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Gene information
General information
TC010845 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 5736555..5746934
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC010845
    iBeetle screen iB_04918

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010845 RA
      MRYKISVTN PSVGSNDSD TIGPGDRSF RFPLLIFLC 
VNLLLVLIA AIVLLKTPM LHNDKLCLS EQCINTASN 
ILRAMDRSA DPCTDFYQY SCGGWITTN PVPDWTATW 
DRLALLRES LMQNMKQLL ERSDEINNG NFSEIEGIR 
KARALYRIC MDTVTLESK GVAPIEHIL EVVGLSSTP 
PDNYANEDF DWLFTIART RRLLGISLL LGMNVAEDV 
RNSSANKIV IEQVSPGFG ERYLLQAEK FSTEIKNYK 
KYITQMLRA YTNFTEAEY FAEDIVNFS TQIAKVMTP 
SETRRSPNH LFHEVNLIE LANGPGIDN ESWRFVNWT 
RYLNIVFND TTVLLHPHK DTAILLDLP YLQKLSNLI 
KTSDKLVLE RFLWWSVFS TVAPLTLAK FRSLGFEFS 
QQLLGLQSR TPRWKGCTS NVNANFGLA LSYLYVKSH 
FDKNHRDKA LEMLEDIRK AFEDAVHEL DWMDTTTRE 
KTLTKLHAI RAFVGYPGW IMNATQLDK HYKQAHVVE 
GNLFETYLN LTNAAVRRN LESIRKKPD RNRWVASAT 
TVNAFYSAT LNSVTFPAG ILKPPFYGN GIEAIDYGA 
IGAIMGHEI THGFDDQGR RYDEHGNLK QWWSAATLN 
HYHQKVQCI INQYNNYTM PDLGDAFHV QGYNTQGEN 
IADNGGLRA AFEGYKRRE AVSNSMHQR LPGLPDVTT 
DQLFFLGFA QIWCGNSTL GALKSKLVD GVHSPNRIR 
VLGTLSNSR EFAKAWGCP AGTPMNPVN KCVLW
    CDS sequence
    • >TC010845 RA
      atgaggtac aaaatatca gtcacaaat cctagtgtt 
ggaagcaat gacagcgac acaattggg ccgggagat 
agatcgttc cgatttcca ctccttatt ttcttatgt 
gtcaattta ctacttgtc cttatagcg gctattgtt 
ctcttgaaa acgccaatg ctacacaac gacaagctt 
tgcctgtcc gagcaatgc atcaataca gcatcgaat 
atcctgcgt gcaatggat cggagtgcg gacccttgc 
acagatttt tatcaatat tcatgcggt ggctggatc 
accacaaat cctgttcct gactggaca gccacatgg 
gatagacta gctttactc cgggagtct ctaatgcaa 
aatatgaaa caactgctt gagcggagt gatgaaatt 
aataatggt aatttctcc gaaatagaa ggaataaga 
aaagcgcga gccctgtac agaatttgc atggataca 
gtgactctt gagtcgaaa ggggtcgcc ccaattgaa 
catatcctc gaagtggtg ggattatct tcgactccc 
cctgataat tacgctaac gaggatttt gattggcta 
ttcacaatt gcaaggaca agacgttta ctgggaata 
agtctcttg ctaggaatg aacgtggcc gaggatgtg 
cgaaactca tcggctaac aaaattgtg attgagcaa 
gtctctccc ggattcggt gagagatat ttgctgcaa 
gcagagaag ttttcaaca gaaataaag aactataaa 
aagtacatc acgcagatg cttagagcg tatacgaac 
tttacagag gctgagtat ttcgcggaa gacattgta 
aacttcagt acgcaaata gcaaaggtc atgaccccc 
tcggaaacc aggcgaagc cctaatcac ctttttcac 
gaagtcaat ctgatcgaa ctggcgaat gggccaggc 
attgacaac gagtcttgg agatttgtg aattggacg 
cgatatcta aacattgtc tttaacgac acaaccgtg 
ttgttgcat ccgcataag gacacggcg attctgctc 
gacttaccc tacttgcag aaattatca aatttaata 
aaaacctcc gataaactt gtcctggag aggttcctg 
tggtggagc gtattctca acagtagcc ccacttact 
ttagcaaag ttccgatcg ctgggattt gaattttcc 
caacaattg ttgggtctc cagagcaga actcccaga 
tggaaagga tgcaccagt aacgttaac gctaatttc 
ggcctcgcg ctaagttac ctctacgtc aagtcccat 
ttcgataag aaccaccgc gacaaggcc ttggaaatg 
ctcgaggac ataagaaaa gctttcgaa gatgcagtc 
catgaactg gattggatg gatacaacc acacgtgaa 
aagacgctg actaaactg cacgcaatc agagccttt 
gttgggtac cctggatgg attatgaat gcgacacaa 
cttgacaaa cattacaaa caagcgcac gtagtcgag 
ggcaacctc ttcgagacc tacttgaat ttaaccaac 
gcggccgtc cgcagaaac ttggaaagt attcgcaaa 
aagcctgat cgcaatcga tgggtggct tccgccacg 
accgtaaat gcattttat tcggcgaca ctgaactct 
gtcacattc ccagcagga atactaaag ccccctttt 
tacggcaac gggatcgaa gctatcgac tacggcgca 
atcggggcc atcatggga cacgaaatc acccatggg 
tttgatgac caaggccgg aggtacgac gagcacgga 
aacttgaaa cagtggtgg tccgctgcg actctcaac 
cactaccac caaaaagtc cagtgtatc ataaaccag 
tacaataat tacaccatg ccagattta ggagacgcg 
ttccacgtg caaggatac aacacgcaa ggagagaac 
atcgcggat aatggaggc cttcgcgca gcctttgaa 
ggatacaaa cgacgcgag gcggtttca aattccatg 
catcagcgg ctaccagga ctcccagat gttacaacc 
gatcaattg tttttcctc ggctttgca cagatctgg 
tgcgggaac tccactcta ggggccctt aagagcaag 
ctggtggac ggagtacac agtccgaat cgcattcgg 
gtccttggc accctttct aattcacgg gaattcgcc 
aaagcttgg ggctgtccg gcaggaacg ccgatgaat 
ccagtaaat aaatgtgta ctttggtga
    mRNA sequence
    • >TC010845 RA
      gggatttag agtaagttg ttttcaaag tgccgcagt 
ttgtttaaa ccaactaat ataattaca tcaaaatat 
gaggtacaa aatatcagt cacaaatcc tagtgttgg 
aagcaatga cagcgacac aattgggcc gggagatag 
atcgttccg atttccact ccttatttt cttatgtgt 
caatttact acttgtcct tatagcggc tattgttct 
cttgaaaac gccaatgct acacaacga caagctttg 
cctgtccga gcaatgcat caatacagc atcgaatat 
cctgcgtgc aatggatcg gagtgcgga cccttgcac 
agattttta tcaatattc atgcggtgg ctggatcac 
cacaaatcc tgttcctga ctggacagc cacatggga 
tagactagc tttactccg ggagtctct aatgcaaaa 
tatgaaaca actgcttga gcggagtga tgaaattaa 
taatggtaa tttctccga aatagaagg aataagaaa 
agcgcgagc cctgtacag aatttgcat ggatacagt 
gactcttga gtcgaaagg ggtcgcccc aattgaaca 
tatcctcga agtggtggg attatcttc gactccccc 
tgataatta cgctaacga ggattttga ttggctatt 
cacaattgc aaggacaag acgtttact gggaataag 
tctcttgct aggaatgaa cgtggccga ggatgtgcg 
aaactcatc ggctaacaa aattgtgat tgagcaagt 
ctctcccgg attcggtga gagatattt gctgcaagc 
agagaagtt ttcaacaga aataaagaa ctataaaaa 
gtacatcac gcagatgct tagagcgta tacgaactt 
tacagaggc tgagtattt cgcggaaga cattgtaaa 
cttcagtac gcaaatagc aaaggtcat gaccccctc 
ggaaaccag gcgaagccc taatcacct ttttcacga 
agtcaatct gatcgaact ggcgaatgg gccaggcat 
tgacaacga gtcttggag atttgtgaa ttggacgcg 
atatctaaa cattgtctt taacgacac aaccgtgtt 
gttgcatcc gcataagga cacggcgat tctgctcga 
cttacccta cttgcagaa attatcaaa tttaataaa 
aacctccga taaacttgt cctggagag gttcctgtg 
gtggagcgt attctcaac agtagcccc acttacttt 
agcaaagtt ccgatcgct gggatttga attttccca 
acaattgtt gggtctcca gagcagaac tcccagatg 
gaaaggatg caccagtaa cgttaacgc taatttcgg 
cctcgcgct aagttacct ctacgtcaa gtcccattt 
cgataagaa ccaccgcga caaggcctt ggaaatgct 
cgaggacat aagaaaagc tttcgaaga tgcagtcca 
tgaactgga ttggatgga tacaaccac acgtgaaaa 
gacgctgac taaactgca cgcaatcag agcctttgt 
tgggtaccc tggatggat tatgaatgc gacacaact 
tgacaaaca ttacaaaca agcgcacgt agtcgaggg 
caacctctt cgagaccta cttgaattt aaccaacgc 
ggccgtccg cagaaactt ggaaagtat tcgcaaaaa 
gcctgatcg caatcgatg ggtggcttc cgccacgac 
cgtaaatgc attttattc ggcgacact gaactctgt 
cacattccc agcaggaat actaaagcc cccttttta 
cggcaacgg gatcgaagc tatcgacta cggcgcaat 
cggggccat catgggaca cgaaatcac ccatgggtt 
tgatgacca aggccggag gtacgacga gcacggaaa 
cttgaaaca gtggtggtc cgctgcgac tctcaacca 
ctaccacca aaaagtcca gtgtatcat aaaccagta 
caataatta caccatgcc agatttagg agacgcgtt 
ccacgtgca aggatacaa cacgcaagg agagaacat 
cgcggataa tggaggcct tcgcgcagc ctttgaagg 
atacaaacg acgcgaggc ggtttcaaa ttccatgca 
tcagcggct accaggact cccagatgt tacaaccga 
tcaattgtt tttcctcgg ctttgcaca gatctggtg 
cgggaactc cactctagg ggcccttaa gagcaagct 
ggtggacgg agtacacag tccgaatcg cattcgggt 
ccttggcac cctttctaa ttcacggga attcgccaa 
agcttgggg ctgtccggc aggaacgcc gatgaatcc 
agtaaataa atgtgtact ttggtgaat cgcaacccc 
ttgattatt acattgttt acgtcaatc gcggcccga 
tcaaaacca gatttactt cattttcat aaacccgcc 
ggactaaaa ttgtttaca tccagtatt ataagatgt 
ttttaaata attttgtta ccttgtttt atttttgtt 
attacgttt tgtagaatg ccaaattgt gatataact 
tgtaggcta agataagtg taactataa ctacgtgaa 
ataaacgtt cccacaaaa t
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