Status
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Gene information
General information
TC010850 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 5548024..5561803
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010850
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04920

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 9 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010850 RA
      MGRKKIQIS RITDERNRQ VTFNKRKFG VMKKAYELS 
      VLCDCEIAL IIFSSSNKL YQYASTDMD KVLLKYTEY 
      NEPHESLTN KNIIEALNK KEHKNGVMS PDSPEPETE 
      YQLTPRTEA KYSKIDEEF QMMLQRNQI NGQRVSMSS 
      SNYTQPVSV PVNISYGDS GLLQSSPQM AHTSISPRP 
      SSSETDSVY PSGGMPEMS NGYPNSASP MDGSPSPGP 
      SPGPGLSSS KQHPKQHSP GPRSNLRVV IPTALGPSI 
      TPEEVSYGE HNRTQSTLN TPVVALQTP GLSNYPGTL 
      SSFGAQDFS MGADMGLSS ISWSGHQIS TSLTHTSNC 
      LPHLAVSSS TPPPSSSSP IPVKIKSEP ISPPREHHP 
      HLSVGVHHS HVQSLNLTH SHHPRPSSA GHITPTPGS 
      VTPTNLPSP TGPPSGGDY ENIQMHKRA RLSDWPS
    CDS sequence
    • >TC010850 RA
      atgggtcgc aaaaaaata caaatatca agaatcacc 
      gatgagcgg aatcgacag gtcactttc aacaagcgg 
      aagttcggc gtgatgaag aaagcttac gaactctcg 
      gttttgtgt gactgcgag atcgcgctt atcatcttc 
      agcagcagc aacaaattg taccagtac gcgagtacg 
      gacatggac aaagtgctg ctgaaatac accgaatac 
      aacgagccc catgagtct ctcaccaac aaaaatatt 
      atcgaggca cttaataag aaagagcat aagaacgga 
      gtgatgagt cctgacagt cccgagccg gagacggag 
      taccagttg acgccgcgg accgaggcc aagtacagc 
      aaaatcgac gaggaattt caaatgatg cttcaaaga 
      aaccaaatc aatggacag agggttagc atgagctct 
      tcgaattat acgcaaccc gtatcggtg cctgttaat 
      ataagttat ggtgattct ggtcttctt caatctagt 
      ccgcaaatg gctcatact agtattagt ccccgaccg 
      tcttcttct gaaacagat tcggtttat ccgagcgga 
      ggaatgccc gagatgagc aatggttat ccaaattca 
      gcatctcca atggatgga tcaccgtcc ccaggacca 
      agtccagga ccaggtctt agttcctca aagcagcat 
      ccaaaacaa cattcaccc ggtccgcga tcgaacctc 
      cgagtcgtc atccctacg gcactagga ccaagtatt 
      accccggaa gaagtttca tatggagaa cacaatcgg 
      actcaatcg actctgaat acccccgtg gtggcgctg 
      cagactccc ggtttgtcg aattacccc ggtactttg 
      agttcgttc ggcgctcag gatttcagt atgggggcc 
      gatatgggc ttgagttca atatcctgg agcggtcat 
      cagatttcg acgtctttg acacacaca agtaactgc 
      ttgccgcat ttggcagtg tcgagcagc acaccaccg 
      ccgtcgtca tcttcaccg atacctgtg aaaataaag 
      agtgaaccg atatcgcct cctcgagaa catcatccg 
      catctgagt gtgggtgtc caccattcg cacgtacag 
      agcttgaac ttgacgcac agtcaccat ccgaggccc 
      agttctgcc ggtcatata acgccgact cctggaagt 
      gtcacgccg acgaatttg ccgtcgccg acggggccg 
      ccaagcggg ggtgattac gagaatatt cagatgcat 
      aagcgagca agactctca gactggcca tcctag
    mRNA sequence
    • >TC010850 RA
      cccgtcgga caactgctt cgacgggcc atgcctccc 
      caaagggcc gtaaggtta ccgaaaaaa atgccgtcg 
      ccgcgttgg cccaaaatc cgtgtgctg tgaagtgct 
      gtgtgaatt cgggaaaca tatgacgct cgcttcccc 
      ttacagccg atcccctgg agacacaag gatttaaag 
      cacctacca agatctcta cttacccgt caatcccac 
      acccacgat cattaacga ccaacctag aggtgatac 
      tccagtctt tgatacgtc agcgcttta ggctattgc 
      cattgtgta agtcgacac gggttttat atcttgtga 
      ttcaaaatt gctgccatg ggtcgcaaa aaaatacaa 
      atatcaaga atcaccgat gagcggaat cgacaggtc 
      actttcaac aagcggaag ttcggcgtg atgaagaaa 
      gcttacgaa ctctcggtt ttgtgtgac tgcgagatc 
      gcgcttatc atcttcagc agcagcaac aaattgtac 
      cagtacgcg agtacggac atggacaaa gtgctgctg 
      aaatacacc gaatacaac gagccccat gagtctctc 
      accaacaaa aatattatc gaggcactt aataagaaa 
      gagcataag aacggagtg atgagtcct gacagtccc 
      gagccggag acggagtac cagttgacg ccgcggacc 
      gaggccaag tacagcaaa atcgacgag gaatttcaa 
      atgatgctt caaagaaac caaatcaat ggacagagg 
      gttagcatg agctcttcg aattatacg caacccgta 
      tcggtgcct gttaatata agttatggt gattctggt 
      cttcttcaa tctagtccg caaatggct catactagt 
      attagtccc cgaccgtct tcttctgaa acagattcg 
      gtttatccg agcggagga atgcccgag atgagcaat 
      ggttatcca aattcagca tctccaatg gatggatca 
      ccgtcccca ggaccaagt ccaggacca ggtcttagt 
      tcctcaaag cagcatcca aaacaacat tcacccggt 
      ccgcgatcg aacctccga gtcgtcatc cctacggca 
      ctaggacca agtattacc ccggaagaa gtttcatat 
      ggagaacac aatcggact caatcgact ctgaatacc 
      cccgtggtg gcgctgcag actcccggt ttgtcgaat 
      taccccggt actttgagt tcgttcggc gctcaggat 
      ttcagtatg ggggccgat atgggcttg agttcaata 
      tcctggagc ggtcatcag atttcgacg tctttgaca 
      cacacaagt aactgcttg ccgcatttg gcagtgtcg 
      agcagcaca ccaccgccg tcgtcatct tcaccgata 
      cctgtgaaa ataaagagt gaaccgata tcgcctcct 
      cgagaacat catccgcat ctgagtgtg ggtgtccac 
      cattcgcac gtacagagc ttgaacttg acgcacagt 
      caccatccg aggcccagt tctgccggt catataacg 
      ccgactcct ggaagtgtc acgccgacg aatttgccg 
      tcgccgacg gggccgcca agcgggggt gattacgag 
      aatattcag atgcataag cgagcaaga ctctcagac 
      tggccatcc tagaccggc ggcggcggt ggcggcggc 
      ggcgcacaa gacgctaag tgccatagt gataacgtg 
      tggataacc gaacgcgtt tctattgcc aattgtcat 
      ctatgtgat aaactacga gtctagtaa aactggata 
      ttttatgaa atttgtagt catttatca gtgaaacat 
      acataaaaa cgattttgt agagagaaa gggagagag 
      agcgcagcg cgagagagg atcagattt atatagaat 
      gggttagtg tggtgagtg tgtcgtggt tgttccagt 
      cagcgacag cgttatatg ctggcgcca acatttgtt 
      ctcagaaac aaacataat atatagtgc cattatttt 
      cttgtaatt attagtgtc ttttttata aatgttttt 
      agataaata gtatattat ttataatgt acacttgtt 
      aatttagcg aggatgcag ccactaaca aacatattg 
      agactatgc aggacacta ctcttgtta tgtattggt 
      gctttcccc aacctgatc ggatgtact ctgtccact 
      ggctgttgg tgcggacgg cttgacaga cgccgcggc 
      ggctctgct tcgccggat tgacgctgg ccgtcggtc 
      cgtccgtcc gtccgcccg tcaatgttg aatgtactg 
      tatttgtac tgcttcccc attggaacc tagagtgtt 
      cattgttgt agccttttc aatttgttt gtgatttca 
      gagtacgat atctattct gtatattga cctacacaa 
      attaaatta tataattat acattatat aatatatag 
      atatatttt atctactgt tgtgtgcac aaaccttgc 
      acatagttt tatagttta aatgtcggt tcgacccgg 
      ctgtccgtc ttcgcacgg atgacccta tctccactg 
      aactactgt aggattgcc ttactcaca gtgcctcga 
      cagtatgta cgtatctat ttgtgtact acaccattg 
      gatgtcttc agtaaaagc catcttaag tccgaatgc 
      gctgcggta cattttcgt cgataattc acagggtat 
      tgcgccgtt ctacactcc actctgctg actggcacg 
      tgctagtgc tagtgctag acagctgcc gctgcttgt 
      gccttcttg ttaacctgg tacctttcc gaaccccca 
      actggcttc cgtgtcact gcactactg tctcttctg 
      tatgttatt ctttattta tgtcaatgt tttgacatg 
      tcagttgac acgatttta tttagtcgt tttatgtta 
      agcggaaca ttacgcctc atttttaaa tggacgaaa 
      tacccagct acattatgt aaatatagt tatatttta 
      ttgtagcgt tgccaatat ttgttgatc attttacac 
      ctcatcttt cattttaca tttacatca taaggcatt 
      atattacgt ccatttgac ataggcatt tttcaatca 
      tgtgcaata ttaatctga cgtttattt atctatgat 
      gtattgaat taaaccaaa taaaaaatt gtttgttac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY