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Gene information
General information
TC033006
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010897
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 1810876..1855165
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033006
    iBeetle screen iB_04931

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    egg chamber shape irregular - Number of animals showing the phenotype: 4

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (22 dpi)
    Days post injection


    Pupal morphology
    head orientation irregular - Number of animals showing the phenotype: 10

    wings not present - Number of animals showing the phenotype: 10

    pronotum shape irregular - Number of animals showing the phenotype: 10

    dorsal thoracic muscles not present - Number of animals showing the phenotype: 10

    abdomen gin-traps not present - Number of animals showing the phenotype: 10

    Adult morphology
    elytra not present - Number of animals showing the phenotype: 10


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033006 RA
      MSCSEVMYQ AYYPYLYQR AGAAPPVSA HPVPRTGPF 
      SSPFGAAHQ YDRFNVQRL QDAHSLSQP SSSGAVSAS 
      VGGLGESHP VAGHGLYLQ GSAHSSGGS VSSTGGASP 
      TSPLRPGKE EDCSLHGRS STEDPQHGI DDDDSQDAG 
      SSRAQYVSA NCVVFTHYQ GDAASVVDE HFSRALDKT 
      STHTKESSP MSARNFPPS FWDSQHYSG VSGGGPGHH 
      GASAADLYS EHYHPGDAW YQYSQHHRA VHDYHHHNM 
      AAQYGGLLL PGSRLHMGS HAPCKAMDW QHPSIDSPY 
      SSYPTMSEQ PLTNPSSFP PWVDSADAS NCHSPHRRF 
      NMAAQRLHT QCIADIEIE YEIILPPDS RNTGLDWGR 
      VIDERHVLW GRQTLFVV
    CDS sequence
    • >TC033006 RA
      atgagttgt tctgaagtt atgtaccaa gcgtattat 
      ccttacttg taccaaagg gccggagca gcgccgcca 
      gtgtcggcc catccagtg ccaagaact ggacccttt 
      agttcgccg tttggggcc gcacatcag tatgatagg 
      tttaatgta cagaggctg caagacgcc cactctctc 
      tcgcagccc agttccagt ggggcagtt agcgctagt 
      gtcggaggg ttgggggag tcacacccg gtcgcggga 
      cacggcctg tacctccag ggctctgcc cacagttcg 
      gggggctcc gtcagttcg acgggtggc gcctcaccc 
      accagcccc ctcagaccc ggcaaagaa gaggattgc 
      agtctgcac ggcaggagt agcacggaa gatcctcaa 
      cacggcatc gacgacgat gattcccaa gatgccgga 
      agttcacgt gctcaatac gtttcggcg aattgtgtc 
      gtcttcacg cactaccaa ggcgatgct gcgtctgta 
      gtcgatgaa catttttcc agggctttg gacaaaacg 
      tccacgcac acgaaagaa agttcgccg atgtccgct 
      cgcaatttc ccgccctcg ttttgggat agtcagcat 
      tacagtgga gtcagtggc ggagggcca ggtcatcac 
      ggggcgtca gctgctgat ctttactcg gagcattac 
      caccctggt gatgcttgg taccaatac agtcaacat 
      cacagagct gtgcacgat tatcatcac cacaatatg 
      gccgcccaa tatggcggc cttttactt ccgggatcc 
      aggctgcat atggggtcg catgcgcct tgtaaagcg 
      atggattgg caacatccg tctatagat tcgccgtac 
      tcttcttat cctactatg tcagagcag ccactgacc 
      aacccatcg tccttcccc ccttgggtg gattccgcc 
      gacgcctct aattgccat tctccgcat cgccgcttc 
      aatatggca gcacaacga ttacatacg caatgcata 
      gcagacatc gagatagag tacgagata atattgccg 
      ccggattcg aggaatacg gggttggat tggggtcgc 
      gtgatcgac gaacgtcac gtgttatgg gggagacaa 
      acgcttttt gtggtctaa
    mRNA sequence
    • >TC033006 RA
      tagggcgta acaccaggg tggggatag agaggcgga 
      gtcaagtaa atacagggg gtaaggaat ttttattcc 
      ggataccca cttagttcc aataccggt tctgcacga 
      ggtgtatgt cgtgatttc catccgtga cgtaacgtg 
      atttccgtg gtaaactgt caagaattg aaaatatgt 
      gtcgctaaa cactgacag gatgtaatt gcttcgagc 
      cacaatgat accctaaca aaaaaaatc gcgagtgtt 
      gttgttgca aagtgccct ttgccgacg gttttcgct 
      tttcgttgc ttttgttat tatcgtttt gtgacttga 
      ttgattttt ctgtactga gttgggagt ggtgcaata 
      atagtgttg cgtctgagg cgttttgca atgtgtata 
      tgtatataa tgagttgtt ctgaagtta tgtaccaag 
      cgtattatc cttacttgt accaaaggg ccggagcag 
      cgccgccag tgtcggccc atccagtgc caagaactg 
      gacccttta gttcgccgt ttggggccg cacatcagt 
      atgataggt ttaatgtac agaggctgc aagacgccc 
      actctctct cgcagccca gttccagtg gggcagtta 
      gcgctagtg tcggagggt tgggggagt cacacccgg 
      tcgcgggac acggcctgt acctccagg gctctgccc 
      acagttcgg ggggctccg tcagttcga cgggtggcg 
      cctcaccca ccagccccc tcagacccg gcaaagaag 
      aggattgca gtctgcacg gcaggagta gcacggaag 
      atcctcaac acggcatcg acgacgatg attcccaag 
      atgccggaa gttcacgtg ctcaatacg tttcggcga 
      attgtgtcg tcttcacgc actaccaag gcgatgctg 
      cgtctgtag tcgatgaac atttttcca gggctttgg 
      acaaaacgt ccacgcaca cgaaagaaa gttcgccga 
      tgtccgctc gcaatttcc cgccctcgt tttgggata 
      gtcagcatt acagtggag tcagtggcg gagggccag 
      gtcatcacg gggcgtcag ctgctgatc tttactcgg 
      agcattacc accctggtg atgcttggt accaataca 
      gtcaacatc acagagctg tgcacgatt atcatcacc 
      acaatatgg ccgcccaat atggcggcc ttttacttc 
      cgggatcca ggctgcata tggggtcgc atgcgcctt 
      gtaaagcga tggattggc aacatccgt ctatagatt 
      cgccgtact cttcttatc ctactatgt cagagcagc 
      cactgacca acccatcgt ccttccccc cttgggtgg 
      attccgccg acgcctcta attgccatt ctccgcatc 
      gccgcttca atatggcag cacaacgat tacatacgc 
      aatgcatag cagacatcg agatagagt acgagataa 
      tattgccgc cggattcga ggaatacgg ggttggatt 
      ggggtcgcg tgatcgacg aacgtcacg tgttatggg 
      ggagacaaa cgctttttg tggtctaat ggttggtaa 
      ttatgtagg gggtggtgc gacggacgg acgactcga 
      atggctttt taaagattg tgtttattt tcaaacaaa 
      tgcttgtag gtacgaggg tgttttttc tagatttgt 
      tgtttctgg gggaaaatg caccaacgt cattaatta 
      ttacgtaaa tgaaacgct ttaccatgt ctcgcaagg 
      aagtgcaaa taagcataa aaaataaat tgatctgtt 
      tatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY