Status
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Gene information
General information
TC033004
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010893
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 1937431..1988541
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033004
    iBeetle screen iB_04928

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033004 RA
      MENLTQLFQ NFPPCCAPD ESGTLKRGC GRRWTSLKQ 
      HPRSCTEPL TETGNSSGL STPSTTNTS PLPVAAGEG 
      TRKSNWQVI EHFSSKDKG SLSSSLIAV GSVSRTPPA 
      KENGSAPNS SPDLENHEV EAELLVSSI PKQSTGFFD 
      KLLRLIKKL FSTHQFQNE QVEMLYQRY FLKMNQSNM 
      TNLISLLLL LCVGFLLFA AIDIVMVHH SETDTLQEH 
      SFRLDKSLV LICTSGCCI AIYGVLLAV LSKPAMNEV 
      YLIIISYFI LVTFLTLEI CISFFSETE RPLLGPTFS 
      LATTYLTYA LMPIRLKDA LLAGTVLAL TQVILLVYV 
      GQLYAIADI TSCFVILLC GNVAGVCTH YPREVAQRK 
      AFLETRQCI EARLKIQRE NQQQERLLL SVLPRHVAM 
      EMKADIAGQ PKEAQFHKI YIQRHENVS ILFADICGF 
      TTLSDQCTA EELVRILNE LFARFDRLA AEHHCLRIK 
      LLGDCYYCV SGLPEARPD HAHCTVEMG LDMIDAIAL 
      VREVMSVNV NMRVGIHTG RVHCGVLGL RKWQFDVWS 
      NDVTLANHM ESGGIPGRV HITKETLKC LGNDYVVEP 
      GNGGDRNSY LKDHNIDTY LIVAPSYRG GSTPYQNHS 
      MNGNISKEM RVMGHGSQH GKHSSKLGF SEPPEREKN 
      PEDEVNEYL MRAIDARSI DRLRTEHCR TLLLHFRDP 
      VKEAKYILE KDRMLTLYF FCSTFCFTA IFLVQIVVM 
      RRNDTAYLC SIPIMFLLI LIPVIVYME NVNLENVHL 
      KIFAVSKYI HKNRNIAQI FSFIMAVCI YGLVMLHTL 
      SLPEEIQCL NSTIVDSNC TNFTVYSLN NDNKDCKPY 
      FLIDYLLLL VFISMIACA VYQVLISLF KTIILLGGM 
      AGFIAAYIT YTKEVHAMH KFFCIPKQS ETTLTQQYV 
      NIVILVGFV IALILHGQQ TEATYRLDF VWKLQATEE 
      KEDMEHLEA YNRKLLANI LPVHVAEHF LNSDKNNDE 
      LYHEQCDFV CIMFASIPN FSEFYVELE GNNEGVECL 
      RLLNEIIAD FDELLSEDQ FKYIEKIKS TGATYMAAS 
      GLTQNTCDM QGYRHVTAM ADYALRIQE QLAEVNEHS 
      FNHFRIRIG INVGPVVAG VIGARKPQY DIWGNAVNV 
      ASRMDSTGV LDKIQVTKE VYQILSSRG YPLTCRGTI 
      QVKGKGTME TYFLEGRCK KPTQSETNI TVNPLANIN 
      SDCSKTFLP HQTTVLDTI SEKI
    CDS sequence
    • >TC033004 RA
      atggaaaac cttacgcaa cttttccaa aatttccct 
      ccctgctgt gcacctgac gaaagtggt acattaaaa 
      cggggctgc ggtcgccgt tggactagc ctgaaacaa 
      caccctcga tcatgtact gaacccctc acagaaacc 
      ggaaacagc agcggcctt tcaacccct tcgaccacc 
      aacacaagc cctcttccg gtggccgcg ggcgagggt 
      accaggaag agtaattgg caggtgatt gagcacttt 
      agttcaaag gataaagga agtctttca tcaagtctg 
      atagcggtt ggcagtgtg tctcggacg cctccagcg 
      aaagaaaac ggttcagcc ccgaactca agtccagat 
      ttagaaaat catgaagtc gaagcagaa ttacttgtg 
      tcttcgatc ccgaagcaa tcgacaggt tttttcgac 
      aaacttcta agactcatt aagaaatta tttagtact 
      catcagttt caaaatgaa caggttgaa atgctatac 
      cagcgatat ttcctaaaa atgaatcaa agcaatatg 
      acgaatttg atatctctc ctactcttg ttatgcgtc 
      ggctttctc ctcttcgct gctattgat atcgtaatg 
      gtacaccac tcagaaaca gatacactt caagaacac 
      tcattccga ttagacaaa tcacttgta ctcatttgc 
      acgtccggc tgttgtatt gcaatttat ggagttctt 
      ttagcggtt ctctccaag cctgcgatg aacgaggtg 
      tacctgata attatttcc tactttatt ctagtcaca 
      tttcttacg ttagagata tgtatttcg tttttttcc 
      gagacggag cggccgtta ttgggcccc acgttttcg 
      ctagctacc acatactta acatacgct ttgatgcca 
      atccgtctc aaagacgcc cttctagcc ggcaccgtt 
      ttggctctg acccaagtt attctactt gtttatgtg 
      ggacaattg tacgccatc gcagatatc accagctgc 
      tttgtgatt ctgctgtgc ggaaacgtc gccggcgtt 
      tgcacccat tatcctcga gaagtggcc caacgtaaa 
      gctttttta gaaacgaga caatgcata gaagcccgc 
      ctgaaaatc cagcgagag aatcagcaa caggaacgt 
      cttctcctg tcagtatta ccgaggcac gtcgctatg 
      gagatgaag gcggatatt gccggacaa ccgaaagaa 
      gcccaattt cataaaata tacatccaa aggcatgag 
      aatgtcagc attctcttc gctgacatt tgcggtttc 
      acgacgctc tccgaccaa tgcactgct gaagagttg 
      gtccgaatc ctgaacgag ctctttgcc aggtttgac 
      cggctggcg gccgaacat cactgtttg agaattaaa 
      ctgctggga gactgttac tattgcgtt tccggttta 
      ccggaagcc aggccggat cacgcccat tgcacggtt 
      gaaatgggg ctggatatg attgacgcc atagccttg 
      gtgcgtgaa gtgatgtca gtcaacgtt aatatgcgg 
      gttggaatc cataccgga cgcgtccat tgcggagta 
      ttaggactt cgcaaatgg caatttgac gtgtggtcg 
      aacgacgtc actttagcc aatcacatg gaaagtggc 
      ggcattcca ggacgcgtc cacattacc aaagaaaca 
      ctgaaatgt ttgggcaac gattacgtc gtcgagcca 
      ggcaacggc ggtgaccgc aattcttat ctcaaagat 
      cacaacatc gacacgtac ttaattgta gcgccgtcg 
      tatagaggg ggctcgaca ccttaccag aatcattca 
      atgaacgga aacatctcg aaggagatg agggtgatg 
      ggccacgga tcacaacat ggaaagcat tcgtctaaa 
      ctgggtttc agcgaacct cctgaaagg gagaaaaac 
      cctgaagat gaagtgaac gagtatttg atgagagcc 
      attgatgct cgcagcata gacagatta aggacggaa 
      cattgccgg accttactt ctccatttt cgtgatcca 
      gtcaaggag gccaaatac attttggag aaggacagg 
      atgctcact ttgtatttt ttctgctcc acgttttgt 
      ttcaccgcg attttttta gtccagatt gttgtgatg 
      cgaaggaac gacacagca tatttgtgc tcaattcca 
      atcatgttt cttctgatt ttaattccg gtaatagta 
      tacatggaa aacgtcaat ttggagaat gtccacttg 
      aaaattttt gcagtgtca aaatacata cacaaaaat 
      aggaacatt gctcaaata ttttctttc ataatggct 
      gtttgtata tatggttta gtaatgtta catacgctc 
      tcccttcct gaggaaatt cagtgtctg aattcaacc 
      attgtcgat agtaactgt acgaacttt actgtttat 
      tcgctgaat aacgacaac aaagactgc aagccgtac 
      ttcttgata gattatctg ttacttctt gtttttatc 
      tcgatgatt gcgtgcgcc gtttatcaa gtccttatt 
      tcacttttc aagacaatc attctcttg ggtggaatg 
      gctggattt atcgctgct tatattaca tacaccaaa 
      gaagtgcac gctatgcac aagtttttt tgcatacct 
      aagcaaagt gaaacgact ttgacacaa caatacgtt 
      aacattgtg atattagtc ggctttgta atagctcta 
      atattacac ggccaacaa acagaagcc acttataga 
      ttggatttc gtttggaaa ttacaagcc acagaggag 
      aaagaagac atggaacac ttagaagcg tacaaccgt 
      aaactgctc gctaatatc ttgccggtt catgtcgct 
      gagcatttc ctcaacagt gataaaaat aacgacgaa 
      ctctaccac gaacaatgc gattttgtc tgcatcatg 
      ttcgcttcg attccaaat ttctccgaa ttttacgtc 
      gaacttgaa ggcaacaac gaaggagtt gaatgtctg 
      cggctcttg aatgaaatt atcgcagac ttcgacgag 
      ctgctctcc gaagaccag ttcaagtac atcgagaag 
      atcaaatca actggggcc acatacatg gcggcttct 
      ggattgact cagaacacc tgcgacatg cagggctat 
      cggcacgtg accgcgatg gccgactac gccctccgc 
      atccaggaa caactggca gaagtcaac gagcattcc 
      ttcaatcat ttccggatc cggatcggg attaatgtg 
      ggccccgtg gtggctggc gtcattggg gcgcggaag 
      ccccagtat gatatatgg ggcaacgcc gtcaacgtg 
      gcgagtcga atggatagc accggagtt ctagataag 
      attcaagtg acgaaggaa gtgtaccaa atcttgagc 
      tccagagga tacccgtta acgtgcaga ggaacgata 
      caggtgaag ggcaaaggg acgatggag acgtatttc 
      ctcgaagga cggtgcaag aagccgacg caatcagag 
      acgaatatt actgtgaat cctttagct aatattaac 
      tcagattgt agcaaaact ttcttacct catcaaaca 
      actgtgtta gatactatc tctgagaag atctaa
    mRNA sequence
    • >TC033004 RA
      aagctactt aagaaaaaa tggaaaacc ttacgcaac 
      ttttccaaa atttccctc cctgctgtg cacctgacg 
      aaagtggta cattaaaac ggggctgcg gtcgccgtt 
      ggactagcc tgaaacaac accctcgat catgtactg 
      aacccctca cagaaaccg gaaacagca gcggccttt 
      caacccctt cgaccacca acacaagcc ctcttccgg 
      tggccgcgg gcgagggta ccaggaaga gtaattggc 
      aggtgattg agcacttta gttcaaagg ataaaggaa 
      gtctttcat caagtctga tagcggttg gcagtgtgt 
      ctcggacgc ctccagcga aagaaaacg gttcagccc 
      cgaactcaa gtccagatt tagaaaatc atgaagtcg 
      aagcagaat tacttgtgt cttcgatcc cgaagcaat 
      cgacaggtt ttttcgaca aacttctaa gactcatta 
      agaaattat ttagtactc atcagtttc aaaatgaac 
      aggttgaaa tgctatacc agcgatatt tcctaaaaa 
      tgaatcaaa gcaatatga cgaatttga tatctctcc 
      tactcttgt tatgcgtcg gctttctcc tcttcgctg 
      ctattgata tcgtaatgg tacaccact cagaaacag 
      atacacttc aagaacact cattccgat tagacaaat 
      cacttgtac tcatttgca cgtccggct gttgtattg 
      caatttatg gagttcttt tagcggttc tctccaagc 
      ctgcgatga acgaggtgt acctgataa ttatttcct 
      actttattc tagtcacat ttcttacgt tagagatat 
      gtatttcgt ttttttccg agacggagc ggccgttat 
      tgggcccca cgttttcgc tagctacca catacttaa 
      catacgctt tgatgccaa tccgtctca aagacgccc 
      ttctagccg gcaccgttt tggctctga cccaagtta 
      ttctacttg tttatgtgg gacaattgt acgccatcg 
      cagatatca ccagctgct ttgtgattc tgctgtgcg 
      gaaacgtcg ccggcgttt gcacccatt atcctcgag 
      aagtggccc aacgtaaag cttttttag aaacgagac 
      aatgcatag aagcccgcc tgaaaatcc agcgagaga 
      atcagcaac aggaacgtc ttctcctgt cagtattac 
      cgaggcacg tcgctatgg agatgaagg cggatattg 
      ccggacaac cgaaagaag cccaatttc ataaaatat 
      acatccaaa ggcatgaga atgtcagca ttctcttcg 
      ctgacattt gcggtttca cgacgctct ccgaccaat 
      gcactgctg aagagttgg tccgaatcc tgaacgagc 
      tctttgcca ggtttgacc ggctggcgg ccgaacatc 
      actgtttga gaattaaac tgctgggag actgttact 
      attgcgttt ccggtttac cggaagcca ggccggatc 
      acgcccatt gcacggttg aaatggggc tggatatga 
      ttgacgcca tagccttgg tgcgtgaag tgatgtcag 
      tcaacgtta atatgcggg ttggaatcc ataccggac 
      gcgtccatt gcggagtat taggacttc gcaaatggc 
      aatttgacg tgtggtcga acgacgtca ctttagcca 
      atcacatgg aaagtggcg gcattccag gacgcgtcc 
      acattacca aagaaacac tgaaatgtt tgggcaacg 
      attacgtcg tcgagccag gcaacggcg gtgaccgca 
      attcttatc tcaaagatc acaacatcg acacgtact 
      taattgtag cgccgtcgt atagagggg gctcgacac 
      cttaccaga atcattcaa tgaacggaa acatctcga 
      aggagatga gggtgatgg gccacggat cacaacatg 
      gaaagcatt cgtctaaac tgggtttca gcgaacctc 
      ctgaaaggg agaaaaacc ctgaagatg aagtgaacg 
      agtatttga tgagagcca ttgatgctc gcagcatag 
      acagattaa ggacggaac attgccgga ccttacttc 
      tccattttc gtgatccag tcaaggagg ccaaataca 
      ttttggaga aggacagga tgctcactt tgtattttt 
      tctgctcca cgttttgtt tcaccgcga tttttttag 
      tccagattg ttgtgatgc gaaggaacg acacagcat 
      atttgtgct caattccaa tcatgtttc ttctgattt 
      taattccgg taatagtat acatggaaa acgtcaatt 
      tggagaatg tccacttga aaatttttg cagtgtcaa 
      aatacatac acaaaaata ggaacattg ctcaaatat 
      tttctttca taatggctg tttgtatat atggtttag 
      taatgttac atacgctct cccttcctg aggaaattc 
      agtgtctga attcaacca ttgtcgata gtaactgta 
      cgaacttta ctgtttatt cgctgaata acgacaaca 
      aagactgca agccgtact tcttgatag attatctgt 
      tacttcttg tttttatct cgatgattg cgtgcgccg 
      tttatcaag tccttattt cacttttca agacaatca 
      ttctcttgg gtggaatgg ctggattta tcgctgctt 
      atattacat acaccaaag aagtgcacg ctatgcaca 
      agttttttt gcataccta agcaaagtg aaacgactt 
      tgacacaac aatacgtta acattgtga tattagtcg 
      gctttgtaa tagctctaa tattacacg gccaacaaa 
      cagaagcca cttatagat tggatttcg tttggaaat 
      tacaagcca cagaggaga aagaagaca tggaacact 
      tagaagcgt acaaccgta aactgctcg ctaatatct 
      tgccggttc atgtcgctg agcatttcc tcaacagtg 
      ataaaaata acgacgaac tctaccacg aacaatgcg 
      attttgtct gcatcatgt tcgcttcga ttccaaatt 
      tctccgaat tttacgtcg aacttgaag gcaacaacg 
      aaggagttg aatgtctgc ggctcttga atgaaatta 
      tcgcagact tcgacgagc tgctctccg aagaccagt 
      tcaagtaca tcgagaaga tcaaatcaa ctggggcca 
      catacatgg cggcttctg gattgactc agaacacct 
      gcgacatgc agggctatc ggcacgtga ccgcgatgg 
      ccgactacg ccctccgca tccaggaac aactggcag 
      aagtcaacg agcattcct tcaatcatt tccggatcc 
      ggatcggga ttaatgtgg gccccgtgg tggctggcg 
      tcattgggg cgcggaagc cccagtatg atatatggg 
      gcaacgccg tcaacgtgg cgagtcgaa tggatagca 
      ccggagttc tagataaga ttcaagtga cgaaggaag 
      tgtaccaaa tcttgagct ccagaggat acccgttaa 
      cgtgcagag gaacgatac aggtgaagg gcaaaggga 
      cgatggaga cgtatttcc tcgaaggac ggtgcaaga 
      agccgacgc aatcagaga cgaatatta ctgtgaatc 
      ctttagcta atattaact cagattgta gcaaaactt 
      tcttacctc atcaaacaa ctgtgttag atactatct 
      ctgagaaga tctaagctg atcatttat ttattttta 
      tattaggat gcaaggaac gaccgcttt tactcacag 
      atttgttga tattgtaca aaatgacaa taatttaat 
      aagaggaac aaaaaggcg agggttgtg agtaaggac 
      gaatcggcg agtcgcgaa gaatactct gtaaatact 
      ggtagtttt gaacttatt tttatgtat ataattttt 
      ttaataaaa cacaaaata tgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY