Status
Please wait ...
Gene information
General information
TC010856 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 5505991..5515095
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010856
    iBeetle screen iB_04922

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010856 RA
      MFPEDTFAI CDPRRDEDC RKNGDKEVH PKINGNRRN 
      SCAIVTNTC HTCNGYYGP CFDDPVCST CHAFLYPNT 
      APIMDRPST FQRSSDSDS GNDEPVDNS QESMPNVPL 
      YEDFVGKMA PVRVDTLTK EIQRLSNVT HHVTDLNLE 
      LFPPEVLMH MFKYLDEIS LWSIGQVCS RWREILFMC 
      VKLERWRDF VRLRWPLLP LYDYEQDWY ELYTAMMLS 
      SCCFKCIKQ MAAQKLPSH WEDSSWRRH RLRTELKAM 
      RSDPLSGID AFPLDAHCC HWQATIEGP AGSPYEGGL 
      FFLYIQVPT TYPLDPPLV RFITKIFHP NVSRHGDIG 
      IDSIHHNWS LALTLSKLL ISIQSLLTD PFCDVCMEP 
      EIGNMFLKN REAFELEAK MWTCKHAMH DMLPH
    CDS sequence
    • >TC010856 RA
      atgtttcct gaagatacg tttgcaatt tgtgatcca 
      cgacgtgat gaagactgt cgcaagaac ggggacaag 
      gaggtgcac ccgaaaatc aacggaaat cggagaaat 
      tcctgcgcg attgtaaca aatacgtgc cacacgtgc 
      aatgggtat tatgggccg tgctttgac gaccctgtg 
      tgctccacc tgccacgct tttctgtac cccaacact 
      gcccccatt atggacagg ccctcaacc ttccaaaga 
      tccagtgat agtgattcg ggcaatgac gagccggtg 
      gataacagt caggaaagc atgcccaac gtccccctt 
      tacgaggac ttcgtgggc aaaatggcc cccgtaagg 
      gtggatacc ctgactaaa gaaatacaa aggctctca 
      aacgtcact catcacgta actgacttg aatttagag 
      ctatttcca ccggaagtt cttatgcat atgtttaaa 
      tatttagac gaaatttcg ctgtggagc ataggtcaa 
      gtttgtagc aggtggcgg gagatactt ttcatgtgt 
      gtgaagctc gagcgttgg cgagacttt gtgcgctta 
      aggtggccg ttgttgcct ttatatgat tacgaacaa 
      gactggtat gaactttat acagccatg atgcttagc 
      tcgtgttgt tttaaatgt atcaaacaa atggccgct 
      caaaaattg ccctcgcat tgggaggac agttcctgg 
      agacgacac cgtttaaga accgagctt aaagcaatg 
      aggagtgat cctctcagt gggattgat gcgtttcca 
      cttgatgct cattgttgc cattggcag gcgacgatt 
      gagggacca gcgggcagt ccttacgag ggcggattg 
      ttctttctg tacattcaa gtaccaaca acttaccca 
      ttagatcct ccacttgtt aggtttatc acgaaaatt 
      tttcatccc aacgtttca agacacgga gacattggt 
      atcgattca attcatcac aactggtca ttagctctc 
      acacttagc aaattgttg atttctata cagtcactt 
      cttaccgac cctttctgc gatgtttgt atggaaccc 
      gaaataggg aatatgttc ctcaaaaac agggaggcg 
      ttcgaactt gaagctaag atgtggact tgcaaacat 
      gcaatgcat gatatgttg ccacactga
    mRNA sequence
    • >TC010856 RA
      taaaaaata ataaatctg aattaagga aactaaacc 
      gcacggaaa tcgaacatt taaatgatt gcatgtgaa 
      gtaacagcg attttaagg taaagtgtg gcaatagtg 
      agtgaacaa ctagcccaa aatacccac ctgaaactt 
      gactaattg attgcattt tcctgtatt tcggtgcaa 
      attagcatt aatttgcat tgaatgtgg gtgttttaa 
      tttaccaaa actcagggg agagatgaa tgcattggg 
      aatttgata aattttcta cgaagtggg gataataca 
      aattagttt tatagactc ttctaacct cgtgtgttg 
      tggtccact gtcatgaac ataatacac tcaaccttg 
      tcgctctct taagaatat ttgtttgtt taacaaaag 
      tgactaatc cgaaatgac catttacgg caagttagt 
      gcaaataat attagtgaa ttattttaa gtgaagtaa 
      gtgccggtt ttagtgata ctttgagtt aaaaacgct 
      tctgtttca ggaagatgt gttcgggtg ctgtgaaca 
      tcccaatta aatctccat atttttaat tattaatta 
      agtatgaat ttatttttg taaataaag tttattaac 
      ttaatgccg tcataaatt gataatgtt attattatt 
      gtggttttt gttagttat ggaagagta gaacagaaa 
      attcgataa gttaattat tttttaata atttacact 
      cggttttat aaccgttga aacgtattt atgtttggt 
      tataacctg cataacctt acttttgga aatgtttta 
      ttttggaaa aatcgcatt tgcatgtgc tgttgtttt 
      tttcctgtc aatgtcaaa caaatttta atttgacac 
      aattcacat caaaattgg gaatgcgct agaaaaaac 
      catgtttcc tgaagatac gtttgcaat ttgtgatcc 
      acgacgtga tgaagactg tcgcaagaa cggggacaa 
      ggaggtgca cccgaaaat caacggaaa tcggagaaa 
      ttcctgcgc gattgtaac aaatacgtg ccacacgtg 
      caatgggta ttatgggcc gtgctttga cgaccctgt 
      gtgctccac ctgccacgc ttttctgta ccccaacac 
      tgcccccat tatggacag gccctcaac cttccaaag 
      atccagtga tagtgattc gggcaatga cgagccggt 
      ggataacag tcaggaaag catgcccaa cgtccccct 
      ttacgagga cttcgtggg caaaatggc ccccgtaag 
      ggtggatac cctgactaa agaaataca aaggctctc 
      aaacgtcac tcatcacgt aactgactt gaatttaga 
      gctatttcc accggaagt tcttatgca tatgtttaa 
      atatttaga cgaaatttc gctgtggag cataggtca 
      agtttgtag caggtggcg ggagatact tttcatgtg 
      tgtgaagct cgagcgttg gcgagactt tgtgcgctt 
      aaggtggcc gttgttgcc tttatatga ttacgaaca 
      agactggta tgaacttta tacagccat gatgcttag 
      ctcgtgttg ttttaaatg tatcaaaca aatggccgc 
      tcaaaaatt gccctcgca ttgggagga cagttcctg 
      gagacgaca ccgtttaag aaccgagct taaagcaat 
      gaggagtga tcctctcag tgggattga tgcgtttcc 
      acttgatgc tcattgttg ccattggca ggcgacgat 
      tgagggacc agcgggcag tccttacga gggcggatt 
      gttctttct gtacattca agtaccaac aacttaccc 
      attagatcc tccacttgt taggtttat cacgaaaat 
      ttttcatcc caacgtttc aagacacgg agacattgg 
      tatcgattc aattcatca caactggtc attagctct 
      cacacttag caaattgtt gatttctat acagtcact 
      tcttaccga ccctttctg cgatgtttg tatggaacc 
      cgaaatagg gaatatgtt cctcaaaaa cagggaggc 
      gttcgaact tgaagctaa gatgtggac ttgcaaaca 
      tgcaatgca tgatatgtt gccacactg aatatttat 
      ttatctatt gtttttttt tcgcaaaat tatgattga 
      gttaatcaa caacatgcc gtgcatgta tgttgccgc 
      aaagggcat ttgttttta tttaatttt tttttgcta 
      aatttttat ggtggcaat gtcaattat gctttttat 
      ttctcaaaa tttattttg atttgtaag aattgagaa 
      catgccatg catgatgtg ttggcatac tcatatata 
      tttatttta tttactttt attttttgc aaaatcatg 
      tttgtgaca aggccaatt tattgatat tttacctta 
      tttgttaca ttatatttt ttgtgattt gtgttcaat 
      aaacctgtt tcttaaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY