| General information | |||
| TC010845 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 5736555..5746934 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC010845 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04918 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC010845 RA
MRYKISVTN PSVGSNDSD TIGPGDRSF RFPLLIFLC VNLLLVLIA AIVLLKTPM LHNDKLCLS EQCINTASN ILRAMDRSA DPCTDFYQY SCGGWITTN PVPDWTATW DRLALLRES LMQNMKQLL ERSDEINNG NFSEIEGIR KARALYRIC MDTVTLESK GVAPIEHIL EVVGLSSTP PDNYANEDF DWLFTIART RRLLGISLL LGMNVAEDV RNSSANKIV IEQVSPGFG ERYLLQAEK FSTEIKNYK KYITQMLRA YTNFTEAEY FAEDIVNFS TQIAKVMTP SETRRSPNH LFHEVNLIE LANGPGIDN ESWRFVNWT RYLNIVFND TTVLLHPHK DTAILLDLP YLQKLSNLI KTSDKLVLE RFLWWSVFS TVAPLTLAK FRSLGFEFS QQLLGLQSR TPRWKGCTS NVNANFGLA LSYLYVKSH FDKNHRDKA LEMLEDIRK AFEDAVHEL DWMDTTTRE KTLTKLHAI RAFVGYPGW IMNATQLDK HYKQAHVVE GNLFETYLN LTNAAVRRN LESIRKKPD RNRWVASAT TVNAFYSAT LNSVTFPAG ILKPPFYGN GIEAIDYGA IGAIMGHEI THGFDDQGR RYDEHGNLK QWWSAATLN HYHQKVQCI INQYNNYTM PDLGDAFHV QGYNTQGEN IADNGGLRA AFEGYKRRE AVSNSMHQR LPGLPDVTT DQLFFLGFA QIWCGNSTL GALKSKLVD GVHSPNRIR VLGTLSNSR EFAKAWGCP AGTPMNPVN KCVLW
- >TC010845 RA
atgaggtac aaaatatca gtcacaaat cctagtgtt ggaagcaat gacagcgac acaattggg ccgggagat agatcgttc cgatttcca ctccttatt ttcttatgt gtcaattta ctacttgtc cttatagcg gctattgtt ctcttgaaa acgccaatg ctacacaac gacaagctt tgcctgtcc gagcaatgc atcaataca gcatcgaat atcctgcgt gcaatggat cggagtgcg gacccttgc acagatttt tatcaatat tcatgcggt ggctggatc accacaaat cctgttcct gactggaca gccacatgg gatagacta gctttactc cgggagtct ctaatgcaa aatatgaaa caactgctt gagcggagt gatgaaatt aataatggt aatttctcc gaaatagaa ggaataaga aaagcgcga gccctgtac agaatttgc atggataca gtgactctt gagtcgaaa ggggtcgcc ccaattgaa catatcctc gaagtggtg ggattatct tcgactccc cctgataat tacgctaac gaggatttt gattggcta ttcacaatt gcaaggaca agacgttta ctgggaata agtctcttg ctaggaatg aacgtggcc gaggatgtg cgaaactca tcggctaac aaaattgtg attgagcaa gtctctccc ggattcggt gagagatat ttgctgcaa gcagagaag ttttcaaca gaaataaag aactataaa aagtacatc acgcagatg cttagagcg tatacgaac tttacagag gctgagtat ttcgcggaa gacattgta aacttcagt acgcaaata gcaaaggtc atgaccccc tcggaaacc aggcgaagc cctaatcac ctttttcac gaagtcaat ctgatcgaa ctggcgaat gggccaggc attgacaac gagtcttgg agatttgtg aattggacg cgatatcta aacattgtc tttaacgac acaaccgtg ttgttgcat ccgcataag gacacggcg attctgctc gacttaccc tacttgcag aaattatca aatttaata aaaacctcc gataaactt gtcctggag aggttcctg tggtggagc gtattctca acagtagcc ccacttact ttagcaaag ttccgatcg ctgggattt gaattttcc caacaattg ttgggtctc cagagcaga actcccaga tggaaagga tgcaccagt aacgttaac gctaatttc ggcctcgcg ctaagttac ctctacgtc aagtcccat ttcgataag aaccaccgc gacaaggcc ttggaaatg ctcgaggac ataagaaaa gctttcgaa gatgcagtc catgaactg gattggatg gatacaacc acacgtgaa aagacgctg actaaactg cacgcaatc agagccttt gttgggtac cctggatgg attatgaat gcgacacaa cttgacaaa cattacaaa caagcgcac gtagtcgag ggcaacctc ttcgagacc tacttgaat ttaaccaac gcggccgtc cgcagaaac ttggaaagt attcgcaaa aagcctgat cgcaatcga tgggtggct tccgccacg accgtaaat gcattttat tcggcgaca ctgaactct gtcacattc ccagcagga atactaaag ccccctttt tacggcaac gggatcgaa gctatcgac tacggcgca atcggggcc atcatggga cacgaaatc acccatggg tttgatgac caaggccgg aggtacgac gagcacgga aacttgaaa cagtggtgg tccgctgcg actctcaac cactaccac caaaaagtc cagtgtatc ataaaccag tacaataat tacaccatg ccagattta ggagacgcg ttccacgtg caaggatac aacacgcaa ggagagaac atcgcggat aatggaggc cttcgcgca gcctttgaa ggatacaaa cgacgcgag gcggtttca aattccatg catcagcgg ctaccagga ctcccagat gttacaacc gatcaattg tttttcctc ggctttgca cagatctgg tgcgggaac tccactcta ggggccctt aagagcaag ctggtggac ggagtacac agtccgaat cgcattcgg gtccttggc accctttct aattcacgg gaattcgcc aaagcttgg ggctgtccg gcaggaacg ccgatgaat ccagtaaat aaatgtgta ctttggtga
- >TC010845 RA
gggatttag agtaagttg ttttcaaag tgccgcagt ttgtttaaa ccaactaat ataattaca tcaaaatat gaggtacaa aatatcagt cacaaatcc tagtgttgg aagcaatga cagcgacac aattgggcc gggagatag atcgttccg atttccact ccttatttt cttatgtgt caatttact acttgtcct tatagcggc tattgttct cttgaaaac gccaatgct acacaacga caagctttg cctgtccga gcaatgcat caatacagc atcgaatat cctgcgtgc aatggatcg gagtgcgga cccttgcac agattttta tcaatattc atgcggtgg ctggatcac cacaaatcc tgttcctga ctggacagc cacatggga tagactagc tttactccg ggagtctct aatgcaaaa tatgaaaca actgcttga gcggagtga tgaaattaa taatggtaa tttctccga aatagaagg aataagaaa agcgcgagc cctgtacag aatttgcat ggatacagt gactcttga gtcgaaagg ggtcgcccc aattgaaca tatcctcga agtggtggg attatcttc gactccccc tgataatta cgctaacga ggattttga ttggctatt cacaattgc aaggacaag acgtttact gggaataag tctcttgct aggaatgaa cgtggccga ggatgtgcg aaactcatc ggctaacaa aattgtgat tgagcaagt ctctcccgg attcggtga gagatattt gctgcaagc agagaagtt ttcaacaga aataaagaa ctataaaaa gtacatcac gcagatgct tagagcgta tacgaactt tacagaggc tgagtattt cgcggaaga cattgtaaa cttcagtac gcaaatagc aaaggtcat gaccccctc ggaaaccag gcgaagccc taatcacct ttttcacga agtcaatct gatcgaact ggcgaatgg gccaggcat tgacaacga gtcttggag atttgtgaa ttggacgcg atatctaaa cattgtctt taacgacac aaccgtgtt gttgcatcc gcataagga cacggcgat tctgctcga cttacccta cttgcagaa attatcaaa tttaataaa aacctccga taaacttgt cctggagag gttcctgtg gtggagcgt attctcaac agtagcccc acttacttt agcaaagtt ccgatcgct gggatttga attttccca acaattgtt gggtctcca gagcagaac tcccagatg gaaaggatg caccagtaa cgttaacgc taatttcgg cctcgcgct aagttacct ctacgtcaa gtcccattt cgataagaa ccaccgcga caaggcctt ggaaatgct cgaggacat aagaaaagc tttcgaaga tgcagtcca tgaactgga ttggatgga tacaaccac acgtgaaaa gacgctgac taaactgca cgcaatcag agcctttgt tgggtaccc tggatggat tatgaatgc gacacaact tgacaaaca ttacaaaca agcgcacgt agtcgaggg caacctctt cgagaccta cttgaattt aaccaacgc ggccgtccg cagaaactt ggaaagtat tcgcaaaaa gcctgatcg caatcgatg ggtggcttc cgccacgac cgtaaatgc attttattc ggcgacact gaactctgt cacattccc agcaggaat actaaagcc cccttttta cggcaacgg gatcgaagc tatcgacta cggcgcaat cggggccat catgggaca cgaaatcac ccatgggtt tgatgacca aggccggag gtacgacga gcacggaaa cttgaaaca gtggtggtc cgctgcgac tctcaacca ctaccacca aaaagtcca gtgtatcat aaaccagta caataatta caccatgcc agatttagg agacgcgtt ccacgtgca aggatacaa cacgcaagg agagaacat cgcggataa tggaggcct tcgcgcagc ctttgaagg atacaaacg acgcgaggc ggtttcaaa ttccatgca tcagcggct accaggact cccagatgt tacaaccga tcaattgtt tttcctcgg ctttgcaca gatctggtg cgggaactc cactctagg ggcccttaa gagcaagct ggtggacgg agtacacag tccgaatcg cattcgggt ccttggcac cctttctaa ttcacggga attcgccaa agcttgggg ctgtccggc aggaacgcc gatgaatcc agtaaataa atgtgtact ttggtgaat cgcaacccc ttgattatt acattgttt acgtcaatc gcggcccga tcaaaacca gatttactt cattttcat aaacccgcc ggactaaaa ttgtttaca tccagtatt ataagatgt ttttaaata attttgtta ccttgtttt atttttgtt attacgttt tgtagaatg ccaaattgt gatataact tgtaggcta agataagtg taactataa ctacgtgaa ataaacgtt cccacaaaa t
