Status
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Gene information
General information
TC010953 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 1919245..1927763
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010953
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04946

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010953 RA
      MGEQCGKSL NNEFKEERE NKKQCSKFK EYLNNVSYE 
      QFVRFMYQP KDPSSLGVI RFLFGLLML IDLPEERGG 
      SDIDFRWGD PRDCHFPLF PILRPLPYP YMALLYGIM 
      WFGACGIML GYKFQTSAL LFGVPYWYL LLLDKSFWN 
      NHSYLFGIV TILLVGSSA NHYLSLDGL FDERKKNRH 
      VPYWNYFIL KFQFFMLYF LAGLKKTDK EWLEGYSMT 
      NLGNHWVFL PFTLFLTVE QIDYLVVHW FGFLLDLTI 
      GFWMLIEIT RPIAMIFCA CFHLMNSRL FSIGMFPYV 
      CLATMPLFC TETWPRRLQ SIFFKKVNY RVEPSPVCV 
      YTRQQSEDY HDLQLPHEI NWKHKLVVG LLLTHCGLQ 
      CFLPYSHFI TQGYNNWTK GLYGYSWDM MVHSWDTTI 
      VVVKVVDNN SGGEFFLDP AAWTQNERW NKHADMCLQ 
      YAECLKNNL LQDLQHEKS REGVENEVG PKHITSENI 
      SIYVDVWCS LNGRFQQRM YDPNYDLLK ANWSPFRPV 
      EWLKPVLAE YNDFRGQMN QISQEVYSW SNYSDVLFI 
      ADFPGLYLE NYITKDLRN VTLTVLEGE VFFEIEDEE 
      TSQSYGVKL GKNESVPVE VEVFHKVHT ISSTPSCYM 
      YVYMNKTRE YLGELPQSS DSDRDVMYS PFPLIEDVQ 
      FRIDAFLRM VGHISNSFL HVFYNQPLI RRSRLPFS
    CDS sequence
    • >TC010953 RA
      atgggggaa caatgtggt aaaagtttg aacaacgaa 
      tttaaggaa gaaagagaa aataaaaaa caatgctcc 
      aaatttaag gaatattta aataatgtt tcttatgaa 
      cagtttgtt aggtttatg taccagccc aaggatcca 
      tccagtctg ggagttatt cgcttttta tttggtctg 
      ttaatgtta atagacctg cctgaagaa cgcggcggc 
      tctgacatc gacttcaga tggggagat ccccgagac 
      tgtcatttc cccctattc ccaattttg cgcccattg 
      ccataccca tacatggct ctactttac ggaattatg 
      tggtttggg gcttgtggc atcatgctg ggctacaaa 
      tttcaaact agtgcgctg ttatttggc gttccttac 
      tggtactta ttgttactg gataaatcg ttctggaac 
      aaccatagt taccttttc ggcattgtt acgatactt 
      ctggttggg tcatcagca aatcactac ttgtcgctg 
      gatggactt ttcgatgag aggaaaaaa aatcgccac 
      gttccgtac tggaactat tttatttta aaatttcag 
      ttctttatg ttgtatttt ttggctggt ttaaaaaaa 
      accgacaag gaatggctt gaggggtac tcgatgacc 
      aacttgggg aaccactgg gtgtttctc ccgttcacg 
      ttatttctc accgttgag caaatcgat tatttagta 
      gtgcattgg tttggtttt ctcctcgat ctcacaatc 
      ggtttttgg atgctgatt gaaatcacg cgaccaatc 
      gcgatgatt ttttgtgcc tgtttccat ttaatgaac 
      tcgcgtctt ttcagcatt gggatgttt ccgtacgtc 
      tgtctggcc acaatgccg cttttttgc accgaaacg 
      tggccccgg cgcttacaa agcattttt ttcaagaaa 
      gtaaactac agagtggag ccaagtccc gtgtgcgtt 
      tacacgaga caacaatca gaagactat cacgattta 
      cagcttccg cacgaaatc aattggaaa cataaatta 
      gttgtgggg cttttattg acccattgc gggctccag 
      tgcttcctg ccctattcc cattttatc actcaaggg 
      tacaacaac tggaccaag ggcctctat ggctactcc 
      tgggacatg atggtgcat tcctgggac accacaatt 
      gtggtggtt aaagtggtc gataataat tccgggggt 
      gagtttttc ctagacccc gcagcgtgg acacagaat 
      gaaagatgg aacaaacac gcggatatg tgtctacag 
      tacgcagag tgtttgaaa aataatttg cttcaagac 
      ttgcaacat gagaaatcg cgtgaaggt gtcgaaaac 
      gaagtcggg cctaagcac attacttca gaaaacata 
      tcaatttat gtagacgtt tggtgttct ctgaatggg 
      aggtttcag cagcgaatg tatgacccg aactatgat 
      ttactgaag gccaattgg tcgcctttc cggccggtg 
      gagtggctg aaaccggtc ctggctgag tataatgac 
      ttcaggggg cagatgaac caaatcagt caagaggtg 
      tactcctgg tccaactac agtgatgtt ctgtttatt 
      gcagacttt cctggcctg tatttagaa aactacata 
      acgaaggat ttgagaaac gtgaccctc accgtgttg 
      gaaggtgag gtgtttttc gaaatagaa gacgaggag 
      acgagccaa tcttacgga gtaaaactc ggtaaaaac 
      gagagcgtc cccgttgaa gttgaagtg tttcataaa 
      gtgcacaca atcagttca acaccgtct tgctacatg 
      tacgtttac atgaacaag acaagggaa tatttgggg 
      gaattaccg cagtcaagc gacagcgat cgagacgtg 
      atgtattca ccgtttcct ctaatcgag gacgtgcag 
      tttcgaatc gacgccttt ttgcgaatg gttggacat 
      atttccaac tcattcttg cacgtattt tacaatcag 
      cctttaata aggaggagt agactgcca ttttcttaa
    mRNA sequence
    • >TC010953 RA
      agtcaagtc tggaacgaa aaacttccc tggagcgca 
      caacaatgg gggaacaat gtggtaaaa gtttgaaca 
      acgaattta aggaagaaa gagaaaata aaaaacaat 
      gctccaaat ttaaggaat atttaaata atgtttctt 
      atgaacagt ttgttaggt ttatgtacc agcccaagg 
      atccatcca gtctgggag ttattcgct ttttatttg 
      gtctgttaa tgttaatag acctgcctg aagaacgcg 
      gcggctctg acatcgact tcagatggg gagatcccc 
      gagactgtc atttccccc tattcccaa ttttgcgcc 
      cattgccat acccataca tggctctac tttacggaa 
      ttatgtggt ttggggctt gtggcatca tgctgggct 
      acaaatttc aaactagtg cgctgttat ttggcgttc 
      cttactggt acttattgt tactggata aatcgttct 
      ggaacaacc atagttacc ttttcggca ttgttacga 
      tacttctgg ttgggtcat cagcaaatc actacttgt 
      cgctggatg gacttttcg atgagagga aaaaaaatc 
      gccacgttc cgtactgga actatttta ttttaaaat 
      ttcagttct ttatgttgt attttttgg ctggtttaa 
      aaaaaaccg acaaggaat ggcttgagg ggtactcga 
      tgaccaact tggggaacc actgggtgt ttctcccgt 
      tcacgttat ttctcaccg ttgagcaaa tcgattatt 
      tagtagtgc attggtttg gttttctcc tcgatctca 
      caatcggtt tttggatgc tgattgaaa tcacgcgac 
      caatcgcga tgatttttt gtgcctgtt tccatttaa 
      tgaactcgc gtcttttca gcattggga tgtttccgt 
      acgtctgtc tggccacaa tgccgcttt tttgcaccg 
      aaacgtggc cccggcgct tacaaagca tttttttca 
      agaaagtaa actacagag tggagccaa gtcccgtgt 
      gcgtttaca cgagacaac aatcagaag actatcacg 
      atttacagc ttccgcacg aaatcaatt ggaaacata 
      aattagttg tggggcttt tattgaccc attgcgggc 
      tccagtgct tcctgccct attcccatt ttatcactc 
      aagggtaca acaactgga ccaagggcc tctatggct 
      actcctggg acatgatgg tgcattcct gggacacca 
      caattgtgg tggttaaag tggtcgata ataattccg 
      ggggtgagt ttttcctag accccgcag cgtggacac 
      agaatgaaa gatggaaca aacacgcgg atatgtgtc 
      tacagtacg cagagtgtt tgaaaaata atttgcttc 
      aagacttgc aacatgaga aatcgcgtg aaggtgtcg 
      aaaacgaag tcgggccta agcacatta cttcagaaa 
      acatatcaa tttatgtag acgtttggt gttctctga 
      atgggaggt ttcagcagc gaatgtatg acccgaact 
      atgatttac tgaaggcca attggtcgc ctttccggc 
      cggtggagt ggctgaaac cggtcctgg ctgagtata 
      atgacttca gggggcaga tgaaccaaa tcagtcaag 
      aggtgtact cctggtcca actacagtg atgttctgt 
      ttattgcag actttcctg gcctgtatt tagaaaact 
      acataacga aggatttga gaaacgtga ccctcaccg 
      tgttggaag gtgaggtgt ttttcgaaa tagaagacg 
      aggagacga gccaatctt acggagtaa aactcggta 
      aaaacgaga gcgtccccg ttgaagttg aagtgtttc 
      ataaagtgc acacaatca gttcaacac cgtcttgct 
      acatgtacg tttacatga acaagacaa gggaatatt 
      tgggggaat taccgcagt caagcgaca gcgatcgag 
      acgtgatgt attcaccgt ttcctctaa tcgaggacg 
      tgcagtttc gaatcgacg cctttttgc gaatggttg 
      gacatattt ccaactcat tcttgcacg tattttaca 
      atcagcctt taataagga ggagtagac tgccatttt 
      cttaataaa aaatttaca acttattgt ggaagtgtt 
      ttatttaaa ttgtccaaa tacaacatt cacaaaagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY