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Gene information
General information
TC010931 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 1449819..1455134
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC010931
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04939

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature partially shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     mandible not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    mandible potentially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    legs partiallydecreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010931 RA
      MSCCANEPY IGPPLDRTC FGGSYIVFM HLLNTLITQ 
QCDVSEICQ RINPQLQPD SEYDFVVIG GGAGGSVVA 
GRLSENPNW KILLIEAGG DEPPGSQVP SMMNNYLGD 
SQMDWRYRT EPQEMACLG RPGRRCDWP RGRVLGGSG 
VIHGMMYMR GLPSDYNEW EARGNEGWG YKDVEEYFK 
KSEGNRDIG DGVEGRYHS SDGPMLVQR FPDQPQIAE 
DVLRAGAEL GYPVVGDLN GEQHWGFTI AQANIKNGS 
RLSSARAFL RPARNRPNL HVMINSTAT KILINSNDT 
AKTISAVEF TYNNQSFTV KVRREAIVS AGAINTPHL 
LLLSGIGPR EELDKVGIE QVHNLPGVG QNLKNHVSF 
AVNFQLTKI ENYNDLNWN TVREYLTER RGPMSSTGV 
TQVAARISS KYANPDGKN PDLQFFFSG FLAHCSLSG 
GVKEPEDPT NPTAAKSFT IRPTFLRPR SRGFIGLNS 
RDPKEPPLM QPNYLTDEE DVKRMVAGI RIAQNLANT 
TILTTKYGI QMVNTDYGD CSRNYTFDS DEFWACALR 
YDTGPENHQ SCSCKMGPA SDPSAVVDP KLQVHGIEG 
LRIMDASVM PTVLSGNTH ATVVMIAEK GSDYIKQKW 
SDK
    CDS sequence
    • >TC010931 RA
      atgtcttgt tgcgccaac gagccctac atcggccca 
cccctagac cggacctgt ttcgggggc tcctacatc 
gtcttcatg cacctcctc aacaccctc atcacccaa 
cagtgcgac gtatccgaa atctgccaa cgaatcaac 
ccacaactc caacctgac tccgaatac gacttcgtg 
gtgatcgga ggtggagct gggggttct gtggtggcg 
ggacgttta tcagaaaac cccaactgg aaaattctc 
ctcatcgaa gccggcggc gatgaacct ccaggttca 
caagtcccc tccatgatg aataactac ctgggggat 
tcacaaatg gattggaga tatcggacg gagccccag 
gaaatggcc tgtcttggg aggcctggg cggaggtgc 
gattggccc agagggagg gttttgggg ggcagtggc 
gtcatacat gggatgatg tacatgcgg gggcttcca 
agtgactac aacgagtgg gaagcgaga gggaatgag 
ggttggggt tacaaggac gtggaggag tacttcaag 
aagtcggaa gggaaccgg gatattggt gatggggtt 
gaagggagg tatcatagc tctgatggt cccatgttg 
gtccagagg tttcccgat cagccccaa attgctgaa 
gatgttttg agggctggg gcggagttg gggtatcca 
gtggttggg gatttgaac ggggagcag cattggggg 
tttaccatt gcacaagca aatattaag aatggatcg 
agattaagc agtgccaga gctttcctg cgtccagct 
cgcaatcgt ccaaactta cacgtgatg atcaattca 
acagccacc aaaatcctg atcaatagt aacgacaca 
gcaaaaaca atctcagca gtagaattt acgtacaat 
aaccagtct tttaccgta aaagtgaga cgcgaagct 
atcgtatca gctggtgca atcaacaca cctcacctc 
cttttacta tctggaata ggccctaga gaagaacta 
gacaaagtg gggatcgaa caggtccat aatttgcca 
ggtgttggc caaaacctc aaaaaccac gtatcgttt 
gcggtaaat ttccaattg acaaaaatc gaaaattat 
aacgacttg aactggaac actgtgagg gagtatttg 
acggagaga agaggcccc atgtcttcg accggagtc 
acacaagtc gctgccaga attagctcg aaatatgcc 
aatcctgat ggcaaaaat cccgacttg caattcttc 
ttttcgggc tttttggcg cattgttcg ctgtcggga 
ggagtcaag gaaccagaa gaccccaca aatcccaca 
gctgcgaaa agttttacc ataagaccg acttttcta 
aggccaaga agtcgcggt tttatcgga ctgaattcc 
agagatcca aaggagcca cctctcatg caaccaaat 
tacttgact gatgaggaa gacgtcaaa cgaatggtt 
gcaggaatt cggattgct caaaatttg gcaaacact 
acaatcctt actacaaag tatggtata caaatggtg 
aacactgat tatggagac tgttctaga aactacact 
ttcgactcg gatgaattc tgggcatgt gctttgagg 
tatgatacg ggtccagaa aatcaccag tcttgttcc 
tgcaaaatg ggtccggct tcggaccca tcggcggta 
gtggatccg aaattgcaa gttcatggt attgaaggt 
ctcaggatt atggatgcg tcagtgatg cctacagtt 
ctctcggga aatacgcat gcaaccgtt gtgatgatt 
gctgagaaa ggttctgat tatatcaag cagaaatgg 
agtgataaa taa
    mRNA sequence
    • >TC010931 RA
      gttgaaaat gtcttgttg cgccaacga gccctacat 
cggcccacc cctagaccg gacctgttt cgggggctc 
ctacatcgt cttcatgca cctcctcaa caccctcat 
cacccaaca gtgcgacgt atccgaaat ctgccaacg 
aatcaaccc acaactcca acctgactc cgaatacga 
cttcgtggt gatcggagg tggagctgg gggttctgt 
ggtggcggg acgtttatc agaaaaccc caactggaa 
aattctcct catcgaagc cggcggcga tgaacctcc 
aggttcaca agtcccctc catgatgaa taactacct 
gggggattc acaaatgga ttggagata tcggacgga 
gccccagga aatggcctg tcttgggag gcctgggcg 
gaggtgcga ttggcccag agggagggt tttgggggg 
cagtggcgt catacatgg gatgatgta catgcgggg 
gcttccaag tgactacaa cgagtggga agcgagagg 
gaatgaggg ttggggtta caaggacgt ggaggagta 
cttcaagaa gtcggaagg gaaccggga tattggtga 
tggggttga agggaggta tcatagctc tgatggtcc 
catgttggt ccagaggtt tcccgatca gccccaaat 
tgctgaaga tgttttgag ggctggggc ggagttggg 
gtatccagt ggttgggga tttgaacgg ggagcagca 
ttgggggtt taccattgc acaagcaaa tattaagaa 
tggatcgag attaagcag tgccagagc tttcctgcg 
tccagctcg caatcgtcc aaacttaca cgtgatgat 
caattcaac agccaccaa aatcctgat caatagtaa 
cgacacagc aaaaacaat ctcagcagt agaatttac 
gtacaataa ccagtcttt taccgtaaa agtgagacg 
cgaagctat cgtatcagc tggtgcaat caacacacc 
tcacctcct tttactatc tggaatagg ccctagaga 
agaactaga caaagtggg gatcgaaca ggtccataa 
tttgccagg tgttggcca aaacctcaa aaaccacgt 
atcgtttgc ggtaaattt ccaattgac aaaaatcga 
aaattataa cgacttgaa ctggaacac tgtgaggga 
gtatttgac ggagagaag aggccccat gtcttcgac 
cggagtcac acaagtcgc tgccagaat tagctcgaa 
atatgccaa tcctgatgg caaaaatcc cgacttgca 
attcttctt ttcgggctt tttggcgca ttgttcgct 
gtcgggagg agtcaagga accagaaga ccccacaaa 
tcccacagc tgcgaaaag ttttaccat aagaccgac 
ttttctaag gccaagaag tcgcggttt tatcggact 
gaattccag agatccaaa ggagccacc tctcatgca 
accaaatta cttgactga tgaggaaga cgtcaaacg 
aatggttgc aggaattcg gattgctca aaatttggc 
aaacactac aatccttac tacaaagta tggtataca 
aatggtgaa cactgatta tggagactg ttctagaaa 
ctacacttt cgactcgga tgaattctg ggcatgtgc 
tttgaggta tgatacggg tccagaaaa tcaccagtc 
ttgttcctg caaaatggg tccggcttc ggacccatc 
ggcggtagt ggatccgaa attgcaagt tcatggtat 
tgaaggtct caggattat ggatgcgtc agtgatgcc 
tacagttct ctcgggaaa tacgcatgc aaccgttgt 
gatgattgc tgagaaagg ttctgatta tatcaagca 
gaaatggag tgataaata aaattttaa atcaaatgt 
ttattttaa tttgtagtt atagttgat tgcaaaact 
aactccttt tttattaca ataaaaaag ttgtaaaaa 
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