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Gene information
General information
TC010838 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 5990048..5995012
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010838
    iBeetle screen iB_04916

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 7 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010838 RA
      MEPYNKLYL AKNLDSLDL LAKKDCGDL KHVKINAIY 
      NSLNTLYKQ SQLDVLDQE KTYILLARY LDVYSFLWN 
      TESDKKFLQ LRFGSEYEK VLLHYEKTK KSLEERYAL 
      LANDSPATP QPEETQPQV AAKPSQVFD SEFISCQQL 
      YSALQKNYK FLLVDARPV SDFDESRIN HPDCINIPD 
      EIIAAGLSA NVLGKLLPD ATREKWEMR DKYDVIVLF 
      DWNTSSDNF AVTKLNKLH LSLVKWDLN RNYRERPVI 
      LKGGYLELL QTYPTLCIN PQVYLYQKN NELDELLEL 
      DDITYPTET DNKTYVMGA PGDSLSDDT SGDQAASGE 
      REYVYTRRD ILNQLQHER NELKKIAEQ CDRETNIKH 
      IAELKQRDA EIQEEIVRL ESLLGDYDK NMILDLGDE 
      STEESSDTL KDIIDEERD RELQKARAL KPKNQGPEG 
      AKDASTEVP ELDKPQINR STKPSPRPC PMVEEVEIT 
      PGLVGLRNI RNTCYLNTI IHCFRNVPD IRDLFCKGI 
      YTKFCIRNP AAIIHETAN LVRQLWSRP VRSIDPMEF 
      YTKIGELEP TYSLGHHED CMEFLLFLL NHLCEDCRY 
      DVSLPAVLT PRQKAWYNQ FEGKNSIFI DYFYHQIRI 
      TQHCRQCDQ KTFKFEIES TFMLPVPEE DFTLDELMS 
      DYMADFIIT EYCCSKCKK PVNNQKEII YEPKIMVIV 
      LKRYRQALV NGQVLLKKI ESRAHFKKR DFRLGKSRY 
      ELNAFAMHS GNMNFGHYT AAALTSKGW YEFNDENVR 
      AININDDSV KSKACVFFY SRSDD
    CDS sequence
    • >TC010838 RA
      atggaacct tacaacaaa ttatatctt gccaaaaat 
      ttggattca ttggatctt cttgcgaag aaagactgc 
      ggtgatctc aaacatgtt aaaattaat gcaatatat 
      aactccttg aacacgctg tataagcaa agtcagcta 
      gacgtcctc gaccaagaa aagacctac atcttgctg 
      gcgagatac ttggacgtt tacagcttt ctgtggaac 
      accgagtcc gataaaaag ttcctccag ttacgattc 
      ggctcggag tatgaaaag gtcttgctg cattacgag 
      aagaccaag aagagtctc gaggagcgg tacgcctta 
      ctggccaac gacagcccc gcaacccct caacctgaa 
      gagacccag ccgcaggtt gcggccaag cctagccaa 
      gttttcgac agtgaattt atatcttgc caacaatta 
      tacagtgcc ttacagaag aactacaag ttcttgctt 
      gttgatgct cgtccagtg agcgatttc gacgagtcg 
      cgcatcaac caccccgat tgcatcaac atccctgac 
      gaaataatc gcagctggt ttgtcagct aacgttttg 
      gggaaactg ttgccggac gccacccgc gagaagtgg 
      gagatgagg gacaaatac gacgtgatc gttttgttc 
      gactggaac acgtcttct gataatttt gctgtgacg 
      aagttgaac aagttgcat ttgtctcta gtcaaatgg 
      gatttgaac cgcaactat cgggaacgt cccgtgatt 
      ctgaaaggg ggctatctc gagctgctc caaacctac 
      cccaccttg tgcataaac ccgcaggtt tatctgtac 
      cagaagaac aatgaattg gacgaactg ctcgagctt 
      gacgacatc acgtacccc actgagact gacaataag 
      acatatgtc atgggggcc cccggcgac agcttgagc 
      gacgacact tccggcgat caagctgcg tcgggggaa 
      agagaatac gtgtacaca agacgcgac atcctgaac 
      cagctacaa catgaacga aatgagctg aaaaaaatc 
      gcagagcag tgcgataga gagaccaat attaaacat 
      atcgcagaa ctgaaacaa cgtgatgcg gagattcag 
      gaagagatc gtgcgtctg gagagcttg ctcggcgat 
      tatgataag aacatgatc cttgatttg ggtgatgag 
      tcaaccgag gaaagctct gatactctg aaggatatt 
      attgacgaa gagcgggac cgggagctg cagaaagcc 
      cgagccttg aagcccaaa aatcagggg ccagaaggc 
      gccaaagac gccagtacc gaggttccg gaattggac 
      aaaccccag atcaatcgc tcgactaaa ccatcccca 
      aggccctgt ccgatggtc gaggaggtg gaaattacg 
      ccaggcttg gtgggcttg cgtaatatc cgaaatacg 
      tgttatctg aacaccatc atccactgt ttccgcaac 
      gtacctgac atcagagat ctgttctgc aaaggcatt 
      tacaccaaa ttttgtatt cgcaatccg gcggcaata 
      atccacgag accgccaat ttggtgcgc cagttgtgg 
      tccagacca gtgagatca atcgatccg atggagttt 
      tacaccaaa atcggggaa ttagagccc acttattcg 
      ctgggacat cacgaagac tgcatggag tttttgctc 
      tttttgcta aaccacttg tgcgaggat tgccgctat 
      gatgtgtct ctgccggcg gttctgacg ccgcggcag 
      aaggcttgg tacaaccag ttcgagggg aagaactcg 
      attttcatc gactacttc taccaccaa attagaata 
      acccagcac tgccgacag tgcgaccaa aaaaccttc 
      aaatttgag attgagagc acgttcatg ctgccagtc 
      cctgaggaa gacttcact ctggacgaa ctgatgagc 
      gattacatg gcggacttc atcataacg gagtattgt 
      tgctccaag tgcaagaag ccggtgaac aaccagaag 
      gagattatt tacgaaccg aagatcatg gtgattgtc 
      ctcaaaagg tacagacaa gctctcgtt aacggccaa 
      gtgctgctc aaaaaaatc gaatctcgg gcccacttc 
      aaaaaacgc gacttccgg ctgggcaag agccggtat 
      gaactgaac gcgtttgcc atgcattcg gggaacatg 
      aattttggt cactacact gctgcagct ttgacttcc 
      aaggggtgg tacgaattc aatgacgaa aacgttagg 
      gcaatcaac atcaacgac gattccgtc aaatcgaag 
      gcttgtgtt ttcttctat tcgaggagt gatgactaa
    mRNA sequence
    • >TC010838 RA
      ggcgcgtga cgtcacacc atttattcg ttaaactgt 
      tttaacctc aaagcgtaa ataaaatgt ttacttctg 
      aaaacagcg aaagtttct ttgaaaagt gtgtgtttt 
      agttgaaat ttaatctaa agattgttt tggtgttga 
      tttgtggta cagttatca ttcgtcaca cgccatgga 
      accttacaa caaattata tcttgccaa aaatttgga 
      ttcattgga tcttcttgc gaagaaaga ctgcggtga 
      tctcaaaca tgttaaaat taatgcaat atataactc 
      cttgaacac gctgtataa gcaaagtca gctagacgt 
      cctcgacca agaaaagac ctacatctt gctggcgag 
      atacttgga cgtttacag ctttctgtg gaacaccga 
      gtccgataa aaagttcct ccagttacg attcggctc 
      ggagtatga aaaggtctt gctgcatta cgagaagac 
      caagaagag tctcgagga gcggtacgc cttactggc 
      caacgacag ccccgcaac ccctcaacc tgaagagac 
      ccagccgca ggttgcggc caagcctag ccaagtttt 
      cgacagtga atttatatc ttgccaaca attatacag 
      tgccttaca gaagaacta caagttctt gcttgttga 
      tgctcgtcc agtgagcga tttcgacga gtcgcgcat 
      caaccaccc cgattgcat caacatccc tgacgaaat 
      aatcgcagc tggtttgtc agctaacgt tttggggaa 
      actgttgcc ggacgccac ccgcgagaa gtgggagat 
      gagggacaa atacgacgt gatcgtttt gttcgactg 
      gaacacgtc ttctgataa ttttgctgt gacgaagtt 
      gaacaagtt gcatttgtc tctagtcaa atgggattt 
      gaaccgcaa ctatcggga acgtcccgt gattctgaa 
      agggggcta tctcgagct gctccaaac ctaccccac 
      cttgtgcat aaacccgca ggtttatct gtaccagaa 
      gaacaatga attggacga actgctcga gcttgacga 
      catcacgta ccccactga gactgacaa taagacata 
      tgtcatggg ggcccccgg cgacagctt gagcgacga 
      cacttccgg cgatcaagc tgcgtcggg ggaaagaga 
      atacgtgta cacaagacg cgacatcct gaaccagct 
      acaacatga acgaaatga gctgaaaaa aatcgcaga 
      gcagtgcga tagagagac caatattaa acatatcgc 
      agaactgaa acaacgtga tgcggagat tcaggaaga 
      gatcgtgcg tctggagag cttgctcgg cgattatga 
      taagaacat gatccttga tttgggtga tgagtcaac 
      cgaggaaag ctctgatac tctgaagga tattattga 
      cgaagagcg ggaccggga gctgcagaa agcccgagc 
      cttgaagcc caaaaatca ggggccaga aggcgccaa 
      agacgccag taccgaggt tccggaatt ggacaaacc 
      ccagatcaa tcgctcgac taaaccatc cccaaggcc 
      ctgtccgat ggtcgagga ggtggaaat tacgccagg 
      cttggtggg cttgcgtaa tatccgaaa tacgtgtta 
      tctgaacac catcatcca ctgtttccg caacgtacc 
      tgacatcag agatctgtt ctgcaaagg catttacac 
      caaattttg tattcgcaa tccggcggc aataatcca 
      cgagaccgc caatttggt gcgccagtt gtggtccag 
      accagtgag atcaatcga tccgatgga gttttacac 
      caaaatcgg ggaattaga gcccactta ttcgctggg 
      acatcacga agactgcat ggagttttt gctcttttt 
      gctaaacca cttgtgcga ggattgccg ctatgatgt 
      gtctctgcc ggcggttct gacgccgcg gcagaaggc 
      ttggtacaa ccagttcga ggggaagaa ctcgatttt 
      catcgacta cttctacca ccaaattag aataaccca 
      gcactgccg acagtgcga ccaaaaaac cttcaaatt 
      tgagattga gagcacgtt catgctgcc agtccctga 
      ggaagactt cactctgga cgaactgat gagcgatta 
      catggcgga cttcatcat aacggagta ttgttgctc 
      caagtgcaa gaagccggt gaacaacca gaaggagat 
      tatttacga accgaagat catggtgat tgtcctcaa 
      aaggtacag acaagctct cgttaacgg ccaagtgct 
      gctcaaaaa aatcgaatc tcgggccca cttcaaaaa 
      acgcgactt ccggctggg caagagccg gtatgaact 
      gaacgcgtt tgccatgca ttcggggaa catgaattt 
      tggtcacta cactgctgc agctttgac ttccaaggg 
      gtggtacga attcaatga cgaaaacgt tagggcaat 
      caacatcaa cgacgattc cgtcaaatc gaaggcttg 
      tgttttctt ctattcgag gagtgatga ctaatacgt 
      tagatttgt gatattatt aaaatgtta gcttaaaat 
      tgtacaaat gtacagttt aataagtgc tcttttcat 
      taaagttta tggaagtta tcattttga aattttaag 
      gggcttctc tgattttgt tcagtttga gaagactat 
      tgtatttaa gcgttgcta tgcattttc tatttgtat 
      ttgtgtact tgtgaagtg ccttacgga agttacccc 
      atgtttcag tattcgtta ttccatttc ttccgtagg 
      tcactattt tggtctgat gttgcatcc aagtgcaat 
      ctctttgta acagactga cagttcgtt aataaagtt 
      ttatgagtt ct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY