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Gene information
General information
TC010845 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 5736555..5746934
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010845
    iBeetle screen iB_04918

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010845 RA
      MRYKISVTN PSVGSNDSD TIGPGDRSF RFPLLIFLC 
      VNLLLVLIA AIVLLKTPM LHNDKLCLS EQCINTASN 
      ILRAMDRSA DPCTDFYQY SCGGWITTN PVPDWTATW 
      DRLALLRES LMQNMKQLL ERSDEINNG NFSEIEGIR 
      KARALYRIC MDTVTLESK GVAPIEHIL EVVGLSSTP 
      PDNYANEDF DWLFTIART RRLLGISLL LGMNVAEDV 
      RNSSANKIV IEQVSPGFG ERYLLQAEK FSTEIKNYK 
      KYITQMLRA YTNFTEAEY FAEDIVNFS TQIAKVMTP 
      SETRRSPNH LFHEVNLIE LANGPGIDN ESWRFVNWT 
      RYLNIVFND TTVLLHPHK DTAILLDLP YLQKLSNLI 
      KTSDKLVLE RFLWWSVFS TVAPLTLAK FRSLGFEFS 
      QQLLGLQSR TPRWKGCTS NVNANFGLA LSYLYVKSH 
      FDKNHRDKA LEMLEDIRK AFEDAVHEL DWMDTTTRE 
      KTLTKLHAI RAFVGYPGW IMNATQLDK HYKQAHVVE 
      GNLFETYLN LTNAAVRRN LESIRKKPD RNRWVASAT 
      TVNAFYSAT LNSVTFPAG ILKPPFYGN GIEAIDYGA 
      IGAIMGHEI THGFDDQGR RYDEHGNLK QWWSAATLN 
      HYHQKVQCI INQYNNYTM PDLGDAFHV QGYNTQGEN 
      IADNGGLRA AFEGYKRRE AVSNSMHQR LPGLPDVTT 
      DQLFFLGFA QIWCGNSTL GALKSKLVD GVHSPNRIR 
      VLGTLSNSR EFAKAWGCP AGTPMNPVN KCVLW
    CDS sequence
    • >TC010845 RA
      atgaggtac aaaatatca gtcacaaat cctagtgtt 
      ggaagcaat gacagcgac acaattggg ccgggagat 
      agatcgttc cgatttcca ctccttatt ttcttatgt 
      gtcaattta ctacttgtc cttatagcg gctattgtt 
      ctcttgaaa acgccaatg ctacacaac gacaagctt 
      tgcctgtcc gagcaatgc atcaataca gcatcgaat 
      atcctgcgt gcaatggat cggagtgcg gacccttgc 
      acagatttt tatcaatat tcatgcggt ggctggatc 
      accacaaat cctgttcct gactggaca gccacatgg 
      gatagacta gctttactc cgggagtct ctaatgcaa 
      aatatgaaa caactgctt gagcggagt gatgaaatt 
      aataatggt aatttctcc gaaatagaa ggaataaga 
      aaagcgcga gccctgtac agaatttgc atggataca 
      gtgactctt gagtcgaaa ggggtcgcc ccaattgaa 
      catatcctc gaagtggtg ggattatct tcgactccc 
      cctgataat tacgctaac gaggatttt gattggcta 
      ttcacaatt gcaaggaca agacgttta ctgggaata 
      agtctcttg ctaggaatg aacgtggcc gaggatgtg 
      cgaaactca tcggctaac aaaattgtg attgagcaa 
      gtctctccc ggattcggt gagagatat ttgctgcaa 
      gcagagaag ttttcaaca gaaataaag aactataaa 
      aagtacatc acgcagatg cttagagcg tatacgaac 
      tttacagag gctgagtat ttcgcggaa gacattgta 
      aacttcagt acgcaaata gcaaaggtc atgaccccc 
      tcggaaacc aggcgaagc cctaatcac ctttttcac 
      gaagtcaat ctgatcgaa ctggcgaat gggccaggc 
      attgacaac gagtcttgg agatttgtg aattggacg 
      cgatatcta aacattgtc tttaacgac acaaccgtg 
      ttgttgcat ccgcataag gacacggcg attctgctc 
      gacttaccc tacttgcag aaattatca aatttaata 
      aaaacctcc gataaactt gtcctggag aggttcctg 
      tggtggagc gtattctca acagtagcc ccacttact 
      ttagcaaag ttccgatcg ctgggattt gaattttcc 
      caacaattg ttgggtctc cagagcaga actcccaga 
      tggaaagga tgcaccagt aacgttaac gctaatttc 
      ggcctcgcg ctaagttac ctctacgtc aagtcccat 
      ttcgataag aaccaccgc gacaaggcc ttggaaatg 
      ctcgaggac ataagaaaa gctttcgaa gatgcagtc 
      catgaactg gattggatg gatacaacc acacgtgaa 
      aagacgctg actaaactg cacgcaatc agagccttt 
      gttgggtac cctggatgg attatgaat gcgacacaa 
      cttgacaaa cattacaaa caagcgcac gtagtcgag 
      ggcaacctc ttcgagacc tacttgaat ttaaccaac 
      gcggccgtc cgcagaaac ttggaaagt attcgcaaa 
      aagcctgat cgcaatcga tgggtggct tccgccacg 
      accgtaaat gcattttat tcggcgaca ctgaactct 
      gtcacattc ccagcagga atactaaag ccccctttt 
      tacggcaac gggatcgaa gctatcgac tacggcgca 
      atcggggcc atcatggga cacgaaatc acccatggg 
      tttgatgac caaggccgg aggtacgac gagcacgga 
      aacttgaaa cagtggtgg tccgctgcg actctcaac 
      cactaccac caaaaagtc cagtgtatc ataaaccag 
      tacaataat tacaccatg ccagattta ggagacgcg 
      ttccacgtg caaggatac aacacgcaa ggagagaac 
      atcgcggat aatggaggc cttcgcgca gcctttgaa 
      ggatacaaa cgacgcgag gcggtttca aattccatg 
      catcagcgg ctaccagga ctcccagat gttacaacc 
      gatcaattg tttttcctc ggctttgca cagatctgg 
      tgcgggaac tccactcta ggggccctt aagagcaag 
      ctggtggac ggagtacac agtccgaat cgcattcgg 
      gtccttggc accctttct aattcacgg gaattcgcc 
      aaagcttgg ggctgtccg gcaggaacg ccgatgaat 
      ccagtaaat aaatgtgta ctttggtga
    mRNA sequence
    • >TC010845 RA
      gggatttag agtaagttg ttttcaaag tgccgcagt 
      ttgtttaaa ccaactaat ataattaca tcaaaatat 
      gaggtacaa aatatcagt cacaaatcc tagtgttgg 
      aagcaatga cagcgacac aattgggcc gggagatag 
      atcgttccg atttccact ccttatttt cttatgtgt 
      caatttact acttgtcct tatagcggc tattgttct 
      cttgaaaac gccaatgct acacaacga caagctttg 
      cctgtccga gcaatgcat caatacagc atcgaatat 
      cctgcgtgc aatggatcg gagtgcgga cccttgcac 
      agattttta tcaatattc atgcggtgg ctggatcac 
      cacaaatcc tgttcctga ctggacagc cacatggga 
      tagactagc tttactccg ggagtctct aatgcaaaa 
      tatgaaaca actgcttga gcggagtga tgaaattaa 
      taatggtaa tttctccga aatagaagg aataagaaa 
      agcgcgagc cctgtacag aatttgcat ggatacagt 
      gactcttga gtcgaaagg ggtcgcccc aattgaaca 
      tatcctcga agtggtggg attatcttc gactccccc 
      tgataatta cgctaacga ggattttga ttggctatt 
      cacaattgc aaggacaag acgtttact gggaataag 
      tctcttgct aggaatgaa cgtggccga ggatgtgcg 
      aaactcatc ggctaacaa aattgtgat tgagcaagt 
      ctctcccgg attcggtga gagatattt gctgcaagc 
      agagaagtt ttcaacaga aataaagaa ctataaaaa 
      gtacatcac gcagatgct tagagcgta tacgaactt 
      tacagaggc tgagtattt cgcggaaga cattgtaaa 
      cttcagtac gcaaatagc aaaggtcat gaccccctc 
      ggaaaccag gcgaagccc taatcacct ttttcacga 
      agtcaatct gatcgaact ggcgaatgg gccaggcat 
      tgacaacga gtcttggag atttgtgaa ttggacgcg 
      atatctaaa cattgtctt taacgacac aaccgtgtt 
      gttgcatcc gcataagga cacggcgat tctgctcga 
      cttacccta cttgcagaa attatcaaa tttaataaa 
      aacctccga taaacttgt cctggagag gttcctgtg 
      gtggagcgt attctcaac agtagcccc acttacttt 
      agcaaagtt ccgatcgct gggatttga attttccca 
      acaattgtt gggtctcca gagcagaac tcccagatg 
      gaaaggatg caccagtaa cgttaacgc taatttcgg 
      cctcgcgct aagttacct ctacgtcaa gtcccattt 
      cgataagaa ccaccgcga caaggcctt ggaaatgct 
      cgaggacat aagaaaagc tttcgaaga tgcagtcca 
      tgaactgga ttggatgga tacaaccac acgtgaaaa 
      gacgctgac taaactgca cgcaatcag agcctttgt 
      tgggtaccc tggatggat tatgaatgc gacacaact 
      tgacaaaca ttacaaaca agcgcacgt agtcgaggg 
      caacctctt cgagaccta cttgaattt aaccaacgc 
      ggccgtccg cagaaactt ggaaagtat tcgcaaaaa 
      gcctgatcg caatcgatg ggtggcttc cgccacgac 
      cgtaaatgc attttattc ggcgacact gaactctgt 
      cacattccc agcaggaat actaaagcc cccttttta 
      cggcaacgg gatcgaagc tatcgacta cggcgcaat 
      cggggccat catgggaca cgaaatcac ccatgggtt 
      tgatgacca aggccggag gtacgacga gcacggaaa 
      cttgaaaca gtggtggtc cgctgcgac tctcaacca 
      ctaccacca aaaagtcca gtgtatcat aaaccagta 
      caataatta caccatgcc agatttagg agacgcgtt 
      ccacgtgca aggatacaa cacgcaagg agagaacat 
      cgcggataa tggaggcct tcgcgcagc ctttgaagg 
      atacaaacg acgcgaggc ggtttcaaa ttccatgca 
      tcagcggct accaggact cccagatgt tacaaccga 
      tcaattgtt tttcctcgg ctttgcaca gatctggtg 
      cgggaactc cactctagg ggcccttaa gagcaagct 
      ggtggacgg agtacacag tccgaatcg cattcgggt 
      ccttggcac cctttctaa ttcacggga attcgccaa 
      agcttgggg ctgtccggc aggaacgcc gatgaatcc 
      agtaaataa atgtgtact ttggtgaat cgcaacccc 
      ttgattatt acattgttt acgtcaatc gcggcccga 
      tcaaaacca gatttactt cattttcat aaacccgcc 
      ggactaaaa ttgtttaca tccagtatt ataagatgt 
      ttttaaata attttgtta ccttgtttt atttttgtt 
      attacgttt tgtagaatg ccaaattgt gatataact 
      tgtaggcta agataagtg taactataa ctacgtgaa 
      ataaacgtt cccacaaaa t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY