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Gene information
General information
TC010876 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 5036079..5041664
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010876
    iBeetle screen iB_04926

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010876 RA
      MSTPVKKVP IHLQSAQNR LLIKHGKVV NEDDITEDD 
      IYIEDGVIK QLGRNLIIP GGTRVIDAR GKYVLPGGI 
      DPHTHFEME FMGAKSVDD FYQGTKAAV AGGTTMIID 
      FVMPKKGES ILDAFYEYR QKGDDKVCC DYGLHVALT 
      HWNEQVRRE MEVLCKEHG VNSFKVFMA YGFMLNDSE 
      LYSVFETCQ KLGAVAQVH AENGAIIAK NVERLLAKG 
      ITGPEGHEM SRPEEVEAE AVNRACVIA KQVNAPIYI 
      LNVTTEESG NIIRTARSK GQTVFGEVR AGSIGLIGD 
      PRNANMITA PAIRSRPET PFQLLGLLA SDVLQVTGS 
      DNCTFNKSQ KEMGKNDFS KIPNGVNGV EDRMSVIWE 
      KGVQSGMID PCRFVAITS TNAAKIFNI YPRKGCIAV 
      GSDADLVIW DPTKTRTIS ASTHHQAVD FNIFEGMVC 
      HGVPEFVIV NGRVCVDDG QLRVVQGHG HFVETPVYA 
      PFVYDPEKI ADLKPVRNG THDDNLSEH VHKVTIEDK 
      ACPTPTLPD SAVSTPSVR GPRPEGQRN IQDSTFSIS 
      EDLDVDRKS CIRVRNPPG GKSSGFW
    • >TC010876 RB
      MSTPVKKVP IHLQSAQNR LLIKHGKVV NEDDITEDD 
      IYIEDGVIK QLGRNLIIP GGTRVIDAR GKYVLPGGI 
      DPHTHFEME FMGAKSVDD FYQGTKAAV AGGTTMIID 
      FVMPKKGES ILDAFYEYR QKGDDKVCC DYGLHVALT 
      HWNEQVRRE MEVLCKEHG VNSFKVFMA YGFMLNDSE 
      LYSVFETCQ KLGAVAQVH AENGAIIAK NVERLLAKG 
      ITGPEGHEM SRPEEVEAE AVNRACVIA KQVII
    • >TC010876 RC
      MITAPAIRS RPETPFQLL GLLASDVLQ VTGSDNCTF 
      NKSQKEMGK NDFSKIPNG VNGVEDRMS VIWEKGVQS 
      GMIDPCRFV AITSTNAAK IFNIYPRKG CIAVGSDAD 
      LVIWDPTKT RTISASTHH QAVDFNIFE GMVCHGVPE 
      FVIVNGRVC VDDGQLRVV QGHGHFVET PVYAPFVYD 
      PEKIADLKP VRNGTHDDN LSEHVHKVT IEDKACPTP 
      TLPDSAVST PSVRGPRPE GQRNIQDST FSISEDLDV 
      DRKSCIRVR NPPGGKSSG FW
    CDS sequence
    • >TC010876 RA
      atgtctact ccagtgaaa aaagttcca attcattta 
      cagagtgcc cagaaccgt ttacttatc aagcatggg 
      aaagtggta aacgaggac gacatcacc gaggatgat 
      atctacatc gaggacggg gtcataaag cagctgggg 
      cgcaatctg atcatccct gggggcacc cgcgtgatt 
      gacgctcgc ggcaagtat gtgctgccc ggcggcatc 
      gacccccac acgcacttc gagatggag ttcatgggg 
      gcgaaatca gtcgacgac ttctaccaa ggcaccaaa 
      gcggcggtg gctggcggc accaccatg ataattgac 
      ttcgtcatg ccaaagaag ggggagtca atcctggac 
      gcgttttac gaataccga caaaagggt gacgacaaa 
      gtgtgctgc gattacgga ctccacgtc gcgctaact 
      cactggaat gagcaagtc aggcgcgag atggaagtc 
      ctgtgcaag gagcacgga gttaattca tttaaagtt 
      tttatggct tatggtttt atgcttaat gattcggaa 
      ttgtatagc gtttttgaa acgtgccag aaattggga 
      gccgtggcg caagtgcat gccgagaat ggcgcgatt 
      attgcgaaa aatgtcgaa cggcttttg gccaagggc 
      atcacaggg cccgaggga cacgagatg tccaggccg 
      gaggaggtc gaggctgag gccgtcaac agggcttgt 
      gtcattgcc aaacaggtg aacgcaccc atttatatc 
      cttaatgtt accacggaa gaatcagga aatattata 
      cgaactgcg cgttctaaa ggacaaacc gtttttgga 
      gaagtacgt gccggtagt attggtctt ataggagat 
      ccacgtaat gccaacatg ataacagca ccagctatt 
      cgctctaga cctgaaacg cccttccag cttttgggg 
      ttgttggca tcggatgtc ctccaagtc accggtagt 
      gacaattgc actttcaac aagtctcag aaagagatg 
      ggcaaaaac gacttctcc aaaatcccg aacggcgta 
      aacggggtg gaggaccgc atgtcggtc atttgggag 
      aaaggggtg cagtcgggg atgatcgac ccttgtcgt 
      tttgtcgca ataactagc acaaacgca gctaaaatc 
      ttcaacatc tacccgaga aagggctgc attgcggtc 
      ggatcagac gcagacctt gtcatttgg gacccgacc 
      aaaactcga acaatttcg gcctccacg caccaccaa 
      gccgtcgat ttcaacata ttcgaaggg atggtgtgc 
      catggggtc cccgagttc gtcattgtg aacgggcgg 
      gtgtgcgtt gacgacggc cagctgcgg gtcgtccaa 
      ggccacggc cacttcgtg gagacgccc gtctacgcc 
      ccgttcgtt tacgatccc gagaaaatc gccgatttg 
      aagccggtc cgcaatggg acgcacgat gacaatttg 
      tccgaacac gttcacaag gtcacaatc gaggacaaa 
      gcctgtcct acaccaaca ctgccagac tcagctgtg 
      agcacgccc tcggtcagg gggccgcga ccggaaggt 
      caaaggaac atccaagat tcgacattt tctatcagc 
      gaggatctt gatgtggac agaaagtct tgcatccgt 
      gtgaggaac ccaccaggt ggcaaatct tccgggttt 
      tggtaa
    • >TC010876 RB
      atgtctact ccagtgaaa aaagttcca attcattta 
      cagagtgcc cagaaccgt ttacttatc aagcatggg 
      aaagtggta aacgaggac gacatcacc gaggatgat 
      atctacatc gaggacggg gtcataaag cagctgggg 
      cgcaatctg atcatccct gggggcacc cgcgtgatt 
      gacgctcgc ggcaagtat gtgctgccc ggcggcatc 
      gacccccac acgcacttc gagatggag ttcatgggg 
      gcgaaatca gtcgacgac ttctaccaa ggcaccaaa 
      gcggcggtg gctggcggc accaccatg ataattgac 
      ttcgtcatg ccaaagaag ggggagtca atcctggac 
      gcgttttac gaataccga caaaagggt gacgacaaa 
      gtgtgctgc gattacgga ctccacgtc gcgctaact 
      cactggaat gagcaagtc aggcgcgag atggaagtc 
      ctgtgcaag gagcacgga gttaattca tttaaagtt 
      tttatggct tatggtttt atgcttaat gattcggaa 
      ttgtatagc gtttttgaa acgtgccag aaattggga 
      gccgtggcg caagtgcat gccgagaat ggcgcgatt 
      attgcgaaa aatgtcgaa cggcttttg gccaagggc 
      atcacaggg cccgaggga cacgagatg tccaggccg 
      gaggaggtc gaggctgag gccgtcaac agggcttgt 
      gtcattgcc aaacaggtg attatataa
    • >TC010876 RC
      atgataaca gcaccagct attcgctct agacctgaa 
      acgcccttc cagcttttg gggttgttg gcatcggat 
      gtcctccaa gtcaccggt agtgacaat tgcactttc 
      aacaagtct cagaaagag atgggcaaa aacgacttc 
      tccaaaatc ccgaacggc gtaaacggg gtggaggac 
      cgcatgtcg gtcatttgg gagaaaggg gtgcagtcg 
      gggatgatc gacccttgt cgttttgtc gcaataact 
      agcacaaac gcagctaaa atcttcaac atctacccg 
      agaaagggc tgcattgcg gtcggatca gacgcagac 
      cttgtcatt tgggacccg accaaaact cgaacaatt 
      tcggcctcc acgcaccac caagccgtc gatttcaac 
      atattcgaa gggatggtg tgccatggg gtccccgag 
      ttcgtcatt gtgaacggg cgggtgtgc gttgacgac 
      ggccagctg cgggtcgtc caaggccac ggccacttc 
      gtggagacg cccgtctac gccccgttc gtttacgat 
      cccgagaaa atcgccgat ttgaagccg gtccgcaat 
      gggacgcac gatgacaat ttgtccgaa cacgttcac 
      aaggtcaca atcgaggac aaagcctgt cctacacca 
      acactgcca gactcagct gtgagcacg ccctcggtc 
      agggggccg cgaccggaa ggtcaaagg aacatccaa 
      gattcgaca ttttctatc agcgaggat cttgatgtg 
      gacagaaag tcttgcatc cgtgtgagg aacccacca 
      ggtggcaaa tcttccggg ttttggtaa
    mRNA sequence
    • >TC010876 RA
      cagtgccaa cattataaa tattaccag tgccgggca 
      aggtcgttc tagtctagc tataggttg ttggtgtta 
      aatttgtcg ttcgtttca gtttaattt gattgataa 
      aaaacgagg aaatcgtca ttattgaat ccactgaaa 
      ccactgagg taaaccgca atgtctact ccagtgaaa 
      aaagttcca attcattta cagagtgcc cagaaccgt 
      ttacttatc aagcatggg aaagtggta aacgaggac 
      gacatcacc gaggatgat atctacatc gaggacggg 
      gtcataaag cagctgggg cgcaatctg atcatccct 
      gggggcacc cgcgtgatt gacgctcgc ggcaagtat 
      gtgctgccc ggcggcatc gacccccac acgcacttc 
      gagatggag ttcatgggg gcgaaatca gtcgacgac 
      ttctaccaa ggcaccaaa gcggcggtg gctggcggc 
      accaccatg ataattgac ttcgtcatg ccaaagaag 
      ggggagtca atcctggac gcgttttac gaataccga 
      caaaagggt gacgacaaa gtgtgctgc gattacgga 
      ctccacgtc gcgctaact cactggaat gagcaagtc 
      aggcgcgag atggaagtc ctgtgcaag gagcacgga 
      gttaattca tttaaagtt tttatggct tatggtttt 
      atgcttaat gattcggaa ttgtatagc gtttttgaa 
      acgtgccag aaattggga gccgtggcg caagtgcat 
      gccgagaat ggcgcgatt attgcgaaa aatgtcgaa 
      cggcttttg gccaagggc atcacaggg cccgaggga 
      cacgagatg tccaggccg gaggaggtc gaggctgag 
      gccgtcaac agggcttgt gtcattgcc aaacaggtg 
      aacgcaccc atttatatc cttaatgtt accacggaa 
      gaatcagga aatattata cgaactgcg cgttctaaa 
      ggacaaacc gtttttgga gaagtacgt gccggtagt 
      attggtctt ataggagat ccacgtaat gccaacatg 
      ataacagca ccagctatt cgctctaga cctgaaacg 
      cccttccag cttttgggg ttgttggca tcggatgtc 
      ctccaagtc accggtagt gacaattgc actttcaac 
      aagtctcag aaagagatg ggcaaaaac gacttctcc 
      aaaatcccg aacggcgta aacggggtg gaggaccgc 
      atgtcggtc atttgggag aaaggggtg cagtcgggg 
      atgatcgac ccttgtcgt tttgtcgca ataactagc 
      acaaacgca gctaaaatc ttcaacatc tacccgaga 
      aagggctgc attgcggtc ggatcagac gcagacctt 
      gtcatttgg gacccgacc aaaactcga acaatttcg 
      gcctccacg caccaccaa gccgtcgat ttcaacata 
      ttcgaaggg atggtgtgc catggggtc cccgagttc 
      gtcattgtg aacgggcgg gtgtgcgtt gacgacggc 
      cagctgcgg gtcgtccaa ggccacggc cacttcgtg 
      gagacgccc gtctacgcc ccgttcgtt tacgatccc 
      gagaaaatc gccgatttg aagccggtc cgcaatggg 
      acgcacgat gacaatttg tccgaacac gttcacaag 
      gtcacaatc gaggacaaa gcctgtcct acaccaaca 
      ctgccagac tcagctgtg agcacgccc tcggtcagg 
      gggccgcga ccggaaggt caaaggaac atccaagat 
      tcgacattt tctatcagc gaggatctt gatgtggac 
      agaaagtct tgcatccgt gtgaggaac ccaccaggt 
      ggcaaatct tccgggttt tggtaatca caagaagac 
      ggcgcctca atagttatg caaattaca aaaaagtac 
      caaagcaaa ataatcttc caatcattt cattagaat 
      agttcgtat ttctattta ttacatcat ttacacatt 
      tttctgtct cttatttgt tcaaaaatg ttttatgtt 
      atatcgtag attttgtta ctgtttttt tacattaga 
      atgtattgg gagagttta tgcgtatat gaatatcaa 
      aatcaatgt tgtattgtg cagtaaatg aaaaatact 
      ggctattgc tgtacgtta aaaatttat cgagacatt 
      tgttaattt tttatttat aaaacgcga aagttaatt 
      gcatgaaag tagcacaga tgtatctgt tttaagttg 
      cactgtctc tgacaactg ttggcttgc agcctgaca 
      aattttcgt taatactaa tttgtttaa ttttattaa 
      actactcag ataatctta tattgaaaa tgaccatca 
      ataataaat tggtcttgt gattttaag tatctcaat 
      accaaatat cactagttt cgatcattt acgttccat 
      ggactttac atctaaagg tcattctgt tctatataa 
      gattatgag tggttataa gtttactaa taaacatta 
      ataacaaaa
    • >TC010876 RB
      cagtgccaa cattataaa tattaccag tgccgggca 
      aggtcgttc tagtctagc tataggttg ttggtgtta 
      aatttgtcg ttcgtttca gtttaattt gattgataa 
      aaaacgagg aaatcgtca ttattgaat ccactgaaa 
      ccactgagg taaaccgca atgtctact ccagtgaaa 
      aaagttcca attcattta cagagtgcc cagaaccgt 
      ttacttatc aagcatggg aaagtggta aacgaggac 
      gacatcacc gaggatgat atctacatc gaggacggg 
      gtcataaag cagctgggg cgcaatctg atcatccct 
      gggggcacc cgcgtgatt gacgctcgc ggcaagtat 
      gtgctgccc ggcggcatc gacccccac acgcacttc 
      gagatggag ttcatgggg gcgaaatca gtcgacgac 
      ttctaccaa ggcaccaaa gcggcggtg gctggcggc 
      accaccatg ataattgac ttcgtcatg ccaaagaag 
      ggggagtca atcctggac gcgttttac gaataccga 
      caaaagggt gacgacaaa gtgtgctgc gattacgga 
      ctccacgtc gcgctaact cactggaat gagcaagtc 
      aggcgcgag atggaagtc ctgtgcaag gagcacgga 
      gttaattca tttaaagtt tttatggct tatggtttt 
      atgcttaat gattcggaa ttgtatagc gtttttgaa 
      acgtgccag aaattggga gccgtggcg caagtgcat 
      gccgagaat ggcgcgatt attgcgaaa aatgtcgaa 
      cggcttttg gccaagggc atcacaggg cccgaggga 
      cacgagatg tccaggccg gaggaggtc gaggctgag 
      gccgtcaac agggcttgt gtcattgcc aaacaggtg 
      attatataa ccgtttccg ggaatttcc accaaataa 
      cacgcactt atgtaagat tcgtaaaca attaaacca 
      tttatctgg cgcaagaac gccaacgat tatttatcg 
      ggcaacgca atctctaca aacaaatca accgaactg 
      aaaataaac aagcacttt gcagaatta ccctggcgc 
      gtaaaattt ttgaaaata gttgtactt cgttgcgac 
      ttttactgc cctttttac acaatttta agaagcact 
      tcaaacgca acagtccgt caatcgggc gcaacgaaa 
      agcaccatg gaaacatat gctctgttt aaaatttat 
      gaaacatgt cacttcata ctgactatt tcaattcgg 
      aaatagtgt ctttctttt atacctaat ttacccgga 
      tataaacca ctta
    • >TC010876 RC
      ttgattttt tacgacttc gtcactgtt aacgttgtt 
      ttttttgag ctatctacc tgaaaaata tacagggag 
      gtgcatttt taaggtcca atcgcctaa accaactca 
      tttcgaagt attcgtaac tacgttatt ttaaaaggt 
      gcacaatta aaataaagc cacaattat ttgtttaag 
      cttcattat ctgcataga tgttgcttg taattcaca 
      atacaattg tttgtggct gaaaaaatc caactaaat 
      ttatacagc atgctccaa agatactaa aaagttcaa 
      taacttgtt aaaatattg cgggatata tacaggttc 
      atacaggtt ttaaaaata ttaaaatat tacaaccac 
      cgagttttt ttttgctta aaatatttt tgcatgttt 
      cgtttccag attaacaat gttctagaa tatttgacg 
      caaacaaaa tgtacaaaa taaatattt ctagttgaa 
      taaaaaccg atgaagaat gactgtttt ctttttatt 
      tttaggcga gcgtgtgta tttggtgga aattttgca 
      aatctttta gacaaaatt gtgaattct tttgagttt 
      gttggcact gtagaagct gtaaacttt atttctgat 
      cttagtttt cttgagatg tttagtcgt ggtggtatt 
      tataggtag attgtccgt tgtatatag tgcatgtga 
      tgagcaaat ctgcaggat tagctttaa agcaagcat 
      gtgaacgag gtacccgag tgtatggcg aaactttag 
      tagcagcac tagcttcag atggttccc attacaagc 
      atgagtgct tccagcact cggctggcc acattttaa 
      gccctccct taagaccgg atcccacaa caccgcaat 
      ttttaatga atctgttag cacagtaag tttgtgttt 
      ttcgtttgc tcttttagt tcttcattt ccactaatt 
      gaggttcat tcgtttcac gattaaccc ttgaatgtt 
      gcattggct ttctgttta gcactttct gtaccgata 
      gtgtagcag tgagaaaac agttttgag tgcaggtga 
      acgcaccca tttatatcc ttaatgtta ccacggaag 
      aatcaggaa atattatac gaactgcgc gttctaaag 
      gacaaaccg tttttggag aagtacgtg ccggtagta 
      ttggtctta taggagatc cacgtaatg ccaacatga 
      taacagcac cagctattc gctctagac ctgaaacgc 
      ccttccagc ttttggggt tgttggcat cggatgtcc 
      tccaagtca ccggtagtg acaattgca ctttcaaca 
      agtctcaga aagagatgg gcaaaaacg acttctcca 
      aaatcccga acggcgtaa acggggtgg aggaccgca 
      tgtcggtca tttgggaga aaggggtgc agtcgggga 
      tgatcgacc cttgtcgtt ttgtcgcaa taactagca 
      caaacgcag ctaaaatct tcaacatct acccgagaa 
      agggctgca ttgcggtcg gatcagacg cagaccttg 
      tcatttggg acccgacca aaactcgaa caatttcgg 
      cctccacgc accaccaag ccgtcgatt tcaacatat 
      tcgaaggga tggtgtgcc atggggtcc ccgagttcg 
      tcattgtga acgggcggg tgtgcgttg acgacggcc 
      agctgcggg tcgtccaag gccacggcc acttcgtgg 
      agacgcccg tctacgccc cgttcgttt acgatcccg 
      agaaaatcg ccgatttga agccggtcc gcaatggga 
      cgcacgatg acaatttgt ccgaacacg ttcacaagg 
      tcacaatcg aggacaaag cctgtccta caccaacac 
      tgccagact cagctgtga gcacgccct cggtcaggg 
      ggccgcgac cggaaggtc aaaggaaca tccaagatt 
      cgacatttt ctatcagcg aggatcttg atgtggaca 
      gaaagtctt gcatccgtg tgaggaacc caccaggtg 
      gcaaatctt ccgggtttt ggtaatcac aagaagacg 
      gcgcctcaa tagttatgc aaattacaa aaaagtacc 
      aaagcaaaa taatcttcc aatcatttc attagaata 
      gttcgtatt tctatttat tacatcatt tacacattt 
      ttctgtctc ttatttgtt caaaaatgt tttatgtta 
      tatcgtaga ttttgttac tgttttttt acattagaa 
      tgtattggg agagtttat gcgtatatg aatatcaaa 
      atcaatgtt gtattgtgc agtaaatga aaaatactg 
      gctattgct gtacgttaa aaatttatc gagacattt 
      gttaatttt ttatttata aaacgcgaa agttaattg 
      catgaaagt agcacagat gtatctgtt ttaagttgc 
      actgtctct gacaactgt tggcttgca gcctgacaa 
      attttcgtt aatactaat ttgtttaat tttattaaa 
      ctactcaga taatcttat attgaaaat gaccatcaa 
      taataaatt ggtcttgtg attttaagt atctcaata 
      ccaaatatc actagtttc gatcattta cgttccatg 
      gactttaca tctaaaggt cattctgtt ctatataag 
      attatgagt ggttataag tttactaat aaacattaa 
      taacaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY