Status
Please wait ...
Gene information
General information
TC010848 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 5681276..5688364
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010848
    iBeetle screen iB_04919

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    vitellarium not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (22 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010848 RA
      MVGLSTGHI FSTSSSKEV GDMSGLATT APSSADACL 
      SIVHSLMCH RQGGESEGF AKRAIESLV KKLKEKRDE 
      LDSLITAIT TSGAHPSKC VTIQRTLDG RLQVAGRKG 
      FPHVIYARI WRWPDLHKN ELKHVKYCQ FAFDLKCDS 
      VCVNPYHYE RVVSPGIDL SGLTLQSGT ARLVKDEYT 
      AGPVPGGSM DVDSEIGIE VSQTIQHQP PQQNFALSG 
      LQAPSTSEQ GMYNSPNRP VMGHPVPKI EGGVDACSR 
      SSWLPHMHI PHRGSNPPV SPHLQQNGF NNSTHTTTA 
      VVNNNGGTI SGSQPAATA TMGQSFTGA GGTWTGSNT 
      LTYTQSMQP PDNRTHHTA YWNTNQCND VNIAGLLST 
      QPAPEYWCS VAYFELDTQ VGETFKVPS SCPNVTIDG 
      YVDPSGGNR FCLGALSNV HRTDQSERA RLHIGKGVQ 
      LDLRGEGDV WLRCLSDHS VFVQSYYLD REAGRQPGD 
      AVHKIYPSA YIKVFDLRQ CHNQMTTQA ATAQAAAAA 
      QAAAVAGHI PGPHSVGGI APAISLSAA TGIGVDDLR 
      RLCILRLSF VKGWGPDYP RQSIKETPC WVEIHLHRA 
      LQLLDEVLH TMPIDGPRG IE
    • >TC010848 RB
      MRPQVGDMS GLATTAPSS ADACLSIVH SLMCHRQGG 
      ESEGFAKRA IESLVKKLK EKRDELDSL ITAITTSGA 
      HPSKCVTIQ RTLDGRLQV AGRKGFPHV IYARIWRWP 
      DLHKNELKH VKYCQFAFD LKCDSVCVN PYHYERVVS 
      PGIDLSGLT LQSGTARLV KDEYTAGPV PGGSMDVDS 
      EIGIEVSQT IQHQPPQQN FALSGLQAP STSEQGMYN 
      SPNRPVMGH PVPKIEGGV DACSRSSWL PHMHIPHRG 
      SNPPVSPHL QQNGFNNST HTTTAVVNN NGGTISGSQ 
      PAATATMGQ SFTGAGGTW TGSNTLTYT QSMQPPDNR 
      THHTAYWNT NQCNDVNIA GLLSTQPAP EYWCSVAYF 
      ELDTQVGET FKVPSSCPN VTIDGYVDP SGGNRFCLG 
      ALSNVHRTD QSERARLHI GKGVQLDLR GEGDVWLRC 
      LSDHSVFVQ SYYLDREAG RQPGDAVHK IYPSAYIKV 
      FDLRQCHNQ MTTQAATAQ AAAAAQAAA VAGHIPGPH 
      SVGGIAPAI SLSAATGIG VDDLRRLCI LRLSFVKGW 
      GPDYPRQSI KETPCWVEI HLHRALQLL DEVLHTMPI 
      DGPRGIE
    CDS sequence
    • >TC010848 RA
      atggtgggg ctttccacg ggacacata ttctccaca 
      agttcgtcg aaagaagtt ggagatatg tcagggtta 
      gccacaaca gcaccctca tcggctgac gcttgcctg 
      agcattgtg cacagtctc atgtgccac agacaaggg 
      ggagaaagc gaaggcttc gccaaacgt gctatagag 
      tccctggtg aaaaagcta aaagagaaa agggatgaa 
      ctagactcg cttatcact gctataacc accagtggg 
      gcacatccg agcaagtgc gtcacgatc cagaggact 
      ctcgacggc cgcctgcag gttgccgga cgcaagggc 
      tttccgcac gttatctac gctcgaatc tggcgatgg 
      cccgacctc cacaaaaac gaactcaaa cacgtgaaa 
      tattgtcag tttgcgttt gatttaaaa tgtgactca 
      gtatgtgtg aatccgtac cattacgaa cgggtagtg 
      tcccccggc attgacctc tctggactt acgttgcaa 
      tctggaacc gctcgatta gtgaaagat gagtacacc 
      gctggaccg gtccctgga ggcagtatg gacgtagac 
      agcgaaatc ggcattgaa gtctcccag accatccaa 
      caccagccc ccacagcag aatttcgcc ctgagcgga 
      cttcaggca ccttccaca agtgaacag ggaatgtat 
      aattcacca aacagacca gttatggga catcccgta 
      cctaaaatt gaaggggga gttgacgca tgttccaga 
      tctagttgg ttaccgcac atgcatata cctcatcgt 
      gggagtaac cctcccgtg tcgccccat ttgcaacaa 
      aacgggttc aacaactcc acccatacg acaactgct 
      gttgttaac aacaacggt ggtaccata agcggcagc 
      caacccgcg gctacggct accatgggc caatcgttc 
      actggggct ggtggtact tggacagga tctaatacg 
      ttaacctac acccaatca atgcagccc cctgataac 
      cgcacccat cacacagct tactggaac acaaaccaa 
      tgcaacgac gtaaacatc gctggtctg ttgtccacc 
      cagccagcc cccgagtac tggtgttcc gtcgcctac 
      ttcgagctg gacactcaa gtgggtgaa acgttcaaa 
      gtaccctca agctgcccg aatgtgaca attgacggc 
      tacgtcgac ccctctgga ggcaaccgg ttttgcctt 
      ggtgcgttg agtaatgtg cacaggacg gaccaaagc 
      gagagagcg agactgcac attggcaag ggggttcag 
      ttagattta aggggcgaa ggcgacgtt tggttgcgc 
      tgcttaagt gaccattcc gttttcgtc cagagttat 
      tatctggat agggaggct ggacgacaa cccggcgat 
      gccgtacac aaaatttac ccgtcagcc tacataaag 
      gtctttgat ctaaggcag tgtcacaac cagatgaca 
      actcaggca gccacagca caggcagcg gcggcggca 
      caggcggcg gcggtggcg gggcacatc cctggaccg 
      cactcagtc ggcgggata gcgccagcc ataagtttg 
      tcggcagcc acgggaata ggggttgac gatttgcga 
      agactgtgc atcctgcgc ctcagtttt gtgaaaggt 
      tggggcccg gattatcct cgacagtcc attaaagaa 
      acgccttgt tgggttgaa atccacctg caccgcgct 
      ctgcaactg ctggacgaa gtgcttcat actatgccg 
      attgatgga ccccgtggg atagagtaa
    • >TC010848 RB
      atgcgtccc caagttgga gatatgtca gggttagcc 
      acaacagca ccctcatcg gctgacgct tgcctgagc 
      attgtgcac agtctcatg tgccacaga caaggggga 
      gaaagcgaa ggcttcgcc aaacgtgct atagagtcc 
      ctggtgaaa aagctaaaa gagaaaagg gatgaacta 
      gactcgctt atcactgct ataaccacc agtggggca 
      catccgagc aagtgcgtc acgatccag aggactctc 
      gacggccgc ctgcaggtt gccggacgc aagggcttt 
      ccgcacgtt atctacgct cgaatctgg cgatggccc 
      gacctccac aaaaacgaa ctcaaacac gtgaaatat 
      tgtcagttt gcgtttgat ttaaaatgt gactcagta 
      tgtgtgaat ccgtaccat tacgaacgg gtagtgtcc 
      cccggcatt gacctctct ggacttacg ttgcaatct 
      ggaaccgct cgattagtg aaagatgag tacaccgct 
      ggaccggtc cctggaggc agtatggac gtagacagc 
      gaaatcggc attgaagtc tcccagacc atccaacac 
      cagccccca cagcagaat ttcgccctg agcggactt 
      caggcacct tccacaagt gaacaggga atgtataat 
      tcaccaaac agaccagtt atgggacat cccgtacct 
      aaaattgaa gggggagtt gacgcatgt tccagatct 
      agttggtta ccgcacatg catatacct catcgtggg 
      agtaaccct cccgtgtcg ccccatttg caacaaaac 
      gggttcaac aactccacc catacgaca actgctgtt 
      gttaacaac aacggtggt accataagc ggcagccaa 
      cccgcggct acggctacc atgggccaa tcgttcact 
      ggggctggt ggtacttgg acaggatct aatacgtta 
      acctacacc caatcaatg cagccccct gataaccgc 
      acccatcac acagcttac tggaacaca aaccaatgc 
      aacgacgta aacatcgct ggtctgttg tccacccag 
      ccagccccc gagtactgg tgttccgtc gcctacttc 
      gagctggac actcaagtg ggtgaaacg ttcaaagta 
      ccctcaagc tgcccgaat gtgacaatt gacggctac 
      gtcgacccc tctggaggc aaccggttt tgccttggt 
      gcgttgagt aatgtgcac aggacggac caaagcgag 
      agagcgaga ctgcacatt ggcaagggg gttcagtta 
      gatttaagg ggcgaaggc gacgtttgg ttgcgctgc 
      ttaagtgac cattccgtt ttcgtccag agttattat 
      ctggatagg gaggctgga cgacaaccc ggcgatgcc 
      gtacacaaa atttacccg tcagcctac ataaaggtc 
      tttgatcta aggcagtgt cacaaccag atgacaact 
      caggcagcc acagcacag gcagcggcg gcggcacag 
      gcggcggcg gtggcgggg cacatccct ggaccgcac 
      tcagtcggc gggatagcg ccagccata agtttgtcg 
      gcagccacg ggaataggg gttgacgat ttgcgaaga 
      ctgtgcatc ctgcgcctc agttttgtg aaaggttgg 
      ggcccggat tatcctcga cagtccatt aaagaaacg 
      ccttgttgg gttgaaatc cacctgcac cgcgctctg 
      caactgctg gacgaagtg cttcatact atgccgatt 
      gatggaccc cgtgggata gagtaa
    mRNA sequence
    • >TC010848 RA
      catgtggta ttagagttg ccgttttag cacttagca 
      tcagttgtc tcattctgt tgcagacga gaaaagaaa 
      taagaaact gagctagtg acgtaatga gtaatgtaa 
      taataatgt aaactgaaa gcaagtgtt aaacgcggg 
      ccaaaaatt aagcgccag tttgttgaa gttagtttt 
      tgcggaagc aagtgttaa ataatggtg gggctttcc 
      acgggacac atattctcc acaagttcg tcgaaagaa 
      gttggagat atgtcaggg ttagccaca acagcaccc 
      tcatcggct gacgcttgc ctgagcatt gtgcacagt 
      ctcatgtgc cacagacaa gggggagaa agcgaaggc 
      ttcgccaaa cgtgctata gagtccctg gtgaaaaag 
      ctaaaagag aaaagggat gaactagac tcgcttatc 
      actgctata accaccagt ggggcacat ccgagcaag 
      tgcgtcacg atccagagg actctcgac ggccgcctg 
      caggttgcc ggacgcaag ggctttccg cacgttatc 
      tacgctcga atctggcga tggcccgac ctccacaaa 
      aacgaactc aaacacgtg aaatattgt cagtttgcg 
      tttgattta aaatgtgac tcagtatgt gtgaatccg 
      taccattac gaacgggta gtgtccccc ggcattgac 
      ctctctgga cttacgttg caatctgga accgctcga 
      ttagtgaaa gatgagtac accgctgga ccggtccct 
      ggaggcagt atggacgta gacagcgaa atcggcatt 
      gaagtctcc cagaccatc caacaccag cccccacag 
      cagaatttc gccctgagc ggacttcag gcaccttcc 
      acaagtgaa cagggaatg tataattca ccaaacaga 
      ccagttatg ggacatccc gtacctaaa attgaaggg 
      ggagttgac gcatgttcc agatctagt tggttaccg 
      cacatgcat atacctcat cgtgggagt aaccctccc 
      gtgtcgccc catttgcaa caaaacggg ttcaacaac 
      tccacccat acgacaact gctgttgtt aacaacaac 
      ggtggtacc ataagcggc agccaaccc gcggctacg 
      gctaccatg ggccaatcg ttcactggg gctggtggt 
      acttggaca ggatctaat acgttaacc tacacccaa 
      tcaatgcag ccccctgat aaccgcacc catcacaca 
      gcttactgg aacacaaac caatgcaac gacgtaaac 
      atcgctggt ctgttgtcc acccagcca gcccccgag 
      tactggtgt tccgtcgcc tacttcgag ctggacact 
      caagtgggt gaaacgttc aaagtaccc tcaagctgc 
      ccgaatgtg acaattgac ggctacgtc gacccctct 
      ggaggcaac cggttttgc cttggtgcg ttgagtaat 
      gtgcacagg acggaccaa agcgagaga gcgagactg 
      cacattggc aagggggtt cagttagat ttaaggggc 
      gaaggcgac gtttggttg cgctgctta agtgaccat 
      tccgttttc gtccagagt tattatctg gatagggag 
      gctggacga caacccggc gatgccgta cacaaaatt 
      tacccgtca gcctacata aaggtcttt gatctaagg 
      cagtgtcac aaccagatg acaactcag gcagccaca 
      gcacaggca gcggcggcg gcacaggcg gcggcggtg 
      gcggggcac atccctgga ccgcactca gtcggcggg 
      atagcgcca gccataagt ttgtcggca gccacggga 
      ataggggtt gacgatttg cgaagactg tgcatcctg 
      cgcctcagt tttgtgaaa ggttggggc ccggattat 
      cctcgacag tccattaaa gaaacgcct tgttgggtt 
      gaaatccac ctgcaccgc gctctgcaa ctgctggac 
      gaagtgctt catactatg ccgattgat ggaccccgt 
      gggatagag taattgtgt gaatatttt gatcccctt 
      ttggtagtt cggtgggca aacaacgat tgttgtcaa 
      ttccgaatt ttatgtttg gcacgattt gacggcggc 
      aagtttgac aaaatattt tgtaagtga gtagtttat 
      aagtttgta aattacgct tatataata cttaattta 
      ataagtttg tagtgataa tcacttatt ttctacatg 
      aatatatat tttgtaaat atattgcaa acaattgcc 
      ttacagtcg gacagaact tttcatata ttttcataa 
      aattacctt ttttcaatt ggtttgtgc cattttatt 
      ttgtagaag atggatgcc aaatttgaa gtagtaatc 
      gcacacgaa atgataagg gtaaatatg tacattagg 
      ctgtgaaaa actgagtag tgtgtaagt ttttttcgt 
      ttttgttgc ttgatatta gtgtggtac tgaatgtct 
      tatttaagg aacctcttt ttgtatttt tcttgtcac 
      aagtaccta acaaactgc aattttttc atttgctgt 
      gtaaatgta gataggtat aatatagat attgtctga 
      cacaatgta tgctacttt tttgagaat tacgccatt 
      atgacatgt aattgacat tttatttgc ataatattg 
      tatttaaaa cttgtggag taaatgctt catcattcc 
      acaaaagat ctcacataa caagaaaca acatgctgt 
      taccaaaat ttttactac attcgtgat taagttttt 
      tacagttca agtaggtag taacgatgt gctgagatc 
      acagaaatc atgcgtttg attgcttat ttttgttta 
      tttataagc agttcactt gacatttaa aatagcaca 
      ggatccaat catttattt atggtttta tgtaaatac 
      ctaaaatgt aaaaataat tcttgtaaa tatacaaaa 
      ttattataa gtgtttatg aaaacatgt attgagtat 
      tataaattt ttaaccttt cactatatt ttatttgga 
      aattgttga tatatacat attttaata cattttttt 
      gactgca
    • >TC010848 RB
      catgtggta ttagagttg ccgttttag cacttagca 
      tcagttgtc tcattctgt tgcagacga gaaaagaaa 
      taagaaact gagctagtg acgtaatga gtaatgtaa 
      taataatgt aaactgaaa gcaagtgtt aaacgcggg 
      ccaaaaatt aagcgccag tttgttgaa gttagtttt 
      tgcggaagc aagtgttaa ataatggtg gggctttcc 
      acgggacac atattctcc acaagttcg tcgaaagaa 
      gcggcggtt ttcaacaac aaattaaga tctatgcaa 
      ttatttttt acaaaagtt agatttctt ccacttatt 
      tgtatcgaa aagtctact ctgcacgaa catttccgg 
      ggtttttat gcgtcccca agttggaga tatgtcagg 
      gttagccac aacagcacc ctcatcggc tgacgcttg 
      cctgagcat tgtgcacag tctcatgtg ccacagaca 
      agggggaga aagcgaagg cttcgccaa acgtgctat 
      agagtccct ggtgaaaaa gctaaaaga gaaaaggga 
      tgaactaga ctcgcttat cactgctat aaccaccag 
      tggggcaca tccgagcaa gtgcgtcac gatccagag 
      gactctcga cggccgcct gcaggttgc cggacgcaa 
      gggctttcc gcacgttat ctacgctcg aatctggcg 
      atggcccga cctccacaa aaacgaact caaacacgt 
      gaaatattg tcagtttgc gtttgattt aaaatgtga 
      ctcagtatg tgtgaatcc gtaccatta cgaacgggt 
      agtgtcccc cggcattga cctctctgg acttacgtt 
      gcaatctgg aaccgctcg attagtgaa agatgagta 
      caccgctgg accggtccc tggaggcag tatggacgt 
      agacagcga aatcggcat tgaagtctc ccagaccat 
      ccaacacca gcccccaca gcagaattt cgccctgag 
      cggacttca ggcaccttc cacaagtga acagggaat 
      gtataattc accaaacag accagttat gggacatcc 
      cgtacctaa aattgaagg gggagttga cgcatgttc 
      cagatctag ttggttacc gcacatgca tatacctca 
      tcgtgggag taaccctcc cgtgtcgcc ccatttgca 
      acaaaacgg gttcaacaa ctccaccca tacgacaac 
      tgctgttgt taacaacaa cggtggtac cataagcgg 
      cagccaacc cgcggctac ggctaccat gggccaatc 
      gttcactgg ggctggtgg tacttggac aggatctaa 
      tacgttaac ctacaccca atcaatgca gccccctga 
      taaccgcac ccatcacac agcttactg gaacacaaa 
      ccaatgcaa cgacgtaaa catcgctgg tctgttgtc 
      cacccagcc agcccccga gtactggtg ttccgtcgc 
      ctacttcga gctggacac tcaagtggg tgaaacgtt 
      caaagtacc ctcaagctg cccgaatgt gacaattga 
      cggctacgt cgacccctc tggaggcaa ccggttttg 
      ccttggtgc gttgagtaa tgtgcacag gacggacca 
      aagcgagag agcgagact gcacattgg caagggggt 
      tcagttaga tttaagggg cgaaggcga cgtttggtt 
      gcgctgctt aagtgacca ttccgtttt cgtccagag 
      ttattatct ggataggga ggctggacg acaacccgg 
      cgatgccgt acacaaaat ttacccgtc agcctacat 
      aaaggtctt tgatctaag gcagtgtca caaccagat 
      gacaactca ggcagccac agcacaggc agcggcggc 
      ggcacaggc ggcggcggt ggcggggca catccctgg 
      accgcactc agtcggcgg gatagcgcc agccataag 
      tttgtcggc agccacggg aataggggt tgacgattt 
      gcgaagact gtgcatcct gcgcctcag ttttgtgaa 
      aggttgggg cccggatta tcctcgaca gtccattaa 
      agaaacgcc ttgttgggt tgaaatcca cctgcaccg 
      cgctctgca actgctgga cgaagtgct tcatactat 
      gccgattga tggaccccg tgggataga gtaattgtg 
      tgaatattt tgatcccct tttggtagt tcggtgggc 
      aaacaacga ttgttgtca attccgaat tttatgttt 
      ggcacgatt tgacggcgg caagtttga caaaatatt 
      ttgtaagtg agtagttta taagtttgt aaattacgc 
      ttatataat acttaattt aataagttt gtagtgata 
      atcacttat tttctacat gaatatata ttttgtaaa 
      tatattgca aacaattgc cttacagtc ggacagaac 
      ttttcatat attttcata aaattacct tttttcaat 
      tggtttgtg ccattttat tttgtagaa gatggatgc 
      caaatttga agtagtaat cgcacacga aatgataag 
      ggtaaatat gtacattag gctgtgaaa aactgagta 
      gtgtgtaag tttttttcg tttttgttg cttgatatt 
      agtgtggta ctgaatgtc ttatttaag gaacctctt 
      tttgtattt ttcttgtca caagtacct aacaaactg 
      caatttttt catttgctg tgtaaatgt agataggta 
      taatataga tattgtctg acacaatgt atgctactt 
      ttttgagaa ttacgccat tatgacatg taattgaca 
      ttttatttg cataatatt gtatttaaa acttgtgga 
      gtaaatgct tcatcattc cacaaaaga tctcacata 
      acaagaaac aacatgctg ttaccaaaa tttttacta 
      cattcgtga ttaagtttt ttacagttc aagtaggta 
      gtaacgatg tgctgagat cacagaaat catgcgttt 
      gattgctta tttttgttt atttataag cagttcact 
      tgacattta aaatagcac aggatccaa tcatttatt 
      tatggtttt atgtaaata cctaaaatg taaaaataa 
      ttcttgtaa atatacaaa attattata agtgtttat 
      gaaaacatg tattgagta ttataaatt tttaacctt 
      tcactatat tttatttgg aaattgttg atatataca 
      tattttaat acatttttt tgactgca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY