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Gene information
General information
TC010953 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 1919245..1927763
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC010953
    iBeetle screen iB_04946

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010953 RA
      MGEQCGKSL NNEFKEERE NKKQCSKFK EYLNNVSYE 
QFVRFMYQP KDPSSLGVI RFLFGLLML IDLPEERGG 
SDIDFRWGD PRDCHFPLF PILRPLPYP YMALLYGIM 
WFGACGIML GYKFQTSAL LFGVPYWYL LLLDKSFWN 
NHSYLFGIV TILLVGSSA NHYLSLDGL FDERKKNRH 
VPYWNYFIL KFQFFMLYF LAGLKKTDK EWLEGYSMT 
NLGNHWVFL PFTLFLTVE QIDYLVVHW FGFLLDLTI 
GFWMLIEIT RPIAMIFCA CFHLMNSRL FSIGMFPYV 
CLATMPLFC TETWPRRLQ SIFFKKVNY RVEPSPVCV 
YTRQQSEDY HDLQLPHEI NWKHKLVVG LLLTHCGLQ 
CFLPYSHFI TQGYNNWTK GLYGYSWDM MVHSWDTTI 
VVVKVVDNN SGGEFFLDP AAWTQNERW NKHADMCLQ 
YAECLKNNL LQDLQHEKS REGVENEVG PKHITSENI 
SIYVDVWCS LNGRFQQRM YDPNYDLLK ANWSPFRPV 
EWLKPVLAE YNDFRGQMN QISQEVYSW SNYSDVLFI 
ADFPGLYLE NYITKDLRN VTLTVLEGE VFFEIEDEE 
TSQSYGVKL GKNESVPVE VEVFHKVHT ISSTPSCYM 
YVYMNKTRE YLGELPQSS DSDRDVMYS PFPLIEDVQ 
FRIDAFLRM VGHISNSFL HVFYNQPLI RRSRLPFS
    CDS sequence
    • >TC010953 RA
      atgggggaa caatgtggt aaaagtttg aacaacgaa 
tttaaggaa gaaagagaa aataaaaaa caatgctcc 
aaatttaag gaatattta aataatgtt tcttatgaa 
cagtttgtt aggtttatg taccagccc aaggatcca 
tccagtctg ggagttatt cgcttttta tttggtctg 
ttaatgtta atagacctg cctgaagaa cgcggcggc 
tctgacatc gacttcaga tggggagat ccccgagac 
tgtcatttc cccctattc ccaattttg cgcccattg 
ccataccca tacatggct ctactttac ggaattatg 
tggtttggg gcttgtggc atcatgctg ggctacaaa 
tttcaaact agtgcgctg ttatttggc gttccttac 
tggtactta ttgttactg gataaatcg ttctggaac 
aaccatagt taccttttc ggcattgtt acgatactt 
ctggttggg tcatcagca aatcactac ttgtcgctg 
gatggactt ttcgatgag aggaaaaaa aatcgccac 
gttccgtac tggaactat tttatttta aaatttcag 
ttctttatg ttgtatttt ttggctggt ttaaaaaaa 
accgacaag gaatggctt gaggggtac tcgatgacc 
aacttgggg aaccactgg gtgtttctc ccgttcacg 
ttatttctc accgttgag caaatcgat tatttagta 
gtgcattgg tttggtttt ctcctcgat ctcacaatc 
ggtttttgg atgctgatt gaaatcacg cgaccaatc 
gcgatgatt ttttgtgcc tgtttccat ttaatgaac 
tcgcgtctt ttcagcatt gggatgttt ccgtacgtc 
tgtctggcc acaatgccg cttttttgc accgaaacg 
tggccccgg cgcttacaa agcattttt ttcaagaaa 
gtaaactac agagtggag ccaagtccc gtgtgcgtt 
tacacgaga caacaatca gaagactat cacgattta 
cagcttccg cacgaaatc aattggaaa cataaatta 
gttgtgggg cttttattg acccattgc gggctccag 
tgcttcctg ccctattcc cattttatc actcaaggg 
tacaacaac tggaccaag ggcctctat ggctactcc 
tgggacatg atggtgcat tcctgggac accacaatt 
gtggtggtt aaagtggtc gataataat tccgggggt 
gagtttttc ctagacccc gcagcgtgg acacagaat 
gaaagatgg aacaaacac gcggatatg tgtctacag 
tacgcagag tgtttgaaa aataatttg cttcaagac 
ttgcaacat gagaaatcg cgtgaaggt gtcgaaaac 
gaagtcggg cctaagcac attacttca gaaaacata 
tcaatttat gtagacgtt tggtgttct ctgaatggg 
aggtttcag cagcgaatg tatgacccg aactatgat 
ttactgaag gccaattgg tcgcctttc cggccggtg 
gagtggctg aaaccggtc ctggctgag tataatgac 
ttcaggggg cagatgaac caaatcagt caagaggtg 
tactcctgg tccaactac agtgatgtt ctgtttatt 
gcagacttt cctggcctg tatttagaa aactacata 
acgaaggat ttgagaaac gtgaccctc accgtgttg 
gaaggtgag gtgtttttc gaaatagaa gacgaggag 
acgagccaa tcttacgga gtaaaactc ggtaaaaac 
gagagcgtc cccgttgaa gttgaagtg tttcataaa 
gtgcacaca atcagttca acaccgtct tgctacatg 
tacgtttac atgaacaag acaagggaa tatttgggg 
gaattaccg cagtcaagc gacagcgat cgagacgtg 
atgtattca ccgtttcct ctaatcgag gacgtgcag 
tttcgaatc gacgccttt ttgcgaatg gttggacat 
atttccaac tcattcttg cacgtattt tacaatcag 
cctttaata aggaggagt agactgcca ttttcttaa
    mRNA sequence
    • >TC010953 RA
      agtcaagtc tggaacgaa aaacttccc tggagcgca 
caacaatgg gggaacaat gtggtaaaa gtttgaaca 
acgaattta aggaagaaa gagaaaata aaaaacaat 
gctccaaat ttaaggaat atttaaata atgtttctt 
atgaacagt ttgttaggt ttatgtacc agcccaagg 
atccatcca gtctgggag ttattcgct ttttatttg 
gtctgttaa tgttaatag acctgcctg aagaacgcg 
gcggctctg acatcgact tcagatggg gagatcccc 
gagactgtc atttccccc tattcccaa ttttgcgcc 
cattgccat acccataca tggctctac tttacggaa 
ttatgtggt ttggggctt gtggcatca tgctgggct 
acaaatttc aaactagtg cgctgttat ttggcgttc 
cttactggt acttattgt tactggata aatcgttct 
ggaacaacc atagttacc ttttcggca ttgttacga 
tacttctgg ttgggtcat cagcaaatc actacttgt 
cgctggatg gacttttcg atgagagga aaaaaaatc 
gccacgttc cgtactgga actatttta ttttaaaat 
ttcagttct ttatgttgt attttttgg ctggtttaa 
aaaaaaccg acaaggaat ggcttgagg ggtactcga 
tgaccaact tggggaacc actgggtgt ttctcccgt 
tcacgttat ttctcaccg ttgagcaaa tcgattatt 
tagtagtgc attggtttg gttttctcc tcgatctca 
caatcggtt tttggatgc tgattgaaa tcacgcgac 
caatcgcga tgatttttt gtgcctgtt tccatttaa 
tgaactcgc gtcttttca gcattggga tgtttccgt 
acgtctgtc tggccacaa tgccgcttt tttgcaccg 
aaacgtggc cccggcgct tacaaagca tttttttca 
agaaagtaa actacagag tggagccaa gtcccgtgt 
gcgtttaca cgagacaac aatcagaag actatcacg 
atttacagc ttccgcacg aaatcaatt ggaaacata 
aattagttg tggggcttt tattgaccc attgcgggc 
tccagtgct tcctgccct attcccatt ttatcactc 
aagggtaca acaactgga ccaagggcc tctatggct 
actcctggg acatgatgg tgcattcct gggacacca 
caattgtgg tggttaaag tggtcgata ataattccg 
ggggtgagt ttttcctag accccgcag cgtggacac 
agaatgaaa gatggaaca aacacgcgg atatgtgtc 
tacagtacg cagagtgtt tgaaaaata atttgcttc 
aagacttgc aacatgaga aatcgcgtg aaggtgtcg 
aaaacgaag tcgggccta agcacatta cttcagaaa 
acatatcaa tttatgtag acgtttggt gttctctga 
atgggaggt ttcagcagc gaatgtatg acccgaact 
atgatttac tgaaggcca attggtcgc ctttccggc 
cggtggagt ggctgaaac cggtcctgg ctgagtata 
atgacttca gggggcaga tgaaccaaa tcagtcaag 
aggtgtact cctggtcca actacagtg atgttctgt 
ttattgcag actttcctg gcctgtatt tagaaaact 
acataacga aggatttga gaaacgtga ccctcaccg 
tgttggaag gtgaggtgt ttttcgaaa tagaagacg 
aggagacga gccaatctt acggagtaa aactcggta 
aaaacgaga gcgtccccg ttgaagttg aagtgtttc 
ataaagtgc acacaatca gttcaacac cgtcttgct 
acatgtacg tttacatga acaagacaa gggaatatt 
tgggggaat taccgcagt caagcgaca gcgatcgag 
acgtgatgt attcaccgt ttcctctaa tcgaggacg 
tgcagtttc gaatcgacg cctttttgc gaatggttg 
gacatattt ccaactcat tcttgcacg tattttaca 
atcagcctt taataagga ggagtagac tgccatttt 
cttaataaa aaatttaca acttattgt ggaagtgtt 
ttatttaaa ttgtccaaa tacaacatt cacaaaagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY