General information | |||
TC010931 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 1449819..1455134 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC010931 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_04939 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
larval musculature partially shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
mandible not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
mandible potentially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 50% - 80%
legs partiallydecreased - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC010931 RA
MSCCANEPY IGPPLDRTC FGGSYIVFM HLLNTLITQ QCDVSEICQ RINPQLQPD SEYDFVVIG GGAGGSVVA GRLSENPNW KILLIEAGG DEPPGSQVP SMMNNYLGD SQMDWRYRT EPQEMACLG RPGRRCDWP RGRVLGGSG VIHGMMYMR GLPSDYNEW EARGNEGWG YKDVEEYFK KSEGNRDIG DGVEGRYHS SDGPMLVQR FPDQPQIAE DVLRAGAEL GYPVVGDLN GEQHWGFTI AQANIKNGS RLSSARAFL RPARNRPNL HVMINSTAT KILINSNDT AKTISAVEF TYNNQSFTV KVRREAIVS AGAINTPHL LLLSGIGPR EELDKVGIE QVHNLPGVG QNLKNHVSF AVNFQLTKI ENYNDLNWN TVREYLTER RGPMSSTGV TQVAARISS KYANPDGKN PDLQFFFSG FLAHCSLSG GVKEPEDPT NPTAAKSFT IRPTFLRPR SRGFIGLNS RDPKEPPLM QPNYLTDEE DVKRMVAGI RIAQNLANT TILTTKYGI QMVNTDYGD CSRNYTFDS DEFWACALR YDTGPENHQ SCSCKMGPA SDPSAVVDP KLQVHGIEG LRIMDASVM PTVLSGNTH ATVVMIAEK GSDYIKQKW SDK
- >TC010931 RA
atgtcttgt tgcgccaac gagccctac atcggccca cccctagac cggacctgt ttcgggggc tcctacatc gtcttcatg cacctcctc aacaccctc atcacccaa cagtgcgac gtatccgaa atctgccaa cgaatcaac ccacaactc caacctgac tccgaatac gacttcgtg gtgatcgga ggtggagct gggggttct gtggtggcg ggacgttta tcagaaaac cccaactgg aaaattctc ctcatcgaa gccggcggc gatgaacct ccaggttca caagtcccc tccatgatg aataactac ctgggggat tcacaaatg gattggaga tatcggacg gagccccag gaaatggcc tgtcttggg aggcctggg cggaggtgc gattggccc agagggagg gttttgggg ggcagtggc gtcatacat gggatgatg tacatgcgg gggcttcca agtgactac aacgagtgg gaagcgaga gggaatgag ggttggggt tacaaggac gtggaggag tacttcaag aagtcggaa gggaaccgg gatattggt gatggggtt gaagggagg tatcatagc tctgatggt cccatgttg gtccagagg tttcccgat cagccccaa attgctgaa gatgttttg agggctggg gcggagttg gggtatcca gtggttggg gatttgaac ggggagcag cattggggg tttaccatt gcacaagca aatattaag aatggatcg agattaagc agtgccaga gctttcctg cgtccagct cgcaatcgt ccaaactta cacgtgatg atcaattca acagccacc aaaatcctg atcaatagt aacgacaca gcaaaaaca atctcagca gtagaattt acgtacaat aaccagtct tttaccgta aaagtgaga cgcgaagct atcgtatca gctggtgca atcaacaca cctcacctc cttttacta tctggaata ggccctaga gaagaacta gacaaagtg gggatcgaa caggtccat aatttgcca ggtgttggc caaaacctc aaaaaccac gtatcgttt gcggtaaat ttccaattg acaaaaatc gaaaattat aacgacttg aactggaac actgtgagg gagtatttg acggagaga agaggcccc atgtcttcg accggagtc acacaagtc gctgccaga attagctcg aaatatgcc aatcctgat ggcaaaaat cccgacttg caattcttc ttttcgggc tttttggcg cattgttcg ctgtcggga ggagtcaag gaaccagaa gaccccaca aatcccaca gctgcgaaa agttttacc ataagaccg acttttcta aggccaaga agtcgcggt tttatcgga ctgaattcc agagatcca aaggagcca cctctcatg caaccaaat tacttgact gatgaggaa gacgtcaaa cgaatggtt gcaggaatt cggattgct caaaatttg gcaaacact acaatcctt actacaaag tatggtata caaatggtg aacactgat tatggagac tgttctaga aactacact ttcgactcg gatgaattc tgggcatgt gctttgagg tatgatacg ggtccagaa aatcaccag tcttgttcc tgcaaaatg ggtccggct tcggaccca tcggcggta gtggatccg aaattgcaa gttcatggt attgaaggt ctcaggatt atggatgcg tcagtgatg cctacagtt ctctcggga aatacgcat gcaaccgtt gtgatgatt gctgagaaa ggttctgat tatatcaag cagaaatgg agtgataaa taa
- >TC010931 RA
gttgaaaat gtcttgttg cgccaacga gccctacat cggcccacc cctagaccg gacctgttt cgggggctc ctacatcgt cttcatgca cctcctcaa caccctcat cacccaaca gtgcgacgt atccgaaat ctgccaacg aatcaaccc acaactcca acctgactc cgaatacga cttcgtggt gatcggagg tggagctgg gggttctgt ggtggcggg acgtttatc agaaaaccc caactggaa aattctcct catcgaagc cggcggcga tgaacctcc aggttcaca agtcccctc catgatgaa taactacct gggggattc acaaatgga ttggagata tcggacgga gccccagga aatggcctg tcttgggag gcctgggcg gaggtgcga ttggcccag agggagggt tttgggggg cagtggcgt catacatgg gatgatgta catgcgggg gcttccaag tgactacaa cgagtggga agcgagagg gaatgaggg ttggggtta caaggacgt ggaggagta cttcaagaa gtcggaagg gaaccggga tattggtga tggggttga agggaggta tcatagctc tgatggtcc catgttggt ccagaggtt tcccgatca gccccaaat tgctgaaga tgttttgag ggctggggc ggagttggg gtatccagt ggttgggga tttgaacgg ggagcagca ttgggggtt taccattgc acaagcaaa tattaagaa tggatcgag attaagcag tgccagagc tttcctgcg tccagctcg caatcgtcc aaacttaca cgtgatgat caattcaac agccaccaa aatcctgat caatagtaa cgacacagc aaaaacaat ctcagcagt agaatttac gtacaataa ccagtcttt taccgtaaa agtgagacg cgaagctat cgtatcagc tggtgcaat caacacacc tcacctcct tttactatc tggaatagg ccctagaga agaactaga caaagtggg gatcgaaca ggtccataa tttgccagg tgttggcca aaacctcaa aaaccacgt atcgtttgc ggtaaattt ccaattgac aaaaatcga aaattataa cgacttgaa ctggaacac tgtgaggga gtatttgac ggagagaag aggccccat gtcttcgac cggagtcac acaagtcgc tgccagaat tagctcgaa atatgccaa tcctgatgg caaaaatcc cgacttgca attcttctt ttcgggctt tttggcgca ttgttcgct gtcgggagg agtcaagga accagaaga ccccacaaa tcccacagc tgcgaaaag ttttaccat aagaccgac ttttctaag gccaagaag tcgcggttt tatcggact gaattccag agatccaaa ggagccacc tctcatgca accaaatta cttgactga tgaggaaga cgtcaaacg aatggttgc aggaattcg gattgctca aaatttggc aaacactac aatccttac tacaaagta tggtataca aatggtgaa cactgatta tggagactg ttctagaaa ctacacttt cgactcgga tgaattctg ggcatgtgc tttgaggta tgatacggg tccagaaaa tcaccagtc ttgttcctg caaaatggg tccggcttc ggacccatc ggcggtagt ggatccgaa attgcaagt tcatggtat tgaaggtct caggattat ggatgcgtc agtgatgcc tacagttct ctcgggaaa tacgcatgc aaccgttgt gatgattgc tgagaaagg ttctgatta tatcaagca gaaatggag tgataaata aaattttaa atcaaatgt ttattttaa tttgtagtt atagttgat tgcaaaact aactccttt tttattaca ataaaaaag ttgtaaaaa a
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
