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Gene information
General information
TC033010
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010941
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 1548516..1554444
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033010
    iBeetle screen iB_04942

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033010 RA
      MKNNSIHFL CFVLMASAT SATTPKSKN LISRIIGGS 
      NAYAGQFPF AAAINVQTS DSKFFCVGT LLTNQWILT 
      AGHCVNNAK LFTIQVGSN TLKGEDPNR VVVATSDYV 
      LHPEFNSAT LENNIGLIK LRLPVEFTN YVQTVQHLP 
      SAPLQVGIS VMALGWGQV NDENSDLAN DLKWVIVTS 
      ISNNECKLT YGNQITDRM VCVAGNYNE GPCIGDSGG 
      PIIHVVSRG QMVLVGVSS FVSGNGCES TDPSGYTRV 
      YPYLDRGLK IRTIKQAIS LRSESSIMK LLFLVFCYF 
      ISLISGQNP GLRIIGGDI ARASQFPYS AGIISNYPT 
      GSFFCGGAL ISNQWILTA AQCIINATQ LTIYLGSNK 
      IDGNDPNRV TVATATYVT HPDYNPDTL ENDVGLVRL 
      RLPVEFNEY ISAINLPLV GLEDAAVVT AIGWGQESD 
      HNPGLVSDL HFVEQVSIS NEECKKLYG SQIVDSMVC 
      VVGQDIEGI CTGDSGGPL VAYRNERSA VHVAVASFY 
      SSTGCESVY PSGYTRTYP YVDWITSVT GITP
    CDS sequence
    • >TC033010 RA
      atgaaaaat aattccatt cacttcctg tgctttgtt 
      ctaatggcc tctgctacc tctgctact acgccaaaa 
      tcaaaaaac ttgatttct cgcattatt ggtgggtct 
      aatgcatat gcgggtcaa tttccattt gctgcagct 
      atcaacgtc caaacttcc gacagcaag ttcttctgt 
      gtgggaaca cttcttacc aatcagtgg attcttaca 
      gctggtcat tgtgttaac aatgccaaa ctattcaca 
      attcaagtt ggttcaaac actttgaaa ggtgaagac 
      ccaaaccgt gttgttgtt gctacctca gattatgta 
      ctccatccg gaatttaac tctgcaact ctcgaaaat 
      aatattggt ttgatcaag cttcgactt ccagtagaa 
      tttacaaat tatgttcaa acagttcaa catctgcca 
      tcagcccct ttgcaagtt ggtatcagt gttatggca 
      ctaggatgg ggccaagtt aatgatgaa aactctgat 
      ttggccaat gacttgaaa tgggttata gtcacatca 
      atttccaat aatgaatgt aaattaact tatggaaat 
      caaatcaca gataggatg gtttgtgtt gctgggaat 
      tacaacgaa ggaccttgc attggcgac agtgggggc 
      ccgataata catgtggta agtcgagga caaatggtt 
      cttgttgga gtttcaagt tttgtaagt ggaaatgga 
      tgcgaaagc actgaccct tcgggatac acaagagtt 
      tatccttat ctcgatagg ggattaaaa attaggaca 
      ataaaacaa gcaataagc ttacgatct gaatcgtcc 
      atcatgaag ctccttttt ctagttttt tgttatttt 
      ataagttta atttctggg caaaatcct gggctccga 
      attattgga ggagacatt gccagggct agtcaattt 
      ccatactca gcaggaatt atttccaac tacccaact 
      gggtctttt ttctgtgga ggggctctc attagtaac 
      caatggatt ctgaccgca gcacaatgc attataaat 
      gctacacaa ctaacgatt tatttaggt tctaataaa 
      atagacggg aatgatcct aatcgtgtt actgtagct 
      actgcgact tatgtaaca catccagac tataatcct 
      gatactttg gaaaatgat gttgggcta gtcagattg 
      cgtttgcca gttgaattt aatgaatac atatcagcg 
      ataaatttg cctttggtc gggcttgaa gacgcagct 
      gttgttaca gcaatcgga tggggacaa gagagtgac 
      cacaacccg ggtcttgta agtgaccta cacttcgta 
      gaacaagtc tcgatttcg aacgaagag tgtaagaag 
      ctttatggt agtcaaatt gtggatagt atggtttgt 
      gtcgttggt caagatatt gaagggatt tgtaccggt 
      gatagtggt ggaccattg gtggcttac agaaatgaa 
      cgatcggct gtgcatgtt gctgtagct tctttttat 
      agcagtact ggatgcgaa agtgtttat ccgtctggt 
      tataccaga acttatcca tatgtggat tggatcact 
      agtgtcaca ggaataaca ccttaa
    mRNA sequence
    • >TC033010 RA
      aaataaaac gattagata aatatcagt cttttgagc 
      aactatcct ctcagtgtt tctttttat tgcaaagac 
      aatcctcgt atcgtaaaa aatgaaaaa taattccat 
      tcacttcct gtgctttgt tctaatggc ctctgctac 
      ctctgctac tacgccaaa atcaaaaaa cttgatttc 
      tcgcattat tggtgggtc taatgcata tgcgggtca 
      atttccatt tgctgcagc tatcaacgt ccaaacttc 
      cgacagcaa gttcttctg tgtgggaac acttcttac 
      caatcagtg gattcttac agctggtca ttgtgttaa 
      caatgccaa actattcac aattcaagt tggttcaaa 
      cactttgaa aggtgaaga cccaaaccg tgttgttgt 
      tgctacctc agattatgt actccatcc ggaatttaa 
      ctctgcaac tctcgaaaa taatattgg tttgatcaa 
      gcttcgact tccagtaga atttacaaa ttatgttca 
      aacagttca acatctgcc atcagcccc tttgcaagt 
      tggtatcag tgttatggc actaggatg gggccaagt 
      taatgatga aaactctga tttggccaa tgacttgaa 
      atgggttat agtcacatc aatttccaa taatgaatg 
      taaattaac ttatggaaa tcaaatcac agataggat 
      ggtttgtgt tgctgggaa ttacaacga aggaccttg 
      cattggcga cagtggggg cccgataat acatgtggt 
      aagtcgagg acaaatggt tcttgttgg agtttcaag 
      ttttgtaag tggaaatgg atgcgaaag cactgaccc 
      ttcgggata cacaagagt ttatcctta tctcgatag 
      gggattaaa aattaggac aataaaaca agcaataag 
      cttacgatc tgaatcgtc catcatgaa gctcctttt 
      tctagtttt ttgttattt tataagttt aatttctgg 
      gcaaaatcc tgggctccg aattattgg aggagacat 
      tgccagggc tagtcaatt tccatactc agcaggaat 
      tatttccaa ctacccaac tgggtcttt tttctgtgg 
      aggggctct cattagtaa ccaatggat tctgaccgc 
      agcacaatg cattataaa tgctacaca actaacgat 
      ttatttagg ttctaataa aatagacgg gaatgatcc 
      taatcgtgt tactgtagc tactgcgac ttatgtaac 
      acatccaga ctataatcc tgatacttt ggaaaatga 
      tgttgggct agtcagatt gcgtttgcc agttgaatt 
      taatgaata catatcagc gataaattt gcctttggt 
      cgggcttga agacgcagc tgttgttac agcaatcgg 
      atggggaca agagagtga ccacaaccc gggtcttgt 
      aagtgacct acacttcgt agaacaagt ctcgatttc 
      gaacgaaga gtgtaagaa gctttatgg tagtcaaat 
      tgtggatag tatggtttg tgtcgttgg tcaagatat 
      tgaagggat ttgtaccgg tgatagtgg tggaccatt 
      ggtggctta cagaaatga acgatcggc tgtgcatgt 
      tgctgtagc ttcttttta tagcagtac tggatgcga 
      aagtgttta tccgtctgg ttataccag aacttatcc 
      atatgtgga ttggatcac tagtgtcac aggaataac 
      accttaatt ttaattttg gtattcaat aaaaaaaaa 
      ttaagata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY