Status
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Gene information
General information
TC010900 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 1650105..1655589
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010900
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04933

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 8 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010900 RA
      MASSSRYDT NKVCCNSHP DAPLIEDYR AGDQICSEC 
      GLVVGDRVI DVGSEWRTF SNEKSGVDP SRVGGPENP 
      LLNGSDLTT MIGPGRGDA SFDSFGVAR YQNRKTMSS 
      SDRALINAF REINGMADR INLPRTIVD RANNLFKQV 
      HDGRNLKGR SNDAIASAC LYIACRQEG VPRTFKEIC 
      AVSKISKRE IGRCFKLIL KALETSVDL ITTGDFMSR 
      FCSNLGLPN MVQRAATHI ARKAVELDI VPGRSPISV 
      AAAAIYMAS QASEDKRSQ KEIGDIAGV ADVTIRQSY 
      KLMYPHAVK LFPEDFKFA TPIDQLPQM
    CDS sequence
    • >TC010900 RA
      atggcgagt agttcaagg tatgacacg aataaggtt 
      tgttgtaat tctcatcca gatgccccc cttattgaa 
      gattacaga gcaggcgat cagatttgc tccgaatgc 
      ggtttagta gtaggtgac agagtaata gacgttgga 
      tctgaatgg agaaccttc agcaacgaa aaatcgggg 
      gtcgatcct tctcgtgtt ggtggcccc gagaacccc 
      cttcttaac ggctcagac ctgaccaca atgataggc 
      cccggtcga ggggacgcc tcctttgat agtttcggt 
      gtcgcgcgc taccagaac cgtaaaacc atgagcagt 
      tccgaccgc gctttgatc aacgctttt cgtgaaatc 
      aacggaatg gcggatcgc attaatctt ccaaggact 
      atagtagac cgtgctaat aacttgttc aaacaagtg 
      catgatgga cgtaacttg aaaggacgc tctaacgac 
      gccatagcg tcggcgtgt ctttacata gcttgtcgg 
      caagagggc gtccctcga acgttcaaa gaaatctgc 
      gccgtcagt aaaattagt aaacgagag attggaaga 
      tgttttaaa ttgattttg aaagcgctt gaaacttca 
      gtggatctc attaccact ggtgatttt atgtcgagg 
      ttttgctcg aaccttggt ttgccgaat atggtgcaa 
      agggcggct acgcatatt gcaaggaag gcggtggag 
      ttggatatt gttccaggg agatcgccg atttctgta 
      gctgcagct gctatttac atggcgtca caggcttcg 
      gaagacaaa cgaagtcag aaggaaatc ggcgacatc 
      gcgggggtg gccgacgtc accattcgc cagtcctac 
      aagttgatg tacccccac gctgtgaaa ctatttcca 
      gaagatttc aaattcgca acgccaatc gatcagtta 
      ccccaaatg taa
    mRNA sequence
    • >TC010900 RA
      cagtgtcga ttaactatc acacttgtc aaaacttga 
      aaattgtca taataacaa taaatagag aagaaatag 
      tgcaaaatt gtacaagat ggcgagtag ttcaaggta 
      tgacacgaa taaggtttg ttgtaattc tcatccaga 
      tgcccccct tattgaaga ttacagagc aggcgatca 
      gatttgctc cgaatgcgg tttagtagt aggtgacag 
      agtaataga cgttggatc tgaatggag aaccttcag 
      caacgaaaa atcgggggt cgatccttc tcgtgttgg 
      tggccccga gaaccccct tcttaacgg ctcagacct 
      gaccacaat gataggccc cggtcgagg ggacgcctc 
      ctttgatag tttcggtgt cgcgcgcta ccagaaccg 
      taaaaccat gagcagttc cgaccgcgc tttgatcaa 
      cgcttttcg tgaaatcaa cggaatggc ggatcgcat 
      taatcttcc aaggactat agtagaccg tgctaataa 
      cttgttcaa acaagtgca tgatggacg taacttgaa 
      aggacgctc taacgacgc catagcgtc ggcgtgtct 
      ttacatagc ttgtcggca agagggcgt ccctcgaac 
      gttcaaaga aatctgcgc cgtcagtaa aattagtaa 
      acgagagat tggaagatg ttttaaatt gattttgaa 
      agcgcttga aacttcagt ggatctcat taccactgg 
      tgattttat gtcgaggtt ttgctcgaa ccttggttt 
      gccgaatat ggtgcaaag ggcggctac gcatattgc 
      aaggaaggc ggtggagtt ggatattgt tccagggag 
      atcgccgat ttctgtagc tgcagctgc tatttacat 
      ggcgtcaca ggcttcgga agacaaacg aagtcagaa 
      ggaaatcgg cgacatcgc gggggtggc cgacgtcac 
      cattcgcca gtcctacaa gttgatgta cccccacgc 
      tgtgaaact atttccaga agatttcaa attcgcaac 
      gccaatcga tcagttacc ccaaatgta aattcggat 
      ccttgtcac attttgttt gtctttttt atggaaaaa 
      ttgatgtaa attttattt ataggtttt aagcactag 
      gtcgagtta ggtattttt tacaaaaac acattaggt 
      acaataatt gatctt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY