GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04942 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC033010 RA
MKNNSIHFL CFVLMASAT SATTPKSKN LISRIIGGS NAYAGQFPF AAAINVQTS DSKFFCVGT LLTNQWILT AGHCVNNAK LFTIQVGSN TLKGEDPNR VVVATSDYV LHPEFNSAT LENNIGLIK LRLPVEFTN YVQTVQHLP SAPLQVGIS VMALGWGQV NDENSDLAN DLKWVIVTS ISNNECKLT YGNQITDRM VCVAGNYNE GPCIGDSGG PIIHVVSRG QMVLVGVSS FVSGNGCES TDPSGYTRV YPYLDRGLK IRTIKQAIS LRSESSIMK LLFLVFCYF ISLISGQNP GLRIIGGDI ARASQFPYS AGIISNYPT GSFFCGGAL ISNQWILTA AQCIINATQ LTIYLGSNK IDGNDPNRV TVATATYVT HPDYNPDTL ENDVGLVRL RLPVEFNEY ISAINLPLV GLEDAAVVT AIGWGQESD HNPGLVSDL HFVEQVSIS NEECKKLYG SQIVDSMVC VVGQDIEGI CTGDSGGPL VAYRNERSA VHVAVASFY SSTGCESVY PSGYTRTYP YVDWITSVT GITP
- >TC033010 RA
atgaaaaat aattccatt cacttcctg tgctttgtt ctaatggcc tctgctacc tctgctact acgccaaaa tcaaaaaac ttgatttct cgcattatt ggtgggtct aatgcatat gcgggtcaa tttccattt gctgcagct atcaacgtc caaacttcc gacagcaag ttcttctgt gtgggaaca cttcttacc aatcagtgg attcttaca gctggtcat tgtgttaac aatgccaaa ctattcaca attcaagtt ggttcaaac actttgaaa ggtgaagac ccaaaccgt gttgttgtt gctacctca gattatgta ctccatccg gaatttaac tctgcaact ctcgaaaat aatattggt ttgatcaag cttcgactt ccagtagaa tttacaaat tatgttcaa acagttcaa catctgcca tcagcccct ttgcaagtt ggtatcagt gttatggca ctaggatgg ggccaagtt aatgatgaa aactctgat ttggccaat gacttgaaa tgggttata gtcacatca atttccaat aatgaatgt aaattaact tatggaaat caaatcaca gataggatg gtttgtgtt gctgggaat tacaacgaa ggaccttgc attggcgac agtgggggc ccgataata catgtggta agtcgagga caaatggtt cttgttgga gtttcaagt tttgtaagt ggaaatgga tgcgaaagc actgaccct tcgggatac acaagagtt tatccttat ctcgatagg ggattaaaa attaggaca ataaaacaa gcaataagc ttacgatct gaatcgtcc atcatgaag ctccttttt ctagttttt tgttatttt ataagttta atttctggg caaaatcct gggctccga attattgga ggagacatt gccagggct agtcaattt ccatactca gcaggaatt atttccaac tacccaact gggtctttt ttctgtgga ggggctctc attagtaac caatggatt ctgaccgca gcacaatgc attataaat gctacacaa ctaacgatt tatttaggt tctaataaa atagacggg aatgatcct aatcgtgtt actgtagct actgcgact tatgtaaca catccagac tataatcct gatactttg gaaaatgat gttgggcta gtcagattg cgtttgcca gttgaattt aatgaatac atatcagcg ataaatttg cctttggtc gggcttgaa gacgcagct gttgttaca gcaatcgga tggggacaa gagagtgac cacaacccg ggtcttgta agtgaccta cacttcgta gaacaagtc tcgatttcg aacgaagag tgtaagaag ctttatggt agtcaaatt gtggatagt atggtttgt gtcgttggt caagatatt gaagggatt tgtaccggt gatagtggt ggaccattg gtggcttac agaaatgaa cgatcggct gtgcatgtt gctgtagct tctttttat agcagtact ggatgcgaa agtgtttat ccgtctggt tataccaga acttatcca tatgtggat tggatcact agtgtcaca ggaataaca ccttaa
- >TC033010 RA
aaataaaac gattagata aatatcagt cttttgagc aactatcct ctcagtgtt tctttttat tgcaaagac aatcctcgt atcgtaaaa aatgaaaaa taattccat tcacttcct gtgctttgt tctaatggc ctctgctac ctctgctac tacgccaaa atcaaaaaa cttgatttc tcgcattat tggtgggtc taatgcata tgcgggtca atttccatt tgctgcagc tatcaacgt ccaaacttc cgacagcaa gttcttctg tgtgggaac acttcttac caatcagtg gattcttac agctggtca ttgtgttaa caatgccaa actattcac aattcaagt tggttcaaa cactttgaa aggtgaaga cccaaaccg tgttgttgt tgctacctc agattatgt actccatcc ggaatttaa ctctgcaac tctcgaaaa taatattgg tttgatcaa gcttcgact tccagtaga atttacaaa ttatgttca aacagttca acatctgcc atcagcccc tttgcaagt tggtatcag tgttatggc actaggatg gggccaagt taatgatga aaactctga tttggccaa tgacttgaa atgggttat agtcacatc aatttccaa taatgaatg taaattaac ttatggaaa tcaaatcac agataggat ggtttgtgt tgctgggaa ttacaacga aggaccttg cattggcga cagtggggg cccgataat acatgtggt aagtcgagg acaaatggt tcttgttgg agtttcaag ttttgtaag tggaaatgg atgcgaaag cactgaccc ttcgggata cacaagagt ttatcctta tctcgatag gggattaaa aattaggac aataaaaca agcaataag cttacgatc tgaatcgtc catcatgaa gctcctttt tctagtttt ttgttattt tataagttt aatttctgg gcaaaatcc tgggctccg aattattgg aggagacat tgccagggc tagtcaatt tccatactc agcaggaat tatttccaa ctacccaac tgggtcttt tttctgtgg aggggctct cattagtaa ccaatggat tctgaccgc agcacaatg cattataaa tgctacaca actaacgat ttatttagg ttctaataa aatagacgg gaatgatcc taatcgtgt tactgtagc tactgcgac ttatgtaac acatccaga ctataatcc tgatacttt ggaaaatga tgttgggct agtcagatt gcgtttgcc agttgaatt taatgaata catatcagc gataaattt gcctttggt cgggcttga agacgcagc tgttgttac agcaatcgg atggggaca agagagtga ccacaaccc gggtcttgt aagtgacct acacttcgt agaacaagt ctcgatttc gaacgaaga gtgtaagaa gctttatgg tagtcaaat tgtggatag tatggtttg tgtcgttgg tcaagatat tgaagggat ttgtaccgg tgatagtgg tggaccatt ggtggctta cagaaatga acgatcggc tgtgcatgt tgctgtagc ttcttttta tagcagtac tggatgcga aagtgttta tccgtctgg ttataccag aacttatcc atatgtgga ttggatcac tagtgtcac aggaataac accttaatt ttaattttg gtattcaat aaaaaaaaa ttaagata
