Status
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Gene information
General information
TC010896 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 1861531..1863115
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010896
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04930

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010896 RA
      MSARRRRSG GRNRRIQPV GLQALRSDA DFEDMPLSM 
      LALLQASAR KKTPQRRRK NAPRARPSV QTTSVETIN 
      LDESVENAG IVERVNSKI DTFMETASF ISSDEEEAE 
      MIKAKTNKK PVPNQFFIS DDSDDDIQV VNVDKKTST 
      AGTSTSTAT SNESKAEAT EDDIMHLID TVLDNSNQL 
      NETAANQAK SLEEYRKET EEMLKKASS LLDEFKTGR 
      KPEPEVQKV ESPKVTVGS CPICLEALS ERPAAVTVC 
      GHIFCKECI TQTAKAMKK CPTCRKAIT VKKIHPIYF
    CDS sequence
    • >TC010896 RA
      atgagtgca cgaagaaga cgctcaggg ggaagaaac 
      cgccgtata cagcccgtc gggctgcag gctctacga 
      tcggacgca gatttcgag gacatgccc ttatctatg 
      ttagcacta ttacaagca tcggctcga aaaaaaact 
      ccccaaagg aggaggaaa aacgcacct agggctagg 
      ccctctgtt cagacgacg tctgttgaa accataaac 
      ttggatgag agtgttgaa aatgccgga atagtggaa 
      agagtgaac tcaaagatt gacacgttt atggagacg 
      gctagtttt atttcaagt gatgaggag gaggctgag 
      atgatcaag gcgaaaact aacaaaaaa ccagtgcca 
      aatcagttt tttatttca gacgactct gatgatgac 
      attcaagtt gtaaatgtg gacaagaaa acaagtact 
      gctgggaca agtactagc acggcaacg tccaacgag 
      tctaaagca gaagcgaca gaagatgat ataatgcac 
      ttgattgat acggttcta gacaactca aatcagtta 
      aatgaaaca gcggcaaat caggcgaaa agtttggaa 
      gagtatagg aaagaaacc gaagaaatg ttgaaaaaa 
      gcgtcttcg cttttagat gaattcaag actggtagg 
      aaacctgaa cccgaagtt cagaaagta gaatctccg 
      aaagtaacg gttgggtct tgtccaata tgcttagaa 
      gctctgagt gagcgccca gctgcggtc acagtttgt 
      ggccatatt ttctgtaaa gagtgcatt acccaaaca 
      gctaaggct atgaagaaa tgcccaacc tgccgtaaa 
      gcaattacc gttaaaaaa attcatcca atttatttc 
      taa
    mRNA sequence
    • >TC010896 RA
      cagctgaaa agcaactga aaagtcttt gaaataaac 
      aattttcaa attgtatta aattaataa caactgtaa 
      atgaagaac ggtaatagt gtttcttga taaaacaag 
      tgttagttt gttctataa ttcgcgagt ttgcatttt 
      gcctagttt tccttcaag taaatcaat aatatcaac 
      cgacgaatt agtttattc gtttctaac ctcaaaaat 
      gagtgcacg aagaagacg ctcaggggg aagaaaccg 
      ccgtataca gcccgtcgg gctgcaggc tctacgatc 
      ggacgcaga tttcgagga catgccctt atctatgtt 
      agcactatt acaagcatc ggctcgaaa aaaaactcc 
      ccaaaggag gaggaaaaa cgcacctag ggctaggcc 
      ctctgttca gacgacgtc tgttgaaac cataaactt 
      ggatgagag tgttgaaaa tgccggaat agtggaaag 
      agtgaactc aaagattga cacgtttat ggagacggc 
      tagttttat ttcaagtga tgaggagga ggctgagat 
      gatcaaggc gaaaactaa caaaaaacc agtgccaaa 
      tcagttttt tatttcaga cgactctga tgatgacat 
      tcaagttgt aaatgtgga caagaaaac aagtactgc 
      tgggacaag tactagcac ggcaacgtc caacgagtc 
      taaagcaga agcgacaga agatgatat aatgcactt 
      gattgatac ggttctaga caactcaaa tcagttaaa 
      tgaaacagc ggcaaatca ggcgaaaag tttggaaga 
      gtataggaa agaaaccga agaaatgtt gaaaaaagc 
      gtcttcgct tttagatga attcaagac tggtaggaa 
      acctgaacc cgaagttca gaaagtaga atctccgaa 
      agtaacggt tgggtcttg tccaatatg cttagaagc 
      tctgagtga gcgcccagc tgcggtcac agtttgtgg 
      ccatatttt ctgtaaaga gtgcattac ccaaacagc 
      taaggctat gaagaaatg cccaacctg ccgtaaagc 
      aattaccgt taaaaaaat tcatccaat ttatttcta 
      atgatttta tgtgttcaa tttttcgta cttaaaaat 
      gaattggaa tttgtgttt ttttttggt agccatctt 
      gaacccatt gtgatactg aaagtaggg gtgatcaat 
      ttttgggct acttttttt gtaccgttg tagcaagaa 
      tttatatta atttacttc aaattttgt aaaaaacat 
      aagtttttt gctgataca ttaaaattt cttgcatta 
      ttaattatc cttttatgt attctcgga ggactaggt 
      tttaaggct cgactcact atgtcatct tgctatagc 
      tacttataa accattatt ctaataagt gtatttttt 
      tattaccaa ttaaatact ttgttctgt a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY