| General information | |||
| TC010811 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 5915544..5977186 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC010811 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04913 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC010811 RA
MASSTSASY ALLLWFLWF QNLPPISAQ LMWTPIYVG GNSQIDLWT QEAKGCSCP WNRTDHQCA CCVPDGGCH CGKNAPNRC TQCGLEEHC NHMCNVTII SSELMARSG KTFGQIKSP SLEGPDACW YTFIPSSTQ RIEVQIYRL VNAGKHNGT SCVGGFLQL AGGTEPQYS PSDNQICGS NERYAPPVV FFADDGLAT LLFQITEKT RRSQFLAYF SFTSINNSD GLGYQPRGG MKLQNTDCD WLYQDFACN KSEQGGDPC VLASPSYPG VYPPHRQCK YHITTSNEN IQVRISFTA LLLPQNCKT DYISIYQGS TTSSPLLTT LCGNQRKTL EYSGLNLLI EFRSGPQKP HFDYNGFVA TLKFFEKSH TTEAPTTVT QPTVRTTMS NLILPLNET VMSTPETSG CDILISGNN TRSGHFDTR EHEWHPICR LVFQGRQTD VVHVSFFNY RLRTALCKS VIEIIDNKV EDKRSIMDR ICSPVVKQA RDHDSRSVE QKTYLSTGS TMIITFKRP SAPLSTNEA EFLAGAYFF HDEQISGTM QPNGLCDVK FNGVNSPLA GMIDNPGTQ HLYWNIDGP LSCRQQFVP SSNQSITIK VQSIEKMSK EPSCVTQCG DNGCRCVSS SPLKTIDHL VLLADNNLN IACLCGSFQ SEGLPVSAR TWGSLTVIY SVAHYTWTK KGFGFSASY AFNTDTICG QHIYTIHSG EITMKNLTP PENLNHFYQ QSCTWTLHS NVERQLELD ITSNQNRPC TAWNISIHE YSPTNNQHL GDQIHTFCS RDNHKLYLL PWKLNTVVV RLFTLKIKS TLDNVDYHA DLYLF
- >TC010811 RA
atggcttcc tcaacatcg gcaagttac gcactcctc ttatggttc ttatggttc caaaatctt ccgcccatt tctgcccag ctcatgtgg acacccata tacgtcggc ggaaacagt cagatagat ttatggacg caagaggcg aagggctgc tcctgcccc tggaaccga accgaccac caatgtgct tgttgcgtg ccagatggg ggatgccat tgcggaaaa aatgccccg aatcgctgc acacaatgt ggtctagag gaacattgc aatcacatg tgtaatgtg acgataatt tcgagcgaa ttgatggca aggtccggc aaaactttc ggccagatt aagtcgccc tcgttggag ggccccgac gcctgttgg tacacattt attccatct tcaactcaa cgaatagaa gttcagatt tatagactt gtaaatgct gggaaacat aacggcacc agttgtgtg ggaggtttt ctgcaactc gccggagga acggagcca caatacagc cccagtgac aaccagatt tgtgggagc aacgaaaga tatgctccg ccagtcgtt ttcttcgct gacgatgga ttggccacg ttattattc cagataaca gagaagact aggcgttcg caattcttg gcatatttt agtttcaca tcaataaat aatagcgac gggctgggg taccaaccg cgaggagga atgaagctc caaaataca gattgcgac tggttgtat caggacttc gcttgtaac aagtctgag caaggagga gacccttgt gtattggct agtccgagt taccccgga gtgtatcca ccccatcga caatgcaaa taccacatc acaactagc aatgagaac attcaagta cgcattagt ttcactgca ctgctgtta ccgcagaat tgcaaaaca gattacata tcgatttac caaggctcg acaacaagt agtcctcta cttacaact ttatgtggt aaccaacgc aaaacttta gagtattcc ggattaaat ttactcata gagttcagg tccggaccg caaaaaccc cacttcgat tataacgga tttgtcgcc acgctgaaa tttttcgaa aaatcgcac accaccgag gccccaacc accgtcaca cagcccacc gtacggacc accatgtcc aatcttatt cttccgctg aacgaaacg gtaatgtcg actccggag acttcaggc tgtgacatt cttatatcc ggaaataac acgaggtct ggtcatttc gatacaagg gaacacgaa tggcatccc atttgccgt ttagtattc caaggccgt caaaccgac gtggtccat gtttctttc tttaattat cgattaagg actgcttta tgtaagtcc gtaatcgaa ataatagac aataaagtg gaagacaag aggagcatc atggacagg atatgtagt cccgtcgtg aaacaagct cgagatcat gacagccgt tccgtcgag caaaagaca tacttatca acgggcagc accatgata atcacattc aaaagaccc tcagcgccg ctctcaaca aacgaggcc gagtttttg gccggcgct tattttttc cacgatgaa caaattagt ggaaccatg cagcctaac ggtctctgc gacgtgaaa ttcaacgga gttaacagc cctcttgcg ggcatgata gataatccg ggcactcaa cacttatac tggaatatt gacggtcct ttatcatgc cgccaacag tttgttcct agcagcaac caaagcatc accataaaa gtccaaagt atcgagaag atgtcgaaa gagccgagc tgtgtgaca cagtgcggc gataatggc tgtcgctgt gtttcaagc agccccttg aaaactatt gatcatctc gtgctcctc gccgacaac aacctgaac atcgcctgc ctgtgcggc agcttccag agcgagggg ctgccggtg agcgcccgc acctggggc tccctcacc gtcatctac tcggtcgcc cactacact tggactaag aaggggttc ggattttcc gcgtcttat gcgttcaat accgacacg atctgcgga caacatatc tacacgatc cattcaggt gaaattacg atgaaaaac ctgacgcct ccggaaaac ctaaaccac ttctaccag caaagctgc acttggact ctgcactcc aacgtggaa cgccagcta gaactagat atcacctcc aaccaaaac cgtccttgc accgcttgg aacatcagc atccacgag tactccccg acaaacaac cagcatttg ggggaccag atccacacg ttttgctca agggacaac cacaagctc tatctgcta ccttggaag ttaaacaca gttgttgtt agattgttt acgctaaaa ataaaatct accctagac aatgtagac taccatgca gatttgtac ttattttaa
- >TC010811 RA
ccagtgttc tcagccttt tgtaaacat tccctctca tcaatgatt taccagggt tcgctttat tgaaaggaa atgtaaaac atcaaaggt tttgtatgg aaatcgcgt caaaggaaa agtgtcgat gctgtttta cgatggact tcctgtcta atgtcaaaa ttaaatcgg gattgtgaa aatggcgtt ttttgggga gtgaaggta gcgtaagat tttgagcaa ggaggaagc cactcatgg cttcctcaa catcggcaa gttacgcac tcctcttat ggttcttat ggttccaaa atcttccgc ccatttctg cccagctca tgtggacac ccatatacg tcggcggaa acagtcaga tagatttat ggacgcaag aggcgaagg gctgctcct gcccctgga accgaaccg accaccaat gtgcttgtt gcgtgccag atgggggat gccattgcg gaaaaaatg ccccgaatc gctgcacac aatgtggtc tagaggaac attgcaatc acatgtgta atgtgacga taatttcga gcgaattga tggcaaggt ccggcaaaa ctttcggcc agattaagt cgccctcgt tggagggcc ccgacgcct gttggtaca catttattc catcttcaa ctcaacgaa tagaagttc agatttata gacttgtaa atgctggga aacataacg gcaccagtt gtgtgggag gttttctgc aactcgccg gaggaacgg agccacaat acagcccca gtgacaacc agatttgtg ggagcaacg aaagatatg ctccgccag tcgttttct tcgctgacg atggattgg ccacgttat tattccaga taacagaga agactaggc gttcgcaat tcttggcat attttagtt tcacatcaa taaataata gcgacgggc tggggtacc aaccgcgag gaggaatga agctccaaa atacagatt gcgactggt tgtatcagg acttcgctt gtaacaagt ctgagcaag gaggagacc cttgtgtat tggctagtc cgagttacc ccggagtgt atccacccc atcgacaat gcaaatacc acatcacaa ctagcaatg agaacattc aagtacgca ttagtttca ctgcactgc tgttaccgc agaattgca aaacagatt acatatcga tttaccaag gctcgacaa caagtagtc ctctactta caactttat gtggtaacc aacgcaaaa ctttagagt attccggat taaatttac tcatagagt tcaggtccg gaccgcaaa aaccccact tcgattata acggatttg tcgccacgc tgaaatttt tcgaaaaat cgcacacca ccgaggccc caaccaccg tcacacagc ccaccgtac ggaccacca tgtccaatc ttattcttc cgctgaacg aaacggtaa tgtcgactc cggagactt caggctgtg acattctta tatccggaa ataacacga ggtctggtc atttcgata caagggaac acgaatggc atcccattt gccgtttag tattccaag gccgtcaaa ccgacgtgg tccatgttt ctttcttta attatcgat taaggactg ctttatgta agtccgtaa tcgaaataa tagacaata aagtggaag acaagagga gcatcatgg acaggatat gtagtcccg tcgtgaaac aagctcgag atcatgaca gccgttccg tcgagcaaa agacatact tatcaacgg gcagcacca tgataatca cattcaaaa gaccctcag cgccgctct caacaaacg aggccgagt ttttggccg gcgcttatt ttttccacg atgaacaaa ttagtggaa ccatgcagc ctaacggtc tctgcgacg tgaaattca acggagtta acagccctc ttgcgggca tgatagata atccgggca ctcaacact tatactgga atattgacg gtcctttat catgccgcc aacagtttg ttcctagca gcaaccaaa gcatcacca taaaagtcc aaagtatcg agaagatgt cgaaagagc cgagctgtg tgacacagt gcggcgata atggctgtc gctgtgttt caagcagcc ccttgaaaa ctattgatc atctcgtgc tcctcgccg acaacaacc tgaacatcg cctgcctgt gcggcagct tccagagcg aggggctgc cggtgagcg cccgcacct ggggctccc tcaccgtca tctactcgg tcgcccact acacttgga ctaagaagg ggttcggat tttccgcgt cttatgcgt tcaataccg acacgatct gcggacaac atatctaca cgatccatt caggtgaaa ttacgatga aaaacctga cgcctccgg aaaacctaa accacttct accagcaaa gctgcactt ggactctgc actccaacg tggaacgcc agctagaac tagatatca cctccaacc aaaaccgtc cttgcaccg cttggaaca tcagcatcc acgagtact ccccgacaa acaaccagc atttggggg accagatcc acacgtttt gctcaaggg acaaccaca agctctatc tgctacctt ggaagttaa acacagttg ttgttagat tgtttacgc taaaaataa aatctaccc tagacaatg tagactacc atgcagatt tgtacttat tttaattaa atatatgta tataaacga aaaatatgc ctttgaggc tgtattttc taaacatct gctcaattt gaatgccaa acactatgc atccaaaat ttaataaac tgtccacca ccttactat cccacccac ccgaatgtc aatacctgt ggtaaaagt tcctacaaa cacattttt cttcggctt atttcgtat tgaattttg gatgtgtaa atgtctatg atgtctcta aagaatgat attcattct tgacacatg tctattcat ctatctgtg atataacta tttgaaatt acgatcaca taaaatcag taaatataa agcaataat acccaaaat cggaaaaat tggatgaat aggaaaaga atataaaca atgattttc aggtcgtta actatagag taatgtaat ttaaaattc aattttgat gcattttag actctctag taactttta gttgttaga atgttatca ttatcggta gaagcttac tagagtatg aaaaaacgt acttaagga cttagtttt aatttctgt tgtatttta taaatattt acaaggagc caacttaaa ttcgcacgc tacctattg tacgacatt agcataaca aacttaaaa aggcattaa catcgcgat atatatagc ctatttttg tgccgtttt tgttacaaa ttgagcagc tgttgatgt gttaatagc actgcacaa ttttgttgt aatgttagg tttgtgtca attatgaga tatattata tgagaacac attcttcga agatgcatt tgtttgcaa taaacccaa ctagcaacc
