Status
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Gene information
General information
TC010898 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 1710318..1732029
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010898
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04932

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010898 RA
      MVRFSLRSV LGVAVLLVV GFSETQSQF SYVCPRKFV 
      AIGRKCYYF SKDETIWHD AHFKCRNLN SSLATIKGP 
      QQELSVRRY LNRRNDGRF ERWLGGRYN EKQSRWVWG 
      ENGKPLVFH GFSRMNAND RKWAWHCII VDPTRQNKW 
      SARSCVERK RYICQRAVI GKRNIIRRQ CTSAANNTS 
      SQGCLTPKV GSSAPAFLI RKPQKPTKP YHVTTFVCP 
      SHMILVGRK CYFFSMDKA SWSNAYWAC RDNKTKLAV 
      ITTKMQDNI LRGFLKWNF TAGNHERWL GGIFDWKQQ 
      KWKWAMSGK AIRYKNFAK EALVGQKNN THHCITIDP 
      YYNNLWNSK NRLEEKHYI CQAKSKSVV KYDKLWDHN 
      ASVVIQPEG QEEFISKKS KQNFAATSS SPLDFNNEV 
      TDKYVYN
    • >TC010898 RB
      MVRFSLRSV LGVAVLLVV GFSETQSQF SYVCPRKFV 
      AIGRKCYYF SKDETIWHD AHFKCRNLN SSLATIKGP 
      QQELSVRRY LNRRNDGRF ERWLGGRYN EKQSRWVWG 
      ENGKPLVFH GFSRMNAND RKWAWHCII VDPTRQNKW 
      SARSCVERK RYICQRAVI GKRNIIRRQ CTSAANNTS 
      SQGCLTPKV GSSAPAFLI RKPQKPTKP YHVTTFVCP 
      SHMILVGRK CYFFSMDKA SWSNAYWAC RDNKTKLAV 
      ITTKMQDNI LRGFLKWNF TGNHERWLG GIFDWKQQK 
      WKWAMSGKA IRYKNFAKE ALVGQKNNT HHCITIDPY 
      YNNLWNSKN RLEEKHYIC QAKSKSVVK YDKLWDHNA 
      SVVIQPEGQ EEFISKKSK QNFAATSSS PLDFNNEVT 
      DKYVYN
    CDS sequence
    • >TC010898 RA
      atggtgaga tttagtctg agaagcgtc ttgggcgtc 
      gcggtgctg ctcgtagtt ggtttttca gaaacacaa 
      agccagttt agttacgtc tgccccaga aaatttgtg 
      gcgatcgga agaaaatgt tattacttc agcaaggac 
      gaaactatt tggcacgac gcccacttt aaatgtaga 
      aacctcaac agtagtttg gccacaatt aagggaccc 
      cagcaagaa ctgagcgtg agaagatat ctaaaccga 
      cgaaatgac gggaggttt gagaggtgg ttgggagga 
      cgctacaat gagaaacag tccaggtgg gtttggggt 
      gaaaatgga aaacctctg gttttccac ggattttct 
      aggatgaat gctaatgac aggaagtgg gcatggcat 
      tgcattatt gtggatccc actcgacaa aacaaatgg 
      tcggcaagg agttgtgtt gagaggaag cgctacatc 
      tgccaaagg gccgttata ggtaaaagg aatattatc 
      aggaggcag tgtacttca gcggcgaat aacaccagc 
      agccagggt tgtttgacg cctaaagtt ggatcctca 
      gctcctgca tttttaatc agaaaacct caaaagcca 
      actaaaccg taccatgta accacattc gtctgtcca 
      tctcacatg atcctggtg ggccgaaag tgctacttc 
      ttcagcatg gacaaagcc agctggagc aacgcttac 
      tgggcttgc agagacaac aaaaccaaa ctcgcagtc 
      atcacaacc aaaatgcag gacaatatt ttgagagga 
      tttctcaaa tggaacttt actgcagga aaccacgaa 
      cgctggctt ggtggaatt tttgattgg aagcagcaa 
      aaatggaag tgggcgatg agtggaaaa gcgatcaga 
      tacaaaaat tttgccaaa gaagctcta gtgggacag 
      aaaaataac actcaccat tgtatcaca atcgaccct 
      tattataac aacttatgg aactcgaaa aataggctg 
      gaagagaaa cactacatt tgtcaagct aaatcgaaa 
      agtgtggta aaatatgat aaattgtgg gaccataat 
      gcttcagtt gtcatccaa cctgagggc caggaggaa 
      tttattagt aaaaaatct aaacaaaat tttgctgcc 
      acatctagt tcgccacta gattttaat aatgaagtc 
      acggataag tacgtttat aattaa
    • >TC010898 RB
      atggtgaga tttagtctg agaagcgtc ttgggcgtc 
      gcggtgctg ctcgtagtt ggtttttca gaaacacaa 
      agccagttt agttacgtc tgccccaga aaatttgtg 
      gcgatcgga agaaaatgt tattacttc agcaaggac 
      gaaactatt tggcacgac gcccacttt aaatgtaga 
      aacctcaac agtagtttg gccacaatt aagggaccc 
      cagcaagaa ctgagcgtg agaagatat ctaaaccga 
      cgaaatgac gggaggttt gagaggtgg ttgggagga 
      cgctacaat gagaaacag tccaggtgg gtttggggt 
      gaaaatgga aaacctctg gttttccac ggattttct 
      aggatgaat gctaatgac aggaagtgg gcatggcat 
      tgcattatt gtggatccc actcgacaa aacaaatgg 
      tcggcaagg agttgtgtt gagaggaag cgctacatc 
      tgccaaagg gccgttata ggtaaaagg aatattatc 
      aggaggcag tgtacttca gcggcgaat aacaccagc 
      agccagggt tgtttgacg cctaaagtt ggatcctca 
      gctcctgca tttttaatc agaaaacct caaaagcca 
      actaaaccg taccatgta accacattc gtctgtcca 
      tctcacatg atcctggtg ggccgaaag tgctacttc 
      ttcagcatg gacaaagcc agctggagc aacgcttac 
      tgggcttgc agagacaac aaaaccaaa ctcgcagtc 
      atcacaacc aaaatgcag gacaatatt ttgagagga 
      tttctcaaa tggaacttt actggaaac cacgaacgc 
      tggcttggt ggaattttt gattggaag cagcaaaaa 
      tggaagtgg gcgatgagt ggaaaagcg atcagatac 
      aaaaatttt gccaaagaa gctctagtg ggacagaaa 
      aataacact caccattgt atcacaatc gacccttat 
      tataacaac ttatggaac tcgaaaaat aggctggaa 
      gagaaacac tacatttgt caagctaaa tcgaaaagt 
      gtggtaaaa tatgataaa ttgtgggac cataatgct 
      tcagttgtc atccaacct gagggccag gaggaattt 
      attagtaaa aaatctaaa caaaatttt gctgccaca 
      tctagttcg ccactagat tttaataat gaagtcacg 
      gataagtac gtttataat taa
    mRNA sequence
    • >TC010898 RA
      tcgctctaa aaaactcaa aaagtgctg ttgcaagtg 
      tgttgtgaa ttttcattt tagtgattt ttttgaaca 
      gaatcgata aaaccgtgc aatcgtcgg aacgttgtt 
      gattactac cctgcatcc gataagcat caagcaatt 
      taaaaaacg caacatgaa ttacattta aatatagaa 
      aagtgtctt tcaacgatg tgaaatccg agtgacttt 
      gaattacta aaaatgtgc aaatttaac atttagttg 
      ggatggtga gatttagtc tgagaagcg tcttgggcg 
      tcgcggtgc tgctcgtag ttggttttt cagaaacac 
      aaagccagt ttagttacg tctgcccca gaaaatttg 
      tggcgatcg gaagaaaat gttattact tcagcaagg 
      acgaaacta tttggcacg acgcccact ttaaatgta 
      gaaacctca acagtagtt tggccacaa ttaagggac 
      cccagcaag aactgagcg tgagaagat atctaaacc 
      gacgaaatg acgggaggt ttgagaggt ggttgggag 
      gacgctaca atgagaaac agtccaggt gggtttggg 
      gtgaaaatg gaaaacctc tggttttcc acggatttt 
      ctaggatga atgctaatg acaggaagt gggcatggc 
      attgcatta ttgtggatc ccactcgac aaaacaaat 
      ggtcggcaa ggagttgtg ttgagagga agcgctaca 
      tctgccaaa gggccgtta taggtaaaa ggaatatta 
      tcaggaggc agtgtactt cagcggcga ataacacca 
      gcagccagg gttgtttga cgcctaaag ttggatcct 
      cagctcctg catttttaa tcagaaaac ctcaaaagc 
      caactaaac cgtaccatg taaccacat tcgtctgtc 
      catctcaca tgatcctgg tgggccgaa agtgctact 
      tcttcagca tggacaaag ccagctgga gcaacgctt 
      actgggctt gcagagaca acaaaacca aactcgcag 
      tcatcacaa ccaaaatgc aggacaata ttttgagag 
      gatttctca aatggaact ttactgcag gaaaccacg 
      aacgctggc ttggtggaa tttttgatt ggaagcagc 
      aaaaatgga agtgggcga tgagtggaa aagcgatca 
      gatacaaaa attttgcca aagaagctc tagtgggac 
      agaaaaata acactcacc attgtatca caatcgacc 
      cttattata acaacttat ggaactcga aaaataggc 
      tggaagaga aacactaca tttgtcaag ctaaatcga 
      aaagtgtgg taaaatatg ataaattgt gggaccata 
      atgcttcag ttgtcatcc aacctgagg gccaggagg 
      aatttatta gtaaaaaat ctaaacaaa attttgctg 
      ccacatcta gttcgccac tagatttta ataatgaag 
      tcacggata agtacgttt ataattaaa tatacctat 
      acaatttta gtgcctaga acatcattc ttgaatctc 
      ccacatatt caatcaaca gacaaaatg taaatagta 
      tttttcaat atttaataa gttagtaat acatatttg 
      tagcttta
    • >TC010898 RB
      tcgctctaa aaaactcaa aaagtgctg ttgcaagtg 
      tgttgtgaa ttttcattt tagtgattt ttttgaaca 
      gaatcgata aaaccgtgc aatcgtcgg aacgttgtt 
      gattactac cctgcatcc gataagcat caagcaatt 
      taaaaaacg caacatgaa ttacattta aatatagaa 
      aagtgtctt tcaacgatg tgaaatccg agtgacttt 
      gaattacta aaaatgtgc aaatttaac atttagttg 
      ggatggtga gatttagtc tgagaagcg tcttgggcg 
      tcgcggtgc tgctcgtag ttggttttt cagaaacac 
      aaagccagt ttagttacg tctgcccca gaaaatttg 
      tggcgatcg gaagaaaat gttattact tcagcaagg 
      acgaaacta tttggcacg acgcccact ttaaatgta 
      gaaacctca acagtagtt tggccacaa ttaagggac 
      cccagcaag aactgagcg tgagaagat atctaaacc 
      gacgaaatg acgggaggt ttgagaggt ggttgggag 
      gacgctaca atgagaaac agtccaggt gggtttggg 
      gtgaaaatg gaaaacctc tggttttcc acggatttt 
      ctaggatga atgctaatg acaggaagt gggcatggc 
      attgcatta ttgtggatc ccactcgac aaaacaaat 
      ggtcggcaa ggagttgtg ttgagagga agcgctaca 
      tctgccaaa gggccgtta taggtaaaa ggaatatta 
      tcaggaggc agtgtactt cagcggcga ataacacca 
      gcagccagg gttgtttga cgcctaaag ttggatcct 
      cagctcctg catttttaa tcagaaaac ctcaaaagc 
      caactaaac cgtaccatg taaccacat tcgtctgtc 
      catctcaca tgatcctgg tgggccgaa agtgctact 
      tcttcagca tggacaaag ccagctgga gcaacgctt 
      actgggctt gcagagaca acaaaacca aactcgcag 
      tcatcacaa ccaaaatgc aggacaata ttttgagag 
      gatttctca aatggaact ttactggaa accacgaac 
      gctggcttg gtggaattt ttgattgga agcagcaaa 
      aatggaagt gggcgatga gtggaaaag cgatcagat 
      acaaaaatt ttgccaaag aagctctag tgggacaga 
      aaaataaca ctcaccatt gtatcacaa tcgaccctt 
      attataaca acttatgga actcgaaaa ataggctgg 
      aagagaaac actacattt gtcaagcta aatcgaaaa 
      gtgtggtaa aatatgata aattgtggg accataatg 
      cttcagttg tcatccaac ctgagggcc aggaggaat 
      ttattagta aaaaatcta aacaaaatt ttgctgcca 
      catctagtt cgccactag attttaata atgaagtca 
      cggataagt acgtttata attaaatat acctataca 
      attttagtg cctagaaca tcattcttg aatctccca 
      catattcaa tcaacagac aaaatgtaa atagtattt 
      ttcaatatt taataagtt agtaataca tatttgtag 
      cttta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY