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Gene information
General information
TC010803 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 5866695..5870249
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010803
    iBeetle screen iB_04911

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010803 RA
      MHCVISRFV VLLVAFTYV SSTSILDFW NWGHEGSDL 
      EQRDDNSDS TALRTCKCD GPSCMCCVD FNLTYIDLG 
      GPGCVRMRY ISQDEGIAV NVSYGESLL HSEQVKGPN 
      PAPTCMALL ANLAQVCAR FSDLQPYND GLRGCLLLE 
      PKLLGDVTA SFNIGCFTM GPEGMKLEE SVNSTLTTL 
      LQPEKGSNN ATQSESEGL NEEELIAAV NESAEQGLV 
      FFGNLLGLT FGKPETNET NTEAVAPAT NTSNETATS 
      T
    CDS sequence
    • >TC010803 RA
      atgcattgc gtgatatca cgatttgtt gtacttctt 
      gttgcattc acttatgtg tcttccacc agtatctta 
      gatttctgg aactgggga cacgaagga agtgactta 
      gaacagagg gatgacaac tcagattcg acggccctc 
      agaacttgc aaatgtgat ggaccgtct tgtatgtgc 
      tgcgtcgat ttcaatctg acctacatc gacctggga 
      gggccaggt tgtgttcga atgagatac atttcgcag 
      gacgaaggg attgctgtc aacgtttcc tatggggaa 
      agtcttctt cacagcgag caagtgaaa ggccctaat 
      ccagcgccg acttgtatg gccctgttg gctaattta 
      gcgcaagtg tgtgcccgt ttttctgac ttacagccg 
      tacaatgat ggactgcga ggctgttta ctattagag 
      ccgaaattg ctgggcgat gttacagcg agttttaat 
      attggatgt tttacgatg gggcccgaa ggcatgaaa 
      ctggaagaa agtgttaat tccaccctc acgacactt 
      ctccagccc gaaaaaggc agcaacaat gccacgcag 
      agcgaatcg gaaggacta aatgaagag gaactaata 
      gccgctgta aatgagtcg gcagaacaa ggtcttgtt 
      ttcttcggg aatctacta ggtctaact ttcggaaaa 
      cccgaaacc aatgagact aataccgaa gctgtcgct 
      cctgctaca aatactagc aatgaaacg gcgacatct 
      acgtag
    mRNA sequence
    • >TC010803 RA
      tttcccagc taagccact cttggcact gaccaactc 
      aaaccgttg caccatgca ttgcgtgat atcacgatt 
      tgttgtact tcttgttgc attcactta tgtgtcttc 
      caccagtat cttagattt ctggaactg gggacacga 
      aggaagtga cttagaaca gagggatga caactcaga 
      ttcgacggc cctcagaac ttgcaaatg tgatggacc 
      gtcttgtat gtgctgcgt cgatttcaa tctgaccta 
      catcgacct gggagggcc aggttgtgt tcgaatgag 
      atacatttc gcaggacga agggattgc tgtcaacgt 
      ttcctatgg ggaaagtct tcttcacag cgagcaagt 
      gaaaggccc taatccagc gccgacttg tatggccct 
      gttggctaa tttagcgca agtgtgtgc ccgtttttc 
      tgacttaca gccgtacaa tgatggact gcgaggctg 
      tttactatt agagccgaa attgctggg cgatgttac 
      agcgagttt taatattgg atgttttac gatggggcc 
      cgaaggcat gaaactgga agaaagtgt taattccac 
      cctcacgac acttctcca gcccgaaaa aggcagcaa 
      caatgccac gcagagcga atcggaagg actaaatga 
      agaggaact aatagccgc tgtaaatga gtcggcaga 
      acaaggtct tgttttctt cgggaatct actaggtct 
      aactttcgg aaaacccga aaccaatga gactaatac 
      cgaagctgt cgctcctgc tacaaatac tagcaatga 
      aacggcgac atctacgta ggagacgtc acgtactcc 
      agaactcca caaattgat ccagattcc taatcatca 
      ctcgcactt atttatttc atcaaaaac ttgttttat 
      tatttattt aataaaata tttccctcg tt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY