Status
Please wait ...
Gene information
General information
TC010955 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 2001836..2003991
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010955
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04947

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010955 RA
      MLPIVLFIL ACTICYFFV KINHAYWKK RGVVGPKPS 
      FLVGNLGKS FILKSSPGE IYTEIYRKY KDASVVGLF 
      RSETPVLLV RDPELLKEI TTKSFQHFR NNDIDVDKK 
      HDPLFGRNP FVLKGDEWK TVRAQLTPG FTSGKMKWL 
      YSYLETNSK KMVEYILRV PEATNGQGY EAKELCIRY 
      TLNNVGDAV FGIEGKCFE EKNSEFRKL SKEFLSPGS 
      YGIIIMFLA TLFPPITKI FPMRFVTKS VEKKLTNLV 
      SDALKHRQV NNIVRNDFL HIISQLKKT NQDFTDVDV 
      TAHAAGFFA DGSETSSIV MSFVLFQLS INPDVQSKL 
      RAEVTEAFN NNNNNMPYE VVQDLPYLE AVIQETLRI 
      QPPIHSLQK LCTKSFTWT LKETNKPLV IEEGTPIII 
      PTHALQSDP QHFEDPESF QPERFLGEN RENIKKCTH 
      MPFGEGPRA CLGQRFGLL QIKVGLAYI VKNFELSLN 
      KKTKLPLKY DPINPLVTP TGGLWIDFK KIEKKKCNQ 
      IKSFHVMYL VIFPNIL
    CDS sequence
    • >TC010955 RA
      atgctacct atagttttg tttatcctc gcttgcaca 
      atttgctac tttttcgta aaaataaac catgcatat 
      tggaaaaag cgtggcgtc gttggcccc aaaccatca 
      ttccttgtg ggaaatctc ggaaaatcg ttcatctta 
      aaatcatca ccaggcgaa atatacacc gaaatatac 
      aggaaatac aaagacgct tccgtcgtg ggcttattc 
      cgatccgaa actcccgtc cttttagtt cgtgatcct 
      gaattactc aaagaaatt acaacaaaa tccttccaa 
      catttccgg aacaacgat atcgatgtg gacaagaaa 
      catgaccca ctttttggc cgaaatccc tttgttttg 
      aaaggagat gagtggaaa actgtgagg gcccaacta 
      acccccgga tttacaagc ggaaaaatg aaatggttg 
      tattcctac ttggaaacc aacagtaaa aaaatggtc 
      gagtacatc ctgagagta ccggaagcg accaatgga 
      caaggctat gaagccaaa gaactgtgc atccgttat 
      accttgaac aacgtgggt gatgcagtg tttggcatc 
      gaggggaag tgttttgag gagaaaaac agcgaattc 
      cggaaactt tcgaaagag tttttgtcg cctggaagt 
      tacgggatt attatcatg tttttggca actcttttt 
      ccgcctatt actaaaatc tttcccatg agatttgtt 
      acaaagtca gtcgaaaag aaactcacg aatttggta 
      tcagacgcc ttgaaacat cgacaagtc aataatatc 
      gttcgcaac gattttctg cacattata agtcaattg 
      aaaaaaact aaccaggat tttactgat gttgatgtc 
      actgctcac gcggctgga ttttttgct gatggttct 
      gagaccagt tcgattgtg atgagtttt gttttgttc 
      caattgagc ataaatccg gacgtgcaa tcaaaattg 
      agagcagaa gtaactgaa gcgttcaat aataataac 
      aacaacatg ccctacgaa gtagtgcaa gatttgccg 
      tatttagag gctgttata caagaaacg ctacgcatc 
      cagccgccg attcactcg ttgcagaaa ttatgtacc 
      aaatcattc acttggacg ctaaaagaa acaaataaa 
      cccctagtt attgaggaa gggactccg attataata 
      cccacacat gcacttcaa agtgacccg caacatttt 
      gaagatccg gagagcttt cagcctgaa aggtttctg 
      ggagagaat cgggaaaat atcaaaaag tgtacccac 
      atgccattt ggggaagga ccaagagcc tgtttagga 
      caacggttt ggtttgttg caaattaaa gtcggattg 
      gcttacatc gtcaaaaat tttgaatta tcgctaaat 
      aaaaaaaca aaattgccg cttaagtat gaccccatc 
      aatccattg gttacacca actggcgga ctctggatt 
      gactttaaa aagattgag aaaaaaaaa tgcaatcaa 
      attaaaagc tttcatgta atgtacctt gtaatattt 
      ccaaatata ttataa
    mRNA sequence
    • >TC010955 RA
      ttagcattt atgtcatat ggatcaaat gaattgcta 
      ttttttact tgagtagga ggtattatg tgctctaaa 
      aaatatccg aatcgaacg cctgttgaa aatatgatt 
      cgtcatgtc acaatttat ccgatcatg tttacaatt 
      atattaaat tgaccttgc tctctatca aaagttgtt 
      agataagta catgtagtc agaattgaa gccgcaatg 
      ctacctata gttttgttt atcctcgct tgcacaatt 
      tgctacttt ttcgtaaaa ataaaccat gcatattgg 
      aaaaagcgt ggcgtcgtt ggccccaaa ccatcattc 
      cttgtggga aatctcgga aaatcgttc atcttaaaa 
      tcatcacca ggcgaaata tacaccgaa atatacagg 
      aaatacaaa gacgcttcc gtcgtgggc ttattccga 
      tccgaaact cccgtcctt ttagttcgt gatcctgaa 
      ttactcaaa gaaattaca acaaaatcc ttccaacat 
      ttccggaac aacgatatc gatgtggac aagaaacat 
      gacccactt tttggccga aatcccttt gttttgaaa 
      ggagatgag tggaaaact gtgagggcc caactaacc 
      cccggattt acaagcgga aaaatgaaa tggttgtat 
      tcctacttg gaaaccaac agtaaaaaa atggtcgag 
      tacatcctg agagtaccg gaagcgacc aatggacaa 
      ggctatgaa gccaaagaa ctgtgcatc cgttatacc 
      ttgaacaac gtgggtgat gcagtgttt ggcatcgag 
      gggaagtgt tttgaggag aaaaacagc gaattccgg 
      aaactttcg aaagagttt ttgtcgcct ggaagttac 
      gggattatt atcatgttt ttggcaact ctttttccg 
      cctattact aaaatcttt cccatgaga tttgttaca 
      aagtcagtc gaaaagaaa ctcacgaat ttggtatca 
      gacgccttg aaacatcga caagtcaat aatatcgtt 
      cgcaacgat tttctgcac attataagt caattgaaa 
      aaaactaac caggatttt actgatgtt gatgtcact 
      gctcacgcg gctggattt tttgctgat ggttctgag 
      accagttcg attgtgatg agttttgtt ttgttccaa 
      ttgagcata aatccggac gtgcaatca aaattgaga 
      gcagaagta actgaagcg ttcaataat aataacaac 
      aacatgccc tacgaagta gtgcaagat ttgccgtat 
      ttagaggct gttatacaa gaaacgcta cgcatccag 
      ccgccgatt cactcgttg cagaaatta tgtaccaaa 
      tcattcact tggacgcta aaagaaaca aataaaccc 
      ctagttatt gaggaaggg actccgatt ataataccc 
      acacatgca cttcaaagt gacccgcaa cattttgaa 
      gatccggag agctttcag cctgaaagg tttctggga 
      gagaatcgg gaaaatatc aaaaagtgt acccacatg 
      ccatttggg gaaggacca agagcctgt ttaggacaa 
      cggtttggt ttgttgcaa attaaagtc ggattggct 
      tacatcgtc aaaaatttt gaattatcg ctaaataaa 
      aaaacaaaa ttgccgctt aagtatgac cccatcaat 
      ccattggtt acaccaact ggcggactc tggattgac 
      tttaaaaag attgagaaa aaaaaatgc aatcaaatt 
      aaaagcttt catgtaatg taccttgta atatttcca 
      aatatatta taatgatga aattgagtc ttattattg 
      tacagttat aatgcattg tcgtgagta aaaggcgtt 
      tacaaaaaa ttaaaatgt tttcagaaa aagatggtg 
      agggacaaa taaaaccgt gacccgaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY