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Gene information
General information
TC031532
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010806
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 5891604..5898286
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031532
    iBeetle screen iB_04912

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031532 RA
      MLCDDNSDK VSKNPLCVF CLSPPFSIR AFTRPPERS 
      ASRRLSVRC WRRGGSSLL SVRPRFKAV NRRSSNSLT 
      KYLSGFFQT RARRGLTLQ EVLEEIRKA WNPQSYCTM 
      DEKWSKSNS NETLSPVLL RRRLSAPET IMRKHMLAQ 
      QRSDSQDSE IAQSEAKIY TYRGEMDAG SDSNLNRKR 
      DPDLMRKTT LLRRLWSHT KPLPSTRFS GTFHEWQHS 
      YGKLCSGRS THSLTSVHS SPEHLVKSQ SRRSLNESR 
      TSPQHKNIN SRRLSPKHQ KRHFETDST TTTKTSTTT 
      TPKTTTETE TSTRYTNTS YTTFSDNSD SAYTNSNSN 
      YTETSNNNS TPSEIRKDV LEDTEASNQ GYHLRGANE 
      TATQTIGTT NVNVISNVQ LSQSTLDLI FNQVMQDVK 
      NSSDVSSES NIVQVKQIP SFYLKRKDS EDNSQHSRI 
      VKCMISNVG AGSPYSKPP EAPQIAVPR FSARPQTSS 
      MEVNISSGE STDKESDTV SLVDSLEDT SSLRTDPMQ 
      NEVIANKSD ISLELPDNS DKKPSKPTA FFIPIEIDS 
      RTEFKPVSD HLPQKVRDR LVRRQQKRE ERNRSKQSS 
      SRSDSNYIS ASENGNHIH CIVHNGADI NLNSVPLPE 
      VNYGKSKKR SKPLLPSIE SFRRIKIDD QKSVKRKSI 
      KDENYTKSK QKLWVPKVR NKNEKLTPL YTSKNEYVY 
      DCGPRRIYH KTEFNNTNK RIEILEIME CVDNSEKLE 
      KLSKKSKIP VLVNQKLPK INQKQSKAE KPAFLDLDC 
      VPITDPKLD QLIANILIE TLNIPDIQA SVSGSKSPV 
      QESQAESAK AKFSQKFDA IPEELSFQS STENDVSNN 
      NDVNPAEEK SAYQGKAAV AVVKDENFS SIPKGWITF 
      YMLHNNRGS PDSTSDEGK KLADKKNES KQEIESGNN 
      NDLTSAYSN VTYDVVKKD KYESNGNCS SSSEIPAKT 
      LPPTTGRYK KYNQRKIRH KVSETEKVK QFGEWSVTV 
      SGTNSYGQF APDLEMSLK FPDSQKNYT CPSQSDSGL 
      GEDNEKSHF PKIGTKQDQ YKEEDENNE FMKQRLPDI 
      ARTKMGKKQ RRSDSSVPS LGNLSSRIE PRQTRTSTR 
      RYNLSRPKI DYQKSETPR NAISLFNDS HYAEILKRF 
      PEILSITGN AINQGKK
    • >TC031532 RB
      MSHRCSLSL FQFFVSKNP LCVFCLSPP FSIRAFTRP 
      PERSASRRL SVRCWRRGG SSLLSVRPR FKAVNRRSS 
      NSLTKYLSG FFQTRARRG LTLQEVLEE IRKAWNPQS 
      YCTMDEKWS KSNSNETLS PVLLRRRLS APETIMRKH 
      MLAQQRSDS QDSEIAQSE AKIYTYRGE MDAGSDSNL 
      NRKRDPDLM RKTTLLRRL WSHTKPLPS TRFSGTFHE 
      WQHSYGKLC SGRSTHSLT SVHSSPEHL VKSQSRRSL 
      NESRTSPQH KNINSRRLS PKHQKRHFE TDSTTTTKT 
      STTTTPKTT TETETSTRY TNTSYTTFS DNSDSAYTN 
      SNSNYTETS NNNSTPSEI RKDVLEDTE ASNQGYHLR 
      GANETATQT IGTTNVNVI SNVQLSQST LDLIFNQVM 
      QDVKNSSDV SSESNIVQV KQIPSFYLK RKDSEDNSQ 
      HSRIVKCMI SNVGAGSPY SKPPEAPQI AVPRFSARP 
      QTSSMEVNI SSGESTDKE SDTVSLVDS LEDTSSLRT 
      DPMQNEVIA NKSDISLEL PDNSDKKPS KPTAFFIPI 
      EIDSRTEFK PVSDHLPQK VRDRLVRRQ QKREERNRS 
      KQSSSRSDS NYISASENG NHIHCIVHN GADINLNSV 
      PLPEVNYGK SKKRSKPLL PSIESFRRI KIDDQKSVK 
      RKSIKDENY TKSKQKLWV PKVRNKNEK LTPLYTSKN 
      EYVYDCGPR RIYHKTEFN NTNKRIEIL EIMECVDNS 
      EKLEKLSKK SKIPVLVNQ KLPKINQKQ SKAEKPAFL 
      DLDCVPITD PKLDQLIAN ILIETLNIP DIQASVSGS 
      KSPVQESQA ESAKAKFSQ KFDAIPEEL SFQSSTEND 
      VSNNNDVNP AEEKSAYQG KAAVAVVKD ENFSSIPKG 
      WITFYMLHN NRGSPDSTS DEGKKLADK KNESKQEIE 
      SGNNNDLTS AYSNVTYDV VKKDKYESN GNCSSSSEI 
      PAKTLPPTT GRYKKYNQR KIRHKVSET EKVKQFGEW 
      SVTVSGTNS YGQFAPDLE MSLKFPDSQ KNYTCPSQS 
      DSGLGEDNE KSHFPKIGT KQDQYKEED ENNEFMKQR 
      LPDIARTKM GKKQRRSDS SVPSLGNLS SRIEPRQTR 
      TSTRRYNLS RPKIDYQKS ETPRNAISL FNDSHYAEI 
      LKRFPEILS ITGNAINQG KK
    CDS sequence
    • >TC031532 RA
      atgttatgt gacgacaat tctgacaag gtgtccaaa 
      aatccgttg tgtgtgttc tgcttgtcg ccgccgttt 
      tcaataaga gcttttaca cgtcctcct gaaaggtcg 
      gcgtctcga cgcctttca gtccgttgc tggcgtcgc 
      ggcggctct tcacttctc tccgtgcgt ccacgtttc 
      aaagccgtc aatcggcgg tcctcaaac tccttaacc 
      aaatattta tcaggattt ttccaaact cgcgccagg 
      aggggtcta acactgcag gaggtgctc gaagagata 
      cgcaaagct tggaatccg caaagttat tgcacgatg 
      gatgagaaa tggagcaaa agcaactca aacgaaacg 
      ctttcacct gtcctgctg cgaagaaga ctaagcgca 
      ccggaaaca ataatgcga aagcacatg ttggcgcaa 
      cagcgctcc gactcccaa gacagcgaa attgcccaa 
      tccgaagca aaaatctac acttatcgc ggcgaaatg 
      gacgccggg agtgattca aacttgaac cggaagcgc 
      gatccggac ttgatgcga aaaacaacg ctgttgagg 
      cgcctctgg agccacacc aagccgtta ccaagcacc 
      cgattttcc gggaccttc cacgaatgg cagcactcc 
      tacggaaaa ctgtgctcg ggaaggtcc actcactcg 
      ctcacttcg gtgcattcg tcccccgaa cacctcgtt 
      aaatcgcaa agcaggagg tccctcaac gagtccagg 
      acttcgccg caacacaaa aacatcaac tcgagaaga 
      ctttcgccg aaacaccaa aagaggcac ttcgagacg 
      gacagcacc accaccacc aaaaccagc accaccacc 
      acccccaaa acgacaacg gagacggaa acgagcacc 
      cggtacacc aacacgagc tacaccacg ttctcggac 
      aattcggac tcagcctac accaacagc aattcaaac 
      tacacggag acttcgaac aacaactcg actccgtcc 
      gaaattcgg aaggacgtt cttgaagac acggaagcg 
      tcgaaccag gggtaccac ctcagaggg gccaacgaa 
      acggcaacg caaaccatc ggcaccacc aacgttaac 
      gtcatctca aacgtgcag ctcagccaa tcaacttta 
      gacctaatt tttaaccaa gtgatgcaa gacgtgaaa 
      aactctagt gacgtttcg agtgaatct aatatcgta 
      caagtgaaa caaatcccg agtttttac ctcaaaagg 
      aaagacagt gaggacaat tcgcaacac agtcgcata 
      gttaaatgt atgatcagc aacgtgggg gccgggagc 
      ccgtacagc aagcccccg gaagccccc caaatcgcc 
      gttccgcgc ttttcggca cgaccgcaa acctcatca 
      atggaggtt aatatatca tcaggggag tccactgat 
      aaggaatca gacactgtc agtttagtg gacagtttg 
      gaggacact tcgtcactg cgaacagac cccatgcag 
      aacgaagtg atcgctaac aaaagtgac atatcgcta 
      gagcttccc gataatagt gataagaaa cctagcaag 
      cccactgcg ttcttcatc cccattgaa atcgattcc 
      aggactgag tttaagcca gtttcggat catttgcct 
      caaaaagtt agggaccgc ctagttaga agacagcaa 
      aaacgagaa gaaagaaat aggagcaag cagagctca 
      tctagatct gatagtaac tacattagt gcaagcgaa 
      aacggcaat cacattcac tgtatcgtt cacaacggc 
      gccgatatc aacttaaat agcgtacct ctccctgaa 
      gtcaattac ggtaaaagt aaaaaacgg agcaaaccg 
      ctcctgcct agcatcgag tcatttaga agaattaaa 
      atcgatgac caaaaatcg gtcaagcgc aagtcaatt 
      aaggacgaa aactacacc aaatccaag cagaaactt 
      tgggttccg aaggtcagg aacaaaaac gagaagttg 
      acgccgctg tacacgtcc aagaacgaa tacgtgtac 
      gactgcggc cctcgcaga atctaccac aaaacggag 
      tttaacaac acgaataag agaatcgaa attttggag 
      atcatggag tgcgtcgat aactcggaa aagctggag 
      aagttatcg aaaaagtcg aaaattccg gtcctggtc 
      aaccagaag ttgccgaaa ataaaccaa aagcagtcg 
      aaagccgaa aaaccggct tttttagac ttggactgc 
      gttcccatc actgatccc aaattagac caactcatc 
      gcaaatata ttaatagaa actttaaat attcctgat 
      atacaggcg tcagtgagt ggcagcaaa tcgccggtg 
      caggaatcc caggctgag tcagcgaag gcgaagttt 
      agtcaaaaa tttgacgcg atccccgag gaactgtcg 
      ttccagtcg tcgaccgaa aacgacgtt tcgaacaac 
      aatgacgtc aatcccgcc gaagaaaag agcgcgtat 
      cagggcaaa gcggccgtg gccgttgtt aaggacgaa 
      aacttttcc tcgattcct aaaggttgg atcacgttt 
      tacatgctg cataacaat cggggatcc cctgatagt 
      acttccgat gaagggaaa aaactggct gacaagaaa 
      aatgaaagc aagcaagaa attgagtcg gggaataac 
      aatgacctg acttcggcc tacagcaac gtcacttat 
      gacgttgtc aaaaaagac aaatacgaa tcgaatgga 
      aactgctcc tccagcagt gaaattccg gcgaaaacg 
      ctgcccccg accaccggt cgatataaa aagtataac 
      cagaggaaa ataaggcat aaagtcagc gaaacggaa 
      aaagttaag cagttcggc gaatggtcc gtgacagtg 
      agcgggaca aacagctac ggccagttt gctcccgac 
      ttggagatg agtctaaaa ttccccgat tctcagaaa 
      aactacacc tgtccaagc cagtccgac tctggttta 
      ggagaagac aatgaaaag tctcatttt ccaaaaatc 
      ggaacaaaa caagaccag tataaagaa gaagatgaa 
      aataatgag tttatgaag cagcgattg ccagatatt 
      gccagaaca aaaatgggg aaaaaacag cggagaagc 
      gattccagc gttccctcc ctcgggaac ttgtcttcc 
      agaatcgaa ccgagacaa actcggacg agtacaaga 
      agatataat ttgagtcga cctaaaatt gattaccaa 
      aaatcggag acaccgaga aatgccata agcctattt 
      aatgatagc cactatgcc gaaattttg aaacgattt 
      ccggaaatt ttaagcatt actgggaac gccatcaac 
      caggggaaa aaatag
    • >TC031532 RB
      atgtcccac agatgttcc ctatcttta ttccaattc 
      tttgtgtcc aaaaatccg ttgtgtgtg ttctgcttg 
      tcgccgccg ttttcaata agagctttt acacgtcct 
      cctgaaagg tcggcgtct cgacgcctt tcagtccgt 
      tgctggcgt cgcggcggc tcttcactt ctctccgtg 
      cgtccacgt ttcaaagcc gtcaatcgg cggtcctca 
      aactcctta accaaatat ttatcagga tttttccaa 
      actcgcgcc aggaggggt ctaacactg caggaggtg 
      ctcgaagag atacgcaaa gcttggaat ccgcaaagt 
      tattgcacg atggatgag aaatggagc aaaagcaac 
      tcaaacgaa acgctttca cctgtcctg ctgcgaaga 
      agactaagc gcaccggaa acaataatg cgaaagcac 
      atgttggcg caacagcgc tccgactcc caagacagc 
      gaaattgcc caatccgaa gcaaaaatc tacacttat 
      cgcggcgaa atggacgcc gggagtgat tcaaacttg 
      aaccggaag cgcgatccg gacttgatg cgaaaaaca 
      acgctgttg aggcgcctc tggagccac accaagccg 
      ttaccaagc acccgattt tccgggacc ttccacgaa 
      tggcagcac tcctacgga aaactgtgc tcgggaagg 
      tccactcac tcgctcact tcggtgcat tcgtccccc 
      gaacacctc gttaaatcg caaagcagg aggtccctc 
      aacgagtcc aggacttcg ccgcaacac aaaaacatc 
      aactcgaga agactttcg ccgaaacac caaaagagg 
      cacttcgag acggacagc accaccacc accaaaacc 
      agcaccacc accaccccc aaaacgaca acggagacg 
      gaaacgagc acccggtac accaacacg agctacacc 
      acgttctcg gacaattcg gactcagcc tacaccaac 
      agcaattca aactacacg gagacttcg aacaacaac 
      tcgactccg tccgaaatt cggaaggac gttcttgaa 
      gacacggaa gcgtcgaac caggggtac cacctcaga 
      ggggccaac gaaacggca acgcaaacc atcggcacc 
      accaacgtt aacgtcatc tcaaacgtg cagctcagc 
      caatcaact ttagaccta atttttaac caagtgatg 
      caagacgtg aaaaactct agtgacgtt tcgagtgaa 
      tctaatatc gtacaagtg aaacaaatc ccgagtttt 
      tacctcaaa aggaaagac agtgaggac aattcgcaa 
      cacagtcgc atagttaaa tgtatgatc agcaacgtg 
      ggggccggg agcccgtac agcaagccc ccggaagcc 
      ccccaaatc gccgttccg cgcttttcg gcacgaccg 
      caaacctca tcaatggag gttaatata tcatcaggg 
      gagtccact gataaggaa tcagacact gtcagttta 
      gtggacagt ttggaggac acttcgtca ctgcgaaca 
      gaccccatg cagaacgaa gtgatcgct aacaaaagt 
      gacatatcg ctagagctt cccgataat agtgataag 
      aaacctagc aagcccact gcgttcttc atccccatt 
      gaaatcgat tccaggact gagtttaag ccagtttcg 
      gatcatttg cctcaaaaa gttagggac cgcctagtt 
      agaagacag caaaaacga gaagaaaga aataggagc 
      aagcagagc tcatctaga tctgatagt aactacatt 
      agtgcaagc gaaaacggc aatcacatt cactgtatc 
      gttcacaac ggcgccgat atcaactta aatagcgta 
      cctctccct gaagtcaat tacggtaaa agtaaaaaa 
      cggagcaaa ccgctcctg cctagcatc gagtcattt 
      agaagaatt aaaatcgat gaccaaaaa tcggtcaag 
      cgcaagtca attaaggac gaaaactac accaaatcc 
      aagcagaaa ctttgggtt ccgaaggtc aggaacaaa 
      aacgagaag ttgacgccg ctgtacacg tccaagaac 
      gaatacgtg tacgactgc ggccctcgc agaatctac 
      cacaaaacg gagtttaac aacacgaat aagagaatc 
      gaaattttg gagatcatg gagtgcgtc gataactcg 
      gaaaagctg gagaagtta tcgaaaaag tcgaaaatt 
      ccggtcctg gtcaaccag aagttgccg aaaataaac 
      caaaagcag tcgaaagcc gaaaaaccg gctttttta 
      gacttggac tgcgttccc atcactgat cccaaatta 
      gaccaactc atcgcaaat atattaata gaaacttta 
      aatattcct gatatacag gcgtcagtg agtggcagc 
      aaatcgccg gtgcaggaa tcccaggct gagtcagcg 
      aaggcgaag tttagtcaa aaatttgac gcgatcccc 
      gaggaactg tcgttccag tcgtcgacc gaaaacgac 
      gtttcgaac aacaatgac gtcaatccc gccgaagaa 
      aagagcgcg tatcagggc aaagcggcc gtggccgtt 
      gttaaggac gaaaacttt tcctcgatt cctaaaggt 
      tggatcacg ttttacatg ctgcataac aatcgggga 
      tcccctgat agtacttcc gatgaaggg aaaaaactg 
      gctgacaag aaaaatgaa agcaagcaa gaaattgag 
      tcggggaat aacaatgac ctgacttcg gcctacagc 
      aacgtcact tatgacgtt gtcaaaaaa gacaaatac 
      gaatcgaat ggaaactgc tcctccagc agtgaaatt 
      ccggcgaaa acgctgccc ccgaccacc ggtcgatat 
      aaaaagtat aaccagagg aaaataagg cataaagtc 
      agcgaaacg gaaaaagtt aagcagttc ggcgaatgg 
      tccgtgaca gtgagcggg acaaacagc tacggccag 
      tttgctccc gacttggag atgagtcta aaattcccc 
      gattctcag aaaaactac acctgtcca agccagtcc 
      gactctggt ttaggagaa gacaatgaa aagtctcat 
      tttccaaaa atcggaaca aaacaagac cagtataaa 
      gaagaagat gaaaataat gagtttatg aagcagcga 
      ttgccagat attgccaga acaaaaatg gggaaaaaa 
      cagcggaga agcgattcc agcgttccc tccctcggg 
      aacttgtct tccagaatc gaaccgaga caaactcgg 
      acgagtaca agaagatat aatttgagt cgacctaaa 
      attgattac caaaaatcg gagacaccg agaaatgcc 
      ataagccta tttaatgat agccactat gccgaaatt 
      ttgaaacga tttccggaa attttaagc attactggg 
      aacgccatc aaccagggg aaaaaatag
    mRNA sequence
    • >TC031532 RA
      tatattgtg tgaaatttt aaaaacgcc ttttattac 
      ttttattaa tttgttttt tcaaatgtg tgagtgagt 
      tgaggtgta aaaattgca aaatgttat gtgacgaca 
      attctgaca aggtgtcca aaaatccgt tgtgtgtgt 
      tctgcttgt cgccgccgt tttcaataa gagctttta 
      cacgtcctc ctgaaaggt cggcgtctc gacgccttt 
      cagtccgtt gctggcgtc gcggcggct cttcacttc 
      tctccgtgc gtccacgtt tcaaagccg tcaatcggc 
      ggtcctcaa actccttaa ccaaatatt tatcaggat 
      ttttccaaa ctcgcgcca ggaggggtc taacactgc 
      aggaggtgc tcgaagaga tacgcaaag cttggaatc 
      cgcaaagtt attgcacga tggatgaga aatggagca 
      aaagcaact caaacgaaa cgctttcac ctgtcctgc 
      tgcgaagaa gactaagcg caccggaaa caataatgc 
      gaaagcaca tgttggcgc aacagcgct ccgactccc 
      aagacagcg aaattgccc aatccgaag caaaaatct 
      acacttatc gcggcgaaa tggacgccg ggagtgatt 
      caaacttga accggaagc gcgatccgg acttgatgc 
      gaaaaacaa cgctgttga ggcgcctct ggagccaca 
      ccaagccgt taccaagca cccgatttt ccgggacct 
      tccacgaat ggcagcact cctacggaa aactgtgct 
      cgggaaggt ccactcact cgctcactt cggtgcatt 
      cgtcccccg aacacctcg ttaaatcgc aaagcagga 
      ggtccctca acgagtcca ggacttcgc cgcaacaca 
      aaaacatca actcgagaa gactttcgc cgaaacacc 
      aaaagaggc acttcgaga cggacagca ccaccacca 
      ccaaaacca gcaccacca ccaccccca aaacgacaa 
      cggagacgg aaacgagca cccggtaca ccaacacga 
      gctacacca cgttctcgg acaattcgg actcagcct 
      acaccaaca gcaattcaa actacacgg agacttcga 
      acaacaact cgactccgt ccgaaattc ggaaggacg 
      ttcttgaag acacggaag cgtcgaacc aggggtacc 
      acctcagag gggccaacg aaacggcaa cgcaaacca 
      tcggcacca ccaacgtta acgtcatct caaacgtgc 
      agctcagcc aatcaactt tagacctaa tttttaacc 
      aagtgatgc aagacgtga aaaactcta gtgacgttt 
      cgagtgaat ctaatatcg tacaagtga aacaaatcc 
      cgagttttt acctcaaaa ggaaagaca gtgaggaca 
      attcgcaac acagtcgca tagttaaat gtatgatca 
      gcaacgtgg gggccggga gcccgtaca gcaagcccc 
      cggaagccc cccaaatcg ccgttccgc gcttttcgg 
      cacgaccgc aaacctcat caatggagg ttaatatat 
      catcagggg agtccactg ataaggaat cagacactg 
      tcagtttag tggacagtt tggaggaca cttcgtcac 
      tgcgaacag accccatgc agaacgaag tgatcgcta 
      acaaaagtg acatatcgc tagagcttc ccgataata 
      gtgataaga aacctagca agcccactg cgttcttca 
      tccccattg aaatcgatt ccaggactg agtttaagc 
      cagtttcgg atcatttgc ctcaaaaag ttagggacc 
      gcctagtta gaagacagc aaaaacgag aagaaagaa 
      ataggagca agcagagct catctagat ctgatagta 
      actacatta gtgcaagcg aaaacggca atcacattc 
      actgtatcg ttcacaacg gcgccgata tcaacttaa 
      atagcgtac ctctccctg aagtcaatt acggtaaaa 
      gtaaaaaac ggagcaaac cgctcctgc ctagcatcg 
      agtcattta gaagaatta aaatcgatg accaaaaat 
      cggtcaagc gcaagtcaa ttaaggacg aaaactaca 
      ccaaatcca agcagaaac tttgggttc cgaaggtca 
      ggaacaaaa acgagaagt tgacgccgc tgtacacgt 
      ccaagaacg aatacgtgt acgactgcg gccctcgca 
      gaatctacc acaaaacgg agtttaaca acacgaata 
      agagaatcg aaattttgg agatcatgg agtgcgtcg 
      ataactcgg aaaagctgg agaagttat cgaaaaagt 
      cgaaaattc cggtcctgg tcaaccaga agttgccga 
      aaataaacc aaaagcagt cgaaagccg aaaaaccgg 
      cttttttag acttggact gcgttccca tcactgatc 
      ccaaattag accaactca tcgcaaata tattaatag 
      aaactttaa atattcctg atatacagg cgtcagtga 
      gtggcagca aatcgccgg tgcaggaat cccaggctg 
      agtcagcga aggcgaagt ttagtcaaa aatttgacg 
      cgatccccg aggaactgt cgttccagt cgtcgaccg 
      aaaacgacg tttcgaaca acaatgacg tcaatcccg 
      ccgaagaaa agagcgcgt atcagggca aagcggccg 
      tggccgttg ttaaggacg aaaactttt cctcgattc 
      ctaaaggtt ggatcacgt tttacatgc tgcataaca 
      atcggggat cccctgata gtacttccg atgaaggga 
      aaaaactgg ctgacaaga aaaatgaaa gcaagcaag 
      aaattgagt cggggaata acaatgacc tgacttcgg 
      cctacagca acgtcactt atgacgttg tcaaaaaag 
      acaaatacg aatcgaatg gaaactgct cctccagca 
      gtgaaattc cggcgaaaa cgctgcccc cgaccaccg 
      gtcgatata aaaagtata accagagga aaataaggc 
      ataaagtca gcgaaacgg aaaaagtta agcagttcg 
      gcgaatggt ccgtgacag tgagcggga caaacagct 
      acggccagt ttgctcccg acttggaga tgagtctaa 
      aattccccg attctcaga aaaactaca cctgtccaa 
      gccagtccg actctggtt taggagaag acaatgaaa 
      agtctcatt ttccaaaaa tcggaacaa aacaagacc 
      agtataaag aagaagatg aaaataatg agtttatga 
      agcagcgat tgccagata ttgccagaa caaaaatgg 
      ggaaaaaac agcggagaa gcgattcca gcgttccct 
      ccctcggga acttgtctt ccagaatcg aaccgagac 
      aaactcgga cgagtacaa gaagatata atttgagtc 
      gacctaaaa ttgattacc aaaaatcgg agacaccga 
      gaaatgcca taagcctat ttaatgata gccactatg 
      ccgaaattt tgaaacgat ttccggaaa ttttaagca 
      ttactggga acgccatca accagggga aaaaatagc 
      gataaatag gatactcgt aagaataaa atgataact 
      gcatt
    • >TC031532 RB
      ttccagaaa aaattcctt ttaccaaaa atgtcccac 
      agatgttcc ctatcttta ttccaattc tttgtgtcc 
      aaaaatccg ttgtgtgtg ttctgcttg tcgccgccg 
      ttttcaata agagctttt acacgtcct cctgaaagg 
      tcggcgtct cgacgcctt tcagtccgt tgctggcgt 
      cgcggcggc tcttcactt ctctccgtg cgtccacgt 
      ttcaaagcc gtcaatcgg cggtcctca aactcctta 
      accaaatat ttatcagga tttttccaa actcgcgcc 
      aggaggggt ctaacactg caggaggtg ctcgaagag 
      atacgcaaa gcttggaat ccgcaaagt tattgcacg 
      atggatgag aaatggagc aaaagcaac tcaaacgaa 
      acgctttca cctgtcctg ctgcgaaga agactaagc 
      gcaccggaa acaataatg cgaaagcac atgttggcg 
      caacagcgc tccgactcc caagacagc gaaattgcc 
      caatccgaa gcaaaaatc tacacttat cgcggcgaa 
      atggacgcc gggagtgat tcaaacttg aaccggaag 
      cgcgatccg gacttgatg cgaaaaaca acgctgttg 
      aggcgcctc tggagccac accaagccg ttaccaagc 
      acccgattt tccgggacc ttccacgaa tggcagcac 
      tcctacgga aaactgtgc tcgggaagg tccactcac 
      tcgctcact tcggtgcat tcgtccccc gaacacctc 
      gttaaatcg caaagcagg aggtccctc aacgagtcc 
      aggacttcg ccgcaacac aaaaacatc aactcgaga 
      agactttcg ccgaaacac caaaagagg cacttcgag 
      acggacagc accaccacc accaaaacc agcaccacc 
      accaccccc aaaacgaca acggagacg gaaacgagc 
      acccggtac accaacacg agctacacc acgttctcg 
      gacaattcg gactcagcc tacaccaac agcaattca 
      aactacacg gagacttcg aacaacaac tcgactccg 
      tccgaaatt cggaaggac gttcttgaa gacacggaa 
      gcgtcgaac caggggtac cacctcaga ggggccaac 
      gaaacggca acgcaaacc atcggcacc accaacgtt 
      aacgtcatc tcaaacgtg cagctcagc caatcaact 
      ttagaccta atttttaac caagtgatg caagacgtg 
      aaaaactct agtgacgtt tcgagtgaa tctaatatc 
      gtacaagtg aaacaaatc ccgagtttt tacctcaaa 
      aggaaagac agtgaggac aattcgcaa cacagtcgc 
      atagttaaa tgtatgatc agcaacgtg ggggccggg 
      agcccgtac agcaagccc ccggaagcc ccccaaatc 
      gccgttccg cgcttttcg gcacgaccg caaacctca 
      tcaatggag gttaatata tcatcaggg gagtccact 
      gataaggaa tcagacact gtcagttta gtggacagt 
      ttggaggac acttcgtca ctgcgaaca gaccccatg 
      cagaacgaa gtgatcgct aacaaaagt gacatatcg 
      ctagagctt cccgataat agtgataag aaacctagc 
      aagcccact gcgttcttc atccccatt gaaatcgat 
      tccaggact gagtttaag ccagtttcg gatcatttg 
      cctcaaaaa gttagggac cgcctagtt agaagacag 
      caaaaacga gaagaaaga aataggagc aagcagagc 
      tcatctaga tctgatagt aactacatt agtgcaagc 
      gaaaacggc aatcacatt cactgtatc gttcacaac 
      ggcgccgat atcaactta aatagcgta cctctccct 
      gaagtcaat tacggtaaa agtaaaaaa cggagcaaa 
      ccgctcctg cctagcatc gagtcattt agaagaatt 
      aaaatcgat gaccaaaaa tcggtcaag cgcaagtca 
      attaaggac gaaaactac accaaatcc aagcagaaa 
      ctttgggtt ccgaaggtc aggaacaaa aacgagaag 
      ttgacgccg ctgtacacg tccaagaac gaatacgtg 
      tacgactgc ggccctcgc agaatctac cacaaaacg 
      gagtttaac aacacgaat aagagaatc gaaattttg 
      gagatcatg gagtgcgtc gataactcg gaaaagctg 
      gagaagtta tcgaaaaag tcgaaaatt ccggtcctg 
      gtcaaccag aagttgccg aaaataaac caaaagcag 
      tcgaaagcc gaaaaaccg gctttttta gacttggac 
      tgcgttccc atcactgat cccaaatta gaccaactc 
      atcgcaaat atattaata gaaacttta aatattcct 
      gatatacag gcgtcagtg agtggcagc aaatcgccg 
      gtgcaggaa tcccaggct gagtcagcg aaggcgaag 
      tttagtcaa aaatttgac gcgatcccc gaggaactg 
      tcgttccag tcgtcgacc gaaaacgac gtttcgaac 
      aacaatgac gtcaatccc gccgaagaa aagagcgcg 
      tatcagggc aaagcggcc gtggccgtt gttaaggac 
      gaaaacttt tcctcgatt cctaaaggt tggatcacg 
      ttttacatg ctgcataac aatcgggga tcccctgat 
      agtacttcc gatgaaggg aaaaaactg gctgacaag 
      aaaaatgaa agcaagcaa gaaattgag tcggggaat 
      aacaatgac ctgacttcg gcctacagc aacgtcact 
      tatgacgtt gtcaaaaaa gacaaatac gaatcgaat 
      ggaaactgc tcctccagc agtgaaatt ccggcgaaa 
      acgctgccc ccgaccacc ggtcgatat aaaaagtat 
      aaccagagg aaaataagg cataaagtc agcgaaacg 
      gaaaaagtt aagcagttc ggcgaatgg tccgtgaca 
      gtgagcggg acaaacagc tacggccag tttgctccc 
      gacttggag atgagtcta aaattcccc gattctcag 
      aaaaactac acctgtcca agccagtcc gactctggt 
      ttaggagaa gacaatgaa aagtctcat tttccaaaa 
      atcggaaca aaacaagac cagtataaa gaagaagat 
      gaaaataat gagtttatg aagcagcga ttgccagat 
      attgccaga acaaaaatg gggaaaaaa cagcggaga 
      agcgattcc agcgttccc tccctcggg aacttgtct 
      tccagaatc gaaccgaga caaactcgg acgagtaca 
      agaagatat aatttgagt cgacctaaa attgattac 
      caaaaatcg gagacaccg agaaatgcc ataagccta 
      tttaatgat agccactat gccgaaatt ttgaaacga 
      tttccggaa attttaagc attactggg aacgccatc 
      aaccagggg aaaaaatag cgataaata ggatactcg 
      taagaataa aatgataac tgcatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY