc 
        gaaatagct ctagatgaa gaaaagtat gtacacaag 
        aataacctc gagctcaaa ggttcagga aattatact 
        ttgaagacc cgtacggta accccgtat tggagttac 
        aataggcat tacgaagac gtgaagatg cctcaatct 
        tttgatcaa ccaccagac attaaaggc caattttct 
        aaacatttc gggcgaaaa agtggagga ttttacatt 
        cgcaccatt ggttacaaa atcgatgaa aacggttac 
        cgacaattt ttattagat cttactaaa gtcgaaacg 
        accttaaag aaagttttt agaccttca ctgtactat 
        tgcagtggt aatactaat aaccttgtc tttagtttc 
        cttggtcca acggggacc gcaaatgcg tcaacaaca 
        gtagttaca ccactgaca ggcaatgtg ctgaagcag 
        aacaacaac aacatgaaa ctgtttttt gtaatcatt 
        gtcacttct tccctacat ctctctcga tgtgccaaa 
        gagccagga acccaatct gtgacaaaa aacccagcc 
        gatgccact gaagattta cgtgtcctg aacgaagca 
        atagccatg tacggccca ccacaggac tacaaaaaa 
        ctgggtttt ccaggccct aaaatagcc cttgacagt 
        gaaaaatac acgtacttc aatcaatta caatatgaa 
        aatggatct gacaattat attttaagg atacgcaca 
        attaccccg aactttagt taccttgga cattatgaa 
        gaggtgaaa atgcccgac aacttcact cagtttccc 
        gaaattaag ggccaagtt ggggaacac tttgcacgg 
        aagaatgga ggtttttac gttcgaacc attggttat 
        aaagtcgac gagaatggt taccggcaa tttttgctc 
        gatcttaca attgctaat acaattacg aaggaagtt 
        acagagtct ccaacagct ccgctaaca tattgtggt 
        gttgattat ctttatacg gttggtttt ggtggtact 
        actactact agtgcaggg agctctgct tctagctat 
        gggggattt ggaggaggt actcaagct aaaaccacc 
        atttcttct acagtgctt cttacgctt accggaggt 
        aacacactt ggctaa
      mRNA sequence
      • >TC033000 RA
        ttctatttt ttcaaatac ttttgcttc ttacattag 
        aagcagctc aagacaaca tgaaatcgt ttttcgtat 
        tgatttttg gagtttctc ttgcacatt tcggtcaat 
        gtgcggaag aaccaactt ccagtcctc cactaccag 
        acccttccg aagacatgc gtgtcctag acgacacaa 
        tcgccaaat tcggccctc cccaagact acaaactaa 
        tgggttttc caggccccg aaatagctc tagatgaag 
        aaaagtatg tacacaaga ataacctcg agctcaaag 
        gttcaggaa attatactt tgaagaccc gtacggtaa 
        ccccgtatt ggagttaca ataggcatt acgaagacg 
        tgaagatgc ctcaatctt ttgatcaac caccagaca 
        ttaaaggcc aattttcta aacatttcg ggcgaaaaa 
        gtggaggat tttacattc gcaccattg gttacaaaa 
        tcgatgaaa acggttacc gacaatttt tattagatc 
        ttactaaag tcgaaacga ccttaaaga aagttttta 
        gaccttcac tgtactatt gcagtggta atactaata 
        accttgtct ttagtttcc ttggtccaa cggggaccg 
        caaatgcgt caacaacag tagttacac cactgacag 
        gcaatgtgc tgaagcaga acaacaaca acatgaaac 
        tgttttttg taatcattg tcacttctt ccctacatc 
        tctctcgat gtgccaaag agccaggaa cccaatctg 
        tgacaaaaa acccagccg atgccactg aagatttac 
        gtgtcctga acgaagcaa tagccatgt acggcccac 
        cacaggact acaaaaaac tgggttttc caggcccta 
        aaatagccc ttgacagtg aaaaataca cgtacttca 
        atcaattac aatatgaaa atggatctg acaattata 
        ttttaagga tacgcacaa ttaccccga actttagtt 
        accttggac attatgaag aggtgaaaa tgcccgaca 
        acttcactc agtttcccg aaattaagg gccaagttg 
        gggaacact ttgcacgga agaatggag gtttttacg 
        ttcgaacca ttggttata aagtcgacg agaatggtt 
        accggcaat ttttgctcg atcttacaa ttgctaata 
        caattacga aggaagtta cagagtctc caacagctc 
        cgctaacat attgtggtg ttgattatc tttatacgg 
        ttggttttg gtggtacta ctactacta gtgcaggga 
        gctctgctt ctagctatg ggggatttg gaggaggta 
        ctcaagcta aaaccacca tttcttcta cagtgcttc 
        ttacgctta ccggaggta acacacttg gctaacttt 
        ttgtaccat tgtcccatt tttactttt gaaataaaa 
        ttcttttct ttgt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY