Status
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Gene information
General information
TC010811 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 5915544..5977186
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010811
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04913

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010811 RA
      MASSTSASY ALLLWFLWF QNLPPISAQ LMWTPIYVG 
      GNSQIDLWT QEAKGCSCP WNRTDHQCA CCVPDGGCH 
      CGKNAPNRC TQCGLEEHC NHMCNVTII SSELMARSG 
      KTFGQIKSP SLEGPDACW YTFIPSSTQ RIEVQIYRL 
      VNAGKHNGT SCVGGFLQL AGGTEPQYS PSDNQICGS 
      NERYAPPVV FFADDGLAT LLFQITEKT RRSQFLAYF 
      SFTSINNSD GLGYQPRGG MKLQNTDCD WLYQDFACN 
      KSEQGGDPC VLASPSYPG VYPPHRQCK YHITTSNEN 
      IQVRISFTA LLLPQNCKT DYISIYQGS TTSSPLLTT 
      LCGNQRKTL EYSGLNLLI EFRSGPQKP HFDYNGFVA 
      TLKFFEKSH TTEAPTTVT QPTVRTTMS NLILPLNET 
      VMSTPETSG CDILISGNN TRSGHFDTR EHEWHPICR 
      LVFQGRQTD VVHVSFFNY RLRTALCKS VIEIIDNKV 
      EDKRSIMDR ICSPVVKQA RDHDSRSVE QKTYLSTGS 
      TMIITFKRP SAPLSTNEA EFLAGAYFF HDEQISGTM 
      QPNGLCDVK FNGVNSPLA GMIDNPGTQ HLYWNIDGP 
      LSCRQQFVP SSNQSITIK VQSIEKMSK EPSCVTQCG 
      DNGCRCVSS SPLKTIDHL VLLADNNLN IACLCGSFQ 
      SEGLPVSAR TWGSLTVIY SVAHYTWTK KGFGFSASY 
      AFNTDTICG QHIYTIHSG EITMKNLTP PENLNHFYQ 
      QSCTWTLHS NVERQLELD ITSNQNRPC TAWNISIHE 
      YSPTNNQHL GDQIHTFCS RDNHKLYLL PWKLNTVVV 
      RLFTLKIKS TLDNVDYHA DLYLF
    CDS sequence
    • >TC010811 RA
      atggcttcc tcaacatcg gcaagttac gcactcctc 
      ttatggttc ttatggttc caaaatctt ccgcccatt 
      tctgcccag ctcatgtgg acacccata tacgtcggc 
      ggaaacagt cagatagat ttatggacg caagaggcg 
      aagggctgc tcctgcccc tggaaccga accgaccac 
      caatgtgct tgttgcgtg ccagatggg ggatgccat 
      tgcggaaaa aatgccccg aatcgctgc acacaatgt 
      ggtctagag gaacattgc aatcacatg tgtaatgtg 
      acgataatt tcgagcgaa ttgatggca aggtccggc 
      aaaactttc ggccagatt aagtcgccc tcgttggag 
      ggccccgac gcctgttgg tacacattt attccatct 
      tcaactcaa cgaatagaa gttcagatt tatagactt 
      gtaaatgct gggaaacat aacggcacc agttgtgtg 
      ggaggtttt ctgcaactc gccggagga acggagcca 
      caatacagc cccagtgac aaccagatt tgtgggagc 
      aacgaaaga tatgctccg ccagtcgtt ttcttcgct 
      gacgatgga ttggccacg ttattattc cagataaca 
      gagaagact aggcgttcg caattcttg gcatatttt 
      agtttcaca tcaataaat aatagcgac gggctgggg 
      taccaaccg cgaggagga atgaagctc caaaataca 
      gattgcgac tggttgtat caggacttc gcttgtaac 
      aagtctgag caaggagga gacccttgt gtattggct 
      agtccgagt taccccgga gtgtatcca ccccatcga 
      caatgcaaa taccacatc acaactagc aatgagaac 
      attcaagta cgcattagt ttcactgca ctgctgtta 
      ccgcagaat tgcaaaaca gattacata tcgatttac 
      caaggctcg acaacaagt agtcctcta cttacaact 
      ttatgtggt aaccaacgc aaaacttta gagtattcc 
      ggattaaat ttactcata gagttcagg tccggaccg 
      caaaaaccc cacttcgat tataacgga tttgtcgcc 
      acgctgaaa tttttcgaa aaatcgcac accaccgag 
      gccccaacc accgtcaca cagcccacc gtacggacc 
      accatgtcc aatcttatt cttccgctg aacgaaacg 
      gtaatgtcg actccggag acttcaggc tgtgacatt 
      cttatatcc ggaaataac acgaggtct ggtcatttc 
      gatacaagg gaacacgaa tggcatccc atttgccgt 
      ttagtattc caaggccgt caaaccgac gtggtccat 
      gtttctttc tttaattat cgattaagg actgcttta 
      tgtaagtcc gtaatcgaa ataatagac aataaagtg 
      gaagacaag aggagcatc atggacagg atatgtagt 
      cccgtcgtg aaacaagct cgagatcat gacagccgt 
      tccgtcgag caaaagaca tacttatca acgggcagc 
      accatgata atcacattc aaaagaccc tcagcgccg 
      ctctcaaca aacgaggcc gagtttttg gccggcgct 
      tattttttc cacgatgaa caaattagt ggaaccatg 
      cagcctaac ggtctctgc gacgtgaaa ttcaacgga 
      gttaacagc cctcttgcg ggcatgata gataatccg 
      ggcactcaa cacttatac tggaatatt gacggtcct 
      ttatcatgc cgccaacag tttgttcct agcagcaac 
      caaagcatc accataaaa gtccaaagt atcgagaag 
      atgtcgaaa gagccgagc tgtgtgaca cagtgcggc 
      gataatggc tgtcgctgt gtttcaagc agccccttg 
      aaaactatt gatcatctc gtgctcctc gccgacaac 
      aacctgaac atcgcctgc ctgtgcggc agcttccag 
      agcgagggg ctgccggtg agcgcccgc acctggggc 
      tccctcacc gtcatctac tcggtcgcc cactacact 
      tggactaag aaggggttc ggattttcc gcgtcttat 
      gcgttcaat accgacacg atctgcgga caacatatc 
      tacacgatc cattcaggt gaaattacg atgaaaaac 
      ctgacgcct ccggaaaac ctaaaccac ttctaccag 
      caaagctgc acttggact ctgcactcc aacgtggaa 
      cgccagcta gaactagat atcacctcc aaccaaaac 
      cgtccttgc accgcttgg aacatcagc atccacgag 
      tactccccg acaaacaac cagcatttg ggggaccag 
      atccacacg ttttgctca agggacaac cacaagctc 
      tatctgcta ccttggaag ttaaacaca gttgttgtt 
      agattgttt acgctaaaa ataaaatct accctagac 
      aatgtagac taccatgca gatttgtac ttattttaa
    mRNA sequence
    • >TC010811 RA
      ccagtgttc tcagccttt tgtaaacat tccctctca 
      tcaatgatt taccagggt tcgctttat tgaaaggaa 
      atgtaaaac atcaaaggt tttgtatgg aaatcgcgt 
      caaaggaaa agtgtcgat gctgtttta cgatggact 
      tcctgtcta atgtcaaaa ttaaatcgg gattgtgaa 
      aatggcgtt ttttgggga gtgaaggta gcgtaagat 
      tttgagcaa ggaggaagc cactcatgg cttcctcaa 
      catcggcaa gttacgcac tcctcttat ggttcttat 
      ggttccaaa atcttccgc ccatttctg cccagctca 
      tgtggacac ccatatacg tcggcggaa acagtcaga 
      tagatttat ggacgcaag aggcgaagg gctgctcct 
      gcccctgga accgaaccg accaccaat gtgcttgtt 
      gcgtgccag atgggggat gccattgcg gaaaaaatg 
      ccccgaatc gctgcacac aatgtggtc tagaggaac 
      attgcaatc acatgtgta atgtgacga taatttcga 
      gcgaattga tggcaaggt ccggcaaaa ctttcggcc 
      agattaagt cgccctcgt tggagggcc ccgacgcct 
      gttggtaca catttattc catcttcaa ctcaacgaa 
      tagaagttc agatttata gacttgtaa atgctggga 
      aacataacg gcaccagtt gtgtgggag gttttctgc 
      aactcgccg gaggaacgg agccacaat acagcccca 
      gtgacaacc agatttgtg ggagcaacg aaagatatg 
      ctccgccag tcgttttct tcgctgacg atggattgg 
      ccacgttat tattccaga taacagaga agactaggc 
      gttcgcaat tcttggcat attttagtt tcacatcaa 
      taaataata gcgacgggc tggggtacc aaccgcgag 
      gaggaatga agctccaaa atacagatt gcgactggt 
      tgtatcagg acttcgctt gtaacaagt ctgagcaag 
      gaggagacc cttgtgtat tggctagtc cgagttacc 
      ccggagtgt atccacccc atcgacaat gcaaatacc 
      acatcacaa ctagcaatg agaacattc aagtacgca 
      ttagtttca ctgcactgc tgttaccgc agaattgca 
      aaacagatt acatatcga tttaccaag gctcgacaa 
      caagtagtc ctctactta caactttat gtggtaacc 
      aacgcaaaa ctttagagt attccggat taaatttac 
      tcatagagt tcaggtccg gaccgcaaa aaccccact 
      tcgattata acggatttg tcgccacgc tgaaatttt 
      tcgaaaaat cgcacacca ccgaggccc caaccaccg 
      tcacacagc ccaccgtac ggaccacca tgtccaatc 
      ttattcttc cgctgaacg aaacggtaa tgtcgactc 
      cggagactt caggctgtg acattctta tatccggaa 
      ataacacga ggtctggtc atttcgata caagggaac 
      acgaatggc atcccattt gccgtttag tattccaag 
      gccgtcaaa ccgacgtgg tccatgttt ctttcttta 
      attatcgat taaggactg ctttatgta agtccgtaa 
      tcgaaataa tagacaata aagtggaag acaagagga 
      gcatcatgg acaggatat gtagtcccg tcgtgaaac 
      aagctcgag atcatgaca gccgttccg tcgagcaaa 
      agacatact tatcaacgg gcagcacca tgataatca 
      cattcaaaa gaccctcag cgccgctct caacaaacg 
      aggccgagt ttttggccg gcgcttatt ttttccacg 
      atgaacaaa ttagtggaa ccatgcagc ctaacggtc 
      tctgcgacg tgaaattca acggagtta acagccctc 
      ttgcgggca tgatagata atccgggca ctcaacact 
      tatactgga atattgacg gtcctttat catgccgcc 
      aacagtttg ttcctagca gcaaccaaa gcatcacca 
      taaaagtcc aaagtatcg agaagatgt cgaaagagc 
      cgagctgtg tgacacagt gcggcgata atggctgtc 
      gctgtgttt caagcagcc ccttgaaaa ctattgatc 
      atctcgtgc tcctcgccg acaacaacc tgaacatcg 
      cctgcctgt gcggcagct tccagagcg aggggctgc 
      cggtgagcg cccgcacct ggggctccc tcaccgtca 
      tctactcgg tcgcccact acacttgga ctaagaagg 
      ggttcggat tttccgcgt cttatgcgt tcaataccg 
      acacgatct gcggacaac atatctaca cgatccatt 
      caggtgaaa ttacgatga aaaacctga cgcctccgg 
      aaaacctaa accacttct accagcaaa gctgcactt 
      ggactctgc actccaacg tggaacgcc agctagaac 
      tagatatca cctccaacc aaaaccgtc cttgcaccg 
      cttggaaca tcagcatcc acgagtact ccccgacaa 
      acaaccagc atttggggg accagatcc acacgtttt 
      gctcaaggg acaaccaca agctctatc tgctacctt 
      ggaagttaa acacagttg ttgttagat tgtttacgc 
      taaaaataa aatctaccc tagacaatg tagactacc 
      atgcagatt tgtacttat tttaattaa atatatgta 
      tataaacga aaaatatgc ctttgaggc tgtattttc 
      taaacatct gctcaattt gaatgccaa acactatgc 
      atccaaaat ttaataaac tgtccacca ccttactat 
      cccacccac ccgaatgtc aatacctgt ggtaaaagt 
      tcctacaaa cacattttt cttcggctt atttcgtat 
      tgaattttg gatgtgtaa atgtctatg atgtctcta 
      aagaatgat attcattct tgacacatg tctattcat 
      ctatctgtg atataacta tttgaaatt acgatcaca 
      taaaatcag taaatataa agcaataat acccaaaat 
      cggaaaaat tggatgaat aggaaaaga atataaaca 
      atgattttc aggtcgtta actatagag taatgtaat 
      ttaaaattc aattttgat gcattttag actctctag 
      taactttta gttgttaga atgttatca ttatcggta 
      gaagcttac tagagtatg aaaaaacgt acttaagga 
      cttagtttt aatttctgt tgtatttta taaatattt 
      acaaggagc caacttaaa ttcgcacgc tacctattg 
      tacgacatt agcataaca aacttaaaa aggcattaa 
      catcgcgat atatatagc ctatttttg tgccgtttt 
      tgttacaaa ttgagcagc tgttgatgt gttaatagc 
      actgcacaa ttttgttgt aatgttagg tttgtgtca 
      attatgaga tatattata tgagaacac attcttcga 
      agatgcatt tgtttgcaa taaacccaa ctagcaacc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY