| General information | |||
| TC010953 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 1919245..1927763 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC010953 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04946 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC010953 RA
MGEQCGKSL NNEFKEERE NKKQCSKFK EYLNNVSYE QFVRFMYQP KDPSSLGVI RFLFGLLML IDLPEERGG SDIDFRWGD PRDCHFPLF PILRPLPYP YMALLYGIM WFGACGIML GYKFQTSAL LFGVPYWYL LLLDKSFWN NHSYLFGIV TILLVGSSA NHYLSLDGL FDERKKNRH VPYWNYFIL KFQFFMLYF LAGLKKTDK EWLEGYSMT NLGNHWVFL PFTLFLTVE QIDYLVVHW FGFLLDLTI GFWMLIEIT RPIAMIFCA CFHLMNSRL FSIGMFPYV CLATMPLFC TETWPRRLQ SIFFKKVNY RVEPSPVCV YTRQQSEDY HDLQLPHEI NWKHKLVVG LLLTHCGLQ CFLPYSHFI TQGYNNWTK GLYGYSWDM MVHSWDTTI VVVKVVDNN SGGEFFLDP AAWTQNERW NKHADMCLQ YAECLKNNL LQDLQHEKS REGVENEVG PKHITSENI SIYVDVWCS LNGRFQQRM YDPNYDLLK ANWSPFRPV EWLKPVLAE YNDFRGQMN QISQEVYSW SNYSDVLFI ADFPGLYLE NYITKDLRN VTLTVLEGE VFFEIEDEE TSQSYGVKL GKNESVPVE VEVFHKVHT ISSTPSCYM YVYMNKTRE YLGELPQSS DSDRDVMYS PFPLIEDVQ FRIDAFLRM VGHISNSFL HVFYNQPLI RRSRLPFS
- >TC010953 RA
atgggggaa caatgtggt aaaagtttg aacaacgaa tttaaggaa gaaagagaa aataaaaaa caatgctcc aaatttaag gaatattta aataatgtt tcttatgaa cagtttgtt aggtttatg taccagccc aaggatcca tccagtctg ggagttatt cgcttttta tttggtctg ttaatgtta atagacctg cctgaagaa cgcggcggc tctgacatc gacttcaga tggggagat ccccgagac tgtcatttc cccctattc ccaattttg cgcccattg ccataccca tacatggct ctactttac ggaattatg tggtttggg gcttgtggc atcatgctg ggctacaaa tttcaaact agtgcgctg ttatttggc gttccttac tggtactta ttgttactg gataaatcg ttctggaac aaccatagt taccttttc ggcattgtt acgatactt ctggttggg tcatcagca aatcactac ttgtcgctg gatggactt ttcgatgag aggaaaaaa aatcgccac gttccgtac tggaactat tttatttta aaatttcag ttctttatg ttgtatttt ttggctggt ttaaaaaaa accgacaag gaatggctt gaggggtac tcgatgacc aacttgggg aaccactgg gtgtttctc ccgttcacg ttatttctc accgttgag caaatcgat tatttagta gtgcattgg tttggtttt ctcctcgat ctcacaatc ggtttttgg atgctgatt gaaatcacg cgaccaatc gcgatgatt ttttgtgcc tgtttccat ttaatgaac tcgcgtctt ttcagcatt gggatgttt ccgtacgtc tgtctggcc acaatgccg cttttttgc accgaaacg tggccccgg cgcttacaa agcattttt ttcaagaaa gtaaactac agagtggag ccaagtccc gtgtgcgtt tacacgaga caacaatca gaagactat cacgattta cagcttccg cacgaaatc aattggaaa cataaatta gttgtgggg cttttattg acccattgc gggctccag tgcttcctg ccctattcc cattttatc actcaaggg tacaacaac tggaccaag ggcctctat ggctactcc tgggacatg atggtgcat tcctgggac accacaatt gtggtggtt aaagtggtc gataataat tccgggggt gagtttttc ctagacccc gcagcgtgg acacagaat gaaagatgg aacaaacac gcggatatg tgtctacag tacgcagag tgtttgaaa aataatttg cttcaagac ttgcaacat gagaaatcg cgtgaaggt gtcgaaaac gaagtcggg cctaagcac attacttca gaaaacata tcaatttat gtagacgtt tggtgttct ctgaatggg aggtttcag cagcgaatg tatgacccg aactatgat ttactgaag gccaattgg tcgcctttc cggccggtg gagtggctg aaaccggtc ctggctgag tataatgac ttcaggggg cagatgaac caaatcagt caagaggtg tactcctgg tccaactac agtgatgtt ctgtttatt gcagacttt cctggcctg tatttagaa aactacata acgaaggat ttgagaaac gtgaccctc accgtgttg gaaggtgag gtgtttttc gaaatagaa gacgaggag acgagccaa tcttacgga gtaaaactc ggtaaaaac gagagcgtc cccgttgaa gttgaagtg tttcataaa gtgcacaca atcagttca acaccgtct tgctacatg tacgtttac atgaacaag acaagggaa tatttgggg gaattaccg cagtcaagc gacagcgat cgagacgtg atgtattca ccgtttcct ctaatcgag gacgtgcag tttcgaatc gacgccttt ttgcgaatg gttggacat atttccaac tcattcttg cacgtattt tacaatcag cctttaata aggaggagt agactgcca ttttcttaa
- >TC010953 RA
agtcaagtc tggaacgaa aaacttccc tggagcgca caacaatgg gggaacaat gtggtaaaa gtttgaaca acgaattta aggaagaaa gagaaaata aaaaacaat gctccaaat ttaaggaat atttaaata atgtttctt atgaacagt ttgttaggt ttatgtacc agcccaagg atccatcca gtctgggag ttattcgct ttttatttg gtctgttaa tgttaatag acctgcctg aagaacgcg gcggctctg acatcgact tcagatggg gagatcccc gagactgtc atttccccc tattcccaa ttttgcgcc cattgccat acccataca tggctctac tttacggaa ttatgtggt ttggggctt gtggcatca tgctgggct acaaatttc aaactagtg cgctgttat ttggcgttc cttactggt acttattgt tactggata aatcgttct ggaacaacc atagttacc ttttcggca ttgttacga tacttctgg ttgggtcat cagcaaatc actacttgt cgctggatg gacttttcg atgagagga aaaaaaatc gccacgttc cgtactgga actatttta ttttaaaat ttcagttct ttatgttgt attttttgg ctggtttaa aaaaaaccg acaaggaat ggcttgagg ggtactcga tgaccaact tggggaacc actgggtgt ttctcccgt tcacgttat ttctcaccg ttgagcaaa tcgattatt tagtagtgc attggtttg gttttctcc tcgatctca caatcggtt tttggatgc tgattgaaa tcacgcgac caatcgcga tgatttttt gtgcctgtt tccatttaa tgaactcgc gtcttttca gcattggga tgtttccgt acgtctgtc tggccacaa tgccgcttt tttgcaccg aaacgtggc cccggcgct tacaaagca tttttttca agaaagtaa actacagag tggagccaa gtcccgtgt gcgtttaca cgagacaac aatcagaag actatcacg atttacagc ttccgcacg aaatcaatt ggaaacata aattagttg tggggcttt tattgaccc attgcgggc tccagtgct tcctgccct attcccatt ttatcactc aagggtaca acaactgga ccaagggcc tctatggct actcctggg acatgatgg tgcattcct gggacacca caattgtgg tggttaaag tggtcgata ataattccg ggggtgagt ttttcctag accccgcag cgtggacac agaatgaaa gatggaaca aacacgcgg atatgtgtc tacagtacg cagagtgtt tgaaaaata atttgcttc aagacttgc aacatgaga aatcgcgtg aaggtgtcg aaaacgaag tcgggccta agcacatta cttcagaaa acatatcaa tttatgtag acgtttggt gttctctga atgggaggt ttcagcagc gaatgtatg acccgaact atgatttac tgaaggcca attggtcgc ctttccggc cggtggagt ggctgaaac cggtcctgg ctgagtata atgacttca gggggcaga tgaaccaaa tcagtcaag aggtgtact cctggtcca actacagtg atgttctgt ttattgcag actttcctg gcctgtatt tagaaaact acataacga aggatttga gaaacgtga ccctcaccg tgttggaag gtgaggtgt ttttcgaaa tagaagacg aggagacga gccaatctt acggagtaa aactcggta aaaacgaga gcgtccccg ttgaagttg aagtgtttc ataaagtgc acacaatca gttcaacac cgtcttgct acatgtacg tttacatga acaagacaa gggaatatt tgggggaat taccgcagt caagcgaca gcgatcgag acgtgatgt attcaccgt ttcctctaa tcgaggacg tgcagtttc gaatcgacg cctttttgc gaatggttg gacatattt ccaactcat tcttgcacg tattttaca atcagcctt taataagga ggagtagac tgccatttt cttaataaa aaatttaca acttattgt ggaagtgtt ttatttaaa ttgtccaaa tacaacatt cacaaaagt
