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Gene information
General information
TC010931 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 1449819..1455134
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010931
    iBeetle screen iB_04939

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature partially shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    mandible not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    mandible potentially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    legs partiallydecreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010931 RA
      MSCCANEPY IGPPLDRTC FGGSYIVFM HLLNTLITQ 
      QCDVSEICQ RINPQLQPD SEYDFVVIG GGAGGSVVA 
      GRLSENPNW KILLIEAGG DEPPGSQVP SMMNNYLGD 
      SQMDWRYRT EPQEMACLG RPGRRCDWP RGRVLGGSG 
      VIHGMMYMR GLPSDYNEW EARGNEGWG YKDVEEYFK 
      KSEGNRDIG DGVEGRYHS SDGPMLVQR FPDQPQIAE 
      DVLRAGAEL GYPVVGDLN GEQHWGFTI AQANIKNGS 
      RLSSARAFL RPARNRPNL HVMINSTAT KILINSNDT 
      AKTISAVEF TYNNQSFTV KVRREAIVS AGAINTPHL 
      LLLSGIGPR EELDKVGIE QVHNLPGVG QNLKNHVSF 
      AVNFQLTKI ENYNDLNWN TVREYLTER RGPMSSTGV 
      TQVAARISS KYANPDGKN PDLQFFFSG FLAHCSLSG 
      GVKEPEDPT NPTAAKSFT IRPTFLRPR SRGFIGLNS 
      RDPKEPPLM QPNYLTDEE DVKRMVAGI RIAQNLANT 
      TILTTKYGI QMVNTDYGD CSRNYTFDS DEFWACALR 
      YDTGPENHQ SCSCKMGPA SDPSAVVDP KLQVHGIEG 
      LRIMDASVM PTVLSGNTH ATVVMIAEK GSDYIKQKW 
      SDK
    CDS sequence
    • >TC010931 RA
      atgtcttgt tgcgccaac gagccctac atcggccca 
      cccctagac cggacctgt ttcgggggc tcctacatc 
      gtcttcatg cacctcctc aacaccctc atcacccaa 
      cagtgcgac gtatccgaa atctgccaa cgaatcaac 
      ccacaactc caacctgac tccgaatac gacttcgtg 
      gtgatcgga ggtggagct gggggttct gtggtggcg 
      ggacgttta tcagaaaac cccaactgg aaaattctc 
      ctcatcgaa gccggcggc gatgaacct ccaggttca 
      caagtcccc tccatgatg aataactac ctgggggat 
      tcacaaatg gattggaga tatcggacg gagccccag 
      gaaatggcc tgtcttggg aggcctggg cggaggtgc 
      gattggccc agagggagg gttttgggg ggcagtggc 
      gtcatacat gggatgatg tacatgcgg gggcttcca 
      agtgactac aacgagtgg gaagcgaga gggaatgag 
      ggttggggt tacaaggac gtggaggag tacttcaag 
      aagtcggaa gggaaccgg gatattggt gatggggtt 
      gaagggagg tatcatagc tctgatggt cccatgttg 
      gtccagagg tttcccgat cagccccaa attgctgaa 
      gatgttttg agggctggg gcggagttg gggtatcca 
      gtggttggg gatttgaac ggggagcag cattggggg 
      tttaccatt gcacaagca aatattaag aatggatcg 
      agattaagc agtgccaga gctttcctg cgtccagct 
      cgcaatcgt ccaaactta cacgtgatg atcaattca 
      acagccacc aaaatcctg atcaatagt aacgacaca 
      gcaaaaaca atctcagca gtagaattt acgtacaat 
      aaccagtct tttaccgta aaagtgaga cgcgaagct 
      atcgtatca gctggtgca atcaacaca cctcacctc 
      cttttacta tctggaata ggccctaga gaagaacta 
      gacaaagtg gggatcgaa caggtccat aatttgcca 
      ggtgttggc caaaacctc aaaaaccac gtatcgttt 
      gcggtaaat ttccaattg acaaaaatc gaaaattat 
      aacgacttg aactggaac actgtgagg gagtatttg 
      acggagaga agaggcccc atgtcttcg accggagtc 
      acacaagtc gctgccaga attagctcg aaatatgcc 
      aatcctgat ggcaaaaat cccgacttg caattcttc 
      ttttcgggc tttttggcg cattgttcg ctgtcggga 
      ggagtcaag gaaccagaa gaccccaca aatcccaca 
      gctgcgaaa agttttacc ataagaccg acttttcta 
      aggccaaga agtcgcggt tttatcgga ctgaattcc 
      agagatcca aaggagcca cctctcatg caaccaaat 
      tacttgact gatgaggaa gacgtcaaa cgaatggtt 
      gcaggaatt cggattgct caaaatttg gcaaacact 
      acaatcctt actacaaag tatggtata caaatggtg 
      aacactgat tatggagac tgttctaga aactacact 
      ttcgactcg gatgaattc tgggcatgt gctttgagg 
      tatgatacg ggtccagaa aatcaccag tcttgttcc 
      tgcaaaatg ggtccggct tcggaccca tcggcggta 
      gtggatccg aaattgcaa gttcatggt attgaaggt 
      ctcaggatt atggatgcg tcagtgatg cctacagtt 
      ctctcggga aatacgcat gcaaccgtt gtgatgatt 
      gctgagaaa ggttctgat tatatcaag cagaaatgg 
      agtgataaa taa
    mRNA sequence
    • >TC010931 RA
      gttgaaaat gtcttgttg cgccaacga gccctacat 
      cggcccacc cctagaccg gacctgttt cgggggctc 
      ctacatcgt cttcatgca cctcctcaa caccctcat 
      cacccaaca gtgcgacgt atccgaaat ctgccaacg 
      aatcaaccc acaactcca acctgactc cgaatacga 
      cttcgtggt gatcggagg tggagctgg gggttctgt 
      ggtggcggg acgtttatc agaaaaccc caactggaa 
      aattctcct catcgaagc cggcggcga tgaacctcc 
      aggttcaca agtcccctc catgatgaa taactacct 
      gggggattc acaaatgga ttggagata tcggacgga 
      gccccagga aatggcctg tcttgggag gcctgggcg 
      gaggtgcga ttggcccag agggagggt tttgggggg 
      cagtggcgt catacatgg gatgatgta catgcgggg 
      gcttccaag tgactacaa cgagtggga agcgagagg 
      gaatgaggg ttggggtta caaggacgt ggaggagta 
      cttcaagaa gtcggaagg gaaccggga tattggtga 
      tggggttga agggaggta tcatagctc tgatggtcc 
      catgttggt ccagaggtt tcccgatca gccccaaat 
      tgctgaaga tgttttgag ggctggggc ggagttggg 
      gtatccagt ggttgggga tttgaacgg ggagcagca 
      ttgggggtt taccattgc acaagcaaa tattaagaa 
      tggatcgag attaagcag tgccagagc tttcctgcg 
      tccagctcg caatcgtcc aaacttaca cgtgatgat 
      caattcaac agccaccaa aatcctgat caatagtaa 
      cgacacagc aaaaacaat ctcagcagt agaatttac 
      gtacaataa ccagtcttt taccgtaaa agtgagacg 
      cgaagctat cgtatcagc tggtgcaat caacacacc 
      tcacctcct tttactatc tggaatagg ccctagaga 
      agaactaga caaagtggg gatcgaaca ggtccataa 
      tttgccagg tgttggcca aaacctcaa aaaccacgt 
      atcgtttgc ggtaaattt ccaattgac aaaaatcga 
      aaattataa cgacttgaa ctggaacac tgtgaggga 
      gtatttgac ggagagaag aggccccat gtcttcgac 
      cggagtcac acaagtcgc tgccagaat tagctcgaa 
      atatgccaa tcctgatgg caaaaatcc cgacttgca 
      attcttctt ttcgggctt tttggcgca ttgttcgct 
      gtcgggagg agtcaagga accagaaga ccccacaaa 
      tcccacagc tgcgaaaag ttttaccat aagaccgac 
      ttttctaag gccaagaag tcgcggttt tatcggact 
      gaattccag agatccaaa ggagccacc tctcatgca 
      accaaatta cttgactga tgaggaaga cgtcaaacg 
      aatggttgc aggaattcg gattgctca aaatttggc 
      aaacactac aatccttac tacaaagta tggtataca 
      aatggtgaa cactgatta tggagactg ttctagaaa 
      ctacacttt cgactcgga tgaattctg ggcatgtgc 
      tttgaggta tgatacggg tccagaaaa tcaccagtc 
      ttgttcctg caaaatggg tccggcttc ggacccatc 
      ggcggtagt ggatccgaa attgcaagt tcatggtat 
      tgaaggtct caggattat ggatgcgtc agtgatgcc 
      tacagttct ctcgggaaa tacgcatgc aaccgttgt 
      gatgattgc tgagaaagg ttctgatta tatcaagca 
      gaaatggag tgataaata aaattttaa atcaaatgt 
      ttattttaa tttgtagtt atagttgat tgcaaaact 
      aactccttt tttattaca ataaaaaag ttgtaaaaa 
      a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY