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Gene information
General information
TC010874 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 5048202..5051754
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010874
    iBeetle screen iB_04925

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010874 RA
      MDACFSAFD KNSDGYLDL EEFRLLTRA LFRNDKGKI 
      YNLEEEKLR ETFNVFDGN NDGFIDREE FVFCWNHWI 
      KIQNGLEVV EPINHLLDT VQFDVVFYS LDWHPSNHV 
      SFIDNIGQR EIHPSSPVT AENAQTYDT VIFAGPPPM 
      KQRLWPRHC VQDTWGSEL HKDLKVVEN SIKVYKGTN 
      PDVDSYSVF WDNKKMTDT TLCAQLRMR NATDIYICG 
      LAYDVCVGA TAVDALAIG YRTILLDDC SRGVDLMDI 
      EKTKNTVIK NNGVIINSN QVKAMVEGR DRRPELGYK 
      LAMELKMNR LENNH
    CDS sequence
    • >TC010874 RA
      atggacgcg tgcttctcg gcgtttgat aagaacagt 
      gatggctat ttagatttg gaagagttt cggttgttg 
      actcgtgcc cttttccgg aatgataaa gggaagatc 
      tataattta gaggaagag aagctccga gaaaccttt 
      aacgtgttt gacggcaac aatgacgga ttcattgat 
      agagaagag tttgttttc tgttggaat cactggatc 
      aaaatccaa aatggatta gaagtagtg gaacctatc 
      aatcactta ttggatacc gtccaattc gatgtagta 
      ttctactcc ctagactgg catcctagt aaccacgta 
      tcatttatc gataatatt ggtcaaaga gagatccat 
      ccatcaagt cccgtcaca gctgaaaat gcacaaacc 
      tacgatacg gtcattttc gccgggccg ccccctatg 
      aagcagcgc ctgtggccc cggcactgc gtccaggac 
      acgtggggc tcggaactc cacaaggac ctcaaggtc 
      gtcgagaat tcgataaaa gtgtacaaa ggcaccaat 
      ccagacgtc gattcctat tcggtattt tgggataat 
      aagaaaatg actgatacg actctttgc gcccagttg 
      cgaatgcga aatgcgacg gatatatac atttgcggt 
      ttagcgtat gacgtgtgt gtaggtgcg acggctgtt 
      gatgcactt gcaattggt tacagaacg attctcctc 
      gacgattgc agccgaggg gtcgatttg atggacata 
      gagaagact aaaaacaca gtcattaag aacaacggc 
      gttattatt aattcgaat caagtcaaa gccatggtt 
      gaagggcga gacaggcga ccagagttg ggctataaa 
      ttagcaatg gaactcaaa atgaaccga ctggagaat 
      aatcattag
    mRNA sequence
    • >TC010874 RA
      tgtcagtta ctcattaaa atcgcaaat tgaacttga 
      aattgcaaa ctactttca tttaaataa taaaaatgc 
      ctattgcgt tccaaccct agcgagtcg ctactgaga 
      gtgaataaa agtaaccgt gaggcagta ccactttcg 
      taatattca actgttgca ttatgtgta cgctcttat 
      aataccttt ccaatattc aaatggaca taaagtatt 
      cattcgtcg acctgaaag ctcgagtac aaagagaaa 
      tgactcctc tattattaa acagttctc gccaaatat 
      tagatggac aaaccgcac cccttccac ttaattaat 
      tagaaaaac ataattaca gacttagtg tttattgga 
      ccgtttgcc aaaaaaatg ttgccagta ggtacttga 
      aatttattg catcaactg acgaggaat aaatctcac 
      gactagata aatattttg attggtttc atgtccata 
      gcatattat ttgcgccac tgtgtggta tgtatctat 
      cgatttgcg aagcactta attaattta cacttaggt 
      agccaataa aaccaatta cactattgt cagagttca 
      aggtttaca tttcagcca atatgtcaa agaatcttc 
      atcgctttg gcgcacgca aaatctgta actgcaaat 
      taattatct ttgaatcta taataatta ttttctggt 
      gttctcagt atttttcag ttacgtaaa agcgtcatt 
      tggcacaga aaaaagttt atgcccgtg acagtgtgt 
      aattgtgga atgatacga attgaacat tgcattgta 
      tattgcatt ccaattata ttttcagcg aattttcta 
      tagtacagt tgaaaataa atccgcaat tgtttggcc 
      tcactcgtt ggaaaagtg cttctattt ttgtattcg 
      tttaaccgg ccaaagttg ttttaccgc gttataaaa 
      cattttttt ggttatatc aaggttttt aatttcact 
      tttgtatta caatgcaca caaaactta ttaatttat 
      tccgttttc catcaaata accgaatca agaatgaaa 
      aaaaaaata tccaaaact tagttgtgt cactaatta 
      gtaattaat aataataat tcacaattt cagtatatt 
      ttgacgaaa aaaaaacgt gaggaaaga cgtttctat 
      cgggtttgg gtgctcgta ttttcgcat atttgattg 
      aaataataa agcagctga ggggattgt cgaaagttc 
      aaaaggctt ttagttatt aaaatcggc taaaccaat 
      tcgggaatt acgtttaat taattttag ttcagtgag 
      atttgccgg tgcgtttta taattaaat attaattca 
      aatggaaat gaccgcacg gtgtacctt gtaaatatt 
      ttctaacct tgaacacga taaaaatgt aactgagct 
      ttcgccgga agcaaatgt aatttaaaa aataattgc 
      tgtaggaag cggtttgct tgagaatca ataactttg 
      tgtagaaag catctcttt gatattatt tttattagt 
      gcgaattaa caaagcaaa cgaaataat tttcttttg 
      atgtttttg ccggggata aatacaacg tcacctaaa 
      ggtgtgcga taccgcgtt ccccattgt gctcctaat 
      atggatatt ttcgtggct gaaaggtgc atagaaaac 
      tgtatgggg tagttttta tcgcactac ccggtgcca 
      gtctgtaac acccccgct ttagtgagg accaacaaa 
      ctttgaaaa tgatcgatt tagttgatt ttgtttgct 
      agtgtacca tggacgcgt gcttctcgg cgtttgata 
      agaacagtg atggctatt tagatttgg aagagtttc 
      ggttgttga ctcgtgccc ttttccgga atgataaag 
      ggaagatct ataatttag aggaagaga agctccgag 
      aaaccttta acgtgtttg acggcaaca atgacggat 
      tcattgata gagaagagt ttgttttct gttggaatc 
      actggatca aaatccaaa atggattag aagtagtgg 
      aacctatca atcacttat tggataccg tccaattcg 
      atgtagtat tctactccc tagactggc atcctagta 
      accacgtat catttatcg ataatattg gtcaaagag 
      agatccatc catcaagtc ccgtcacag ctgaaaatg 
      cacaaacct acgatacgg tcattttcg ccgggccgc 
      cccctatga agcagcgcc tgtggcccc ggcactgcg 
      tccaggaca cgtggggct cggaactcc acaaggacc 
      tcaaggtcg tcgagaatt cgataaaag tgtacaaag 
      gcaccaatc cagacgtcg attcctatt cggtatttt 
      gggataata agaaaatga ctgatacga ctctttgcg 
      cccagttgc gaatgcgaa atgcgacgg atatataca 
      tttgcggtt tagcgtatg acgtgtgtg taggtgcga 
      cggctgttg atgcacttg caattggtt acagaacga 
      ttctcctcg acgattgca gccgagggg tcgatttga 
      tggacatag agaagacta aaaacacag tcattaaga 
      acaacggcg ttattatta attcgaatc aagtcaaag 
      ccatggttg aagggcgag acaggcgac cagagttgg 
      gctataaat tagcaatgg aactcaaaa tgaaccgac 
      tggagaata atcattagt taccttcta atatataaa 
      tagttattg ccaatgtgg acgattaaa atttaatga 
      gcgaaaaaa gtgttccag cgccgggat ttgcttttg 
      tgggtatat atgagtgtg taaaaaggc aatttgtgc 
      ataattgta ttgaaattg tatttgaat gaaataaat 
      tggtaagaa gctttgaag agtctttaa tttttaata 
      aaaaacggt ttttgaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY