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Gene information
General information
TC010824 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 6094056..6097725
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010824
    iBeetle screen iB_04915

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    3 larva

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Fertility
    egg production: number no/almost no eggs -
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 4
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010824 RA
      MAVITHLKF KLFIFLCSE LTCVVLGQN RQYRPNVYG 
      SYDDRNQFS QYNNNPYQI PNPGDRDYR TYVYKGRRY 
      GQQNNFYNN RWRPGDPRI QGQDEHFFY DQQGNGEPI 
      LPGVLGGWR TDLQGKFRS QTKDKKRDI FVTTTHGQV 
      QGFLVYLFD NPDPESLYR PGSEFIERE YGMTEVYLG 
      IPYAEPPIV EARFKPPRW HKGWQLLQA VDFGPACPQ 
      PARYVGATK GIRDMDEDC LYLNVYKPP TSEEGVGKR 
      FPIMVYIHG GDFIRGASN TFPGHIMAT FYQVIVVTI 
      NYRLGALGF ISTGDVNSP GNYGILDQA MALKWIYEN 
      AESFNGDRY SITLFGPGA GAASAGLLM VAPQTKDMI 
      SKVIAQSGS ALADWTLIV DKYRAQNTS RVFGKLIGC 
      SIESSWKLM NCLKQGRSF YEIGNSEFP PEVGLFPWA 
      PVMEMNVSM PFYEGWQES DWHFLKETP EDLIRKKHF 
      NPYLKYMSG VTLQEAATF ITSNKSLEP NFIIDQEFF 
      DQKVKELVL RYNYTLNPN GTYEAIKYM YTYWPDPNN 
      KTHIRDKYI QMLSDFLYT APNDKIVKL LVEQGVEVY 
      MYVLNTTIE SFKLEEWRK VPHDIEHYL LCGAPFMDT 
      EFFPEGFSR TQWTNNDRN MSHFFMQAY TNFAKYGNP 
      TYTQILGIH FEVARHGEL KYLNLNTTY NSSIRWNFR 
      QTESAFWSQ YLPTVVGHL VPTYPPTTE YWWEPRAPL 
      QIAFWSMSA ACLLLVVLL VVCCMLWRN AKRQSDRFI 
      YDPDPDEGV DNTRLTSAY DYAEKLRTP ISVPPRTNS 
      ASTFRSESA VSLKEAQGF VSSSPSEDY PRKGTPVLS 
      HRHKSKAEL AQGVPQTDV
    CDS sequence
    • >TC010824 RA
      atggcggtt atcacccac ctgaagttc aaattgttc 
      atattcttg tgtagtgag ctaacttgt gttgttctt 
      ggacaaaac agacaatac agaccaaac gtgtacggt 
      tcgtacgat gaccgaaat cagttcagt cagtacaac 
      aacaacccg taccagatc ccgaaccca ggtgacagg 
      gattacagg acgtacgtc tacaagggg aggcgttac 
      gggcaacag aacaatttt tacaacaac aggtggcgt 
      cctggcgac ccccgaatc cagggccag gacgagcat 
      ttcttctac gaccaacaa gggaatggg gaaccgatc 
      ctgccaggg gttttgggc ggttggcgg accgacttg 
      caagggaag ttccggtcg cagacgaag gacaaaaaa 
      agggatatt ttcgtgacg accacgcac ggacaagtc 
      cagggtttt ctggtgtat ttgttcgat aatccagac 
      ccggaatcg ctctacaga cccgggtcg gagttcata 
      gaacgcgag tatggcatg actgaggtc tacttgggc 
      attccttac gccgaaccc ccgattgtc gaagcaaga 
      tttaagcct ccgaggtgg cacaaaggc tggcagttg 
      ctgcaagcg gtcgacttc ggacccgcg tgcccgcaa 
      ccagctcgc tacgtgggc gcgactaaa ggtataaga 
      gacatggac gaggactgt ctgtacctc aatgtctac 
      aagccgccg actagtgaa gagggggtt gggaagagg 
      ttcccaata atggtctac atccacggt ggcgatttt 
      atcagaggc gcttctaat acgtttcca gggcacatc 
      atggcaact ttttaccaa gtgatagtc gtgacgatt 
      aactaccgt ttaggtgcg ttgggtttt ataagcact 
      ggcgatgtc aattcaccg gggaactac ggaattttg 
      gaccaagcc atggctttg aagtggatt tatgaaaac 
      gcggagagt ttcaatggt gatagatac agtatcacg 
      ttattcgga ccaggggct ggggccgct tccgcgggc 
      ctcctcatg gtagccccc caaacgaaa gacatgatc 
      tcaaaagtc atagctcaa agcggctcc gccttagct 
      gactggacc ttgatcgtg gacaaatac cgggcccaa 
      aacactagc cgcgttttt ggcaaactc ataggctgc 
      agcattgag tcgtcctgg aagctgatg aattgtctc 
      aaacaaggg cggagtttt tacgaaata ggtaactcg 
      gagttcccg cctgaagtc ggcctattc ccttgggca 
      ccggtaatg gaaatgaac gtctcaatg cctttctat 
      gagggatgg caagagagc gattggcat ttccttaag 
      gaaactccc gaggatttg atcagaaaa aaacacttc 
      aacccttat ttgaaatat atgtcgggg gtcaccctt 
      caggaagct gcaactttt atcacttcg aataagtca 
      ctcgagccc aatttcatt atcgaccaa gagtttttc 
      gaccaaaaa gtcaaagaa ctcgtgctt cgctacaat 
      tacacgtta aatcccaac ggcacctat gaggccatc 
      aagtacatg tacacctac tggcctgat cctaacaac 
      aaaacacac attcgcgat aaatatata cagatgctc 
      tccgatttt ctctacacc gctccgaat gacaaaata 
      gtgaagctg ctggtggag caaggggtt gaagtctac 
      atgtatgtc ctgaacacc acaattgag tcgttcaaa 
      ctggaggaa tggcgaaaa gtccctcac gacattgag 
      cattatttg ctttgcggg gccccgttt atggacact 
      gagtttttc cctgagggt ttctcgcgg acccaatgg 
      acgaataac gacagaaac atgagtcat tttttcatg 
      caagcttac acgaatttc gcaaaatat ggcaaccca 
      acgtacacc caaatcctg ggcatacat ttcgaagtt 
      gccagacat ggggaactc aaatatctg aatttaaac 
      acgacgtac aattcgtca attaggtgg aatttcaga 
      cagacagag tcggcattt tggtcgcag tacttgccc 
      acagttgtg ggccatctt gtcccgacc tatccgccc 
      accactgag tattggtgg gaaccacga gcgccgctg 
      cagatcgcc ttctggagc atgtctgct gcgtgtctc 
      ctcctggtc gtgcttctt gtggtgtgc tgtatgctt 
      tggcggaac gcgaaaagg caatccgat cgttttatc 
      tacgatcct gacccggac gaaggagtt gacaatacg 
      aggcttaca agcgcgtac gattacgcc gaaaaactg 
      cgaacaccg atcagtgtt ccgccgcga acaaacagt 
      gcttccacg tttcgaagt gaaagtgcc gtgtcactg 
      aaagaagcc caaggcttc gtgagcagt agtcccagt 
      gaagattat ccgaggaaa ggcactccg gtgctgtcc 
      cacagacac aaaagcaaa gcagagttg gcgcaaggg 
      gtgccacag actgatgtt tga
    mRNA sequence
    • >TC010824 RA
      gataatatc taatgagag taggtatga gtaacaaaa 
      gtcgggcgt gtccttatt aatgagtaa ctcgcttat 
      taagtgaac aattcgtta gagtttcag tcacaagtc 
      gtgaaaagc ttctcggaa cattatgat cttgccata 
      cccaagcta aagtgacgt cactcaggt gttttgcgt 
      ccacctgta ttcagtgtt gttattccg cggtaatat 
      ggcggttat cacccacct gaagttcaa attgttcat 
      attcttgtg tagtgagct aacttgtgt tgttcttgg 
      acaaaacag acaatacag accaaacgt gtacggttc 
      gtacgatga ccgaaatca gttcagtca gtacaacaa 
      caacccgta ccagatccc gaacccagg tgacaggga 
      ttacaggac gtacgtcta caaggggag gcgttacgg 
      gcaacagaa caattttta caacaacag gtggcgtcc 
      tggcgaccc ccgaatcca gggccagga cgagcattt 
      cttctacga ccaacaagg gaatgggga accgatcct 
      gccaggggt tttgggcgg ttggcggac cgacttgca 
      agggaagtt ccggtcgca gacgaagga caaaaaaag 
      ggatatttt cgtgacgac cacgcacgg acaagtcca 
      gggttttct ggtgtattt gttcgataa tccagaccc 
      ggaatcgct ctacagacc cgggtcgga gttcataga 
      acgcgagta tggcatgac tgaggtcta cttgggcat 
      tccttacgc cgaaccccc gattgtcga agcaagatt 
      taagcctcc gaggtggca caaaggctg gcagttgct 
      gcaagcggt cgacttcgg acccgcgtg cccgcaacc 
      agctcgcta cgtgggcgc gactaaagg tataagaga 
      catggacga ggactgtct gtacctcaa tgtctacaa 
      gccgccgac tagtgaaga gggggttgg gaagaggtt 
      cccaataat ggtctacat ccacggtgg cgattttat 
      cagaggcgc ttctaatac gtttccagg gcacatcat 
      ggcaacttt ttaccaagt gatagtcgt gacgattaa 
      ctaccgttt aggtgcgtt gggttttat aagcactgg 
      cgatgtcaa ttcaccggg gaactacgg aattttgga 
      ccaagccat ggctttgaa gtggattta tgaaaacgc 
      ggagagttt caatggtga tagatacag tatcacgtt 
      attcggacc aggggctgg ggccgcttc cgcgggcct 
      cctcatggt agcccccca aacgaaaga catgatctc 
      aaaagtcat agctcaaag cggctccgc cttagctga 
      ctggacctt gatcgtgga caaataccg ggcccaaaa 
      cactagccg cgtttttgg caaactcat aggctgcag 
      cattgagtc gtcctggaa gctgatgaa ttgtctcaa 
      acaagggcg gagttttta cgaaatagg taactcgga 
      gttcccgcc tgaagtcgg cctattccc ttgggcacc 
      ggtaatgga aatgaacgt ctcaatgcc tttctatga 
      gggatggca agagagcga ttggcattt ccttaagga 
      aactcccga ggatttgat cagaaaaaa acacttcaa 
      cccttattt gaaatatat gtcgggggt cacccttca 
      ggaagctgc aacttttat cacttcgaa taagtcact 
      cgagcccaa tttcattat cgaccaaga gtttttcga 
      ccaaaaagt caaagaact cgtgcttcg ctacaatta 
      cacgttaaa tcccaacgg cacctatga ggccatcaa 
      gtacatgta cacctactg gcctgatcc taacaacaa 
      aacacacat tcgcgataa atatataca gatgctctc 
      cgattttct ctacaccgc tccgaatga caaaatagt 
      gaagctgct ggtggagca aggggttga agtctacat 
      gtatgtcct gaacaccac aattgagtc gttcaaact 
      ggaggaatg gcgaaaagt ccctcacga cattgagca 
      ttatttgct ttgcggggc cccgtttat ggacactga 
      gtttttccc tgagggttt ctcgcggac ccaatggac 
      gaataacga cagaaacat gagtcattt tttcatgca 
      agcttacac gaatttcgc aaaatatgg caacccaac 
      gtacaccca aatcctggg catacattt cgaagttgc 
      cagacatgg ggaactcaa atatctgaa tttaaacac 
      gacgtacaa ttcgtcaat taggtggaa tttcagaca 
      gacagagtc ggcattttg gtcgcagta cttgcccac 
      agttgtggg ccatcttgt cccgaccta tccgcccac 
      cactgagta ttggtggga accacgagc gccgctgca 
      gatcgcctt ctggagcat gtctgctgc gtgtctcct 
      cctggtcgt gcttcttgt ggtgtgctg tatgctttg 
      gcggaacgc gaaaaggca atccgatcg ttttatcta 
      cgatcctga cccggacga aggagttga caatacgag 
      gcttacaag cgcgtacga ttacgccga aaaactgcg 
      aacaccgat cagtgttcc gccgcgaac aaacagtgc 
      ttccacgtt tcgaagtga aagtgccgt gtcactgaa 
      agaagccca aggcttcgt gagcagtag tcccagtga 
      agattatcc gaggaaagg cactccggt gctgtccca 
      cagacacaa aagcaaagc agagttggc gcaaggggt 
      gccacagac tgatgtttg accatttgc gccatctag 
      gtccagttt catgaccac atgtgttct ttcataaaa 
      gacttatgg gtacatata gtagactaa gcttccgtc 
      catgatact gttaatgac acttaaagg tttatgtat 
      acgtatttt tatatagca aagagttaa acttaataa 
      gtacttgta aggcaatat gtttaagca aataaaaat 
      caaacattt ttacaaccg taccaaaga acaataaat 
      gaacaatga atcggaaca ggacgccac aataaaatt 
      ttgcttatt ttctaacta aaattgtag atctcgtct 
      caataaagt tttataatg ttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY