Status
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Gene information
General information
TC031539
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010789,TC010788
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 5352452..5394856
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031539
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04910

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031539 RA
      MTSFNQDCV GGPGVVLMS FNIEEVLMR RVPRWRQDW 
      GISGLIYDK RERSRMPYQ LINSVTGQV QRASRLQLT 
      AIHREAAKK KSSACALFQ DLEMPEKRF QPPVSVKTR 
      KAVARVALN DNPRCALLA ISRDARIIY IREAMKQRR 
      WVAGAGGNS LRCEANEVS ATEAGDVRM TRGSASALR 
      RSLAALLAL DLIVVLGAA FQPEFAEPI VNLTIPMGR 
      DATFRCLVH HLGGYRVGW VKADTKAIQ AIHDHVITH 
      NPRVSVSHN DHTTWNLHI KNVQEEDRG QYMCQINTD 
      PMKSQLGFL DVVVPPDFI PEETSGDVM VPEGGTVKL 
      TCRARGHPE PHVQWRRED GSDIIIREP TGARTKVSS 
      YQGEVLLLV KISRSEMGA YMCIASNGV PPTVSKRIM 
      VNVNFHPVI QVPNQLVGA PLGTDVTLE CYVEASPKS 
      INYWVRDTG EMVIPSHKY DVQFVSKSL FEVRMTVIV 
      RNLQKEDAG SYRCIAKNS LGEVESNIR LYEIPGPTR 
      VYSLSLDEE DYNEQFGSA EREQDEELS NSVLERGRP 
      MSHTTPGTL RGTLTNTHE NQVPVDPAP SCSSRLVPM 
      LSSLLLLLL RI
    CDS sequence
    • >TC031539 RA
      atgacgagt tttaaccag gattgcgtc ggcggtccg 
      ggcgtcgtg ttaatgagt tttaacatc gaggaggtg 
      ttgatgcga cgtgtccca aggtggcgg caggactgg 
      ggcattagt ggcttgata tatgataag agggagcgc 
      tcccggatg ccttatcaa ctgattaac tctgtgact 
      ggccaagtg cagagagca tctcgtctg caattaacg 
      gccattcat cgggaggcg gcgaagaag aagagctcc 
      gcatgcgct ctgttccag gacttggaa atgccggag 
      aagcgcttc cagcctcca gtttccgtc aaaacgcgg 
      aaggctgtg gcgcgggtg gcccttaac gacaatccg 
      cgatgcgcc ttgctcgct attagccga gatgcacgg 
      ataatttat atcagagaa gcaatgaag caaagacga 
      tgggtggcc ggcgcgggc ggcaacagt ctgcggtgc 
      gaggctaat gaggtttcc gctaccgag gcaggagat 
      gtgagaatg acccggggg tcggcttca gccctgcgg 
      cggtccttg gctgcgctc ctcgcccta gacctcatc 
      gtcgtacta ggagcggcc ttccaaccc gaatttgca 
      gaacccatt gttaatcta accatcccc atgggacga 
      gatgccact ttccgatgt cttgtccac catttgggg 
      ggatacagg gtgggatgg gtgaaggcc gacaccaag 
      gcgatccag gcgatccac gaccacgtg atcacacac 
      aacccccga gtatcggtg tctcataac gaccacacc 
      acatggaat ctgcacatc aagaacgtg caagaggag 
      gacagaggg caatacatg tgtcagatt aacactgat 
      cccatgaaa agtcagctg ggctttctg gacgttgtc 
      gttcctccc gacttcatt cccgaggaa acttccgga 
      gatgtaatg gtacccgaa ggtgggact gtaaagttg 
      acttgtcga gcacggggc catcctgaa ccacatgta 
      caatggcga cgggaagat ggtagcgat attataata 
      agagagccc acgggagct cgcactaaa gtttcctcc 
      tatcaaggc gaagtgtta cttcttgtg aagatatca 
      cgttccgaa atgggcgca tatatgtgc atagcttca 
      aacggagtt cctccgacc gttagcaag aggattatg 
      gtcaatgta aatttccac ccggtaata caagtaccg 
      aatcaattg gtgggagcg ccattggga actgatgtg 
      acattagag tgttatgta gaggcttcc cctaaatcc 
      atcaactac tgggtgcga gataccggt gagatggtg 
      ataccgagc cataaatac gacgttcaa tttgtcagc 
      aagtcgcta tttgaagtg cgaatgacc gtaattgta 
      cggaacttg caaaaagaa gacgccgga tcctatcgc 
      tgcatcgcc aaaaactcg ctcggagaa gtagagagc 
      aacattcgc ctctacgaa attccaggt ccgacccgc 
      gtgtacagt ctctcgctg gacgaggaa gactataac 
      gagcagttc gggtctgct gagcgtgaa caggacgaa 
      gaattgtcg aacagtgtg cttgaacga ggccggccg 
      atgtcccac accactcct ggtacccta cggggtact 
      ctgacgaac acacatgag aaccaggtc cccgtcgac 
      ccggcgccg agctgctcc tccaggctg gtccccatg 
      ctgtcgtcg cttctgctg ctcctcctt aggatatga
    mRNA sequence
    • >TC031539 RA
      ggcgatgcg tcgtaatta tgacgagtt ttaaccagg 
      attgcgtcg gcggtccgg gcgtcgtgt taatgagtt 
      ttaacatcg aggaggtgt tgatgcgac gtgtcccaa 
      ggtggcggc aggactggg gcattagtg gcttgatat 
      atgataaga gggagcgct cccggatgc cttatcaac 
      tgattaact ctgtgactg gccaagtgc agagagcat 
      ctcgtctgc aattaacgg ccattcatc gggaggcgg 
      cgaagaaga agagctccg catgcgctc tgttccagg 
      acttggaaa tgccggaga agcgcttcc agcctccag 
      tttccgtca aaacgcgga aggctgtgg cgcgggtgg 
      cccttaacg acaatccgc gatgcgcct tgctcgcta 
      ttagccgag atgcacgga taatttata tcagagaag 
      caatgaagc aaagacgat gggtggccg gcgcgggcg 
      gcaacagtc tgcggtgcg aggctaatg aggtttccg 
      ctaccgagg caggagatg tgagaatga cccgggggt 
      cggcttcag ccctgcggc ggtccttgg ctgcgctcc 
      tcgccctag acctcatcg tcgtactag gagcggcct 
      tccaacccg aatttgcag aacccattg ttaatctaa 
      ccatcccca tgggacgag atgccactt tccgatgtc 
      ttgtccacc atttggggg gatacaggg tgggatggg 
      tgaaggccg acaccaagg cgatccagg cgatccacg 
      accacgtga tcacacaca acccccgag tatcggtgt 
      ctcataacg accacacca catggaatc tgcacatca 
      agaacgtgc aagaggagg acagagggc aatacatgt 
      gtcagatta acactgatc ccatgaaaa gtcagctgg 
      gctttctgg acgttgtcg ttcctcccg acttcattc 
      ccgaggaaa cttccggag atgtaatgg tacccgaag 
      gtgggactg taaagttga cttgtcgag cacggggcc 
      atcctgaac cacatgtac aatggcgac gggaagatg 
      gtagcgata ttataataa gagagccca cgggagctc 
      gcactaaag tttcctcct atcaaggcg aagtgttac 
      ttcttgtga agatatcac gttccgaaa tgggcgcat 
      atatgtgca tagcttcaa acggagttc ctccgaccg 
      ttagcaaga ggattatgg tcaatgtaa atttccacc 
      cggtaatac aagtaccga atcaattgg tgggagcgc 
      cattgggaa ctgatgtga cattagagt gttatgtag 
      aggcttccc ctaaatcca tcaactact gggtgcgag 
      ataccggtg agatggtga taccgagcc ataaatacg 
      acgttcaat ttgtcagca agtcgctat ttgaagtgc 
      gaatgaccg taattgtac ggaacttgc aaaaagaag 
      acgccggat cctatcgct gcatcgcca aaaactcgc 
      tcggagaag tagagagca acattcgcc tctacgaaa 
      ttccaggtc cgacccgcg tgtacagtc tctcgctgg 
      acgaggaag actataacg agcagttcg ggtctgctg 
      agcgtgaac aggacgaag aattgtcga acagtgtgc 
      ttgaacgag gccggccga tgtcccaca ccactcctg 
      gtaccctac ggggtactc tgacgaaca cacatgaga 
      accaggtcc ccgtcgacc cggcgccga gctgctcct 
      ccaggctgg tccccatgc tgtcgtcgc ttctgctgc 
      tcctcctta ggatatgac gacccgctg ttcccgacg 
      catttatgt agtgacaat aacgtttca ttcctttgt 
      tcccgatta ttgaccgtt tttatttca aaacccagt 
      ttcatgtgt ggttttgat tgtatagtc taattcatg 
      tatggtaaa ctaaacgta gctgggcca ccaccgtgg 
      cgccgggag accatacag tatgttgcc ttataatat 
      atgtcaaat aacttagat tcgtaagac attttaggc 
      agtctagtt ttacggaaa tatcatata aatatgtta 
      aaccagttg tttgctgaa atgtattaa ttattatat 
      gttattctg ggatttaag tcatgcaaa taaatacga 
      taagtcctc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY