Status
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Gene information
General information
TC010916 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 1307884..1319168
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010916
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04936

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010916 RA
      MMECLNELL MHAIIQNDL KCLKCYLKR GVNINKICS 
      TGMTLLGVA AETGNLSIV KTLIDYSNC SSIDFSIKT 
      ELNTKPNLL LDLNNPEPK YKNIGYFVV RKDVEEPDF 
      GEGPTPEGM EALEWDMEV NTETTEVEK EPPEMNIYQ 
      WYANILNQS SLVLKSPDN DIARLDSHG KNVLHYAVR 
      SGNMELVKY LVETFNEIS VNQSDAASV SPLHIAVFK 
      CHLPLVRYL VDKGASVNC ACRERQTPL HVAAQHGYV 
      EIMQVLIEN EADINVFDM KERSPLSLA VLGSREAAV 
      ELLIKNGAR LNQEDAAGF TPLRRAVWN DSTPIAMML 
      LNAGARVVQ SHYLLHAAA RSNNPEMVK ALHEAGALL 
      NIRDDQGNT PLMVACSRK NLTVARYLL KNGANVNAV 
      NNINGMTAL HICVQDIRE KRSLENLID LLVSYDADM 
      NATSYQGSV LFYSIILEN LHGADALVR HGVDVNLRD 
      ERAYFDNLS LAKRHGNLE LVKLIVYAG FRLSNMAFD 
      LNSLRNQPD DPVHDFLVY VKSTPLQLK EICRIKIRG 
      CLGRHLLSK ITKLPLPVW LQRYLALEI L
    CDS sequence
    • >TC010916 RA
      atgatggaa tgtttaaac gaattgttg atgcatgcc 
      atcattcag aacgactta aaatgcctc aagtgctac 
      ttaaagaga ggtgtaaac atcaacaaa atctgttca 
      actggaatg accctcctg ggagttgca gctgagaca 
      ggcaatctc tcaatagtc aaaacacta attgactat 
      tccaattgc tccagtatt gatttcagt ataaaaact 
      gagctaaac acaaaacca aacctatta ctagattta 
      aataacccg gaaccaaag tacaaaaac atcggatat 
      tttgtggtg cgcaaggac gtggaggag cccgatttt 
      ggcgagggc cccaccccc gagggcatg gaagccctg 
      gaatgggac atggaagtg aacacagaa accacagag 
      gttgagaaa gagccccca gaaatgaac atctaccag 
      tggtacgcc aacattttg aatcagagc tctttggtc 
      ttgaaatcg ccggataac gatattgcg cgccttgac 
      agccacggc aaaaacgtc ttgcattac gccgtgagg 
      tcaggtaat atggaacta gtcaaatat ttagtcgag 
      accttcaac gaaatcagc gtgaatcaa agcgacgcc 
      gcctccgtg agccccctt cacatcgcc gtgttcaag 
      tgccacctg ccgctggtg cgctacctg gtggacaag 
      ggggcttcc gtgaactgc gcgtgtcgc gagcgtcag 
      acgcccctg cacgtggcc gcccagcat ggctacgtc 
      gaaatcatg caagtttta atcgaaaat gaagccgat 
      atcaacgtt tttgatatg aaagagcgg tcaccgctc 
      tcgctggct gtgctgggc tctagggaa gctgcagtg 
      gaattgttg ataaaaaac ggggccaga cttaatcag 
      gaggatgcg gctggattt acgcccttg cgaagagcg 
      gtctggaat gattccaca ccgatcgcg atgatgtta 
      cttaacgct ggagcgaga gttgtgcag tcacattat 
      ttattacat gccgctgcg aggagtaat aatccggag 
      atggtcaag gcgttgcat gaagctggg gcgcttttg 
      aatatcagg gatgatcaa gggaatacg cccttgatg 
      gtggcgtgc tcgaggaag aatttgacg gtggcgaga 
      tatctactc aaaaatggt gctaacgta aacgccgta 
      aacaacatc aacggtatg acggccctc cacatctgc 
      gtgcaagat atccgcgaa aagcgaagt ctggagaac 
      ttaatcgac cttttggtc tcctacgac gccgacatg 
      aacgcaaca agttatcag ggaagtgtt ctcttctat 
      tcaattatt ttagaaaac ctccatgga gccgatgcc 
      ttagtgcgg cacggagtc gacgtcaat ttacgcgac 
      gaacgcgct tatttcgat aatttaagt cttgcgaaa 
      agacacgga aacctcgaa ttggtcaaa ttgatagtt 
      tacgctggt tttcgccta tctaacatg gcctttgat 
      ttgaattcg ttgcggaat caacccgac gatcccgta 
      cacgatttt ttggtttac gtcaaaagc acaccttta 
      caattgaag gaaatatgc aggataaaa atcagaggc 
      tgtctcggg cgacatctt ctgagcaaa atcaccaag 
      ctgccgttg cccgtgtgg ctacagcgg tacttggct 
      ttagaaata ttgtga
    mRNA sequence
    • >TC010916 RA
      tgtcaaata aacgtcaaa aaagtaaat aattgacaa 
      tggcgcgtc aaaacaatg agaacgttt tttaatgtt 
      tacaatgaa ttccatcac cgaaacgca ataacttta 
      ggccgccac cgtcgagtt ccaatcatg ttcaagatc 
      agatgatac aaacttacg aaaaaaatg atggaatgt 
      ttaaacgaa ttgttgatg catgccatc attcagaac 
      gacttaaaa tgcctcaag tgctactta aagagaggt 
      gtaaacatc aacaaaatc tgttcaact ggaatgacc 
      ctcctggga gttgcagct gagacaggc aatctctca 
      atagtcaaa acactaatt gactattcc aattgctcc 
      agtattgat ttcagtata aaaactgag ctaaacaca 
      aaaccaaac ctattacta gatttaaat aacccggaa 
      ccaaagtac aaaaacatc ggatatttt gtggtgcgc 
      aaggacgtg gaggagccc gattttggc gagggcccc 
      acccccgag ggcatggaa gccctggaa tgggacatg 
      gaagtgaac acagaaacc acagaggtt gagaaagag 
      cccccagaa atgaacatc taccagtgg tacgccaac 
      attttgaat cagagctct ttggtcttg aaatcgccg 
      gataacgat attgcgcgc cttgacagc cacggcaaa 
      aacgtcttg cattacgcc gtgaggtca ggtaatatg 
      gaactagtc aaatattta gtcgagacc ttcaacgaa 
      atcagcgtg aatcaaagc gacgccgcc tccgtgagc 
      ccccttcac atcgccgtg ttcaagtgc cacctgccg 
      ctggtgcgc tacctggtg gacaagggg gcttccgtg 
      aactgcgcg tgtcgcgag cgtcagacg cccctgcac 
      gtggccgcc cagcatggc tacgtcgaa atcatgcaa 
      gttttaatc gaaaatgaa gccgatatc aacgttttt 
      gatatgaaa gagcggtca ccgctctcg ctggctgtg 
      ctgggctct agggaagct gcagtggaa ttgttgata 
      aaaaacggg gccagactt aatcaggag gatgcggct 
      ggatttacg cccttgcga agagcggtc tggaatgat 
      tccacaccg atcgcgatg atgttactt aacgctgga 
      gcgagagtt gtgcagtca cattattta ttacatgcc 
      gctgcgagg agtaataat ccggagatg gtcaaggcg 
      ttgcatgaa gctggggcg cttttgaat atcagggat 
      gatcaaggg aatacgccc ttgatggtg gcgtgctcg 
      aggaagaat ttgacggtg gcgagatat ctactcaaa 
      aatggtgct aacgtaaac gccgtaaac aacatcaac 
      ggtatgacg gccctccac atctgcgtg caagatatc 
      cgcgaaaag cgaagtctg gagaactta atcgacctt 
      ttggtctcc tacgacgcc gacatgaac gcaacaagt 
      tatcaggga agtgttctc ttctattca attatttta 
      gaaaacctc catggagcc gatgcctta gtgcggcac 
      ggagtcgac gtcaattta cgcgacgaa cgcgcttat 
      ttcgataat ttaagtctt gcgaaaaga cacggaaac 
      ctcgaattg gtcaaattg atagtttac gctggtttt 
      cgcctatct aacatggcc tttgatttg aattcgttg 
      cggaatcaa cccgacgat cccgtacac gattttttg 
      gtttacgtc aaaagcaca cctttacaa ttgaaggaa 
      atatgcagg ataaaaatc agaggctgt ctcgggcga 
      catcttctg agcaaaatc accaagctg ccgttgccc 
      gtgtggcta cagcggtac ttggcttta gaaatattg 
      tgaatgatc tctgtgttt atgtaacca gtattcaac 
      aagtgtgta ggtttcagt attcaaatc gtagatttt 
      caagagagg accatcggt gtgttgccg ttgatagaa 
      acaagtagt gcccttaaa tgtttgatg agtaagaga 
      ggtttaaaa atctgatga atataaatt ttaagtatt 
      gtgcaccta tttctaaac cacctgtgt acttagatg 
      actttagca ttccctcct ctgttatag tctagtcaa 
      tttatttaa caccatgct ttgaaatta agttggcaa 
      cattaactg ctaaataca aacgtcaca atttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY