Status
Please wait ...
Gene information
General information
TC010895 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 1879820..1886265
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010895
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04929

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages sometimes shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages sometimes orientation irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs partially distaldecreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010895 RA
      MALTNEKVQ TYGTETTVN ESTETHPGW MFWKKRRYV 
      VATLAFFGF FNVYALRVN LSIAIVAMT ENKSITLDN 
      GTIIYGPEF DWDSKIQGY ILSSFFYGY ITTQLFGGW 
      LSARIGGKR VFGGGIAIT AALTLITPW LAKVNVYLL 
      LAVRIIEGI FEGVTYPCI HAVWARWAP PLERTRLAT 
      LAFSGSYVG TVVSMPVCA YLAKALGWP SIFYFFGTL 
      GLIWFGIWW VVVAETPAE DSKITKAEL KYIKESLGH 
      SDGNRRITH PWKEILLSM PVWAIVVSH FSENWGFYT 
      LLTQLPKFM KEILNFELG KTGFMSALP YLAMSIMIQ 
      FSGHLADWL QEKGILNTT QVRKLFNCG AFIAQTVFM 
      LGAAFWLTP AGTISCLTL AVGLGGFAW SGFSVNYLD 
      VAPQHASVL MGLGNTVGT LPGILSPII SGYVVTTPT 
      AQEWQIIFY IASGVYLFG CVFYGVFAS GEVQPWAIQ 
      KEEGKGTPM KERETKGHD NKSFEPDL
    CDS sequence
    • >TC010895 RA
      atggcactc acgaatgaa aaagtgcaa acgtatggc 
      actgaaact actgtgaat gaaagcacc gaaacgcac 
      ccgggatgg atgttttgg aagaaacga agatatgtt 
      gtagcaact ttagctttc ttcggcttt ttcaacgtc 
      tacgccctt cgggtgaac ctaagcata gccatagta 
      gcaatgaca gaaaacaaa tcaatcaca ctagacaat 
      ggtacaatt atttatggg ccagaattt gactgggat 
      tcgaaaatc caaggctac atcctcagc tcgtttttt 
      tatgggtac atcacgacc cagttattt ggcggctgg 
      ttgtctgca agaatcggc gggaaacgc gttttcggg 
      ggaggaatt gccatcaca gctgcgtta actctaata 
      accccatgg ttggctaaa gtcaacgtt tacctgctg 
      ttagcggtt cgaataatc gaggggatt ttcgagggg 
      gttacgtac ccctgcatt cacgccgtt tgggcccga 
      tgggcacct cccttagag cggacaaga ctggcaact 
      ttggccttt tctgggagc tatgtaggg acggtggtt 
      tcaatgccc gtctgtgcc tatttggcc aaggcgtta 
      ggttggccc agcattttt tactttttt ggaacactt 
      ggtctcatc tggttcggt atctggtgg gtggtcgtg 
      gccgagacc ccagctgaa gattccaaa atcacgaaa 
      gcagaattg aaatatatc aaagaatcg ttgggtcat 
      tctgatggt aataggaga attacacac ccatggaag 
      gaaattctg ctttcgatg cccgtgtgg gctatagtt 
      gtgtcgcac tttagcgaa aattggggc ttttacaca 
      cttttaacc caactgccg aagtttatg aaggaaatt 
      ctcaatttc gagttgggc aaaactggg tttatgtcg 
      gcgttacct tacttggca atgtccatt atgatacaa 
      ttttcgggg catttggcc gattggttg caagagaag 
      gggattttg aatacgact caagtgaga aaactgttc 
      aactgtggg gcctttatt gcccaaact gttttcatg 
      ttgggagcg gcgttttgg ctaacacct gcaggaaca 
      atcagttgt ttgacgttg gctgttggg ttgggtggg 
      tttgcttgg tctgggttc agtgtgaat tatttggac 
      gtggcgccg caacatgcc agtgtttta atgggtttg 
      gggaacaca gtggggact ttacctggg attttgagt 
      cctattata tcaggatat gtcgtgacg actcccact 
      gcccaagag tggcaaatt attttttat atagccagt 
      ggtgtttat ctgtttgga tgcgtgttt tatggtgtg 
      tttgcgtcc ggtgaggtc cagccgtgg gctattcag 
      aaggaagaa ggcaaaggt acgccgatg aaagaacga 
      gagacgaag ggtcatgat aacaaatct tttgagcca 
      gatttataa
    mRNA sequence
    • >TC010895 RA
      gcggttttg catgttcag tgtgagtgt ggaagagtg 
      gtcttcaat atggcactc acgaatgaa aaagtgcaa 
      acgtatggc actgaaact actgtgaat gaaagcacc 
      gaaacgcac ccgggatgg atgttttgg aagaaacga 
      agatatgtt gtagcaact ttagctttc ttcggcttt 
      ttcaacgtc tacgccctt cgggtgaac ctaagcata 
      gccatagta gcaatgaca gaaaacaaa tcaatcaca 
      ctagacaat ggtacaatt atttatggg ccagaattt 
      gactgggat tcgaaaatc caaggctac atcctcagc 
      tcgtttttt tatgggtac atcacgacc cagttattt 
      ggcggctgg ttgtctgca agaatcggc gggaaacgc 
      gttttcggg ggaggaatt gccatcaca gctgcgtta 
      actctaata accccatgg ttggctaaa gtcaacgtt 
      tacctgctg ttagcggtt cgaataatc gaggggatt 
      ttcgagggg gttacgtac ccctgcatt cacgccgtt 
      tgggcccga tgggcacct cccttagag cggacaaga 
      ctggcaact ttggccttt tctgggagc tatgtaggg 
      acggtggtt tcaatgccc gtctgtgcc tatttggcc 
      aaggcgtta ggttggccc agcattttt tactttttt 
      ggaacactt ggtctcatc tggttcggt atctggtgg 
      gtggtcgtg gccgagacc ccagctgaa gattccaaa 
      atcacgaaa gcagaattg aaatatatc aaagaatcg 
      ttgggtcat tctgatggt aataggaga attacacac 
      ccatggaag gaaattctg ctttcgatg cccgtgtgg 
      gctatagtt gtgtcgcac tttagcgaa aattggggc 
      ttttacaca cttttaacc caactgccg aagtttatg 
      aaggaaatt ctcaatttc gagttgggc aaaactggg 
      tttatgtcg gcgttacct tacttggca atgtccatt 
      atgatacaa ttttcgggg catttggcc gattggttg 
      caagagaag gggattttg aatacgact caagtgaga 
      aaactgttc aactgtggg gcctttatt gcccaaact 
      gttttcatg ttgggagcg gcgttttgg ctaacacct 
      gcaggaaca atcagttgt ttgacgttg gctgttggg 
      ttgggtggg tttgcttgg tctgggttc agtgtgaat 
      tatttggac gtggcgccg caacatgcc agtgtttta 
      atgggtttg gggaacaca gtggggact ttacctggg 
      attttgagt cctattata tcaggatat gtcgtgacg 
      actcccact gcccaagag tggcaaatt attttttat 
      atagccagt ggtgtttat ctgtttgga tgcgtgttt 
      tatggtgtg tttgcgtcc ggtgaggtc cagccgtgg 
      gctattcag aaggaagaa ggcaaaggt acgccgatg 
      aaagaacga gagacgaag ggtcatgat aacaaatct 
      tttgagcca gatttataa ttagtgcaa ttttagtga 
      caatttagt aattaatgt gatatttta ggctagact 
      tttgtagag aatactctc cttgcaatc tttttaacc 
      aaataatgt aacagttta gagaattga ataaaaaaa 
      attgaaaca a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY