Status
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Gene information
General information
TC033009
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010902,TC010903
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 1562074..1570774
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033009
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04934

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 7 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033009 RA
      MSVVKSPAQ IEDLPDEVL EFILSLIPP YKDLHDCMQ 
      VSKRWRRCV LNVAKTKLR NLHKAIVDF DIRWFTLTP 
      VEMAPTISK RYSHTAAIH ENSMYVFGG CTCSMTTFN 
      DLWRLDLSK RQWVRPLAM GTYPSPKAC SSLICYKDL 
      LVLFGGWAY PPSYPLYQS WHLFNELHI YDIAANHWT 
      CVNTTNTPP PTAGHSVSV VGEWMIVFG GIHKPSTAV 
      HCEKSNDIW KLNLETWTW YRQEVEGEP RPNGRLGQT 
      QVVLDPKHL LIIGGSGGP TLNYCDAWI LNMEGAVWK 
      WKQVDIEGK ANKPNNIWT NPGCKIGDK IVMLNRIRE 
      DQTCPIVYY PKNSWVNRR SPQEDSKLA RIDLASREP 
      DRDENVNGR RGVLRNQKR EADDDDVYQ PGCSNNQRG 
      DILINNWSP PINNLAAFS VPNNRLSKS DRIREQRLA 
      RLSEIKENM KKNKKEEKN KSHYLGIYV LDLSKALSS 
      RPYVTWLPP KNLGNGPEE TILYTLLAG KSELVMFGG 
      IRKDPTSLG FSVNFNSQI SNSLHFISA PNYVI
    CDS sequence
    • >TC033009 RA
      atgtctgtg gtgaaaagt ccggctcaa attgaagac 
      ctccccgac gaggttttg gaatttatt ttatctcta 
      attccaccc tataaagac ctgcacgat tgcatgcaa 
      gtgtcgaaa agatggaga agatgtgtt ttaaatgtt 
      gccaaaacc aaattgagg aatctgcat aaggccatt 
      gttgatttc gatataaga tggttcact ctgaccccc 
      gtggagatg gcccccacg atatcgaag cgttattcg 
      cacacagcc gccattcat gaaaattcg atgtacgtt 
      ttcgggggc tgcacctgc tccatgacc acgttcaac 
      gacttgtgg aggctagat ttgtccaaa cggcaatgg 
      gtgcggccc ctggccatg ggcacgtac ccttcgccc 
      aaagcctgc agctctcta atctgttat aaggacctt 
      ttagtatta ttcggggga tgggcgtac ccaccttcg 
      tacccccta taccaaagt tggcacctc ttcaacgag 
      ctgcacatc tacgacatc gcagcaaat cactggact 
      tgtgtcaac accaccaac acgccgcct cccaccgcc 
      ggccattcc gtatcagtt gtaggcgaa tggatgatc 
      gtttttggc ggcattcat aaaccgagc actgccgtt 
      cattgtgaa aaatccaac gatatttgg aagctaaat 
      ttagaaacg tggacgtgg tatagacag gaagttgaa 
      ggcgaaccg cgaccaaat ggcagatta ggacaaact 
      caagtagtt ttagatccg aaacacttg ttgataatt 
      gggggctca ggagggccc actcttaat tactgtgat 
      gcttggatt ttgaatatg gagggggcg gtgtggaag 
      tggaagcag gtggatatt gaagggaag gccaacaag 
      cctaataat atctggacc aaccctggc tgtaagata 
      ggggacaaa attgtgatg ctcaaccgc attcgcgaa 
      gaccaaact tgcccaata gtttactac ccaaagaat 
      tcctgggtg aaccgtaga agcccacaa gaagatagt 
      aaattagct aggatagat ttagcaagt cgcgaacca 
      gacagggat gagaacgtc aacggaaga cgcggagtt 
      ttaaggaac cagaagaga gaagctgac gatgacgac 
      gtctaccaa cctggctgt tcgaacaac caacgagga 
      gatattcta atcaacaac tggtcgcca cccatcaac 
      aatttagct gcatttagc gtcccaaac aacagattg 
      tcgaagtcg gacaggata cgggagcaa cgtctagca 
      cgcttgtca gaaattaaa gaaaatatg aagaaaaat 
      aaaaaagaa gagaagaac aagagtcac tatttaggg 
      atttacgtt ttagatctt tccaaagct ttaagttcg 
      agaccatat gtgacatgg ttgccaccc aaaaatttg 
      ggtaacgga cctgaagaa acgattttg tatactttg 
      ctggctggg aagtctgaa ctggtcatg tttggagga 
      attagaaag gatcccact tctttaggg ttttcggtt 
      aatttcaac agtcaaatt tccaacagt ttacatttt 
      atttctgca cccaattat gttatctga
    mRNA sequence
    • >TC033009 RA
      ggtgagaaa tgtcaacgt ctcgatgac attttacga 
      aaatatcaa caaaaactt ggaattttc gggtttatt 
      tttattttg tcgtagaac cttcgatga gagcagttg 
      ataagtgtg aaaattgaa gtgttttat aaaatgtct 
      gtggtgaaa agtccggct caaattgaa gacctcccc 
      gacgaggtt ttggaattt attttatct ctaattcca 
      ccctataaa gacctgcac gattgcatg caagtgtcg 
      aaaagatgg agaagatgt gttttaaat gttgccaaa 
      accaaattg aggaatctg cataaggcc attgttgat 
      ttcgatata agatggttc actctgacc cccgtggag 
      atggccccc acgatatcg aagcgttat tcgcacaca 
      gccgccatt catgaaaat tcgatgtac gttttcggg 
      ggctgcacc tgctccatg accacgttc aacgacttg 
      tggaggcta gatttgtcc aaacggcaa tgggtgcgg 
      cccctggcc atgggcacg tacccttcg cccaaagcc 
      tgcagctct ctaatctgt tataaggac cttttagta 
      ttattcggg ggatgggcg tacccacct tcgtacccc 
      ctataccaa agttggcac ctcttcaac gagctgcac 
      atctacgac atcgcagca aatcactgg acttgtgtc 
      aacaccacc aacacgccg cctcccacc gccggccat 
      tccgtatca gttgtaggc gaatggatg atcgttttt 
      ggcggcatt cataaaccg agcactgcc gttcattgt 
      gaaaaatcc aacgatatt tggaagcta aatttagaa 
      acgtggacg tggtataga caggaagtt gaaggcgaa 
      ccgcgacca aatggcaga ttaggacaa actcaagta 
      gttttagat ccgaaacac ttgttgata attgggggc 
      tcaggaggg cccactctt aattactgt gatgcttgg 
      attttgaat atggagggg gcggtgtgg aagtggaag 
      caggtggat attgaaggg aaggccaac aagcctaat 
      aatatctgg accaaccct ggctgtaag ataggggac 
      aaaattgtg atgctcaac cgcattcgc gaagaccaa 
      acttgccca atagtttac tacccaaag aattcctgg 
      gtgaaccgt agaagccca caagaagat agtaaatta 
      gctaggata gatttagca agtcgcgaa ccagacagg 
      gatgagaac gtcaacgga agacgcgga gttttaagg 
      aaccagaag agagaagct gacgatgac gacgtctac 
      caacctggc tgttcgaac aaccaacga ggagatatt 
      ctaatcaac aactggtcg ccacccatc aacaattta 
      gctgcattt agcgtccca aacaacaga ttgtcgaag 
      tcggacagg atacgggag caacgtcta gcacgcttg 
      tcagaaatt aaagaaaat atgaagaaa aataaaaaa 
      gaagagaag aacaagagt cactattta gggatttac 
      gttttagat ctttccaaa gctttaagt tcgagacca 
      tatgtgaca tggttgcca cccaaaaat ttgggtaac 
      ggacctgaa gaaacgatt ttgtatact ttgctggct 
      gggaagtct gaactggtc atgtttgga ggaattaga 
      aaggatccc acttcttta gggttttcg gttaatttc 
      aacagtcaa atttccaac agtttacat tttatttct 
      gcacccaat tatgttatc tgaacgaat tatattatt 
      ttttctgca attctagca ttactaggg agaatgtgc 
      tagtcattg aatgattgc tgatttttt tttgagtta 
      ttggagaat tatattgta tcgttccgt ttgattatt 
      ctgaaatac attttttat tattactaa cattgtttt 
      atgtacccc tatagtacc tgagtaaaa aaaaaaacg 
      ttaaaaaaa taatagacc gtatgaata aagttaagt 
      aaatgtt
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