| General information | |||
| TC031532
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 5891604..5898286 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC031532 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04912 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Days post injection
Analysis of embryonic musculature and early development
number of eggs/embryos/larvae on slide: > 50
larval musculature pattern irregular -
Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 20
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
adult lethality - Number of animals showing the phenotype: 3 (19 dpi)
Days post injection
- >TC031532 RA
MLCDDNSDK VSKNPLCVF CLSPPFSIR AFTRPPERS ASRRLSVRC WRRGGSSLL SVRPRFKAV NRRSSNSLT KYLSGFFQT RARRGLTLQ EVLEEIRKA WNPQSYCTM DEKWSKSNS NETLSPVLL RRRLSAPET IMRKHMLAQ QRSDSQDSE IAQSEAKIY TYRGEMDAG SDSNLNRKR DPDLMRKTT LLRRLWSHT KPLPSTRFS GTFHEWQHS YGKLCSGRS THSLTSVHS SPEHLVKSQ SRRSLNESR TSPQHKNIN SRRLSPKHQ KRHFETDST TTTKTSTTT TPKTTTETE TSTRYTNTS YTTFSDNSD SAYTNSNSN YTETSNNNS TPSEIRKDV LEDTEASNQ GYHLRGANE TATQTIGTT NVNVISNVQ LSQSTLDLI FNQVMQDVK NSSDVSSES NIVQVKQIP SFYLKRKDS EDNSQHSRI VKCMISNVG AGSPYSKPP EAPQIAVPR FSARPQTSS MEVNISSGE STDKESDTV SLVDSLEDT SSLRTDPMQ NEVIANKSD ISLELPDNS DKKPSKPTA FFIPIEIDS RTEFKPVSD HLPQKVRDR LVRRQQKRE ERNRSKQSS SRSDSNYIS ASENGNHIH CIVHNGADI NLNSVPLPE VNYGKSKKR SKPLLPSIE SFRRIKIDD QKSVKRKSI KDENYTKSK QKLWVPKVR NKNEKLTPL YTSKNEYVY DCGPRRIYH KTEFNNTNK RIEILEIME CVDNSEKLE KLSKKSKIP VLVNQKLPK INQKQSKAE KPAFLDLDC VPITDPKLD QLIANILIE TLNIPDIQA SVSGSKSPV QESQAESAK AKFSQKFDA IPEELSFQS STENDVSNN NDVNPAEEK SAYQGKAAV AVVKDENFS SIPKGWITF YMLHNNRGS PDSTSDEGK KLADKKNES KQEIESGNN NDLTSAYSN VTYDVVKKD KYESNGNCS SSSEIPAKT LPPTTGRYK KYNQRKIRH KVSETEKVK QFGEWSVTV SGTNSYGQF APDLEMSLK FPDSQKNYT CPSQSDSGL GEDNEKSHF PKIGTKQDQ YKEEDENNE FMKQRLPDI ARTKMGKKQ RRSDSSVPS LGNLSSRIE PRQTRTSTR RYNLSRPKI DYQKSETPR NAISLFNDS HYAEILKRF PEILSITGN AINQGKK
- >TC031532 RB
MSHRCSLSL FQFFVSKNP LCVFCLSPP FSIRAFTRP PERSASRRL SVRCWRRGG SSLLSVRPR FKAVNRRSS NSLTKYLSG FFQTRARRG LTLQEVLEE IRKAWNPQS YCTMDEKWS KSNSNETLS PVLLRRRLS APETIMRKH MLAQQRSDS QDSEIAQSE AKIYTYRGE MDAGSDSNL NRKRDPDLM RKTTLLRRL WSHTKPLPS TRFSGTFHE WQHSYGKLC SGRSTHSLT SVHSSPEHL VKSQSRRSL NESRTSPQH KNINSRRLS PKHQKRHFE TDSTTTTKT STTTTPKTT TETETSTRY TNTSYTTFS DNSDSAYTN SNSNYTETS NNNSTPSEI RKDVLEDTE ASNQGYHLR GANETATQT IGTTNVNVI SNVQLSQST LDLIFNQVM QDVKNSSDV SSESNIVQV KQIPSFYLK RKDSEDNSQ HSRIVKCMI SNVGAGSPY SKPPEAPQI AVPRFSARP QTSSMEVNI SSGESTDKE SDTVSLVDS LEDTSSLRT DPMQNEVIA NKSDISLEL PDNSDKKPS KPTAFFIPI EIDSRTEFK PVSDHLPQK VRDRLVRRQ QKREERNRS KQSSSRSDS NYISASENG NHIHCIVHN GADINLNSV PLPEVNYGK SKKRSKPLL PSIESFRRI KIDDQKSVK RKSIKDENY TKSKQKLWV PKVRNKNEK LTPLYTSKN EYVYDCGPR RIYHKTEFN NTNKRIEIL EIMECVDNS EKLEKLSKK SKIPVLVNQ KLPKINQKQ SKAEKPAFL DLDCVPITD PKLDQLIAN ILIETLNIP DIQASVSGS KSPVQESQA ESAKAKFSQ KFDAIPEEL SFQSSTEND VSNNNDVNP AEEKSAYQG KAAVAVVKD ENFSSIPKG WITFYMLHN NRGSPDSTS DEGKKLADK KNESKQEIE SGNNNDLTS AYSNVTYDV VKKDKYESN GNCSSSSEI PAKTLPPTT GRYKKYNQR KIRHKVSET EKVKQFGEW SVTVSGTNS YGQFAPDLE MSLKFPDSQ KNYTCPSQS DSGLGEDNE KSHFPKIGT KQDQYKEED ENNEFMKQR LPDIARTKM GKKQRRSDS SVPSLGNLS SRIEPRQTR TSTRRYNLS RPKIDYQKS ETPRNAISL FNDSHYAEI LKRFPEILS ITGNAINQG KK
- >TC031532 RA
atgttatgt gacgacaat tctgacaag gtgtccaaa aatccgttg tgtgtgttc tgcttgtcg ccgccgttt tcaataaga gcttttaca cgtcctcct gaaaggtcg gcgtctcga cgcctttca gtccgttgc tggcgtcgc ggcggctct tcacttctc tccgtgcgt ccacgtttc aaagccgtc aatcggcgg tcctcaaac tccttaacc aaatattta tcaggattt ttccaaact cgcgccagg aggggtcta acactgcag gaggtgctc gaagagata cgcaaagct tggaatccg caaagttat tgcacgatg gatgagaaa tggagcaaa agcaactca aacgaaacg ctttcacct gtcctgctg cgaagaaga ctaagcgca ccggaaaca ataatgcga aagcacatg ttggcgcaa cagcgctcc gactcccaa gacagcgaa attgcccaa tccgaagca aaaatctac acttatcgc ggcgaaatg gacgccggg agtgattca aacttgaac cggaagcgc gatccggac ttgatgcga aaaacaacg ctgttgagg cgcctctgg agccacacc aagccgtta ccaagcacc cgattttcc gggaccttc cacgaatgg cagcactcc tacggaaaa ctgtgctcg ggaaggtcc actcactcg ctcacttcg gtgcattcg tcccccgaa cacctcgtt aaatcgcaa agcaggagg tccctcaac gagtccagg acttcgccg caacacaaa aacatcaac tcgagaaga ctttcgccg aaacaccaa aagaggcac ttcgagacg gacagcacc accaccacc aaaaccagc accaccacc acccccaaa acgacaacg gagacggaa acgagcacc cggtacacc aacacgagc tacaccacg ttctcggac aattcggac tcagcctac accaacagc aattcaaac tacacggag acttcgaac aacaactcg actccgtcc gaaattcgg aaggacgtt cttgaagac acggaagcg tcgaaccag gggtaccac ctcagaggg gccaacgaa acggcaacg caaaccatc ggcaccacc aacgttaac gtcatctca aacgtgcag ctcagccaa tcaacttta gacctaatt tttaaccaa gtgatgcaa gacgtgaaa aactctagt gacgtttcg agtgaatct aatatcgta caagtgaaa caaatcccg agtttttac ctcaaaagg aaagacagt gaggacaat tcgcaacac agtcgcata gttaaatgt atgatcagc aacgtgggg gccgggagc ccgtacagc aagcccccg gaagccccc caaatcgcc gttccgcgc ttttcggca cgaccgcaa acctcatca atggaggtt aatatatca tcaggggag tccactgat aaggaatca gacactgtc agtttagtg gacagtttg gaggacact tcgtcactg cgaacagac cccatgcag aacgaagtg atcgctaac aaaagtgac atatcgcta gagcttccc gataatagt gataagaaa cctagcaag cccactgcg ttcttcatc cccattgaa atcgattcc aggactgag tttaagcca gtttcggat catttgcct caaaaagtt agggaccgc ctagttaga agacagcaa aaacgagaa gaaagaaat aggagcaag cagagctca tctagatct gatagtaac tacattagt gcaagcgaa aacggcaat cacattcac tgtatcgtt cacaacggc gccgatatc aacttaaat agcgtacct ctccctgaa gtcaattac ggtaaaagt aaaaaacgg agcaaaccg ctcctgcct agcatcgag tcatttaga agaattaaa atcgatgac caaaaatcg gtcaagcgc aagtcaatt aaggacgaa aactacacc aaatccaag cagaaactt tgggttccg aaggtcagg aacaaaaac gagaagttg acgccgctg tacacgtcc aagaacgaa tacgtgtac gactgcggc cctcgcaga atctaccac aaaacggag tttaacaac acgaataag agaatcgaa attttggag atcatggag tgcgtcgat aactcggaa aagctggag aagttatcg aaaaagtcg aaaattccg gtcctggtc aaccagaag ttgccgaaa ataaaccaa aagcagtcg aaagccgaa aaaccggct tttttagac ttggactgc gttcccatc actgatccc aaattagac caactcatc gcaaatata ttaatagaa actttaaat attcctgat atacaggcg tcagtgagt ggcagcaaa tcgccggtg caggaatcc caggctgag tcagcgaag gcgaagttt agtcaaaaa tttgacgcg atccccgag gaactgtcg ttccagtcg tcgaccgaa aacgacgtt tcgaacaac aatgacgtc aatcccgcc gaagaaaag agcgcgtat cagggcaaa gcggccgtg gccgttgtt aaggacgaa aacttttcc tcgattcct aaaggttgg atcacgttt tacatgctg cataacaat cggggatcc cctgatagt acttccgat gaagggaaa aaactggct gacaagaaa aatgaaagc aagcaagaa attgagtcg gggaataac aatgacctg acttcggcc tacagcaac gtcacttat gacgttgtc aaaaaagac aaatacgaa tcgaatgga aactgctcc tccagcagt gaaattccg gcgaaaacg ctgcccccg accaccggt cgatataaa aagtataac cagaggaaa ataaggcat aaagtcagc gaaacggaa aaagttaag cagttcggc gaatggtcc gtgacagtg agcgggaca aacagctac ggccagttt gctcccgac ttggagatg agtctaaaa ttccccgat tctcagaaa aactacacc tgtccaagc cagtccgac tctggttta ggagaagac aatgaaaag tctcatttt ccaaaaatc ggaacaaaa caagaccag tataaagaa gaagatgaa aataatgag tttatgaag cagcgattg ccagatatt gccagaaca aaaatgggg aaaaaacag cggagaagc gattccagc gttccctcc ctcgggaac ttgtcttcc agaatcgaa ccgagacaa actcggacg agtacaaga agatataat ttgagtcga cctaaaatt gattaccaa aaatcggag acaccgaga aatgccata agcctattt aatgatagc cactatgcc gaaattttg aaacgattt ccggaaatt ttaagcatt actgggaac gccatcaac caggggaaa aaatag
- >TC031532 RB
atgtcccac agatgttcc ctatcttta ttccaattc tttgtgtcc aaaaatccg ttgtgtgtg ttctgcttg tcgccgccg ttttcaata agagctttt acacgtcct cctgaaagg tcggcgtct cgacgcctt tcagtccgt tgctggcgt cgcggcggc tcttcactt ctctccgtg cgtccacgt ttcaaagcc gtcaatcgg cggtcctca aactcctta accaaatat ttatcagga tttttccaa actcgcgcc aggaggggt ctaacactg caggaggtg ctcgaagag atacgcaaa gcttggaat ccgcaaagt tattgcacg atggatgag aaatggagc aaaagcaac tcaaacgaa acgctttca cctgtcctg ctgcgaaga agactaagc gcaccggaa acaataatg cgaaagcac atgttggcg caacagcgc tccgactcc caagacagc gaaattgcc caatccgaa gcaaaaatc tacacttat cgcggcgaa atggacgcc gggagtgat tcaaacttg aaccggaag cgcgatccg gacttgatg cgaaaaaca acgctgttg aggcgcctc tggagccac accaagccg ttaccaagc acccgattt tccgggacc ttccacgaa tggcagcac tcctacgga aaactgtgc tcgggaagg tccactcac tcgctcact tcggtgcat tcgtccccc gaacacctc gttaaatcg caaagcagg aggtccctc aacgagtcc aggacttcg ccgcaacac aaaaacatc aactcgaga agactttcg ccgaaacac caaaagagg cacttcgag acggacagc accaccacc accaaaacc agcaccacc accaccccc aaaacgaca acggagacg gaaacgagc acccggtac accaacacg agctacacc acgttctcg gacaattcg gactcagcc tacaccaac agcaattca aactacacg gagacttcg aacaacaac tcgactccg tccgaaatt cggaaggac gttcttgaa gacacggaa gcgtcgaac caggggtac cacctcaga ggggccaac gaaacggca acgcaaacc atcggcacc accaacgtt aacgtcatc tcaaacgtg cagctcagc caatcaact ttagaccta atttttaac caagtgatg caagacgtg aaaaactct agtgacgtt tcgagtgaa tctaatatc gtacaagtg aaacaaatc ccgagtttt tacctcaaa aggaaagac agtgaggac aattcgcaa cacagtcgc atagttaaa tgtatgatc agcaacgtg ggggccggg agcccgtac agcaagccc ccggaagcc ccccaaatc gccgttccg cgcttttcg gcacgaccg caaacctca tcaatggag gttaatata tcatcaggg gagtccact gataaggaa tcagacact gtcagttta gtggacagt ttggaggac acttcgtca ctgcgaaca gaccccatg cagaacgaa gtgatcgct aacaaaagt gacatatcg ctagagctt cccgataat agtgataag aaacctagc aagcccact gcgttcttc atccccatt gaaatcgat tccaggact gagtttaag ccagtttcg gatcatttg cctcaaaaa gttagggac cgcctagtt agaagacag caaaaacga gaagaaaga aataggagc aagcagagc tcatctaga tctgatagt aactacatt agtgcaagc gaaaacggc aatcacatt cactgtatc gttcacaac ggcgccgat atcaactta aatagcgta cctctccct gaagtcaat tacggtaaa agtaaaaaa cggagcaaa ccgctcctg cctagcatc gagtcattt agaagaatt aaaatcgat gaccaaaaa tcggtcaag cgcaagtca attaaggac gaaaactac accaaatcc aagcagaaa ctttgggtt ccgaaggtc aggaacaaa aacgagaag ttgacgccg ctgtacacg tccaagaac gaatacgtg tacgactgc ggccctcgc agaatctac cacaaaacg gagtttaac aacacgaat aagagaatc gaaattttg gagatcatg gagtgcgtc gataactcg gaaaagctg gagaagtta tcgaaaaag tcgaaaatt ccggtcctg gtcaaccag aagttgccg aaaataaac caaaagcag tcgaaagcc gaaaaaccg gctttttta gacttggac tgcgttccc atcactgat cccaaatta gaccaactc atcgcaaat atattaata gaaacttta aatattcct gatatacag gcgtcagtg agtggcagc aaatcgccg gtgcaggaa tcccaggct gagtcagcg aaggcgaag tttagtcaa aaatttgac gcgatcccc gaggaactg tcgttccag tcgtcgacc gaaaacgac gtttcgaac aacaatgac gtcaatccc gccgaagaa aagagcgcg tatcagggc aaagcggcc gtggccgtt gttaaggac gaaaacttt tcctcgatt cctaaaggt tggatcacg ttttacatg ctgcataac aatcgggga tcccctgat agtacttcc gatgaaggg aaaaaactg gctgacaag aaaaatgaa agcaagcaa gaaattgag tcggggaat aacaatgac ctgacttcg gcctacagc aacgtcact tatgacgtt gtcaaaaaa gacaaatac gaatcgaat ggaaactgc tcctccagc agtgaaatt ccggcgaaa acgctgccc ccgaccacc ggtcgatat aaaaagtat aaccagagg aaaataagg cataaagtc agcgaaacg gaaaaagtt aagcagttc ggcgaatgg tccgtgaca gtgagcggg acaaacagc tacggccag tttgctccc gacttggag atgagtcta aaattcccc gattctcag aaaaactac acctgtcca agccagtcc gactctggt ttaggagaa gacaatgaa aagtctcat tttccaaaa atcggaaca aaacaagac cagtataaa gaagaagat gaaaataat gagtttatg aagcagcga ttgccagat attgccaga acaaaaatg gggaaaaaa cagcggaga agcgattcc agcgttccc tccctcggg aacttgtct tccagaatc gaaccgaga caaactcgg acgagtaca agaagatat aatttgagt cgacctaaa attgattac caaaaatcg gagacaccg agaaatgcc ataagccta tttaatgat agccactat gccgaaatt ttgaaacga tttccggaa attttaagc attactggg aacgccatc aaccagggg aaaaaatag
- >TC031532 RA
tatattgtg tgaaatttt aaaaacgcc ttttattac ttttattaa tttgttttt tcaaatgtg tgagtgagt tgaggtgta aaaattgca aaatgttat gtgacgaca attctgaca aggtgtcca aaaatccgt tgtgtgtgt tctgcttgt cgccgccgt tttcaataa gagctttta cacgtcctc ctgaaaggt cggcgtctc gacgccttt cagtccgtt gctggcgtc gcggcggct cttcacttc tctccgtgc gtccacgtt tcaaagccg tcaatcggc ggtcctcaa actccttaa ccaaatatt tatcaggat ttttccaaa ctcgcgcca ggaggggtc taacactgc aggaggtgc tcgaagaga tacgcaaag cttggaatc cgcaaagtt attgcacga tggatgaga aatggagca aaagcaact caaacgaaa cgctttcac ctgtcctgc tgcgaagaa gactaagcg caccggaaa caataatgc gaaagcaca tgttggcgc aacagcgct ccgactccc aagacagcg aaattgccc aatccgaag caaaaatct acacttatc gcggcgaaa tggacgccg ggagtgatt caaacttga accggaagc gcgatccgg acttgatgc gaaaaacaa cgctgttga ggcgcctct ggagccaca ccaagccgt taccaagca cccgatttt ccgggacct tccacgaat ggcagcact cctacggaa aactgtgct cgggaaggt ccactcact cgctcactt cggtgcatt cgtcccccg aacacctcg ttaaatcgc aaagcagga ggtccctca acgagtcca ggacttcgc cgcaacaca aaaacatca actcgagaa gactttcgc cgaaacacc aaaagaggc acttcgaga cggacagca ccaccacca ccaaaacca gcaccacca ccaccccca aaacgacaa cggagacgg aaacgagca cccggtaca ccaacacga gctacacca cgttctcgg acaattcgg actcagcct acaccaaca gcaattcaa actacacgg agacttcga acaacaact cgactccgt ccgaaattc ggaaggacg ttcttgaag acacggaag cgtcgaacc aggggtacc acctcagag gggccaacg aaacggcaa cgcaaacca tcggcacca ccaacgtta acgtcatct caaacgtgc agctcagcc aatcaactt tagacctaa tttttaacc aagtgatgc aagacgtga aaaactcta gtgacgttt cgagtgaat ctaatatcg tacaagtga aacaaatcc cgagttttt acctcaaaa ggaaagaca gtgaggaca attcgcaac acagtcgca tagttaaat gtatgatca gcaacgtgg gggccggga gcccgtaca gcaagcccc cggaagccc cccaaatcg ccgttccgc gcttttcgg cacgaccgc aaacctcat caatggagg ttaatatat catcagggg agtccactg ataaggaat cagacactg tcagtttag tggacagtt tggaggaca cttcgtcac tgcgaacag accccatgc agaacgaag tgatcgcta acaaaagtg acatatcgc tagagcttc ccgataata gtgataaga aacctagca agcccactg cgttcttca tccccattg aaatcgatt ccaggactg agtttaagc cagtttcgg atcatttgc ctcaaaaag ttagggacc gcctagtta gaagacagc aaaaacgag aagaaagaa ataggagca agcagagct catctagat ctgatagta actacatta gtgcaagcg aaaacggca atcacattc actgtatcg ttcacaacg gcgccgata tcaacttaa atagcgtac ctctccctg aagtcaatt acggtaaaa gtaaaaaac ggagcaaac cgctcctgc ctagcatcg agtcattta gaagaatta aaatcgatg accaaaaat cggtcaagc gcaagtcaa ttaaggacg aaaactaca ccaaatcca agcagaaac tttgggttc cgaaggtca ggaacaaaa acgagaagt tgacgccgc tgtacacgt ccaagaacg aatacgtgt acgactgcg gccctcgca gaatctacc acaaaacgg agtttaaca acacgaata agagaatcg aaattttgg agatcatgg agtgcgtcg ataactcgg aaaagctgg agaagttat cgaaaaagt cgaaaattc cggtcctgg tcaaccaga agttgccga aaataaacc aaaagcagt cgaaagccg aaaaaccgg cttttttag acttggact gcgttccca tcactgatc ccaaattag accaactca tcgcaaata tattaatag aaactttaa atattcctg atatacagg cgtcagtga gtggcagca aatcgccgg tgcaggaat cccaggctg agtcagcga aggcgaagt ttagtcaaa aatttgacg cgatccccg aggaactgt cgttccagt cgtcgaccg aaaacgacg tttcgaaca acaatgacg tcaatcccg ccgaagaaa agagcgcgt atcagggca aagcggccg tggccgttg ttaaggacg aaaactttt cctcgattc ctaaaggtt ggatcacgt tttacatgc tgcataaca atcggggat cccctgata gtacttccg atgaaggga aaaaactgg ctgacaaga aaaatgaaa gcaagcaag aaattgagt cggggaata acaatgacc tgacttcgg cctacagca acgtcactt atgacgttg tcaaaaaag acaaatacg aatcgaatg gaaactgct cctccagca gtgaaattc cggcgaaaa cgctgcccc cgaccaccg gtcgatata aaaagtata accagagga aaataaggc ataaagtca gcgaaacgg aaaaagtta agcagttcg gcgaatggt ccgtgacag tgagcggga caaacagct acggccagt ttgctcccg acttggaga tgagtctaa aattccccg attctcaga aaaactaca cctgtccaa gccagtccg actctggtt taggagaag acaatgaaa agtctcatt ttccaaaaa tcggaacaa aacaagacc agtataaag aagaagatg aaaataatg agtttatga agcagcgat tgccagata ttgccagaa caaaaatgg ggaaaaaac agcggagaa gcgattcca gcgttccct ccctcggga acttgtctt ccagaatcg aaccgagac aaactcgga cgagtacaa gaagatata atttgagtc gacctaaaa ttgattacc aaaaatcgg agacaccga gaaatgcca taagcctat ttaatgata gccactatg ccgaaattt tgaaacgat ttccggaaa ttttaagca ttactggga acgccatca accagggga aaaaatagc gataaatag gatactcgt aagaataaa atgataact gcatt
- >TC031532 RB
ttccagaaa aaattcctt ttaccaaaa atgtcccac agatgttcc ctatcttta ttccaattc tttgtgtcc aaaaatccg ttgtgtgtg ttctgcttg tcgccgccg ttttcaata agagctttt acacgtcct cctgaaagg tcggcgtct cgacgcctt tcagtccgt tgctggcgt cgcggcggc tcttcactt ctctccgtg cgtccacgt ttcaaagcc gtcaatcgg cggtcctca aactcctta accaaatat ttatcagga tttttccaa actcgcgcc aggaggggt ctaacactg caggaggtg ctcgaagag atacgcaaa gcttggaat ccgcaaagt tattgcacg atggatgag aaatggagc aaaagcaac tcaaacgaa acgctttca cctgtcctg ctgcgaaga agactaagc gcaccggaa acaataatg cgaaagcac atgttggcg caacagcgc tccgactcc caagacagc gaaattgcc caatccgaa gcaaaaatc tacacttat cgcggcgaa atggacgcc gggagtgat tcaaacttg aaccggaag cgcgatccg gacttgatg cgaaaaaca acgctgttg aggcgcctc tggagccac accaagccg ttaccaagc acccgattt tccgggacc ttccacgaa tggcagcac tcctacgga aaactgtgc tcgggaagg tccactcac tcgctcact tcggtgcat tcgtccccc gaacacctc gttaaatcg caaagcagg aggtccctc aacgagtcc aggacttcg ccgcaacac aaaaacatc aactcgaga agactttcg ccgaaacac caaaagagg cacttcgag acggacagc accaccacc accaaaacc agcaccacc accaccccc aaaacgaca acggagacg gaaacgagc acccggtac accaacacg agctacacc acgttctcg gacaattcg gactcagcc tacaccaac agcaattca aactacacg gagacttcg aacaacaac tcgactccg tccgaaatt cggaaggac gttcttgaa gacacggaa gcgtcgaac caggggtac cacctcaga ggggccaac gaaacggca acgcaaacc atcggcacc accaacgtt aacgtcatc tcaaacgtg cagctcagc caatcaact ttagaccta atttttaac caagtgatg caagacgtg aaaaactct agtgacgtt tcgagtgaa tctaatatc gtacaagtg aaacaaatc ccgagtttt tacctcaaa aggaaagac agtgaggac aattcgcaa cacagtcgc atagttaaa tgtatgatc agcaacgtg ggggccggg agcccgtac agcaagccc ccggaagcc ccccaaatc gccgttccg cgcttttcg gcacgaccg caaacctca tcaatggag gttaatata tcatcaggg gagtccact gataaggaa tcagacact gtcagttta gtggacagt ttggaggac acttcgtca ctgcgaaca gaccccatg cagaacgaa gtgatcgct aacaaaagt gacatatcg ctagagctt cccgataat agtgataag aaacctagc aagcccact gcgttcttc atccccatt gaaatcgat tccaggact gagtttaag ccagtttcg gatcatttg cctcaaaaa gttagggac cgcctagtt agaagacag caaaaacga gaagaaaga aataggagc aagcagagc tcatctaga tctgatagt aactacatt agtgcaagc gaaaacggc aatcacatt cactgtatc gttcacaac ggcgccgat atcaactta aatagcgta cctctccct gaagtcaat tacggtaaa agtaaaaaa cggagcaaa ccgctcctg cctagcatc gagtcattt agaagaatt aaaatcgat gaccaaaaa tcggtcaag cgcaagtca attaaggac gaaaactac accaaatcc aagcagaaa ctttgggtt ccgaaggtc aggaacaaa aacgagaag ttgacgccg ctgtacacg tccaagaac gaatacgtg tacgactgc ggccctcgc agaatctac cacaaaacg gagtttaac aacacgaat aagagaatc gaaattttg gagatcatg gagtgcgtc gataactcg gaaaagctg gagaagtta tcgaaaaag tcgaaaatt ccggtcctg gtcaaccag aagttgccg aaaataaac caaaagcag tcgaaagcc gaaaaaccg gctttttta gacttggac tgcgttccc atcactgat cccaaatta gaccaactc atcgcaaat atattaata gaaacttta aatattcct gatatacag gcgtcagtg agtggcagc aaatcgccg gtgcaggaa tcccaggct gagtcagcg aaggcgaag tttagtcaa aaatttgac gcgatcccc gaggaactg tcgttccag tcgtcgacc gaaaacgac gtttcgaac aacaatgac gtcaatccc gccgaagaa aagagcgcg tatcagggc aaagcggcc gtggccgtt gttaaggac gaaaacttt tcctcgatt cctaaaggt tggatcacg ttttacatg ctgcataac aatcgggga tcccctgat agtacttcc gatgaaggg aaaaaactg gctgacaag aaaaatgaa agcaagcaa gaaattgag tcggggaat aacaatgac ctgacttcg gcctacagc aacgtcact tatgacgtt gtcaaaaaa gacaaatac gaatcgaat ggaaactgc tcctccagc agtgaaatt ccggcgaaa acgctgccc ccgaccacc ggtcgatat aaaaagtat aaccagagg aaaataagg cataaagtc agcgaaacg gaaaaagtt aagcagttc ggcgaatgg tccgtgaca gtgagcggg acaaacagc tacggccag tttgctccc gacttggag atgagtcta aaattcccc gattctcag aaaaactac acctgtcca agccagtcc gactctggt ttaggagaa gacaatgaa aagtctcat tttccaaaa atcggaaca aaacaagac cagtataaa gaagaagat gaaaataat gagtttatg aagcagcga ttgccagat attgccaga acaaaaatg gggaaaaaa cagcggaga agcgattcc agcgttccc tccctcggg aacttgtct tccagaatc gaaccgaga caaactcgg acgagtaca agaagatat aatttgagt cgacctaaa attgattac caaaaatcg gagacaccg agaaatgcc ataagccta tttaatgat agccactat gccgaaatt ttgaaacga tttccggaa attttaagc attactggg aacgccatc aaccagggg aaaaaatag cgataaata ggatactcg taagaataa aatgataac tgcatt
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