Status
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Gene information
General information
TC010853 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 5526626..5529145
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010853
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04921

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010853 RA
      MAVPSRARV YADVNSHKP REYWDYESY VVDWGQQDD 
      YQLVRKLGR GKYSEVFEA VNVTNNEKC VVKILKPVK 
      KKKIKREIK ILENLRGGT NIITLEAVV KDPVSRTPA 
      LIFEHVNNT DFKQLYQTL TDFDIRYYL YELLKALDY 
      CHSMGIMHR DVKPHNVMI DHENRKLRL IDWGLAEFY 
      HPGQEYNVR VASRYFKGP ELLVDYQMY DYSLDMWSL 
      GCMLASMIF RKEPFFHGH DNYDQLVRI AKVLGTEEL 
      FEYLDKYHI ELDPRFNDI LGRHSRKRW ERFVHNENQ 
      HLVSAEALD FLDKLLRYD HLERLTARD AMDHSYFYP 
      VVKEQCRMM STVSSSPTP LTGNLSVVG VGE
    • >TC010853 RB
      MAVPSRARV YADVNSHKP REYWDYESY VVDWGQQDD 
      YQLVRKLGR GKYSEVFEA VNVTNNEKC VVKILKPVK 
      KKKIKREIK ILENLRGGT NIITLEAVV KDPVSRTPA 
      LIFEHVNNT DFKQLYQTL TDFDIRYYL YELLKALDY 
      CHSMGIMHR DVKPHNVMI DHENRKLRL IDWGLAEFY 
      HPGQEYNVR VASRYFKGP ELLVDYQMY DYSLDMWSL 
      GCMLASMIF RKEPFFHGH DNYDQLVRI AKVLGTEEL 
      FEYLDKYHI ELDPRFNDI LGRHSRKRH SRKRWERFV 
      HNENQHLVS AEALDFLDK LLRYDHLER LTARDAMDH 
      SYFYPVVKE QCRMMSTVS SSPTPLTGN LSVVGVGE
    CDS sequence
    • >TC010853 RA
      atggcagta cctagtcgt gcccgagtt tatgccgat 
      gttaattcg cacaaacca cgagaatat tgggactac 
      gaaagctac gttgtggac tggggccaa caggacgat 
      taccagttg gtcaggaag ctaggaaga gggaaatat 
      agcgaagtt ttcgaagcc gtcaatgtt acgaacaat 
      gagaaatgt gtagtcaaa atattaaag ccagtgaag 
      aagaagaaa attaaacga gaaatcaag atattagag 
      aatttacgt ggaggtaca aatattatt actcttgag 
      gctgttgtg aaagatccg gtatcaaga acaccagcg 
      cttattttc gaacacgtc aataacaca gatttcaag 
      cagctttac caaacgctt acagacttc gatattcgt 
      tactattta tatgagttg ctcaaagct ttggattat 
      tgtcacagt atgggtatt atgcatagg gatgttaaa 
      ccacataat gtaatgatt gatcatgaa aatagaaag 
      ttaaggcta attgattgg ggtttagca gaattttat 
      catccagga caagaatac aatgtaagg gtcgcttct 
      aggtacttt aaaggacct gaactgctt gtagactac 
      caaatgtat gattattca ttagatatg tggtcattg 
      ggatgtatg ttagcttca atgatattt agaaaagag 
      cctttcttt catggccat gataattac gaccaatta 
      gtccgtatt gccaaagtc ctaggaact gaagagctt 
      ttcgaatac cttgataaa taccacata gaactagac 
      ccaaggttt aacgacatt ttaggaaga cattcccgt 
      aagaggtgg gagcgattc gtgcataat gaaaaccag 
      cacctagtt tcggcggaa gcgttagac tttttggat 
      aaactgttg cgttacgat catctagag cgtctcacc 
      gcacgtgac gccatggat cactcctat ttctaccct 
      gttgttaag gaacagtgc cgcatgatg tcaacagta 
      tcttcatcc cctactcca cttacaggc aatctgtct 
      gttgtagga gtaggtgag tag
    • >TC010853 RB
      atggcagta cctagtcgt gcccgagtt tatgccgat 
      gttaattcg cacaaacca cgagaatat tgggactac 
      gaaagctac gttgtggac tggggccaa caggacgat 
      taccagttg gtcaggaag ctaggaaga gggaaatat 
      agcgaagtt ttcgaagcc gtcaatgtt acgaacaat 
      gagaaatgt gtagtcaaa atattaaag ccagtgaag 
      aagaagaaa attaaacga gaaatcaag atattagag 
      aatttacgt ggaggtaca aatattatt actcttgag 
      gctgttgtg aaagatccg gtatcaaga acaccagcg 
      cttattttc gaacacgtc aataacaca gatttcaag 
      cagctttac caaacgctt acagacttc gatattcgt 
      tactattta tatgagttg ctcaaagct ttggattat 
      tgtcacagt atgggtatt atgcatagg gatgttaaa 
      ccacataat gtaatgatt gatcatgaa aatagaaag 
      ttaaggcta attgattgg ggtttagca gaattttat 
      catccagga caagaatac aatgtaagg gtcgcttct 
      aggtacttt aaaggacct gaactgctt gtagactac 
      caaatgtat gattattca ttagatatg tggtcattg 
      ggatgtatg ttagcttca atgatattt agaaaagag 
      cctttcttt catggccat gataattac gaccaatta 
      gtccgtatt gccaaagtc ctaggaact gaagagctt 
      ttcgaatac cttgataaa taccacata gaactagac 
      ccaaggttt aacgacatt ttaggaaga cattcccgt 
      aagagacat tcccgtaag aggtgggag cgattcgtg 
      cataatgaa aaccagcac ctagtttcg gcggaagcg 
      ttagacttt ttggataaa ctgttgcgt tacgatcat 
      ctagagcgt ctcaccgca cgtgacgcc atggatcac 
      tcctatttc taccctgtt gttaaggaa cagtgccgc 
      atgatgtca acagtatct tcatcccct actccactt 
      acaggcaat ctgtctgtt gtaggagta ggtgagtag
    mRNA sequence
    • >TC010853 RA
      tctaccgtg gcgggatgg aagttcctt ccatcttgg 
      ttctgactc cttgctaag aagccgggt agtgttgag 
      gaaaaaaaa catatatag aattttaga taaaaagta 
      aaaggttat cggagttgc tatgtgtcg ttttatcgg 
      atatttcta tatataagt tgtctacgg aatgaacta 
      gtacccgtt caaggaact cctcgagct tatgttcgt 
      tctttcgtg agcagcctt gtgcataat aaaatggca 
      gtacctagt cgtgcccga gtttatgcc gatgttaat 
      tcgcacaaa ccacgagaa tattgggac tacgaaagc 
      tacgttgtg gactggggc caacaggac gattaccag 
      ttggtcagg aagctagga agagggaaa tatagcgaa 
      gttttcgaa gccgtcaat gttacgaac aatgagaaa 
      tgtgtagtc aaaatatta aagccagtg aagaagaag 
      aaaattaaa cgagaaatc aagatatta gagaattta 
      cgtggaggt acaaatatt attactctt gaggctgtt 
      gtgaaagat ccggtatca agaacacca gcgcttatt 
      ttcgaacac gtcaataac acagatttc aagcagctt 
      taccaaacg cttacagac ttcgatatt cgttactat 
      ttatatgag ttgctcaaa gctttggat tattgtcac 
      agtatgggt attatgcat agggatgtt aaaccacat 
      aatgtaatg attgatcat gaaaataga aagttaagg 
      ctaattgat tggggttta gcagaattt tatcatcca 
      ggacaagaa tacaatgta agggtcgct tctaggtac 
      tttaaagga cctgaactg cttgtagac taccaaatg 
      tatgattat tcattagat atgtggtca ttgggatgt 
      atgttagct tcaatgata tttagaaaa gagcctttc 
      tttcatggc catgataat tacgaccaa ttagtccgt 
      attgccaaa gtcctagga actgaagag cttttcgaa 
      taccttgat aaataccac atagaacta gacccaagg 
      tttaacgac attttagga agacattcc cgtaagagg 
      tgggagcga ttcgtgcat aatgaaaac cagcaccta 
      gtttcggcg gaagcgtta gactttttg gataaactg 
      ttgcgttac gatcatcta gagcgtctc accgcacgt 
      gacgccatg gatcactcc tatttctac cctgttgtt 
      aaggaacag tgccgcatg atgtcaaca gtatcttca 
      tcccctact ccacttaca ggcaatctg tctgttgta 
      ggagtaggt gagtagcta ccaggtaaa gcactttgt 
      catttcaca tcatgccat actctgttg taattagtc 
      actacattt ttattcata tttcgatgc tatattgta 
      tatcgcagt ttttctgaa tatgtttgc aagttttgt 
      gtattaatt aaacgattc ttttgtac
    • >TC010853 RB
      tctaccgtg gcgggatgg aagttcctt ccatcttgg 
      ttctgactc cttgctaag aagccgggt agtgttgag 
      gaaaaaaaa catatatag aattttaga taaaaagta 
      aaaggttat cggagttgc tatgtgtcg ttttatcgg 
      atatttcta tatataagt tgtctacgg aatgaacta 
      gtacccgtt caaggaact cctcgagct tatgttcgt 
      tctttcgtg agcagcctt gtgcataat aaaatggca 
      gtacctagt cgtgcccga gtttatgcc gatgttaat 
      tcgcacaaa ccacgagaa tattgggac tacgaaagc 
      tacgttgtg gactggggc caacaggac gattaccag 
      ttggtcagg aagctagga agagggaaa tatagcgaa 
      gttttcgaa gccgtcaat gttacgaac aatgagaaa 
      tgtgtagtc aaaatatta aagccagtg aagaagaag 
      aaaattaaa cgagaaatc aagatatta gagaattta 
      cgtggaggt acaaatatt attactctt gaggctgtt 
      gtgaaagat ccggtatca agaacacca gcgcttatt 
      ttcgaacac gtcaataac acagatttc aagcagctt 
      taccaaacg cttacagac ttcgatatt cgttactat 
      ttatatgag ttgctcaaa gctttggat tattgtcac 
      agtatgggt attatgcat agggatgtt aaaccacat 
      aatgtaatg attgatcat gaaaataga aagttaagg 
      ctaattgat tggggttta gcagaattt tatcatcca 
      ggacaagaa tacaatgta agggtcgct tctaggtac 
      tttaaagga cctgaactg cttgtagac taccaaatg 
      tatgattat tcattagat atgtggtca ttgggatgt 
      atgttagct tcaatgata tttagaaaa gagcctttc 
      tttcatggc catgataat tacgaccaa ttagtccgt 
      attgccaaa gtcctagga actgaagag cttttcgaa 
      taccttgat aaataccac atagaacta gacccaagg 
      tttaacgac attttagga agacattcc cgtaagaga 
      cattcccgt aagaggtgg gagcgattc gtgcataat 
      gaaaaccag cacctagtt tcggcggaa gcgttagac 
      tttttggat aaactgttg cgttacgat catctagag 
      cgtctcacc gcacgtgac gccatggat cactcctat 
      ttctaccct gttgttaag gaacagtgc cgcatgatg 
      tcaacagta tcttcatcc cctactcca cttacaggc 
      aatctgtct gttgtagga gtaggtgag tagctacca 
      ggtaaagca ctttgtcat ttcacatca tgccatact 
      ctgttgtaa ttagtcact acattttta ttcatattt 
      cgatgctat attgtatat cgcagtttt tctgaatat 
      gtttgcaag ttttgtgta ttaattaaa cgattcttt 
      tgtac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY