Status
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Gene information
General information
TC010952 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 1894257..1913721
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010952
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04945

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010952 RA
      MAYVNVRDW SVDQVTDWL KGLDSIILQ YTTSFLNNG 
      VTGHQLLNL RADDLDNLG VKSLGHQEL ILESVEHLR 
      HFHFELDKE NLQMLALKV SCASNSLYK ELLLVDDNC 
      PIVSPQMMS DVHNIIMTI KPLVCWLDR APFLGDKDY 
      IDCKTQLLD LSFEMATRA HRGKFSEKP VSFIKDICE 
      RITKLADHI IQEISDPMV LQPASLDLA TLKKKEQEL 
      GFFISPNNH AVHQIAEIK YGSPAHGSS KIEEGDEIV 
      QVNYQTVVG WQRKNVMIL LQDSPPEIV LTLKKRPRH 
      TKVYGQIYM KPYRLPSKK LDGQTYFRW NDSIPPSRL 
      LPMHSLSPI DIPKLPVVI EPPDVEIEL SSSDDSEPP 
      DSPLDGSGR MYPLKPRPI LQRRNTITG PIPTSKTPY 
      PYMEKYWQN LKDRAVSTH SSSVNIDDT NFETDSSFI 
      RDKSASCNL GLELSPRPT TVIGIGQSR NKSFKNDFK 
      EKPAKKRVI FQEKKPEDV EKTSNLNEE KSVSVNSLA 
      SLNNTSIAF IDEGRDLTD RTSMVTEKD AALADVNIH 
      DIIKKFDEK EIVVKPKIF PRSQIKKPP DVPQKPDQS 
      KKPVVPPRS VTTKVRGRL DKSHSTPAY DLTGDENSP 
      VEKFVVKKV QTEVVEHVT STVSESEPF SEMKYELGI 
      PIVDAISLP LTQTVPVVE EIKITLTEK TEIKQGIDE 
      DLPPKPPPR TFGVVDFNK PAYPIDSPK PNISEKLKG 
      DLAAKSTVF EYLDTKHTY STEVTTVSK PPESPALLK 
      ESNKLQNKF FEPKTASTP KKSDFGESV PSFEVPKNM 
      SESKISPTN SIVRAMIYS NKNKAGKKK NTITAKRRR 
      VTVNDLNPP DTQGYLYQR LRSKHNQNV HWEKRWFVL 
      LGSCLYGFK TKEDPRAAC LIFLSGFTV AVASEVKSR 
      SNAFKVYHT GTVFYFSAE DPETLQSWI ETITAATLN 
      NDSLKTADG SLYSETDES DTEKPKKTE PEKNPETIK 
      KFNSLKKLT KKHENAAGG STSLDRKRF FNKSSSHSK 
      NSLPVPTAQ FRSYRKIKT TETPSGSVT TGNFTSHVA 
      SFFAHRSEI PQFQQSVQN VSVPNLTVE HLGKGQEKA 
      SGKLSKVKP LNYVHASNP SLCNINDFH VPNFPKKTF 
      KQCNENLAG FVTLEELMI KQSEEKKLN PYNMADDVF 
      MNLNLIKPD VVYGEVPIR PKEKVEVEE KPEPTFKKG 
      HTRNASDSQ KSSEKQDFG SSCFGKRLG SFKKIHKES 
      DDFENRTST YPKTAKGFD QKLNRSLPR THKLSENAD 
      VSRYASDRN LASERGYEM IYCPETTTD VQFAQNRNL 
      DDRMYKETP KKGHKIKKQ NSFNPSDKK TGKSGPDSA 
      KYTNKVSDS SKVKLKSAI QYTPMCLPL SQDEKSKPK 
      LAFELNLDE KSTKGGKFK QIFGKQSEG KKEKTFLGS 
      PKLHRTIFR KNNSSSELN WPSTSTQAV LPQSSYSVE 
      NSPPEPPPV SISPHADYP GLEYPPVFE PETYSLADP 
      QSSLTLLRK QGKSNN
    CDS sequence
    • >TC010952 RA
      atggcgtac gtcaacgtg agggattgg tcagttgac 
      caagtaacc gactggtta aaaggcctc gatagtatt 
      attttgcaa tatacgacc tcgttcttg aacaatggg 
      gtaacagga catcaatta cttaattta agagccgac 
      gatttggat aatttagga gttaagagt ttaggacac 
      caggaactc attttagaa tcagtggag catttgagg 
      cattttcac tttgaactg gataaagaa aatttgcaa 
      atgctggcc ttgaaggtt tcctgtgct tcgaattct 
      ttgtacaaa gagttgtta ctggttgat gataattgc 
      cccatagtt tcgccccaa atgatgtca gacgtgcac 
      aatatcata atgaccata aaaccgtta gtgtgttgg 
      ctggatcgc gcccctttt ctgggtgat aaagactac 
      attgattgt aaaacacag ttgttggat ttaagtttt 
      gaaatggcc acaagggcc caccgtgga aaattttcc 
      gaaaaacca gtgagcttt attaaggat atttgcgaa 
      cgaattacc aaacttgcc gatcatatt atacaagaa 
      atttccgat ccaatggta ttacaaccg gcatcactt 
      gatcttgca acgttaaag aaaaaagag caagagttg 
      gggtttttt atttcgcct aataatcac gctgtccat 
      cagattgct gaaataaag tatggctca ccagcgcat 
      ggaagcagc aaaattgaa gaaggggac gaaattgtg 
      caggtgaat taccagaca gttgtgggc tggcaacgc 
      aaaaatgta atgatcttg ctccaagac tcaccgcca 
      gaaattgta ctaacatta aagaaaagg ccaagacat 
      actaaagtt tacggccag atttacatg aaaccgtac 
      cgtttgcca agtaaaaag ctggacgga cagacgtac 
      ttccgctgg aatgatagc ataccgccg tccagacta 
      cttccaatg catagttta tcaccaatt gacattcca 
      aaattaccc gtagttatt gagcctcct gacgtcgaa 
      attgagtta agtagtagt gatgatagt gagccccct 
      gatagcccc ttggacggc agtggccgc atgtaccct 
      ctcaagccc aggcccatt ttgcaaagg cggaacacc 
      atcacgggg cctataccc acctccaag acgccctat 
      ccatacatg gagaagtat tggcagaac ttgaaagac 
      cgtgcggtg tcaactcac tcaagttca gttaacatt 
      gacgacaca aatttcgaa accgactcg agctttatt 
      cgggataaa agcgcttcc tgtaactta ggccttgaa 
      ttaagtcct cgaccgacg acagtcatc ggtatcggt 
      cagagccgt aacaaatcc ttcaaaaac gacttcaag 
      gaaaaaccg gccaagaaa cgggtcatt ttccaagag 
      aaaaagccc gaagatgtc gaaaagacg agtaattta 
      aacgaggag aagagcgtc agtgttaac agtttggcg 
      agtttgaat aacacgagt attgcgttc attgatgaa 
      ggacgagat ttaacagat cggacaagt atggttact 
      gagaaggat gctgcgctt gccgatgtt aatattcac 
      gatattatt aaaaagttt gatgagaag gagattgtt 
      gttaagccg aagattttt ccgagatca cagatcaag 
      aaaccacca gatgtgcca caaaaacca gaccagagt 
      aaaaaaccg gtagtgcca cctaggagt gttactacg 
      aaggttagg ggtaggttg gataaaagt cacagtacg 
      ccagcttat gatctgaca ggggatgag aattcgccg 
      gttgagaag tttgtggtg aagaaagtg caaacggaa 
      gttgttgaa cacgtaact agtacggtt tccgaaagc 
      gagccgttc tccgaaatg aaatacgaa ctcggaatc 
      cctatcgtg gacgccata agcttgcca ctgacccaa 
      acggtgcca gtcgtcgaa gaaatcaaa atcactctc 
      actgaaaaa actgaaatt aagcaagga atcgacgag 
      gatttgcca ccaaaacct ccgccacga accttcggc 
      gttgtcgat tttaacaaa ccggcttat cccatcgat 
      tcacctaaa cccaacatt tcggaaaaa ttgaaaggg 
      gatttagcg gcgaaatcg accgttttc gaatatctt 
      gacaccaaa cacacttat tcgactgaa gtaactacg 
      gtttcaaag ccacctgaa tcaccagct ttgcttaaa 
      gagagcaat aaattacaa aataagttt tttgagccg 
      aagacggca tcgactccg aaaaaatcg gactttggc 
      gaatctgtg ccaagtttt gaggtgccg aaaaatatg 
      agtgagagt aaaatttcg ccgacgaat tcgatagtg 
      cgtgccatg atttattcc aataagaat aaggcaggg 
      aaaaagaag aacacaatc actgcaaaa cggagaaga 
      gtaacagtc aacgatttg aaccctccc gacactcag 
      gggtatttg taccaacgc ttgcgaagc aaacacaac 
      caaaacgtc cactgggag aaaaggtgg tttgttttg 
      ttaggtagt tgtctgtac ggtttcaag acaaaagaa 
      gatccaaga gcggcgtgt cttattttc ctctcgggg 
      tttacggtg gctgtggcg agcgaggtg aaatctcgc 
      tcgaacgct ttcaaagtg taccacacg ggcacagta 
      ttctacttc tcagccgaa gatcctgaa accctccaa 
      tcatggatc gagacgatc accgcagcc accctcaac 
      aacgacagt ctcaaaaca gccgacggc tccctctac 
      tcagaaacg gacgaatca gacaccgaa aaacccaaa 
      aaaaccgaa ccggagaag aacccagaa actatcaaa 
      aaattcaac tctttgaag aagctcacc aagaaacac 
      gaaaacgca gccggtggc agcacgagc ttggaccgg 
      aaacgcttc ttcaataaa tcaagcagt cattcgaaa 
      aactcactt ccggtgccc accgcccag ttccgctct 
      taccgaaaa atcaaaacg acggaaact ccaagcgga 
      agtgttacc accggcaat tttacatcg catgtggcg 
      tcgtttttt gcacacagg tccgaaata ccgcagttt 
      cagcagtcg gttcagaac gtgagtgtt ccgaatttg 
      actgttgaa cacttgggt aagggtcag gaaaaggct 
      tcgggaaaa ttatccaaa gtcaaaccg ctgaattat 
      gtccatgcc agtaatcca agtttgtgc aatattaac 
      gattttcac gtcccgaat tttccgaag aagacgttc 
      aagcaatgt aatgaaaat ttggccggg tttgtgacg 
      ctggaggag ttgatgatc aagcaaagc gaggagaag 
      aaactgaat ccgtacaat atggccgat gatgttttc 
      atgaatttg aatttgatc aaaccggat gttgtctac 
      ggcgaagtc ccaatacgg cctaaagaa aaagtcgaa 
      gttgaagaa aaacccgag cctacgttc aaaaaaggc 
      cacactcgt aacgcctcc gactcgcaa aaatcgtcc 
      gaaaagcaa gactttggc agttcttgc ttcggcaaa 
      cgtctcggc tctttcaaa aaaatccat aaagaaagc 
      gacgacttt gaaaatcgc acttcaact taccctaaa 
      acagccaaa ggtttcgac cagaaatta aacagatcg 
      cttcctaga acgcacaaa ttatccgaa aatgcggac 
      gtttctcgt tacgcctcc gacagaaat ttggcaagt 
      gagagagga tacgaaatg atttattgt ccagaaacg 
      acaaccgat gtccagttt gcccaaaat cgcaatttg 
      gacgatcgc atgtacaaa gagacgccc aaaaagggc 
      cacaaaatc aaaaaacag aacagtttt aacccctcc 
      gataaaaaa acggggaaa agtgggcca gactcagca 
      aaatacacc aataaagta tccgactcg tcgaaggtt 
      aaattaaaa tcagcaatc caatacacg cccatgtgt 
      ttgccctta tcacaggac gaaaaatcc aaacccaaa 
      ctcgctttt gagctgaat ttggacgag aaaagcacc 
      aaagggggg aaattcaag cagattttc ggcaaacaa 
      agcgagggc aaaaaagag aagacgttt ttagggtcg 
      cctaaactg caccgaacc attttccgg aaaaacaat 
      tcgagttca gagctcaac tggccgtcg acttcaact 
      caggctgtt ttgccccaa agctcgtat tcggtcgag 
      aattcgccc cctgagccc ccacctgtc agtataagt 
      ccgcacgct gattatcca ggcctggaa tatccgccg 
      gtttttgag ccggaaacg tactctctt gccgaccct 
      caaagcagt ctgacattg ttgcgcaag caaggcaaa 
      agtaataat tag
    mRNA sequence
    • >TC010952 RA
      tacattggt gatattatt taggtaaat tgttaattg 
      ttgcacagt cgttgagtt gaacgagat aggtggcgc 
      cacgcgatt tcattcatc tttgacaag tagtgacaa 
      gttcattca tgtcagtca atacaaaaa acaatgcaa 
      tgaaattct gttgatatt tcttgatat ttattggtt 
      tatcgctcc acacaggcg taaatggcg tacgtcaac 
      gtgagggat tggtcagtt gaccaagta accgactgg 
      ttaaaaggc ctcgatagt attattttg caatatacg 
      acctcgttc ttgaacaat ggggtaaca ggacatcaa 
      ttacttaat ttaagagcc gacgatttg gataattta 
      ggagttaag agtttagga caccaggaa ctcatttta 
      gaatcagtg gagcatttg aggcatttt cactttgaa 
      ctggataaa gaaaatttg caaatgctg gccttgaag 
      gtttcctgt gcttcgaat tctttgtac aaagagttg 
      ttactggtt gatgataat tgccccata gtttcgccc 
      caaatgatg tcagacgtg cacaatatc ataatgacc 
      ataaaaccg ttagtgtgt tggctggat cgcgcccct 
      tttctgggt gataaagac tacattgat tgtaaaaca 
      cagttgttg gatttaagt tttgaaatg gccacaagg 
      gcccaccgt ggaaaattt tccgaaaaa ccagtgagc 
      tttattaag gatatttgc gaacgaatt accaaactt 
      gccgatcat attatacaa gaaatttcc gatccaatg 
      gtattacaa ccggcatca cttgatctt gcaacgtta 
      aagaaaaaa gagcaagag ttggggttt tttatttcg 
      cctaataat cacgctgtc catcagatt gctgaaata 
      aagtatggc tcaccagcg catggaagc agcaaaatt 
      gaagaaggg gacgaaatt gtgcaggtg aattaccag 
      acagttgtg ggctggcaa cgcaaaaat gtaatgatc 
      ttgctccaa gactcaccg ccagaaatt gtactaaca 
      ttaaagaaa aggccaaga catactaaa gtttacggc 
      cagatttac atgaaaccg taccgtttg ccaagtaaa 
      aagctggac ggacagacg tacttccgc tggaatgat 
      agcataccg ccgtccaga ctacttcca atgcatagt 
      ttatcacca attgacatt ccaaaatta cccgtagtt 
      attgagcct cctgacgtc gaaattgag ttaagtagt 
      agtgatgat agtgagccc cctgatagc cccttggac 
      ggcagtggc cgcatgtac cctctcaag cccaggccc 
      attttgcaa aggcggaac accatcacg gggcctata 
      cccacctcc aagacgccc tatccatac atggagaag 
      tattggcag aacttgaaa gaccgtgcg gtgtcaact 
      cactcaagt tcagttaac attgacgac acaaatttc 
      gaaaccgac tcgagcttt attcgggat aaaagcgct 
      tcctgtaac ttaggcctt gaattaagt cctcgaccg 
      acgacagtc atcggtatc ggtcagagc cgtaacaaa 
      tccttcaaa aacgacttc aaggaaaaa ccggccaag 
      aaacgggtc attttccaa gagaaaaag cccgaagat 
      gtcgaaaag acgagtaat ttaaacgag gagaagagc 
      gtcagtgtt aacagtttg gcgagtttg aataacacg 
      agtattgcg ttcattgat gaaggacga gatttaaca 
      gatcggaca agtatggtt actgagaag gatgctgcg 
      cttgccgat gttaatatt cacgatatt attaaaaag 
      tttgatgag aaggagatt gttgttaag ccgaagatt 
      tttccgaga tcacagatc aagaaacca ccagatgtg 
      ccacaaaaa ccagaccag agtaaaaaa ccggtagtg 
      ccacctagg agtgttact acgaaggtt aggggtagg 
      ttggataaa agtcacagt acgccagct tatgatctg 
      acaggggat gagaattcg ccggttgag aagtttgtg 
      gtgaagaaa gtgcaaacg gaagttgtt gaacacgta 
      actagtacg gtttccgaa agcgagccg ttctccgaa 
      atgaaatac gaactcgga atccctatc gtggacgcc 
      ataagcttg ccactgacc caaacggtg ccagtcgtc 
      gaagaaatc aaaatcact ctcactgaa aaaactgaa 
      attaagcaa ggaatcgac gaggatttg ccaccaaaa 
      cctccgcca cgaaccttc ggcgttgtc gattttaac 
      aaaccggct tatcccatc gattcacct aaacccaac 
      atttcggaa aaattgaaa ggggattta gcggcgaaa 
      tcgaccgtt ttcgaatat cttgacacc aaacacact 
      tattcgact gaagtaact acggtttca aagccacct 
      gaatcacca gctttgctt aaagagagc aataaatta 
      caaaataag ttttttgag ccgaagacg gcatcgact 
      ccgaaaaaa tcggacttt ggcgaatct gtgccaagt 
      tttgaggtg ccgaaaaat atgagtgag agtaaaatt 
      tcgccgacg aattcgata gtgcgtgcc atgatttat 
      tccaataag aataaggca gggaaaaag aagaacaca 
      atcactgca aaacggaga agagtaaca gtcaacgat 
      ttgaaccct cccgacact caggggtat ttgtaccaa 
      cgcttgcga agcaaacac aaccaaaac gtccactgg 
      gagaaaagg tggtttgtt ttgttaggt agttgtctg 
      tacggtttc aagacaaaa gaagatcca agagcggcg 
      tgtcttatt ttcctctcg gggtttacg gtggctgtg 
      gcgagcgag gtgaaatct cgctcgaac gctttcaaa 
      gtgtaccac acgggcaca gtattctac ttctcagcc 
      gaagatcct gaaaccctc caatcatgg atcgagacg 
      atcaccgca gccaccctc aacaacgac agtctcaaa 
      acagccgac ggctccctc tactcagaa acggacgaa 
      tcagacacc gaaaaaccc aaaaaaacc gaaccggag 
      aagaaccca gaaactatc aaaaaattc aactctttg 
      aagaagctc accaagaaa cacgaaaac gcagccggt 
      ggcagcacg agcttggac cggaaacgc ttcttcaat 
      aaatcaagc agtcattcg aaaaactca cttccggtg 
      cccaccgcc cagttccgc tcttaccga aaaatcaaa 
      acgacggaa actccaagc ggaagtgtt accaccggc 
      aattttaca tcgcatgtg gcgtcgttt tttgcacac 
      aggtccgaa ataccgcag tttcagcag tcggttcag 
      aacgtgagt gttccgaat ttgactgtt gaacacttg 
      ggtaagggt caggaaaag gcttcggga aaattatcc 
      aaagtcaaa ccgctgaat tatgtccat gccagtaat 
      ccaagtttg tgcaatatt aacgatttt cacgtcccg 
      aattttccg aagaagacg ttcaagcaa tgtaatgaa 
      aatttggcc gggtttgtg acgctggag gagttgatg 
      atcaagcaa agcgaggag aagaaactg aatccgtac 
      aatatggcc gatgatgtt ttcatgaat ttgaatttg 
      atcaaaccg gatgttgtc tacggcgaa gtcccaata 
      cggcctaaa gaaaaagtc gaagttgaa gaaaaaccc 
      gagcctacg ttcaaaaaa ggccacact cgtaacgcc 
      tccgactcg caaaaatcg tccgaaaag caagacttt 
      ggcagttct tgcttcggc aaacgtctc ggctctttc 
      aaaaaaatc cataaagaa agcgacgac tttgaaaat 
      cgcacttca acttaccct aaaacagcc aaaggtttc 
      gaccagaaa ttaaacaga tcgcttcct agaacgcac 
      aaattatcc gaaaatgcg gacgtttct cgttacgcc 
      tccgacaga aatttggca agtgagaga ggatacgaa 
      atgatttat tgtccagaa acgacaacc gatgtccag 
      tttgcccaa aatcgcaat ttggacgat cgcatgtac 
      aaagagacg cccaaaaag ggccacaaa atcaaaaaa 
      cagaacagt tttaacccc tccgataaa aaaacgggg 
      aaaagtggg ccagactca gcaaaatac accaataaa 
      gtatccgac tcgtcgaag gttaaatta aaatcagca 
      atccaatac acgcccatg tgtttgccc ttatcacag 
      gacgaaaaa tccaaaccc aaactcgct tttgagctg 
      aatttggac gagaaaagc accaaaggg gggaaattc 
      aagcagatt ttcggcaaa caaagcgag ggcaaaaaa 
      gagaagacg tttttaggg tcgcctaaa ctgcaccga 
      accattttc cggaaaaac aattcgagt tcagagctc 
      aactggccg tcgacttca actcaggct gttttgccc 
      caaagctcg tattcggtc gagaattcg ccccctgag 
      cccccacct gtcagtata agtccgcac gctgattat 
      ccaggcctg gaatatccg ccggttttt gagccggaa 
      acgtactct cttgccgac cctcaaagc agtctgaca 
      ttgttgcgc aagcaaggc aaaagtaat aattagatt 
      atttcgttt gaaaaacgt acagtcgaa atttttttg 
      atattatct tcttgtgat ttactcgat ttttgattg 
      acttggcca tagctttac agggttttg ccactttat 
      tttgtgtaa aaataaaac atagtaaga aatacttgt 
      acgcttgcc cgatattca gtctttaat agaatattt 
      aattaaatt attaataat aattgtaaa ggaaacacc 
      atttattgt tgtgatatt taaaagtgt caattacta 
      ggtacatat tgtttcaat catgatttt gtaagtagc 
      aaaaattgt taattatat tcgttttaa aggcaaacg 
      gctttttta taagtcagt tatggcaat attatatta 
      gtgttgata ttgtggttc cattagtgt ttcaggtga 
      tggaaagtt ctgttacgt aattacgtt tattaggtt 
      ttaaacttg ttattatta gatggagag attgcagta 
      ttatttgat atattttat tcatgatgt aagaaatat 
      gataattgt aacacgttt tagccaata ttttgtcca 
      ttttttacg agcattgtg tctcagggt attggctaa 
      tttaaaact gtttaactt attgatttt acaacatat 
      ctgtatata aattaattt gtaatattt gttttgctt 
      tttactatt ttaagtgcc aaattaatc acagtatga 
      aataaaatg tttgtataa aa
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